CN110384110B

CN110384110B - Method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens and application

Info

Publication number: CN110384110B
Application number: CN201810382778.1A
Authority: CN
Inventors: 刘罡; 白建军; 常稳
Original assignee: Bayer CropScience China Co Ltd
Current assignee: Bayer CropScience China Co Ltd
Priority date: 2018-04-23
Filing date: 2018-04-23
Publication date: 2021-08-31
Anticipated expiration: 2038-04-23
Also published as: CN110384110A

Abstract

The invention relates to application and a method for preventing and controlling tomato yellow leaf curl disease by bacillus amyloliquefaciens, wherein the bacillus amyloliquefaciens is sprayed on tomato leaves at an application rate of 1.67 multiplied by 10¹¹‑5.00×10¹¹cfu/mu. The bacillus amyloliquefaciens starts to be applied after the tomatoes are transplanted and slow-released, and is continuously applied for 3 times after 10 days; preferably, the administration is continued 5 times after 7 days. The bacillus amyloliquefaciens is 1 multiplied by 10⁹Applied as a suspension of cfu/ml. Compared with other formulations such as wettable powder, the suspending agent is safe for crops and does not pollute fruit surfaces.

Description

Method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens and application

Technical Field

The invention relates to a method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens and application of the bacillus amyloliquefaciens in preventing and controlling tomato yellow leaf curl disease.

Background

Tomato Yellow Leaf Curl Virus (TYLCV) is a plant single-stranded DNA virus and can occur in the flowering stage, fruiting stage and harvest stage of tomatoes. After tomato plants are infected with viruses, the tomato plants mainly show retarded or arrested growth at the initial stage, yellowing of growing points, shortening of internodes, obvious dwarfing of plants, reduction and thickening of leaves, crisp and hard leaf quality, folding and upward curling of leaves, yellowing of leaf areas from the edges of the leaves to leaf veins, typical symptoms of the leaves at the upper part of the plants and unobvious symptoms of old leaves at the lower part. The later stage shows that the fruit setting is less, the fruit becomes smaller, the expanding speed is slow, the fruit in the mature period can not be normally changed in color, and the like. After tomato plants are infected with viruses, especially before flowering, the fruit yield and commodity value are greatly reduced. The loss caused by serious disease can reach 100 percent, and the tomato plant disease is one of the diseases seriously harming tomato production.

The Altailing and the lentinan are medicaments approved for preventing and controlling the tomato yellow leaf curl disease at present. It would be desirable to provide a ready-to-use medicament for the prevention and control of tomato yellow leaf curl disease.

Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is a gram-positive bacterium, can secrete various extracellular antibacterial substances, has an inhibiting effect on plant pathogenic bacteria, fungi, viruses and nematodes, can remarkably improve the disease resistance of plants, can promote the growth of the plants and improve the yield of the plants, is a good biocontrol growth-promoting bacterium, and particularly has a good inhibiting effect on bacterial wilt and mosaic disease. For example, chinese literature "study on inhibition of accumulation of tobacco mosaic virus RNA in leaves by biocontrol bacterium Ba33, plant protection, 2010, 36 (3): 82-84 'and' the growth promoting and anti-TMV effect of Bacillus amyloliquefaciens Ba33 on tobacco, the journal of Jilin university of agriculture, 2010, 32(40): 383-.

Chinese patent application CN102160553A discloses a Ba preparation against plant virus disease containing biocontrol bacteria Ba33, and verifies the efficacy of the preparation against tobacco mosaic virus: the greenhouse simulation is 80 percent, and the field control effect is 49.09 percent.

At present, no report on the use of bacillus amyloliquefaciens for preventing and controlling DNA virus diseases such as tomato yellow leaf curl disease exists.

QST713 under the trade name of

Is a viable bacteria preparation developed by the American agrequest company. This bacterium was originally classified as a member of the species Bacillus subtilis. The bacillus subtilis is a past extremely large flora with similar shapes, and the bacillus subtilis is identified as the bacillus amyloliquefaciens through repeated identification along with the improvement and updating of identification technologies and comparison databases in recent years. Serenade is approved and applied not only by the grade of the United states environmental protection agency, but also by the disease application of edible crops, and can prevent and control diseases caused by bacteria and fungi such as powdery mildew, root rot, downy mildew, epidemic disease, gray mold and the like of various crops such as vegetables, cherries, grapes, cucurbits and walnuts by applying to leaf surfaces. At present, no report exists on the application of the strain in preventing and controlling virus diseases, particularly DNA virus diseases such as tomato yellow leaf curl disease.

Disclosure of Invention

The inventor of the invention finds that the bacillus amyloliquefaciens can also be used for preventing and controlling tomato yellow leaf curl disease.

An object of the invention is to provide the application of bacillus amyloliquefaciens in preventing and controlling tomato yellow leaf curl diseaseUse, wherein the bacillus amyloliquefaciens is sprayed on tomato leaves at an application rate of 1.67 x 10¹¹-5.00×10¹¹cfu/mu.

In one embodiment of the use of the present invention, the bacillus amyloliquefaciens is QST 713.

In another embodiment of the application of the invention, the bacillus amyloliquefaciens is applied for 3 times after 10 days of interval from the beginning of the application after the transplanting of the tomatoes and the slow seedlings.

In a preferred embodiment of the application of the invention, the bacillus amyloliquefaciens is applied for 5 times after the tomato is transplanted to the slow seedling and the application is started after 7 days.

In a preferred embodiment of the use according to the invention, the Bacillus amyloliquefaciens is present in a ratio of 1X 10⁹Applied as a suspension of cfu/ml.

Another object of the present invention is to provide a method for preventing and controlling tomato yellow leaf curl disease using Bacillus amyloliquefaciens, wherein the Bacillus amyloliquefaciens is sprayed on tomato leaves at an application rate of 1.67 x 10¹¹-5.00×10¹¹cfu/mu.

In one embodiment of the method of the present invention, the Bacillus amyloliquefaciens is QST 713.

In another embodiment of the method of the present invention, the bacillus amyloliquefaciens is applied after transplanting the tomato seedlings for 3 times after 10 days.

In a preferred embodiment of the method of the invention, the bacillus amyloliquefaciens is applied for 5 times after the tomato is transplanted to the seedlings and the application is started after 7 days.

In a preferred embodiment of the method of the invention, the Bacillus amyloliquefaciens is present in a ratio of 1X 10⁹Applied as a suspension of cfu/ml.

Compared with other formulations such as wettable powder, the suspending agent is safe for crops and does not pollute fruit surfaces.

Advantageous effects

The method for preventing and controlling tomato yellow leaf curl disease has the following advantages:

(1) the strain activity is high;

(2) the suspending agent is convenient to use and does not pollute fruit surfaces;

(3) is suitable for green prevention and control and organic agricultural production;

(4) is beneficial to the drug resistance management of chemical pesticides;

(5) the residue risk of chemical pesticides is reduced.

The following examples are for the purpose of illustrating the invention only and are not to be construed as limiting the invention. Those skilled in the art will recognize that changes may be made without departing from the spirit of the invention, the scope of which is defined by the claims.

Example 1

The test was carried out in tomato fields of western village, Zhenggao village, Jianshui county, Yunnan province. Transplanting in 25 days of 7 months, and planting about 2000 plants per mu in 45 plants/area. No bactericide is used 1 month before the medicine.

Tomato leaves were sprayed with the agent as shown in table 1 below. Wherein 1.34% (w/w) Serenade SC (1X 10)⁹cfu/ml suspension) was used AS a test agent, 6% (w/w) of Altai Ling WP and 1% (w/w) of lentinan AS were used AS a control agent. All agents are commercially available products.

Table 1: dosage, timing and method of administration of a medicament

The cell area was 4m × 3.65m — 14.6 m in square, three replicates. And protective rows are arranged among the cells.

The primary pesticide application is carried out 25 days after the seedlings are transplanted and the seedlings are delayed within 7 months and 25 days, namely within 8 months and 20 days; 8, 27 days, the second application; 9 months and 3 days, and the third application. The application is carried out 3 times in total.

The application method specifically adopts a spraying method, and manual spraying is carried out by using a knapsack electric sprayer produced by a yellow rock green garden sprayer factory in Taizhou city. The preparation of the liquid medicine adopts a secondary dilution method: dissolving the preparation with a small amount of water, pouring into a manual sprayer, adding water, shaking uniformly, and spraying uniformly on the whole tomato plant. The water consumption of each district is consistent when the pesticide is applied each time, and the water consumption of each mu is 50 kilograms when the pesticide is applied each time.

The prevention and control effect is investigated when the disease condition is stable. And (4) surveying all plants in each cell, and recording and surveying the total number of the plants and the number of the sick plants. The base of each pre-dose investigation was 8/20/8/27/9/3/2 on each day, and 2 investigations were carried out on 9/13/9 (10 days after the last dose).

The specific classification method (in units of plants) is as follows:

level 0: the plant has no obvious symptoms and grows normally;

level 1: the edges of 1-3 leaves at the top of the plant are slightly curled;

and 2, stage: the leaves above 1/3 are curled and shrunk, the space between the upper leaf nodes is shortened, and the leaves are shorter than the plants without diseases;

and 3, level: the leaves above 1/2 are obviously curled and shrunk, the pitch of the upper leaves is shortened, and the plants are dwarfed;

4, level: the leaves above 2/3 are obviously curled and shrunk, the leaf pitch of the upper part is shortened, and the plants are obviously dwarfed;

calculating disease index and prevention effect according to the pesticide field efficacy test criteria through the following formulas:

the disease index and the prevention and control effect are detailed in table 2:

TABLE 1Serenade field efficacy in the prevention and control of tomato yellow leaf curl disease

Note: the data in the table are the average of three replicates.

The tomato is safe to tomatoes, the tomato grows normally when the test agent is in the test dosage range, no phytotoxicity phenomenon is observed, and no influence on other organisms is observed.

The disease index of a blank control reaches 20.46 and the disease index of medicament treatment is 6.6-9.71 when the investigation is carried out 10 days after the last application, and the protective effect on upper leaves and newly born leaves is better. The agent treated area is significantly better in growth than the blank control area.

Statistical data show that: compared with a contrast medicament of 6 percent of Altai ling WP 100 g/mu and 1 percent of lentinan AS 250 ml/mu, the Serenade has better prevention and control effects on tomato yellow leaf curl disease.

Claims

1. Use of bacillus amyloliquefaciens for preventing and controlling tomato yellow leaf curl disease, wherein the bacillus amyloliquefaciens is sprayed on tomato leaves at an application rate of 1.67 multiplied by 10¹¹-5.00×10¹¹cfu/acre, wherein said Bacillus amyloliquefaciens is QST 713.

2. The use according to claim 1, wherein the bacillus amyloliquefaciens is administered 3 times after 10 days after the tomato transplantation.

3. The use according to claim 1, wherein the bacillus amyloliquefaciens is administered 5 times after the tomato transplantation seedling and after 7 days.

4. The use according to claim 1, wherein the bacillus amyloliquefaciens is present in a 1 x 10 ratio⁹Administration is in the form of cfu/mL suspension.

5. Method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens, wherein the bacillus amyloliquefaciens is sprayed on tomato leaves at an application rate of 1.67 multiplied by 10¹¹-5.00×10¹¹cfu/acre, wherein said Bacillus amyloliquefaciens is QST 713.

6. The method according to claim 5, wherein the application of Bacillus amyloliquefaciens is started after the transplanting of the tomato seedlings and is continued for 3 times after 10 days.

7. The method according to claim 5, wherein the application of Bacillus amyloliquefaciens is started after the transplanting of the tomato seedlings and is continued for 5 times after 7 days.

8. The method according to claim 5, wherein said Bacillus amyloliquefaciens is present in a range of 1 x 10⁹Administration is in the form of cfu/mL suspension.