CN110384110A - The method and purposes of bacillus amyloliquefaciens prevention and control tomato yellow leaf curl - Google Patents
The method and purposes of bacillus amyloliquefaciens prevention and control tomato yellow leaf curl Download PDFInfo
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- CN110384110A CN110384110A CN201810382778.1A CN201810382778A CN110384110A CN 110384110 A CN110384110 A CN 110384110A CN 201810382778 A CN201810382778 A CN 201810382778A CN 110384110 A CN110384110 A CN 110384110A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The present invention relates to the purposes and method of bacillus amyloliquefaciens prevention and control tomato yellow leaf curl, wherein the bacillus amyloliquefaciens are sprayed on tomato leaf, rate of application is 1.67 × 1011‑5.00×1011Cfu/ mus.The bacillus amyloliquefaciens start to be administered after tomato transplants slow seedling, and interval is after 10 days, continuous application 3 times；After 7 days, continuous application 5 times.The bacillus amyloliquefaciens are with 1 × 109The suspending agent form of cfu/ml is administered.For other dosage forms such as wettable powder, suspending agent will not pollute fruit face to crop safety.
Description
Invention field
The present invention relates to a kind of methods with bacillus amyloliquefaciens prevention and control tomato yellow leaf curl, and solution starch gemma
The purposes of bacillus prevention and control tomato yellow leaf curl.
Background technique
Tomato yellow leaf curl virus disease (TYLCV), is a kind of plant single-stranded DNA viruses, the disease is at tomato florescence, result
Phase, picking time in each stage can occur.After tomato plant virus infection, initial stage mainly shows growth retardation or stagnation, growing point
Yellow, internode shorten, and plant is obviously downgraded, and blade, which becomes smaller, to thicken, and the food value of leaf is brittle, and blade has fold, upsweeps, blade edge
Typical with plant upper blade symptom to the yellow of vein region, lower part old leaf symptom is unobvious.Later period performance is beared fruit less, fruit
Become smaller, expand that speed is slow, the fruit in maturity period cannot normal annesl etc..After tomato plant virus infection, especially before flowering
Virus infection, fruit yield and the equal sharp fall of commodity value.Loss caused by when serious is to seriously endanger up to 100%
One of disease of tomato production.
Ata spirit and lentinan are the medicaments for being approved prevention and control tomato yellow leaf curl at present.It is desirable to provide for prevention and control kind
The spare medicament of eggplant tomato yellow leaf curl China.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are a kind of gram-positive bacterias, can be secreted more
Kind extracellular antiseptic substance, has inhibiting effect to plant pathogenetic bacteria, fungi, virus, nematode, significantly improves the disease resistance of plant,
It can promote plant growth simultaneously, improve the yield of plant, be a kind of good biological and ecological methods to prevent plant disease, pests, and erosion Promoting bacteria, especially to bacterial wilt, mosaic disease
There is fine inhibiting effect.For example, China's document " research that biocontrol microorganisms Ba33 inhibits tobacco mosaic virus RNA to accumulate in blade,
Plant protection, 2010,36 (3): 82-84 " and " bacillus amyloliquefaciens Ba33 acts on the growth-promoting of tobacco and anti-TMV, Jilin
Agriculture university's journal, 2010,32 (40): 383-386 ", which describes bacillus amyloliquefaciens Ba, has tobacco mosaic virus (TMV) (TMV)
There is inhibiting effect.
The bacillus amyloliquefaciens preparation for controlling viral diseases of plants comprising biocontrol microorganisms Ba33 is disclosed in Chinese patent application CN102160553A, and
Demonstrate imitation of the said preparation to tobacco mosaic virus (TMV): greenhouse imitates 80%, crop field preventive effect 49.09%.
There has been no the reports that bacillus amyloliquefaciens are used for prevention and control DNA virus disease such as tomato yellow leaf curl at present.
QST713, trade nameIt is the active bacteria formulation that AgraQuest company, the U.S. developed.The bacterium is most
Just it is classified as Bacillus subtillis strain member.Better than Bacillus subtillis be it is past, one greatly, with similar figures
The flora of shape, and with identification technology in recent years and compare the improvement and update of database, the bacterium identified repeatedly have confirmed that for
Bacillus amyloliquefaciens.Serenade not only passes through the grade of U.S.EPA but also obtains examining for alimentary crop disease application
Batch and application, by the powdery mildew of foliage applying energy prevention and control vegetables, cherry, grape, cucurbit and English walnut various crop, root rot,
The bacteriums such as downy mildew, epidemic disease, gray mold and fungus-caused disease.It also is used for prevention and control virosis without the bacterial strain at present, especially
It is the report of DNA virus disease such as tomato yellow leaf curl.
Summary of the invention
Present inventors found that bacillus amyloliquefaciens can also be used to prevention and control tomato yellow leaf curl.
It is an object of the present invention to provide the purposes of bacillus amyloliquefaciens prevention and control tomato yellow leaf curl, wherein described
Bacillus amyloliquefaciens are sprayed on tomato leaf, and rate of application is 1.67 × 1011-5.00×1011Cfu/ mus.
In an embodiment of purposes of the present invention, the bacillus amyloliquefaciens are QST713.
In another embodiment of purposes of the present invention, the bacillus amyloliquefaciens are opened after tomato transplants slow seedling
Begin to be administered, interval is after 10 days, continuous application 3 times.
In a preferred embodiment of purposes of the present invention, the bacillus amyloliquefaciens are after tomato transplants slow seedling
Start to be administered, interval is after 7 days, continuous application 5 times.
In a preferred embodiment of purposes of the present invention, the bacillus amyloliquefaciens are with 1 × 109Cfu/ml's
The application of suspending agent form.
It is a further object to provide a kind of method with bacillus amyloliquefaciens prevention and control tomato yellow leaf curl,
Wherein the bacillus amyloliquefaciens are sprayed on tomato leaf, and rate of application is 1.67 × 1011-5.00×1011Cfu/ mus.
In an embodiment of the method for the present invention, the bacillus amyloliquefaciens are QST713.
In another embodiment of the method for the present invention, the bacillus amyloliquefaciens are opened after tomato transplants slow seedling
Begin to be administered, interval is after 10 days, continuous application 3 times.
In a preferred embodiment of the method for the present invention, the bacillus amyloliquefaciens are after tomato transplants slow seedling
Start to be administered, interval is after 7 days, continuous application 5 times.
In a preferred embodiment of the method for the present invention, the bacillus amyloliquefaciens are with 1 × 109Cfu/ml's
The application of suspending agent form.
For other dosage forms such as wettable powder, suspending agent will not pollute fruit face to crop safety.
Beneficial effect
The method of prevention and control tomato yellow leaf curl of the present invention has the advantage that
(1) bacterial activity is high；
(2) suspending agent is easy to use, and does not pollute fruit face；
(3) it is suitable for green prevention and control and organic agriculture production；
(4) be conducive to the drug resistance management of chemical pesticide；
(5) the residual risk of chemical pesticide is reduced.
The following example is only used for the purpose illustrated the present invention and does not limit the present invention.Those skilled in the art can
To make variation without departing substantially from substantive content of the present invention in the cognitive range of oneself.
Embodiment 1
Test is implemented in the village the Gao Ying tomato ground of Yunnan Province, the town Xi Zhuan, Jianshui County.Transplanting on July 25,45 plants/area, mu is planted about
2000 plants.1 month not used any fungicide before medicine.
Medicament is sprayed to tomato leaf, as shown in table 1 below.Wherein 1.34% (w/w) Serenade SC (1 × 109cfu/
Ml suspension) it is used as tested medicament, 6% (w/w) ata spirit WP and 1% (w/w) lentinan AS are used as comparison medicament.All medicines
Agent is commercial product.
Table 1: pharmacy application amount, spraying time and method
Plot area is 4m × 3.65m=14.6 square metres, is repeated three times.Minizone sets protection row.
25 days after transplanting on July 25 slow seedling, i.e. August initial administration on the 20th；August 27 days, second of application；September 3 days, the
It is administered three times.It is administered 3 times altogether.
Application specifically uses spray-on process, and the knapsack electric sprayer produced with Taizhou plain Huangyan Lv Yuan spraying machine factory is manual
It is spraying.Preparating liquid uses double dilution method: a small amount of water pharmaceutical dissolution first used, then pours into hand sprayer and water is added to shake up, it is right
Tomato complete stool even spraying.It is consistent that it is administered every cell water consumption every time, water consumption is 50 kilograms per acre for application every time.
Control effect is investigated when aobvious disease to stable disease.Every cell investigates whole plant, record investigation total strain number, diseased plant
Number.Radix is investigated within August 20, August 27 days, September 3rd before each medicine, is carried out 2 times within September 13rd (after last medicine 10 days)
Investigation.
Specific stage division (as unit of strain) is as follows:
0 grade: plant does not have manifest symptom, and growth is normal；
1 grade: slight curling occurs for 1-3 piece blade edge at the top of plant；
2 grades: the leaf rolling of 1/3 or more plant, shrinkage, upper blade section hypotelorism are shorter and smaller than not susceptible plant；
3 grades: with blade obvious curling, shrinkage, upper blade section hypotelorism occur for plant 1/2, and plant is downgraded；
4 grades: plant 2/3 obviously crimps with blade, shrinkage, upper blade section hypotelorism, and plant is downgraded obvious；
Disease index and preventive effect are calculated by the following formula according to " pesticide field efficacy medicine test criterion ":
See Table 2 for details for disease index and control effect:
Table 1Serenade prevention and control tomato yellow leaf curl field efficacy
Note: data are to repeat average value three times in table.
To tomato safety, within the range of experimental dose, tomato growth is normal for reagent agent, does not observe phytotoxicity phenomenon,
The influence to other biologies is not observed.
It investigates within 10 days after last application, for blank control disease index up to 20.46, chemicals treatment disease index is 6.6.-
9.71, it is preferable to upper blade and newborn blade protective effect.Chemicals treatment district growing way is substantially better than blank district.
Statistical data is shown: with 100 grams/acre of 6% ata spirit WP of comparison medicament and 250 milliliters of 1% lentinan AS/
Mu is compared, and Serenade is more excellent to the control effect of tomato yellow leaf curl.
Claims (10)
1. the purposes of bacillus amyloliquefaciens prevention and control tomato yellow leaf curl, wherein the bacillus amyloliquefaciens are sprayed on tomato
Blade, rate of application are 1.67 × 1011-5.00×1011Cfu/ mus.
2. purposes according to claim 1, wherein the bacillus amyloliquefaciens are QST713.
3. purposes according to claim 1 or 2, wherein the bacillus amyloliquefaciens start to apply after tomato transplants slow seedling
Medicine, interval is after 10 days, continuous application 3 times.
4. purposes according to claim 1 or 2, wherein the bacillus amyloliquefaciens start to apply after tomato transplants slow seedling
Medicine, interval is after 7 days, continuous application 5 times.
5. purposes according to claim 1, wherein the bacillus amyloliquefaciens are with 1 × 109The suspending agent form of cfu/mL is applied
Medicine.
6. the method for bacillus amyloliquefaciens prevention and control tomato yellow leaf curl, wherein the bacillus amyloliquefaciens are sprayed on tomato
Blade, rate of application are 1.67 × 1011-5.00×1011Cfu/ mus.
7. method according to claim 5, wherein the bacillus amyloliquefaciens are QST713.
8. according to the method for claim 5 or 6, wherein the bacillus amyloliquefaciens start to apply after tomato transplants slow seedling
Medicine, interval is after 10 days, continuous application 3 times.
9. according to the method for claim 5 or 6, wherein the bacillus amyloliquefaciens start to apply after tomato transplants slow seedling
Medicine, interval is after 7 days, continuous application 5 times.
10. purposes according to claim 6, wherein the bacillus amyloliquefaciens are with 1 × 109The suspending agent form of cfu/mL
Application.
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| CN201810382778.1A CN110384110B (en) | 2018-04-23 | 2018-04-23 | Method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens and application |
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| CN201810382778.1A CN110384110B (en) | 2018-04-23 | 2018-04-23 | Method for preventing and controlling tomato yellow leaf curl disease by using bacillus amyloliquefaciens and application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635921A (en) * | 2017-01-17 | 2017-05-10 | 陈刘军 | Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15 |
| CN107858300A (en) * | 2017-05-31 | 2018-03-30 | 南京农业大学 | For the diseases prevention of tomato, growth-promoting, quality-improving and degeneration-resistant bacillus amyloliquefaciens 2YN11 and its application |
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- 2018-04-23 CN CN201810382778.1A patent/CN110384110B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635921A (en) * | 2017-01-17 | 2017-05-10 | 陈刘军 | Disease-preventing, growth-promoting and stress-resisting bacillus amyloliquefaciens Y15 for all plants and application of bacillus amyloliquefaciens Y15 |
| CN107858300A (en) * | 2017-05-31 | 2018-03-30 | 南京农业大学 | For the diseases prevention of tomato, growth-promoting, quality-improving and degeneration-resistant bacillus amyloliquefaciens 2YN11 and its application |
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