CN110354927A - A kind of extensive micro drop arrangement apparatus and method of gravity drive - Google Patents
A kind of extensive micro drop arrangement apparatus and method of gravity drive Download PDFInfo
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- CN110354927A CN110354927A CN201910648886.3A CN201910648886A CN110354927A CN 110354927 A CN110354927 A CN 110354927A CN 201910648886 A CN201910648886 A CN 201910648886A CN 110354927 A CN110354927 A CN 110354927A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
- B01J2/02—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0244—Drop counters; Drop formers using pins
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/02—Laboratory benches or tables; Fittings therefor
Abstract
The present invention provides the extensive micro drop arrangement apparatus and method of a kind of gravity drive, for the high-precision micro drop of arrangement of the specific position on sample.The device includes: sample, liquid-supplying system, kinetic control system and a NI Vision Builder for Automated Inspection with special hydrophilic/super-hydrophobic array structure.The device utilizes dropping liquid gravity and hydrophilic/super-hydrophobic array structure double action of sample surfaces, realizes the exact placement of micro drop.The volume of its drop of arranging is determined by the shapes and sizes of the single hydrophilic region of sample surfaces.Arrangement process is linear, and a variety of different liquid that can arrange simultaneously.Meanwhile the drop arrangement apparatus structure is simple, arrangement is high-efficient, scalability is strong, is pin-pointed to arrangement position without injection needle, it can be achieved that the micro drop arrangement of large-scale nanoliter level.
Description
Technical field
The present invention relates to micro drop arrangement fields, and in particular to a kind of extensive micro drop arrangement dress of gravity drive
It sets and method.
Background technique
Accurate micro drop arrangement is the multiple fields urgent needs such as biomedicine, chemistry, microfluid, material science
, especially in fields such as cell culture, biological microoperation, chemical reaction, materials synthesis, micro drop arrangement has important answer
With value.However, existing drop arrangement mode can not meet high-precision and efficient requirement simultaneously, there is an urgent need to can be real
The method of now extensive micro drop arrangement.
Summary of the invention
Low efficiency, positioning accuracy are poor, arrangement liquid is single for arranging present in micro drop arrangement at present by the present invention
The defects of, the extensive micro drop arrangement apparatus and method of a kind of gravity drive are provided, for having wetting sex differernce
Hydrophilic/super-hydrophobic array structure surface arrange micro drop.
The present invention provides a kind of extensive micro drop arrangement methods of gravity drive, while additionally providing a drop
Arrangement apparatus.The device includes: a sample with special hydrophilic/super-hydrophobic array structure, droplet injection system, movement control
System, NI Vision Builder for Automated Inspection and computer control system processed.
The sample of the invention has the function of the micro drop of arrangement by special designing, surface be equipped with by hydrophilic region with
Super-hydrophobic region.Wetting sex differernce of the sample using liquid to be arranged in hydrophilic region and super-hydrophobic region realizes microtitre
Arrangement.When liquid to be arranged flows through hydrophilic region, the liquid of designated volume is retained in hydrophilic region, and the liquid of reservation is based on
Surface tension of liquid effect is spontaneous to form drop in hydrophilic region.When liquid to be arranged flows through super-hydrophobic region, without liquid
It is retained in the region.The droplet size of reservation is determined by the shapes and sizes of sample surfaces hydrophilic region.Meanwhile hydrophilic region
It is arranged into array structure with super-hydrophobic region, hydrophilic region is surrounded completely by super-hydrophobic region.Utilize this of the sample surfaces
Special hydrophilic/super-hydrophobic array structure realizes the high-precision parent for being arranged in sample surfaces of micro drop designated volume
Water area.The sample is rectangle, etches hydrophilic region by the way that ultraviolet photolithographic technology is high-precision in superhydrophobic substrate.Guarantee
The shapes and sizes of hydrophilic region, and then ensure that the volume of arrangement drop, obtain there is hydrophilic/super-hydrophobic array structure
Sample.The sample shape, size can further expand simultaneously.
Dropping liquid injecting systems include: injection needle (1), are connected by needle adapter (2) and eluate conduit (3).Out
The other end of fluid catheter (3) is connected with three-way switch valve (4) left end interface.Three-way switch valve (4) right end interface and liquid inlet conduit
(5) it is connected, lower end interface is connected with plunger pump (6).Liquid inlet conduit (5) end connects a liquid impurity filter (7), is used for
Filter the impurity in liquid storage bottle (8) liquid.Liquid inlet conduit (5) is fixed by the guide hole that liquid storage bottle covers.Meanwhile liquid storage bottle cap
There are one air filtration hole, air filtering plug (9) are housed in hole.
Kinetic control system includes two parts of syringe needle positioning system and sample inclination system.Syringe needle positioning system uses X
(12) three axis motorized precision translation stage (10), Y-axis motorized precision translation stage (11) and Z axis motorized precision translation stage motorized precision translation stages (10) (11)
(12) accurate positioning of syringe needle is realized.Wherein, injection needle (1) is connected with needle adapter (2).Needle adapter (2) passes through
Hand screw (13) is fixed on needle mount (14).Needle mount (14) is connected to Z axis by right-angle connector (15)
On motorized precision translation stage (12).Two motorized precision translation stages of YZ axis are connected using right-angle connector (16).Two of XY axis are electronic flat
Moving stage is connected using connecting plate (17).Three motorized precision translation stages (10) (11) (12) of XYZ axis are for controlling the three of injection needle
Maintenance and operation is dynamic, makes the predeterminated position on injection needle (1) precise motion to sample.
Sample inclination system tilts special angle using electric rotary table (18) control sample (19).Wherein, sample
(19) it is placed on sample stage (20).Sample stage (20) is connect with electric rotary table (18), and electric rotary table (18) passes through rotation
Platform fixing piece (21) is fixed on vibration-damped table (22).For tilting sample (19), sample is held electric rotary table (18) on (19)
Drop to be arranged.Using the double action of drop gravity and special hydrophilic/super-hydrophobic array structure of sample (19), realize micro-
Measure the exact placement of drop.Initial stage, sample (19) keep horizontal positioned.
NI Vision Builder for Automated Inspection passes through a colorful CCD camera (23) and microspur zoom lens (24) observing samples (19)
The drop of upper arrangement.Colorful CCD camera (23) is fixed on camera support (26) by camera connecting plate (25).Camera support
(26) it is fixed on vibration-damped table (22) by right-angle connector (27).
Computer control system controls the accurate control that computer realizes the micro drop arrangement apparatus by a laboratory.
Control unit includes wherein tri- axis electricity driving displacement platform (10) (11) (12) of XYZ, electric rotary table (18), plunger pump (6), colour
CCD camera (23).According to the program write, successively carried out by step.Wherein, tri- axis electricity driving displacement platform (10) (11) of XYZ
(12) for controlling the three-dimensional motion of injection needle (1)；Electric rotary table (18) is used to control the tilt angle of sample stage (20)；
Plunger pump (6) realizes the drop of injection needle (1) injection particular size volume；Colorful CCD camera (23) is for observing syringe needle
The drop arranged on real time position and sample (19).
Further, which further includes a waste collection box (28), is placed on vibration-damped table (22), for collecting
Surplus liquid.
Further, the device X-axis motorized precision translation stage (10) and turntable fixing piece (20) are fixed by soket head cap screw
On vibration-damped table (22).
Further, drop annotation syringe needle (1) internal diameter is 100 microns, for injecting the drop of determined volume on sample.
Further, liquid to be arranged can choose a variety of different liquid, and can arrange a variety of different liquid simultaneously.
Further, the surface area of the device sample and shape can extend.It, can be primary by extending surface area of sample
Realize more massive drop arrangement.By extending the shape of sample, the device can be made to adapt to different application environments, widened
The selection range of sample.
Invention also provides a kind of extensive micro drop arrangement methods of gravity drive, and drop arrangement method is such as
Under:
1) mounting device various components, connecting conduit.
2) pass through three-D electric translation stage, mobile injection needle (1) to drop arrangement initial default location.
3) it is fixed on sample stage (20) sample (19).
4) three-way switch valve (4) switches to plunger pump (6) and liquid inlet conduit (5) connected state, plunger pump work, from liquid storage
It is drawn inside experimental liquid to Plunger pump body in bottle (8).
5) three-way switch valve (4) switches to plunger pump (6) and eluate conduit (3) connected state, plunger pump work, plunger
The liquid conveying of pump body is pumped to injection needle (1).
6) above-mentioned two steps operation is repeated, discharge liquid inlet conduit (5), three-way switch valve (4), plunger pump (6), liquid is led out
Air in pipe (3), needle adapter (2) and injection needle (1).The liquid of injection needle (1) discharge is dropped in waste collection
In box (28).
7) three-D electric translation stage (10) (11) (12) controls injection needle (1) precise motion to operating position, plunger pump
(6) it works, the predeterminated position on sample (19) surface injects the dropping liquid to be arranged (29) of one group of 0.15ml size.
8) electric rotary table (18) moves, and tilts sample (19) towards specific direction.When tilt angle is greater than sample
(19) when keeping the critical value of drop, drop is because gravity quickly glides.Meanwhile sample (19) utilizes the parent of its own
Water/super-hydrophobic array structure keeps micro drop (31) in the hydrophilic region (30) that drop passes through, super-hydrophobic region (32) no
Keep drop.Thus realize the micro drop of the arrangement designated volume of the hydrophilic region high-precision automatic on sample (19),
Its volume size is determined by the shapes and sizes of hydrophilic array structure.
The invention has the benefit that the present invention be directed to arrangement low efficiency present in current micro drop arrangement apparatus,
The defects of positioning accuracy is poor, arrangement liquid is single provides extensive micro drop arrangement apparatus and the side of a kind of gravity drive
Method, for the micro drop of hydrophilic position arrangement on hydrophilic/super-hydrophobic array structure surface with wetting sex differernce.The arrangement
Apparatus structure is simple, arrangement droplet size is accurate, arrangement is high-efficient, arrangement position is pin-pointed to without injection needle.Simultaneously
Scalability is strong, it can be achieved that large-scale micro drop arrangement, all has weight for biomedical, chemistry, microfluid etc.
Want application value.
Detailed description of the invention
The extensive micro drop arrangement apparatus work flow diagram of Fig. 1 gravity drive.
The extensive micro drop arrangement apparatus structural schematic diagram of Fig. 2 gravity drive.
Hydrophilic/super-hydrophobic array structure schematic diagram of Fig. 3.
The micro drop arrangement process schematic of Fig. 4.
Fig. 5 is the schematic diagram of the sampler of the interfacial contact formula based on the principle of the invention.
Fig. 6 is the schematic diagram of the sampler of the interface immersion based on the principle of the invention.
In figure: 1- injection needle；2- needle adapter；3- eluate conduit；4- three-way switch valve；5- liquid inlet conduit；6- column
Plug pump；7- liquid impurity filter；8- liquid storage bottle；9- air filtering plug；10-X axis motorized precision translation stage；11-Y axis electric translation
Platform；12-Z axis motorized precision translation stage；13- hand screw；14- needle mount；15- right-angle connector；16- right-angle connector；17-
Connecting plate；18- electric rotary table；19- sample；20- sample stage；21- turntable fixing piece；22- vibration-damped table；23- colored CCD phase
Machine；24- microspur zoom lens；25- camera connecting plate；26- camera support；27- right-angle connector；28- waste collection box；29-
Drop to be arranged；30- hydrophilic region；The micro drop of 31-；The super-hydrophobic region 32-.
Specific embodiment
Below in conjunction with the attached drawing of apparatus of the present invention, the technical solution in apparatus of the present invention is clearly and completely retouched
It states.
First embodiment
The present invention is scarce for arrangement low efficiency, positioning accuracy are poor, arrangement liquid is single present in micro drop arrangement at present etc.
It falls into, provides the extensive micro drop arrangement apparatus and method of a kind of gravity drive, in the parent with wetting sex differernce
Water/super-hydrophobic array structure sample surfaces are accurately arranged micro drop.
It is the schematic diagram of hydrophilic/super-hydrophobic array structure according to the present invention referring to Fig. 3, Fig. 3, while is also the device
Sample (19).The sample (19) is equipped with hydrophilic region (30) and super-hydrophobic region (32) for micro drop of arranging, surface.
For hydrophilic region (30) for carrying liquid to be arranged, which is set as water wetted material or hydrophilic coating.When wait arrange
When the drop of cloth has oil-wet behavior, which is also extended to hydrophobic regions.When liquid i.e. to be arranged is aqueous solution, use is hydrophilic
Material or hydrophilic coating etc. are realized；When liquid to be arranged is oil-based liquid, realized using corresponding lipophilic material or oleophylic coating.
Super-hydrophobic region (32) uses super hydrophobic material or super-hydrophobic coat.Meanwhile when drop to be arranged is oil-based liquid, the area
Domain is extended to superoleophobic material or super oleophobic coating.
When liquid to be arranged flows through hydrophilic region (30), the liquid of designated volume is attracted to hydrophilic region, reservation
Liquid is based on that surface tension of liquid effect is spontaneous to form drop in hydrophilic region, the volume of dropping liquid by the area of hydrophilic region and
Shape accurately determines.When liquid to be arranged flows through super-hydrophobic region (32), no liquid is retained in the region.The sample utilizes
Wetting sex differernce of the liquid to be arranged in hydrophilic region and super-hydrophobic region realizes microtitre arrangement.
Fig. 4 is micro drop arrangement process schematic of the drop by sample surfaces.Micro drop row of the present invention
Cloth method is based on gravity drive.Gravity drive mode is realized by an electric rotary table (18).Original state, such as Fig. 4 (a)
Shown, a hydrophilic region in sample (19) leftmost side injects a large volume of drop to be arranged.Later, utilization is electronic
Turntable (18) tilts sample (19) at the uniform velocity, and sample (19) and horizontal plane form certain angle, and enable each column in array hydrophilic
Region (30) is in same vertical setting of types.Drop to be arranged is acted on by gravity in the component being parallel on clinoplain, there is slide downward
Trend.The gravity suffered by the drop to be arranged is greater than hydrophilic region to drop to be arranged in the component being parallel on clinoplain
When adhesion strength, the quick adipping of drop to be arranged is rolled.The each hydrophilic region (30) for flowing through the perpendicular column can leave one
Quantitative liquid, to realize the purpose of micro drop arrangement in hydrophilic region.
Further, Fig. 3 illustrates hydrophilic/super-hydrophobic array structure of one kind 4 × 4.The array structure can be expanded
Zhan Weigeng big hydrophilic/super-hydrophobic array structure, such as 40 × 40,50 × 100 etc., more massive micro liquid can be achieved in this way
Drop arrangement.Meanwhile line space between hydrophilic/super-hydrophobic array structure and column pitch can pacify and change according to exact arrangement demand
Become；Hydrophilic/super-hydrophobic array structure not only may be configured as the rectangular array of standard, but can be added on the rectangular array of standard or
Remove some hydrophilic regions, or be set as different size of hydrophilic region, becomes derivative array structure.
Further, the accuracy that track is tumbled for raising drop, can further intervene drop trajectories, for example,
Canal shape guiding groove is set in the perpendicular column described in hydrophilic area (30), it is sliding inclined that drop can be effectively prevented.Canal shape guiding groove is cut
Face is designed as rectangle, semicircle or up-side down triangle.Since the structure is relatively simple, even if not providing respective figure, the design
It is easy to be readily appreciated by one skilled in the art.
Further, to improve sampling efficiency, a row can be arranged according to the distribution of hydrophilic area (30) on carrier fluid device
Dropper.At this time, it is only necessary to one of dropper and hydrophilic area (30) align, that is, realize pair of each dropper
Its；Similarly, it is only necessary to enable row's dropper while drop is added dropwise, that is, realize and the quick of gross sample point in array is taken
Sample.
What it was utilized is still basic conception of the invention at this time, falls into protection scope of the present invention, the present invention to this not
It repeats again.
The sample has following advantage:
1) sample substrate selection of the invention is easy.The products such as optional plastic products, glass, ceramics, metal.
2) accurate droplet size control may be implemented in apparatus of the present invention.The volume of arrangement drop depends on hydrophilic region
(30) shapes and sizes etch hydrophilic region (30) by the way that ultraviolet photolithographic technology is high-precision in superhydrophobic substrate.It protects
The shapes and sizes of hydrophilic region have been demonstrate,proved, and then ensure that the volume of arrangement drop, obtain with hydrophilic/super-hydrophobic array structure
Sample.
3) apparatus of the present invention dropping liquid arrangement positioning accuracy is high.Apparatus of the present invention in hydrophilic region and are surpassed using liquid to be arranged
The wetting sex differernce of hydrophobic region realizes microtitre arrangement, is pin-pointed to arrangement position without injection needle, is not injected
Syringe needle position error influences.
4) apparatus of the present invention arrangement is high-efficient, and scalability is strong.The sample of apparatus of the present invention can be by increasing surface area
Large-scale micro drop arrangement is realized with hydrophilic/super-hydrophobic array structure.Meanwhile the present invention can also be on the same sample
Multiple hydrophilic areas (30) being separated from each other are set, is sampled while to realize multiple hydrophilic areas (30), obtains different volumes
Drop.
5) apparatus of the present invention can arrange a variety of different types of liquid simultaneously.The device is by droplet injection system in sample
The one large volume of drop of drop of specific position injection on product (19) surface, it is hydrophilic/super using dropping liquid gravity and sample surfaces later
Hydrophobic array structure double action, realizes the exact placement of micro drop.The dropping liquid type of same perpendicular column arrangement is depended at this
The big drop of perpendicular column injection.By dropping liquid injecting systems, different types of drop can be injected in specially appointed kind progression as needed, in turn
Realize different types of micro drop of arranging.
For the present embodiment in the micro drop of sample (19) surface arrangement, following steps: your relative's water area should be included at least
(32) and after liquid to be arranged contact it separates, keeps the liquid in hydrophilic region (30) and form drop.For the contact side
Formula is directly added dropwise larger volume drop by syringe needle, gravity is recycled to flow down the drop from side and pass through hydrophilic area
Realize the drop arrangement of designated volume in domain (30).
Meanwhile this patent is extended to contact and immersion arrangement mode.Fig. 5 is contact arrangement mode: enabling sample
(19) it moves downward and is come into full contact with the liquid level of drop to be arranged, be slowly withdrawn sample later.Since super-hydrophobic area (32) will not
Liquid is drawn, the liquid after contact will only adhere to hydrophilic area (30), to complete micro drop arrangement.
Further, the liquid container in Fig. 5 can be replaced with spray equipment, directly sprays liquid in special
Hydrophilic/super-hydrophobic array structure sample surfaces formed liquid film, after liquid film leaves super-hydrophobic area (32) due to gravity automatically,
Hydrophilic area (30) will leave sampling liquid.
Fig. 6 is immersion arrangement mode: sample (19) being enabled to be completely immersed in liquid level hereinafter, being slowly withdrawn sample from liquid later
Product.After leaving liquid level due to carrier fluid device, the liquid of super-hydrophobic area (32) can slide automatically, and only hydrophilic area (30) can leave sampling
Liquid, this mode can also realize quick arrangement.
Contact and the purpose of immersion arrangement mode are raising sampling efficiency, are conducive to the serialization to same liquid sample
High-speed sampling.But it is different from second embodiment, mode used by the present embodiment is to change the contact of liquid and carrier fluid device
Mode, to realize the purpose of fast sampling.Because contact and immersion arrangement mode principle are readily appreciated that, operating process letter
It is single, therefore both arrangement apparatus are not explained in detail.
Second embodiment
Fig. 2 gives the sampler based on the design, which includes one with special hydrophilic/super-hydrophobic
The sample of array structure, a dropper and inclination sample table device.In figure, there are special hydrophilic/super-hydrophobic array junctions
The sample (19) of structure, is mounted on sample stage (20).Sample stage (20) connect to form revolute pair with workbench, that is, vibration-damped table (22),
The revolute pair is realized by the electric rotary table (18) in figure.The dropper includes injection needle (1), syringe needle adaptation
Device (2), eluate conduit (3), three-way switch valve (4), liquid inlet conduit (5), plunger pump (6), liquid impurity filter (7), liquid storage
Bottle (8) and air filtering plug (9), for realizing drop control.Wherein injection needle (1), needle adapter (2), eluate conduit
(3), three-way switch valve (4), liquid inlet conduit (5), liquid storage bottle (8) are sequentially connected；The three-way switch valve (4) is gone back and plunger pump
(6), drop control is realized by three-way switch valve (4) and plunger pump (6)；Liquid inlet conduit (5) entrance is inserted into liquid storage bottle (8)
Liquid level hereinafter, inlet be equipped with liquid impurity filter (7)；The liquid storage bottle (8) is equipped with air filtering plug (9).
In addition, to divide into waste collection box (28) in sample stage (20) convenient for devil liquor recovery.For the position for realizing dropper
Control is set, syringe needle positioning control system will be installed on syringe needle (1) additional.To mention high control precision, installs NI Vision Builder for Automated Inspection additional, establish needle
Head (1) is connect with the vision of colorful CCD camera (23), to directly observe syringe needle (1) position by visual computer screen
With micro drop arrangement situation.Each group part is described in further detail below:
Injection needle (1) is used to inject solution to be arranged in sample (19) specific position, passes through needle adapter (2) and out liquid
Conduit (3) connection.Needle adapter (2) is fixed on Z axis motorized precision translation stage (12) by needle mount (14).Syringe needle adaptation
Device (2) is fixed on needle mount (14) by hand screw (13).By D translation platform control needle adapter (2) come
Realize the Accurate Position Control of injection needle (1).Injection needle (1) internal diameter is 100 microns.Eluate conduit (3) one end and syringe needle
Adapter (2) connection, the other end are connected with three-way switch valve (4) left end interface.Three-way switch valve (4) right end interface connects feed liquor
Conduit (5), lower end interface connecting column plug pump (6).Liquid inlet conduit (5) end connects a liquid impurity filter (7), is placed in
Under liquid storage bottle (8) liquid level.Liquid inlet conduit (5) is fixed by the guide hole that liquid storage bottle covers, meanwhile, liquid storage bottle cap top has
One air filtration hole is equipped with air filtering plug (9) in hole.
Syringe needle positioning control system is made of comprising the electric translation of an X-direction a set of three-D electric translation stage
The electricity driving displacement platform (12) of platform (10), the motorized precision translation stage (11) of Y direction and a Z-direction.Wherein X-axis is electronic
Displacement platform (11) is mounted on vibration-damped table (22).X, two motorized precision translation stages of Y-axis are connected using connecting plate (17)；Y, Z axis two
A motorized precision translation stage is connected using right-angle connector (16)；Z axis motorized precision translation stage (12) uses right-angle connector (15) and syringe needle
Fixing piece (14) is connected.Needle mount (14) passes through manual bolt (13) fixed needle adapter (2).Needle adapter (2)
Lower end is installed by injection needle (1).Three motorized precision translation stages (10) (11) (12) on X, Y, Z axis direction pass through control syringe needle adaptation
Device (2) controls the predeterminated position on injection needle (1) precise motion to sample (19).
Sample (19) is placed on sample stage (20), is fixed using double faced adhesive tape.Sample stage (20) and electric rotary table
(18) it connects.Electric rotary table (18) is connect by turntable fixing piece (21) with vibration-damped table (22).Electric rotary table (18) is used
In inclination sample (19), sample holds drop to be arranged on (19).Original state makes sample (19) by electric rotary table (18)
Keep horizontal positioned.Electric rotary table (18) is for tilting sample (19) at a high speed.When having drop to be arranged on sample (19), to
Arrangement drop is by gravity in the component effect being parallel on clinoplain, adipping movement.Drop passes through sample (19)
When special hydrophilic/super-hydrophobic array structure, super-hydrophobic region does not have drop residual, and hydrophilic region maintains the liquid of particular size
Drop.Using special hydrophilic/super-hydrophobic array structure double action of drop gravity and sample, the accurate of micro drop is realized
Arrangement.Arrangement process is completed, and remaining drop rolls down in waste collection box (28).
The device further includes an easy NI Vision Builder for Automated Inspection.It passes through colorful CCD camera (23) observing samples
(19) drop arranged on.Colorful CCD camera (23) is furnished with a microspur zoom lens (24).Colorful CCD camera (23) passes through
Camera connecting plate (25) is fixed on camera support (26).Camera support (26) is fixed on vibration-damped table by right-angle connector (27)
(22) on.The input terminal of the colorful CCD camera (23) and computer is electrically connected, and the picture signal for that will shoot is on computers
Display.
The work flow diagram of the drop arrangement apparatus, as shown in Figure 1.Tri- axis electricity driving displacement platform of XYZ, electric rotary table
(18), plunger pump (6), colorful CCD camera (23) are uniformly controlled by computer, according to the program write, are carried out by step.Its
In, tri- axis electricity driving displacement platform of XYZ is used to control the three-dimensional motion of injection needle (1)；Electric rotary table (18) is for controlling sample
The tilt angle of sample platform (20)；Plunger pump (6) realizes the drop of injection needle (1) injection particular size volume；Colorful CCD camera
(23) drop for arranging on observing samples (19).To realize the extensive micro drop arrangement of high-precision, automation.
The arrangement method of the present embodiment device is described below:
1) drop arrangement apparatus various components, connecting conduit and syringe needle are installed.
2) XYZ three-D electric displacement platform is used, mobile injection needle (1) arrives initial default location.
3) sample (19) is placed on sample stage (20) surface, is fixed by double faced adhesive tape.
4) switching three-way switching valve (4) is extremely: plunger pump (6) and liquid inlet conduit (5) connected state.Plunger pump (6) starts work
Make, is drawn inside experimental liquid to Plunger pump body from liquid storage bottle (8).
5) switching three-way switching valve (4) is extremely: plunger pump (6) and eluate conduit (3) connected state.Plunger pump (6) work,
Liquid conveying in plunger pump body is to injection needle (1).
6) above-mentioned two steps operation is repeated, discharge liquid inlet conduit (5), three-way switch valve (4), plunger pump (6), liquid is led out
Air in pipe (3), needle adapter (2) and injection needle (1).The liquid of injection needle (1) discharge is dropped in waste collection
In box (28).
7) sample (19) keeps horizontality by sample stage (20).Motorized precision translation stage controls injection needle (1) and accurately transports
It moves to operating position.Plunger pump (6) work, successively predeterminated position on sample (19) inject one group of 0.15ml size wait arrange
Cloth drop (29).
8) electric rotary table (18) moves, and tilts sample (19) towards specific direction.When tilt angle is greater than sample
(19) when keeping the critical value of drop, drop is because gravity is acted in the component being parallel in angled section, quickly to inclination side
To sliding.When drop is by the special hydrophilic/super-hydrophobic array structure of sample, super-hydrophobic region (32) does not keep drop, and
The micro drop (31) of a certain size volume is maintained in hydrophilic region (30), volume size passes through the hydrophilic array of sample surfaces
The shapes and sizes of structure determine.Special hydrophilic/super-hydrophobic array structure using drop gravity and sample (19) is dual
Effect is realized and is arranged in the micro drop of sample (19) surface hydrophilic region high-precision automatic.
Claims (10)
1. a kind of extensive micro drop arrangement apparatus of gravity drive, which is characterized in that the device is hydrophilic/super thin with one
The sample of water array structure；The sample is arranged into array structure by hydrophilic region and super-hydrophobic region, and hydrophilic region is super-hydrophobic
Region surrounds completely；The sample is rectangle, etches hydrophilic area by the way that ultraviolet photolithographic technology is high-precision in superhydrophobic substrate
Domain.
2. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 1, which is characterized in that hydrophilic
Region uses water wetted material or hydrophilic coating；Super-hydrophobic region uses super hydrophobic material or super-hydrophobic coat.
3. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 1, which is characterized in that parent
The line space and column pitch of water/super-hydrophobic array structure hydrophilic region can pacify to be configured according to exact arrangement drop demand；It should
Hydrophilic/super-hydrophobic array structure may be configured as the rectangular array of standard, can also be added or removed from one on the rectangular array of standard
A little hydrophilic regions, or it is set as different size of hydrophilic region, become derivative array structure.
4. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 1, which is characterized in that including
One sample, dropping liquid injecting systems, kinetic control system, NI Vision Builder for Automated Inspection with special hydrophilic/super-hydrophobic array structure
And computer control system；
Wherein, dropping liquid injecting systems include: injection needle, are connected by needle adapter with eluate conduit；Eluate conduit it is another
One end is connected with three-way switch valve left end interface；Three-way switch valve right end interface is connected with liquid inlet conduit, lower end interface and plunger
Pump is connected；Liquid inlet conduit end connects a liquid impurity filter, for filtering the impurity in liquid storage bottle liquid；Liquid inlet conduit
It is fixed by the guide hole that liquid storage bottle covers；Liquid storage bottle cap is equipped with air filtering plug there are one air filtration hole in hole；
Kinetic control system includes two parts of syringe needle positioning system and sample inclination system；Syringe needle positioning system includes X-axis electricity
Dynamic translation stage, Y-axis motorized precision translation stage and Z axis motorized precision translation stage；X-axis motorized precision translation stage, Y-axis motorized precision translation stage and Z axis are electronic flat
Moving stage is connected two-by-two, for realizing the space orientation of syringe needle；Needle mount is connected to Z axis electric translation by right-angle connector
On platform, needle adapter is fixed in needle mount by hand screw, and injection needle is connected with needle adapter；Y-axis electricity
Dynamic translation stage is connected with Z axis motorized precision translation stage by right-angle connector；X-axis motorized precision translation stage and Y-axis motorized precision translation stage pass through company
Fishplate bar is connected；
Electric rotary table is for controlling sample inclination special angle；Sample is placed on sample stage；Sample stage and electric rotary table
Connection, electric rotary table are fixed in vibration-damped table by turntable fixing piece；Using drop gravity and sample it is special it is hydrophilic/
Super-hydrophobic array structure realizes the exact placement of micro drop；Initial stage, sample keep horizontal positioned.
5. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 4, which is characterized in that in parent
It is erected locating for water/super-hydrophobic array structure hydrophilic region and canal shape guiding groove is set in column；The section of canal shape guiding groove is rectangle, half
Round or up-side down triangle.
6. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 4, which is characterized in that dropping liquid
Injecting systems include two or more injection needles, the hydrophilic region distribution of each injection needle and hydrophilic/super-hydrophobic array structure
It is corresponding.
7. a kind of extensive micro drop arrangement apparatus of gravity drive according to claim 1, which is characterized in that this is micro-
Amount drop arrangement apparatus is extended to contact and immersion arrangement mode；Contact arrangement mode enables sample drop to be arranged
Liquid level is slowly withdrawn sample after coming into full contact with；Immersion arrangement mode enables sample be completely immersed in liquid level hereinafter, later from liquid
In be slowly withdrawn sample.
8. a kind of described in any item drop arrangement sides of the extensive micro drop arrangement apparatus of gravity drive claim 1-7
Method, which comprises the following steps: separated after enabling the hydrophilic region of carrier fluid device contact with liquid, make liquid hydrophilic
Region forms drop.
9. a kind of drop arrangement method of the extensive micro drop arrangement apparatus of gravity drive according to claim 8,
It is characterized in that, sample is enabled to tilt with horizontal plane, dropping liquid injecting systems are by droplet injection in a certain hydrophilic/super-hydrophobic array junctions
Column present position is erected locating for the hydrophilic region of structure, so that drop tumbles naturally along the column.
10. a kind of drop arrangement method of the extensive micro drop arrangement apparatus of gravity drive according to claim 8,
It is characterized by comprising the following steps:
Dropping liquid injecting systems, which are aligned, erects column locating for hydrophilic region；
The drop of dropping liquid injecting systems control predefined size, which is fallen into, erects column locating for hydrophilic region；
Drop erects column locating for the hydrophilic region and flows naturally, contacts with hydrophilic region and leaves liquid in hydrophilic region, to take
The sample of liquid must be loaded with.
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| CN111420613A (en) * | 2020-05-07 | 2020-07-17 | 桂林电子科技大学 | Device and method for preparing boxed liquid marble |
| CN112827533A (en) * | 2021-01-08 | 2021-05-25 | 青岛大学 | Desktop type micro-droplet chemical reaction experiment platform based on super-hydrophobic material |
| CN113713868A (en) * | 2021-09-13 | 2021-11-30 | 北京京东方技术开发有限公司 | Microfluidic chip and manufacturing method thereof |
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| CN110354927B (en) | 2021-12-21 |
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