CN110339085A - A kind of composition and its application containing ceramide liposome - Google Patents
A kind of composition and its application containing ceramide liposome Download PDFInfo
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- CN110339085A CN110339085A CN201910125064.7A CN201910125064A CN110339085A CN 110339085 A CN110339085 A CN 110339085A CN 201910125064 A CN201910125064 A CN 201910125064A CN 110339085 A CN110339085 A CN 110339085A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0295—Liquid crystals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/342—Alcohols having more than seven atoms in an unbroken chain
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of composition containing ceramide liposome and its applications, it is intended to provide a kind of with liquid crystal structure, uniform particle diameter, good water solubility, and the higher liposome composition of stability, be conducive to ceramide and cuticula is directly acted on through skin surface by liposome structure, skin barrier is repaired, is increased operation rate；Technical points are that the composition containing ceramide liposome mainly includes following component: 0.1-10% ceramide, 1-5% caprylic/capric triglyceride, 5-20% cetostearyl alcohol, 1-4% emulsifier count by weight percentage；The application that the invention also discloses the compositions in cosmetics, has a good application prospect；Belong to cosmetic technical field.
Description
Technical field
The invention discloses a kind of compositions of liposome, specifically, being a kind of combination containing ceramide liposome
Object, and the liposome is in liquid crystal structure, the invention also discloses application of the liposome composition in cosmetics, belong to makeup
Product technical field.
Background technique
Liposome is to be suspended to assemble in aqueous solution by phospholipid molecule to be formed, and form the spherical phospholipid of liposome vesicle
Molecule is in dual layer arrangement, it is similar to skin keratin bilayer.And state in which is different at different temperatures for phosphatide, with
The raising of temperature, when reaching phase transition temperature, most phosphatide can be changed into liquid crystal state from colloidal state, when liposome is in colloidal state, rouge
The free volume of matter bilayer is reduced, and permeability will substantially reduce, and when being in liquid crystal state, the permeability of lipid film can be significant
Increase, effective component can be improved and promote effective component infiltration in the permeability of skin keratin, and directly act on skin angle
Matter layer improves utilization rate of active components.
For the material of liquid crystalline state because its special microstructure is other than having mobility and anisotropy concurrently, there are also enhancings to stablize
Property, the unique property of control active material slow release etc..Therefore, the liquid crystal state liposome for wrapping up effective component is applied to change
In cosmetic, effective component can promote through epidermis, and generate enough accumulation in corresponding site, to reach efficient moisture-retention lock
Water, reparation skin, anti-oxidant, anti-aging and other effects, and have good compatibility to skin, excellent skin can be provided to skin
Sense.
Ceramide be lipoid between being present in the outermost corneocyte of human skin main composition (>
50%), it can not only assist in skin and lock moisture, carry protection skin in the barrier of skin surface one layer of waterproof of formation
With moisten, moisturizing the effects of, moreover it is possible to promote skin barrier self-regeneration, protect the skin from the functions such as environmental stimuli, but skin
The secretion of middle ceramide can be reduced with advancing age, and then cause dry skin, aging, coarse etc. are a series of to ask
Topic.
Further investigation with people to ceramide, more and more scientific research institutions, brand company, which are proposed, largely to be contained
The cosmetics of ceramide cause the extensive concern of people, and become hot-sale products.However ceramide fusing point is higher, molten
Xie Du is lower, and is easy to crystallize or flocculate from cosmetic formulations, not only influences formulation stability, also reduces ceramide
Utilization rate, greatly limit their application.
Summary of the invention
In order to solve the disadvantage that the prior art and shortcoming, the object of the present invention is to provide one kind to contain ceramide lipid
The composition of body, the liposome prepared through the invention have liquid crystal structure, and not only quantity is more, uniform particle diameter, but also water-soluble
It is good, stablize, so that overcoming ceramide is easy to appear crystallization, flocculation, and the problem that solubility is lower, can preferably apply
Into cosmetic formulations.
First technical solution provided by the invention is such that
A kind of composition containing ceramide liposome, the composition are in liquid crystal structure, and the composition mainly includes oil
Phase and water phase, with the total weight of the composition, the component and its content that the oil mutually includes with the water phase are as follows:
Oil mutually includes:
Ceramide: 0.1-10%, caprylic/capric triglyceride: 1-5%, cetostearyl alcohol: 5-20%.
Water phase includes:
Emulsifier: 1-4%, surplus are water.
The preferred usage amount of ceramide used in the present invention is 0.5-10%.
The preferred usage amount of caprylic/capric triglyceride used in the present invention is 3-5%.
The preferred usage amount of cetostearyl alcohol used in the present invention is 5-10%.
The preferred usage amount of emulsifier used in the present invention is 2-3%.
The above-mentioned composition containing ceramide liposome, fatty alcohol is also contained in oily phase, and the fatty alcohol weight ratio is group
The 0.1-1.5% of object total weight is closed, at least one of cetanol, octadecyl alcolol, docosanol are selected from.
The above-mentioned composition containing ceramide liposome, phosphatide is also contained in water phase, and the phosphatide weight ratio is composition
The 0.1-3% of total weight is selected from lecithin, hydrolecithin, soybean lecithin, phosphatidyl-ethanolamine, distearoylphosphatidyl gallbladder
At least one of alkali.
The above-mentioned composition containing ceramide liposome, thickener is also contained in water phase, and the thickener weight ratio is group
The 0.01-0.5% for closing object total weight, in carbomer, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer
At least one.
The above-mentioned composition containing ceramide liposome, also includes preservative, and the preservative weight ratio is that composition is total
The 0.5-1.1% of weight is selected from 1,2- hexylene glycol, parahydroxyacet-ophenone, Bis(hydroxymethyl)imidazolidinyl urea, Nipasol, hydroxyl
At least one of benzene methyl, o- cymene -5- alcohol, Chlorphenesin, Phenoxyethanol, Sensiva SC50.
The above-mentioned composition containing ceramide liposome, also contains neutralizer, and the neutralizer weight ratio is that composition is total
The 0.01-0.5% of weight is selected from least one of arginine, sodium hydroxide, potassium hydroxide, triethanolamine.
Above-mentioned composition, the ceramide are ceramide II, ceramide III, at least one in ceramide VI
Kind.
Above-mentioned composition, the emulsifier are two (lauramide glutamine) Sodium lysinates, stearine, PEG-
At least one of the smooth stearate of 100 stearates, sorb, polysorbate -60.
Second technical solution provided by the invention is: a kind of side preparing the above-mentioned composition containing ceramide liposome
Method, its step are as follows:
1) 85-90 DEG C mutually is heated separately to water phase by oily；
2) oil is mutually quickly poured into water phase, homogeneous 3min in the case where homogeneous speed is 13000rpm；
3) it is cooled to room temperature, ceramide liposome can be prepared.
Third technical solution provided by the invention is the composition containing ceramide liposome as cosmetic additive agent
Application, wherein the cosmetics be lotion, cream, toner, essence, facial mask, make-up remover, gel of washing one's face wherein it
One.
Compared with prior art, technical solution provided by the invention has following technological merit:
1) ceramide liposome provided by the invention is white creams, and free from extraneous odour, the average grain diameter of liposome is 2.5 μ
m.By invented liposomes at 45 DEG C of high temperature, -15 DEG C of low temperature, -15 DEG C of 45 DEG C of high temperature, 25 DEG C of room temperature and low temperature so circulation are repeatedly
Under the conditions of and natural lighting under the conditions of place and do not occur within 90 days layering, particle is precipitated, is coarse, becomes saturating phenomenon, also do not go out
Now other abnormal phenomenon, the liposome stability such as discoloration, spoiled are fine.
2) liposome provided by the invention is a kind of liquid crystal state structure, due to the pure and mild caprylic/capric glycerol three of cetearyl alcohol
The addition of ester, it is the liquid crystal state liposome formed in system more (see Fig. 2-4), more stable.Because ceramide molecule is by two
The fatty acid alkyl chain of different carbon chain, two hydroxyls and amide groups composition, have bimolecular structure, make it both and have and is hydrophilic
Property there is lipophilicity again, but there is no emulsifiability, by suitable lipid solubilization, the special construction of cetostearyl alcohol be can be plugged into
In ceramide molecule gap, suitable emulsifier is added, so that forming the viscoplasticity interface of dense regular at oil/water interface
Film, facilitates the formation of liquid crystal structure, and the addition of liquid fat caprylic/capric triglyceride, makes the emulsification grain of whole system
Son is gradually varied to liquidus behavior from solid-state properties, forms more liquid crystal structures, and reduces ceramide in system
Fusing point prevents ceramide from crystallizing and is precipitated, improves the stability of entire liquid crystal structure.
3) method of preparation composition containing ceramide liposome provided by the invention, can obtain with liquid crystal structure, grain
Diameter is uniform, good water solubility, and the higher liposome composition of stability, so that it is saturating by liposome structure to be conducive to ceramide
It crosses skin surface and directly acts on keratoderma, repair skin barrier, increase operation rate.
4) composition provided by the invention can be compounded arbitrarily with cosmetic formulations under normal temperature conditions, to reach efficient guarantor
Wet water lock, reparation skin, anti-oxidant, anti-aging and other effects, and have good compatibility to skin, it can be provided to skin excellent
Skin sense, have a good application prospect.
Detailed description of the invention
Fig. 1 is the grain size distribution of the composition containing ceramide liposome prepared by embodiment 19；
Fig. 2 is the petrographic microscope figure of the composition containing ceramide liposome prepared by embodiment 10；
Fig. 3 is the petrographic microscope figure of the composition containing ceramide liposome prepared by comparative example 5；
Fig. 4 is the petrographic microscope figure of the composition containing ceramide liposome prepared by comparative example 6；
Fig. 5 is cosmetics TEWL recovery rate comparison diagram prepared by embodiment 15,17,19 and comparative example 1,2.
Specific embodiment
With reference to embodiment, claim of the invention is described in further detail, but do not constituted pair
Any restrictions of the invention, the modification of anyone limited times made in the claims in the present invention protection scope, still in this hair
Within bright claims.For not specifically specified technological parameter or condition, routine techniques progress can refer to.
It should be noted that raw material employed in technical solution provided by the invention, unless otherwise specified, by normal
The preparation of rule means is bought by commercial channel.
Embodiment 1
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, surplus is water.
Embodiment 2
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.5%, caprylic/capric triglyceride: 3%, cetostearyl alcohol: 5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 2%, surplus is water.
Embodiment 3
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 8%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 2.5%, surplus is water.
Embodiment 4
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 10%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, surplus is water.
Embodiment 5
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, surplus is water.
Embodiment 6
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%, cetanol: 0.1%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, surplus is water.
Embodiment 7
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, surplus is water.
Embodiment 8
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%, cetanol: 1.5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, surplus is water.
Embodiment 9
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%, cetanol: 0.1%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, hydrolecithin: 0.1%, surplus is water.
Embodiment 10
A kind of composition containing ceramide liposome, this contains the petrographic microscope figure ginseng of ceramide liposome composition
Read Fig. 2；It is composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, surplus is water.
Embodiment 11
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%, cetanol: 1.5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, hydrolecithin: 3%, surplus is water.
Embodiment 12
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%, cetanol: 0.1%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, hydrolecithin: 0.1%, carbomer: 0.01%, surplus
For water.
Embodiment 13
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, carbomer: 0.3%, surplus is
Water.
Embodiment 14
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%, cetanol: 1.5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, hydrolecithin: 3%, carbomer: 0.5%, surplus is
Water.
Embodiment 15
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%, cetanol: 0.1%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, hydrolecithin: 0.1%, carbomer: 0.01%, surplus
For water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.5%.
Embodiment 16
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, carbomer: 0.3%, surplus is
Water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.8%.
Embodiment 17
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%, cetanol: 1.5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, hydrolecithin: 3%, carbomer: 0.5%, surplus is
Water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 1.1%.
Embodiment 18
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 0.1%, caprylic/capric triglyceride: 1%, cetostearyl alcohol: 5%, cetanol: 0.1%.
Water phase:
Two (lauramide glutamine) Sodium lysinates: 1%, hydrolecithin: 0.1%, carbomer: 0.01%, surplus
For water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.5%.
Neutralizer: arginine: 0.01%.
Embodiment 19
A kind of composition containing ceramide liposome, this contain the grain size distribution of ceramide liposome composition refering to
Fig. 1；It is composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, carbomer: 0.3%, surplus is
Water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.8%.
Neutralizer: arginine: 0.3%.
Embodiment 20
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 10%, caprylic/capric triglyceride: 5%, cetostearyl alcohol: 20%, cetanol: 1.5%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 4%, hydrolecithin: 3%, carbomer: 0.5%, surplus is
Water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 1.1%.
Neutralizer: arginine: 0.5%.
Embodiment 21
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide III: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, octadecyl alcolol: 1%.
Water phase includes:
Stearine //PEG-100 stearate: 3%, lecithin: 1.5%, acrylic acid (ester) class/C10-30 alkane
Alcohol acrylate crosspolymers: 0.3%, surplus is water.
Preservative: Chlorphenesin and Phenoxyethanol // Sensiva SC50: 0.8%
Neutralizer: triethanolamine: 0.3%.
Embodiment 22
A kind of composition containing ceramide liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide VI: 5%, caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%, docosanol: 1%.
Water phase includes:
The smooth stearate of sorb and polysorbate -60:3%, soybean lecithin: 1.5%, carbomer: 0.3%, surplus is
Water.
Preservative: parahydroxyacet-ophenone and Bis(hydroxymethyl)imidazolidinyl urea: 0.6%
Neutralizer: sodium hydroxide: 0.3%.
In order to prove that effect of the invention, the application are also provided with following comparative example, with embodiment provided by the present application into
Row Experimental comparison:
Comparative example 1
Comparative example 1 provides a kind of composition of liposome, composed of the following components with the total weight of the composition:
Oily phase:
Caprylic/capric triglyceride: 4%, cetostearyl alcohol: 10%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, surplus is water.
Comparative example 2
Comparative example 2 provides a kind of composition of liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, cetostearyl alcohol: 10%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, surplus is water.
Comparative example 3
Comparative example 3 provides a kind of composition of liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, surplus is water.
Comparative example 4
Comparative example 4 provides a kind of composition of liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetanol: 1%.
Water phase includes:
Stearine //PEG-100 stearate: 3%, carbomer: 0.3%, surplus is water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.8%.
Neutralizer: arginine: 0.3%.
Comparative example 5
Comparative example 5 provides a kind of composition of liposome, and the petrographic microscope figure of the composition is refering to Fig. 4；
It is composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, caprylic/capric triglyceride: 4%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, surplus is water.
Comparative example 6
Comparative example 6 provides a kind of composition of liposome, and the petrographic microscope figure of the composition is refering to Fig. 4；
It is composed of the following components with the total weight of the composition:
Oily phase:
Ceramide II: 5%, cetostearyl alcohol: 10%, cetanol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, hydrolecithin: 1.5%, surplus is water.
Comparative example 7
Comparative example 7 provides a kind of composition of liposome, composed of the following components with the total weight of the composition:
Oily phase:
Ceramide III: 5%, cetostearyl alcohol: 10%, octadecyl alcolol: 1%.
Water phase includes:
Two (lauramide glutamine) Sodium lysinates: 3%, carbomer: 0.3%, surplus is water.
Preservative: 1,2- hexylene glycol and parahydroxyacet-ophenone: 0.8%.
Neutralizer: triethanolamine: 0.3%.
Composition described in 1-22 of the embodiment of the present invention and comparative example 1-7 the preparation method is as follows:
1) 85-90 DEG C is mutually heated separately to water phase (if prevented in water phase components containing preservative and neutralizer by oily
Rotten agent and neutralizer are without heating)；
2) oil is mutually quickly poured into water phase, homogeneous 3min in the case where homogeneous speed is 13000rpm；
3) 70 DEG C of addition preservatives and neutralizer are cooled under agitation (if preservative free and neutralization in formula
Agent is then omitted), it is cooled to room temperature, ceramide liposome can be prepared.
Composition prepared by the present invention containing ceramide liposome can be used as cosmetic additive agent and be added to various dosage forms
Cosmetics in, such as lotion, cream, toner, essence, facial mask, make-up remover, gel of washing one's face, liposome composition making up
Additive amount in product is the 0.1-10% of gross mass.
A kind of preparation of the cosmetics containing ceramide liposome:
Appropriate pure water is added to be heated to 30- 1-22 of the embodiment of the present invention and comparative example 1-7 ceramide liposome prepared
35 DEG C are dispersed with stirring uniformly, and room temperature is added in the common cosmetic formulations system of any commercial available, is stirred evenly, wherein liposome
Composition accounts for the 5% of cosmetics gross mass to get the cosmetics containing ceramide liposome.
Cosmetics containing ceramide liposome provided by the invention are given below in order to better illustrate the present invention the advantages of
Stability test and efficacy test:
1, stability test
1) test sample: contain ceramide liposome by what embodiment 1-22, comparative example 1-7 liposome composition were prepared
Cosmetics.
2) test method: being packed into test tube for test sample, and 8 groups of experiments are respectively set in each sample, take two groups in high temperature 45
It is continuously placed at DEG C 90 days；Two groups are taken continuously to place at -15 DEG C of low temperature 90 days；Two groups are taken successively to place 1 at 45 DEG C of high temperature
It, place 1 day and -15 DEG C of low temperature at 25 DEG C of room temperature at place 1 day, and under the conditions of so recycling repeatedly and (hereinafter abbreviated as intersecting)
It places 90 days；Last two groups are continuously placed 90 days under the conditions of natural lighting, then observe each group sample whether have layering, crystallization,
Phenomena such as changing colour, be spoiled.
From test result as can be seen that ceramide liposome provided by the invention preparation cosmetics, 45 DEG C of high temperature,
It is placed under -15 DEG C of low temperature, crossing condition and under the conditions of natural lighting and does not occur within 90 days layering, particle is precipitated, is coarse, becomes
Saturating phenomenon does not also occur other abnormal phenomenon such as discoloration, spoiled.Do not add ceramide that can also keep good in comparative example 1
Stability, but comparative example 2-7 occur different degrees of crystallization, layering, discoloration, it is spoiled phenomena such as, illustrate ceramide in height
Easily demulsification crystallization, causes system unstable under temperature or cryogenic conditions, and in the pure and mild caprylic/capric triglyceride body of cetearyl alcohol
In system, ceramide does not occur any abnormal phenomenon, it is known that liposome stability prepared by the present invention is fine.
2, skin irritation is tested
1) test sample: the cosmetics containing ceramide liposome prepared by embodiment 1-22 liposome composition.
2) test method: choosing 110 volunteers, wherein female 70, and male 40 is randomly divided into 22 groups (every group 5), by
Examination person, which should meet, aspiration inclusion criteria, no allergic dermatitis history, and the previous moon is also not used excitant product.
It is small that cosmetics about 0.020~0.025ml of Example 1-22 preparation is applied to spot tester (Beijing science popularization instrument plant)
In room, external application special adhesive tape is pasted on subject back, removes spot tester after 24 hours, if any the wet paper handkerchief of surplus products
It gently wipes, respectively at 0.5 hour, 24 hours, 48 hours, by dermoreaction point in " cosmetics safety technical specification " (2015)
Grade standard records its result.Standard of perfection is shown in Table 1.
1 standard of perfection of table
| Grade | Standard of perfection |
| 0 | It is non-stimulated, without erythema |
| 1 | Slight erythema |
| 2 | Erythema |
| 3 | Erythema, papule, bubble |
| 4 | Serious attached edema, bulla |
3) enclosed type patch test result: 110 subjects were at 0.5 hour, 24 hours, and 48 hours to embodiment 1-22 system
The cosmetics containing ceramide liposome obtained do not show any adverse reaction, the diseases such as erythema, burn feeling, itch do not occur
Shape.Illustrate that the composition of the invention containing ceramide liposome is non-stimulated to skin.
3, moisture-keeping efficacy is tested
1) test sample: contain ceramide liposome by what embodiment 1-22, comparative example 1-7 liposome composition were prepared
Cosmetics.
2) test philosophy: the moisture content using capacitance method measurement human skin keratinocyte's layer is based on water and other substances
Difference in dielectric constant is significant, and according to the difference of keratoderma moisture content, the skin pricktest capacitance measured is different, and parameter can generation
Table skin moisture content.
3) volunteer 145 that average age is 25~45 years old test method: are chosen, wherein female volunteers 90, male
Property volunteer 55, be randomly divided into 29 groups, every group 5, subjects skin's health meets inclusion criteria.Before subject's right hand
It draws on the inside of arm as 5 × 5cm2Test area, test specimen amount be 1g, use moisture content of skin tester Corneometer
CM825 is tested respectively using before cosmetics, and using the water content of skin after 6 hours, is repeated 5 times respectively, is obtained average value,
It calculates water content and increases percentage.During experiment, subject cannot smear any other cosmetics in Test sites.
Skin moisture content Growth Rate Calculation formula is as follows:
Skin moisture content growth rate %=(MMV6-MMV0)/MMV0× 100%；
MMV0: use preceding moisture content of skin；
MMV6: use moisture content of skin after 6 hours.
Skin moisture content growth rate measurement result is shown in Table 2.
2 skin moisture content growth rate result of table
Can be seen that cosmetics prepared by embodiment 1-22 from the experimental result of table 2 can significantly improve skin moisture content,
And after using 6h, skin moisture content growth rate is still up to 60% or more, illustrates to add ceramide liposome of the present invention
The cosmetics of composition have effects that good moisturizing water lock, and wherein the cosmetics moistening effect of embodiment 17-22 preparation is best.
And after the cosmetics prepared using comparative example 1-7, moisture content growth rate is between 19-28%, than making up made from embodiment
Product moisture-retaining capacity wants poor.
3, water loss amount (TEWL) measures
1) test sample: by 1,2,3,4,6 rouge of embodiment 1,3,5,7,9,11,13,15,17,19,21 and comparative example
The cosmetics containing ceramide liposome that liposomal composition is prepared.
2) test philosophy: skin water loss amount TEWL is the important parameter for assessing moisture of skin protection layer function, can be used
To evaluate the power of skin barrier function.Protective layer of skin is better, and moisture content is higher, the numerical value of skin water loss TEWL
It is lower, the unit of TEWL are as follows: g/hm2。
3) volunteer 80 that average age is 25~45 years old test method: are chosen, wherein female volunteers 50, male
Property volunteer 30, be randomly divided into 16 groups, every group 5, subjects skin's health meets inclusion criteria.Before subject's right hand
It draws on the inside of arm as 5 × 5cm2Experimental Area, use the barrier function of adhesive tape stripping method failure test area skin, test block
For the skin in domain with laboratory sample is smeared after tape pull five times, sample usage amount is 1g, then with Tewameter TM300 points
Not Ce Shi skin be destroyed before, be destroyed after, and smear sample 0.5h, 1h, 1.5h and 2h after moisture of skin number of dropouts, point
It is not repeated 5 times, obtains average value.During experiment, subject cannot smear any other cosmetics in Test sites.
The TEWL value before skin is destroyed is tested, is indicated with A, is calculated as recovery rate 100%；Skin measures at once after being destroyed
Its TEWL value, is indicated with B, is calculated as recovery rate 0%；TEWL value after when last test smearing sample t, is indicated with C, is thus calculated
The recovery rate of human skin barrier function.
TEWL recovery rate calculation formula is as follows:
TEWL recovery rate %=(B-A)/(C-A) × 100%；
TEWL recovery rate measurement result is shown in Table 3.
3 human skin barrier function recovery rate result of table
It can be seen that the cosmetics 1.5h that embodiment is smeared by adhesive tape stripping damage skin from the experimental result of table 3
Afterwards, human skin barrier function recovery rate reaches 83% or more, and wherein the cosmetics recovery rate of embodiment 17 reaches most preferably, and applies
After the cosmetics 1.5h for smearing comparative example, human skin barrier function recovery rate only has 60% or so.And with the extension of time,
Faster, TEWL recovery rate comparison diagram illustrates system of the present invention refering to Fig. 5 to the TEWL recovery rate decrease speed of smearing comparative example cosmetics
Standby liposome is bigger to the TEWL value recovery extent of skin, is more advantageous to the self-regeneration of skin barrier.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of composition containing ceramide liposome, which is characterized in that the composition is in liquid crystal structure, the composition master
Will be comprising oil phase and water phase, with the total weight of the composition, component and its content that the oil phase and the water phase include
It is as follows:
Ceramide: 0.1-10%,
Caprylic/capric triglyceride: 1-5%,
Cetostearyl alcohol: 5-20%；
Water phase includes:
Emulsifier: 1-4%,
Surplus is water.
2. the composition according to claim 1 containing ceramide liposome, which is characterized in that with the total of the composition
Poidometer, also contains 0.1-1.5% fatty alcohol in the oily phase, and the fatty alcohol is cetanol, octadecyl alcolol, in docosanol
At least one.
3. the composition according to claim 1 containing ceramide liposome, which is characterized in that with the total of the composition
Poidometer, also contains 0.1-3% phosphatide in the water phase, the phosphatide be lecithin, hydrolecithin, soybean lecithin,
At least one of phosphatidyl-ethanolamine, Distearoyl Phosphatidylcholine.
4. the composition according to claim 1 containing ceramide liposome, which is characterized in that with the total of the composition
Poidometer, also contains 0.01-0.5% thickener in the water phase, the thickener be carbomer, acrylic acid (ester) class/
At least one of C10-30 alkylol acrylamide acid esters cross-linked polymer.
5. the composition according to claim 1 containing ceramide liposome, which is characterized in that with the total of the composition
Poidometer, 0.5-1.1% preservative is also contained in the composition water phase, and the preservative is 1,2- hexylene glycol, to hydroxyl
Acetophenone, Bis(hydroxymethyl)imidazolidinyl urea, Nipasol, methyl hydroxybenzoate, o- cymene -5- alcohol, Chlorphenesin, benzene oxygen second
At least one of alcohol, Sensiva SC50.
6. the composition according to claim 1 containing ceramide liposome, which is characterized in that with the total of the composition
Poidometer, also contains neutralizer 0.01-0.5% in the composition water phase, the neutralizer be arginine, sodium hydroxide,
At least one of potassium hydroxide, triethanolamine.
7. the composition according to claim 1 containing ceramide liposome, which is characterized in that the ceramide is mind
Through at least one of amide II, ceramide III, ceramide VI.
8. the composition according to claim 1 containing ceramide liposome, which is characterized in that the emulsifier is two
(lauramide glutamine) Sodium lysinate, stearine, PEG-100 stearate, the smooth stearate of sorb, poly- sorb
At least one of alcohol ester -60.
9. the preparation method of the composition described in claim 1 containing ceramide liposome, which is characterized in that under mainly including
State step:
1) 85-90 DEG C mutually is heated separately to water phase by oily；
2) oil is mutually quickly poured into water phase, homogeneous 3min in the case where homogeneous speed is 13000rpm；
3) it is cooled to room temperature, ceramide liposome can be prepared.
10. application of any composition containing ceramide liposome of claim 1~8 as cosmetic additive agent.
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| CN111568829A (en) * | 2020-06-03 | 2020-08-25 | 上海新高姿化妆品有限公司 | High-content ceramide repair cream and preparation method thereof |
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| CN112206184A (en) * | 2020-11-02 | 2021-01-12 | 南京飞天一淼科技服务有限公司 | Anti-wrinkle repair type eye cream and preparation method thereof |
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| CN112870151A (en) * | 2021-02-08 | 2021-06-01 | 武汉骏安生物科技有限公司 | Skin barrier repair composition containing sodium polyglutamate and application thereof |
| CN113350311A (en) * | 2021-06-23 | 2021-09-07 | 浙江宜格企业管理集团有限公司 | Silymarin nanostructure lipid carrier and preparation method thereof |
| CN113616544A (en) * | 2021-08-05 | 2021-11-09 | 广东格烯生物科技股份有限公司 | Water-soluble ceramide NP composition and preparation process and application thereof |
| CN114177109A (en) * | 2021-12-17 | 2022-03-15 | 广州集妍化妆品科技有限公司 | Composite ceramide nano composition and preparation method and application thereof |
| CN114642610A (en) * | 2022-02-17 | 2022-06-21 | 广东丸美生物技术股份有限公司 | Moisturizing composition and preparation method and application thereof |
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Application publication date: 20191018 |