CN110305767A - Kit, nucleic acid extraction component and solution discard method - Google Patents
Kit, nucleic acid extraction component and solution discard method Download PDFInfo
- Publication number
- CN110305767A CN110305767A CN201910226780.4A CN201910226780A CN110305767A CN 110305767 A CN110305767 A CN 110305767A CN 201910226780 A CN201910226780 A CN 201910226780A CN 110305767 A CN110305767 A CN 110305767A
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- incorporating section
- waste liquid
- reagent
- kit
- water sucting
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 46
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 46
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000000605 extraction Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 181
- 239000002699 waste material Substances 0.000 claims abstract description 163
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 107
- 239000003153 chemical reaction reagent Substances 0.000 claims description 94
- 239000000463 material Substances 0.000 claims description 49
- 238000007789 sealing Methods 0.000 claims description 13
- 239000000284 extract Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 description 23
- 210000000078 claw Anatomy 0.000 description 6
- 239000000203 mixture Substances 0.000 description 4
- -1 polypropylene Polymers 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000010781 infectious medical waste Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000002734 metacrylic acid derivatives Chemical class 0.000 description 1
- 150000004702 methyl esters Chemical class 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920005629 polypropylene homopolymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229920005604 random copolymer Polymers 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to kit, nucleic acid extraction component and solution to discard method, kit has the main body with the waste liquid incorporating section configured with water sucting part, the waste liquid incorporating section has opening in the upper surface of the main body, is formed with notch in the upper surface for being set to bottom surface closer to the water sucting part of the position of the opening than the waste liquid incorporating section.
Description
Technical field
The present invention relates to the kit (reagent cartridge), nucleic acid extraction component and the solution that are accommodated with reagent are useless
Digging soil method.
Background technique
Due to the development of genetic test technology in recent years, enlighten tested such as by from the Biosample acquired from patient
Nucleic acid is extracted in object and gene difference as single nucleotide polymorphism is detected, it is possible to can be predicted in advance to medicine
The sensibility of product.
In the case where extracting nucleic acid from tested material, the diffusion that needs to prevent the cross contamination between sample, prevent the source of infection.
Therefore, reagent class needed for extracting nucleic acid from tested material is accommodated in kit, is used when extracting nucleic acid from tested material every time
Kit after using is discarded (referring for example to patent document 1) by reagent class stored to the greatest extent.
Nucleic acid extraction described in Patent Document 2 is formed with waste liquid hole with the kit contained in component in kit.Cause
This, can discard the waste liquid generated in nucleic acid extraction technique in waste liquid hole, and waste liquid is discarded together with kit.
It is preferred that the water sucting parts (referring for example to patent document 2) such as the sponge to absorb water to waste liquid are arranged in waste liquid hole.
Since the water sucting parts such as sponge absorb water to the waste liquid being present in inside waste liquid hole, even if the case where kit is toppled over
Under, can also it prevent waste liquid from flowing out.
Existing technical literature
Patent document
Patent document 1: International Publication No. 2005/118772
Patent document 2: No. 4911264 bulletins of Japanese Patent No.
Summary of the invention
Problems to be solved by the invention
It is whole in order to promptly implement nucleic acid extraction technique, it is also necessary to carry out waste liquid being discharged to waste liquid at high speed as far as possible
The processing in hole.But in the kit documented by patent document 2, waste liquid is being discharged at high speed from pipette to waste liquid hole
In the case of, the liquid measure per unit time being flowed into waste liquid hole be more than sometimes absorbed water by water sucting parts such as sponges it is every
The liquid measure of unit time and make waste liquid from waste liquid hole overflow.In addition, in the feelings that waste liquid is discharged at high speed from pipette to waste liquid hole
Under condition, waste liquid rebounds from water sucting parts such as sponges sometimes and disperses to the outside in waste liquid hole.
In view of the foregoing, waste liquid is discharged to waste liquid hole at high speed even if having the purpose of the present invention is to provide one kind
The kit in waste liquid hole that is interior, also can properly preventing waste liquid from flowing out to outside etc., the nucleic acid extraction group comprising the kit
Part and used the kit solution discard method.
Means for solving the problems
In order to solve the above problems, the invention proposes following scheme.
The kit of the 1st aspect of the present invention has the main body with the waste liquid incorporating section configured with water sucting part, described
Waste liquid incorporating section has opening in the upper surface of the main body, is being set to than the bottom surface of the waste liquid incorporating section closer to described
The upper surface of the water sucting part of the position of opening is formed with notch.
The notch can be formed in the central part of the upper surface of the water sucting part.
The notch depth of the notch can be from the end of the upper surface of the water sucting part close to the water suction
The part at the center of the upper surface of component is bigger.
The notch depth of the notch can be maximum at the center of the upper surface of the water sucting part.
The cut-out section of the notch can be V-shape.
It can be, the water sucting part is rectangular shape, the notch in such a way that triangle post shapes are cut off
It is formed.
The section vertical with the axis of the triangle post shapes can be regular triangle shape.
One side of the regular triangle shape can with by the length of the water sucting part on the short side direction of the main body
Spend the equal length of trisection.
The main body can also have tested material incorporating section and reagent incorporating section, and the tested material incorporating section is in the main body
The upper surface with tested material incorporating section be open, the reagent incorporating section the upper surface of the main body have reagent
Incorporating section opening, the tested material incorporating section that the opening of the waste liquid incorporating section is formed in the tested material incorporating section is opened
Between mouth and the reagent incorporating section opening of the reagent incorporating section.
Can also have to the sealing film for being open and being sealed.
The upper surface of the water sucting part can be set in the position lower than the opening of the waste liquid incorporating section.
The nucleic acid extraction component of the 2nd aspect of the present invention has kit and dispensing pipet stand, and the kit has
The reagent incorporating section of the reagent for extracting nucleic acid from tested material is stored, and configured with the water suction for being formed with notch in upper surface
Component and the waste liquid incorporating section that the unwanted solution separated in the process for extracting nucleic acid using the reagent is discarded, institute
It states dispensing pipet stand and is accommodated with the dispensing pipette for dispensing the reagent.
It is that will separate in the process for extracting nucleic acid using kit that the solution of the 3rd aspect of the present invention, which discards method,
The discarded solution of unwanted solution discards method, wherein the kit has the tested material incorporating section of storage tested material, receives
Receive the reagent incorporating section for having the reagent for extracting nucleic acid from the tested material, and configured with being formed with notch in upper surface
Water sucting part and the waste liquid storage that the unwanted solution separated in the process for extracting nucleic acid using the reagent is discarded
The dispensing pipette for dispensing the reagent is installed on the mouth of nucleic acid-extracting apparatus by portion, using being equipped with the dispensing liquid relief
The reagent for being accommodated in the reagent incorporating section is dispensed into the tested material incorporating section, uses the dispensing by the mouth of pipe
Pipette by the tested material incorporating section that dispensing has the reagent the unwanted solution that separates from the notch
Top is discharged to the waste liquid incorporating section.
Invention effect
Kit, nucleic acid extraction component and the solution of aforesaid way according to the present invention discard method, even if by waste liquid height
It is discharged in waste liquid hole fastly, also can properly prevent waste liquid to outside outflow etc..
Detailed description of the invention
Fig. 1 is the perspective view for indicating the overall structure of kit of first embodiment of the invention.
Fig. 2 is the dispensing pipet stand for indicating to be accommodated with multiple dispensing heads of pipette being used together with the kit
The perspective view of overall structure.
Fig. 3 is the cross-sectional view in the waste liquid hole in the kit.
Fig. 4 is the top view for being equipped with the nucleic acid-extracting apparatus of the kit.
Fig. 5 is the side view and top view for dispensing unit.
Fig. 6 is the cross-sectional view in the waste liquid hole inserted with dispensing head of pipette.
Fig. 7 is the cross-sectional view of the variation of the water sucting part of the kit.
Fig. 8 is the cross-sectional view of the variation of the water sucting part.
Fig. 9 is the cross-sectional view of the variation of the water sucting part.
Figure 10 is the cross-sectional view of the variation of the water sucting part.
Figure 11 is the cross-sectional view in the waste liquid hole in the kit of second embodiment of the present invention.
Symbol description
101 main bodys
The upper surface 101a
102 claws
103 handle portions
104 sealing films
110 sample wells (tested material incorporating section)
110a is open (tested material incorporating section opening)
120 reagent hole portions
121 reagent wells (reagent incorporating section)
121a is open (reagent incorporating section opening)
122 allotment holes
130 waste liquid holes (waste liquid incorporating section)
130a is open (waste liquid incorporating section opening)
131 water sucting parts
The upper surface 131a
131b notch
200 dispensing pipet stands
201 dispensing heads of pipette
The front end 201a
300 nucleic acid-extracting apparatus
304 dispensing units
Specific embodiment
(first embodiment)
First embodiment of the invention is illustrated referring to figs. 1 to Fig. 6.
Fig. 1 is the perspective view for indicating the overall structure of kit 100 of present embodiment.Fig. 2 be indicate to be accommodated with it is multiple
The perspective view of the overall structure of the dispensing pipet stand 200 for the dispensing head of pipette 201 being used together with kit 100.Fig. 4 is
The top view of the nucleic acid-extracting apparatus 300 of kit 100 is installed.
The reagent etc. for extracting nucleic acid from tested material is accommodated in kit 100.By the way that tested material is put into examination
It is installed on nucleic acid-extracting apparatus 300 in agent box 100, by kit 100, nucleic acid-extracting apparatus 300 can be made mechanically to implement to make
With the nucleic acid extraction operation of dispensing head of pipette 201.
<kit>
As shown in Figure 1, kit 100 has the main body 101 for being shaped generally as box-like, claw 102, handle portion 103 and close
Sealer 104.Fig. 1 shows the states that sealing film 104 (being indicated with double dot dash line) has been removed from main body 101.
As shown in Figure 1, claw 102 is formed as from the outer of an end (first end) on the length direction of main body 101
Surface is prominent, such as when kit 100 is installed on nucleic acid-extracting apparatus 300 and waits, claw 102 and the nucleic acid of kit 100 are mentioned
Take a part engaging of device 300.
As shown in Figure 1, handle portion 103 is formed in the outer of the other end (the second end) on the length direction of main body 101
Surface is engaged by claw 102 and handle portion 103 with a part of nucleic acid-extracting apparatus 300, can by kit 100
Nucleic acid-extracting apparatus 300 is fixed on by ground.It, can be by kit 100 from core in addition, by lifting handle portion 103 upwards
It is unloaded on sour extraction element 300 etc..
In the following description, the length direction of main body 101 is known as " X-axis ", the short side direction of main body 101 is known as " Y
The short transverse of the main body 101 orthogonal with X-axis and Y-axis is known as " Z axis " by axis ".In addition, in the X-axis direction, pawl will be formed with
One end (first end) in portion 102 is known as " front end ", is known as the other end (the second end) for being formed with handle portion 103
" cardinal extremity ".
Be integrally formed in main body 101 investment Biosample etc. the sample well (tested material incorporating section) 110 of tested materials,
Be accommodated with reagent etc. for extracting nucleic acid from tested material reagent hole portion 120 and will be in the process for extracting nucleic acid from tested material
The discarded waste liquid hole (waste liquid incorporating section) 130 of the unwanted solution of middle separation.
As shown in Figure 1, sample well 110, reagent hole portion 120 and waste liquid hole 130 are the only upper surface 101a in main body 101
Hole with opening.The upper surface 101a of main body 101, which successively has from the front end of X-direction towards cardinal extremity, is formed with reagent wells
The region of the opening in portion 120, be formed with waste liquid hole 130 opening 130a region and be formed with sample well 110 opening area
Domain.
Bottom surface sections (the upper surface in Z-direction of sample well 110, reagent hole portion 120 and each hole in waste liquid hole 130
Opposite side part) be enclosed.Reagent etc. can be put into from the opening for the upper surface 101a for being set to main body 101 to each hole
To be stored.
Reagent hole portion 120 is as shown in Figure 1, have multiple reagent wells (reagent incorporating section) 121 and allotment hole 122.
Reagent wells (reagent incorporating section) 121 are as shown in Figure 1, be to have round or rectangle in the upper surface 101a of main body 101
Opening (reagent incorporating section opening) 121a hole.It is accommodated with for example in reagent wells (reagent incorporating section) 121 for dissolving
The lysate of the biological substances such as cell membrane, the dissolution fluid for dissolving out nucleic acid from carrier, is used the dilution for dilute solution
In cleaning solution, the reagent for extracting nucleic acid from tested materials such as the solution containing magnetic particle of washing off unwanted solution.It
Be all reagent in present embodiment.It should be noted that the reagent stored in reagent wells 121 is not limited to these.
In addition, reagent can be accommodated in advance in reagent wells 121, reagent wells can also be accommodated in by user when in use
In 121.In particular it is preferred that being accommodated in when in use by the ethyl alcohol etc. that user would prove unsuitable for taking care of for a long time in kit 100
In reagent wells 121.
Deploying hole 122 is the hole for not being accommodated with reagent in advance, the reagent for storing in formulatory agents hole 121.From more
The reagent that a reagent wells 121 are taken out is put in allotment hole 122 and is deployed.
As shown in Figure 1, sample well (tested material incorporating section) 110 is in the upper surface 101a of main body 101 with circular
The columned hole of opening (tested material incorporating section opening) 110a.Sample well 110 is equipped with 2 in the base end side of main body 101, shape
As in the Z-axis direction than longer holes such as reagent wells 121 that reagent hole portion 120 is arranged in.In addition, 2 sample wells 110
It is spaced wider compared with the interval of multiple reagent wells 121 of reagent hole portion 120 is set, kit 100 is being installed on nucleic acid
When extraction element 300, heating portion, the magnet etc. that nucleic acid-extracting apparatus 300 has can be configured around sample well 110.
As shown in Figure 1, waste liquid hole (waste liquid incorporating section) 130 is that have short side and X-axis in the upper surface 101a of main body 101
In parallel and the concave hole of long side rectangular opening (waste liquid incorporating section opening) 130a parallel with Y-axis, hole are substantially
Rectangular shape.It will discard in the unwanted solution separated from the process that tested material extracts nucleic acid into waste liquid hole 130.
Configured with the water sucting part 131 to absorb water to waste liquid in waste liquid hole 130.Due to 131 pairs of water sucting part presence
Waste liquid inside waste liquid hole 130 absorbs water, therefore even if can also prevent waste liquid in the case where kit 100 is toppled over
Outflow.
Water sucting part 131 preferably such as with sponge for representative porous plastid, using Sodium Polyacrylate as the water suction of representative
Property macromolecule.
Fig. 3 is the cross-sectional view in the waste liquid hole 130 in the YZ plane vertical with X-axis.
Water sucting part 131 is the approximately cuboid shape by that will be accommodated in the waste liquid hole 130 of approximately cuboid shape
A part of the component of shape is cut away and is formed.Specifically, as shown in Figure 1, on water sucting part 131, in the opening side 130a
Upper surface 131a be formed with the " notch of the shape that with the axis that extends along the x axis and triangle post shapes are cut off
131b".Here, the upper surface 131a of water sucting part 131 is located at the position lower than opening 130a.
That is, upper surface 131a of the water sucting part 131 in the opening side 130a of waste liquid hole (waste liquid incorporating section) 130 is formed with and cuts
Oral area 131b.
In other words, it is being set to the suction of the bottom surface than waste liquid hole (waste liquid incorporating section) 130 closer to the position of opening 130a
The upper surface 131a of wet part 131 is formed with notch 131b.
As shown in figure 3, cut-out section of the notch 131b in YZ plane is V-shape.
It should be noted that the adjacent surface of the adjoining of the rectangular shape of water sucting part 131 not necessarily must be with right angle phase
It hands over, in addition, each face of rectangular shape also can have bumps.In these cases, notch 131b cutting in YZ plane
The V-shape in mouth section is not accurate V-shape sometimes.
As shown in figure 3, notch 131b is formed in the central part of the upper surface 131a of water sucting part 131 (comprising center
Region), in the center C of the upper surface 131a of water sucting part 131, notch depth D is (from upper surface 131a to the notch of Z-direction
Depth) reach maximum.Specifically, as shown in figure 3, the notch depth D of notch 131b is from the water sucting part 131
Upper surface 131a end (close to left end position, close to right end position) close to water sucting part 131 upper surface 131a
Center C part it is bigger.
In the present embodiment, the perpendicular to the axis of the triangle post shapes being cut off to form notch 131b cuts
Face is equilateral triangle.Therefore, as shown in figure 3, cut-out section of the notch 131b in YZ plane is the both sides by equilateral triangle
The V-shape of formation.In addition, the length on one side of equilateral triangle is as shown in figure 3, with by the length of the Y direction of water sucting part 131
Spend the equal length of trisection.
Between waste liquid hole 130 and water sucting part 131, there are the gap (richnesses in size in X-direction and Y direction
It is remaining), it can be easily by the configuration of water sucting part 131 in waste liquid hole 130 when manufacturing kit 100.
Water sucting part 131 can absorb the moisture of about 4000 μ l.For example it is about every time in the waste liquid for being discharged to waste liquid hole 130
In the case where 500 μ l, water sucting part 131 can absorb water about 8 times discharge amount waste liquid.
In main body 101, the opening of multiple reagent wells 121, allotment hole 122 and waste liquid hole 130 is sealed by sealing film 104.
Sealing film 104 preferably have be able to suppress gas transmission or by and can by be pierced into dispensing head of pipette 201 pierce
The composition of rupture of membranes, such as it is able to use the stacked film of the film of metallicity, plastic foil, their (films and plastic foil of metallicity)
Deng.
<dispensing pipet stand>
As shown in Fig. 2, dispensing pipet stand 200 has the dispensing head of pipette 201 of multiple same shapes, same size.
Since the liquid being accommodated in kit 100 carries out dispensing operation or stirring by any of multiple dispensing heads of pipette 201
Operation, so cross contamination will not be generated between the liquids using dispensing head of pipette 201.In addition, dispensing pipet stand 200
It is the container for recycling the dispensing head of pipette 201 after use, after dispense 201 use of head of pipette, can will divides
It is discarded together as infectious waste together with dispensing pipet stand 200 to infuse head of pipette 201.
As the material for forming main body 101, dispensing pipet stand 200 etc., as long as not had an impact to sample, reagent etc.
Material be then not particularly limited, especially with the resin material containing any one of polypropylene, polycarbonate, acrylic acid
When, it can be ensured that the state of solution can be confirmed in good visible light permeability.As polypropylene, can be used homo-polypropylene,
The random copolymer of polypropylene and polyethylene.In addition, polymethyl methacrylate or methyl-prop can be used as acrylic acid
The copolymer of the monomers such as e pioic acid methyl ester and other methacrylates, acrylate, styrene.In addition, using these resins
In the case where material, heat resistance, the intensity of dispensing head of pipette can also ensure that.
<nucleic acid-extracting apparatus>
Nucleic acid-extracting apparatus 300 is to be equipped with nucleic acid extraction component (kit 100 and dispensing pipet stand 200), automatic
Carry out the device of nucleic acid extraction.In addition, in the present embodiment, nucleic acid-extracting apparatus 300 automatically only carries out nucleic acid extraction, but
Nucleic acid-extracting apparatus 300 can also automatically be carried out from nucleic acid extraction up to real-time PCR, the detection of nucleic acid amplification reaction, nucleic acid
Analysis.
As shown in figure 4, having the kit setting unit 310 for kit 100 to be arranged, for dispensing pipet stand to be arranged
200 dispensing pipet stand setting unit 320, plate component 303 and dispensing unit 304.
Kit setting unit 310 be by be formed on plate component 303 opening, positioned at the vertical downside of plate component 303
The recess portion that support plate and the plate component vertical with Z-direction that the left of support plate, right and rear is arranged in are constituted.It is bowing
Apparent time, opening and support plate have the profile more slightly larger than the shape of kit setting unit 310, and the rear of opening is arranged in support plate
Side.For kit 100, the lower end of kit 100 is supported on vertical top by support plate, the side of kit 100 by
It is open and the plate component support at the left of support plate, right and rear is set, thus kit setting unit 310 is set.
Pipet stand setting unit 320 is dispensed by being formed in the opening of plate component 303 and positioned at the vertical downside of plate component 303
Support plate constitute.When looking down, opening has the profile more slightly larger than the shape for dispensing pipet stand setting unit 320.For dividing
Pipet stand 200 is infused, the lower end of dispensing pipet stand 200 is supported plate and is supported in vertical top, dispenses pipet stand 200
Side supported by plate component 303, be thus set to dispensing pipet stand setting unit 320.
Fig. 5 (a) (b) is the side view for dispensing unit 304, and Fig. 5 (c) is the top view for dispensing unit 304.
As shown in Fig. 5 (c), dispensing unit 304 has mouth 314, pump 315, upper and lower transferred unit (Z-direction transferred unit)
316, front and back transferred unit (X-direction transferred unit) 317 and left and right transferred unit (Y direction transferred unit) 318.
Mouth 314 can load and unload dispensing head of pipette 201 and aperture head of pipette 202.As shown in Fig. 5 (a), (b), if
The mouth 314 for dispensing unit 304 declines from the surface of dispensing head of pipette 201, then in the lower end of mouth 314, installation dispensing is moved
Liquid pipe head 201.Then, by rising mouth 314, dispensing head of pipette 201 is extracted from dispensing pipet stand 200.In mouth
Hole 314a is formed on 314, can attract and be discharged liquid from the lower end of mouth 314.
Pump 315 is connect with the hole 314a of mouth 314.It pumps 315 pairs of mouths 314 and assigns positive pressures and negative pressure, via being mounted on mouth
The dispensing head of pipette 201 of the lower end in portion 314 attracts and discharge liquid.
Upper and lower transferred unit 316 has the guide rail 316a and stepping motor being extended along Z axis.The upper and lower driving of transferred unit 316 step
Into motor, mouth 314 is transferred in the Z-axis direction along guide rail 316a.Front and back transferred unit 317 has the guide rail extended along an axis X
317a and stepping motor.Front and back transferred unit 317 drives stepping motor, transfers in the X-axis direction along guide rail 317a up and down
Transferred unit 316.Left and right transferred unit 318 has the guide rail 318a and stepping motor being extended along the Y axis.Left and right transfer member 318 drives
Dynamic stepping motor transfers front and back transfer member 317 along guide rail 318a in the Y-axis direction.
In this way, mouth 314 can be mentioned by upper and lower transferred unit 316, front and back transferred unit 317, left and right transferred unit 318 in nucleic acid
The inside of device 300 is taken to move along XYZ axis.
Unit 304 is dispensed by carrying out dispensing operation via the dispensing head of pipette 201 for being installed on mouth 314, in reagent
Tested material and reagent are transferred in box 100.
In the following, being illustrated to the effect of the kit 100 of composition described above.Hereinafter, to have carrying dispensing
The dispensing of head of pipette 201 is carried kit 100 is installed on the nucleic acid-extracting apparatus of function and extracts the operation of nucleic acid for, it is right
The effect of kit 100 is illustrated.
Firstly, the life for example as tested material is injected in the handwork by user into the sample well 110 of kit 100
Object sample.The kit 100 for having put into Biosample is installed on nucleic acid-extracting apparatus 300.
When attracting liquid into dispensing head of pipette 201 from reagent wells 121, the front end of head of pipette 201 will be dispensed
201a is inserted into the sealing film 104 in sealed reagent hole 121.Then, through hole is formed on sealing film 104, by dispensing liquid relief
Tube head 201 can attract the various reagents class inside reagent wells 121.
Then, dispensing head of pipette is used according to the sequence of regulation using the dispensing unit 304 of nucleic acid-extracting apparatus 300
The various reagents that 201 dispensings, mixing are stored in reagent wells 121.It is supplied to as a result, thin in the Biosample of sample well 110
Born of the same parents are dissolved, obtain cytolysate.
Attracted in the unwanted solution separated from the process that tested material extracts nucleic acid by dispensing head of pipette 201, gives up
It abandons in waste liquid hole 130.The front end 201a of insertion dispensing head of pipette 201 in the sealing film 104 in sealing waste liquid hole 130.In
It is to form through hole H1 on sealing film 104, can be discarded waste liquid in waste liquid hole 130 by dispensing head of pipette 201
Portion.
The waste liquid hole 130 in YZ plane when Fig. 6 is the insertion waste liquid hole 130 front end 201a for dispensing head of pipette 201
Cross-sectional view.
As shown in fig. 6, the viewpoint grade of the intensity after the viewpoint of through hole H1 easy to form, formation through hole H1,
Through hole H1 is formed in the center of the opening 130a in waste liquid hole 130.
The front end 201a for dispensing head of pipette 201 is penetrated through from through hole H1, therefore as shown in fig. 6,201a configuration in front end exists
The position opposite with the upper surface 131a of notch 131b is formed with.In addition, since through hole H1 is formed in opening 130a
The heart, so the front end 201a of dispensing head of pipette 201 is as shown in fig. 6, configuration is maximum with notch depth D in the 131a of upper surface
The opposite position in center.
When waste liquid is discharged from dispensing head of pipette 201 to waste liquid hole 130, in the front end from dispensing head of pipette 201
The direction i.e. lower section (the lower direction of Z-direction) of 201a discharge waste liquid is formed with notch 131b, therefore as shown in Figure 6, it is ensured that
Region (temporarily taking care of region) T of the waste liquid before absorbing water can be temporarily taken care of there is no water sucting part 131.Therefore, even if
In the case where waste liquid to be discharged to waste liquid hole at high speed, the waste liquid before also water sucting part 131 can absorb water temporarily is taken care of
In interim keeping region T.Even if being more than to be absorbed water by water sucting part 131 in the liquid measure per unit time for flowing into waste liquid hole 130
In the case where liquid measure per unit time, it also can properly prevent waste liquid from overflowing from waste liquid hole 130.
Cut-out section of the notch 131b in YZ plane is illustrated in figure 6 V-shape, therefore from dispensing head of pipette
The section for the notch 131b that the waste liquid of 201 front end 201a discharge can rebound is not parallel with upper surface 131a.Therefore, it is cutting
The waste liquid to rebound on the section of oral area 131b not rebound and to different direction bullet upwards as the crow flies by (top of Z-direction)
It returns.It disperses as a result, it is possible to properly prevent the waste liquid to rebound from flowing out from through hole H1 to the outside in waste liquid hole 130.
As shown in fig. 6, dispensing head of pipette 201 front end 201a configured in the 131a of upper surface with notch depth D most
The opposite position in big center.From the waste liquid that the front end 201a of dispensing head of pipette 201 is discharged to the section with notch 131b
The waste liquid of the distance of contact, the section for the notch 131b that can properly prevent from rebounding is flowed out from through hole H1 and is dispersed to useless
The outside of fluid apertures 130.
Cut-out section of the notch 131b in YZ plane is illustrated in figure 6 V-shape, therefore, in order to form notch
Portion 131b and by the fixing fabric structure for the part being cut off on the basis of as small as possible, be capable of increasing the surface of water sucting part 131
Product.
The volume for the part cut away and reducing as far as possible in order to form notch 131b, is able to suppress water sucting part
131 volume is reduced, and inhibits the reduction of the water suction liquid measure of water sucting part 131.On the other hand, by increasing water sucting part 131
Surface area is capable of increasing the liquid measure per unit time that water sucting part 131 is absorbed.
As shown in Figure 1, the opening that opening (the waste liquid incorporating section opening) 130a in waste liquid hole is formed in sample well 110 is (tested
Object incorporating section opening) between 110a and opening (reagent incorporating section opening) 121a of reagent wells 121.Therefore, either in sample
The waste liquid generated in hole 110, or the waste liquid generated in reagent wells 121, can be suitable by the waste liquid with shorter portable cord
It is discharged to waste liquid hole 130 sharply.In addition, needing to make to dispense in the case where dispensing reagent from reagent wells 121 to sample well 110
Head of pipette 201 is mobile across 130 ground of waste liquid hole, even if waste liquid hole 130 is former in the case where reagent falls on waste liquid hole 130
This is also the place of discharge waste liquid, therefore will not become problem.
Kit 100 according to the present embodiment also can be proper even if waste liquid is discharged at high speed in waste liquid hole 130
Locality prevents waste liquid to outside outflow etc..
In addition, the solution of present embodiment discards method for example it is also possible to that bright kit 100 will be stated in use
It extracts the discarded solution of the unwanted solution separated in the process of nucleic acid and discards method, wherein the kit 100 has receipts
It receives the tested material incorporating section 110 of tested material, is accommodated with the reagent incorporating section of the reagent for extracting nucleic acid from the tested material
121, and will extract configured with the water sucting part 131 for being formed with notch 131b in upper surface 131a and using the reagent
The discarded waste liquid incorporating section 130 of the unwanted solution separated in the process of nucleic acid, will dispense the dispensing pipette of the reagent
It is installed on the mouth of nucleic acid-extracting apparatus, using the mouth 314 for being mounted with the dispensing pipette, the reagent will be accommodated in and received
The reagent received in portion 121 is dispensed into the tested material incorporating section 110, using the dispensing pipette will dispensing
It states the unwanted solution separated in the tested material incorporating section 110 of reagent and is discharged to institute above the notch 131b
State waste liquid incorporating section 130.
More than, first embodiment of the invention is described in detail referring to attached drawing, but specific constitutes and unlimited
It further include the design alteration etc. for the range for not departing from present subject matter in the embodiment.In addition, in above-mentioned first embodiment
And constituent element shown in variation as shown below can also be constituted with appropriately combined.
(variation 1)
For example, in the above-described embodiment, kit 100 has sample well 110, reagent hole portion 120 and waste liquid hole
130, but it's not limited to that for the composition of kit.Kit for example can only have waste liquid hole 130, can also be also equipped with other
The hole (incorporating section) of purposes.
(variation 2)
For example, in the above-described embodiment, it is the both sides shape by equilateral triangle that cut-out section is formed on water sucting part 131
At V-shape notch 131b, but it's not limited to that for the mode of notch.The shape of notch is also possible to notch and cuts
The V-shape of the different mode in face.Alternatively, it is also possible to water sucting part as shown in Figure 7 variation, that is, water sucting part 131B that
Sample, the cut-out section of notch are U-shaped.As shown in fig. 7, notch 131Bb is formed in the upper surface of water sucting part 131B
The central part (region comprising center) of 131Ba.Water sucting part 131B and water sucting part 131 are same, even if at high speed by waste liquid
It is discharged in waste liquid hole 130, also can properly prevent waste liquid to outside outflow etc..
(variation 3)
For example, in the above-described embodiment, notch made of cutting away triangle post shapes is formed on water sucting part 131
131b, but it's not limited to that for the mode of notch.The shape of notch can also be as shown in Figure 8 water sucting part variation
That is water sucting part 131C is such, and being formed to have to cut away has made of the cone shape for the axis that Z-direction extends shape
Notch 131Cb.As shown in figure 8, the notch 131Cb of water sucting part 131C is formed in the upper surface of water sucting part 131C
The central part (region comprising center) of 131Ca.In addition, the notch depth D of notch 131Cb is (from upper surface 131Ca to Z axis
The depth of the notch in direction) it is maximum at the center of the upper surface 131Ca of water sucting part 131C.Water sucting part 131C and water-holding portion
Part 131 is same, even if waste liquid is discharged at high speed in waste liquid hole 130, also can properly prevent waste liquid to outside outflow etc..
(variation 4)
For example, in the above-described embodiment, it is the both sides shape by equilateral triangle that cut-out section is formed on water sucting part 131
At V-shape notch 131b, but it's not limited to that for the mode of notch.The shape of notch can also be such as Fig. 9 institute
The i.e. water sucting part 131D of the variation of the water sucting part shown is such, and cut-out section is concave shape.Water sucting part 131D can not make
The waste liquid for springing back to the section of notch 131Db rebounds to the direction different from top (top in Z-direction), but by facing
When take care of region T, can properly prevent waste liquid from waste liquid hole 130 overflow.In addition, passing through the surface for increasing water sucting part 131D
Product, is capable of increasing the liquid measure per unit time that water sucting part 131D is absorbed.
(variation 5)
For example, in the above-described embodiment, waste liquid hole 130 is approximately cuboid shape, and water sucting part 131 is by will
The a part for being enough accommodated in the component of the approximately cuboid shape in the waste liquid hole 130 of approximately cuboid shape is cut away and is formed.
But it's not limited to that for the shape of waste liquid hole and water sucting part.Waste liquid hole can also be as shown in Figure 10 kit deformation
Example is that the waste liquid hole 130B of kit 100B is such, is to have circular opening 130Ba in the upper surface 101a of main body 101B
Columned hole.In this case, as shown in Figure 10, water sucting part 131E is by that will be accommodated in the useless of cylindrical shape
A part of the component of the cylindrical shape of fluid apertures 130B is cut away and is formed.As shown in Figure 10, in the upper surface 131Ea of open side
Central part (region comprising center) be formed with will have along the cone shape for the axis that Z-direction extends cut away made of cut
The notch depth D of oral area 131Eb, notch 131Eb are leaned on (from the peripheral part of the upper surface 131Ea of water sucting part 131E)
The part at the center of the upper surface 131Ea of nearly water sucting part 131E is bigger.The cut-out section of the Z-direction of notch 131Eb is V
Word shape.Water sucting part 131E and water sucting part 131 are same, even if waste liquid is discharged at high speed in the 130B of waste liquid hole, also can
It is enough properly to prevent waste liquid to outside outflow etc..
Notch is formed alternatively, it is also possible to have the triangle post shapes of the axis extended along Z-direction to cut away.
(second embodiment)
1 pair of second embodiment of the present invention is illustrated referring to Fig.1.In the following description, to have been described above
Constitute that identical composition marks identical appended drawing reference and the repetitive description thereof will be omitted.
The kit 100C of second embodiment compared with the kit 100 of first embodiment, the difference is that, hold
The shape of the water sucting part 131F in waste liquid hole 130 is contained in relative to Z axis asymmetry.
Figure 11 is the cross-sectional view in the waste liquid hole 130 of kit 100C, and the front end 201a of dispensing head of pipette 201 is inserted into examination
In the waste liquid hole 130 of agent box 100C.
Kit 100C and the kit of first embodiment 100 are same, have be shaped generally as box-like main body 101,
Claw 102, handle portion 103 and sealing film 104.The water sucting part 131F being only contained in the waste liquid hole 130 of main body 101 and
The kit 100 of one embodiment is different.
Water sucting part 131F is the approximately cuboid shape by that will be accommodated in the waste liquid hole 130 of approximately cuboid shape
A part of the component of shape is cut away and is formed.Specifically, as shown in figure 11, on water sucting part 131F, in opening 130a
The upper surface 131Fa of side is formed with " notch made of will there are the triangle post shapes of axis extended along the x axis to cut away
131Fb”。
As shown in figure 11, cut-out section of the notch 131Fb in YZ plane is V-shape.Here, V-shape is cut
Mouth section is relative to Z axis asymmetry.
As shown in figure 11, notch 131Fb is formed in the central part of the upper surface 131Fa of water sucting part 131F (in including
The region of the heart).As shown in figure 11, notch depth D of the notch 131Fb at the center of the upper surface 131Fa of water sucting part 131F
Not maximum, notch depth D reaches maximum at the part at center for not being upper surface 131Fa.
Since cut-out section of the notch 131Fb in YZ plane is V-shape, from dispensing head of pipette 201
The section for the notch 131Fb that the waste liquid of front end 201a discharge can rebound and upper surface 131Fa are not parallel.Therefore, it springs back to
The waste liquid in the section of notch 131Fb not rebound upwards as the crow flies by (top in Z-direction), but to different direction bullets
It returns.
It as shown in figure 11, is being lower section (Z-direction from the direction of the front end 201a discharge waste liquid of dispensing head of pipette 201
On lower section), the section for the notch 131Fb that waste liquid can rebound obliquely is provided with relative to Z axis.Therefore, in notch
The waste liquid that the section of 131Fb is rebounded is as shown in figure 11, energetically rebounds to the right of Y-axis.As a result, it is possible to properly prevent bullet
The waste liquid returned is flowed out from through hole H1 and is dispersed to the outside in waste liquid hole 130.
Kit 100C according to the present embodiment also can be proper even if waste liquid is discharged at high speed in waste liquid hole 130
Locality prevents waste liquid to outside outflow etc..
In the case where making waste liquid be expelled to waste liquid hole at high speed, even if in the liquid per unit time flowed into waste liquid hole
It, also can be proper by temporarily taking care of region T in the case that amount is more than the liquid measure per unit time to be absorbed water by water sucting part 131F
Locality prevents waste liquid from overflowing from waste liquid hole 130.In addition, waste liquid rebounds from water sucting part 131F by notch 131Fb, it can
Properly prevent the waste liquid to rebound from flowing out and being dispersed to the outside in waste liquid hole 130 from through hole H1.
In addition, in the above-described embodiment, the cut-out section in YZ plane is asymmetrical, but the mode of cut-out section is simultaneously
It is not limited to this.XZ plane etc. is also possible to asymmetrical comprising the cut-out section in the plane of Z axis.In this case, also can
Enough control to make direction of the direction not towards the position configured with through hole H1 of the waste liquid to rebound from cut-out section.
The present invention can be applied to the kit for having waste liquid hole.
The preferred embodiments of the present invention is illustrated above, but these are illustrative explanations of the invention,
It should be understood that its content that should not be regarded as limiting.It is able to carry out additional, province without departing from the present invention
Slightly, replacement and other changes.Therefore, the present invention should not be regarded as being defined in above-mentioned explanation, but by claims into
Row limits.
Claims (17)
1. a kind of kit has the main body with the waste liquid incorporating section configured with water sucting part,
The waste liquid incorporating section has opening in the upper surface of the main body,
Be set to the bottom surface than the waste liquid incorporating section closer to the position of the opening the water sucting part upper surface
It is formed with notch.
2. kit according to claim 1, wherein
The notch is formed in the central part of the upper surface of the water sucting part.
3. kit according to claim 1, wherein
The notch depth of the notch is from the end of the upper surface of the water sucting part close to the institute of the water sucting part
The part for stating the center of upper surface is bigger.
4. kit according to claim 1, wherein
The notch depth of the notch is maximum at the center of the upper surface of the water sucting part.
5. kit according to claim 1, wherein
The cut-out section of the notch is V-shape.
6. kit according to claim 5, wherein
The water sucting part is rectangular shape,
The notch is formed in such a way that triangle post shapes are cut off.
7. kit according to claim 6, wherein
The section vertical with the axis of the triangle post shapes is regular triangle shape.
8. kit according to claim 7, wherein
One side of the regular triangle shape with by the length trisection of the water sucting part on the short side direction of the main body
Equal length.
9. kit described according to claim 1~any one of 8, wherein
The main body also has tested material incorporating section and reagent incorporating section,
The tested material incorporating section is open in the upper surface of the main body with tested material incorporating section,
The reagent incorporating section is open in the upper surface of the main body with reagent incorporating section,
The opening of the waste liquid incorporating section is formed in tested material incorporating section opening and the institute of the tested material incorporating section
It states between the reagent incorporating section opening of reagent incorporating section.
10. kit described according to claim 1~any one of 8 is also equipped with to the sealing for being open and being sealed
Film.
11. kit according to claim 9 is also equipped with to the sealing film for being open and being sealed.
12. kit described according to claim 1~any one of 8, wherein
The position lower than the opening of the waste liquid incorporating section is arranged in the upper surface of the water sucting part.
13. kit according to claim 9, wherein
The position lower than the opening of the waste liquid incorporating section is arranged in the upper surface of the water sucting part.
14. kit according to claim 10, wherein
The position lower than the opening of the waste liquid incorporating section is arranged in the upper surface of the water sucting part.
15. kit according to claim 11, wherein
The position lower than the opening of the waste liquid incorporating section is arranged in the upper surface of the water sucting part.
16. a kind of nucleic acid extraction component has kit and dispensing pipet stand,
The kit has the reagent incorporating section of reagent of the storage for extracting nucleic acid from tested material, and configured in upper surface
It is formed with the water sucting part of notch and the unwanted solution separated in the process for extracting nucleic acid using the reagent gives up
The waste liquid incorporating section of abandoning,
The dispensing pipet stand is accommodated with the dispensing pipette for dispensing the reagent.
It is the unwanted solution that will be separated in the process for extracting nucleic acid using kit 17. a kind of solution discards method
Discarded solution discards method, wherein
The kit has the tested material incorporating section of storage tested material, is accommodated with the examination for extracting nucleic acid from the tested material
The reagent incorporating section of agent, and will extract configured with the water sucting part for being formed with notch in upper surface and using the reagent
The discarded waste liquid incorporating section of the unwanted solution separated in the process of nucleic acid,
The dispensing pipette for dispensing the reagent is installed on to the mouth of nucleic acid-extracting apparatus,
Using the mouth for being equipped with the dispensing pipette, the reagent for being accommodated in the reagent incorporating section is dispensed into described
Tested material incorporating section,
It will be separated in the tested material incorporating section that dispensing has the reagent using the dispensing pipette unwanted molten
Liquid is discharged to the waste liquid incorporating section above the notch.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2018060489A JP2019174188A (en) | 2018-03-27 | 2018-03-27 | Reagent cartridge, nucleic acid extraction set, and method of disposing of solution |
| JP2018-060489 | 2018-03-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110305767A true CN110305767A (en) | 2019-10-08 |
Family
ID=68074535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910226780.4A Pending CN110305767A (en) | 2018-03-27 | 2019-03-25 | Kit, nucleic acid extraction component and solution discard method |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JP2019174188A (en) |
| CN (1) | CN110305767A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111876312A (en) * | 2020-08-12 | 2020-11-03 | 济南国益生物科技有限公司 | Nucleic acid extraction kit and nucleic acid extraction method |
| CN113462556A (en) * | 2021-09-02 | 2021-10-01 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
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| Publication number | Publication date |
|---|---|
| JP2019174188A (en) | 2019-10-10 |
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