CN110229865A - A kind of pollen pini active peptide extracting method - Google Patents
A kind of pollen pini active peptide extracting method Download PDFInfo
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- CN110229865A CN110229865A CN201910622191.8A CN201910622191A CN110229865A CN 110229865 A CN110229865 A CN 110229865A CN 201910622191 A CN201910622191 A CN 201910622191A CN 110229865 A CN110229865 A CN 110229865A
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- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The invention discloses a kind of pollen pini active peptide extracting methods, belong to active peptide technical field.The purpose of the present invention is to provide one kind to have the pharmacological activity such as anti arteriosclerosis, reducing blood lipid and cholesterol, analgesia, anti-radiation, prevention and treatment diabetes, strengthen immunity, anti-aging, cancer-resisting, and absorption easy to digest, body utilization rate full of nutrition is high, strong operability, it is easy to industrialized production, the high pollen pini active peptide extracting method of recovery rate.
Description
Technical field
The invention belongs to active peptide technical fields, and in particular to a kind of pollen pini active peptide and its extracting method.
Background technique
Pollen pini (English: Pine pollen) also known as preserved egg, pine are yellow, refer to masson pine, Chinese pine, Korean pine, Huashan pine and camphor tree
Dry pollen caused by the pine genus plants stamen such as sub- pine.It is foresythia or faint yellow thin first recorded in Eastern Han Dynasty's Shennong's Herbal
Powder, sweet taste is nontoxic, is the food of Chinese tradition, and some Chinese tradition foods such as preserved egg cake, preserved egg dumpling, preserved egg wine etc. still add
Add pollen pini.There is very big medical value, it is significant in efficacy for skin related disease, it is received by medical ancient books and records many since ancient times
Record, including " people's republic, China, China pharmacopeia ".Pollen pini is the spermatid of pine tree pistil, is responsible for the important task of pine tree procreation,
More than 200 nutritions are included, and are arranged in pairs or groups rationally, can be supplemented comprehensively, nutrition needed for balancing human body.Its health-care effect is big,
It is wide to improve range, is qualified " healthy bodyguard ".Thus, the user of all ages and classes, different constitutions can be shorter
In time, good effect is received to varying degrees.
Pollen pini active peptide is to close object original through specific pollen pini active peptide manufactured by enzyme hydrolysis with broken wall preserved egg pollen
Material, no protein contain 22 kinds of amino acid, 14 kinds of vitamins and 30 various trace elements and a large amount of active protease, core
Acid, flavone compound and other active materials.
Modern nutriology research discovery: the mankind ingest protein after gastral enzyme effect, are in the form of low peptide mostly
It digests and assimilates, the ratio very little absorbed in the form of free amino acid；Human body can independently inhibit to rouge when being rich in active peptide
The absorption of fat, active peptide are had an effect with fat, generate lipase, can promote the decomposition of fat.Further test discloses again
Peptide specific ionization amino acid digestion faster, absorb more, show that the biological value of peptide and nutritive value specific ionization amino acid are higher.
This is also exactly the infinite glamour place of active peptide.
Biologically active peptide be in protein 20 natural amino acids with it is different form and arrangement mode constituted slave dipeptides to
The general name of complicated linear, ring structure different peptides, is derived from the multi-functional compounds of protein.Active peptide has a variety of
Body metabolism and physiological regulation function, absorption easy to digest have immune promotion, hormone control, antibacterial, antiviral, blood pressure lowering, drop blood
The effects of rouge, edible safety is high, is the function of current international food circle most popular research topic and great development prospect
The factor.
The lesser small peptide of some molecular weight in biologically active peptide, compared with amino acid transport function, in biologically active peptide
Small peptide have the characteristics that absorb quickly, low consumption and unsaturated.Scientific circles and medical field can be some special statuss using its feature
Under crowd supplement the nutrients, such as postoperative wound healing stage, convalescence, physical rehabilitation phase；Stress is big, overworked, appetite
It is depressed；Chemical substance, heavy metal, radiation storage, remains of pesticide in food, it is long to take chemicals, Chinese medicine residual poison, digestive system damage
Functions of intestines and stomach imbalance person caused by evil；Amount of exercise is big, physical labor intensity is big must be replenished in time nitrogen source, and not can increase stomach function
It can bearer；Digestive system cannot be into trencherman because of illness obstacle；The infant of digestive organs undeveloped mature, digestion and absorption function
The old man to fall into a decline.Small peptide in biologically active peptide is to multiple beneficials such as lactic acid bacteria, Bifidobacterium Bifidum and saccharomycete in human body
The growth of vitamins remarkably promotes effect, this is of great significance to the digestion and absorption of food nutrition.
Therefore, the problem of a kind of method that active peptide is efficiently extracted from pollen pini becomes urgent need to resolve, application are developed
Number a kind of extracting method of pollen pini active peptide is disclosed for 201710611722.4 Chinese patents, using multienzyme to pollen pini
Digested, but its there are complex process, the not high problems of pollen pini activity peptides extraction rate.
Summary of the invention
The purpose of the present invention is to provide one kind to have anti arteriosclerosis, reducing blood lipid and cholesterol, analgesia, anti-radiation, anti-
Control the pharmacological activity such as diabetes, strengthen immunity, anti-aging, cancer-resisting, and absorption easy to digest, body benefit full of nutrition
With rate height, strong operability is easy to industrialized production, the high pollen pini active peptide extracting method of recovery rate.
The object of the present invention is achieved like this, and the pollen pini after broken wall passes through cellulase degradation stomach function regulating protease hydrolyzed
Later, pepsin enzymolysis liquid is dried to obtain pollen pini active peptide.
Preferably, the cellulase degradation step are as follows: after the pollen pini of broken wall and purified water are stirred evenly,
It is separately added into hydrochloric acid and cellulase, is heated to steady temperature, keeps temperature constant, after a period of time, cellulase is made
Enzymolysis liquid.
Preferably, the pepsin enzymolysis step are as follows: take cellulase degradation liquid, sequentially add pepsin and
Hydrochloric acid, heat preservation after stirring certain time, continue to be heated to 100 DEG C, after keeping the temperature a period of time, be cooled to room temperature, adjust pH
Value is 6.5-7.5, and centrifugation collects supernatant liquor and obtains pepsin enzymolysis liquid.
Preferably, the drying mode of pepsin enzymolysis liquid is spray drying, after dry, collects the powder in collection material frame
Agent is up to pollen pini active peptide.
Pollen pini active peptide is obtained using pollen pini active peptide extracting method described above.
Beneficial effects of the present invention:
1, using pollen pini active peptide extracting method of the present invention, pollen pini activity peptides extraction rate is high, i.e., for this
For invention, acid enzymatic hydrolysis is carried out to the pollen pini after broken wall, acidity enzymatic hydrolysis is divided into cellulase degradation and pepsin enzyme
Solution, either cellulase or pepsin, the environment of best enzymolysis efficiency are acidity, make full use of cellulase and
Stomach cardia enzyme viability extracts the active peptide in pollen pini.
2, pollen pini active peptide extracting method of the present invention, simple process is easy to promote large-scale, joins to technique
Number control requires lower, to equipment high recycling rate, reduces the scale of investment of enterprise.
It 3, can be directly as edible using the extracted active peptide of pollen pini active peptide extracting method of the present invention
The pollen pini active peptide of grade, the i.e. present invention do not add any additive or organic solvent extract, do not need to add organic solvent
The recovery process of extractant, and its extraction process is safe and efficient.
4, the extraction process of pollen pini active peptide is divided into multiple steps by the present invention, is conducive to enterprise work personnel or technology people
The grasp of member, and can timely track production problem.
5, active peptide is extracted using pollen pini active peptide extracting method of the present invention, pollen pini active peptide will not be destroyed
In function factor, ensure that quality of finished.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated, but the present invention is limited in any way,
Based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.
Embodiment 1
Pollen pini after broken wall passes through after cellulase degradation stomach function regulating protease hydrolyzed, and pepsin enzymolysis liquid is dry
Obtain pollen pini active peptide.
The cellulase degradation step are as follows: after stirring evenly the pollen pini of broken wall and purified water, be separately added into
Hydrochloric acid and cellulase are heated to steady temperature, keep temperature constant, and after a period of time, cellulase degradation liquid is made.
The pepsin enzymolysis step are as follows: take cellulase degradation liquid, sequentially add pepsin and hydrochloric acid, protect
After temperature, stirring certain time, continue to be heated to 100 DEG C, after keeping the temperature a period of time, be cooled to room temperature, adjusting pH value is
6.5-7.5, centrifugation collect supernatant liquor and obtain pepsin enzymolysis liquid.
The drying mode of pepsin enzymolysis liquid is spray drying, after dry, collects the pulvis in collection material frame up to pine
Pollen active peptide.
Embodiment 2
Pollen pini after broken wall passes through after cellulase degradation stomach function regulating protease hydrolyzed, and pepsin enzymolysis liquid is dry
Obtain pollen pini active peptide.
The cellulase degradation step are as follows: take broken masson pine pollen 1Kg, add purified water 4Kg, stir evenly, 6mol/L is added
Hydrochloric acid 2-4ml adjusts pH value most 4.5-6.5, and cellulase 9-11g is added, and is heated to 26 DEG C, keeps the temperature 3 hours to get cellulose
Enzyme enzymolysis liquid.
The pepsin enzymolysis step are as follows: it takes above-mentioned cellulase degradation liquid, is added pepsin 8-10g, then plus
Enter 6mol/L hydrochloric acid 5-7ml, keeps the temperature 35-50 DEG C and hydrolyze 2 hours, wherein persistently stirred in hydrolytic process, continue to be heated to 100
DEG C, 10 minutes are kept the temperature, room temperature to be cooled, adjusting pH value is 6.5, is centrifuged with 3800r/min, collects supernatant, obtains gastric enzyme enzymolysis liquid.
Take above-mentioned gastric enzyme enzymolysis liquid, move to spray dryer: 165 DEG C of air intake vent, air outlet: 85 DEG C, spray drying 3 is small
When, the pulvis in material frame is collected, pollen pini active peptide powder is obtained.
Embodiment 3
Pollen pini after broken wall passes through after cellulase degradation stomach function regulating protease hydrolyzed, and pepsin enzymolysis liquid is dry
Obtain pollen pini active peptide.
The cellulase degradation step are as follows: take broken masson pine pollen 1Kg, add purified water 4Kg, stir evenly, 6mol/L is added
Hydrochloric acid 2ml adjusts pH value most 6.5, and cellulase 9g is added, and is heated to 26 DEG C, keeps the temperature 3 hours to get cellulase degradation liquid.
The pepsin enzymolysis step are as follows: take above-mentioned cellulase degradation liquid, pepsin 8g is added, adds
6mol/L hydrochloric acid 5ml keeps the temperature 35 DEG C and hydrolyzes 2 hours, wherein persistently stirs in hydrolytic process, continues to be heated to 100 DEG C, heat preservation 10
Minute, room temperature to be cooled, adjusting pH value is 6.5, is centrifuged with 3800r/min, collects supernatant, obtains gastric enzyme enzymolysis liquid.
Take above-mentioned gastric enzyme enzymolysis liquid, move to spray dryer: 165 DEG C of air intake vent, air outlet: 85 DEG C, spray drying 3 is small
When, the pulvis in material frame is collected, pollen pini active peptide powder is obtained.
Embodiment 4
Pollen pini after broken wall passes through after cellulase degradation stomach function regulating protease hydrolyzed, and pepsin enzymolysis liquid is dry
Obtain pollen pini active peptide.
The cellulase degradation step are as follows: take broken masson pine pollen 1Kg, add purified water 4Kg, stir evenly, 6mol/L is added
Hydrochloric acid 4ml adjusts pH value most 4.5, and cellulase 11 g is added, and is heated to 26 DEG C, keeps the temperature 3 hours to get cellulase degradation
Liquid.
The pepsin enzymolysis step are as follows: take above-mentioned cellulase degradation liquid, pepsin 10g is added, adds
6mol/L hydrochloric acid 7ml keeps the temperature 50 DEG C and hydrolyzes 2 hours, wherein persistently stirs in hydrolytic process, continues to be heated to 100 DEG C, heat preservation 10
Minute, room temperature to be cooled, adjusting pH value is 6.5, is centrifuged with 3800r/min, collects supernatant, obtains gastric enzyme enzymolysis liquid.
Take above-mentioned gastric enzyme enzymolysis liquid, move to spray dryer: 165 DEG C of air intake vent, air outlet: 85 DEG C, spray drying 3 is small
When, the pulvis in material frame is collected, pollen pini active peptide powder is obtained.
Embodiment 5
Pollen pini after broken wall passes through after cellulase degradation stomach function regulating protease hydrolyzed, and pepsin enzymolysis liquid is dry
Obtain pollen pini active peptide.
The cellulase degradation step are as follows: take broken masson pine pollen 1Kg, add purified water 4Kg, stir evenly, 6mol/L is added
Hydrochloric acid 3ml, adjusting pH value is 5, and cellulase 10g is added, and is heated to 26 DEG C, keeps the temperature 3 hours to get cellulase degradation liquid.
The pepsin enzymolysis step are as follows: take above-mentioned cellulase degradation liquid, pepsin 9g is added, adds
6mol/L hydrochloric acid 6ml keeps the temperature 45 DEG C and hydrolyzes 2 hours, wherein persistently stirs in hydrolytic process, continues to be heated to 100 DEG C, heat preservation 10
Minute, room temperature to be cooled, adjusting pH value is 6.5, is centrifuged with 3800r/min, collects supernatant, obtains gastric enzyme enzymolysis liquid.
Take above-mentioned gastric enzyme enzymolysis liquid, move to spray dryer: 165 DEG C of air intake vent, air outlet: 85 DEG C, spray drying 3 is small
When, the pulvis in material frame is collected, pollen pini active peptide powder is obtained.
Claims (5)
1. a kind of pollen pini active peptide extracting method, which is characterized in that the pollen pini after broken wall passes through cellulase degradation stomach function regulating
After protease hydrolyzed, pepsin enzymolysis liquid is dried to obtain pollen pini active peptide.
2. a kind of pollen pini active peptide extracting method according to claim 1, which is characterized in that the cellulose enzyme
Solve step are as follows: after stirring evenly the pollen pini of broken wall and purified water, be separately added into hydrochloric acid and cellulase, be heated to constant
Temperature keeps temperature constant, and after a period of time, cellulase degradation liquid is made.
3. a kind of pollen pini active peptide extracting method according to claim 2, which is characterized in that the pepsin enzyme
Solve step are as follows: take cellulase degradation liquid, sequentially add pepsin and hydrochloric acid, heat preservation after stirring certain time, continues to add
Heat after keeping the temperature a period of time, is cooled to room temperature to 100 DEG C, and adjusting pH value is 6.5-7.5, and centrifugation collects supernatant liquor and obtains stomach
Protease hydrolyzed liquid.
4. a kind of pollen pini active peptide extracting method according to claim 3, which is characterized in that pepsin enzymolysis liquid
Drying mode is spray drying, after dry, collects the pulvis in collection material frame up to pollen pini active peptide.
5. a kind of pollen pini active peptide, which is characterized in that using the activity of pollen pini described in any one of Claims 1-4
Peptide extracting method and obtain.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910622191.8A CN110229865A (en) | 2019-07-11 | 2019-07-11 | A kind of pollen pini active peptide extracting method |
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| CN201910622191.8A CN110229865A (en) | 2019-07-11 | 2019-07-11 | A kind of pollen pini active peptide extracting method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN107574215A (en) * | 2017-07-25 | 2018-01-12 | 赵德润 | A kind of preparation method of pollen pini active peptide |
| CN108486203A (en) * | 2018-05-30 | 2018-09-04 | 烟台新时代健康产业有限公司 | A kind of preparation method of the pollen pini peptide with anti-fatigue active |
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2019
- 2019-07-11 CN CN201910622191.8A patent/CN110229865A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229973A (en) * | 2011-06-15 | 2011-11-02 | 承德畅达生物科技有限公司 | Extraction method of Chinese pine pollen small peptides |
| CN107574215A (en) * | 2017-07-25 | 2018-01-12 | 赵德润 | A kind of preparation method of pollen pini active peptide |
| CN108486203A (en) * | 2018-05-30 | 2018-09-04 | 烟台新时代健康产业有限公司 | A kind of preparation method of the pollen pini peptide with anti-fatigue active |
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