CN110218797B - FGFR gene fusion probe, detection method and kit - Google Patents
FGFR gene fusion probe, detection method and kit Download PDFInfo
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- CN110218797B CN110218797B CN201910680223.XA CN201910680223A CN110218797B CN 110218797 B CN110218797 B CN 110218797B CN 201910680223 A CN201910680223 A CN 201910680223A CN 110218797 B CN110218797 B CN 110218797B
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Abstract
The invention relates to an FGFR gene fusion probe, a detection method and a kit. The invention provides a probe combination for detecting human FGFR gene fusion, which comprises the following probes: FGFR2 (NM _ 001144917): probe coverage of all exons and intron region No. 1, 15,17; FGFR3 (NM _ 000142): probe coverage of all exons, intron 17,18 and the 3' UTR region; probe coverage of intron region 11, 14 of FGFR3 chaperone gene TACC3 (NM — 006342). The invention also provides a kit for detecting FGFR gene fusion, in particular a kit for detecting FGFR gene fusion at one time and an automatic device for analyzing human gene FGFR gene fusion. The invention is suitable for the detection of a wide range of samples.
Description
Technical Field
The invention belongs to the technical field of biological gene detection technology and biological information, and particularly relates to a capture probe design, a high throughput sequencing (NGS) experiment, a data analysis method and a kit for gene fusion detection.
Background
The Fibroblast Growth Factor Receptor (FGFR) family mainly includes 4 types of receptors FGFR1, FGFR2, FGFR3 and FGFR4, and plays an important role in angiogenesis, wound healing and embryonic development. FGFR is a transmembrane protein, with an extracellular domain, a transmembrane domain, and an cytoplasmic tyrosine kinase domain. Binding of FGF ligands to FGFR initiates a cascade of signaling events within the cell.
FGFR gene mutations, including gene fusions, cause a variety of disorders. The gene fusion belongs to one kind of gene variation, and refers to a chimeric gene formed by connecting end to end genes at different positions on two or more chromosomes due to chromosome structure distortion, including deletion, duplication, inversion, translocation and the like, and an expression product of the chimeric gene is a fusion protein. In gene fusion, the sequences of all or a part of two genes are fused to each other to form a novel gene. Gene fusions may be the result of chromosomal translocations, intermediate deletions, or chromosomal inversions. Certain kinases are the driver genes for cancer, and corresponding gene-targeted inhibitors have also been approved, including the inhibitor gleevec for BCR-ABL fusion; the inhibitors for ALK fusion, crizotinib (crizotinib), ai Leti-ni (alectinib), ceritinib (ceritinib), loratinib (loratinib); crizotinib (crizotinib), an inhibitor against ROS1 fusion; larotnectinib, an inhibitor of NTRK fusion. Traditional methods for detecting these gene fusions are usually FISH and IHC, but these fluxes are low and require separate design of kits for individual genes, and fused partner genes are often difficult to detect, thus also having limitations in clinical practice.
High throughput Sequencing technology is also called Next Generation Sequencing (NGS), and can perform Sequencing on hundreds of thousands to millions of molecules in a cellular genome at a time, so that the technology is widely applied to genome research. High-throughput sequencing enables rapid sequencing of a large number of sequences, can be used for gene mutation detection in different individuals, such as tumor patients, and provides beneficial advice and guidance for personalized therapy. NGS provides a high throughput, high accuracy and high sensitivity method for detecting fusion.
Disclosure of Invention
The invention provides a capture probe, a kit and a data analysis method which are used for detecting FGFR1, FGFR2 and FGFR3 gene fusion (especially human gene FGFR2/3 gene fusion) variation with high sensitivity and specificity. The detection kit can be used for various samples, such as tumor tissue biopsy samples, FFPE samples, liquid biopsy plasma, cfDNA and other different types of DNA samples. The method and the kit have high detection sensitivity and specificity and good repeatability, and provide a biomarker screening method for the clinical research of the FGFR inhibitor.
In some embodiments, the invention provides a probe combination for detecting a human gene FGFR1/2/3 gene fusion (in particular a human gene FGFR2/3 gene fusion) comprising probes of the FGFR2-COL14A1, FGFR3-TACC3, FGFR3-BAIAP2L1 fusion site, said probe combination comprising the following probes selected from the attached table one:
aiming at the polynucleotide sequence from the initial site 1729490 to the termination site 1729609 on the chromosome 4 where the human TACC3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 501,
the probe sequence is SEQ ID No. 502 aiming at the polynucleotide sequence from the initial site 1729550 to the termination site 1729669 on the chromosome 4 of the human TACC3 gene or the complementary sequence thereof,
aiming at the polynucleotide sequence from the initial site 1729610 to the termination site 1729729 on the chromosome 4 where the human TACC3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 503,
aiming at the polynucleotide sequence from the initial site 1729670 to the termination site 1729789 on the chromosome 4 where the human TACC3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 504,
the probe sequence is SEQ ID No. 505 aiming at the polynucleotide sequence from the initial site 1729730 to the termination site 1729849 on the chromosome 4 where the human TACC3 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from the initial site 1729790 to the termination site 1729909 on the chromosome 4 where the human TACC3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 506,
aiming at the polynucleotide sequence from the initial site 1808662 to the termination site 1808781 on the chromosome 4 where the human FGFR3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 72,
the probe sequence is SEQ ID No. 79 aiming at the polynucleotide sequence from the initial site 1808782 to the termination site 1808901 on the No. 4 chromosome where the human FGFR3 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 1808722 to a termination site 1808841 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 96,
aiming at the polynucleotide sequence from an initial site 1808692 to a termination site 1808811 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 269,
the probe sequence is SEQ ID No. 292 aiming at the polynucleotide sequence from the initial site 1808752 to the termination site 1808871 on the No. 4 chromosome where the human FGFR3 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from the initial site 1808812 to the termination site 1808931 on the No. 4 chromosome where the human FGFR3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 275,
aiming at the polynucleotide sequence from an initial site 123241115 to a termination site 123241234 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 111,
the probe sequence is SEQ ID No. 158 aiming at the polynucleotide sequence from the initial site 123241175 to the termination site 123241294 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 165 aiming at the polynucleotide sequence from the initial site 123241055 to the termination site 123241174 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from the initial site 123240995 to the termination site 123241114 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 187,
aiming at the polynucleotide sequence from the initial site 123241295 to the termination site 123241414 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 193,
aiming at the polynucleotide sequence from an initial site 123241235 to a termination site 123241354 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 212,
aiming at the polynucleotide sequence from an initial site 123240965 to a termination site 123241084 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 295,
aiming at the polynucleotide sequence from an initial site 123241145 to a termination site 123241264 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 306,
the probe sequence is SEQ ID No. 352 aiming at the polynucleotide sequence from the initial site 123241205 to the termination site 123241324 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from the initial site 123241085 to the termination site 123241204 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 358,
the probe sequence is SEQ ID No. 380 aiming at the polynucleotide sequence from the initial site 123241025 to the termination site 123241144 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123241265 to a termination site 123241384 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 402,
aiming at the polynucleotide sequence from an initial site 123240935 to a termination site 123241054 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 100,
aiming at the polynucleotide sequence from an initial site 123242135 to a termination site 123242254 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 106,
the probe sequence is SEQ ID No. 109 aiming at the polynucleotide sequence from the initial site 123243095 to the termination site 123243214 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123240755 to a termination site 123240874 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 113,
aiming at the polynucleotide sequence from an initial site 123240815 to a termination site 123240934 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 114,
aiming at the polynucleotide sequence from an initial site 123240275 to a termination site 123240394 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 115,
aiming at the polynucleotide sequence from an initial site 123242195 to a termination site 123242314 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 121,
the probe sequence is SEQ ID No. 123 aiming at the polynucleotide sequence from the initial site 123240515 to the termination site 123240634 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123241655 to a termination site 123241774 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 125,
aiming at the polynucleotide sequence from an initial site 123240335 to a termination site 123240454 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 128,
aiming at the polynucleotide sequence from the initial site 123240635 to the termination site 123240754 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 129,
aiming at the polynucleotide sequence from an initial site 123239735 to a termination site 123239854 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 131,
aiming at the polynucleotide sequence from the initial site 123240695 to the termination site 123240814 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 133,
the probe sequence is SEQ ID No. 137 aiming at the polynucleotide sequence from the initial site 123241595 to the termination site 123241714 on the No. 10 chromosome where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123239495 to a termination site 123239614 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 138,
aiming at the polynucleotide sequence from an initial site 123239915 to a termination site 123240034 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 139,
the probe sequence is SEQ ID No. 143 aiming at the polynucleotide sequence from the initial site 123242735 to the termination site 123242854 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242975 to a termination site 123243094 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 148,
aiming at the polynucleotide sequence from an initial site 123240455 to a termination site 123240574 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 149,
aiming at the polynucleotide sequence from an initial site 123240095 to a termination site 123240214 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 150,
the probe sequence is SEQ ID No. 151 aiming at the polynucleotide sequence from the initial site 123241895 to the termination site 123242014 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123240215 to a termination site 123240334 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 153,
the probe sequence is SEQ ID No. 156 aiming at the polynucleotide sequence from the initial site 123242675 to the termination site 123242794 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 157 aiming at the polynucleotide sequence from the initial site 123239975 to the termination site 123240094 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123240575 to a termination site 123240694 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 160,
aiming at the polynucleotide sequence from an initial site 123243035 to a termination site 123243154 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 163,
aiming at the polynucleotide sequence from an initial site 123239855 to a termination site 123239974 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 164,
the probe sequence is SEQ ID No. 167 aiming at the polynucleotide sequence from the initial site 123240155 to the termination site 123240274 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123239795 to a termination site 123239914 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 170,
aiming at the polynucleotide sequence from the initial site 123241775 to the termination site 123241894 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 171,
aiming at the polynucleotide sequence from the initial site 123242915 to the termination site 123243034 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 172,
aiming at the polynucleotide sequence from the initial site 123242615 to the termination site 123242734 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 175,
aiming at the polynucleotide sequence from the initial site 123241955 to the termination site 123242074 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 182,
aiming at the polynucleotide sequence from an initial site 123240875 to a termination site 123240994 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 183,
the probe sequence is SEQ ID No. 186 aiming at the polynucleotide sequence from the initial site 123239555 to the termination site 123239674 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242255 to a termination site 123242374 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 188,
aiming at the polynucleotide sequence from an initial site 123240395 to a termination site 123240514 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 195,
aiming at the polynucleotide sequence from the initial site 123239615 to the termination site 123239734 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 197,
aiming at the polynucleotide sequence from an initial site 123240035 to a termination site 123240154 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 199,
aiming at the polynucleotide sequence from an initial site 123242075 to a termination site 123242194 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 201,
aiming at the polynucleotide sequence from an initial site 123241835 to a termination site 123241954 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 202,
the probe sequence is SEQ ID No. 207 aiming at the polynucleotide sequence from the initial site 123241715 to the termination site 123241834 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242015 to a termination site 123242134 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 208,
aiming at the polynucleotide sequence from an initial site 123242855 to a termination site 123242974 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 209,
the probe sequence is SEQ ID No. 215 aiming at the polynucleotide sequence from the initial site 123242555 to the termination site 123242674 on the No. 10 chromosome where the human FGFR2 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 216 aiming at the polynucleotide sequence from the initial site 123239675 to the termination site 123239794 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242795 to a termination site 123242914 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 217,
aiming at the polynucleotide sequence from an initial site 123242165 to a termination site 123242284 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 301,
the probe sequence is SEQ ID No. 304 aiming at the polynucleotide sequence from the initial site 123243125 to the termination site 123243244 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 308 aiming at the polynucleotide sequence from the initial site 123240785 to the termination site 123240904 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123240845 to a termination site 123240964 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 309,
aiming at the polynucleotide sequence from an initial site 123240305 to a termination site 123240424 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 310,
aiming at the polynucleotide sequence from an initial site 123242225 to a termination site 123242344 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 316,
aiming at the polynucleotide sequence from an initial site 123241685 to a termination site 123241804 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 319,
aiming at the polynucleotide sequence from the initial site 123240365 to the termination site 123240484 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 322,
aiming at the polynucleotide sequence from an initial site 123240665 to a termination site 123240784 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No: 323,
aiming at the polynucleotide sequence from an initial site 123239765 to a termination site 123239884 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 325,
aiming at the polynucleotide sequence from an initial site 123240725 to a termination site 123240844 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 327,
the probe sequence is SEQ ID No. 331 aiming at the polynucleotide sequence from the initial site 123241625 to the termination site 123241744 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
the probe sequence is SEQ ID No. 332 aiming at the polynucleotide sequence from the initial site 123239525 to the termination site 123239644 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123239945 to a termination site 123240064 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 333,
aiming at the polynucleotide sequence from an initial site 123242765 to a termination site 123242884 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 337,
aiming at the polynucleotide sequence from an initial site 123243005 to a termination site 123243124 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 342,
aiming at the polynucleotide sequence from an initial site 123240485 to a termination site 123240604 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No: 343,
the probe sequence is SEQ ID No. 344 aiming at the polynucleotide sequence from the initial site 123240125 to the termination site 123240244 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123241925 to a termination site 123242044 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 345,
the probe sequence is SEQ ID No. 347 aiming at the polynucleotide sequence from the initial site 123240245 to the termination site 123240364 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242705 to a termination site 123242824 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 350,
aiming at the polynucleotide sequence from an initial site 123240005 to a termination site 123240124 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 351,
aiming at the polynucleotide sequence from an initial site 123243065 to a termination site 123243184 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 356,
aiming at the polynucleotide sequence from the initial site 123239885 to the termination site 123240004 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 357,
aiming at the polynucleotide sequence from an initial site 123240185 to a termination site 123240304 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 360,
aiming at the polynucleotide sequence from an initial site 123239825 to a termination site 123239944 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 363,
the probe sequence is SEQ ID No. 364 aiming at the polynucleotide sequence from the initial site 123241805 to the termination site 123241924 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 123242945 to a termination site 123243064 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 365,
aiming at the polynucleotide sequence from an initial site 123242645 to a termination site 123242764 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 368,
aiming at the polynucleotide sequence from an initial site 123241985 to a termination site 123242104 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 375,
aiming at the polynucleotide sequence from an initial site 123240905 to a termination site 123241024 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 376,
aiming at the polynucleotide sequence from the initial site 123239585 to the termination site 123239704 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 379,
aiming at the polynucleotide sequence from the initial site 123240425 to the termination site 123240544 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 386,
aiming at the polynucleotide sequence from an initial site 123239645 to a termination site 123239764 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 387,
aiming at the polynucleotide sequence from the initial site 123240065 to the termination site 123240184 on the chromosome 10 where the human FGFR2 gene is positioned or the complementary sequence thereof, the probe sequence is SEQ ID No. 389,
aiming at the polynucleotide sequence from the initial site 123242105 to the termination site 123242224 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 391,
aiming at the polynucleotide sequence from an initial site 123241865 to a termination site 123241984 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 392,
aiming at the polynucleotide sequence from an initial site 123241745 to a termination site 123241864 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 397,
aiming at the polynucleotide sequence from an initial site 123242045 to a termination site 123242164 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 398,
aiming at the polynucleotide sequence from the initial site 123242885 to the termination site 123243004 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 399,
aiming at the polynucleotide sequence from an initial site 123242585 to a termination site 123242704 on a chromosome 10 where a human FGFR2 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 405,
the probe sequence is SEQ ID No. 406 aiming at the polynucleotide sequence from the initial site 123239705 to the termination site 123239824 on the chromosome 10 where the human FGFR2 gene is located or the complementary sequence thereof,
the probe sequence is SEQ ID No. 407 aiming at the polynucleotide sequence from the initial site 123242825 to the termination site 123242944 on the No. 10 chromosome where the human FGFR2 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 1809043 to a termination site 1809162 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 74,
the probe sequence is SEQ ID No. 78 aiming at the polynucleotide sequence from the initial site 1809163 to the termination site 1809282 on the No. 4 chromosome where the human FGFR3 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 1808983 to a termination site 1809102 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 82,
aiming at the polynucleotide sequence from the initial site 1809283 to the termination site 1809402 on the chromosome 4 where the human FGFR3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 86,
the probe sequence is SEQ ID No. 91 aiming at the polynucleotide sequence from the initial site 1809223 to the termination site 1809342 on the No. 4 chromosome where the human FGFR3 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 92 aiming at the polynucleotide sequence from the initial site 1809103 to the termination site 1809222 on the No. 4 chromosome where the human FGFR3 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 97 aiming at the polynucleotide sequence from the initial site 1809343 to the termination site 1809462 on the No. 4 chromosome where the human FGFR3 gene is located or the complementary sequence thereof,
aiming at the polynucleotide sequence from an initial site 1809193 to a termination site 1809312 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 274,
aiming at the polynucleotide sequence from the initial site 1809013 to the termination site 1809132 on the No. 4 chromosome where the human FGFR3 gene is located or the complementary sequence thereof, the probe sequence is SEQ ID No. 278,
aiming at the polynucleotide sequence from an initial site 1809313 to a termination site 1809432 on a No. 4 chromosome where a human FGFR3 gene is located or a complementary sequence thereof, the probe sequence is SEQ ID No. 282,
the probe sequence is SEQ ID No. 287 aiming at the polynucleotide sequence from the initial site 1809253 to the termination site 1809372 on the chromosome 4 where the human FGFR3 gene is positioned or the complementary sequence thereof,
the probe sequence is SEQ ID No. 288 aiming at the polynucleotide sequence from the start site 1809133 to the end site 1809252 on the chromosome 4 where the human FGFR3 gene is located or the complementary sequence thereof.
In some embodiments, it has been found that the probe combinations of the invention are capable of detecting a broad range of FGFR1/2/3 (especially FGFR 2/3) gene fusions, including in particular, for example, FGFR2-COL14A1, FGFR3-TACC3 and FGFR3-BAIAP2L1 fusions, with high sensitivity and specificity. In some embodiments, it has been found that the probe combinations of the invention are capable of detecting a broad range of FGFR1/2/3 (especially FGFR 2/3) gene fusions, including, inter alia, ZNF198-FGFR1, FGFR2-BICC1 and FGFR3-WHSC1, with high sensitivity and specificity.
In some embodiments, the probe combinations for detecting human gene FGFR1/2/3 (especially FGFR 2/3) gene fusions of the present invention comprise probes selected from one or more of the following fusion genes against FGFR1 selected from the appended table: BAG4-FGFR1, FGFR1OP-FGFR1, FN1-FGFR1, CEP110-FGFR1, TPR-FGFR1, BCR-FGFR1, CPSF6-FGFR1, ERLIN2-FGFR1, FGFR1-TACC1, HERV-K-FGFR1, ACPP-FGFR1, CUX1-FGFR1, FGFR1-ADAM18, FGFR1-ADGRA2, FGFR1-CSMD1, FGFR1-LETM2, FGFR1OP2-FGFR1, FGFR1-TTLL1, FOXO1-FGFR1, LRP 1-FGFR1, MYO18A-FGFR1, NUP98-FGFR1, RAB11FIP1-FGFR1, RANBP2-FGFR1, RHOT 1-SQFGFR 1, STM1-FGFR1, TRIM24-FGFR1 and/or ZNF198-FGFR1.
In some embodiments, the probe combination for detecting FGFR1/2/3 (especially FGFR 2/3) gene fusions of the human genes of the present invention comprises probes selected from the following one or more fusion genes against FGFR2 selected from the accompanying table one: FGFR2-BICC1, FGFR2-KIAA1217, FGFR2-AHCYL1, FGFR2-CCDC6, FGFR2-NOL4, FGFR2-TACC2, FGFR2-KIAA1967, FGFR2-NPM1, FGFR2-NRAP, FGFR2-POC1B, FGFR-ARHGAP 24, FGFR2-CASP7, FGFR2-CIT, FGFR2-KIAA1598, FGFR2-LAMC1, FGFR2-MACF1, FGFR2-OFD1, FGFR2-PAWR, FGFR2-SHTN1, FGFR2-SPERT, FGFR2-TACC1 FGFR2-TACC3, FGFR2-WAC, AHCYL1-FGFR2, CCAR2-FGFR2, CCDC46-FGFR2, CTNNA3-FGFR2, DNM1-FGFR2, FGFR2-ACLY, FGFR2-AFF1, FGFR2-AFF3, FGFR2-AFR, FGFR2-AP1M1, FGFR2-ATF2, FGFR2-BFSP2, FGFR2-C10ORF118, FGFR2-C10ORF68, FGFR2-CBX5, FGFR2-CCAR2, FGFR2-CCDC158, AHCYL1-FGFR2, CCAR 2-ACR 2, and FGFR2-AFR 2 FGFR2-CCDC186, FGFR2-COL16A1, FGFR2-CTNNA3, FGFR2-EIF4A2, FGFR2-ENPP2, FGFR2-ETV6, FGFR2-FAM76A, FGFR-FILIP 1, FGFR2-FRK, FGFR2-GAB2, FGFR2-GOPC, FGFR2-KCNH7, FGFR2-KIF14, FGFR2-KLRAQ1, FGFR2-MGEA5, FGFR2-NCALD, FGFR2-NRBF2, FGFR 2-PPADPDC 1A, FGFR-PPHLN 1, and FGFR2-EIF 2 FGFR2-RABGAP1L, FGFR-RABPGAP 1L, FGFR-RASAL 2, FGFR2-RASSF4, FGFR2-ROCK1, FGFR2-SH3GLB, FGFR2-SHC2, FGFR2-SHROOM, FGFR2-SLMAP, FGFR2-SMN1, FGFR2-SOGA1, FGFR2-SORBS1, FGFR2-SPICE1, FGFR2-STRN4, FGFR2-TFEC, FGFR2-TNS1, FGFR2-TRA B, FGFR-TTC 28, FGFR2-USP10, FGFR2-VCL and/or FGFR2-ZYMY4.
In some embodiments, the probe combinations for detecting human gene FGFR1/2/3 (especially FGFR 2/3) gene fusions of the present invention comprise probes selected from the following one or more fusion genes against FGFR3 selected from the appended table: FGFR3-TACC3, FGFR3-BAIAP2L1, TACC3-FGFR3, FGFR3-JAKMIP1, FGFR3-TNIP2, FGFR3-WHSC1, FAM193A-FGFR3, FGFR3-ADD1, FGFR3-AES, FGFR3-AMBRA1, FGFR3-ELAVL3, FGFR3-FBXO28, FGFR3-MLLT10 and/or TEL/ETV6-FGFR3.
In some embodiments, the present invention provides a method of enriching for human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion targets, the method comprising: extracting nucleic acids from a sample of a subject, constructing a nucleic acid library, and capturing human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion targets using the probe combinations described herein, thereby enriching for human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion targets.
In some embodiments, the methods of the invention can be used for a variety of samples, such as tissue biopsy samples, FFPE samples, DNA samples, and liquid biopsy plasma and cfDNA samples. In some embodiments, the subject is not particularly limited and may include, for example, a normal healthy subject, a subject suspected or diagnosed as having a disease, such as cancer. In some embodiments, the cancer related to the present invention is not particularly limited, and may be any cancer (including early, intermediate and late stage cancers) related to FGFR gene mutation (especially gene fusion), including, for example, solid tumors such as intrahepatic bile duct cancer and urothelial cancer, and the like, and various sample sources such as late stage (iiib-iv) intrahepatic bile duct cancer, and FGFR1/FGFR2/FGFR3 gene variation in circulating tumor DNA (ctDNA) extracted from, for example, neutral formalin-fixed paraffin-embedded (FFPE) tissue samples and plasma samples.
In some embodiments, the invention provides kits for detecting a human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion comprising a capture reagent comprising a probe combination described herein. In some embodiments, the kits may include various reagents used in the methods of enriching for human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion targets described herein.
In some embodiments, the human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion can include FGFR2-COL14A1, FGFR3-TACC3, and FGFR3-BAIAP2L1.
In some embodiments, the kit further comprises one or more of: 1) a library preparation reagent such as DNA polymerase, ligase, buffer and/or quality control, 2) a capture reagent other than a probe such as a blocking agent, capture buffer and/or washing solution, 3) a solid support such as a magnetic bead, and/or 4) a high throughput sequencing reagent.
As understood by those skilled in the art, the term "probe" refers to a polynucleotide sequence of variable length that can be used for hybridization detection of identical, similar, or complementary nucleic acid sequences. The sequence used as detection probe may be labeled with a labeling group known in the art, such as a radioactive group, a fluorescent group, a chemiluminescent group, or an electrochemiluminescent group. Polynucleotide sequences are "complementary" in the sense that a sufficient number of matched base pairs in the sequence specifically hybridize to the target sequence being amplified or detected, and sequence complementarity does not necessarily require 100% matched base pairs. Thus, probes described herein encompass corresponding sequences that are not 100% paired with a target sequence, including, for example, sequences that are at least 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% identical to the target sequence or its complement. In some embodiments, the probes described herein encompass, for example, sequences that hybridize to a target sequence under stringent conditions. The term "hybridizes under stringent conditions" under conditions which allow hybridization and washing to hybridize under said conditions to each other of sequences which are at least 60% (65%, 70%, preferably 75%) identical to each other. Such stringent conditions are known to those skilled in the art and may, for example, refer to hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45 ℃ followed by one or more washes with 0.2X SSC,0.1% SDS at 50-65 ℃. The term "hybridization" refers to the formation of complementary A-T and C-G base pairs between two polynucleotide sequences.
In some embodiments, the invention provides an automated apparatus for analyzing human gene FGFR1/2/3 (especially FGFR 2/3) gene fusions. In some embodiments, the automated device may comprise a library capture device that captures human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion targets via a probe combination described herein. In some embodiments, the automated device may further comprise a high throughput sequencing device and/or a result analysis device. In some embodiments, the high throughput sequencing device is configured to determine the sequence of a human gene FGFR1/2/3 (in particular FGFR 2/3) gene fusion target captured by the library capture unit. In some embodiments, the result analysis device comprises:
a processor;
a memory having instructions stored thereon that, when executed by the processor, perform one or more of the following:
1) Obtaining human gene FGFR1/2/3 (especially FGFR 2/3) gene fusion sequencing data determined by the high-throughput sequencing device,
2) The following sequencing sequences were defined as inconsistent reads (discordant reads): the size of the insert is larger than 1000bp, or a pair of reads (reads) of double-end reads (pair end reads) are aligned on different chromosomes, or the direction of the alignment result is incorrect,
3) Defining that the CIGAR character string containing H and S in all the alignment results is a clipped read,
4) Extract all inconsistent reads (discordant reads) and their surrounding clip reads (clipped reads) and mark the source,
5) The above-mentioned reading is subjected to de-duplication,
6) Extracting 27-mers from the above results which are derived from reads of the same chromosome,
7) Filtering common 27-mers and blacklists of 27-mers, typically fused false-positive repeats on chromosomes
8) The reads obtained in the previous step, which contain the above 27-mers, are clustered using a merge algorithm, labeled as clusters (clusters)
9) Fermi-lite was used for local assembly,
10 ) re-aligning to the genome while filtering to retain only the candidate structural variation of the same chromosome,
11 Repeat 6) -9) steps for inconsistent reads (discordant reads) originating from different chromosomes and clipped reads (clipped reads) around them, preserving the candidate structural variations of the different chromosomes,
12 Combine the two results, filter the results that support few reads (support reads), and annotate.
Drawings
Fig. 1 is an example of a probe design method, wherein fig. 1a is an example of FGFR2 intron probe design: higher capture efficiency was achieved by designing 4x tilling probe coverage in the intron region; fig. 1b is a graph of improving the accuracy of FGFR3 fusion detection by designing probes in exon and intron regions of the fusion partner gene TACC 3.
Figure 2 shows the overall flow of an exemplary experiment.
Fig. 3 shows an exemplary bioinformation analysis flow.
Detailed Description
The invention aims to provide a probe sequence for detecting FGFR1/2/3 gene fusion and a distribution mode. The invention designs a specific primer aiming at FGFR1/2/3 gene, and finds that the primer can detect broad FGFR1/2/3 gene fusion with high sensitivity and specificity, particularly common FGFR1/2/3 gene fusion. In a further embodiment of the invention, the primer combination designed by the invention covers all FGFR1/2/3 gene fusions, thereby achieving the purpose of detecting all possible FGFR1/2/3 gene fusions at one time.
A target gene:
FGFR1 (NM _ 001174063): all exons and intron 4,5,7,9, 17;
FGFR2 (NM _ 001144917): all exons and introns No. 1, 15, 17;
FGFR3 (NM _ 000142): all exons as well as intron 17,18 and UTR 3';
probe coverage of intron region 11, 14 of FGFR3 chaperone gene TACC3 (NM — 006342).
Local area range: introns preceding the exon where the target gene portion occurs first in all possible fusion forms on the COSMIC.
Length of the probe: 120 nt, for the area with the length larger than the length of the probe, the target area is completely covered by adopting a probe overlapping mode, and the overlapping area of the probe is 60 nt. For regions of length less than the probe length, the target region is completely covered with a single probe, with the center position of the probe coinciding with the center position of the region. For the fusion hotspot region, higher capture efficiency was achieved by designing 4x tilling probe coverage in the intron region. Each probe was aligned to the whole genome by BLAST; performing score calculation on hit with the length of more than 60; each probe was scored for total according to the number of hit, with higher scores being less specific for the probe. Probes with a score greater than 2 were not considered. Probes in a single area a probe set with the lowest sum of all probe scores in the possible probe combinations for that area is identified. Regions of homologous repeats (e.g., SINE, LINE, LTR, etc.) were excluded from consideration for designing probes. For the genomic repeat region where FGFR is fused, it is difficult to directly design probes, and the accuracy of FGFR3 fusion detection is improved by designing probes in the exon and intron regions of a fusion partner (partner) gene, such as TACC 3. The appropriate probe combination was selected and verified and shown in the attached table one. An example of a probe design method is shown in figure 1.
The invention also provides a kit for detecting FGFR1/2/3 gene fusion, in particular to a kit for detecting FGFR1/2/3 gene fusion at one time. The kit is mainly used for detecting gene DNA variation, and exemplary components of the kit are shown in the table below.
Kits are exemplary compositions (a kit may include one or more reagents, described below, and may be placed in one or more packages).
The method of the invention may comprise one or more of the following stages (and may include for each stage the corresponding reagents suitable for use in that stage, which may be selected by one skilled in the art and may include various commercial reagents or kits): nucleic acid extraction, library construction, library capture, computer sequencing and result analysis. The overall scheme of an exemplary experiment is shown in figure 2. Specific exemplary steps are detailed below:
(1) Extracting the double-stranded nucleic acid of the genome DNA in blood and tissue samples;
(2) Performing denaturation treatment on the DNA double-stranded nucleic acid in the step (1) to obtain a DNA single strand, and performing end repair, linkage and PCR amplification to build a library;
(3) After pooling, capturing said DNA library using the capture probe of claim;
(4) Performing on-line detection on an applicable machine type according to the requirements of the general kit for the sequencing reaction;
(5) And (3) automatically processing the sequenced data, removing background noise, and detecting the gene fusion of the sample according to the bioinformatics analysis method of the gene fusion.
The kit can theoretically detect the fusion of all known and unknown chaperone genes including FGFR1/2/3 genes, and the representative gene fusion information is shown in the table below.
The invention relates to a probe, a method and a product for detecting FGFR1/2/3 representative gene fusion.
The invention also provides a bioinformatics analysis method for detecting FGFR1/2/3 gene fusion (including one-time detection of FGFR1/2/3 gene fusion), and a specific exemplary method is shown in figure 3.
The present invention has been shown to advantageously have one or more of the following effects:
1) The capture probe captures a coding exon region and an exon intron handover region of a genome target gene FGFR1/2/3 region of a human genome, and the specificity is high; reducing non-specific capture;
2) The initial amount of the sample is low, and the sensitivity is high; the gene mutation with the content as low as 2 percent in a 50ng fragmented DNA sample or the gene mutation with the content as low as 0.7 percent in a plasma sample can be detected;
3) The fusion method can be suitable for all FGFR1/2/3 gene fusions at present, does not limit the upstream or downstream of the fusion partner gene and which partner gene is, and can find unknown fusion breakpoints of known partner genes or completely unknown fusion types;
4) The sample types used are wide, including but not limited to solid tumor patients with neutral Formalin Fixed Paraffin Embedded (FFPE) tissue samples and plasma free DNA (cfDNA) extracted from plasma samples, which includes Circulating tumor DNA (ctDNA).
The following describes the test method of the invention in an exemplary manner in each of its five phases: nucleic acid extraction, library construction, library capture, computer sequencing and result analysis.
A. Nucleic acid extraction
1.1 Extracting and purifying the blood plasma sample nucleic acid by adopting a nucleic acid extraction kit according to the manufacturer instructions (recommending a nucleic acid extraction reagent, and details are shown in table 2), wherein the extracted blood plasma free DNA is hereinafter referred to as cfDNA (cell-free DNA);
1.2 Accurately quantitating the extracted cfDNA (it is recommended to use a Qubit fluorescence quantifier); (after the completion of this step, the experiment was interrupted and the extract was stored at-20. + -. 5 ℃ for a long period of time below-70 ℃)
B. Library construction
2. Preparation before experiment
2.1 Taking out the tail end repairing buffer solution, unfreezing at normal temperature, and placing the tail end repairing enzyme in an ice box for later use;
2.2 Preparing a sufficient amount of freshly prepared 80% ethanol for use (the total amount of freshly prepared 80% ethanol is prepared in 1mL per sample, e.g. 12 samples are pooled to prepare 12mL of 80% ethanol);
2.3 gDNA fragmentation (FFPE sample only): taking 50-500 ng gDNA into a 1.5ml centrifugal tube, supplementing less than 50 mu L of nuclease-free water to 50ul, and centrifuging for a short time after mixing uniformly; transferring 50 muL gDNA solution into a breaking pipe and placing the breaking pipe into an interrupting instrument, and selecting an interrupting program with the fragment length of 200-300bp to carry out gDNA fragmentation; (after the step is finished, the experiment can be interrupted, and the product is frozen at 20 +/-5 ℃ and stored for a long time below 70 ℃;
3. end repair
3.1 Adding the following components into a 0.2ml nuclease-free PCR reaction tube or an eight-connected tube (when a large number of samples exist, a mixed solution can be prepared for split charging);
3.2 Shaking and mixing, centrifuging for a short time, and running the following programs on a PCR instrument (the negative quality control substances and the positive quality control substances are synchronously operated with the sample from the step):
4. connection of
4.1 Taking out the reaction tube in the previous step, slightly shaking, uniformly mixing, centrifuging for a short time, and adding the following components into the reaction tube:
4.2 Shaking and mixing, and running the following program on a PCR instrument after short-time centrifugation:
4.3 Taking out the magnetic bead A, and balancing for 30min at room temperature for later use;
4.4 Taking out the reaction tube, adding 43 mu L of magnetic beads A, fully blowing and beating, transferring into a 1.5ml centrifuge tube, and incubating at room temperature for 5min;
4.5 Transferring the centrifugal tube to a magnetic frame, standing for 2min, and carefully sucking and removing supernatant after the solution is clarified;
4.6 Adding 200 μ L of 80% ethanol, covering the centrifugal tube cover, standing at room temperature for 30s, and carefully sucking off the supernatant;
4.7 Repeating the operation for 4.6 times to ensure that no liquid residue exists on the tube wall and the tube bottom;
4.8 Keeping the centrifuge tube on a magnetic frame, and drying at room temperature for 5min (the magnetic beads cannot be dried excessively);
4.9 Adding 21 mu L of enzyme-free water, shaking and resuspending, and standing at room temperature for 5min;
4.10 Transferring the centrifugal tube to a magnetic frame, standing at room temperature for 2min, and sucking 20 mu L of supernatant into a new 0.2ml PCR reaction tube;
5.PCR amplification
5.1 Selecting and recording corresponding sample labels, respectively and independently adding 5 mu L of sample labels into the PCR reaction tube, and marking;
5.2 Add 25. Mu.L of PCR reaction solution I, mix well, run the following program on the PCR instrument after a short centrifugation:
5.3 Taking out the PCR reaction tube, adding 50 mu L of magnetic beads A, fully blowing and beating, transferring into a 1.5ml centrifuge tube, uniformly mixing, and incubating at room temperature for 5min;
5.4 Transferring the centrifuge tube to a magnetic frame, standing at room temperature for 2min, and carefully sucking and removing the supernatant;
5.5 Adding 200 μ L80% ethanol, standing at room temperature for 1min, carefully sucking off the supernatant;
5.6 Repeating the operation for 5.5 times to ensure that no liquid residue exists on the tube wall and the tube bottom;
5.7 Keeping the centrifuge tube on a magnetic frame, and drying at room temperature for 5min (the magnetic beads cannot be dried excessively);
5.8 Adding 31 mu L of enzyme-free water, shaking for resuspension, and standing at room temperature for 5min;
5.9 Transferring the centrifuge tube to a magnetic frame, standing at room temperature for 2min, and sucking the supernatant into a new centrifuge tube of 1.5 ml;
5.10 accurate quantification of library nucleic acids (a Qubit fluorescence quantifier is recommended); (after the step is finished, the experiment can be interrupted, and the product can be stored at the temperature of minus 20 +/-5 ℃ for a long time below minus 70 ℃).
C. Library Capture
6. Capture
6.1 The DNA library was metered and mixed into a new 1.5ml centrifuge tube as follows:
1) Recommending that libraries of four different sample labels are mixed into a mixed library;
2) Libraries with similar concentrations are suggested to be arranged in a mixed library to avoid differences in hybridization efficiency;
3) The total amount of one mixed library is 1.6 mug, and the amount of each library is evenly distributed (if the number of samples is less than 4, the total amount of the mixed libraries is ensured to be 1.6 mug); respectively adding 5 mu L of sealant I and 2 mu L of sealant II into each mixed library, fully mixing, centrifuging for a short time, sealing the opening of a centrifugal tube by using a sealing film, pricking 5 small holes on the sealing film by using a clean gun head, putting into a vacuum concentration instrument, and vacuum-drying until no liquid residue exists;
6.2 Sequentially adding 8.5 muL of capture buffer solution, 2.7 muL of capture reinforcing agent and 3.8 muL of enzyme-free water into the drained mixed library, fully shaking and uniformly mixing, centrifuging for a short time, standing for 10min in a dark place at room temperature (shaking and centrifuging once during 5 min), and redissolving;
6.3 Transferring the liquid into a 0.2ml PCR reaction tube, and placing the tube in a PCR instrument for denaturation at 95 ℃ for 10min;
6.4 Taking out the PCR reaction tube, adding 2 μ L capture probe quickly, mixing well, centrifuging briefly, and incubating overnight at 65 deg.C in PCR instrument (overnight time is no more than 18 hr, hot cover set at 75 deg.C);
6.5 Taking a certain amount of magnetic bead washing liquid (2X), washing liquid I (10X), washing liquid II (10X), washing liquid III (10X) and washing liquid IV (10X), calculating the required amount as shown below, and diluting the required amount to the corresponding 1X working solution by using non-enzyme water for later use;
6.6 Taking a certain amount (calculating the required amount according to 100 mu L multiplied by the number of the mixed libraries) of 1 multiplied by washing liquid I and all 1 multiplied by washing liquid II from the diluted 1 multiplied by working solution in advance, and incubating for more than 2 hours at 65 ℃ in a metal bath for later use;
6.7 Taking the magnetic bead B in advance, and standing for 15-30min at room temperature;
6.8 Immediately taking out a certain amount (calculating the required amount according to 100 mu L multiplied by the number of the mixed libraries) of the magnetic beads B after shaking and resuspending to a new 1.5mL centrifuge tube, transferring to a magnetic frame, standing for 1min, and absorbing and discarding the supernatant;
6.9 Adding a 2-time volume of 1 multiplied by magnetic bead washing solution, fully shaking and resuspending, transferring to a magnetic frame after short-time centrifugation, standing for 1min, and sucking and discarding the supernatant;
6.10 Repeat 6.9 times;
6.11 Adding 1 times of volume of 1 times of magnetic bead washing solution, shaking for resuspension, and subpackaging into different 0.2mL PCR reaction tubes according to 100 mu L per tube;
6.12 Transferring the PCR reaction tube to a magnetic frame with the specification of 0.2ml, standing for 1min, carefully sucking and discarding supernatant to avoid the pipette tip from sucking magnetic beads;
6.13 Taking out the PCR reaction tube after overnight hybridization, transferring all the hybridization products into the PCR reaction tube containing the magnetic beads B, fully and uniformly mixing by vortex, then placing the mixture after short centrifugation on a PCR instrument, incubating the mixture for 45min at 65 ℃, and uniformly mixing by vortex once every 10min to avoid the magnetic beads from settling to the bottom;
6.14 After incubation, adding 100 mu L of 1 multiplied washing solution I preheated at 65 ℃ into each tube, transferring all the suspension into a 1.5mL centrifuge tube (placed on a 65-degree metal bath 5 minutes in advance), shaking and uniformly mixing, centrifuging for a short time, transferring to a magnetic frame, standing for 5s, and sucking and removing supernatant;
6.15 Adding 200 μ L of 1 × washing solution II preheated at 65 deg.C, shaking, mixing, centrifuging for a short time, incubating at 65 deg.C for 5min, transferring to magnetic frame, standing for 5s, and removing supernatant. Repeating the step once;
6.16 Adding 200 μ L of 1 × washing solution I, vortexing for 2min, centrifuging for a short time, standing on a magnetic frame, and removing the supernatant after magnetic beads are completely separated;
6.17 Adding 200 mu L of 1 Xwashing liquid III, whirling for 1min, centrifuging for a short time, standing on a magnetic frame, and removing the supernatant after magnetic beads are completely separated; adding 200 mu L of 1 Xwashing liquid IV, whirling for 30s, centrifuging for a short time, standing on a magnetic frame, and removing the supernatant after magnetic beads are completely separated;
6.18 Adding 18 mu L of enzyme-free water, shaking, resuspending, and transferring to 0.2mL PCR reaction tube;
6.19 Respectively adding 25 mul of PCR reaction solution and 5 mul of PCR primers into the PCR reaction tube, fully mixing, centrifuging for a short time, and then running the following programs on a PCR instrument:
6.20 And taking the magnetic bead A out of a four-degree refrigerator, and balancing for 30min at room temperature for later use. Taking out the PCR reaction tube after the PCR is finished, and transferring all products into a new 1.5mL centrifuge tube after the PCR reaction tube is centrifuged for a short time;
6.21 Adding 75 mu L of magnetic bead A, shaking and uniformly mixing, standing at room temperature for 10min, then centrifuging briefly, transferring to a magnetic frame, standing for 1min, and absorbing and removing the supernatant;
6.22 200 μ L of 80% ethanol was added, and after standing for 30 seconds, the supernatant was aspirated. Repeating the step once;
6.23 Opening the cover and drying until no liquid drops remain and the magnetic beads do not reflect light;
6.24 Adding 22 mu L of enzyme-free water, shaking the resuspended magnetic beads, carrying out short-time centrifugation, standing at room temperature for 5min, transferring to a magnetic frame, standing for 1min, and sucking 20 mu L of supernatant into a new 1.5mL centrifuge tube for later use;
6.25 1 μ L of the capture library was taken for accurate quantification (a Qubit fluorescence quantifier was recommended); (after the completion of this step, the experiment was interrupted, and the product was frozen at-20. + -. 5 ℃ C. And stored at-70 ℃ C. For a long period of time.)
D. Sequencing on computer
7. Sequencing on machine
7.1 Performing on-line detection on an applicable machine type according to the requirements of a general kit (sequencing method) for sequencing reaction (self-contained reagents, see table 2 for details);
E. analysis of results
8. Result acquisition and analysis
And transmitting the sequenced data to human FGFR1/2/3 mutation joint detection analysis software, and operating the software to perform comparison analysis to obtain a sample mutation result. The letter generation analysis process comprises the following specific steps:
8.1 The following sequencing sequences were defined as inconsistent reads: the size of the insert fragment is more than 1000bp, or a pair of reading numbers of the double-end reading numbers are aligned on different chromosomes, or the direction of the alignment result is incorrect,
8.2 Defining the CIGAR character string containing H and S in all the alignment results as a clipping reading
8.3 Extracting all inconsistent readings and their surrounding clipped readings and marking the source;
8.4 Removing the weight of the above readings
8.5 Extracting 27-mers from the same chromosome from the above results
8.6 Filtering common 27-mers and blacklisted 27-mers
8.7 The reading obtained in the last step and containing the 27-mer is clustered (cluster) by using a union-find algorithm, and the reading is marked as a cluster
8.8 Local Assembly Using fermi-lite software
8.9 Realigning to genome, and retaining only the alternate structural variation of the same chromosome
8.10 Repeating 8.5-8.9 steps for the inconsistency and clipping reading of different chromosomes in source, and retaining the alternate structural variation of different chromosomes
8.11 Combining, filtering and annotating the two results;
8.12 Reference genome and database, the current reference genome of the letter generation analysis process is based on USCS hg19, version No.: v1.0 (20-Mar-2009 version). The database includes:
1) Reference genome and common SNP 27-mer data files within a panel
2) 27-mer data files introduced by Experimental and sequencing Processes
3) Fusing the annotation files: GTF file converted by UCSC hgTables 2013-06-14 edition
9. And (3) judging the analysis result of the fusion data:
9.1 The analysis parameters were set as follows:
10. performance comparison analysis of fusion software:
10.1 Sample selection and experimental procedure:
the 75x2 samples tested using UDI were 10 in total, and three tools were each called SV. And (3) filtering conditions: autofusion takes the existing conditions. delly2 uses PASS and presise tag as filtering criteria, and requires a breakpoint distance of greater than 1000 at SV of DEL, INV type, and annotated filtering criteria are consistent with autofusion. BreakID does not require filtering conditions;
10.2 The experimental process comprises the following steps:
the downloaded version of the BreakID cannot be compiled, the header file and the compiling conditions can be modified and part of the code can be run after being modified, but all samples have no result. The final result is that all the filtered results are combined and read by IGV, and then the result is considered to be a true positive result; analysis of why BreakID was not positive: this tool relies on the SA tag of bwa mem; SA tags are required at two ends of the breakpoint, so that the requirement on the bwa version is very likely to exist, and the method is not compatible with the existing process;
10.3 The experimental results are as follows:
true positives 8, with 100% sensitivity and PPV for the current autofusion:
11. limit of detection (LOD) analysis
Using the FGFR1/2/3 fusion probe sequences covered by the targeted capture panel described in the attached Table, assays were performed using two cell lines, wherein A had FGFR2-COL14A1 and FGFR3-TACC3 fusions, and the other B had FGFR3-BAIAP2L1 fusions. The mixed gradients are shown below, with 15-20 samples of each gradient being unequal. Gradient concentrations were quantified by digital PCR:
the current fusion LOD is about 0.7% based on a 95% detection rate, and the detailed results are shown in the following table:
the specificity and sensitivity of the probe combination and the method for detecting FGFR gene fusion are obviously higher than those of the existing methods and probe combinations.
The detection limit performance of the present invention and prior art for detecting gene fusion is shown in the following table:
| product name | Production plant Home-use | Fusion gene of claim Due to the fact that | Detection limit performance index |
| Human 10 gene mutation joint detection kit (reversible end termination sequencing method) | Xiamen moxa De Er | ALK，ROS1,RET | The detected fusion of 30ng DNA sample is 1% of variation abundance |
| Human EGFR/ALK/BRAF/KRAS gene mutation joint detection kit (reversible terminal) End-stop sequencing method | Guangzhou burner Stone (stone) | ALK | The ALK fusion detection limit detected by 50ng of DNA sample is 10 percent of variation abundance |
| EGFR/ALK/ROS1/BRAF/KRAS/HER2 gene mutation detection kit (reversible) End-stop sequencing method） | Nanjing world And | ALK，ROS1 | ROS1 fusion detected by 250ng DNA sample is 1% of variation abundance, and ALK fusion is variation The abundance ratio is 2.5% |
| Human EGFR, KRAS, BRAF, PIK3CA, ALK, ROS1 gene mutation detection kit (semi-half) Conductor sequencing method | Tianjinuo Grain (standing grain) | ALK，ROS1 | 30ng of DNA detected with a fusion detection limit of 100 copies |
| Human FGFR1/2/3 gene mutation joint detection kit (high-throughput sequencing method) (the invention) Invention) | Idea di | FGFR1,FGFR2, FGFR3 | Detection limit of FGFR fusion detected by 30-60ng cfDNA or 50-200 ng gDNA The variation abundance is 0.7 percent |
In conclusion, the invention provides a set of fusion capture probes for detecting human genes FGFR1, FGFR2 and FGFR3 by high-throughput sequencing, a detection method, a kit and application thereof, the performance is greatly improved, and the detection result shows that the detection system can accurately detect fusion variation of FGFR1/2/3 genes at one time, and the detection limit can be as low as 0.7%. Therefore, the method can also be widely applied to the detection of various samples, including tumor tissue biopsy samples, FFPE samples, DNA samples, liquid biopsy plasma and cfDNA samples.
Attached table-attached FGFR1/2/3 fusion probe sequence for targeted capture of panel coverage
Note: the genome data version number is UCSC hg19, and the corresponding genome version Genbank number is GCA _000001405.1.
The exemplary embodiments of the present invention are illustrated by the above embodiments. The embodiments do not limit the technical solutions of the present invention. Those skilled in the art will understand that: modifications may be made to the above-described embodiments, or equivalents may be substituted for some or all of the features thereof. Such modifications or substitutions are intended to be within the scope of the present invention.
Sequence listing
<110> Shanghai thought Di medical laboratory Co., ltd
<120> FGFR gene fusion probe, detection method and kit
<130> IB191545
<160> 624
<170> PatentIn version 3.3
<210> 1
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 1
cttgtcagag ggcaccacag agtccattat gatgctccag gtggcataac ggacctgagg 60
ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 120
<210> 2
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 2
ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 60
ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 120
<210> 3
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 3
ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 60
gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 120
<210> 4
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 4
gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 60
ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 120
<210> 5
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 5
ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 60
ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 120
<210> 6
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 6
ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 60
acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 120
<210> 7
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 7
acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 60
catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 120
<210> 8
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 8
catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 60
aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 120
<210> 9
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 9
aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 60
actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 120
<210> 10
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 10
actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 60
atgtagtgag tgcatggtga attaatttaa aatatttctg gctgggtgcg gtggctcaag 120
<210> 11
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 11
gtgagactcc gtctaaaaaa aaaaaaaaaa tgcttacacc ctttaagtta gtaatttcac 60
ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 120
<210> 12
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 12
ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 60
cattatttat tatggtgaaa aaccagaaaa tacctaatat cacaaaatag ttcattaaat 120
<210> 13
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 13
gcccagccaa attatgggat tttaaaataa tggaatctta tacagtcatg aaaaattatg 60
tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 120
<210> 14
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 14
tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 60
aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 120
<210> 15
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 15
aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 60
gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 120
<210> 16
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 16
gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 60
gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 120
<210> 17
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 17
gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 60
ggcctcccct gttcccatta ctctaacttt cgcatgcaca cacacgtacc ttgtagcctc 120
<210> 18
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 18
acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 60
ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 120
<210> 19
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 19
ctgtgcctgg cctatggccc cttctcttaa tcaccactca ctcacaactt cagtctgaag 60
taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 120
<210> 20
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 20
acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 60
agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 120
<210> 21
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 21
ccactgcgcc cggccttctt tcccttttac aagtacagaa caagtggact tcaaaagtcc 60
aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 120
<210> 22
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 22
ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 60
accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 120
<210> 23
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 23
cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 60
gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 120
<210> 24
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 24
cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 60
gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 120
<210> 25
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 25
acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 60
accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 120
<210> 26
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 26
ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 60
atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 120
<210> 27
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 27
tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 60
acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 120
<210> 28
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 28
acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 60
ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 120
<210> 29
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 29
accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 60
ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 120
<210> 30
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 30
ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 60
cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 120
<210> 31
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 31
taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 60
ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 120
<210> 32
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 32
tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 60
ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 120
<210> 33
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 33
gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 60
gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 120
<210> 34
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 34
gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 60
tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 120
<210> 35
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 35
atctctttgt cggtggtatt aactccagca gtctagaaga gacaacggaa gcaaaatgga 60
caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 120
<210> 36
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 36
tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 60
gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 120
<210> 37
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 37
tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 60
aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 120
<210> 38
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 38
gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 60
accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 120
<210> 39
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 39
gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 60
cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 120
<210> 40
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 40
tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 60
agactggtgc catgattacc ttcaagatct ggacataagg caggttgtct gggccaatct 120
<210> 41
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 41
accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 60
gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 120
<210> 42
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 42
acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 60
agggaatgcc taaccccttc tctctctttt ttaagagatg gggtaccatt ctgtcaaccc 120
<210> 43
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 43
tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 60
acagtgtctc acgcatacgg tttggtttgg tgttatctgt ttctttctcc tctgaagagg 120
<210> 44
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 44
ctgcccgggg aaagccaaga gagacaggca gggtggagag gagcagctgg tcaggctcag 60
gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 120
<210> 45
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 45
ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 60
ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 120
<210> 46
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 46
atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 60
ccatctactt tctgttacct gtctgcgcag agggatgctc ttggccagct tgtgcacagc 120
<210> 47
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 47
ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 60
tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 120
<210> 48
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 48
agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 60
tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 120
<210> 49
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 49
atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 60
acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 120
<210> 50
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 50
gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 60
gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 120
<210> 51
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 51
tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 60
gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 120
<210> 52
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 52
tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 60
tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 120
<210> 53
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 53
gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 60
tcccgtgggg aagggtgggc acacagggga caagggtatg gtatggctga gatggtttgg 120
<210> 54
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 54
caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 60
gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 120
<210> 55
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 55
accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 60
acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 120
<210> 56
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 56
tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 60
attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 120
<210> 57
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 57
cagcagtgtg aaaatggact aatacaaaca gttctcttct ctaccctgca tctctgcact 60
tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 120
<210> 58
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 58
tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 60
acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 120
<210> 59
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 59
ggccgaacca gaagaacccc agagttcatg gatgcactgg agtcagcaga cacctgcaag 60
gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 120
<210> 60
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 60
ctgtgatggg cgagaggaag cagcgatggg ccgggcccct cctccctgct cagggaggtg 60
cgtgcacgca gtggggacgg cctgagctct ggctctggca cgggcagcct tacctggttg 120
<210> 61
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 61
gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 60
acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 120
<210> 62
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 62
ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 60
ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 120
<210> 63
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 63
ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 60
tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 120
<210> 64
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 64
aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 60
tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 120
<210> 65
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 65
gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 60
gatttattac ctttcccttt tagttttttt tttttttttt ttttttaacg cagagtctca 120
<210> 66
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 66
aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 60
tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 120
<210> 67
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 67
gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 60
tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 120
<210> 68
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 68
gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 60
tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 120
<210> 69
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 69
attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 60
atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 120
<210> 70
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 70
aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 60
ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 120
<210> 71
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 71
tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 60
aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 120
<210> 72
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 72
gtgagtgctg gctctggcct ggtgccaccc gcctatgccc ctccccctgc cgtccccggc 60
catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 120
<210> 73
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 73
ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 60
ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 120
<210> 74
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 74
tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 60
ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 120
<210> 75
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 75
tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 60
tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 120
<210> 76
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 76
cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 60
ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 120
<210> 77
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 77
ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 60
cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 120
<210> 78
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 78
tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 60
caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 120
<210> 79
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 79
agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 60
ggagtacctg gacctgtcgg cgcctttcga gcagtactcc ccgggtggcc aggacacccc 120
<210> 80
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 80
acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 60
gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 120
<210> 81
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 81
ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 60
atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 120
<210> 82
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 82
gacgtgaagg gccactggtc cccaacaatg tgaggggtcc ctagcagccc accctgctgc 60
tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 120
<210> 83
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 83
ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 60
tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 120
<210> 84
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 84
gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 60
ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 120
<210> 85
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 85
tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 60
ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 120
<210> 86
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 86
cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 60
ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 120
<210> 87
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 87
tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 60
tttgttaacc cagcgacgaa ctttccgaaa aataaagaca cctggttgct aacctggccc 120
<210> 88
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 88
ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 60
acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 120
<210> 89
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 89
atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 60
gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 120
<210> 90
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 90
acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 60
ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 120
<210> 91
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 91
caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 60
cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 120
<210> 92
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 92
ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 60
tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 120
<210> 93
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 93
ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 60
tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 120
<210> 94
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 94
gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 60
aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 120
<210> 95
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 95
aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 60
ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 120
<210> 96
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 96
catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 60
agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 120
<210> 97
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 97
ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 60
acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 120
<210> 98
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 98
ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 60
tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 120
<210> 99
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 99
aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 60
tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 120
<210> 100
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 100
caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 60
agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 120
<210> 101
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 101
gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 60
cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 120
<210> 102
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 102
ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 60
cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 120
<210> 103
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 103
aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 60
gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 120
<210> 104
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 104
gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 60
aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 120
<210> 105
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 105
ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 60
ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 120
<210> 106
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 106
tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 60
tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 120
<210> 107
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 107
tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 60
tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 120
<210> 108
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 108
cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 60
tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 120
<210> 109
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 109
agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 60
ctacgcatgt ctcacaagac aaccaaggac aaggggcttc tagaaggaag ttcttacctc 120
<210> 110
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 110
tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 60
agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 120
<210> 111
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 111
aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 60
tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 120
<210> 112
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 112
caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 60
tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 120
<210> 113
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 113
cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 60
gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 120
<210> 114
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 114
gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 60
ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 120
<210> 115
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 115
agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 60
caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 120
<210> 116
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 116
caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 60
attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 120
<210> 117
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 117
gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 60
ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 120
<210> 118
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 118
gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 60
ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 120
<210> 119
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 119
ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 60
cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 120
<210> 120
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 120
acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 60
cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 120
<210> 121
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 121
tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 60
ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 120
<210> 122
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 122
attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 60
caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 120
<210> 123
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 123
gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 60
ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 120
<210> 124
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 124
ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 60
ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 120
<210> 125
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 125
tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 60
tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 120
<210> 126
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 126
ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 60
agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 120
<210> 127
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 127
ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 60
gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 120
<210> 128
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 128
caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 60
ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 120
<210> 129
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 129
atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 60
aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 120
<210> 130
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 130
gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 60
ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 120
<210> 131
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 131
cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 60
tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 120
<210> 132
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 132
tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 60
taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 120
<210> 133
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 133
aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 60
cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 120
<210> 134
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 134
attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 60
taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 120
<210> 135
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 135
cactaaagga aagagattgg cgagtcaggg aatcttcccc aatgccaaat cactccagcc 60
caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 120
<210> 136
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 136
accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 60
gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 120
<210> 137
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 137
cctgcctcgg cctcccaaag tgctgggatt acaggcatga gccactgcgc ctgaccaact 60
tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 120
<210> 138
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 138
ggtgaatact gttcgagagg ttggctgagg tccaagtatt cctgaaagaa gggaagagag 60
acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 120
<210> 139
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 139
gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 60
aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 120
<210> 140
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 140
gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 60
cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 120
<210> 141
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 141
cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 60
tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 120
<210> 142
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 142
aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 60
tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 120
<210> 143
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 143
acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 60
ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 120
<210> 144
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 144
agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 60
cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 120
<210> 145
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 145
ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 60
ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 120
<210> 146
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 146
ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 60
ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 120
<210> 147
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 147
tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 60
acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 120
<210> 148
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 148
gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 60
tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 120
<210> 149
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 149
tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 60
gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 120
<210> 150
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 150
cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 60
atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 120
<210> 151
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 151
ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 60
aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 120
<210> 152
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 152
agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 60
tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 120
<210> 153
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 153
gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 60
agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 120
<210> 154
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 154
taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 60
accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 120
<210> 155
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 155
gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 60
cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 120
<210> 156
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 156
agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 60
acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 120
<210> 157
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 157
aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 60
attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 120
<210> 158
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 158
tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 60
ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 120
<210> 159
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 159
taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 60
tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 120
<210> 160
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 160
ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 60
atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 120
<210> 161
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 161
tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 60
aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 120
<210> 162
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 162
ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 60
cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 120
<210> 163
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 163
tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 60
agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 120
<210> 164
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 164
tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 60
gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 120
<210> 165
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 165
cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 60
aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 120
<210> 166
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 166
cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 60
gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 120
<210> 167
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 167
atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 60
gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 120
<210> 168
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 168
acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 60
gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 120
<210> 169
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 169
gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 60
tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 120
<210> 170
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 170
tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 60
tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 120
<210> 171
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 171
agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 60
cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 120
<210> 172
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 172
gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 60
gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 120
<210> 173
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 173
tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 60
cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 120
<210> 174
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 174
gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 60
acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 120
<210> 175
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 175
ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 60
agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 120
<210> 176
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 176
cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 60
ggcgaagctc cccgaggcgt cttgcgcggc gattaccttg aatggcaacg ctcctccgcg 120
<210> 177
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 177
caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 60
gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 120
<210> 178
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 178
tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 60
acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 120
<210> 179
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 179
ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 60
cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 120
<210> 180
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 180
tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 60
gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 120
<210> 181
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 181
acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 60
ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 120
<210> 182
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 182
aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 60
acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 120
<210> 183
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 183
ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 60
caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 120
<210> 184
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 184
cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 60
taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 120
<210> 185
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 185
tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 60
ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 120
<210> 186
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 186
acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 60
ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 120
<210> 187
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 187
agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 60
cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 120
<210> 188
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 188
ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 60
ttattatttt ctttttgaga tgaagtcttg ctctgtctcc cagactggag tgcagtggcg 120
<210> 189
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 189
cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 60
caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 120
<210> 190
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 190
aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 60
ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 120
<210> 191
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 191
agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 60
aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 120
<210> 192
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 192
ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 60
tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 120
<210> 193
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 193
catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 60
tttttttttt ttttgagacg gagtctcgct ctgtcgccaa ggctggaatg caatggcatg 120
<210> 194
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 194
cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 60
gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 120
<210> 195
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 195
ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 60
tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 120
<210> 196
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 196
aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 60
aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 120
<210> 197
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 197
ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 60
gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 120
<210> 198
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 198
cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 60
acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 120
<210> 199
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 199
attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 60
cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 120
<210> 200
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 200
cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 60
tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 120
<210> 201
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 201
tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 60
tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 120
<210> 202
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 202
cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 60
ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 120
<210> 203
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 203
cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 60
ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 120
<210> 204
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 204
acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 60
gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 120
<210> 205
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 205
ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 60
cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 120
<210> 206
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 206
cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 60
ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 120
<210> 207
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 207
tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 60
agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 120
<210> 208
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 208
acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 60
tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 120
<210> 209
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 209
atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 60
gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 120
<210> 210
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 210
tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 60
aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 120
<210> 211
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 211
tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 60
agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 120
<210> 212
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 212
ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 60
catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 120
<210> 213
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 213
tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 60
tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 120
<210> 214
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 214
taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 60
attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 120
<210> 215
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 215
ccctcctcgg cttcccaaaa tgctgggatt acaggcgaga gcataaatat tttagggaaa 60
ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 120
<210> 216
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 216
gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 60
cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 120
<210> 217
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 217
ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 60
atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 120
<210> 218
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 218
ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 60
tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 120
<210> 219
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 219
tcaccactca ctcacaactt cagtctgaag taccatttgt aacacagcaa aaccgtgtcc 60
ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 120
<210> 220
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 220
caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 60
tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 120
<210> 221
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 221
aagtacagaa caagtggact tcaaaagtcc aaagcatttg attaaaaaaa aaaaaaaaaa 60
aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 120
<210> 222
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 222
cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 60
ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 120
<210> 223
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 223
gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 60
gcccagggag cgaagggaga tgttaagtga gatttattac ctttcccttt tagttttttt 120
<210> 224
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 224
aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 60
ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 120
<210> 225
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 225
ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 60
acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 120
<210> 226
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 226
gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 60
atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 120
<210> 227
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 227
tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 60
ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 120
<210> 228
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 228
ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 60
aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 120
<210> 229
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 229
acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 60
tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 120
<210> 230
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 230
tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 60
aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 120
<210> 231
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 231
ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 60
cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 120
<210> 232
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 232
tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 60
gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 120
<210> 233
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 233
ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 60
cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 120
<210> 234
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 234
ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 60
attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 120
<210> 235
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 235
gtctagaaga gacaacggaa gcaaaatgga caagcacagg acatgagacc tctaagagac 60
gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 120
<210> 236
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 236
attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 60
ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 120
<210> 237
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 237
catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 60
tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 120
<210> 238
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 238
cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 60
ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 120
<210> 239
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 239
cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 60
gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 120
<210> 240
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 240
tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 60
ggacataagg caggttgtct gggccaatct tgctcccatt cacctcgatg tgctttagcc 120
<210> 241
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 241
ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 60
ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 120
<210> 242
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 242
gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 60
tgttatctgt ttctttctcc tctgaagagg agtcatcatc atcatcatca tcctccgagg 120
<210> 243
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 243
gggtggagag gagcagctgg tcaggctcag gagcagggcc caggccagga cctggaggtg 60
tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 120
<210> 244
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 244
tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 60
tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 120
<210> 245
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 245
gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 60
agggatgctc ttggccagct tgtgcacagc catctggctg tggaagtcac tcttcttggt 120
<210> 246
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 246
attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 60
tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 120
<210> 247
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 247
tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 60
ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 120
<210> 248
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 248
atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 60
ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 120
<210> 249
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 249
tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 60
gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 120
<210> 250
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 250
tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 60
gcacacacct gtgccatctg taccatttcc tcccgtgggg aagggtgggc acacagggga 120
<210> 251
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 251
ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 60
tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 120
<210> 252
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 252
gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 60
cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 120
<210> 253
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 253
ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 60
ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 120
<210> 254
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 254
aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 60
aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 120
<210> 255
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 255
gttctcttct ctaccctgca tctctgcact tctaagcagc ccttggggac agttcaaatt 60
catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 120
<210> 256
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 256
caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 60
gcatacacag ccagcagatg cgagtatgca agggaatgcc taaccccttc tctctctttt 120
<210> 257
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 257
gatgcactgg agtcagcaga cacctgcaag gaagagtggg gtcaccctag agcaaggagg 60
ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 120
<210> 258
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 258
ccgggcccct cctccctgct cagggaggtg cgtgcacgca gtggggacgg cctgagctct 60
ggctctggca cgggcagcct tacctggttg gaggtcaagg ccacgatgcg gtccaggtct 120
<210> 259
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 259
ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 60
caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 120
<210> 260
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 260
tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 60
attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 120
<210> 261
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 261
aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 60
caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 120
<210> 262
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 262
tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 60
aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 120
<210> 263
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 263
aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 60
tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 120
<210> 264
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 264
ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 60
tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 120
<210> 265
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 265
gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 60
gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 120
<210> 266
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 266
aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 60
gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 120
<210> 267
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 267
aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 60
taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 120
<210> 268
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 268
gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 60
aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 120
<210> 269
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 269
gcctatgccc ctccccctgc cgtccccggc catcctgccc cccagagtgc tgaggtgtgg 60
ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 120
<210> 270
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 270
taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 60
ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 120
<210> 271
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 271
gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 60
tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 120
<210> 272
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 272
tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 60
ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 120
<210> 273
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 273
gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 60
tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 120
<210> 274
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 274
agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 60
gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 120
<210> 275
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 275
gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 60
gcagtactcc ccgggtggcc aggacacccc cagctccagc tcctcagggg acgactccgt 120
<210> 276
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 276
catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 60
ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 120
<210> 277
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 277
ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 60
tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 120
<210> 278
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 278
tgaggggtcc ctagcagccc accctgctgc tggtgcacag ccactccccg gcatgagact 60
cagtgcagat ggagagacag ctacacagag ctttggtctg tgtgtgtgtg tgtgcgtgtg 120
<210> 279
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 279
atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 60
gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 120
<210> 280
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 280
aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 60
atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 120
<210> 281
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 281
tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 60
gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 120
<210> 282
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 282
ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 60
catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 120
<210> 283
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 283
agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 60
aataaagaca cctggttgct aacctggccc tgtgctttct gtctccagtt ctgggatagg 120
<210> 284
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 284
gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 60
acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 120
<210> 285
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 285
tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 60
aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 120
<210> 286
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 286
acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 60
gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 120
<210> 287
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 287
gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 60
ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 120
<210> 288
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 288
tgtgtgtgtg tgtgcacatc cgcgtgtgcc tgtgtgcgtg cgcatcttgc ctccaggtgc 60
agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 120
<210> 289
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 289
ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 60
gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 120
<210> 290
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 290
ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 60
accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 120
<210> 291
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 291
accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 60
gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 120
<210> 292
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 292
ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 60
gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 120
<210> 293
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 293
catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 60
catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 120
<210> 294
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 294
gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 60
agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 120
<210> 295
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 295
caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 60
cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 120
<210> 296
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 296
tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 60
aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 120
<210> 297
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 297
gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 60
ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 120
<210> 298
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 298
agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 60
tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 120
<210> 299
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 299
agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 60
agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 120
<210> 300
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 300
ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 60
ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 120
<210> 301
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 301
atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 60
atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 120
<210> 302
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 302
tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 60
aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 120
<210> 303
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 303
ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 60
caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 120
<210> 304
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 304
cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 60
aaggggcttc tagaaggaag ttcttacctc attggttgtg agagtgagaa ttcgatccaa 120
<210> 305
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 305
caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 60
ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 120
<210> 306
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 306
atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 60
cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 120
<210> 307
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 307
caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 60
gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 120
<210> 308
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 308
actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 60
cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 120
<210> 309
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 309
cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 60
cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 120
<210> 310
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 310
agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 60
gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 120
<210> 311
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 311
aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 60
ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 120
<210> 312
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 312
ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 60
gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 120
<210> 313
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 313
tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 60
taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 120
<210> 314
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 314
aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 60
ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 120
<210> 315
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 315
gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 60
tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 120
<210> 316
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 316
atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 60
gtttattttg ttggcaagga tcataaatta ttattatttt ctttttgaga tgaagtcttg 120
<210> 317
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 317
tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 60
aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 120
<210> 318
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 318
gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 60
aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 120
<210> 319
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 319
ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 60
acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 120
<210> 320
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 320
ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 60
ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 120
<210> 321
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 321
cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 60
ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 120
<210> 322
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 322
gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 60
ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 120
<210> 323
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 323
agttgccgac ccctgttctg aagctatgaa aagctttcta aattgggaac ttgtgggtct 60
ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 120
<210> 324
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 324
cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 60
gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 120
<210> 325
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 325
gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 60
aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 120
<210> 326
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 326
agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 60
gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 120
<210> 327
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 327
ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 60
actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 120
<210> 328
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 328
ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 60
ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 120
<210> 329
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 329
aatcttcccc aatgccaaat cactccagcc caagtgggat aaaaccattt ttcagcctct 60
caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 120
<210> 330
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 330
atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 60
agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 120
<210> 331
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 331
acaggcatga gccactgcgc ctgaccaact tttcccagtt tctcaatgaa gccataaact 60
ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 120
<210> 332
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 332
tccaagtatt cctgaaagaa gggaagagag acgttttatt tcatcttggg tcaggataac 60
aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 120
<210> 333
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 333
tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 60
ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 120
<210> 334
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 334
tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 60
gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 120
<210> 335
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 335
caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 60
gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 120
<210> 336
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 336
agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 60
agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 120
<210> 337
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 337
cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 60
cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 120
<210> 338
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 338
ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 60
caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 120
<210> 339
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 339
gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 60
ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 120
<210> 340
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 340
gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 60
gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 120
<210> 341
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 341
tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 60
tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 120
<210> 342
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 342
tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 60
catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 120
<210> 343
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 343
atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 60
agaacacatc tatttacata ttgtctgtgg ctactttgag gctacagtgg cgaagttgca 120
<210> 344
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 344
gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 60
tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 120
<210> 345
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 345
tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 60
aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 120
<210> 346
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 346
ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 60
tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 120
<210> 347
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 347
aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 60
agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 120
<210> 348
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 348
agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 60
atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 120
<210> 349
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 349
ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 60
caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 120
<210> 350
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 350
aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 60
cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 120
<210> 351
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 351
ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 60
ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 120
<210> 352
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 352
cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 60
aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 120
<210> 353
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 353
ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 60
ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 120
<210> 354
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 354
aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 60
caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 120
<210> 355
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 355
gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 60
cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 120
<210> 356
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 356
catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 60
cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 120
<210> 357
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 357
tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 60
tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 120
<210> 358
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 358
ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 60
atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 120
<210> 359
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 359
ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 60
ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 120
<210> 360
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 360
tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 60
aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 120
<210> 361
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 361
agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 60
cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 120
<210> 362
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 362
ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 60
tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 120
<210> 363
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 363
aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 60
tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 120
<210> 364
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 364
accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 60
ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 120
<210> 365
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 365
cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 60
tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 120
<210> 366
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 366
tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 60
acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 120
<210> 367
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 367
ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 60
gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 120
<210> 368
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 368
tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 60
aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 120
<210> 369
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 369
cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 60
gattaccttg aatggcaacg ctcctccgcg acctgtgttg tccccgcgcc ccgcgtgggt 120
<210> 370
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 370
ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 60
ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 120
<210> 371
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 371
gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 60
agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 120
<210> 372
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 372
taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 60
ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 120
<210> 373
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 373
atggtgtgtg cgtgcacggt gtgtgcatgt gctttcagat agcttgaaag atttttcctc 60
ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 120
<210> 374
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 374
tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 60
cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 120
<210> 375
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 375
aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 60
agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 120
<210> 376
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 376
cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 60
caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 120
<210> 377
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 377
aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 60
agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 120
<210> 378
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 378
atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 60
gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 120
<210> 379
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 379
aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 60
aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 120
<210> 380
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 380
cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 60
ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 120
<210> 381
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 381
gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 60
ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 120
<210> 382
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 382
caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 60
ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 120
<210> 383
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 383
gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 60
agcacaggtg aaacggtgtg attcacagca aaagcaagaa aagtatgtgt gcagtgtgtg 120
<210> 384
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 384
agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 60
aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 120
<210> 385
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 385
ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 60
tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 120
<210> 386
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 386
ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 60
atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 120
<210> 387
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 387
aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 60
gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 120
<210> 388
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 388
ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 60
tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 120
<210> 389
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 389
ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 60
gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 120
<210> 390
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 390
tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 60
tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 120
<210> 391
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 391
ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 60
atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 120
<210> 392
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 392
ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 60
tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 120
<210> 393
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 393
caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 60
gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 120
<210> 394
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 394
tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 60
tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 120
<210> 395
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 395
ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 60
ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 120
<210> 396
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 396
acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 60
agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 120
<210> 397
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 397
acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 60
accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 120
<210> 398
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 398
agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 60
ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 120
<210> 399
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 399
cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 60
cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 120
<210> 400
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 400
aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 60
agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 120
<210> 401
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 401
ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 60
gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 120
<210> 402
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 402
aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 60
ttttcccggt ttctttcttt tttattttta tttttttttt ttttgagacg gagtctcgct 120
<210> 403
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 403
gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 60
atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 120
<210> 404
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 404
gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 60
tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 120
<210> 405
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 405
acaggcgaga gcataaatat tttagggaaa ataaatattt taacaaaagg tacatgtttt 60
tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 120
<210> 406
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 406
gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 60
gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 120
<210> 407
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 407
cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 60
cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 120
<210> 408
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 408
gatgctccag gtggcataac ggacctgagg ggaaatgcca aagggataca ttgagggtcc 60
agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 120
<210> 409
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 409
agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 60
gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 120
<210> 410
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 410
gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 60
ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 120
<210> 411
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 411
ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 60
tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 120
<210> 412
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 412
tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 60
caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 120
<210> 413
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 413
caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 60
ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 120
<210> 414
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 414
ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 60
ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 120
<210> 415
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 415
ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 60
agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 120
<210> 416
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 416
agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 60
tcttgcagca tttaaagcat ggtgtcttgc atgtagtgag tgcatggtga attaatttaa 120
<210> 417
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 417
tgcttacacc ctttaagtta gtaatttcac ttgtaacaat ctgtcctaag gaagcacgct 60
taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 120
<210> 418
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 418
taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 60
tacctaatat cacaaaatag ttcattaaat tataggactt tttctgttgt tttttggaga 120
<210> 419
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 419
tggaatctta tacagtcatg aaaaattatg tttcaaatat tcatgcttga aatatatcag 60
gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 120
<210> 420
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 420
gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 60
atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 120
<210> 421
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 421
atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 60
tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 120
<210> 422
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 422
tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 60
gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 120
<210> 423
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 423
gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 60
cgcatgcaca cacacgtacc ttgtagcctc caattctgtg gtcaggtttg aattctttgc 120
<210> 424
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 424
agggctagaa gcgcgacggc ggtagcagct aggcttggcc cccggcgtgg agcagacgcg 60
gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 120
<210> 425
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 425
gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 60
ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 120
<210> 426
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 426
ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 60
gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 120
<210> 427
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 427
gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 60
tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 120
<210> 428
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 428
tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 60
cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 120
<210> 429
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 429
cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 60
gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 120
<210> 430
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 430
gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 60
tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 120
<210> 431
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 431
tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 60
ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 120
<210> 432
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 432
ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 60
acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 120
<210> 433
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 433
acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 60
gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 120
<210> 434
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 434
gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 60
gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 120
<210> 435
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 435
gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 60
ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 120
<210> 436
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 436
ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 60
tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 120
<210> 437
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 437
tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 60
gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 120
<210> 438
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 438
gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 60
ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 120
<210> 439
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 439
ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 60
ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 120
<210> 440
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 440
ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 60
ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 120
<210> 441
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 441
ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 60
tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 120
<210> 442
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 442
tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 60
ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 120
<210> 443
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 443
ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 60
gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 120
<210> 444
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 444
gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 60
ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 120
<210> 445
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 445
ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 60
ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 120
<210> 446
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 446
ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 60
gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 120
<210> 447
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 447
gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 60
cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 120
<210> 448
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 448
cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 60
ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 120
<210> 449
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 449
ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 60
ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 120
<210> 450
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 450
ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 60
catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 120
<210> 451
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 451
catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 60
tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 120
<210> 452
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 452
tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 60
tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 120
<210> 453
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 453
tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 60
ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 120
<210> 454
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 454
ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 60
tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 120
<210> 455
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 455
tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 60
gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 120
<210> 456
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 456
gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 60
gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 120
<210> 457
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 457
gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 60
aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 120
<210> 458
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 458
aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 60
cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 120
<210> 459
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 459
cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 60
agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 120
<210> 460
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 460
agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 60
cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 120
<210> 461
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 461
cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 60
gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 120
<210> 462
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 462
gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 60
aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 120
<210> 463
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 463
aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 60
gttcttgcag gaaagtgctg tcttgttcta tcaggccttg gctggatttt ttgtttgttt 120
<210> 464
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 464
aactgcaagt ggatggcgag gcggtgtctg tctttgccta gaaagggagg atcctggtgg 60
cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 120
<210> 465
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 465
cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 60
tccagggtgg gaagggaccc cagaggcatg attttctgtt ggttttttgc tgatgtaact 120
<210> 466
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 466
tttaagcaga ttttagatgt gcagagaaaa gcataaggaa cagaatacca ctgatgcttc 60
tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 120
<210> 467
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 467
tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 60
gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 120
<210> 468
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 468
gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 60
tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 120
<210> 469
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 469
tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 60
aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 120
<210> 470
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 470
aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 60
agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 120
<210> 471
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 471
agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 60
ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 120
<210> 472
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 472
ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 60
aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 120
<210> 473
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 473
aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 60
ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 120
<210> 474
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 474
ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 60
ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 120
<210> 475
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 475
ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 60
tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 120
<210> 476
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 476
tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 60
gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 120
<210> 477
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 477
gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 60
acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 120
<210> 478
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 478
acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 60
actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 120
<210> 479
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 479
actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 60
gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 120
<210> 480
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 480
gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 60
ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 120
<210> 481
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 481
ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 60
ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 120
<210> 482
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 482
ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 60
ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 120
<210> 483
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 483
ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 60
ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 120
<210> 484
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 484
ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 60
aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 120
<210> 485
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 485
aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 60
gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 120
<210> 486
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 486
gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 60
aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 120
<210> 487
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 487
aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 60
agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 120
<210> 488
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 488
agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 60
acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 120
<210> 489
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 489
acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 60
cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 120
<210> 490
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 490
cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 60
cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 120
<210> 491
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 491
cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 60
tggtgggcag gtgctcgaat ggatttttta caggaaatgg tggatgccgg gtgcggtggc 120
<210> 492
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 492
gcatcagctt gaggcatttg ggtgtgctgt cccacatctg accccccagg cagcagcctg 60
tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 120
<210> 493
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 493
tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 60
ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 120
<210> 494
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 494
ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 60
aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 120
<210> 495
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 495
aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 60
ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 120
<210> 496
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 496
ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 60
tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 120
<210> 497
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 497
tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 60
gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 120
<210> 498
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 498
gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 60
aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 120
<210> 499
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 499
aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 60
aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 120
<210> 500
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 500
aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 60
ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 120
<210> 501
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 501
ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 60
tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 120
<210> 502
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 502
tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 60
ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 120
<210> 503
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 503
ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 60
gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 120
<210> 504
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 504
gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 60
accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 120
<210> 505
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 505
accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 60
gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 120
<210> 506
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 506
gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 60
gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 120
<210> 507
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 507
gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 60
ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 120
<210> 508
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 508
ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 60
acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 120
<210> 509
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 509
acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 60
agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 120
<210> 510
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 510
agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 60
gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 120
<210> 511
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 511
gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 60
gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 120
<210> 512
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 512
gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 60
tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 120
<210> 513
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 513
tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 60
gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 120
<210> 514
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 514
gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 60
ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 120
<210> 515
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 515
ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 60
gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 120
<210> 516
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 516
gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 60
cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 120
<210> 517
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 517
cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 60
agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 120
<210> 518
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 518
agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 60
tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 120
<210> 519
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 519
tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 60
ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 120
<210> 520
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 520
ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 60
ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 120
<210> 521
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 521
ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 60
taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 120
<210> 522
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 522
taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 60
agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 120
<210> 523
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 523
agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 60
cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 120
<210> 524
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 524
cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 60
tcctggctga atggtcttag gctacatctc ctagagttgc tcagtgggag attgtcctgc 120
<210> 525
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 525
tgggtggctt gctgcccaca cctgccccag cctccacatt tgaagccttt tgtcagtttc 60
acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 120
<210> 526
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 526
acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 60
agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 120
<210> 527
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 527
agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 60
ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 120
<210> 528
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 528
ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 60
agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 120
<210> 529
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 529
agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 60
ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 120
<210> 530
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 530
ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 60
ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 120
<210> 531
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 531
ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 60
ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 120
<210> 532
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 532
ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 60
tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 120
<210> 533
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 533
tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 60
gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 120
<210> 534
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 534
gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 60
ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 120
<210> 535
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 535
ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 60
gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 120
<210> 536
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 536
gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 60
tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 120
<210> 537
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 537
tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 60
tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 120
<210> 538
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 538
tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 60
ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 120
<210> 539
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 539
ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 60
gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 120
<210> 540
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 540
gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 60
gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 120
<210> 541
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 541
gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 60
atgagcagga gaggtgaaca atgcagtgcg ctaatgagat acaagtgtag agtcaacaac 120
<210> 542
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 542
tgcagttaaa taaatacggt ttgtccgtaa gattggaggc tgcagttaaa taaatacggt 60
ttgtccgtaa gattggaggc tgcagttaaa taaatacggt ttgtccgtaa gattggaggc 120
<210> 543
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 543
aataaatacg gtttgtccgt aagattggaa ggtggaacgg tccttaaatt aaagtgcatt 60
tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 120
<210> 544
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 544
tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 60
caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 120
<210> 545
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 545
caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 60
gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 120
<210> 546
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 546
gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 60
tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 120
<210> 547
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 547
tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 60
cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 120
<210> 548
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 548
cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 60
cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 120
<210> 549
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 549
cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 60
ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 120
<210> 550
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 550
ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 60
atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 120
<210> 551
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 551
atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 60
tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 120
<210> 552
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 552
tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 60
actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 120
<210> 553
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 553
actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 60
ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 120
<210> 554
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 554
ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 60
ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 120
<210> 555
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 555
ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 60
aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 120
<210> 556
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 556
aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 60
gaggtaaatc ccttttattc aagaagtgag ggaaaggaaa aaatacaaag ttaagtccct 120
<210> 557
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 557
gacaaggcca tagcagagaa ggacctacaa gtttttgcta gactaaataa ttggcaaccg 60
ttttttagct taattgagaa aaggagagaa tgagcaaata ctgctaggca tgaagaaagg 120
<210> 558
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 558
ccacccttag ttttaatcaa aagttatatt atcaaaaatt aaaagacttg tctagggtaa 60
ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 120
<210> 559
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 559
ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 60
gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 120
<210> 560
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 560
gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 60
cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 120
<210> 561
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 561
cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 60
acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 120
<210> 562
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 562
acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 60
gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 120
<210> 563
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 563
gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 60
ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 120
<210> 564
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 564
ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 60
tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 120
<210> 565
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 565
tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 60
gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 120
<210> 566
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 566
gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 60
aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 120
<210> 567
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 567
aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 60
cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 120
<210> 568
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 568
cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 60
gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 120
<210> 569
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 569
gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 60
gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 120
<210> 570
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 570
gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 60
tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 120
<210> 571
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 571
tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 60
gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 120
<210> 572
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 572
gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 60
agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 120
<210> 573
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 573
agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 60
gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 120
<210> 574
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 574
gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 60
tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 120
<210> 575
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 575
tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 60
gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 120
<210> 576
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 576
gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 60
cagggctgct gccttgctgg ttacagccac cgttctaggt atattaaaga tctaaactta 120
<210> 577
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 577
gcaaactatc acctgtgggc cagacttggc cactgcctgt ttgtgtaaag ttttatcgtc 60
acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 120
<210> 578
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 578
acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 60
gagtagtgtg acagaggctg tgtggccttc aaatcctaac acatggccgg gcgcagtggc 120
<210> 579
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 579
aacagccccc tgatgaggca gcactggctc cgctcagcct gcagtgggta atgaagcgtc 60
accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 120
<210> 580
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 580
accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 60
cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 120
<210> 581
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 581
cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 60
acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 120
<210> 582
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 582
acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 60
gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 120
<210> 583
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 583
gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 60
tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 120
<210> 584
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 584
tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 60
atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 120
<210> 585
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 585
atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 60
tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 120
<210> 586
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 586
tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 60
cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 120
<210> 587
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 587
cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 60
tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 120
<210> 588
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 588
tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 60
cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 120
<210> 589
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 589
cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 60
aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 120
<210> 590
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 590
aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 60
ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 120
<210> 591
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 591
ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 60
tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 120
<210> 592
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 592
tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 60
gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 120
<210> 593
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 593
gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 60
tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 120
<210> 594
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 594
tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 60
gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 120
<210> 595
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 595
gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 60
ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 120
<210> 596
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 596
ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 60
gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 120
<210> 597
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 597
gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 60
catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 120
<210> 598
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 598
catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 60
catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 120
<210> 599
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 599
catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 60
cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 120
<210> 600
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 600
cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 60
cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 120
<210> 601
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 601
cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 60
acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 120
<210> 602
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 602
acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 60
gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 120
<210> 603
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 603
gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 60
ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 120
<210> 604
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 604
ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 60
gggggcaggt ggcccctgca tgtaacttat gttagaaatg cacaatgctt gttctctggt 120
<210> 605
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 605
ctgccactgg gctgcggggg cagctgcagg gacacacacc tgttgggtgc ctgctggttc 60
cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 120
<210> 606
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 606
cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 60
gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 120
<210> 607
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 607
gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 60
ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 120
<210> 608
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 608
ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 60
aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 120
<210> 609
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 609
aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 60
taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 120
<210> 610
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 610
taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 60
agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 120
<210> 611
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 611
agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 60
gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 120
<210> 612
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 612
gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 60
cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 120
<210> 613
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 613
cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 60
tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 120
<210> 614
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 614
tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 60
gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 120
<210> 615
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 615
gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 60
ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 120
<210> 616
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 616
ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 60
gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 120
<210> 617
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 617
gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 60
ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 120
<210> 618
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 618
ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 60
tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 120
<210> 619
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 619
tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 60
ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 120
<210> 620
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 620
ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 60
gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 120
<210> 621
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 621
gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 60
tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 120
<210> 622
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 622
tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 60
ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 120
<210> 623
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 623
ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 60
gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 120
<210> 624
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223> Probe
<400> 624
gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 60
ccagttccct gcggatctga tgtggaggaa acagctgttt cctgggcttt gcatccggcc 120
Claims (9)
1. The probe combination for detecting the gene fusion of the human gene FGFR1/2/3 comprises a probe with a probe sequence of SEQ ID No. 1-624.
2. The probe combination of claim 1, wherein the human gene FGFR1/2/3 gene fusion comprises FGFR2-COL14A1, FGFR3-TACC3, and FGFR3-BAIAP2L1.
3. Use of a combination of probes according to claim 1 or 2 for the preparation of a kit for the detection of the human gene FGFR1/2/3 gene fusion in a sample from a subject.
4. The use of claim 3, wherein the sample comprises a tissue biopsy sample, an FFPE sample, and a cfDNA sample, and the subject is a normal healthy subject, a subject suspected or diagnosed as having a disease.
5. The use of claim 3, wherein the sample comprises a DNA sample and liquid biopsy plasma.
6. The use of claim 4, wherein the disease is cancer.
7. A kit for detecting a human gene FGFR1/2/3 gene fusion comprising a capture reagent comprising the probe combination of claim 1 or 2.
8. The kit of claim 7, further comprising one or more of: 1) a library building reagent, 2) a capture reagent other than a probe, 3) a solid support, and/or 4) a high throughput sequencing reagent.
9. The kit of claim 8, wherein the pooling reagents comprise a DNA polymerase, a ligase, a buffer and/or a quality control; the capture reagent other than the probe comprises a blocking agent, a capture buffer and/or a washing solution; the solid phase carrier comprises magnetic beads.
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| CN113249444B (en) * | 2021-05-17 | 2023-10-13 | 苏州吉因加生物医学工程有限公司 | DNA probe library for hybridization with FGFR1, 2 or 3 genes and application thereof |
| CN113249490B (en) * | 2021-07-06 | 2021-10-12 | 迈杰转化医学研究(苏州)有限公司 | Probe library for detecting FGFR family gene inhibitor sensitivity and drug resistance variation and application thereof |
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| WO2014144121A2 (en) * | 2013-03-15 | 2014-09-18 | Life Technologies Corporation | Classification and actionability indices for lung cancer |
| CN106480205A (en) * | 2016-11-11 | 2017-03-08 | 北京吉因加科技有限公司 | For detecting combined sequence and the probe of various mutations type simultaneously |
| CN109609647A (en) * | 2019-01-25 | 2019-04-12 | 臻悦生物科技江苏有限公司 | Detection Panel, detection kit and its application for the targeting of general cancer kind, chemotherapy and immune medication based on the sequencing of two generations |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2014144121A2 (en) * | 2013-03-15 | 2014-09-18 | Life Technologies Corporation | Classification and actionability indices for lung cancer |
| CN106480205A (en) * | 2016-11-11 | 2017-03-08 | 北京吉因加科技有限公司 | For detecting combined sequence and the probe of various mutations type simultaneously |
| CN109609647A (en) * | 2019-01-25 | 2019-04-12 | 臻悦生物科技江苏有限公司 | Detection Panel, detection kit and its application for the targeting of general cancer kind, chemotherapy and immune medication based on the sequencing of two generations |
Non-Patent Citations (1)
| Title |
|---|
| "Identification of Targetable FGFR Gene Fusions in Diverse Cancers";Yi-Mi Wu et al.;《CANCER DISCOVERY》;20130404;第636-647页 * |
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