CN110218797A - FGFR Gene Fusion probe, detection method and kit - Google Patents
FGFR Gene Fusion probe, detection method and kit Download PDFInfo
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- CN110218797A CN110218797A CN201910680223.XA CN201910680223A CN110218797A CN 110218797 A CN110218797 A CN 110218797A CN 201910680223 A CN201910680223 A CN 201910680223A CN 110218797 A CN110218797 A CN 110218797A
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Abstract
The present invention relates to FGFR Gene Fusion probe, detection method and kits.The present invention provides the probe combinations of detection people FGFR Gene Fusion comprising following probes: FGFR2(NM_001144917): whole exons and 1,15, No. 17 probe coverings for including subregion;FGFR3(NM_000142): whole exons and the covering of No. 17,18 intrones and the probe of 3 ' UTR regions;FGFR3 chaperone TACC3(NM_006342) the 11st, No. 14 probe covering for including subregion.The present invention also mentions the kit of detection FGFR Gene Fusion, especially the disposable detection kit of FGFR Gene Fusion and a kind of for analyzing the automation equipment of human gene FGFR Gene Fusion.The present invention is suitable for the detection of extensive sample.
Description
Technical field
The invention belongs to biology technique of gene detection and technical field of biological information, and in particular to Gene Fusion detection
Capture probe design, high-flux sequence (NGS) experiment and data analysing method and kit.
Background technique
Fibroblast growth factor acceptor (FGFR) family mainly include 4 seed types receptor FGFR1, FGFR2,
FGFR3 and FGFR4 plays a significant role in angiogenesis, angiogenesis, wound healing and embryonic development.FGFR is cross-film
Albumen has extracellular domain, tyrosine kinase domain in transmembrane domain and cytoplasm.FGF ligand is in conjunction with FGFR
Signal transduction cascade of events in active cell.
FGFR gene mutation includes that Gene Fusion causes various diseases.Gene Fusion belongs to one kind of genetic mutation, refers to
Since the reasons such as chromosomal structural aberration, including missing, repetition, inversion, transposition lead to different positions on two or more chromosomes
The gene set joins end to end the mosaic gene of composition, and expression product is fusion protein.In Gene Fusion, the whole of two genes
Or the sequence of a part mutually permeates a new gene.Gene Fusion is likely to be chromosome translocation, intercalary delection or dye
Colour solid is inverted caused result.The driving gene that fusion is cancer occurs for certain kinases, and corresponding gene target inhibitor is also
Through granted, including the inhibitor Gleevec for BCR-ABL fusion;Buddhist nun is replaced for the inhibitor gram azoles of ALK fusion
(crizotinib), Ai Le replaces Buddhist nun (alectinib), Ceritinib (ceritinib), and Laura replaces Buddhist nun (lorlatinib);Needle
Buddhist nun (crizotinib) is replaced to the inhibitor gram azoles of ROS1 fusion;For the inhibitor Larotrectinib of NTRK fusion.Tradition
The method for detecting these Gene Fusions is usually FISH and IHC, but these flux are lower, and needs individually to set for individual gene
Kit is counted, and the chaperone merged is generally difficult to detect, therefore also restricted in clinical practice.
High throughput sequencing technologies are also known as next-generation sequencing technologies (Next Generation Sequencing, abbreviation
NGS), once sequencing can be carried out to the hundreds of thousands in cellular genome to up to ten million molecules parallel, therefore in genome
It is widely used in research.A large amount of sequences can be quickly sequenced in high-flux sequence, can be used in Different Individual
Such as the detection in Gene Mutation of tumor patient, provide for individualized treatment beneficial to hints and tips.NGS provides a kind of detection fusion
High throughput, the method for high-precision and hypersensitivity.
Summary of the invention
The present invention provides a kind of highly sensitive and is specifically used for detection FGFR1, FGFR2 and FGFR3 Gene Fusion (especially
Human gene FGFR2/3 Gene Fusion) variation capture probe, kit and data analysing method.Detection of the invention
Kit can be used for various samples, such as tumor tissue biopsy sample, FFPE sample and liquid biopsy blood plasma, and cfDNA etc. is no
The DNA sample of same type.Method of the invention and the sensibility and specificity of kit detection are high, reproducible, press down for FGFR
The clinical research of preparation provides a kind of biomarker screening method.
In some embodiments, the present invention is provided to detect human gene FGFR1/2/3 Gene Fusion (especially people
Genoid FGFR2/3 Gene Fusion) probe combinations comprising FGFR2-COL14A1, FGFR3-TACC3, FGFR3-
BAIAP2L1 merges the probe of position, and the probe combinations include following probes selected from subordinate list one:
For the multicore glycosides of initiation site 1729490 on No. 4 chromosomes where people's TACC3 gene to termination site 1729609
Acid sequence or its complementary series, probe sequence are SEQ ID No:501,
For the multicore glycosides of initiation site 1729550 on No. 4 chromosomes where people's TACC3 gene to termination site 1729669
Acid sequence or its complementary series, probe sequence are SEQ ID No:502,
For the multicore glycosides of initiation site 1729610 on No. 4 chromosomes where people's TACC3 gene to termination site 1729729
Acid sequence or its complementary series, probe sequence are SEQ ID No:503,
For the multicore glycosides of initiation site 1729670 on No. 4 chromosomes where people's TACC3 gene to termination site 1729789
Acid sequence or its complementary series, probe sequence are SEQ ID No:504,
For the multicore glycosides of initiation site 1729730 on No. 4 chromosomes where people's TACC3 gene to termination site 1729849
Acid sequence or its complementary series, probe sequence are SEQ ID No:505,
For the multicore glycosides of initiation site 1729790 on No. 4 chromosomes where people's TACC3 gene to termination site 1729909
Acid sequence or its complementary series, probe sequence are SEQ ID No:506,
For the multicore glycosides of initiation site 1808662 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808781
Acid sequence or its complementary series, probe sequence are SEQ ID No:72,
For the multicore glycosides of initiation site 1808782 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808901
Acid sequence or its complementary series, probe sequence are SEQ ID No:79,
For the multicore glycosides of initiation site 1808722 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808841
Acid sequence or its complementary series, probe sequence are SEQ ID No:96,
For the multicore glycosides of initiation site 1808692 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808811
Acid sequence or its complementary series, probe sequence are SEQ ID No:269,
For the multicore glycosides of initiation site 1808752 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808871
Acid sequence or its complementary series, probe sequence are SEQ ID No:292,
For the multicore glycosides of initiation site 1808812 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808931
Acid sequence or its complementary series, probe sequence are SEQ ID No:275,
For the more of initiation site 123241115 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241234
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:111,
For the more of initiation site 123241175 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241294
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:158,
For the more of initiation site 123241055 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241174
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:165,
For the more of initiation site 123240995 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241114
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:187,
For the more of initiation site 123241295 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241414
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:193,
For the more of initiation site 123241235 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241354
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:212,
For the more of initiation site 123240965 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241084
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:295,
For the more of initiation site 123241145 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241264
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:306,
For the more of initiation site 123241205 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241324
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:352,
For the more of initiation site 123241085 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241204
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:358,
For the more of initiation site 123241025 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241144
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:380,
For the more of initiation site 123241265 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241384
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:402,
For the more of initiation site 123240935 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241054
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:100,
For the more of initiation site 123242135 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242254
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:106,
For the more of initiation site 123243095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243214
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:109,
For the more of initiation site 123240755 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240874
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:113,
For the more of initiation site 123240815 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240934
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:114,
For the more of initiation site 123240275 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240394
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:115,
For the more of initiation site 123242195 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242314
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:121,
For the more of initiation site 123240515 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240634
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:123,
For the more of initiation site 123241655 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241774
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:125,
For the more of initiation site 123240335 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240454
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:128,
For the more of initiation site 123240635 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240754
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:129,
For the more of initiation site 123239735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239854
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:131,
For the more of initiation site 123240695 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240814
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:133,
For the more of initiation site 123241595 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241714
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:137,
For the more of initiation site 123239495 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239614
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:138,
For the more of initiation site 123239915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240034
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:139,
For the more of initiation site 123242735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242854
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:143,
For the more of initiation site 123242975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243094
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:148,
For the more of initiation site 123240455 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240574
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:149,
For the more of initiation site 123240095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240214
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:150,
For the more of initiation site 123241895 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242014
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:151,
For the more of initiation site 123240215 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240334
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:153,
For the more of initiation site 123242675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242794
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:156,
For the more of initiation site 123239975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240094
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:157,
For the more of initiation site 123240575 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240694
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:160,
For the more of initiation site 123243035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243154
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:163,
For the more of initiation site 123239855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239974
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:164,
For the more of initiation site 123240155 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240274
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:167,
For the more of initiation site 123239795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239914
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:170,
For the more of initiation site 123241775 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241894
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:171,
For the more of initiation site 123242915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243034
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:172,
For the more of initiation site 123242615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242734
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:175,
For the more of initiation site 123241955 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242074
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:182,
For the more of initiation site 123240875 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240994
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:183,
For the more of initiation site 123239555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239674
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:186,
For the more of initiation site 123242255 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242374
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:188,
For the more of initiation site 123240395 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240514
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:195,
For the more of initiation site 123239615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239734
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:197,
For the more of initiation site 123240035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240154
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:199,
For the more of initiation site 123242075 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242194
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:201,
For the more of initiation site 123241835 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241954
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:202,
For the more of initiation site 123241715 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241834
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:207,
For the more of initiation site 123242015 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242134
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:208,
For the more of initiation site 123242855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242974
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:209,
For the more of initiation site 123242555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242674
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:215,
For the more of initiation site 123239675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239794
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:216,
For the more of initiation site 123242795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242914
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:217,
For the more of initiation site 123242165 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242284
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:301,
For the more of initiation site 123243125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243244
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:304,
For the more of initiation site 123240785 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240904
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:308,
For the more of initiation site 123240845 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240964
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:309,
For the more of initiation site 123240305 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240424
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:310,
For the more of initiation site 123242225 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242344
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:316,
For the more of initiation site 123241685 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241804
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:319,
For the more of initiation site 123240365 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240484
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:322,
For the more of initiation site 123240665 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240784
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:323,
For the more of initiation site 123239765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239884
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:325,
For the more of initiation site 123240725 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240844
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:327,
For the more of initiation site 123241625 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241744
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:331,
For the more of initiation site 123239525 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239644
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:332,
For the more of initiation site 123239945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240064
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:333,
For the more of initiation site 123242765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242884
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:337,
For the more of initiation site 123243005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243124
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:342,
For the more of initiation site 123240485 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240604
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:343,
For the more of initiation site 123240125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240244
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:344,
For the more of initiation site 123241925 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242044
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:345,
For the more of initiation site 123240245 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240364
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:347,
For the more of initiation site 123242705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242824
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:350,
For the more of initiation site 123240005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240124
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:351,
For the more of initiation site 123243065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243184
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:356,
For the more of initiation site 123239885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240004
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:357,
For the more of initiation site 123240185 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240304
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:360,
For the more of initiation site 123239825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239944
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:363,
For the more of initiation site 123241805 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241924
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:364,
For the more of initiation site 123242945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243064
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:365,
For the more of initiation site 123242645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242764
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:368,
For the more of initiation site 123241985 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242104
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:375,
For the more of initiation site 123240905 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241024
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:376,
For the more of initiation site 123239585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239704
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:379,
For the more of initiation site 123240425 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240544
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:386,
For the more of initiation site 123239645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239764
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:387,
For the more of initiation site 123240065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240184
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:389,
For the more of initiation site 123242105 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242224
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:391,
For the more of initiation site 123241865 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241984
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:392,
For the more of initiation site 123241745 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241864
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:397,
For the more of initiation site 123242045 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242164
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:398,
For the more of initiation site 123242885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243004
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:399,
For the more of initiation site 123242585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242704
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:405,
For the more of initiation site 123239705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239824
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:406,
For the more of initiation site 123242825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242944
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:407,
For the multicore glycosides of initiation site 1809043 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809162
Acid sequence or its complementary series, probe sequence are SEQ ID No:74,
For the multicore glycosides of initiation site 1809163 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809282
Acid sequence or its complementary series, probe sequence are SEQ ID No:78,
For the multicore glycosides of initiation site 1808983 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809102
Acid sequence or its complementary series, probe sequence are SEQ ID No:82,
For the multicore glycosides of initiation site 1809283 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809402
Acid sequence or its complementary series, probe sequence are SEQ ID No:86,
For the multicore glycosides of initiation site 1809223 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809342
Acid sequence or its complementary series, probe sequence are SEQ ID No:91,
For the multicore glycosides of initiation site 1809103 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809222
Acid sequence or its complementary series, probe sequence are SEQ ID No:92,
For the multicore glycosides of initiation site 1809343 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809462
Acid sequence or its complementary series, probe sequence are SEQ ID No:97,
For the multicore glycosides of initiation site 1809193 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809312
Acid sequence or its complementary series, probe sequence are SEQ ID No:274,
For the multicore glycosides of initiation site 1809013 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809132
Acid sequence or its complementary series, probe sequence are SEQ ID No:278,
For the multicore glycosides of initiation site 1809313 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809432
Acid sequence or its complementary series, probe sequence are SEQ ID No:282,
For the multicore glycosides of initiation site 1809253 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809372
Acid sequence or its complementary series, probe sequence are SEQ ID No:287,
For the multicore glycosides of initiation site 1809133 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809252
Acid sequence or its complementary series, probe sequence are SEQ ID No:288.
In some embodiments, it has been found that probe combinations of the invention can be wide with highly sensitive and specific detection
General FGFR1/2/3(especially FGFR2/3) Gene Fusion, especially include such as FGFR2-COL14A1, FGFR3-TACC3 and
FGFR3-BAIAP2L1 fusion.In some embodiments, it has been found that probe combinations of the invention can with highly sensitive and
The extensive FGFR1/2/3(of specific detection especially FGFR2/3) Gene Fusion, especially include such as ZNF198-FGFR1,
FGFR2-BICC1 and FGFR3-WHSC1.
In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene
The probe combinations of fusion include the probe selected from following one or more fusions for FGFR1 selected from subordinate list one:
BAG4-FGFR1、FGFR1OP-FGFR1、FN1-FGFR1、CEP110-FGFR1、TPR-FGFR1、BCR-FGFR1、CPSF6-
FGFR1、ERLIN2-FGFR1、FGFR1-FGFR1、FGFR1-TACC1、HERV-K-FGFR1、ACPP-FGFR1、CUX1-
FGFR1、FGFR1-ADAM18、FGFR1-ADGRA2、FGFR1-CSMD1、FGFR1-LETM2、FGFR1OP2-FGFR1、FGFR1-
TTLL1、FOXO1-FGFR1、LRRFIP1-FGFR1、MYO18A-FGFR1、NUP98-FGFR1、RAB11FIP1-FGFR1、
RANBP2-FGFR1, RHOT1-FGFR1, SQSTM1-FGFR1, TRIM24-FGFR1 and/or ZNF198-FGFR1.
In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene
The probe combinations of fusion include the probe selected from following one or more fusions for FGFR2 selected from subordinate list one:
FGFR2-BICC1、FGFR2-KIAA1217、FGFR2-AHCYL1、FGFR2-CCDC6、FGFR2-NOL4、FGFR2-TACC2、
FGFR2-KIAA1967、FGFR2-NPM1、FGFR2-NRAP、FGFR2-POC1B、FGFR2-ARHGAP24、FGFR2-CASP7、
FGFR2-CIT、FGFR2-KIAA1598、FGFR2-LAMC1、FGFR2-MACF1、FGFR2-OFD1、FGFR2-PAWR、FGFR2-
SHTN1、FGFR2-SPERT、FGFR2-TACC1、FGFR2-TACC3、FGFR2-WAC、AHCYL1-FGFR2、CCAR2-FGFR2、
CCDC46-FGFR2、CTNNA3-FGFR2、DNM1-FGFR2、FGFR2-ACLY、FGFR2-AFF1、FGFR2-AFF3、FGFR2-
AFR、FGFR2-AP1M1、FGFR2-ATF1、FGFR2-ATF2、FGFR2-BFSP2、FGFR2-C10ORF118、FGFR2-
C10orf68、FGFR2-CBX5、FGFR2-CCAR2、FGFR2-CCDC158、FGFR2-CCDC186、FGFR2-COL16A1、
FGFR2-CTNNA3、FGFR2-EIF4A2、FGFR2-ENPP2、FGFR2-ETV6、FGFR2-FAM76A、FGFR2-FILIP1、
FGFR2-FRK、FGFR2-GAB2、FGFR2-GOPC、FGFR2-KCNH7、FGFR2-KIF14、FGFR2-KLRAQ1、FGFR2-
MGEA5、FGFR2-NCALD、FGFR2-NRBF2、FGFR2-PPAPDC1A、FGFR2-PPHLN1、FGFR2-RABGAP1L、
FGFR2-RABPGAP1L、FGFR2-RASAL2、FGFR2-RASSF4、FGFR2-ROCK1、FGFR2-SH3GLB、FGFR2-
SHC2、FGFR2-SHROOM、FGFR2-SLMAP、FGFR2-SMN1、FGFR2-SOGA1、FGFR2-SORBS1、FGFR2-
SPICE1、FGFR2-STRN4、FGFR2-TFEC、FGFR2-TNS1、FGFR2-TRA2B、FGFR2-TTC28、FGFR2-USP10、
FGFR2-VCL and/or FGFR2-ZYMY4.
In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene
The probe combinations of fusion include the probe selected from following one or more fusions for FGFR3 selected from subordinate list one:
FGFR3-TACC3、FGFR3-BAIAP2L1、TACC3-FGFR3、FGFR3-JAKMIP1、 FGFR3-TNIP2、FGFR3-
WHSC1、FAM193A-FGFR3、FGFR3-ADD1、FGFR3-AES、FGFR3-AMBRA1、FGFR3-ELAVL3、FGFR3-
FBXO28, FGFR3-MLLT10 and/or TEL/ETV6-FGFR3.
In some embodiments, the present invention provides a kind of enrichment human gene FGFR1/2/3(especially FGFR2/3) base
Because merging target calibration method, which comprises extract nucleic acid from the sample of subject, construct nucleic acid library, and use this
The probe combinations of text description capture human gene FGFR1/2/3(especially FGFR2/3) Gene Fusion target, to be enriched with the mankind
Gene FGFR1/2/3(especially FGFR2/3) Gene Fusion target.
In some embodiments, method of the invention can be used for various samples, such as tissue biopsy sample, FFPE sample
Originally, DNA sample and liquid biopsy blood plasma and cfDNA sample.In some embodiments, subject is not particularly limited, can
To include such as normal healthy subjects, the subject with disorders such as cancers is suspected or is diagnosed as.In some embodiments,
Cancer of the present invention is not particularly limited, such as can be relevant to FGFR gene mutation (especially Gene Fusion)
What cancer (including early, middle and late phase cancer), including such as solid tumor as intrahepatic cholangiocarcinoma and bladder transitional cell carcinoma, such as advanced stage
The various samples sources such as advanced stage (III B phase ~ IV phase) intrahepatic cholangiocarcinoma, such as the paraffin embedding fixed through neutral formalin
(FFPE) FGFR1/FGFR2/FGFR3 gene becomes in the Circulating tumor DNA (ctDNA) that tissue samples and plasma sample extract
It is different.
In some embodiments, the present invention is provided to detect human gene FGFR1/2/3(especially FGFR2/3) base
Because of the kit of fusion comprising capture reagent, the capture reagent includes probe combinations described herein.In some embodiment party
In case, the kit may include enrichment human gene FGFR1/2/3(described herein especially FGFR2/3) Gene Fusion
Various reagents used in target calibration method.
In some embodiments, human gene FGFR1/2/3(especially FGFR2/3) Gene Fusion may include
FGFR2-COL14A1, FGFR3-TACC3 and FGFR3-BAIAP2L1.
In some embodiments, the kit further includes one of following or a variety of: 1) building library reagent, such as DNA
Polymerase, ligase, buffer and/or quality-control product, 2) other capture reagents in addition to probe, such as sealer capture buffer
And/or cleaning solution, 3) solid phase carrier, such as magnetic bead and/or 4) high-flux sequence reagent.
As understood by a person skilled in the art, term " probe " refers to the polynucleotide sequence of variable-length, can be used for
The hybridization check of identical, similar or complementary nucleic acid sequence.Sequence as detection probe can mark
Labelling groups, such as radioactive group, fluorophor, chemiluminescent groups or electrochemical luminescence group.Polynucleotide sequence is " mutually
Mend " refer to the sufficient amount of base-pair to match in the sequence and the target sequence generation specific hybrid for being amplified or detecting
Interaction, sequence complementation not necessarily need 100% matched base-pair.Therefore, probe described herein is covered and target sequence
Column are not the corresponding sequences of 100% pairing, including for example have at least 50% with target sequence or its complementary series, 55%, 60%,
65%, the sequence of 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% identity.In some embodiments, spy as described herein
Needle covers the sequence for example hybridized under strict conditions with target sequence.Term " under strict conditions hybridize " under the described conditions that
This at least 60% (65%, 70%, the preferably 75%) hybridization and wash conditions of identical sequence phase mutual cross.This stringent condition
It is known to the skilled in the art, such as can refer to the hybridization in 6X sodium chloride/sodium citrate (SSC) under about 45 °C,
Then 0.2X SSC, 0.1%SDS is used to washed once or for several times under 50-65 °C.Term " hybridization " refers to two polynucleotide sequences
Between complementary A-T and C-G base-pair formation.
In some embodiments, the present invention provides a kind of for analyzing human gene FGFR1/2/3(especially FGFR2/
3) automation equipment of Gene Fusion.In some embodiments, the automation equipment may include library acquisition equipment, institute
State library acquisition equipment and capture human gene FGFR1/2/3(especially FGFR2/3 by probe combinations described herein) gene
Merge target.In some embodiments, the automation equipment can also include high-flux sequence device and/or interpretation of result
Device.In some embodiments, the high-flux sequence device is configured to mankind's base of measurement library capturing unit capture
Because of FGFR1/2/3(especially FGFR2/3) sequence of Gene Fusion target.In some embodiments, the interpretation of result dress
It sets and includes:
Processor;
Memory is stored thereon with instruction, and described instruction executes following one or more steps when being executed by the processor:
1) the human gene FGFR1/2/3(especially FGFR2/3 that measures by the high-flux sequence device is obtained) gene melts
Sequencing data is closed,
2) defining sequencing sequence below is inconsistent reading (discordant reads): Insert Fragment size is greater than
A pair of of reading (reads) of 1000bp or both-end reading (pair end reads) compares onto different chromosomes, or compares knot
Fruit poor direction,
3) defining in all comparison results CIGAR character string containing H and S is editing reading (clipped reads),
4) all inconsistent readings (discordant reads) are extracted and surrounding editing reads (clipped
Reads) and source is marked,
5) duplicate removal is read to above,
6) 27-mer for the reading that source in result above is phase homologous chromosomes is extracted,
7) common 27-mer and blacklist 27-mer are filtered, usually the fusion false positive repetitive sequence on chromosome
8) reading obtained in the previous step comprising above-mentioned 27-mer is clustered using algorithm is merged, is labeled as cluster
(clusters)
9) local assembling (local assembly) is done using fermi-lite,
10) it is compared again to genome is arrived, is filtered simultaneously, only retain the candidate structure variation of phase homologous chromosomes,
11) the inconsistent reading (discordant reads) and surrounding editing that source is different chromosomes are read
(clipped reads) repeats 6) -9) step, retain the candidate structure variation of different chromosomes,
12) two parts result is merged, filtering supports reading (support reads) few as a result, and being annotated.
Detailed description of the invention
Fig. 1 is probe design process example, and wherein Fig. 1 a is FGFR2 introne probe design example: by including
The probe that subregion designs 4x tilling is covered to obtain higher capture rate;Fig. 1 b is by fusion partner gene
The exon of TACC3 and the accuracy for including subregion design probe raising FGFR3 fusion detection.
The overall flow of Fig. 2 display example experiment.
Fig. 3 display example analysis of biological information process.
Specific embodiment
The purpose of the present invention is to provide the probe sequences and the method for salary distribution of detection FGFR1/2/3 Gene Fusion.This hair
The bright FGFR1/2/3 gene that has been directed to devises specific aim primer, and finds that the primer can be with highly sensitive and specificity
Extensive FGFR1/2/3 Gene Fusion is detected, especially common FGFR1/2/3 Gene Fusion.In further implementation of the invention
In scheme, all FGFR1/2/3 Gene Fusions of the primer combined covering that the present invention designs, thus realize disposably detect it is all can
The purpose of energy FGFR1/2/3 Gene Fusion.
Target gene:
FGFR1(NM_001174063): whole exons and 4,5,7,9, No. 17 intrones;
FGFR2(NM_001144917): whole exons and 1,15, No. 17 intrones;
FGFR3(NM_000142): whole exons and No. 17,18 intrones and 3 ' UTR;
FGFR3 chaperone TACC3(NM_006342) the 11st, No. 14 probe covering for including subregion.
Local scope: the appearance of target gene part first is outer aobvious in the upper all fusion forms being likely to occur of COSMIC
The introne of sub- front.
Probe length: 120 nt are greater than length in the region of probe length, are completely covered by the way of probe overlapping
Target area, the region of probe overlapping are 60 nt.It is less than the region of probe length for length, is covered completely using single probe
Lid target area, the center of probe are overlapped with the center in region.For merging hot spot region, by including sub-district
The probe that 4x tilling is designed in domain is covered to obtain higher capture rate.Every probe all passes through BLAST and full-length genome
It compares;The hit for being 60 or more for length carries out score calculating;Every probe calculates total score according to the quantity of hit, and score is higher
Probe specificity it is poorer.Probe of the score greater than 2 is not considered.The single probe combinations in region region may probe combinations
In the minimum probe groups of all probe score summations.It does not consider in the region consensus repeat (such as SINE, LINE, LTR etc.)
Design probe.For the genome repeat region where FGFR fusion, it is difficult to probe is directly designed, by fusion partner
(partner) gene, for example, TACC3 exon and include subregion design probe improve FGFR3 fusion detection it is accurate
Degree.Probe combinations appropriate are selected, and are verified, subordinate list one is shown in.Probe design process example is shown in Fig. 1.
The present invention also mentions the kit of detection FGFR1/2/3 Gene Fusion, especially disposably detects FGFR1/2/3 gene
The kit of fusion.This kit is mainly used for gene DNA variation detection, and the exemplary compositions of kit are as shown in the table.
Kit exemplary group is at (kit may include following one or more reagents, and can be placed in one or more
In a packaging).
The method of the present invention may include that following one or more stages (and the stage can be suitble to make for packet of each stage
Corresponding reagent, the reagent can be selected by those skilled in the art, may include various commercialization reagents or reagent
Box): nucleic acid extraction, library construction, library capture, the sequencing of upper machine, interpretation of result stage.The overall flow of exemplary experiment is shown in figure
2.It is specific illustrative steps are as follows be described in detail:
(1) the genomic DNA double-strandednucleic acid in blood and tissue samples is extracted;
(2) to handle to obtain DNA for the DNA double chain nucleic acid denaturation of step (1) single-stranded, carries out end reparation, link and PCR and expands
Library is built in increasing;
(3) after building library, the DNA library is captured using capture probe described in claim;
(4) it requires to carry out upper machine testing on Fit Models according to sequencing reaction universal kit;
(5) data after sequencing are subjected to automatic processing, remove background noise, the biological information merged according to said gene
Analysis method is learned, the Gene Fusion of sample is detected.
This kit can theoretically detect all known and unknown chaperone including FGFR1/2/3 gene
Fusion, representative Gene Fusion information such as following table.
Probe of the invention, method, the FGFR1/2/3 representativeness Gene Fusion of product testing.
The present invention also provides a kind of detection FGFR1/2/3 Gene Fusions (including disposably to detect FGFR1/2/3 gene and melt
Close) bioinformatic analysis method, specific illustrative method is as shown in Figure 3.
It is verified that this invention advantageously has following one or more effects:
1) the encoded exon region in the region genome target gene FGFR1/2/3 of capture probe capture human genome and outer aobvious
Sub- introne handover region, specificity are high;Reduce non-specific capture;
2) sample initial amount is low, and sensibility is high;Gene mutation of the content down to 2% in detectable 50 ng fragmentation DNA samples, or
In plasma sample down to 0.7% gene mutation;
3) current all FGFR1/2/3 Gene Fusions are applicable to, do not limit fusion partner gene in upstream or downstream, companion
Which companion's gene is, it is possible to find the either totally unknown fused type of the unknown fusion breakpoint of known chaperone;
4) sample type on probation is wide, including but not limited to the patients with solid tumor paraffin embedding (FFPE) fixed through neutral formalin
Tissue samples and plasma sample extract plasma DNA (cell free DNA, cfDNA), wherein include circulating tumor
DNA(Circulating tumor DNA, ctDNA).
Exemplary description hereafter carried out respectively to five stages of the method for inspection of the invention: nucleic acid extraction, library construction,
Library capture, the sequencing of upper machine and interpretation of result.
A. nucleic acid extraction
1.1 illustrate that the extraction for carrying out plasma sample nucleic acid and purifying (recommend nucleic acid to mention according to producer using nucleic acid extraction kit
Reagent is taken, see Table 2 for details), cfDNA(cell-free DNA is all referred to as below the plasma DNA after extraction);
The cfDNA of 1.2 pairs of extractions carries out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument);(after the completion of this step can in
Disconnected experiment is extracted product and is saved in -20 ± 5 DEG C, -70 DEG C or less long-term preservations.)
B. library construction
2. preparing before experiment
2.1 taking-up ends repair buffer and are placed in normal temperature unfreezing, and it is spare that end repair enzyme is placed in ice chest;
2.2 prepare enough Fresh 80% ethyl alcohol it is spare (80% ethyl alcohol total amount of Fresh is prepared by the every sample of 1mL, such as
12 samples build library and prepare 12mL80% alcohol);
2.3 gDNA fragmentations (only limit FFPE sample): taking 50-500 ng gDNA in 1.5ml centrifuge tube, can less than 50 μ L
50ul is complemented to nuclease-free water, of short duration centrifugation after mixing;50 μ L gDNA solution are transferred to interrupt pipe and be put into and are interrupted in instrument,
Fragment length is selected to carry out gDNA fragmentation for the program that interrupts of 200-300bp;(after the completion of this step can interrupt experiments, produce
Object freezes in 20 ± 5 DEG C, 70 DEG C or less long-term preservations;
3. end is repaired
3.1 (will can prepare mixed in the PCR reaction tube or eight connecting legs of following components addition 0.2ml nuclease free when sample is more
It closes liquid to be dispensed);
3.2 concussions mix, of short duration centrifugation, and following procedure is run in PCR instrument, and (negative quality-control product and positive quality control product are from this step
It is rapid to start to operate with sample synchronization):
4. connection
4.1 take out previous step reaction tube, and gently concussion mixes and reaction tube is added by following components after of short duration centrifugation:
4.2 concussions mix, and following procedure is run in PCR instrument after of short duration centrifugation:
4.3 take out magnetic bead A, and equilibrium at room temperature 30min is spare;
4.4 take out reaction tube, and 43 μ L magnetic bead A are added, is sufficiently transferred in 1.5ml centrifuge tube after piping and druming, is incubated at room temperature 5min;
4.5 are transferred to centrifuge tube on magnetic frame, after standing 2min, clarify careful inhale to solution and abandon supernatant;
4.6 are added 200 μ L, 80% ethyl alcohol, cover after centrifuge tube lid is stored at room temperature 30s, careful inhale abandons supernatant;
4.7 repeat 4.6 operations once, it is ensured that tube wall and tube bottom no liquid residual;
4.8 are maintained at centrifuge tube on magnetic frame, and drying at room temperature 5min(can not make magnetic bead over-drying);
4.9 21 μ L of addition are stored at room temperature 5min after being resuspended without the concussion of enzyme water;
4.10 are transferred to centrifuge tube on magnetic frame, and after being stored at room temperature 2min, it is anti-to new 0.2ml PCR to draw 20 μ L supernatants
Ying Guanzhong;
5. PCR amplification
5.1 choose corresponding sample label and record, and 5 μ L sample labels are added separately into PCR reaction tube, and carry out
Label;
5.2 are added 25 μ L PCR reaction solutions I, mix well, run following procedure in PCR instrument after of short duration centrifugation:
5.3 take out PCR reaction tube, and 50 μ L magnetic bead A are added, and room temperature after mixing in 1.5ml centrifuge tube is sufficiently transferred to after piping and druming and is incubated
Educate 5min;
5.4 are transferred to centrifuge tube on magnetic frame, and after being stored at room temperature 2min, careful inhale abandons supernatant;
5.5 are added 200 μ L, 80% ethyl alcohol, and after being stored at room temperature 1min, careful inhale abandons supernatant;
5.6 repeat 5.5 operations once, it is ensured that tube wall and tube bottom no liquid residual;
5.7 are maintained at centrifuge tube on magnetic frame, and drying at room temperature 5min(can not make magnetic bead over-drying);
5.8 31 μ L of addition are stored at room temperature 5min after being resuspended without the concussion of enzyme water;
5.9 are transferred to centrifuge tube on magnetic frame, after being stored at room temperature 2min, draw supernatant into new 1.5ml centrifuge tube;
5.10 pairs of library nucleic acids carry out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument);It (can be interrupted after the completion of this step
Experiment, product are saved in -20 ± 5 DEG C, -70 DEG C or less long-term preservations).
C. library captures
6. capture
6.1 are carried out calculating taken amount and be mixed into the centrifuge tube of new 1.5ml by following principle to DNA library:
1) library of four different sample labels is recommended to blend together a mixing library;
2) library of concentration comparable suggests being arranged in a mixing library, avoids hybridization efficiency difference;
3) total amount in a mixing library is 1.6 μ g, and each library amount mean allocation is (if sample less than 4, guarantees its mixing
Library total amount is 1.6 μ g afterwards);5 μ L sealers I, 2 μ L sealers II are added into each mixing library respectively, it is sufficiently mixed
To be centrifuged nozzle with ParafilmTM after even of short duration centrifugation, and 5 apertures are pierced on sealed membrane with clean pipette tips, is put into vacuum
Concentrating instrument, vacuum are drained to no liquid and are remained;
6.2 sequentially added into the mixing library after draining 8.5 μ L capture buffer, 2.7 μ L capture reinforcing agent and 3.8 μ L without
Mixing fullys shake in enzyme water, and after of short duration centrifugation, concussion centrifugation is primary when 5min during room temperature is protected from light standing 10min() it is heavy molten;
6.3 are fully transferred to liquid in 0.2mLPCR reaction tube, are placed in 95 DEG C of denaturation 10min in PCR instrument;
6.4 take out PCR reaction tube, mix well after rapidly joining 2 μ L capture probes, of short duration centrifugation is incubated for 65 DEG C in PCR instrument
Educate (time is no more than 18 hours overnight, and heat lid is set as 75 DEG C) overnight;
6.5 take a certain amount of magnetic bead cleaning solution (2 ×), cleaning solution I (10 ×), cleaning solution II (10 ×), cleaning solution III (10 ×),
Cleaning solution IV (10 ×) calculates demand as described below, and it is spare to be diluted to corresponding 1 × working solution respectively with no enzyme water;
6.6 take a certain amount of (by 100 μ L × mixing library number calculating demand) 1 from 1 × working solution after dilution in advance × it washes
It washs liquid I and all 1 × cleaning solution II is spare in 65 DEG C of metal baths incubation 2h or more;
6.7 take magnetic bead B to be stored at room temperature 15-30min in advance;
6.8 concussions take out a certain amount of (by 100 μ L × mixing library number calculating demand) magnetic bead B to new immediately after being resuspended
It in 1.5mL centrifuge tube, is transferred on magnetic frame and stands 1min, inhale and abandon supernatant;
6.9 are added 1 × magnetic bead cleaning solution of 2 times of volumes, and resuspension fullys shake, is transferred on magnetic frame and stands after of short duration centrifugation
1min inhales and abandons supernatant;
6.10 repeat 6.9 once;
6.11 are added 1 × magnetic bead cleaning solution of 1 times of volume, are dispensed into different 0.2mL according to every 100 μ L of pipe after concussion resuspension
In PCR reaction tube;
PCR reaction tube is transferred on the magnetic frame of 0.2ml specification and stands 1min by 6.12, and careful inhale abandons supernatant, and pipette tips is avoided to inhale
To magnetic bead;
6.13 take out the PCR reaction tube after overnight hybridization, and it is anti-that whole hybrid products are transferred to the above-mentioned PCR containing magnetic bead B
Ying Guanzhong, of short duration centrifugation is placed on 65 DEG C of incubation 45min in PCR instrument after sufficient vortex mixing, is during which vortexed and mixes every 10min
Once magnetic bead is avoided to be settled down to bottom;
After 6.14 are incubated for, 1 × cleaning solution I of 65 DEG C of 100 μ L preheatings is added in every pipe, and whole suspensions are transferred to 1.5mL
In centrifuge tube (being placed on 65 degree of metal baths for 5 minutes in advance), concussion is mixed, and after of short duration centrifugation, is transferred on magnetic frame and is stood 5s,
It inhales and abandons supernatant;
6.15 are added 1 × cleaning solution II of 65 DEG C of 200 μ L preheatings, and concussion mixes, and 65 DEG C of incubations are immediately placed on after of short duration centrifugation
5min is then transferred on magnetic frame and stands 5s, inhales and abandons supernatant.It is primary to repeat this step;
6.16 are added 200 1 × cleaning solutions of μ L I, and of short duration centrifugation after vortex 2min is then allowed to stand on magnetic frame, magnetic bead is complete
Remove supernatant after separation;
6.17 are added 200 1 × cleaning solutions of μ L III, and of short duration centrifugation after vortex 1min is then allowed to stand on magnetic frame, magnetic bead is complete
Remove supernatant after separation;It is added 200 1 × cleaning solutions of μ L IV, of short duration centrifugation after vortex 30s is then allowed to stand on magnetic frame, magnetic
Pearl removes supernatant after being kept completely separate;
6.18 18 μ L of addition are fully transferred to spare in 0.2mL PCR reaction tube after being resuspended without the concussion of enzyme water;
6.19 are separately added into 25 μ L PCR reaction solutions and 5 μ L PCR primers into above-mentioned PCR reaction tube, mix well it is of short duration from
Following procedure is run after the heart in PCR instrument:
6.20 take out magnetic bead A from four degree of refrigerators, and equilibrium at room temperature 30min is spare.After PCR take out PCR reaction tube, it is of short duration from
After the heart, product is fully transferred in new 1.5mL centrifuge tube;
6.21 are added 75 μ L magnetic bead A, and concussion mixes, is stored at room temperature of short duration centrifugation after 10min, is transferred on magnetic frame and stands
1min inhales and abandons supernatant;
6.22 are added 200 μ L, 80% ethyl alcohol, after standing 30s, inhale and abandon supernatant.It is primary to repeat this step operation;
6.23 uncap dry to dripless residual and magnetic bead it is non-reflective;
6.24, which are added 22 μ L, shakes without enzyme water magnetic bead is resuspended, and of short duration centrifugation, which is stored at room temperature after 5min to be transferred on magnetic frame, to be stood
It is spare into new 1.5mL centrifuge tube to draw 20 μ L supernatants by 1min;
6.25 take 1 μ L capture library to carry out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument);(after the completion of this step can in
Disconnected experiment, product freeze in -20 ± 5 DEG C, -70 DEG C or less long-term preservations.)
D. upper machine sequencing
7. machine is sequenced on
7.1 require to carry out on Fit Models according to sequencing reaction universal kit (PCR sequencing PCR) (providing reagent for oneself, see Table 2 for details)
Upper machine testing;
E. interpretation of result
8. result obtains and analysis
Data after sequencing are transmitted to mankind's FGFR1/2/3 mutation combinations and test and analyze software, runs software is compared point
Sample variation result is obtained after analysis.Specific step is as follows for raw letter analysis process:
8.1 definition sequencing sequences below are inconsistent reading: Insert Fragment size is greater than 1000bp or both-end is read
A pair of of read-around ratio to in different chromosomes or comparison result poor direction,
CIGAR character string is editing reading containing H and S in 8.2 all comparison results of definition
8.3 all inconsistent readings of extraction and surrounding editing read and mark source;
8.4 pairs or more read duplicate removal
8.5 extract the 27-mer that source in result above is phase homologous chromosomes
8.6 filterings common 27-mer and blacklist 27-mer
8.7 cluster the reading obtained in the previous step comprising above-mentioned 27-mer using union-find algorithm
(cluster), it is labeled as cluster
8.8 do partial assembled using fermi-lite software
8.9 compare again to genome, only retain the candidate structure variation of phase homologous chromosomes
8.10 pairs of sources are that the inconsistent and editing reading of different chromosomes repeats 8.5-8.9 step, retain different chromosomes
Candidate structure variation
8.11 merge two parts result, filter, annotation;
8.12 refer to genome and database, and the current reference genome of raw letter analysis process is based on USCS hg19, version
This number Version no.:v1.0 (20-Mar-2009 version).Database includes:
1) panel internal reference genome and common SNP 27-mer data file
2) the 27-mer data file introduced by experimentation and sequencing procedure
3) comment file is merged: the GTF file converted using UCSC hgTables 2013-06-14 editions
9. fused data analyzes result judgement:
9.1 analytical parameter setups are as follows:
10. fusion software performance comparative analysis:
10.1 samples selections and experimentation:
Using totally 10,75x2 sample of UDI test, three tools distinguish call SV.Filter condition: autofusion takes existing
It has ready conditions.Delly2 uses PASS and PRECISE tag as filter condition, requires breakpoint distance in the SV of DEL, INV type
Greater than 1000, the filter criteria after annotation is consistent with autofusion.BreakID does not need filter condition;
10.2 experimentation:
The version of BreakID downloading can not compile, and modify header file and compiling condition while can run after modifying partial code
But all samples are all without any result.It is final the result is that using recognizing after IGV interpretation after result after all filterings is merged
For the result for being true positives;BreakID not positive reason analysis: the SA tag of this tool dependence bwa mem;It is required that breakpoint
There is SA tag at both ends, it is more likely that require to bwa version and existing procedure is incompatible;
10.3 experimental result:
True positives 8, the sensibility and PPV of existing autofusion is all 100%:
11. detection limit (LOD) analysis
Probe sequence is merged using the FGFR1/2/3 of the covering of targeted capture panel described in subordinate list one, is carried out using two cell lines
Test, wherein A has FGFR2-COL14A1 and FGFR3-TACC3 fusion, another B has FGFR3-BAIAP2L1 fusion.Mixing ladder
Degree is following to be indicated, each 15-20 sample of gradient differs.Gradient concentration is quantified by digital pcr:
Using 95% recall rate as standard, current fusion LOD is about 0.7%, and detailed results are as shown in the table:
The specificity of probe combinations and method detection FGFR Gene Fusion of the present invention and sensitivity are significantly higher than existing method at present
And probe combinations.
The detection limit performance of the present invention and prior art detection Gene Fusion is referring to following table:
Name of product | Manufacturer | The fusion declared | Detection limit performance indicator |
10 gene mutation combined detection kit of the mankind (reversible end termination PCR sequencing PCR) | Xiamen Ai De | ALK, ROS1, RET | The detection of 30ng DNA sample is fused to variation abundance 1% |
Human epidermal growth factor receptor/ALK/BRAF/KRAS gene mutation combined detection kit (reversible end End terminates PCR sequencing PCR) | Fire stone in Guangzhou | ALK | The detection ALK fusion detection of 50ng DNA sample is limited to variation abundance 10% |
EGFR/ALK/ROS1/BRAF/KRAS/HER2 gene mutation detection kit (can Inverse end terminates PCR sequencing PCR) | Nanjing generation and | ALK, ROS1 | 250ng DNA sample detection ROS1 is fused to variation abundance 1%, ALK is fused to variation abundance 2.5% |
Human epidermal growth factor receptor, KRAS, BRAF, PIK3CA, ALK, ROS1 gene mutation detection kit (semiconductor PCR sequencing PCR) | Tianjin promise standing grain | ALK, ROS1 | 30ng DNA detection fusion detection is limited to 100 copies |
Mankind's FGFR1/2/3 gene mutation combined detection kit (high-flux sequence method) (present invention) | Thinking enlightening | FGFR1,FGFR2, FGFR3 | 30-60ng cfDNA or 50-200 ng gDNA detects FGFR Fusion detection is limited to variation abundance 0.7% |
In summary, the present invention provides a set of high-flux sequences to detect human gene FGFR1, FGFR2, and FGFR3 gene melts
Capture probe, detection method and kit and application are closed, has biggish promotion in performance, testing result shows of the invention
Detection architecture disposably can accurately detect the fusion variation of FGFR1/2/3 gene, and detection limit can be down to 0.7%.It therefore also can be wide
It is general to be suitable for various samples, including such as tumor tissue biopsy sample, FFPE sample, DNA sample and liquid biopsy blood plasma,
The detection of cfDNA sample.
The FGFR1/2/3 of one targeted capture panel of subordinate list covering merges probe sequence
Note: genomic data version number is UCSC hg19, and the Genbank number of corresponding genome version is GCA_
000001405.1。
Exemplary embodiments of the invention are illustrated through the foregoing embodiment.The embodiment is to each technical side of the present invention
Case does not constitute a limitation.It should be understood by those skilled in the art that: it can modify to the technical solution of foregoing embodiments, or
Equivalent substitution of some or all of the technical features.These modify or replace the model still in technical solution of the present invention
In enclosing.
Sequence table
<110>Co., Ltd, Shanghai thinking enlightening medical test institute
<120>FGFR Gene Fusion probe, detection method and kit
<130> IB191545
<160> 624
<170> PatentIn version 3.3
<210> 1
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 1
cttgtcagag ggcaccacag agtccattat gatgctccag gtggcataac ggacctgagg 60
ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 120
<210> 2
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 2
ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 60
ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 120
<210> 3
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 3
ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 60
gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 120
<210> 4
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 4
gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 60
ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 120
<210> 5
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 5
ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 60
ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 120
<210> 6
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 6
ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 60
acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 120
<210> 7
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 7
acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 60
catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 120
<210> 8
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 8
catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 60
aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 120
<210> 9
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 9
aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 60
actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 120
<210> 10
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 10
actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 60
atgtagtgag tgcatggtga attaatttaa aatatttctg gctgggtgcg gtggctcaag 120
<210> 11
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 11
gtgagactcc gtctaaaaaa aaaaaaaaaa tgcttacacc ctttaagtta gtaatttcac 60
ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 120
<210> 12
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 12
ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 60
cattatttat tatggtgaaa aaccagaaaa tacctaatat cacaaaatag ttcattaaat 120
<210> 13
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 13
gcccagccaa attatgggat tttaaaataa tggaatctta tacagtcatg aaaaattatg 60
tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 120
<210> 14
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 14
tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 60
aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 120
<210> 15
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 15
aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 60
gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 120
<210> 16
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 16
gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 60
gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 120
<210> 17
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 17
gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 60
ggcctcccct gttcccatta ctctaacttt cgcatgcaca cacacgtacc ttgtagcctc 120
<210> 18
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 18
acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 60
ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 120
<210> 19
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 19
ctgtgcctgg cctatggccc cttctcttaa tcaccactca ctcacaactt cagtctgaag 60
taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 120
<210> 20
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 20
acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 60
agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 120
<210> 21
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 21
ccactgcgcc cggccttctt tcccttttac aagtacagaa caagtggact tcaaaagtcc 60
aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 120
<210> 22
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 22
ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 60
accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 120
<210> 23
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 23
cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 60
gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 120
<210> 24
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 24
cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 60
gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 120
<210> 25
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 25
acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 60
accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 120
<210> 26
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 26
ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 60
atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 120
<210> 27
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 27
tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 60
acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 120
<210> 28
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 28
acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 60
ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 120
<210> 29
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 29
accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 60
ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 120
<210> 30
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 30
ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 60
cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 120
<210> 31
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 31
taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 60
ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 120
<210> 32
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 32
tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 60
ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 120
<210> 33
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 33
gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 60
gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 120
<210> 34
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 34
gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 60
tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 120
<210> 35
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 35
atctctttgt cggtggtatt aactccagca gtctagaaga gacaacggaa gcaaaatgga 60
caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 120
<210> 36
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 36
tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 60
gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 120
<210> 37
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 37
tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 60
aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 120
<210> 38
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 38
gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 60
accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 120
<210> 39
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 39
gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 60
cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 120
<210> 40
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 40
tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 60
agactggtgc catgattacc ttcaagatct ggacataagg caggttgtct gggccaatct 120
<210> 41
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 41
accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 60
gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 120
<210> 42
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 42
acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 60
agggaatgcc taaccccttc tctctctttt ttaagagatg gggtaccatt ctgtcaaccc 120
<210> 43
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 43
tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 60
acagtgtctc acgcatacgg tttggtttgg tgttatctgt ttctttctcc tctgaagagg 120
<210> 44
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 44
ctgcccgggg aaagccaaga gagacaggca gggtggagag gagcagctgg tcaggctcag 60
gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 120
<210> 45
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 45
ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 60
ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 120
<210> 46
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 46
atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 60
ccatctactt tctgttacct gtctgcgcag agggatgctc ttggccagct tgtgcacagc 120
<210> 47
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 47
ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 60
tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 120
<210> 48
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 48
agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 60
tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 120
<210> 49
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 49
atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 60
acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 120
<210> 50
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 50
gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 60
gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 120
<210> 51
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 51
tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 60
gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 120
<210> 52
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 52
tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 60
tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 120
<210> 53
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 53
gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 60
tcccgtgggg aagggtgggc acacagggga caagggtatg gtatggctga gatggtttgg 120
<210> 54
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 54
caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 60
gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 120
<210> 55
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 55
accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 60
acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 120
<210> 56
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 56
tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 60
attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 120
<210> 57
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 57
cagcagtgtg aaaatggact aatacaaaca gttctcttct ctaccctgca tctctgcact 60
tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 120
<210> 58
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 58
tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 60
acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 120
<210> 59
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 59
ggccgaacca gaagaacccc agagttcatg gatgcactgg agtcagcaga cacctgcaag 60
gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 120
<210> 60
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 60
ctgtgatggg cgagaggaag cagcgatggg ccgggcccct cctccctgct cagggaggtg 60
cgtgcacgca gtggggacgg cctgagctct ggctctggca cgggcagcct tacctggttg 120
<210> 61
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 61
gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 60
acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 120
<210> 62
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 62
ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 60
ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 120
<210> 63
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 63
ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 60
tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 120
<210> 64
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 64
aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 60
tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 120
<210> 65
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 65
gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 60
gatttattac ctttcccttt tagttttttt tttttttttt ttttttaacg cagagtctca 120
<210> 66
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 66
aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 60
tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 120
<210> 67
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 67
gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 60
tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 120
<210> 68
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 68
gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 60
tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 120
<210> 69
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 69
attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 60
atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 120
<210> 70
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 70
aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 60
ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 120
<210> 71
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 71
tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 60
aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 120
<210> 72
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 72
gtgagtgctg gctctggcct ggtgccaccc gcctatgccc ctccccctgc cgtccccggc 60
catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 120
<210> 73
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 73
ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 60
ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 120
<210> 74
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 74
tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 60
ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 120
<210> 75
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 75
tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 60
tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 120
<210> 76
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 76
cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 60
ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 120
<210> 77
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 77
ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 60
cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 120
<210> 78
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 78
tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 60
caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 120
<210> 79
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 79
agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 60
ggagtacctg gacctgtcgg cgcctttcga gcagtactcc ccgggtggcc aggacacccc 120
<210> 80
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 80
acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 60
gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 120
<210> 81
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 81
ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 60
atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 120
<210> 82
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 82
gacgtgaagg gccactggtc cccaacaatg tgaggggtcc ctagcagccc accctgctgc 60
tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 120
<210> 83
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 83
ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 60
tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 120
<210> 84
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 84
gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 60
ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 120
<210> 85
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 85
tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 60
ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 120
<210> 86
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 86
cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 60
ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 120
<210> 87
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 87
tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 60
tttgttaacc cagcgacgaa ctttccgaaa aataaagaca cctggttgct aacctggccc 120
<210> 88
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 88
ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 60
acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 120
<210> 89
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 89
atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 60
gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 120
<210> 90
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 90
acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 60
ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 120
<210> 91
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 91
caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 60
cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 120
<210> 92
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 92
ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 60
tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 120
<210> 93
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 93
ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 60
tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 120
<210> 94
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 94
gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 60
aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 120
<210> 95
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 95
aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 60
ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 120
<210> 96
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 96
catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 60
agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 120
<210> 97
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 97
ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 60
acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 120
<210> 98
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 98
ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 60
tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 120
<210> 99
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 99
aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 60
tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 120
<210> 100
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 100
caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 60
agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 120
<210> 101
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 101
gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 60
cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 120
<210> 102
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 102
ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 60
cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 120
<210> 103
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 103
aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 60
gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 120
<210> 104
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 104
gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 60
aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 120
<210> 105
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 105
ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 60
ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 120
<210> 106
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 106
tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 60
tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 120
<210> 107
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 107
tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 60
tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 120
<210> 108
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 108
cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 60
tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 120
<210> 109
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 109
agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 60
ctacgcatgt ctcacaagac aaccaaggac aaggggcttc tagaaggaag ttcttacctc 120
<210> 110
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 110
tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 60
agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 120
<210> 111
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 111
aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 60
tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 120
<210> 112
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 112
caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 60
tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 120
<210> 113
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 113
cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 60
gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 120
<210> 114
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 114
gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 60
ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 120
<210> 115
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 115
agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 60
caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 120
<210> 116
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 116
caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 60
attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 120
<210> 117
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 117
gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 60
ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 120
<210> 118
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 118
gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 60
ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 120
<210> 119
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 119
ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 60
cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 120
<210> 120
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 120
acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 60
cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 120
<210> 121
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 121
tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 60
ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 120
<210> 122
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 122
attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 60
caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 120
<210> 123
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 123
gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 60
ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 120
<210> 124
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 124
ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 60
ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 120
<210> 125
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 125
tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 60
tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 120
<210> 126
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 126
ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 60
agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 120
<210> 127
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 127
ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 60
gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 120
<210> 128
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 128
caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 60
ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 120
<210> 129
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 129
atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 60
aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 120
<210> 130
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 130
gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 60
ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 120
<210> 131
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 131
cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 60
tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 120
<210> 132
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 132
tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 60
taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 120
<210> 133
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 133
aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 60
cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 120
<210> 134
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 134
attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 60
taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 120
<210> 135
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 135
cactaaagga aagagattgg cgagtcaggg aatcttcccc aatgccaaat cactccagcc 60
caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 120
<210> 136
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 136
accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 60
gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 120
<210> 137
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 137
cctgcctcgg cctcccaaag tgctgggatt acaggcatga gccactgcgc ctgaccaact 60
tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 120
<210> 138
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 138
ggtgaatact gttcgagagg ttggctgagg tccaagtatt cctgaaagaa gggaagagag 60
acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 120
<210> 139
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 139
gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 60
aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 120
<210> 140
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 140
gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 60
cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 120
<210> 141
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 141
cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 60
tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 120
<210> 142
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 142
aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 60
tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 120
<210> 143
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 143
acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 60
ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 120
<210> 144
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 144
agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 60
cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 120
<210> 145
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 145
ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 60
ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 120
<210> 146
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 146
ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 60
ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 120
<210> 147
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 147
tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 60
acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 120
<210> 148
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 148
gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 60
tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 120
<210> 149
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 149
tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 60
gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 120
<210> 150
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 150
cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 60
atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 120
<210> 151
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 151
ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 60
aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 120
<210> 152
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 152
agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 60
tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 120
<210> 153
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 153
gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 60
agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 120
<210> 154
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 154
taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 60
accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 120
<210> 155
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 155
gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 60
cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 120
<210> 156
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 156
agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 60
acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 120
<210> 157
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 157
aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 60
attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 120
<210> 158
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 158
tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 60
ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 120
<210> 159
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 159
taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 60
tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 120
<210> 160
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 160
ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 60
atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 120
<210> 161
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 161
tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 60
aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 120
<210> 162
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 162
ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 60
cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 120
<210> 163
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 163
tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 60
agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 120
<210> 164
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 164
tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 60
gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 120
<210> 165
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 165
cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 60
aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 120
<210> 166
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 166
cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 60
gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 120
<210> 167
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 167
atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 60
gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 120
<210> 168
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 168
acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 60
gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 120
<210> 169
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 169
gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 60
tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 120
<210> 170
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 170
tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 60
tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 120
<210> 171
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 171
agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 60
cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 120
<210> 172
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 172
gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 60
gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 120
<210> 173
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 173
tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 60
cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 120
<210> 174
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 174
gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 60
acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 120
<210> 175
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 175
ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 60
agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 120
<210> 176
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 176
cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 60
ggcgaagctc cccgaggcgt cttgcgcggc gattaccttg aatggcaacg ctcctccgcg 120
<210> 177
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 177
caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 60
gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 120
<210> 178
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 178
tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 60
acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 120
<210> 179
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 179
ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 60
cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 120
<210> 180
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 180
tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 60
gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 120
<210> 181
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 181
acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 60
ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 120
<210> 182
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 182
aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 60
acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 120
<210> 183
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 183
ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 60
caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 120
<210> 184
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 184
cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 60
taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 120
<210> 185
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 185
tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 60
ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 120
<210> 186
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 186
acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 60
ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 120
<210> 187
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 187
agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 60
cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 120
<210> 188
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 188
ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 60
ttattatttt ctttttgaga tgaagtcttg ctctgtctcc cagactggag tgcagtggcg 120
<210> 189
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 189
cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 60
caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 120
<210> 190
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 190
aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 60
ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 120
<210> 191
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 191
agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 60
aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 120
<210> 192
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 192
ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 60
tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 120
<210> 193
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 193
catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 60
tttttttttt ttttgagacg gagtctcgct ctgtcgccaa ggctggaatg caatggcatg 120
<210> 194
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 194
cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 60
gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 120
<210> 195
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 195
ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 60
tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 120
<210> 196
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 196
aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 60
aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 120
<210> 197
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 197
ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 60
gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 120
<210> 198
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 198
cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 60
acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 120
<210> 199
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 199
attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 60
cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 120
<210> 200
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 200
cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 60
tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 120
<210> 201
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 201
tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 60
tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 120
<210> 202
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 202
cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 60
ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 120
<210> 203
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 203
cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 60
ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 120
<210> 204
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 204
acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 60
gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 120
<210> 205
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 205
ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 60
cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 120
<210> 206
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 206
cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 60
ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 120
<210> 207
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 207
tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 60
agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 120
<210> 208
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 208
acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 60
tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 120
<210> 209
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 209
atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 60
gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 120
<210> 210
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 210
tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 60
aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 120
<210> 211
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 211
tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 60
agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 120
<210> 212
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 212
ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 60
catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 120
<210> 213
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 213
tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 60
tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 120
<210> 214
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 214
taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 60
attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 120
<210> 215
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 215
ccctcctcgg cttcccaaaa tgctgggatt acaggcgaga gcataaatat tttagggaaa 60
ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 120
<210> 216
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 216
gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 60
cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 120
<210> 217
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 217
ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 60
atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 120
<210> 218
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 218
ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 60
tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 120
<210> 219
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 219
tcaccactca ctcacaactt cagtctgaag taccatttgt aacacagcaa aaccgtgtcc 60
ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 120
<210> 220
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 220
caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 60
tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 120
<210> 221
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 221
aagtacagaa caagtggact tcaaaagtcc aaagcatttg attaaaaaaa aaaaaaaaaa 60
aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 120
<210> 222
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 222
cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 60
ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 120
<210> 223
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 223
gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 60
gcccagggag cgaagggaga tgttaagtga gatttattac ctttcccttt tagttttttt 120
<210> 224
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 224
aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 60
ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 120
<210> 225
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 225
ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 60
acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 120
<210> 226
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 226
gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 60
atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 120
<210> 227
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 227
tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 60
ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 120
<210> 228
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 228
ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 60
aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 120
<210> 229
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 229
acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 60
tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 120
<210> 230
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 230
tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 60
aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 120
<210> 231
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 231
ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 60
cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 120
<210> 232
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 232
tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 60
gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 120
<210> 233
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 233
ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 60
cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 120
<210> 234
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 234
ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 60
attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 120
<210> 235
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 235
gtctagaaga gacaacggaa gcaaaatgga caagcacagg acatgagacc tctaagagac 60
gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 120
<210> 236
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 236
attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 60
ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 120
<210> 237
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 237
catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 60
tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 120
<210> 238
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 238
cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 60
ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 120
<210> 239
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 239
cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 60
gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 120
<210> 240
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 240
tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 60
ggacataagg caggttgtct gggccaatct tgctcccatt cacctcgatg tgctttagcc 120
<210> 241
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 241
ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 60
ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 120
<210> 242
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 242
gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 60
tgttatctgt ttctttctcc tctgaagagg agtcatcatc atcatcatca tcctccgagg 120
<210> 243
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 243
gggtggagag gagcagctgg tcaggctcag gagcagggcc caggccagga cctggaggtg 60
tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 120
<210> 244
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 244
tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 60
tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 120
<210> 245
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 245
gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 60
agggatgctc ttggccagct tgtgcacagc catctggctg tggaagtcac tcttcttggt 120
<210> 246
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 246
attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 60
tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 120
<210> 247
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 247
tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 60
ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 120
<210> 248
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 248
atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 60
ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 120
<210> 249
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 249
tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 60
gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 120
<210> 250
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 250
tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 60
gcacacacct gtgccatctg taccatttcc tcccgtgggg aagggtgggc acacagggga 120
<210> 251
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 251
ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 60
tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 120
<210> 252
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 252
gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 60
cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 120
<210> 253
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 253
ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 60
ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 120
<210> 254
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 254
aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 60
aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 120
<210> 255
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 255
gttctcttct ctaccctgca tctctgcact tctaagcagc ccttggggac agttcaaatt 60
catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 120
<210> 256
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 256
caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 60
gcatacacag ccagcagatg cgagtatgca agggaatgcc taaccccttc tctctctttt 120
<210> 257
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 257
gatgcactgg agtcagcaga cacctgcaag gaagagtggg gtcaccctag agcaaggagg 60
ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 120
<210> 258
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 258
ccgggcccct cctccctgct cagggaggtg cgtgcacgca gtggggacgg cctgagctct 60
ggctctggca cgggcagcct tacctggttg gaggtcaagg ccacgatgcg gtccaggtct 120
<210> 259
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 259
ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 60
caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 120
<210> 260
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 260
tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 60
attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 120
<210> 261
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 261
aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 60
caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 120
<210> 262
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 262
tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 60
aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 120
<210> 263
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 263
aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 60
tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 120
<210> 264
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 264
ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 60
tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 120
<210> 265
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 265
gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 60
gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 120
<210> 266
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 266
aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 60
gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 120
<210> 267
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 267
aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 60
taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 120
<210> 268
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 268
gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 60
aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 120
<210> 269
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 269
gcctatgccc ctccccctgc cgtccccggc catcctgccc cccagagtgc tgaggtgtgg 60
ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 120
<210> 270
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 270
taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 60
ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 120
<210> 271
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 271
gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 60
tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 120
<210> 272
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 272
tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 60
ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 120
<210> 273
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 273
gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 60
tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 120
<210> 274
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 274
agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 60
gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 120
<210> 275
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 275
gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 60
gcagtactcc ccgggtggcc aggacacccc cagctccagc tcctcagggg acgactccgt 120
<210> 276
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 276
catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 60
ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 120
<210> 277
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 277
ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 60
tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 120
<210> 278
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 278
tgaggggtcc ctagcagccc accctgctgc tggtgcacag ccactccccg gcatgagact 60
cagtgcagat ggagagacag ctacacagag ctttggtctg tgtgtgtgtg tgtgcgtgtg 120
<210> 279
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 279
atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 60
gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 120
<210> 280
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 280
aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 60
atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 120
<210> 281
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 281
tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 60
gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 120
<210> 282
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 282
ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 60
catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 120
<210> 283
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 283
agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 60
aataaagaca cctggttgct aacctggccc tgtgctttct gtctccagtt ctgggatagg 120
<210> 284
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 284
gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 60
acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 120
<210> 285
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 285
tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 60
aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 120
<210> 286
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 286
acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 60
gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 120
<210> 287
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 287
gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 60
ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 120
<210> 288
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 288
tgtgtgtgtg tgtgcacatc cgcgtgtgcc tgtgtgcgtg cgcatcttgc ctccaggtgc 60
agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 120
<210> 289
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 289
ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 60
gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 120
<210> 290
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 290
ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 60
accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 120
<210> 291
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 291
accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 60
gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 120
<210> 292
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 292
ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 60
gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 120
<210> 293
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 293
catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 60
catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 120
<210> 294
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 294
gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 60
agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 120
<210> 295
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 295
caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 60
cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 120
<210> 296
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 296
tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 60
aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 120
<210> 297
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 297
gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 60
ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 120
<210> 298
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 298
agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 60
tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 120
<210> 299
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 299
agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 60
agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 120
<210> 300
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 300
ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 60
ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 120
<210> 301
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 301
atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 60
atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 120
<210> 302
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 302
tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 60
aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 120
<210> 303
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 303
ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 60
caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 120
<210> 304
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 304
cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 60
aaggggcttc tagaaggaag ttcttacctc attggttgtg agagtgagaa ttcgatccaa 120
<210> 305
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 305
caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 60
ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 120
<210> 306
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 306
atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 60
cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 120
<210> 307
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 307
caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 60
gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 120
<210> 308
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 308
actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 60
cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 120
<210> 309
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 309
cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 60
cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 120
<210> 310
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 310
agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 60
gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 120
<210> 311
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 311
aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 60
ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 120
<210> 312
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 312
ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 60
gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 120
<210> 313
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 313
tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 60
taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 120
<210> 314
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 314
aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 60
ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 120
<210> 315
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 315
gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 60
tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 120
<210> 316
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 316
atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 60
gtttattttg ttggcaagga tcataaatta ttattatttt ctttttgaga tgaagtcttg 120
<210> 317
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 317
tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 60
aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 120
<210> 318
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 318
gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 60
aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 120
<210> 319
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 319
ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 60
acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 120
<210> 320
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 320
ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 60
ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 120
<210> 321
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 321
cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 60
ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 120
<210> 322
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 322
gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 60
ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 120
<210> 323
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 323
agttgccgac ccctgttctg aagctatgaa aagctttcta aattgggaac ttgtgggtct 60
ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 120
<210> 324
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 324
cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 60
gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 120
<210> 325
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 325
gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 60
aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 120
<210> 326
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 326
agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 60
gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 120
<210> 327
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 327
ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 60
actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 120
<210> 328
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 328
ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 60
ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 120
<210> 329
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 329
aatcttcccc aatgccaaat cactccagcc caagtgggat aaaaccattt ttcagcctct 60
caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 120
<210> 330
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 330
atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 60
agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 120
<210> 331
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 331
acaggcatga gccactgcgc ctgaccaact tttcccagtt tctcaatgaa gccataaact 60
ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 120
<210> 332
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 332
tccaagtatt cctgaaagaa gggaagagag acgttttatt tcatcttggg tcaggataac 60
aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 120
<210> 333
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 333
tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 60
ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 120
<210> 334
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 334
tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 60
gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 120
<210> 335
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 335
caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 60
gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 120
<210> 336
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 336
agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 60
agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 120
<210> 337
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 337
cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 60
cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 120
<210> 338
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 338
ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 60
caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 120
<210> 339
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 339
gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 60
ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 120
<210> 340
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 340
gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 60
gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 120
<210> 341
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 341
tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 60
tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 120
<210> 342
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 342
tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 60
catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 120
<210> 343
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 343
atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 60
agaacacatc tatttacata ttgtctgtgg ctactttgag gctacagtgg cgaagttgca 120
<210> 344
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 344
gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 60
tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 120
<210> 345
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 345
tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 60
aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 120
<210> 346
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 346
ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 60
tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 120
<210> 347
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 347
aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 60
agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 120
<210> 348
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 348
agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 60
atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 120
<210> 349
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 349
ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 60
caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 120
<210> 350
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 350
aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 60
cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 120
<210> 351
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 351
ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 60
ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 120
<210> 352
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 352
cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 60
aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 120
<210> 353
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 353
ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 60
ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 120
<210> 354
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 354
aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 60
caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 120
<210> 355
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 355
gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 60
cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 120
<210> 356
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 356
catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 60
cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 120
<210> 357
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 357
tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 60
tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 120
<210> 358
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 358
ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 60
atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 120
<210> 359
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 359
ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 60
ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 120
<210> 360
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 360
tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 60
aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 120
<210> 361
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 361
agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 60
cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 120
<210> 362
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 362
ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 60
tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 120
<210> 363
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 363
aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 60
tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 120
<210> 364
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 364
accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 60
ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 120
<210> 365
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 365
cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 60
tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 120
<210> 366
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 366
tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 60
acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 120
<210> 367
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 367
ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 60
gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 120
<210> 368
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 368
tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 60
aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 120
<210> 369
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 369
cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 60
gattaccttg aatggcaacg ctcctccgcg acctgtgttg tccccgcgcc ccgcgtgggt 120
<210> 370
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 370
ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 60
ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 120
<210> 371
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 371
gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 60
agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 120
<210> 372
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 372
taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 60
ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 120
<210> 373
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 373
atggtgtgtg cgtgcacggt gtgtgcatgt gctttcagat agcttgaaag atttttcctc 60
ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 120
<210> 374
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 374
tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 60
cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 120
<210> 375
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 375
aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 60
agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 120
<210> 376
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 376
cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 60
caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 120
<210> 377
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 377
aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 60
agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 120
<210> 378
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 378
atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 60
gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 120
<210> 379
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 379
aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 60
aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 120
<210> 380
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 380
cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 60
ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 120
<210> 381
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 381
gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 60
ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 120
<210> 382
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 382
caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 60
ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 120
<210> 383
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 383
gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 60
agcacaggtg aaacggtgtg attcacagca aaagcaagaa aagtatgtgt gcagtgtgtg 120
<210> 384
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 384
agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 60
aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 120
<210> 385
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 385
ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 60
tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 120
<210> 386
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 386
ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 60
atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 120
<210> 387
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 387
aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 60
gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 120
<210> 388
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 388
ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 60
tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 120
<210> 389
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 389
ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 60
gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 120
<210> 390
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 390
tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 60
tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 120
<210> 391
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 391
ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 60
atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 120
<210> 392
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 392
ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 60
tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 120
<210> 393
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 393
caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 60
gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 120
<210> 394
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 394
tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 60
tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 120
<210> 395
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 395
ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 60
ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 120
<210> 396
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 396
acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 60
agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 120
<210> 397
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 397
acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 60
accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 120
<210> 398
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 398
agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 60
ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 120
<210> 399
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 399
cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 60
cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 120
<210> 400
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 400
aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 60
agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 120
<210> 401
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 401
ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 60
gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 120
<210> 402
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 402
aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 60
ttttcccggt ttctttcttt tttattttta tttttttttt ttttgagacg gagtctcgct 120
<210> 403
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 403
gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 60
atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 120
<210> 404
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 404
gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 60
tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 120
<210> 405
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 405
acaggcgaga gcataaatat tttagggaaa ataaatattt taacaaaagg tacatgtttt 60
tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 120
<210> 406
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 406
gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 60
gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 120
<210> 407
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 407
cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 60
cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 120
<210> 408
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 408
gatgctccag gtggcataac ggacctgagg ggaaatgcca aagggataca ttgagggtcc 60
agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 120
<210> 409
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 409
agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 60
gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 120
<210> 410
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 410
gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 60
ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 120
<210> 411
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 411
ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 60
tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 120
<210> 412
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 412
tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 60
caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 120
<210> 413
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 413
caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 60
ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 120
<210> 414
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 414
ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 60
ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 120
<210> 415
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 415
ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 60
agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 120
<210> 416
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 416
agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 60
tcttgcagca tttaaagcat ggtgtcttgc atgtagtgag tgcatggtga attaatttaa 120
<210> 417
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 417
tgcttacacc ctttaagtta gtaatttcac ttgtaacaat ctgtcctaag gaagcacgct 60
taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 120
<210> 418
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 418
taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 60
tacctaatat cacaaaatag ttcattaaat tataggactt tttctgttgt tttttggaga 120
<210> 419
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 419
tggaatctta tacagtcatg aaaaattatg tttcaaatat tcatgcttga aatatatcag 60
gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 120
<210> 420
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 420
gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 60
atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 120
<210> 421
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 421
atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 60
tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 120
<210> 422
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 422
tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 60
gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 120
<210> 423
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 423
gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 60
cgcatgcaca cacacgtacc ttgtagcctc caattctgtg gtcaggtttg aattctttgc 120
<210> 424
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 424
agggctagaa gcgcgacggc ggtagcagct aggcttggcc cccggcgtgg agcagacgcg 60
gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 120
<210> 425
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 425
gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 60
ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 120
<210> 426
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 426
ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 60
gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 120
<210> 427
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 427
gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 60
tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 120
<210> 428
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 428
tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 60
cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 120
<210> 429
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 429
cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 60
gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 120
<210> 430
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 430
gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 60
tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 120
<210> 431
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 431
tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 60
ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 120
<210> 432
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 432
ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 60
acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 120
<210> 433
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 433
acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 60
gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 120
<210> 434
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 434
gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 60
gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 120
<210> 435
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 435
gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 60
ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 120
<210> 436
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 436
ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 60
tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 120
<210> 437
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 437
tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 60
gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 120
<210> 438
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 438
gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 60
ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 120
<210> 439
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 439
ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 60
ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 120
<210> 440
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 440
ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 60
ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 120
<210> 441
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 441
ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 60
tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 120
<210> 442
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 442
tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 60
ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 120
<210> 443
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 443
ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 60
gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 120
<210> 444
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 444
gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 60
ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 120
<210> 445
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 445
ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 60
ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 120
<210> 446
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 446
ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 60
gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 120
<210> 447
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 447
gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 60
cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 120
<210> 448
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 448
cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 60
ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 120
<210> 449
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 449
ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 60
ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 120
<210> 450
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 450
ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 60
catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 120
<210> 451
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 451
catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 60
tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 120
<210> 452
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 452
tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 60
tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 120
<210> 453
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 453
tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 60
ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 120
<210> 454
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 454
ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 60
tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 120
<210> 455
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 455
tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 60
gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 120
<210> 456
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 456
gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 60
gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 120
<210> 457
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 457
gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 60
aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 120
<210> 458
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 458
aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 60
cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 120
<210> 459
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 459
cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 60
agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 120
<210> 460
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 460
agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 60
cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 120
<210> 461
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 461
cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 60
gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 120
<210> 462
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 462
gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 60
aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 120
<210> 463
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 463
aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 60
gttcttgcag gaaagtgctg tcttgttcta tcaggccttg gctggatttt ttgtttgttt 120
<210> 464
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 464
aactgcaagt ggatggcgag gcggtgtctg tctttgccta gaaagggagg atcctggtgg 60
cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 120
<210> 465
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 465
cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 60
tccagggtgg gaagggaccc cagaggcatg attttctgtt ggttttttgc tgatgtaact 120
<210> 466
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 466
tttaagcaga ttttagatgt gcagagaaaa gcataaggaa cagaatacca ctgatgcttc 60
tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 120
<210> 467
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 467
tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 60
gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 120
<210> 468
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 468
gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 60
tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 120
<210> 469
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 469
tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 60
aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 120
<210> 470
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 470
aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 60
agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 120
<210> 471
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 471
agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 60
ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 120
<210> 472
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 472
ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 60
aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 120
<210> 473
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 473
aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 60
ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 120
<210> 474
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 474
ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 60
ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 120
<210> 475
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 475
ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 60
tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 120
<210> 476
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 476
tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 60
gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 120
<210> 477
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 477
gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 60
acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 120
<210> 478
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 478
acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 60
actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 120
<210> 479
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 479
actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 60
gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 120
<210> 480
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 480
gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 60
ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 120
<210> 481
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 481
ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 60
ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 120
<210> 482
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 482
ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 60
ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 120
<210> 483
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 483
ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 60
ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 120
<210> 484
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 484
ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 60
aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 120
<210> 485
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 485
aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 60
gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 120
<210> 486
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 486
gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 60
aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 120
<210> 487
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 487
aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 60
agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 120
<210> 488
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 488
agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 60
acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 120
<210> 489
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 489
acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 60
cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 120
<210> 490
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 490
cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 60
cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 120
<210> 491
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 491
cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 60
tggtgggcag gtgctcgaat ggatttttta caggaaatgg tggatgccgg gtgcggtggc 120
<210> 492
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 492
gcatcagctt gaggcatttg ggtgtgctgt cccacatctg accccccagg cagcagcctg 60
tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 120
<210> 493
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 493
tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 60
ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 120
<210> 494
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 494
ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 60
aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 120
<210> 495
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 495
aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 60
ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 120
<210> 496
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 496
ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 60
tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 120
<210> 497
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 497
tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 60
gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 120
<210> 498
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 498
gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 60
aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 120
<210> 499
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 499
aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 60
aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 120
<210> 500
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 500
aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 60
ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 120
<210> 501
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 501
ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 60
tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 120
<210> 502
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 502
tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 60
ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 120
<210> 503
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 503
ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 60
gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 120
<210> 504
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 504
gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 60
accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 120
<210> 505
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 505
accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 60
gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 120
<210> 506
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 506
gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 60
gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 120
<210> 507
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 507
gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 60
ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 120
<210> 508
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 508
ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 60
acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 120
<210> 509
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 509
acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 60
agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 120
<210> 510
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 510
agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 60
gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 120
<210> 511
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 511
gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 60
gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 120
<210> 512
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 512
gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 60
tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 120
<210> 513
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 513
tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 60
gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 120
<210> 514
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 514
gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 60
ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 120
<210> 515
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 515
ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 60
gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 120
<210> 516
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 516
gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 60
cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 120
<210> 517
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 517
cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 60
agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 120
<210> 518
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 518
agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 60
tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 120
<210> 519
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 519
tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 60
ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 120
<210> 520
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 520
ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 60
ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 120
<210> 521
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 521
ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 60
taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 120
<210> 522
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 522
taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 60
agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 120
<210> 523
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 523
agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 60
cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 120
<210> 524
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 524
cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 60
tcctggctga atggtcttag gctacatctc ctagagttgc tcagtgggag attgtcctgc 120
<210> 525
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 525
tgggtggctt gctgcccaca cctgccccag cctccacatt tgaagccttt tgtcagtttc 60
acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 120
<210> 526
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 526
acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 60
agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 120
<210> 527
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 527
agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 60
ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 120
<210> 528
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 528
ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 60
agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 120
<210> 529
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 529
agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 60
ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 120
<210> 530
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 530
ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 60
ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 120
<210> 531
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 531
ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 60
ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 120
<210> 532
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 532
ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 60
tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 120
<210> 533
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 533
tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 60
gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 120
<210> 534
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 534
gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 60
ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 120
<210> 535
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 535
ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 60
gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 120
<210> 536
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 536
gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 60
tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 120
<210> 537
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 537
tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 60
tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 120
<210> 538
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 538
tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 60
ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 120
<210> 539
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 539
ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 60
gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 120
<210> 540
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 540
gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 60
gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 120
<210> 541
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 541
gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 60
atgagcagga gaggtgaaca atgcagtgcg ctaatgagat acaagtgtag agtcaacaac 120
<210> 542
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 542
tgcagttaaa taaatacggt ttgtccgtaa gattggaggc tgcagttaaa taaatacggt 60
ttgtccgtaa gattggaggc tgcagttaaa taaatacggt ttgtccgtaa gattggaggc 120
<210> 543
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 543
aataaatacg gtttgtccgt aagattggaa ggtggaacgg tccttaaatt aaagtgcatt 60
tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 120
<210> 544
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 544
tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 60
caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 120
<210> 545
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 545
caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 60
gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 120
<210> 546
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 546
gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 60
tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 120
<210> 547
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 547
tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 60
cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 120
<210> 548
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 548
cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 60
cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 120
<210> 549
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 549
cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 60
ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 120
<210> 550
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 550
ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 60
atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 120
<210> 551
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 551
atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 60
tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 120
<210> 552
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 552
tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 60
actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 120
<210> 553
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 553
actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 60
ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 120
<210> 554
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 554
ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 60
ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 120
<210> 555
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 555
ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 60
aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 120
<210> 556
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 556
aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 60
gaggtaaatc ccttttattc aagaagtgag ggaaaggaaa aaatacaaag ttaagtccct 120
<210> 557
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 557
gacaaggcca tagcagagaa ggacctacaa gtttttgcta gactaaataa ttggcaaccg 60
ttttttagct taattgagaa aaggagagaa tgagcaaata ctgctaggca tgaagaaagg 120
<210> 558
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 558
ccacccttag ttttaatcaa aagttatatt atcaaaaatt aaaagacttg tctagggtaa 60
ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 120
<210> 559
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 559
ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 60
gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 120
<210> 560
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 560
gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 60
cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 120
<210> 561
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 561
cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 60
acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 120
<210> 562
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 562
acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 60
gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 120
<210> 563
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 563
gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 60
ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 120
<210> 564
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 564
ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 60
tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 120
<210> 565
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 565
tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 60
gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 120
<210> 566
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 566
gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 60
aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 120
<210> 567
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 567
aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 60
cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 120
<210> 568
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 568
cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 60
gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 120
<210> 569
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 569
gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 60
gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 120
<210> 570
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 570
gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 60
tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 120
<210> 571
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 571
tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 60
gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 120
<210> 572
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 572
gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 60
agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 120
<210> 573
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 573
agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 60
gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 120
<210> 574
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 574
gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 60
tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 120
<210> 575
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 575
tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 60
gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 120
<210> 576
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 576
gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 60
cagggctgct gccttgctgg ttacagccac cgttctaggt atattaaaga tctaaactta 120
<210> 577
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 577
gcaaactatc acctgtgggc cagacttggc cactgcctgt ttgtgtaaag ttttatcgtc 60
acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 120
<210> 578
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 578
acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 60
gagtagtgtg acagaggctg tgtggccttc aaatcctaac acatggccgg gcgcagtggc 120
<210> 579
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 579
aacagccccc tgatgaggca gcactggctc cgctcagcct gcagtgggta atgaagcgtc 60
accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 120
<210> 580
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 580
accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 60
cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 120
<210> 581
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 581
cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 60
acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 120
<210> 582
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 582
acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 60
gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 120
<210> 583
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 583
gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 60
tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 120
<210> 584
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 584
tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 60
atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 120
<210> 585
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 585
atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 60
tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 120
<210> 586
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 586
tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 60
cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 120
<210> 587
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 587
cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 60
tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 120
<210> 588
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 588
tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 60
cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 120
<210> 589
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 589
cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 60
aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 120
<210> 590
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 590
aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 60
ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 120
<210> 591
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 591
ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 60
tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 120
<210> 592
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 592
tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 60
gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 120
<210> 593
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 593
gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 60
tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 120
<210> 594
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 594
tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 60
gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 120
<210> 595
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 595
gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 60
ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 120
<210> 596
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 596
ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 60
gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 120
<210> 597
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 597
gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 60
catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 120
<210> 598
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 598
catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 60
catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 120
<210> 599
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 599
catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 60
cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 120
<210> 600
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 600
cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 60
cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 120
<210> 601
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 601
cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 60
acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 120
<210> 602
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 602
acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 60
gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 120
<210> 603
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 603
gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 60
ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 120
<210> 604
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 604
ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 60
gggggcaggt ggcccctgca tgtaacttat gttagaaatg cacaatgctt gttctctggt 120
<210> 605
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 605
ctgccactgg gctgcggggg cagctgcagg gacacacacc tgttgggtgc ctgctggttc 60
cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 120
<210> 606
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 606
cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 60
gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 120
<210> 607
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 607
gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 60
ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 120
<210> 608
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 608
ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 60
aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 120
<210> 609
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 609
aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 60
taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 120
<210> 610
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 610
taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 60
agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 120
<210> 611
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 611
agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 60
gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 120
<210> 612
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 612
gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 60
cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 120
<210> 613
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 613
cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 60
tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 120
<210> 614
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 614
tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 60
gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 120
<210> 615
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 615
gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 60
ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 120
<210> 616
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 616
ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 60
gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 120
<210> 617
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 617
gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 60
ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 120
<210> 618
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 618
ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 60
tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 120
<210> 619
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 619
tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 60
ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 120
<210> 620
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 620
ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 60
gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 120
<210> 621
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 621
gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 60
tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 120
<210> 622
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 622
tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 60
ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 120
<210> 623
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 623
ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 60
gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 120
<210> 624
<211> 120
<212> DNA
<213> Artificial
<220>
<221> misc_binding
<223>probe
<400> 624
gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 60
ccagttccct gcggatctga tgtggaggaa acagctgttt cctgggcttt gcatccggcc 120
Claims (9)
1. the probe combinations for detecting human gene FGFR2/3 Gene Fusion, the probe combinations include following probes:
For the multicore glycosides of initiation site 1729490 on No. 4 chromosomes where people's TACC3 gene to termination site 1729609
Acid sequence or its complementary series, probe sequence are SEQ ID No:501,
For the multicore glycosides of initiation site 1729550 on No. 4 chromosomes where people's TACC3 gene to termination site 1729669
Acid sequence or its complementary series, probe sequence are SEQ ID No:502,
For the multicore glycosides of initiation site 1729610 on No. 4 chromosomes where people's TACC3 gene to termination site 1729729
Acid sequence or its complementary series, probe sequence are SEQ ID No:503,
For the multicore glycosides of initiation site 1729670 on No. 4 chromosomes where people's TACC3 gene to termination site 1729789
Acid sequence or its complementary series, probe sequence are SEQ ID No:504,
For the multicore glycosides of initiation site 1729730 on No. 4 chromosomes where people's TACC3 gene to termination site 1729849
Acid sequence or its complementary series, probe sequence are SEQ ID No:505,
For the multicore glycosides of initiation site 1729790 on No. 4 chromosomes where people's TACC3 gene to termination site 1729909
Acid sequence or its complementary series, probe sequence are SEQ ID No:506,
For the multicore glycosides of initiation site 1808662 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808781
Acid sequence or its complementary series, probe sequence are SEQ ID No:72,
For the multicore glycosides of initiation site 1808782 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808901
Acid sequence or its complementary series, probe sequence are SEQ ID No:79,
For the multicore glycosides of initiation site 1808722 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808841
Acid sequence or its complementary series, probe sequence are SEQ ID No:96,
For the multicore glycosides of initiation site 1808692 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808811
Acid sequence or its complementary series, probe sequence are SEQ ID No:269,
For the multicore glycosides of initiation site 1808752 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808871
Acid sequence or its complementary series, probe sequence are SEQ ID No:292,
For the multicore glycosides of initiation site 1808812 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808931
Acid sequence or its complementary series, probe sequence are SEQ ID No:275,
For the more of initiation site 123241115 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241234
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:111,
For the more of initiation site 123241175 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241294
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:158,
For the more of initiation site 123241055 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241174
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:165,
For the more of initiation site 123240995 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241114
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:187,
For the more of initiation site 123241295 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241414
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:193,
For the more of initiation site 123241235 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241354
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:212,
For the more of initiation site 123240965 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241084
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:295,
For the more of initiation site 123241145 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241264
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:306,
For the more of initiation site 123241205 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241324
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:352,
For the more of initiation site 123241085 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241204
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:358,
For the more of initiation site 123241025 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241144
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:380,
For the more of initiation site 123241265 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241384
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:402,
For the more of initiation site 123240935 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241054
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:100,
For the more of initiation site 123242135 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242254
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:106,
For the more of initiation site 123243095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243214
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:109,
For the more of initiation site 123240755 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240874
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:113,
For the more of initiation site 123240815 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240934
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:114,
For the more of initiation site 123240275 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240394
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:115,
For the more of initiation site 123242195 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242314
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:121,
For the more of initiation site 123240515 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240634
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:123,
For the more of initiation site 123241655 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241774
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:125,
For the more of initiation site 123240335 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240454
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:128,
For the more of initiation site 123240635 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240754
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:129,
For the more of initiation site 123239735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239854
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:131,
For the more of initiation site 123240695 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240814
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:133,
For the more of initiation site 123241595 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241714
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:137,
For the more of initiation site 123239495 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239614
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:138,
For the more of initiation site 123239915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240034
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:139,
For the more of initiation site 123242735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242854
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:143,
For the more of initiation site 123242975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243094
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:148,
For the more of initiation site 123240455 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240574
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:149,
For the more of initiation site 123240095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240214
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:150,
For the more of initiation site 123241895 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242014
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:151,
For the more of initiation site 123240215 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240334
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:153,
For the more of initiation site 123242675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242794
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:156,
For the more of initiation site 123239975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240094
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:157,
For the more of initiation site 123240575 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240694
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:160,
For the more of initiation site 123243035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243154
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:163,
For the more of initiation site 123239855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239974
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:164,
For the more of initiation site 123240155 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240274
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:167,
For the more of initiation site 123239795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239914
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:170,
For the more of initiation site 123241775 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241894
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:171,
For the more of initiation site 123242915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243034
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:172,
For the more of initiation site 123242615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242734
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:175,
For the more of initiation site 123241955 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242074
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:182,
For the more of initiation site 123240875 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240994
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:183,
For the more of initiation site 123239555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239674
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:186,
For the more of initiation site 123242255 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242374
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:188,
For the more of initiation site 123240395 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240514
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:195,
For the more of initiation site 123239615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239734
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:197,
For the more of initiation site 123240035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240154
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:199,
For the more of initiation site 123242075 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242194
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:201,
For the more of initiation site 123241835 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241954
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:202,
For the more of initiation site 123241715 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241834
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:207,
For the more of initiation site 123242015 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242134
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:208,
For the more of initiation site 123242855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242974
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:209,
For the more of initiation site 123242555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242674
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:215,
For the more of initiation site 123239675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239794
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:216,
For the more of initiation site 123242795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242914
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:217,
For the more of initiation site 123242165 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242284
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:301,
For the more of initiation site 123243125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243244
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:304,
For the more of initiation site 123240785 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240904
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:308,
For the more of initiation site 123240845 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240964
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:309,
For the more of initiation site 123240305 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240424
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:310,
For the more of initiation site 123242225 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242344
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:316,
For the more of initiation site 123241685 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241804
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:319,
For the more of initiation site 123240365 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240484
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:322,
For the more of initiation site 123240665 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240784
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:323,
For the more of initiation site 123239765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239884
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:325,
For the more of initiation site 123240725 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240844
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:327,
For the more of initiation site 123241625 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241744
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:331,
For the more of initiation site 123239525 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239644
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:332,
For the more of initiation site 123239945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240064
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:333,
For the more of initiation site 123242765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242884
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:337,
For the more of initiation site 123243005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243124
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:342,
For the more of initiation site 123240485 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240604
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:343,
For the more of initiation site 123240125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240244
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:344,
For the more of initiation site 123241925 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242044
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:345,
For the more of initiation site 123240245 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240364
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:347,
For the more of initiation site 123242705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242824
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:350,
For the more of initiation site 123240005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240124
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:351,
For the more of initiation site 123243065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243184
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:356,
For the more of initiation site 123239885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240004
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:357,
For the more of initiation site 123240185 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240304
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:360,
For the more of initiation site 123239825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239944
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:363,
For the more of initiation site 123241805 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241924
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:364,
For the more of initiation site 123242945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243064
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:365,
For the more of initiation site 123242645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242764
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:368,
For the more of initiation site 123241985 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242104
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:375,
For the more of initiation site 123240905 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241024
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:376,
For the more of initiation site 123239585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239704
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:379,
For the more of initiation site 123240425 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240544
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:386,
For the more of initiation site 123239645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239764
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:387,
For the more of initiation site 123240065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240184
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:389,
For the more of initiation site 123242105 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242224
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:391,
For the more of initiation site 123241865 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241984
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:392,
For the more of initiation site 123241745 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241864
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:397,
For the more of initiation site 123242045 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242164
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:398,
For the more of initiation site 123242885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243004
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:399,
For the more of initiation site 123242585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242704
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:405,
For the more of initiation site 123239705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239824
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:406,
For the more of initiation site 123242825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242944
Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:407,
For the multicore glycosides of initiation site 1809043 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809162
Acid sequence or its complementary series, probe sequence are SEQ ID No:74,
For the multicore glycosides of initiation site 1809163 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809282
Acid sequence or its complementary series, probe sequence are SEQ ID No:78,
For the multicore glycosides of initiation site 1808983 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809102
Acid sequence or its complementary series, probe sequence are SEQ ID No:82,
For the multicore glycosides of initiation site 1809283 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809402
Acid sequence or its complementary series, probe sequence are SEQ ID No:86,
For the multicore glycosides of initiation site 1809223 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809342
Acid sequence or its complementary series, probe sequence are SEQ ID No:91,
For the multicore glycosides of initiation site 1809103 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809222
Acid sequence or its complementary series, probe sequence are SEQ ID No:92,
For the multicore glycosides of initiation site 1809343 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809462
Acid sequence or its complementary series, probe sequence are SEQ ID No:97,
For the multicore glycosides of initiation site 1809193 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809312
Acid sequence or its complementary series, probe sequence are SEQ ID No:274,
For the multicore glycosides of initiation site 1809013 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809132
Acid sequence or its complementary series, probe sequence are SEQ ID No:278,
For the multicore glycosides of initiation site 1809313 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809432
Acid sequence or its complementary series, probe sequence are SEQ ID No:282,
For the multicore glycosides of initiation site 1809253 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809372
Acid sequence or its complementary series, probe sequence are SEQ ID No:287,
For the multicore glycosides of initiation site 1809133 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809252
Acid sequence or its complementary series, probe sequence are SEQ ID No:288.
2. probe combinations described in claim 1, wherein human gene FGFR2/3 Gene Fusion include FGFR2-COL14A1,
FGFR3-TACC3 and FGFR3-BAIAP2L1.
3. probe combinations of any of claims 1 or 2 are in preparation for detecting human gene FGFR2/3 gene in subject's sample
Purposes in the kit of fusion.
4. purposes as claimed in claim 3, wherein the sample includes tissue biopsy sample, FFPE sample and cfDNA sample,
The subject is normal healthy subjects, suspects or be diagnosed as the subject with disease.
5. purposes as claimed in claim 3, wherein the sample includes DNA sample and liquid biopsy blood plasma.
6. purposes as claimed in claim 4, wherein the disease is cancer.
7. the kit for detecting human gene FGFR2/3 Gene Fusion comprising capture reagent, the capture reagent include
Probe combinations of any of claims 1 or 2.
8. kit as claimed in claim 7 further includes one of following or a variety of: 1) build library reagent, 2) except probe with
Outer capture reagent, 3) solid phase carrier and/or 4) high-flux sequence reagent.
9. kit according to any one of claims 8, wherein the library reagent of building includes archaeal dna polymerase, ligase, buffer and/or
Quality-control product;The capture reagent in addition to probe includes sealer, captures buffer and/or cleaning solution;The solid phase carrier
Including magnetic bead.
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CN113249444A (en) * | 2021-05-17 | 2021-08-13 | 苏州吉因加生物医学工程有限公司 | DNA probe library for hybridization with FGFR1, 2 or 3 gene and application |
CN113249490A (en) * | 2021-07-06 | 2021-08-13 | 迈杰转化医学研究(苏州)有限公司 | Probe library for detecting FGFR family gene inhibitor sensitivity and drug resistance variation and application thereof |
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CN113249444B (en) * | 2021-05-17 | 2023-10-13 | 苏州吉因加生物医学工程有限公司 | DNA probe library for hybridization with FGFR1, 2 or 3 genes and application thereof |
CN113249490A (en) * | 2021-07-06 | 2021-08-13 | 迈杰转化医学研究(苏州)有限公司 | Probe library for detecting FGFR family gene inhibitor sensitivity and drug resistance variation and application thereof |
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