CN110218797A - FGFR Gene Fusion probe, detection method and kit - Google Patents

Abstract

The present invention relates to FGFR Gene Fusion probe, detection method and kits.The present invention provides the probe combinations of detection people FGFR Gene Fusion comprising following probes: FGFR2(NM_001144917): whole exons and 1,15, No. 17 probe coverings for including subregion；FGFR3(NM_000142): whole exons and the covering of No. 17,18 intrones and the probe of 3 ' UTR regions；FGFR3 chaperone TACC3(NM_006342) the 11st, No. 14 probe covering for including subregion.The present invention also mentions the kit of detection FGFR Gene Fusion, especially the disposable detection kit of FGFR Gene Fusion and a kind of for analyzing the automation equipment of human gene FGFR Gene Fusion.The present invention is suitable for the detection of extensive sample.

Description

FGFR Gene Fusion probe, detection method and kit

Technical field

The invention belongs to biology technique of gene detection and technical field of biological information, and in particular to Gene Fusion detection Capture probe design, high-flux sequence (NGS) experiment and data analysing method and kit.

Background technique

Fibroblast growth factor acceptor (FGFR) family mainly include 4 seed types receptor FGFR1, FGFR2, FGFR3 and FGFR4 plays a significant role in angiogenesis, angiogenesis, wound healing and embryonic development.FGFR is cross-film Albumen has extracellular domain, tyrosine kinase domain in transmembrane domain and cytoplasm.FGF ligand is in conjunction with FGFR Signal transduction cascade of events in active cell.

FGFR gene mutation includes that Gene Fusion causes various diseases.Gene Fusion belongs to one kind of genetic mutation, refers to Since the reasons such as chromosomal structural aberration, including missing, repetition, inversion, transposition lead to different positions on two or more chromosomes The gene set joins end to end the mosaic gene of composition, and expression product is fusion protein.In Gene Fusion, the whole of two genes Or the sequence of a part mutually permeates a new gene.Gene Fusion is likely to be chromosome translocation, intercalary delection or dye Colour solid is inverted caused result.The driving gene that fusion is cancer occurs for certain kinases, and corresponding gene target inhibitor is also Through granted, including the inhibitor Gleevec for BCR-ABL fusion；Buddhist nun is replaced for the inhibitor gram azoles of ALK fusion (crizotinib), Ai Le replaces Buddhist nun (alectinib), Ceritinib (ceritinib), and Laura replaces Buddhist nun (lorlatinib)；Needle Buddhist nun (crizotinib) is replaced to the inhibitor gram azoles of ROS1 fusion；For the inhibitor Larotrectinib of NTRK fusion.Tradition The method for detecting these Gene Fusions is usually FISH and IHC, but these flux are lower, and needs individually to set for individual gene Kit is counted, and the chaperone merged is generally difficult to detect, therefore also restricted in clinical practice.

High throughput sequencing technologies are also known as next-generation sequencing technologies (Next Generation Sequencing, abbreviation NGS), once sequencing can be carried out to the hundreds of thousands in cellular genome to up to ten million molecules parallel, therefore in genome It is widely used in research.A large amount of sequences can be quickly sequenced in high-flux sequence, can be used in Different Individual Such as the detection in Gene Mutation of tumor patient, provide for individualized treatment beneficial to hints and tips.NGS provides a kind of detection fusion High throughput, the method for high-precision and hypersensitivity.

Summary of the invention

The present invention provides a kind of highly sensitive and is specifically used for detection FGFR1, FGFR2 and FGFR3 Gene Fusion (especially Human gene FGFR2/3 Gene Fusion) variation capture probe, kit and data analysing method.Detection of the invention Kit can be used for various samples, such as tumor tissue biopsy sample, FFPE sample and liquid biopsy blood plasma, and cfDNA etc. is no The DNA sample of same type.Method of the invention and the sensibility and specificity of kit detection are high, reproducible, press down for FGFR The clinical research of preparation provides a kind of biomarker screening method.

In some embodiments, the present invention is provided to detect human gene FGFR1/2/3 Gene Fusion (especially people Genoid FGFR2/3 Gene Fusion) probe combinations comprising FGFR2-COL14A1, FGFR3-TACC3, FGFR3- BAIAP2L1 merges the probe of position, and the probe combinations include following probes selected from subordinate list one:

For the multicore glycosides of initiation site 1729490 on No. 4 chromosomes where people's TACC3 gene to termination site 1729609 Acid sequence or its complementary series, probe sequence are SEQ ID No:501,

For the multicore glycosides of initiation site 1729550 on No. 4 chromosomes where people's TACC3 gene to termination site 1729669 Acid sequence or its complementary series, probe sequence are SEQ ID No:502,

For the multicore glycosides of initiation site 1729610 on No. 4 chromosomes where people's TACC3 gene to termination site 1729729 Acid sequence or its complementary series, probe sequence are SEQ ID No:503,

For the multicore glycosides of initiation site 1729670 on No. 4 chromosomes where people's TACC3 gene to termination site 1729789 Acid sequence or its complementary series, probe sequence are SEQ ID No:504,

For the multicore glycosides of initiation site 1729730 on No. 4 chromosomes where people's TACC3 gene to termination site 1729849 Acid sequence or its complementary series, probe sequence are SEQ ID No:505,

For the multicore glycosides of initiation site 1729790 on No. 4 chromosomes where people's TACC3 gene to termination site 1729909 Acid sequence or its complementary series, probe sequence are SEQ ID No:506,

For the multicore glycosides of initiation site 1808662 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808781 Acid sequence or its complementary series, probe sequence are SEQ ID No:72,

For the multicore glycosides of initiation site 1808782 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808901 Acid sequence or its complementary series, probe sequence are SEQ ID No:79,

For the multicore glycosides of initiation site 1808722 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808841 Acid sequence or its complementary series, probe sequence are SEQ ID No:96,

For the multicore glycosides of initiation site 1808692 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808811 Acid sequence or its complementary series, probe sequence are SEQ ID No:269,

For the multicore glycosides of initiation site 1808752 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808871 Acid sequence or its complementary series, probe sequence are SEQ ID No:292,

For the multicore glycosides of initiation site 1808812 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808931 Acid sequence or its complementary series, probe sequence are SEQ ID No:275,

For the more of initiation site 123241115 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241234 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:111,

For the more of initiation site 123241175 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241294 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:158,

For the more of initiation site 123241055 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241174 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:165,

For the more of initiation site 123240995 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241114 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:187,

For the more of initiation site 123241295 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241414 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:193,

For the more of initiation site 123241235 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241354 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:212,

For the more of initiation site 123240965 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241084 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:295,

For the more of initiation site 123241145 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241264 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:306,

For the more of initiation site 123241205 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241324 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:352,

For the more of initiation site 123241085 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241204 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:358,

For the more of initiation site 123241025 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241144 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:380,

For the more of initiation site 123241265 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241384 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:402,

For the more of initiation site 123240935 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241054 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:100,

For the more of initiation site 123242135 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242254 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:106,

For the more of initiation site 123243095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243214 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:109,

For the more of initiation site 123240755 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240874 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:113,

For the more of initiation site 123240815 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240934 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:114,

For the more of initiation site 123240275 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240394 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:115,

For the more of initiation site 123242195 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242314 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:121,

For the more of initiation site 123240515 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240634 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:123,

For the more of initiation site 123241655 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241774 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:125,

For the more of initiation site 123240335 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240454 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:128,

For the more of initiation site 123240635 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240754 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:129,

For the more of initiation site 123239735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239854 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:131,

For the more of initiation site 123240695 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240814 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:133,

For the more of initiation site 123241595 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241714 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:137,

For the more of initiation site 123239495 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239614 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:138,

For the more of initiation site 123239915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240034 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:139,

For the more of initiation site 123242735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242854 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:143,

For the more of initiation site 123242975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243094 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:148,

For the more of initiation site 123240455 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240574 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:149,

For the more of initiation site 123240095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240214 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:150,

For the more of initiation site 123241895 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242014 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:151,

For the more of initiation site 123240215 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240334 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:153,

For the more of initiation site 123242675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242794 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:156,

For the more of initiation site 123239975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240094 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:157,

For the more of initiation site 123240575 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240694 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:160,

For the more of initiation site 123243035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243154 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:163,

For the more of initiation site 123239855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239974 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:164,

For the more of initiation site 123240155 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240274 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:167,

For the more of initiation site 123239795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239914 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:170,

For the more of initiation site 123241775 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241894 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:171,

For the more of initiation site 123242915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243034 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:172,

For the more of initiation site 123242615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242734 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:175,

For the more of initiation site 123241955 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242074 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:182,

For the more of initiation site 123240875 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240994 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:183,

For the more of initiation site 123239555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239674 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:186,

For the more of initiation site 123242255 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242374 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:188,

For the more of initiation site 123240395 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240514 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:195,

For the more of initiation site 123239615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239734 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:197,

For the more of initiation site 123240035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240154 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:199,

For the more of initiation site 123242075 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242194 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:201,

For the more of initiation site 123241835 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241954 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:202,

For the more of initiation site 123241715 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241834 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:207,

For the more of initiation site 123242015 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242134 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:208,

For the more of initiation site 123242855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242974 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:209,

For the more of initiation site 123242555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242674 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:215,

For the more of initiation site 123239675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239794 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:216,

For the more of initiation site 123242795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242914 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:217,

For the more of initiation site 123242165 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242284 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:301,

For the more of initiation site 123243125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243244 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:304,

For the more of initiation site 123240785 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240904 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:308,

For the more of initiation site 123240845 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240964 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:309,

For the more of initiation site 123240305 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240424 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:310,

For the more of initiation site 123242225 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242344 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:316,

For the more of initiation site 123241685 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241804 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:319,

For the more of initiation site 123240365 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240484 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:322,

For the more of initiation site 123240665 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240784 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:323,

For the more of initiation site 123239765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239884 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:325,

For the more of initiation site 123240725 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240844 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:327,

For the more of initiation site 123241625 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241744 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:331,

For the more of initiation site 123239525 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239644 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:332,

For the more of initiation site 123239945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240064 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:333,

For the more of initiation site 123242765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242884 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:337,

For the more of initiation site 123243005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243124 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:342,

For the more of initiation site 123240485 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240604 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:343,

For the more of initiation site 123240125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240244 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:344,

For the more of initiation site 123241925 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242044 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:345,

For the more of initiation site 123240245 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240364 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:347,

For the more of initiation site 123242705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242824 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:350,

For the more of initiation site 123240005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240124 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:351,

For the more of initiation site 123243065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243184 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:356,

For the more of initiation site 123239885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240004 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:357,

For the more of initiation site 123240185 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240304 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:360,

For the more of initiation site 123239825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239944 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:363,

For the more of initiation site 123241805 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241924 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:364,

For the more of initiation site 123242945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243064 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:365,

For the more of initiation site 123242645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242764 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:368,

For the more of initiation site 123241985 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242104 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:375,

For the more of initiation site 123240905 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241024 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:376,

For the more of initiation site 123239585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239704 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:379,

For the more of initiation site 123240425 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240544 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:386,

For the more of initiation site 123239645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239764 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:387,

For the more of initiation site 123240065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240184 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:389,

For the more of initiation site 123242105 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242224 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:391,

For the more of initiation site 123241865 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241984 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:392,

For the more of initiation site 123241745 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241864 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:397,

For the more of initiation site 123242045 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242164 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:398,

For the more of initiation site 123242885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243004 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:399,

For the more of initiation site 123242585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242704 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:405,

For the more of initiation site 123239705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239824 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:406,

For the more of initiation site 123242825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242944 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:407,

For the multicore glycosides of initiation site 1809043 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809162 Acid sequence or its complementary series, probe sequence are SEQ ID No:74,

For the multicore glycosides of initiation site 1809163 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809282 Acid sequence or its complementary series, probe sequence are SEQ ID No:78,

For the multicore glycosides of initiation site 1808983 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809102 Acid sequence or its complementary series, probe sequence are SEQ ID No:82,

For the multicore glycosides of initiation site 1809283 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809402 Acid sequence or its complementary series, probe sequence are SEQ ID No:86,

For the multicore glycosides of initiation site 1809223 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809342 Acid sequence or its complementary series, probe sequence are SEQ ID No:91,

For the multicore glycosides of initiation site 1809103 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809222 Acid sequence or its complementary series, probe sequence are SEQ ID No:92,

For the multicore glycosides of initiation site 1809343 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809462 Acid sequence or its complementary series, probe sequence are SEQ ID No:97,

For the multicore glycosides of initiation site 1809193 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809312 Acid sequence or its complementary series, probe sequence are SEQ ID No:274,

For the multicore glycosides of initiation site 1809013 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809132 Acid sequence or its complementary series, probe sequence are SEQ ID No:278,

For the multicore glycosides of initiation site 1809313 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809432 Acid sequence or its complementary series, probe sequence are SEQ ID No:282,

For the multicore glycosides of initiation site 1809253 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809372 Acid sequence or its complementary series, probe sequence are SEQ ID No:287,

For the multicore glycosides of initiation site 1809133 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809252 Acid sequence or its complementary series, probe sequence are SEQ ID No:288.

In some embodiments, it has been found that probe combinations of the invention can be wide with highly sensitive and specific detection General FGFR1/2/3(especially FGFR2/3) Gene Fusion, especially include such as FGFR2-COL14A1, FGFR3-TACC3 and FGFR3-BAIAP2L1 fusion.In some embodiments, it has been found that probe combinations of the invention can with highly sensitive and The extensive FGFR1/2/3(of specific detection especially FGFR2/3) Gene Fusion, especially include such as ZNF198-FGFR1, FGFR2-BICC1 and FGFR3-WHSC1.

In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene The probe combinations of fusion include the probe selected from following one or more fusions for FGFR1 selected from subordinate list one: BAG4-FGFR1、FGFR1OP-FGFR1、FN1-FGFR1、CEP110-FGFR1、TPR-FGFR1、BCR-FGFR1、CPSF6- FGFR1、ERLIN2-FGFR1、FGFR1-FGFR1、FGFR1-TACC1、HERV-K-FGFR1、ACPP-FGFR1、CUX1- FGFR1、FGFR1-ADAM18、FGFR1-ADGRA2、FGFR1-CSMD1、FGFR1-LETM2、FGFR1OP2-FGFR1、FGFR1- TTLL1、FOXO1-FGFR1、LRRFIP1-FGFR1、MYO18A-FGFR1、NUP98-FGFR1、RAB11FIP1-FGFR1、 RANBP2-FGFR1, RHOT1-FGFR1, SQSTM1-FGFR1, TRIM24-FGFR1 and/or ZNF198-FGFR1.

In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene The probe combinations of fusion include the probe selected from following one or more fusions for FGFR2 selected from subordinate list one: FGFR2-BICC1、FGFR2-KIAA1217、FGFR2-AHCYL1、FGFR2-CCDC6、FGFR2-NOL4、FGFR2-TACC2、 FGFR2-KIAA1967、FGFR2-NPM1、FGFR2-NRAP、FGFR2-POC1B、FGFR2-ARHGAP24、FGFR2-CASP7、 FGFR2-CIT、FGFR2-KIAA1598、FGFR2-LAMC1、FGFR2-MACF1、FGFR2-OFD1、FGFR2-PAWR、FGFR2- SHTN1、FGFR2-SPERT、FGFR2-TACC1、FGFR2-TACC3、FGFR2-WAC、AHCYL1-FGFR2、CCAR2-FGFR2、 CCDC46-FGFR2、CTNNA3-FGFR2、DNM1-FGFR2、FGFR2-ACLY、FGFR2-AFF1、FGFR2-AFF3、FGFR2- AFR、FGFR2-AP1M1、FGFR2-ATF1、FGFR2-ATF2、FGFR2-BFSP2、FGFR2-C10ORF118、FGFR2- C10orf68、FGFR2-CBX5、FGFR2-CCAR2、FGFR2-CCDC158、FGFR2-CCDC186、FGFR2-COL16A1、 FGFR2-CTNNA3、FGFR2-EIF4A2、FGFR2-ENPP2、FGFR2-ETV6、FGFR2-FAM76A、FGFR2-FILIP1、 FGFR2-FRK、FGFR2-GAB2、FGFR2-GOPC、FGFR2-KCNH7、FGFR2-KIF14、FGFR2-KLRAQ1、FGFR2- MGEA5、FGFR2-NCALD、FGFR2-NRBF2、FGFR2-PPAPDC1A、FGFR2-PPHLN1、FGFR2-RABGAP1L、 FGFR2-RABPGAP1L、FGFR2-RASAL2、FGFR2-RASSF4、FGFR2-ROCK1、FGFR2-SH3GLB、FGFR2- SHC2、FGFR2-SHROOM、FGFR2-SLMAP、FGFR2-SMN1、FGFR2-SOGA1、FGFR2-SORBS1、FGFR2- SPICE1、FGFR2-STRN4、FGFR2-TFEC、FGFR2-TNS1、FGFR2-TRA2B、FGFR2-TTC28、FGFR2-USP10、 FGFR2-VCL and/or FGFR2-ZYMY4.

In some embodiments, of the invention for detecting human gene FGFR1/2/3(especially FGFR2/3) gene The probe combinations of fusion include the probe selected from following one or more fusions for FGFR3 selected from subordinate list one: FGFR3-TACC3、FGFR3-BAIAP2L1、TACC3-FGFR3、FGFR3-JAKMIP1、 FGFR3-TNIP2、FGFR3- WHSC1、FAM193A-FGFR3、FGFR3-ADD1、FGFR3-AES、FGFR3-AMBRA1、FGFR3-ELAVL3、FGFR3- FBXO28, FGFR3-MLLT10 and/or TEL/ETV6-FGFR3.

In some embodiments, the present invention provides a kind of enrichment human gene FGFR1/2/3(especially FGFR2/3) base Because merging target calibration method, which comprises extract nucleic acid from the sample of subject, construct nucleic acid library, and use this The probe combinations of text description capture human gene FGFR1/2/3(especially FGFR2/3) Gene Fusion target, to be enriched with the mankind Gene FGFR1/2/3(especially FGFR2/3) Gene Fusion target.

In some embodiments, method of the invention can be used for various samples, such as tissue biopsy sample, FFPE sample Originally, DNA sample and liquid biopsy blood plasma and cfDNA sample.In some embodiments, subject is not particularly limited, can To include such as normal healthy subjects, the subject with disorders such as cancers is suspected or is diagnosed as.In some embodiments, Cancer of the present invention is not particularly limited, such as can be relevant to FGFR gene mutation (especially Gene Fusion) What cancer (including early, middle and late phase cancer), including such as solid tumor as intrahepatic cholangiocarcinoma and bladder transitional cell carcinoma, such as advanced stage The various samples sources such as advanced stage (III B phase ~ IV phase) intrahepatic cholangiocarcinoma, such as the paraffin embedding fixed through neutral formalin (FFPE) FGFR1/FGFR2/FGFR3 gene becomes in the Circulating tumor DNA (ctDNA) that tissue samples and plasma sample extract It is different.

In some embodiments, the present invention is provided to detect human gene FGFR1/2/3(especially FGFR2/3) base Because of the kit of fusion comprising capture reagent, the capture reagent includes probe combinations described herein.In some embodiment party In case, the kit may include enrichment human gene FGFR1/2/3(described herein especially FGFR2/3) Gene Fusion Various reagents used in target calibration method.

In some embodiments, human gene FGFR1/2/3(especially FGFR2/3) Gene Fusion may include FGFR2-COL14A1, FGFR3-TACC3 and FGFR3-BAIAP2L1.

In some embodiments, the kit further includes one of following or a variety of: 1) building library reagent, such as DNA Polymerase, ligase, buffer and/or quality-control product, 2) other capture reagents in addition to probe, such as sealer capture buffer And/or cleaning solution, 3) solid phase carrier, such as magnetic bead and/or 4) high-flux sequence reagent.

As understood by a person skilled in the art, term " probe " refers to the polynucleotide sequence of variable-length, can be used for The hybridization check of identical, similar or complementary nucleic acid sequence.Sequence as detection probe can mark Labelling groups, such as radioactive group, fluorophor, chemiluminescent groups or electrochemical luminescence group.Polynucleotide sequence is " mutually Mend " refer to the sufficient amount of base-pair to match in the sequence and the target sequence generation specific hybrid for being amplified or detecting Interaction, sequence complementation not necessarily need 100% matched base-pair.Therefore, probe described herein is covered and target sequence Column are not the corresponding sequences of 100% pairing, including for example have at least 50% with target sequence or its complementary series, 55%, 60%, 65%, the sequence of 70%, 75%, 80%, 85%, 90%, 95%, 98%, 99% identity.In some embodiments, spy as described herein Needle covers the sequence for example hybridized under strict conditions with target sequence.Term " under strict conditions hybridize " under the described conditions that This at least 60% (65%, 70%, the preferably 75%) hybridization and wash conditions of identical sequence phase mutual cross.This stringent condition It is known to the skilled in the art, such as can refer to the hybridization in 6X sodium chloride/sodium citrate (SSC) under about 45 °C, Then 0.2X SSC, 0.1%SDS is used to washed once or for several times under 50-65 °C.Term " hybridization " refers to two polynucleotide sequences Between complementary A-T and C-G base-pair formation.

In some embodiments, the present invention provides a kind of for analyzing human gene FGFR1/2/3(especially FGFR2/ 3) automation equipment of Gene Fusion.In some embodiments, the automation equipment may include library acquisition equipment, institute State library acquisition equipment and capture human gene FGFR1/2/3(especially FGFR2/3 by probe combinations described herein) gene Merge target.In some embodiments, the automation equipment can also include high-flux sequence device and/or interpretation of result Device.In some embodiments, the high-flux sequence device is configured to mankind's base of measurement library capturing unit capture Because of FGFR1/2/3(especially FGFR2/3) sequence of Gene Fusion target.In some embodiments, the interpretation of result dress It sets and includes:

Processor；

Memory is stored thereon with instruction, and described instruction executes following one or more steps when being executed by the processor:

1) the human gene FGFR1/2/3(especially FGFR2/3 that measures by the high-flux sequence device is obtained) gene melts Sequencing data is closed,

2) defining sequencing sequence below is inconsistent reading (discordant reads): Insert Fragment size is greater than A pair of of reading (reads) of 1000bp or both-end reading (pair end reads) compares onto different chromosomes, or compares knot Fruit poor direction,

3) defining in all comparison results CIGAR character string containing H and S is editing reading (clipped reads),

4) all inconsistent readings (discordant reads) are extracted and surrounding editing reads (clipped Reads) and source is marked,

5) duplicate removal is read to above,

6) 27-mer for the reading that source in result above is phase homologous chromosomes is extracted,

7) common 27-mer and blacklist 27-mer are filtered, usually the fusion false positive repetitive sequence on chromosome

8) reading obtained in the previous step comprising above-mentioned 27-mer is clustered using algorithm is merged, is labeled as cluster (clusters)

9) local assembling (local assembly) is done using fermi-lite,

10) it is compared again to genome is arrived, is filtered simultaneously, only retain the candidate structure variation of phase homologous chromosomes,

11) the inconsistent reading (discordant reads) and surrounding editing that source is different chromosomes are read (clipped reads) repeats 6) -9) step, retain the candidate structure variation of different chromosomes,

12) two parts result is merged, filtering supports reading (support reads) few as a result, and being annotated.

Detailed description of the invention

Fig. 1 is probe design process example, and wherein Fig. 1 a is FGFR2 introne probe design example: by including The probe that subregion designs 4x tilling is covered to obtain higher capture rate；Fig. 1 b is by fusion partner gene The exon of TACC3 and the accuracy for including subregion design probe raising FGFR3 fusion detection.

The overall flow of Fig. 2 display example experiment.

Fig. 3 display example analysis of biological information process.

Specific embodiment

The purpose of the present invention is to provide the probe sequences and the method for salary distribution of detection FGFR1/2/3 Gene Fusion.This hair The bright FGFR1/2/3 gene that has been directed to devises specific aim primer, and finds that the primer can be with highly sensitive and specificity Extensive FGFR1/2/3 Gene Fusion is detected, especially common FGFR1/2/3 Gene Fusion.In further implementation of the invention In scheme, all FGFR1/2/3 Gene Fusions of the primer combined covering that the present invention designs, thus realize disposably detect it is all can The purpose of energy FGFR1/2/3 Gene Fusion.

Target gene:

FGFR1(NM_001174063): whole exons and 4,5,7,9, No. 17 intrones；

FGFR2(NM_001144917): whole exons and 1,15, No. 17 intrones；

FGFR3(NM_000142): whole exons and No. 17,18 intrones and 3 ' UTR；

FGFR3 chaperone TACC3(NM_006342) the 11st, No. 14 probe covering for including subregion.

Local scope: the appearance of target gene part first is outer aobvious in the upper all fusion forms being likely to occur of COSMIC The introne of sub- front.

Probe length: 120 nt are greater than length in the region of probe length, are completely covered by the way of probe overlapping Target area, the region of probe overlapping are 60 nt.It is less than the region of probe length for length, is covered completely using single probe Lid target area, the center of probe are overlapped with the center in region.For merging hot spot region, by including sub-district The probe that 4x tilling is designed in domain is covered to obtain higher capture rate.Every probe all passes through BLAST and full-length genome It compares；The hit for being 60 or more for length carries out score calculating；Every probe calculates total score according to the quantity of hit, and score is higher Probe specificity it is poorer.Probe of the score greater than 2 is not considered.The single probe combinations in region region may probe combinations In the minimum probe groups of all probe score summations.It does not consider in the region consensus repeat (such as SINE, LINE, LTR etc.) Design probe.For the genome repeat region where FGFR fusion, it is difficult to probe is directly designed, by fusion partner (partner) gene, for example, TACC3 exon and include subregion design probe improve FGFR3 fusion detection it is accurate Degree.Probe combinations appropriate are selected, and are verified, subordinate list one is shown in.Probe design process example is shown in Fig. 1.

The present invention also mentions the kit of detection FGFR1/2/3 Gene Fusion, especially disposably detects FGFR1/2/3 gene The kit of fusion.This kit is mainly used for gene DNA variation detection, and the exemplary compositions of kit are as shown in the table.

Kit exemplary group is at (kit may include following one or more reagents, and can be placed in one or more In a packaging).

The method of the present invention may include that following one or more stages (and the stage can be suitble to make for packet of each stage Corresponding reagent, the reagent can be selected by those skilled in the art, may include various commercialization reagents or reagent Box): nucleic acid extraction, library construction, library capture, the sequencing of upper machine, interpretation of result stage.The overall flow of exemplary experiment is shown in figure 2.It is specific illustrative steps are as follows be described in detail:

(1) the genomic DNA double-strandednucleic acid in blood and tissue samples is extracted；

(2) to handle to obtain DNA for the DNA double chain nucleic acid denaturation of step (1) single-stranded, carries out end reparation, link and PCR and expands Library is built in increasing；

(3) after building library, the DNA library is captured using capture probe described in claim；

(4) it requires to carry out upper machine testing on Fit Models according to sequencing reaction universal kit；

(5) data after sequencing are subjected to automatic processing, remove background noise, the biological information merged according to said gene Analysis method is learned, the Gene Fusion of sample is detected.

This kit can theoretically detect all known and unknown chaperone including FGFR1/2/3 gene Fusion, representative Gene Fusion information such as following table.

Probe of the invention, method, the FGFR1/2/3 representativeness Gene Fusion of product testing.

The present invention also provides a kind of detection FGFR1/2/3 Gene Fusions (including disposably to detect FGFR1/2/3 gene and melt Close) bioinformatic analysis method, specific illustrative method is as shown in Figure 3.

It is verified that this invention advantageously has following one or more effects:

1) the encoded exon region in the region genome target gene FGFR1/2/3 of capture probe capture human genome and outer aobvious Sub- introne handover region, specificity are high；Reduce non-specific capture；

2) sample initial amount is low, and sensibility is high；Gene mutation of the content down to 2% in detectable 50 ng fragmentation DNA samples, or In plasma sample down to 0.7% gene mutation；

3) current all FGFR1/2/3 Gene Fusions are applicable to, do not limit fusion partner gene in upstream or downstream, companion Which companion's gene is, it is possible to find the either totally unknown fused type of the unknown fusion breakpoint of known chaperone；

4) sample type on probation is wide, including but not limited to the patients with solid tumor paraffin embedding (FFPE) fixed through neutral formalin Tissue samples and plasma sample extract plasma DNA (cell free DNA, cfDNA), wherein include circulating tumor DNA(Circulating tumor DNA, ctDNA).

Exemplary description hereafter carried out respectively to five stages of the method for inspection of the invention: nucleic acid extraction, library construction, Library capture, the sequencing of upper machine and interpretation of result.

A. nucleic acid extraction

1.1 illustrate that the extraction for carrying out plasma sample nucleic acid and purifying (recommend nucleic acid to mention according to producer using nucleic acid extraction kit Reagent is taken, see Table 2 for details), cfDNA(cell-free DNA is all referred to as below the plasma DNA after extraction)；

The cfDNA of 1.2 pairs of extractions carries out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument)；(after the completion of this step can in Disconnected experiment is extracted product and is saved in -20 ± 5 DEG C, -70 DEG C or less long-term preservations.)

B. library construction

2. preparing before experiment

2.1 taking-up ends repair buffer and are placed in normal temperature unfreezing, and it is spare that end repair enzyme is placed in ice chest；

2.2 prepare enough Fresh 80% ethyl alcohol it is spare (80% ethyl alcohol total amount of Fresh is prepared by the every sample of 1mL, such as 12 samples build library and prepare 12mL80% alcohol)；

2.3 gDNA fragmentations (only limit FFPE sample): taking 50-500 ng gDNA in 1.5ml centrifuge tube, can less than 50 μ L 50ul is complemented to nuclease-free water, of short duration centrifugation after mixing；50 μ L gDNA solution are transferred to interrupt pipe and be put into and are interrupted in instrument, Fragment length is selected to carry out gDNA fragmentation for the program that interrupts of 200-300bp；(after the completion of this step can interrupt experiments, produce Object freezes in 20 ± 5 DEG C, 70 DEG C or less long-term preservations；

3. end is repaired

3.1 (will can prepare mixed in the PCR reaction tube or eight connecting legs of following components addition 0.2ml nuclease free when sample is more It closes liquid to be dispensed)；

3.2 concussions mix, of short duration centrifugation, and following procedure is run in PCR instrument, and (negative quality-control product and positive quality control product are from this step It is rapid to start to operate with sample synchronization):

4. connection

4.1 take out previous step reaction tube, and gently concussion mixes and reaction tube is added by following components after of short duration centrifugation:

4.2 concussions mix, and following procedure is run in PCR instrument after of short duration centrifugation:

4.3 take out magnetic bead A, and equilibrium at room temperature 30min is spare；

4.4 take out reaction tube, and 43 μ L magnetic bead A are added, is sufficiently transferred in 1.5ml centrifuge tube after piping and druming, is incubated at room temperature 5min；

4.5 are transferred to centrifuge tube on magnetic frame, after standing 2min, clarify careful inhale to solution and abandon supernatant；

4.6 are added 200 μ L, 80% ethyl alcohol, cover after centrifuge tube lid is stored at room temperature 30s, careful inhale abandons supernatant；

4.7 repeat 4.6 operations once, it is ensured that tube wall and tube bottom no liquid residual；

4.8 are maintained at centrifuge tube on magnetic frame, and drying at room temperature 5min(can not make magnetic bead over-drying)；

4.9 21 μ L of addition are stored at room temperature 5min after being resuspended without the concussion of enzyme water；

4.10 are transferred to centrifuge tube on magnetic frame, and after being stored at room temperature 2min, it is anti-to new 0.2ml PCR to draw 20 μ L supernatants Ying Guanzhong；

5. PCR amplification

5.1 choose corresponding sample label and record, and 5 μ L sample labels are added separately into PCR reaction tube, and carry out Label；

5.2 are added 25 μ L PCR reaction solutions I, mix well, run following procedure in PCR instrument after of short duration centrifugation:

5.3 take out PCR reaction tube, and 50 μ L magnetic bead A are added, and room temperature after mixing in 1.5ml centrifuge tube is sufficiently transferred to after piping and druming and is incubated Educate 5min；

5.4 are transferred to centrifuge tube on magnetic frame, and after being stored at room temperature 2min, careful inhale abandons supernatant；

5.5 are added 200 μ L, 80% ethyl alcohol, and after being stored at room temperature 1min, careful inhale abandons supernatant；

5.6 repeat 5.5 operations once, it is ensured that tube wall and tube bottom no liquid residual；

5.7 are maintained at centrifuge tube on magnetic frame, and drying at room temperature 5min(can not make magnetic bead over-drying)；

5.8 31 μ L of addition are stored at room temperature 5min after being resuspended without the concussion of enzyme water；

5.9 are transferred to centrifuge tube on magnetic frame, after being stored at room temperature 2min, draw supernatant into new 1.5ml centrifuge tube；

5.10 pairs of library nucleic acids carry out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument)；It (can be interrupted after the completion of this step Experiment, product are saved in -20 ± 5 DEG C, -70 DEG C or less long-term preservations).

C. library captures

6. capture

6.1 are carried out calculating taken amount and be mixed into the centrifuge tube of new 1.5ml by following principle to DNA library:

1) library of four different sample labels is recommended to blend together a mixing library；

2) library of concentration comparable suggests being arranged in a mixing library, avoids hybridization efficiency difference；

3) total amount in a mixing library is 1.6 μ g, and each library amount mean allocation is (if sample less than 4, guarantees its mixing Library total amount is 1.6 μ g afterwards)；5 μ L sealers I, 2 μ L sealers II are added into each mixing library respectively, it is sufficiently mixed To be centrifuged nozzle with ParafilmTM after even of short duration centrifugation, and 5 apertures are pierced on sealed membrane with clean pipette tips, is put into vacuum Concentrating instrument, vacuum are drained to no liquid and are remained；

6.2 sequentially added into the mixing library after draining 8.5 μ L capture buffer, 2.7 μ L capture reinforcing agent and 3.8 μ L without Mixing fullys shake in enzyme water, and after of short duration centrifugation, concussion centrifugation is primary when 5min during room temperature is protected from light standing 10min() it is heavy molten；

6.3 are fully transferred to liquid in 0.2mLPCR reaction tube, are placed in 95 DEG C of denaturation 10min in PCR instrument；

6.4 take out PCR reaction tube, mix well after rapidly joining 2 μ L capture probes, of short duration centrifugation is incubated for 65 DEG C in PCR instrument Educate (time is no more than 18 hours overnight, and heat lid is set as 75 DEG C) overnight；

6.5 take a certain amount of magnetic bead cleaning solution (2 ×), cleaning solution I (10 ×), cleaning solution II (10 ×), cleaning solution III (10 ×), Cleaning solution IV (10 ×) calculates demand as described below, and it is spare to be diluted to corresponding 1 × working solution respectively with no enzyme water；

6.6 take a certain amount of (by 100 μ L × mixing library number calculating demand) 1 from 1 × working solution after dilution in advance × it washes It washs liquid I and all 1 × cleaning solution II is spare in 65 DEG C of metal baths incubation 2h or more；

6.7 take magnetic bead B to be stored at room temperature 15-30min in advance；

6.8 concussions take out a certain amount of (by 100 μ L × mixing library number calculating demand) magnetic bead B to new immediately after being resuspended It in 1.5mL centrifuge tube, is transferred on magnetic frame and stands 1min, inhale and abandon supernatant；

6.9 are added 1 × magnetic bead cleaning solution of 2 times of volumes, and resuspension fullys shake, is transferred on magnetic frame and stands after of short duration centrifugation 1min inhales and abandons supernatant；

6.10 repeat 6.9 once；

6.11 are added 1 × magnetic bead cleaning solution of 1 times of volume, are dispensed into different 0.2mL according to every 100 μ L of pipe after concussion resuspension In PCR reaction tube；

PCR reaction tube is transferred on the magnetic frame of 0.2ml specification and stands 1min by 6.12, and careful inhale abandons supernatant, and pipette tips is avoided to inhale To magnetic bead；

6.13 take out the PCR reaction tube after overnight hybridization, and it is anti-that whole hybrid products are transferred to the above-mentioned PCR containing magnetic bead B Ying Guanzhong, of short duration centrifugation is placed on 65 DEG C of incubation 45min in PCR instrument after sufficient vortex mixing, is during which vortexed and mixes every 10min Once magnetic bead is avoided to be settled down to bottom；

After 6.14 are incubated for, 1 × cleaning solution I of 65 DEG C of 100 μ L preheatings is added in every pipe, and whole suspensions are transferred to 1.5mL In centrifuge tube (being placed on 65 degree of metal baths for 5 minutes in advance), concussion is mixed, and after of short duration centrifugation, is transferred on magnetic frame and is stood 5s, It inhales and abandons supernatant；

6.15 are added 1 × cleaning solution II of 65 DEG C of 200 μ L preheatings, and concussion mixes, and 65 DEG C of incubations are immediately placed on after of short duration centrifugation 5min is then transferred on magnetic frame and stands 5s, inhales and abandons supernatant.It is primary to repeat this step；

6.16 are added 200 1 × cleaning solutions of μ L I, and of short duration centrifugation after vortex 2min is then allowed to stand on magnetic frame, magnetic bead is complete Remove supernatant after separation；

6.17 are added 200 1 × cleaning solutions of μ L III, and of short duration centrifugation after vortex 1min is then allowed to stand on magnetic frame, magnetic bead is complete Remove supernatant after separation；It is added 200 1 × cleaning solutions of μ L IV, of short duration centrifugation after vortex 30s is then allowed to stand on magnetic frame, magnetic Pearl removes supernatant after being kept completely separate；

6.18 18 μ L of addition are fully transferred to spare in 0.2mL PCR reaction tube after being resuspended without the concussion of enzyme water；

6.19 are separately added into 25 μ L PCR reaction solutions and 5 μ L PCR primers into above-mentioned PCR reaction tube, mix well it is of short duration from Following procedure is run after the heart in PCR instrument:

6.20 take out magnetic bead A from four degree of refrigerators, and equilibrium at room temperature 30min is spare.After PCR take out PCR reaction tube, it is of short duration from After the heart, product is fully transferred in new 1.5mL centrifuge tube；

6.21 are added 75 μ L magnetic bead A, and concussion mixes, is stored at room temperature of short duration centrifugation after 10min, is transferred on magnetic frame and stands 1min inhales and abandons supernatant；

6.22 are added 200 μ L, 80% ethyl alcohol, after standing 30s, inhale and abandon supernatant.It is primary to repeat this step operation；

6.23 uncap dry to dripless residual and magnetic bead it is non-reflective；

6.24, which are added 22 μ L, shakes without enzyme water magnetic bead is resuspended, and of short duration centrifugation, which is stored at room temperature after 5min to be transferred on magnetic frame, to be stood It is spare into new 1.5mL centrifuge tube to draw 20 μ L supernatants by 1min；

6.25 take 1 μ L capture library to carry out accurate quantification (being recommended to use Qubit fluorescent quantitation instrument)；(after the completion of this step can in Disconnected experiment, product freeze in -20 ± 5 DEG C, -70 DEG C or less long-term preservations.)

D. upper machine sequencing

7. machine is sequenced on

7.1 require to carry out on Fit Models according to sequencing reaction universal kit (PCR sequencing PCR) (providing reagent for oneself, see Table 2 for details) Upper machine testing；

E. interpretation of result

8. result obtains and analysis

Data after sequencing are transmitted to mankind's FGFR1/2/3 mutation combinations and test and analyze software, runs software is compared point Sample variation result is obtained after analysis.Specific step is as follows for raw letter analysis process:

8.1 definition sequencing sequences below are inconsistent reading: Insert Fragment size is greater than 1000bp or both-end is read A pair of of read-around ratio to in different chromosomes or comparison result poor direction,

CIGAR character string is editing reading containing H and S in 8.2 all comparison results of definition

8.3 all inconsistent readings of extraction and surrounding editing read and mark source；

8.4 pairs or more read duplicate removal

8.5 extract the 27-mer that source in result above is phase homologous chromosomes

8.6 filterings common 27-mer and blacklist 27-mer

8.7 cluster the reading obtained in the previous step comprising above-mentioned 27-mer using union-find algorithm (cluster), it is labeled as cluster

8.8 do partial assembled using fermi-lite software

8.9 compare again to genome, only retain the candidate structure variation of phase homologous chromosomes

8.10 pairs of sources are that the inconsistent and editing reading of different chromosomes repeats 8.5-8.9 step, retain different chromosomes Candidate structure variation

8.11 merge two parts result, filter, annotation；

8.12 refer to genome and database, and the current reference genome of raw letter analysis process is based on USCS hg19, version This number Version no.:v1.0 (20-Mar-2009 version).Database includes:

1) panel internal reference genome and common SNP 27-mer data file

2) the 27-mer data file introduced by experimentation and sequencing procedure

3) comment file is merged: the GTF file converted using UCSC hgTables 2013-06-14 editions

9. fused data analyzes result judgement:

9.1 analytical parameter setups are as follows:

10. fusion software performance comparative analysis:

10.1 samples selections and experimentation:

Using totally 10,75x2 sample of UDI test, three tools distinguish call SV.Filter condition: autofusion takes existing It has ready conditions.Delly2 uses PASS and PRECISE tag as filter condition, requires breakpoint distance in the SV of DEL, INV type Greater than 1000, the filter criteria after annotation is consistent with autofusion.BreakID does not need filter condition；

10.2 experimentation:

The version of BreakID downloading can not compile, and modify header file and compiling condition while can run after modifying partial code But all samples are all without any result.It is final the result is that using recognizing after IGV interpretation after result after all filterings is merged For the result for being true positives；BreakID not positive reason analysis: the SA tag of this tool dependence bwa mem；It is required that breakpoint There is SA tag at both ends, it is more likely that require to bwa version and existing procedure is incompatible；

10.3 experimental result:

True positives 8, the sensibility and PPV of existing autofusion is all 100%:

11. detection limit (LOD) analysis

Probe sequence is merged using the FGFR1/2/3 of the covering of targeted capture panel described in subordinate list one, is carried out using two cell lines Test, wherein A has FGFR2-COL14A1 and FGFR3-TACC3 fusion, another B has FGFR3-BAIAP2L1 fusion.Mixing ladder Degree is following to be indicated, each 15-20 sample of gradient differs.Gradient concentration is quantified by digital pcr:

Using 95% recall rate as standard, current fusion LOD is about 0.7%, and detailed results are as shown in the table:

The specificity of probe combinations and method detection FGFR Gene Fusion of the present invention and sensitivity are significantly higher than existing method at present And probe combinations.

The detection limit performance of the present invention and prior art detection Gene Fusion is referring to following table:

Name of product	Manufacturer	The fusion declared	Detection limit performance indicator
				10 gene mutation combined detection kit of the mankind (reversible end termination PCR sequencing PCR)	Xiamen Ai De	ALK, ROS1, RET	The detection of 30ng DNA sample is fused to variation abundance 1%
Human epidermal growth factor receptor/ALK/BRAF/KRAS gene mutation combined detection kit (reversible end End terminates PCR sequencing PCR)	Fire stone in Guangzhou	ALK	The detection ALK fusion detection of 50ng DNA sample is limited to variation abundance 10%
				EGFR/ALK/ROS1/BRAF/KRAS/HER2 gene mutation detection kit (can Inverse end terminates PCR sequencing PCR)	Nanjing generation and	ALK, ROS1	250ng DNA sample detection ROS1 is fused to variation abundance 1%, ALK is fused to variation abundance 2.5%
Human epidermal growth factor receptor, KRAS, BRAF, PIK3CA, ALK, ROS1 gene mutation detection kit (semiconductor PCR sequencing PCR)	Tianjin promise standing grain	ALK, ROS1	30ng DNA detection fusion detection is limited to 100 copies
				Mankind's FGFR1/2/3 gene mutation combined detection kit (high-flux sequence method) (present invention)	Thinking enlightening	FGFR1,FGFR2, FGFR3	30-60ng cfDNA or 50-200 ng gDNA detects FGFR Fusion detection is limited to variation abundance 0.7%

In summary, the present invention provides a set of high-flux sequences to detect human gene FGFR1, FGFR2, and FGFR3 gene melts Capture probe, detection method and kit and application are closed, has biggish promotion in performance, testing result shows of the invention Detection architecture disposably can accurately detect the fusion variation of FGFR1/2/3 gene, and detection limit can be down to 0.7%.It therefore also can be wide It is general to be suitable for various samples, including such as tumor tissue biopsy sample, FFPE sample, DNA sample and liquid biopsy blood plasma, The detection of cfDNA sample.

The FGFR1/2/3 of one targeted capture panel of subordinate list covering merges probe sequence

Note: genomic data version number is UCSC hg19, and the Genbank number of corresponding genome version is GCA_ 000001405.1。

Exemplary embodiments of the invention are illustrated through the foregoing embodiment.The embodiment is to each technical side of the present invention Case does not constitute a limitation.It should be understood by those skilled in the art that: it can modify to the technical solution of foregoing embodiments, or Equivalent substitution of some or all of the technical features.These modify or replace the model still in technical solution of the present invention In enclosing.

Sequence table

<110>Co., Ltd, Shanghai thinking enlightening medical test institute

<120>FGFR Gene Fusion probe, detection method and kit

<130> IB191545

<160> 624

<170> PatentIn version 3.3

<210> 1

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 1

cttgtcagag ggcaccacag agtccattat gatgctccag gtggcataac ggacctgagg 60

ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 120

<210> 2

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 2

ggaaatgcca aagggataca ttgagggtcc agaggaaaat gcaggcccca tgacaatgtc 60

ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 120

<210> 3

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 3

ggcaccccgt ggcacctgcc ctccatatca gagcctggtg gcacagggcc ccaggctgca 60

gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 120

<210> 4

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 4

gggttggcta ggacaaggcg tggattgccc ccctaccagc ccgttcacac tctgcaagct 60

ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 120

<210> 5

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 5

ggcagatggg gtgtaaagac tccagaagtc tccactgtga cgttcaagat cattcgtgat 60

ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 120

<210> 6

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 6

ccggacagat gtgccttctg caaacactcc caaatacacc aagaatgttc ccaactgtgc 60

acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 120

<210> 7

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 7

acctctccta ttacactaag aatccagccc ccactgtcct tgacagctca tttcaaaaac 60

catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 120

<210> 8

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 8

catctcctcc agtccagtct ctggtcagaa ctgtgctcac cctttcctct ctgcctacca 60

aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 120

<210> 9

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 9

aagcactgat cacagtctac tttttacggt agctgctcat ggacatttgc ctcctatcag 60

actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 120

<210> 10

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 10

actgcaaaat ttttatgggc aggaacgcta tcttgcagca tttaaagcat ggtgtcttgc 60

atgtagtgag tgcatggtga attaatttaa aatatttctg gctgggtgcg gtggctcaag 120

<210> 11

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 11

gtgagactcc gtctaaaaaa aaaaaaaaaa tgcttacacc ctttaagtta gtaatttcac 60

ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 120

<210> 12

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 12

ttgtaacaat ctgtcctaag gaagcacgct taaaaaaatg ctgacagaga ttcactgcaa 60

cattatttat tatggtgaaa aaccagaaaa tacctaatat cacaaaatag ttcattaaat 120

<210> 13

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 13

gcccagccaa attatgggat tttaaaataa tggaatctta tacagtcatg aaaaattatg 60

tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 120

<210> 14

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 14

tttcaaatat tcatgcttga aatatatcag gaaaagactc atgttaacaa atgacaaggc 60

aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 120

<210> 15

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 15

aaggaaatta catacatgat gtgatcaact atgtaaaaac catattacag aaagaagact 60

gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 120

<210> 16

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 16

gaagaaatgt ttaaacttta tttgcatttt tttgtattta aaaattttaa tgaataacct 60

gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 120

<210> 17

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 17

gtataggtgg aaagtattac ttaaaaaaat gaaaagcatg taatcaggac ttcctaactc 60

ggcctcccct gttcccatta ctctaacttt cgcatgcaca cacacgtacc ttgtagcctc 120

<210> 18

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 18

acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 60

ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 120

<210> 19

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 19

ctgtgcctgg cctatggccc cttctcttaa tcaccactca ctcacaactt cagtctgaag 60

taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 120

<210> 20

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 20

acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 60

agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 120

<210> 21

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 21

ccactgcgcc cggccttctt tcccttttac aagtacagaa caagtggact tcaaaagtcc 60

aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 120

<210> 22

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 22

ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 60

accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 120

<210> 23

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 23

cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 60

gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 120

<210> 24

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 24

cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 60

gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 120

<210> 25

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 25

acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 60

accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 120

<210> 26

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 26

ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 60

atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 120

<210> 27

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 27

tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 60

acagagtggt ccaaagacca tggtaggcca ggaggcccaa cttctaggac tgacctattt 120

<210> 28

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 28

acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 60

ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 120

<210> 29

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 29

accttccctg gattaatgga gcattcaggg acaatgaatg tctgggagtg agaggaaaaa 60

ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 120

<210> 30

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 30

ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 60

cgtaactcag gacacagggc taagactggg aaacgcttgg tggaaaggct ctggtttcgg 120

<210> 31

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 31

taccatttgt aacacagcaa aaccgtgtcc ccgtgagtcc tcacatggat ccacacaaag 60

ccaaggaaca cacagaaggc agagagggaa cccacagaca ccaacacaat accaaaccaa 120

<210> 32

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 32

tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 60

ccaatgacct acagagcagt tttagcacca gcagcaaaga gaagagaagg gagggccagt 120

<210> 33

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 33

gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 60

gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 120

<210> 34

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 34

gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 60

tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 120

<210> 35

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 35

atctctttgt cggtggtatt aactccagca gtctagaaga gacaacggaa gcaaaatgga 60

caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 120

<210> 36

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 36

tccccagcac agggtcccat ccatctcccc attacctttt gccacaggtc tggtgacagt 60

gagccacgca gactggttag cttcaccaat ataattggaa accttacaca catactcccc 120

<210> 37

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 37

tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 60

aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 120

<210> 38

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 38

gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 60

accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 120

<210> 39

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 39

gctctgggcc tctgtcacat tgaacagggt cagcacctcc gcatccgagc tattaatccc 60

cgaatgctgg aacaaaccaa cgacacacca gtcagttggg caaagctgag atgtggccac 120

<210> 40

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 40

tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 60

agactggtgc catgattacc ttcaagatct ggacataagg caggttgtct gggccaatct 120

<210> 41

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 41

accagcctcc caggccgggt atttttccat ggctctgggc actagaaata cagtagggtg 60

gcaaggacag tccagtactg gcttccccgc ctgccccagc agcttggggc ctacatcaaa 120

<210> 42

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 42

acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 60

agggaatgcc taaccccttc tctctctttt ttaagagatg gggtaccatt ctgtcaaccc 120

<210> 43

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 43

tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 60

acagtgtctc acgcatacgg tttggtttgg tgttatctgt ttctttctcc tctgaagagg 120

<210> 44

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 44

ctgcccgggg aaagccaaga gagacaggca gggtggagag gagcagctgg tcaggctcag 60

gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 120

<210> 45

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 45

ggaagaaaat gccattactc ctcgtcctca tatggcttag aatttatata tggcatttgt 60

ttttccaact cttcgtaaga gatgctctta tcatgcaccc catttgccag aaagatcgta 120

<210> 46

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 46

atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 60

ccatctactt tctgttacct gtctgcgcag agggatgctc ttggccagct tgtgcacagc 120

<210> 47

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 47

ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 60

tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 120

<210> 48

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 48

agctcacctc cactttgtga cctctgttac tagtcctggg ggatgtggct agatccctac 60

tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 120

<210> 49

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 49

atatctctga gacagctaag ctaggggagg atgaggtcta actctgggac aaagaggtaa 60

acaactttcc cagcttctgg ttccttcctg ttgcgagatc cactggagat ttaggcaatt 120

<210> 50

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 50

gagcagggcc caggccagga cctggaggtg tcttgcccat ctgcccaaca cctgtgagca 60

gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 120

<210> 51

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 51

tttgcaaata ctttgcctta gcttatggat tcctaactcc caaagcacct tccagaatga 60

gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 120

<210> 52

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 52

tgagatggag tgtgtgtgcc tgaagcgtga ggaatgatcc cattcggggg caactgagcc 60

tgcccacagg aacttgagcc cccgagacag tggtctcctt cccagtagac tggcccacga 120

<210> 53

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 53

gcacaggctt ctgagggaac ggtcattctt gcacacacct gtgccatctg taccatttcc 60

tcccgtgggg aagggtgggc acacagggga caagggtatg gtatggctga gatggtttgg 120

<210> 54

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 54

caagcacagg acatgagacc tctaagagac gcagagcaag ggaaggagac agaaagaagg 60

gggactagag gaagaaatgc tccctgacat cttctttgca acaaggaaca aacgaaaacc 120

<210> 55

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 55

accaggcagc aaaacctgtt agaggaagag ggtgagaccc tgagggaaga actccggcag 60

acctgagaag acgatggctg gttacgaccc ttcaccagat gctggcagcc actggcccat 120

<210> 56

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 56

tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 60

attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 120

<210> 57

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 57

cagcagtgtg aaaatggact aatacaaaca gttctcttct ctaccctgca tctctgcact 60

tctaagcagc ccttggggac agttcaaatt catgctattg gctgcacatt ctgtccaaat 120

<210> 58

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 58

tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 60

acatgcacac gcatacacac acgcacatgt gcatacacag ccagcagatg cgagtatgca 120

<210> 59

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 59

ggccgaacca gaagaacccc agagttcatg gatgcactgg agtcagcaga cacctgcaag 60

gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 120

<210> 60

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 60

ctgtgatggg cgagaggaag cagcgatggg ccgggcccct cctccctgct cagggaggtg 60

cgtgcacgca gtggggacgg cctgagctct ggctctggca cgggcagcct tacctggttg 120

<210> 61

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 61

gcaatgaaaa ggcaggggtc caaatgcctt ccttgtgtag ctgaccccaa agccccagtg 60

acgttgctct caaagcttat tacagaccag caccacccat ccctgcagcc ctctgttccc 120

<210> 62

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 62

ctgccacaag ctggcctttc tggacttcac tcacctttaa aataagcaga ccaaatgcac 60

ctcttccagc ttgacatttt ataactgatc attgcagtaa caactctgtt ctcctgactc 120

<210> 63

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 63

ccgagcatgc gggcggtgag cggcatggag aaatggggcc ggcgggcagg ggctgtttgg 60

tttcaggtaa agatagccag aaggtgaaca caggagggat ggcgggggct gcagacacac 120

<210> 64

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 64

aggacttcct cccaatccat gtcccaggag tccggaggac ttgcagtgtg atatggaggg 60

tgcaggaaat tcacgggcta cactagcttg aaacagactc tagagcactt agttcatgat 120

<210> 65

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 65

gcacgttgct cccgtgtaac tttcaggcag gcccagggag cgaagggaga tgttaagtga 60

gatttattac ctttcccttt tagttttttt tttttttttt ttttttaacg cagagtctca 120

<210> 66

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 66

aaagcatttg attaaaaaaa aaaaaaaaaa aaaagaatca ctgcttcaaa tggaatgttc 60

tatttgtgtt taacccactc cctccaggac tggagcaggg gttgtgagtg gagacagtgg 120

<210> 67

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 67

gaagagtggg gtcaccctag agcaaggagg ggggacgggg tgactccttc ccattcttag 60

tcagggcttc ccaacaatgg cagaggcaca tgtctgtgta tctgatgttc tttcctcaac 120

<210> 68

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 68

gtgcctggca cttagcagaa caggcaccgt gaccccatgt gccctcctct tcctcccctt 60

tcagccttcc cctcccctct taaacccaat gcccagaccc aaagggcagt aagataggaa 120

<210> 69

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 69

attccactgt gccttgccca gaagccagaa aataaggccc aaagctataa attagggaca 60

atggagaggg cagggcatta gaggcccaga gagagaggtg gtgctgagtg tgcaaatccc 120

<210> 70

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 70

aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 60

ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 120

<210> 71

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 71

tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 60

aaggtttatc ccgccgatag agggacggcc aagaatgtac gtccagcctg ccccggagct 120

<210> 72

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 72

gtgagtgctg gctctggcct ggtgccaccc gcctatgccc ctccccctgc cgtccccggc 60

catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 120

<210> 73

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 73

ggaggatccc ctccaagcct aaaaggttgt taatagttgg aggtgattcc agtgaagata 60

ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 120

<210> 74

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 74

tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 60

ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 120

<210> 75

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 75

tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 60

tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 120

<210> 76

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 76

cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 60

ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 120

<210> 77

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 77

ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 60

cccatgcaag cagaggacca gggccttttc tggcaccgca gttttgtttt aaaactggac 120

<210> 78

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 78

tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 60

caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 120

<210> 79

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 79

agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 60

ggagtacctg gacctgtcgg cgcctttcga gcagtactcc ccgggtggcc aggacacccc 120

<210> 80

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 80

acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 60

gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 120

<210> 81

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 81

ttttatttcc tttgtccttt ttcaggagaa ttagatttct ataggatttt tctttaggag 60

atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 120

<210> 82

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 82

gacgtgaagg gccactggtc cccaacaatg tgaggggtcc ctagcagccc accctgctgc 60

tggtgcacag ccactccccg gcatgagact cagtgcagat ggagagacag ctacacagag 120

<210> 83

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 83

ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 60

tgtatttgtt gtagacttaa cacttcttac gcaatgcttc tagagtttta tagcctggac 120

<210> 84

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 84

gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 60

ttattagatg ttacaagttt atatatatct atatatataa tttattgagt ttttacaaga 120

<210> 85

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 85

tgctaccttt caaagcttgg agggaagccg tgaattcagt tggttcgttc tgtactgtta 60

ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 120

<210> 86

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 86

cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 60

ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 120

<210> 87

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 87

tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 60

tttgttaacc cagcgacgaa ctttccgaaa aataaagaca cctggttgct aacctggccc 120

<210> 88

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 88

ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 60

acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 120

<210> 89

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 89

atttattttt tggacttcaa agcaagctgg tattttcata caaattcttc taattgctgt 60

gtgtcccagg cagggagacg gtttccaggg aggggccggc cctgtgtgca ggttccgatg 120

<210> 90

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 90

acatatatgg aagaggaaaa ggctggtaca acggaggcct gcgaccctgg gggcacagga 60

ggcaggcatg gccctgggcg gggcgtgggg gggcgtggag ggaggcccca gggggtctca 120

<210> 91

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 91

caacggtctc ctgactggtg ctgcagcacc gaggggcctt tgttctgggg ggacccagtg 60

cagaatgtaa gtgggcccac ccggtgggac ccccgtgggg cagggagctg ggcccgacat 120

<210> 92

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 92

ctttggtctg tgtgtgtgtg tgtgcgtgtg tgtgtgtgtg tgtgcacatc cgcgtgtgcc 60

tgtgtgcgtg cgcatcttgc ctccaggtgc agaggtaccc tgggtgtccc cgctgctgtg 120

<210> 93

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 93

ctgtatattt gtaaagctat ttatgggccc ctggcactct tgttcccaca ccccaacact 60

tccagcattt agctggccac atggcggaga gttttaattt ttaacttatt gacaaccgag 120

<210> 94

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 94

gtggcaccgc ctccccacct ccaggctttc ccacttccca ccctgcccct cagagactga 60

aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 120

<210> 95

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 95

aattacgggt acctgaagat gggagccttt accttttatg caaaaggttt attccggaaa 60

ctagtgtaca tttctataaa tagatgctgt gtatatggta tatatacata tatatatata 120

<210> 96

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 96

catcctgccc cccagagtgc tgaggtgtgg ggcgggcctt ctggggcaca gcctgggcac 60

agaggtggct gtgcgaagag gggctcggtg gcacagcgct caccccgcct cccgccagca 120

<210> 97

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 97

ggctccggcc tctgcctttg caccacggga catcacaggg tgggcctcgg cccctcccac 60

acccaaagct gagcctgcag ggaagcccca catgtccagc accttgtgcc tggggtgtta 120

<210> 98

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 98

ctgggccctg agtctgggca gctgtccctt gcttgcctgc agggccatgg ctcagggtgg 60

tctcttcttg gggcccagtg catggtggcc agaggtgtca cccaaaccgg caggtgcgat 120

<210> 99

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 99

aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 60

tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 120

<210> 100

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 100

caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 60

agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 120

<210> 101

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 101

gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 60

cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 120

<210> 102

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 102

ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 60

cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 120

<210> 103

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 103

aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 60

gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 120

<210> 104

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 104

gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 60

aaggagggtc tggagaacgg tctgcaaggg agcggggtgc gaggagagcg acggaggctg 120

<210> 105

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 105

ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 60

ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 120

<210> 106

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 106

tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 60

tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 120

<210> 107

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 107

tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 60

tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 120

<210> 108

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 108

cttagtccaa ctgggcttta gaaagctaaa ctgttggagg agtctgctca cagctccgta 60

tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 120

<210> 109

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 109

agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 60

ctacgcatgt ctcacaagac aaccaaggac aaggggcttc tagaaggaag ttcttacctc 120

<210> 110

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 110

tttctgctgg gctggaggtg agcagtctcc caccctcact gtcgctcttg ggaagaaagg 60

agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 120

<210> 111

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 111

aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 60

tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 120

<210> 112

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 112

caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 60

tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 120

<210> 113

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 113

cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 60

gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 120

<210> 114

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 114

gggggcaaaa agtgcagacc acagcacaaa cagcctcagt gttatcagaa aagagcccat 60

ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 120

<210> 115

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 115

agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 60

caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 120

<210> 116

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 116

caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 60

attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 120

<210> 117

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 117

gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 60

ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 120

<210> 118

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 118

gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 60

ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 120

<210> 119

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 119

ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 60

cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 120

<210> 120

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 120

acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 60

cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 120

<210> 121

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 121

tatgctttcc ataattataa aaggcactct atctgcatat tgaaggagcc actgacatgg 60

ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 120

<210> 122

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 122

attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 60

caaactttag agaatgatta aaacaatcac aagaaggggc tcatcaatct caggcttcag 120

<210> 123

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 123

gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 60

ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 120

<210> 124

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 124

ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 60

ccttccgccc gtctgctctg gggtcggaga aacgcgccca gtcagcggga atgcgcccgc 120

<210> 125

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 125

tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 60

tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 120

<210> 126

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 126

ctccgctgaa tctcaagccg ttccatcatc ccgctagctg gcactagaaa tttgcatttc 60

agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 120

<210> 127

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 127

ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 60

gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 120

<210> 128

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 128

caccccaggg tgacacttcc tccgacttgg gtgcccacag acgactttgt atggcctgag 60

ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 120

<210> 129

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 129

atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 60

aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 120

<210> 130

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 130

gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 60

ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 120

<210> 131

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 131

cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 60

tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 120

<210> 132

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 132

tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 60

taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 120

<210> 133

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 133

aagctttcta aattgggaac ttgtgggtct ctctgcctcc actgggctgg cacagtgggg 60

cagttctgct cacacatcca gaagccaaca actattctat gacatgtggt tttgctgaga 120

<210> 134

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 134

attctgtgaa ccacaggaat gaaccacatt ttctaagact gggggtggca gggaggcctt 60

taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 120

<210> 135

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 135

cactaaagga aagagattgg cgagtcaggg aatcttcccc aatgccaaat cactccagcc 60

caagtgggat aaaaccattt ttcagcctct caaatgtgcg caaacagagt tctttttcat 120

<210> 136

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 136

accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 60

gccctgagtt tgaaagcagg tttgccttta agggtaggtt ttattgaatt gggagaaagg 120

<210> 137

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 137

cctgcctcgg cctcccaaag tgctgggatt acaggcatga gccactgcgc ctgaccaact 60

tttcccagtt tctcaatgaa gccataaact ttcagatctg ataggaaaaa aacagggata 120

<210> 138

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 138

ggtgaatact gttcgagagg ttggctgagg tccaagtatt cctgaaagaa gggaagagag 60

acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 120

<210> 139

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 139

gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 60

aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 120

<210> 140

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 140

gtggtccccg agcgggcctc ctgcgccagg tccgtggtcc gcgagggtcg gagccagcgc 60

cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 120

<210> 141

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 141

cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 60

tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 120

<210> 142

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 142

aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 60

tccatatgca aacctactgg ctaaattttc agagtataag ttagaagagg acactcaact 120

<210> 143

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 143

acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 60

ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 120

<210> 144

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 144

agccatttcc aggtccaaac cccacggcag ccccctcccc aaaactctgg aaggcggaga 60

cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 120

<210> 145

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 145

ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 60

ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 120

<210> 146

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 146

ctatccacta tcgcctccac cgccctcaat gagcagaagc aacgtgacct tagtttcaag 60

ggcaactaca aaagcaagtt aaaaatgctc gggttggcat aaccaacctc gttcccttct 120

<210> 147

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 147

tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 60

acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 120

<210> 148

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 148

gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 60

tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 120

<210> 149

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 149

tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 60

gatccataaa attttactag aagacagact agaacacatc tatttacata ttgtctgtgg 120

<210> 150

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 150

cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 60

atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 120

<210> 151

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 151

ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 60

aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 120

<210> 152

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 152

agaccagcaa aatcaaaaga atcccaggaa ggctacagaa aattcccacc agtttacact 60

tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 120

<210> 153

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 153

gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 60

agaaagtgtc ctttgctccc caaggcatcc agtgctggtg ctgcaaaaag ccctctggat 120

<210> 154

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 154

taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 60

accagggaga caatggagtt ttaaatgttg atagcgtttc acaccaaaca cataatgggt 120

<210> 155

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 155

gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 60

cgccgacggc ggctgcgggc gaggggcgcc caggcaccgg agccgtcgcc tccccagcag 120

<210> 156

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 156

agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 60

acaggatcac ataggatagt actgtggtaa cagaagctgg tgttagaaaa cttcattgta 120

<210> 157

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 157

aaccggtaac atgttgtggg tccccagacc ccctttaagt agttgagact atgaatattt 60

attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 120

<210> 158

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 158

tttatatcca ccagacaata tggcattctg cttttttatt ttagggtact agatacaaat 60

ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 120

<210> 159

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 159

taatacaaaa atctctaaac tctacatttt ttatgtaccc actcgatgtt tcaagttatg 60

tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 120

<210> 160

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 160

ctactttgag gctacagtgg cgaagttgca acagagacca cagggccggc aaagctgaaa 60

atatttactc cctggccctt tacagaaagc agttgccgac ccctgttctg aagctatgaa 120

<210> 161

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 161

tcattgattt actaagcatt tgcagaatgc aggggaattt tctacctcta acacgacttc 60

aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 120

<210> 162

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 162

ctatgcagcc acagagggag gacacggagc gtcctccaca atgctagtcc ttccgcgccc 60

cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 120

<210> 163

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 163

tctgagaaaa atcatgattt gagaggaacc catagttgaa ctatctgacc ctatggaaaa 60

agaataaaca agaccacaga ctccaaccaa cagccaacag gggagtgtgt gtgtaaaaca 120

<210> 164

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 164

tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 60

gagtctacac cacctcctca ctagagccct tgcttcaaat gcgagcccca gcaaaatgaa 120

<210> 165

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 165

cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 60

aatggaaccg tttttcttga gcatatggta atgattttca gaaggaaaga aacttcgatt 120

<210> 166

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 166

cagggtttga cagggagggg ggcgtcaacg ccagaagcag atgtcagcat ctggatgggg 60

gtagcagagg cagaacttcc agaacgccgg ccgtcagcac gcacccacct cccccatcac 120

<210> 167

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 167

atgtaaacag gttcaaaaaa ggcttagaca tactcatgta tgttaaatcc ataatgcact 60

gttaaaggaa acttcaggaa aatatcggtt aagtttcaaa gttttgaagg ttcgagattc 120

<210> 168

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 168

acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 60

gtagctacag gactcagata cgtgcagcca cttcagtgag gcaatgtgtg ctctcttcta 120

<210> 169

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 169

gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 60

tcagaagata atttcggatc tatttctaca tacatgtggg aggaaagtac acaaacattc 120

<210> 170

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 170

tagcttttct cttggaatcc atcataaatc aactattgac aggtagagaa caataaaacc 60

tagttgagct ataggtgctg ggcaggcaac tcggcttcac acggacctaa ataacccgct 120

<210> 171

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 171

agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 60

cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 120

<210> 172

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 172

gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 60

gcagcctcgg ggatgtccct ctgtctttgg tttcatgagt cagaactcac aaaatgaagt 120

<210> 173

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 173

tgtcaggatt cattttcaaa tggagaagtg tctttcagtc ttccgccaag cagccactta 60

cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 120

<210> 174

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 174

gggcttggct gcggagcggt ccccatgcca ggcctgtgct gggctcttca atttaccatg 60

acattcccaa aacccccgcc ctccaagaag gcagaccaat acagtgccct ccgctttggg 120

<210> 175

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 175

ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 60

agaaaacagt gtatgtttat atacacctag aaagaggctg caaggcatat agcattacca 120

<210> 176

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 176

cccgcccgga ggacggtgca cccggcgtcc cgggctcccg tggcgcccac gtccccggct 60

ggcgaagctc cccgaggcgt cttgcgcggc gattaccttg aatggcaacg ctcctccgcg 120

<210> 177

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 177

caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 60

gaactagaaa aagtcggtcc agttcgcccc ctagccccag gcgcactgcg ctccctccag 120

<210> 178

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 178

tatctgctaa atccttccat cctcacctca gagtcccagg tgataccacc agaccggtcc 60

acagaaatgt gtgaggctaa tctttttaaa agcatacttt taagaaacag agataaaaca 120

<210> 179

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 179

ctgacccagg accacaaagc cctcccggtt taatgcttgc gtccaatcag aacttgcgag 60

cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 120

<210> 180

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 180

tgtgtgtgta tgcacggtgt gtgtgtgtac atggtgtgtg cgtgcacggt gtgtgcatgt 60

gctttcagat agcttgaaag atttttcctc ttaacttttc tttcctctta gtcaagagcc 120

<210> 181

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 181

acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 60

ggtccatttt cacctcggca caagcctgcc cccaaagaca ggcagagtga caagcaggaa 120

<210> 182

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 182

aaatcactct aaaactattt cctaaatcag aaattaagtg tattattttc cttctaaaat 60

acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 120

<210> 183

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 183

ggaatgttgg caactagaca tttttaaaga cacacacaca cacacacaca cacacacaca 60

caaaaccctc taattcatca ccagggttgg caagagaaag agtccccatc ccagcatccc 120

<210> 184

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 184

cattttaatt gacattccag ggaaagagag aggggcgcct gattgctttt ctattcaaac 60

taaacaggct tttcagatga aaatgagtcg agcccggtgg aagatttaca gggatttggg 120

<210> 185

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 185

tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 60

ggggcacctc ggagatgacc ccgggaaccg gcctgcgggg ctgtgccacg ggcaggcgaa 120

<210> 186

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 186

acgttttatt tcatcttggg tcaggataac aaggtgaata cggttcgaga ggctgactga 60

ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 120

<210> 187

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 187

agaaggggaa tgtatcagtt ccaacaacat cagcattaac cacacaactg gaaacaaaca 60

cgcccaccac atcaaaagag aactttttgc ttacaatgat aaaaacgaaa ttttgtccta 120

<210> 188

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 188

ctaaatttgc acagtgagtg acagacgagt gtttattttg ttggcaagga tcataaatta 60

ttattatttt ctttttgaga tgaagtcttg ctctgtctcc cagactggag tgcagtggcg 120

<210> 189

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 189

cacgcagggc cctcggcttt tttccccagc gcggccccgt ggccgaaaaa aatgagcgcg 60

caagttagaa ctcgcctgcg ctttcgacat ctcccagccg ctgcggcaga gcaggcaggc 120

<210> 190

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 190

aggaacaatc ctaagtcctc gcagaggttt caccccaggc tccttccccg gcctgctcgc 60

ccacctgtcc ctgcccagag gagcctcctt ggctccaggc agagaaaggt ggacagggcg 120

<210> 191

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 191

agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 60

aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 120

<210> 192

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 192

ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 60

tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 120

<210> 193

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 193

catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 60

tttttttttt ttttgagacg gagtctcgct ctgtcgccaa ggctggaatg caatggcatg 120

<210> 194

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 194

cagcttgggg gtgctcccca ccaccacccc ctttcagggc agcacttctt tcatttgttt 60

gcctgaaata tcctgttttt ctcccccttc tcggggcgct aaagagctca gagaaatctg 120

<210> 195

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 195

ggctaaagat ggtctttaac attggtaagc ggttgaaaaa aaaaatcaaa agaagaagaa 60

tatcttatag gattaaaatg aataaaattt atatacaatt atataaaatt ccaatttcag 120

<210> 196

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 196

aggaataaag aaaacctctg cttcaaagat agcacaggtg aaacggtgtg attcacagca 60

aaagcaagaa aagtatgtgt gcagtgtgtg tgtgtgtaca cggtgtgtgt gcgtgcatgg 120

<210> 197

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 197

ggtccaagta ttcctgaaag aagggaagag aagagtttta tttcaaacac aaactcttga 60

gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 120

<210> 198

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 198

cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 60

acgcgggagg agttgtcacc ctccctctga tggatggctc ttctcggccg cccgcccccc 120

<210> 199

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 199

attttctgaa ttacatttta ctattgtagt ttcggggata attgaagggg aatacttctt 60

cattcagtgg gtaatttaac agatgtaact gattactctc agaagctctc cgagttgaaa 120

<210> 200

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 200

cttccaccta aggggtgatc tgtccactgc tggctcggcc actgggatca cctggtgttt 60

tgttcctggg tagatttcac atcaggaaag tcaacagtgt ccggctcctc caaagtgcag 120

<210> 201

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 201

tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 60

tttctctatc ttaagatttg actagacttg atgggtttat catctttcct gctcagagca 120

<210> 202

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 202

cagctgtatt catttaagtg caatctcttc ctttatccag cacaggccac agtcagacgc 60

ataagataaa gctgctgagc taagacttca tatcactaca tttgattatt actttttaaa 120

<210> 203

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 203

cctaaggcga caggtctgca cagttcctca caccagtgga caaaagtatg gtgcgacctc 60

ctaggcagga taattaggtc cccaggctcg gtgttgggaa tacaggccca gcatgccgct 120

<210> 204

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 204

acccatctct tcgcttaagg cctccctact tctccccagc actgagtagg agcgggagct 60

gtcaaggggt caaggctagg gggaaattcc tcaacaaatt gaaatctcaa aaaacacaca 120

<210> 205

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 205

ggcggccaca atcccggctt cctgccttca ataccataat ccttcctgga gtctgagccc 60

cagtgaaagc cccagcgtgt ttatttttcc ccttgaaagg gaactctctt ctcagcctgc 120

<210> 206

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 206

cgaagagtga acacaactgc agcgcattca acgcagctga tccaaaaagg acttcgaagc 60

ctgaattccc ttcccctgtg actaaatggc agtccagggc tgctaaatgt cttcaacctc 120

<210> 207

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 207

tcagtagatt ccaagtctac agttaaagga acttatacta gaaacaacaa ttttggcaga 60

agaagaaagt tggtttcttc ccccccttga accgttcctt tcctttcaac ttctgaaggt 120

<210> 208

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 208

acttggggga ctttaacttt tttcttgttg agacaattgc atggtttgga gttagtttcc 60

tgtgtaaaag aacactattg tgcatcctca ttaaatatct caaaatacat tcattaacaa 120

<210> 209

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 209

atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 60

gcttcccctt tcccactctc ccacataccc cggactgctc caccagcagc tacactgaag 120

<210> 210

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 210

tgtagaaggg tgaggaaact gagcagatgc aaattctgat gaataatgca ggaatttatt 60

aataatgatg atgtaaatta tgtaaattat agtcatggta tgtgattgga ccaataactt 120

<210> 211

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 211

tcataggatt tcaacactgc tggagcctcc ccaaatctca tcataaaaac tgctcctttt 60

agctttgact gaggacaaaa atggaggagg gaaaagaata tgccacatca tttccaaagg 120

<210> 212

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 212

ttcagtatca acggacccac tctacatgta aatatataaa taaatcactt tgagcataga 60

catgagatca ttaaaataga actttagtgc ttttcccggt ttctttcttt tttattttta 120

<210> 213

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 213

tgccacccct ggccatcgcc acccctacgg gatgtcaccg gccccacccc agcggccccc 60

tcccgaaaaa ggggtctccg cagggcctgg atggtacgga agttcccagg acacacggat 120

<210> 214

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 214

taatattctg ttcacccatt tgatccaagg gaaagagaat tttccaagaa agcttaatat 60

attccaagca actttaaatt cactcagtac tctctgtgac taagggtaca gtaaaatgca 120

<210> 215

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 215

ccctcctcgg cttcccaaaa tgctgggatt acaggcgaga gcataaatat tttagggaaa 60

ataaatattt taacaaaagg tacatgtttt tatgtaaaaa tcatgtaagt tgctcattgt 120

<210> 216

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 216

gatgtgggta ttggacgtgg gacaggacag gagaccccta gaaggtgaac agagacccag 60

cccacctgac ttccacgaag acctccctga gaccacgtct gatgtacccc aggctgtcct 120

<210> 217

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 217

ttccatcaag caaatgatga cgaacattat cagtttaccc actaatgacc aaagcacaga 60

atcaaacttg cacaggaatc caagcttttt cccatgcaca ccaaaatgca agtgaatact 120

<210> 218

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 218

ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 60

tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 120

<210> 219

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 219

tcaccactca ctcacaactt cagtctgaag taccatttgt aacacagcaa aaccgtgtcc 60

ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 120

<210> 220

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 220

caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 60

tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 120

<210> 221

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 221

aagtacagaa caagtggact tcaaaagtcc aaagcatttg attaaaaaaa aaaaaaaaaa 60

aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 120

<210> 222

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 222

cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 60

ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 120

<210> 223

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 223

gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 60

gcccagggag cgaagggaga tgttaagtga gatttattac ctttcccttt tagttttttt 120

<210> 224

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 224

aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 60

ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 120

<210> 225

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 225

ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 60

acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 120

<210> 226

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 226

gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 60

atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 120

<210> 227

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 227

tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 60

ggaggcccaa cttctaggac tgacctattt ctgccacaag ctggcctttc tggacttcac 120

<210> 228

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 228

ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 60

aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 120

<210> 229

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 229

acaatgaatg tctgggagtg agaggaaaaa ggaagaaaat gccattactc ctcgtcctca 60

tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 120

<210> 230

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 230

tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 60

aaacgcttgg tggaaaggct ctggtttcgg gcaatgaaaa ggcaggggtc caaatgcctt 120

<210> 231

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 231

ccgtgagtcc tcacatggat ccacacaaag ccaaggaaca cacagaaggc agagagggaa 60

cccacagaca ccaacacaat accaaaccaa accagcctcc caggccgggt atttttccat 120

<210> 232

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 232

tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 60

gcagcaaaga gaagagaagg gagggccagt atatctctga gacagctaag ctaggggagg 120

<210> 233

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 233

ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 60

cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 120

<210> 234

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 234

ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 60

attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 120

<210> 235

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 235

gtctagaaga gacaacggaa gcaaaatgga caagcacagg acatgagacc tctaagagac 60

gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 120

<210> 236

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 236

attacctttt gccacaggtc tggtgacagt gagccacgca gactggttag cttcaccaat 60

ataattggaa accttacaca catactcccc gctctgggcc tctgtcacat tgaacagggt 120

<210> 237

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 237

catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 60

tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 120

<210> 238

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 238

cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 60

ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 120

<210> 239

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 239

cagcacctcc gcatccgagc tattaatccc cgaatgctgg aacaaaccaa cgacacacca 60

gtcagttggg caaagctgag atgtggccac gcacgttgct cccgtgtaac tttcaggcag 120

<210> 240

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 240

tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 60

ggacataagg caggttgtct gggccaatct tgctcccatt cacctcgatg tgctttagcc 120

<210> 241

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 241

ggctctgggc actagaaata cagtagggtg gcaaggacag tccagtactg gcttccccgc 60

ctgccccagc agcttggggc ctacatcaaa tccccagcac agggtcccat ccatctcccc 120

<210> 242

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 242

gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 60

tgttatctgt ttctttctcc tctgaagagg agtcatcatc atcatcatca tcctccgagg 120

<210> 243

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 243

gggtggagag gagcagctgg tcaggctcag gagcagggcc caggccagga cctggaggtg 60

tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 120

<210> 244

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 244

tatggcttag aatttatata tggcatttgt ttttccaact cttcgtaaga gatgctctta 60

tcatgcaccc catttgccag aaagatcgta cgtaactcag gacacagggc taagactggg 120

<210> 245

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 245

gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 60

agggatgctc ttggccagct tgtgcacagc catctggctg tggaagtcac tcttcttggt 120

<210> 246

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 246

attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 60

tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 120

<210> 247

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 247

tagtcctggg ggatgtggct agatccctac tgagatggag tgtgtgtgcc tgaagcgtga 60

ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 120

<210> 248

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 248

atgaggtcta actctgggac aaagaggtaa acaactttcc cagcttctgg ttccttcctg 60

ttgcgagatc cactggagat ttaggcaatt accttccctg gattaatgga gcattcaggg 120

<210> 249

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 249

tcttgcccat ctgcccaaca cctgtgagca gtgcctggca cttagcagaa caggcaccgt 60

gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 120

<210> 250

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 250

tcctaactcc caaagcacct tccagaatga gcacaggctt ctgagggaac ggtcattctt 60

gcacacacct gtgccatctg taccatttcc tcccgtgggg aagggtgggc acacagggga 120

<210> 251

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 251

ggaatgatcc cattcggggg caactgagcc tgcccacagg aacttgagcc cccgagacag 60

tggtctcctt cccagtagac tggcccacga agactggtgc catgattacc ttcaagatct 120

<210> 252

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 252

gcagagcaag ggaaggagac agaaagaagg gggactagag gaagaaatgc tccctgacat 60

cttctttgca acaaggaaca aacgaaaacc accaggcagc aaaacctgtt agaggaagag 120

<210> 253

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 253

ggtgagaccc tgagggaaga actccggcag acctgagaag acgatggctg gttacgaccc 60

ttcaccagat gctggcagcc actggcccat ccgagcatgc gggcggtgag cggcatggag 120

<210> 254

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 254

aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 60

aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 120

<210> 255

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 255

gttctcttct ctaccctgca tctctgcact tctaagcagc ccttggggac agttcaaatt 60

catgctattg gctgcacatt ctgtccaaat aggacttcct cccaatccat gtcccaggag 120

<210> 256

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 256

caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 60

gcatacacag ccagcagatg cgagtatgca agggaatgcc taaccccttc tctctctttt 120

<210> 257

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 257

gatgcactgg agtcagcaga cacctgcaag gaagagtggg gtcaccctag agcaaggagg 60

ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 120

<210> 258

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 258

ccgggcccct cctccctgct cagggaggtg cgtgcacgca gtggggacgg cctgagctct 60

ggctctggca cgggcagcct tacctggttg gaggtcaagg ccacgatgcg gtccaggtct 120

<210> 259

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 259

ccttgtgtag ctgaccccaa agccccagtg acgttgctct caaagcttat tacagaccag 60

caccacccat ccctgcagcc ctctgttccc agctcacctc cactttgtga cctctgttac 120

<210> 260

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 260

tcacctttaa aataagcaga ccaaatgcac ctcttccagc ttgacatttt ataactgatc 60

attgcagtaa caactctgtt ctcctgactc tttgcaaata ctttgcctta gcttatggat 120

<210> 261

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 261

aaatggggcc ggcgggcagg ggctgtttgg tttcaggtaa agatagccag aaggtgaaca 60

caggagggat ggcgggggct gcagacacac acatgcacac gcatacacac acgcacatgt 120

<210> 262

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 262

tccggaggac ttgcagtgtg atatggaggg tgcaggaaat tcacgggcta cactagcttg 60

aaacagactc tagagcactt agttcatgat attccactgt gccttgccca gaagccagaa 120

<210> 263

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 263

aaaagaatca ctgcttcaaa tggaatgttc tatttgtgtt taacccactc cctccaggac 60

tggagcaggg gttgtgagtg gagacagtgg ccaatgacct acagagcagt tttagcacca 120

<210> 264

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 264

ggggacgggg tgactccttc ccattcttag tcagggcttc ccaacaatgg cagaggcaca 60

tgtctgtgta tctgatgttc tttcctcaac acagagtggt ccaaagacca tggtaggcca 120

<210> 265

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 265

gaccccatgt gccctcctct tcctcccctt tcagccttcc cctcccctct taaacccaat 60

gcccagaccc aaagggcagt aagataggaa acagtgtctc acgcatacgg tttggtttgg 120

<210> 266

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 266

aataaggccc aaagctataa attagggaca atggagaggg cagggcatta gaggcccaga 60

gagagaggtg gtgctgagtg tgcaaatccc ccatctactt tctgttacct gtctgcgcag 120

<210> 267

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 267

aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 60

taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 120

<210> 268

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 268

gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 60

aagaatgtac gtccagcctg ccccggagct ggaggatccc ctccaagcct aaaaggttgt 120

<210> 269

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 269

gcctatgccc ctccccctgc cgtccccggc catcctgccc cccagagtgc tgaggtgtgg 60

ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 120

<210> 270

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 270

taatagttgg aggtgattcc agtgaagata ttttatttcc tttgtccttt ttcaggagaa 60

ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 120

<210> 271

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 271

gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 60

tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 120

<210> 272

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 272

tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 60

ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 120

<210> 273

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 273

gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 60

tggcaccgca gttttgtttt aaaactggac ctgtatattt gtaaagctat ttatgggccc 120

<210> 274

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 274

agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 60

gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 120

<210> 275

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 275

gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 60

gcagtactcc ccgggtggcc aggacacccc cagctccagc tcctcagggg acgactccgt 120

<210> 276

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 276

catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 60

ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 120

<210> 277

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 277

ttagatttct ataggatttt tctttaggag atttattttt tggacttcaa agcaagctgg 60

tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 120

<210> 278

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 278

tgaggggtcc ctagcagccc accctgctgc tggtgcacag ccactccccg gcatgagact 60

cagtgcagat ggagagacag ctacacagag ctttggtctg tgtgtgtgtg tgtgcgtgtg 120

<210> 279

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 279

atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 60

gcaatgcttc tagagtttta tagcctggac tgctaccttt caaagcttgg agggaagccg 120

<210> 280

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 280

aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 60

atatatataa tttattgagt ttttacaaga tgtatttgtt gtagacttaa cacttcttac 120

<210> 281

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 281

tgaattcagt tggttcgttc tgtactgtta ctgggccctg agtctgggca gctgtccctt 60

gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 120

<210> 282

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 282

ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 60

catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 120

<210> 283

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 283

agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 60

aataaagaca cctggttgct aacctggccc tgtgctttct gtctccagtt ctgggatagg 120

<210> 284

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 284

gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 60

acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 120

<210> 285

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 285

tattttcata caaattcttc taattgctgt gtgtcccagg cagggagacg gtttccaggg 60

aggggccggc cctgtgtgca ggttccgatg ttattagatg ttacaagttt atatatatct 120

<210> 286

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 286

acggaggcct gcgaccctgg gggcacagga ggcaggcatg gccctgggcg gggcgtgggg 60

gggcgtggag ggaggcccca gggggtctca cccatgcaag cagaggacca gggccttttc 120

<210> 287

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 287

gaggggcctt tgttctgggg ggacccagtg cagaatgtaa gtgggcccac ccggtgggac 60

ccccgtgggg cagggagctg ggcccgacat ggctccggcc tctgcctttg caccacggga 120

<210> 288

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 288

tgtgtgtgtg tgtgcacatc cgcgtgtgcc tgtgtgcgtg cgcatcttgc ctccaggtgc 60

agaggtaccc tgggtgtccc cgctgctgtg caacggtctc ctgactggtg ctgcagcacc 120

<210> 289

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 289

ctggcactct tgttcccaca ccccaacact tccagcattt agctggccac atggcggaga 60

gttttaattt ttaacttatt gacaaccgag aaggtttatc ccgccgatag agggacggcc 120

<210> 290

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 290

ccacttccca ccctgcccct cagagactga aattacgggt acctgaagat gggagccttt 60

accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 120

<210> 291

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 291

accttttatg caaaaggttt attccggaaa ctagtgtaca tttctataaa tagatgctgt 60

gtatatggta tatatacata tatatatata acatatatgg aagaggaaaa ggctggtaca 120

<210> 292

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 292

ggcgggcctt ctggggcaca gcctgggcac agaggtggct gtgcgaagag gggctcggtg 60

gcacagcgct caccccgcct cccgccagca ggagtacctg gacctgtcgg cgcctttcga 120

<210> 293

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 293

catcacaggg tgggcctcgg cccctcccac acccaaagct gagcctgcag ggaagcccca 60

catgtccagc accttgtgcc tggggtgtta gtggcaccgc ctccccacct ccaggctttc 120

<210> 294

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 294

gcttgcctgc agggccatgg ctcagggtgg tctcttcttg gggcccagtg catggtggcc 60

agaggtgtca cccaaaccgg caggtgcgat tttgttaacc cagcgacgaa ctttccgaaa 120

<210> 295

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 295

caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 60

cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 120

<210> 296

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 296

tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 60

aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 120

<210> 297

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 297

gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 60

ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 120

<210> 298

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 298

agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 60

tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 120

<210> 299

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 299

agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 60

agcggggtgc gaggagagcg acggaggctg gtggtccccg agcgggcctc ctgcgccagg 120

<210> 300

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 300

ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 60

ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 120

<210> 301

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 301

atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 60

atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 120

<210> 302

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 302

tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 60

aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 120

<210> 303

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 303

ctgttggagg agtctgctca cagctccgta tttctgctgg gctggaggtg agcagtctcc 60

caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 120

<210> 304

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 304

cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 60

aaggggcttc tagaaggaag ttcttacctc attggttgtg agagtgagaa ttcgatccaa 120

<210> 305

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 305

caccctcact gtcgctcttg ggaagaaagg agaccagcaa aatcaaaaga atcccaggaa 60

ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 120

<210> 306

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 306

atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 60

cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 120

<210> 307

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 307

caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 60

gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 120

<210> 308

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 308

actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 60

cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 120

<210> 309

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 309

cagcctcagt gttatcagaa aagagcccat ggaatgttgg caactagaca tttttaaaga 60

cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 120

<210> 310

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 310

agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 60

gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 120

<210> 311

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 311

aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 60

ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 120

<210> 312

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 312

ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 60

gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 120

<210> 313

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 313

tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 60

taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 120

<210> 314

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 314

aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 60

ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 120

<210> 315

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 315

gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 60

tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 120

<210> 316

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 316

atctgcatat tgaaggagcc actgacatgg ctaaatttgc acagtgagtg acagacgagt 60

gtttattttg ttggcaagga tcataaatta ttattatttt ctttttgaga tgaagtcttg 120

<210> 317

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 317

tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 60

aagaaggggc tcatcaatct caggcttcag attctgtgaa ccacaggaat gaaccacatt 120

<210> 318

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 318

gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 60

aacgcgccca gtcagcggga atgcgcccgc cagggtttga cagggagggg ggcgtcaacg 120

<210> 319

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 319

ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 60

acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 120

<210> 320

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 320

ccgctagctg gcactagaaa tttgcatttc agccatttcc aggtccaaac cccacggcag 60

ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 120

<210> 321

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 321

cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 60

ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 120

<210> 322

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 322

gtgcccacag acgactttgt atggcctgag ggctaaagat ggtctttaac attggtaagc 60

ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 120

<210> 323

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 323

agttgccgac ccctgttctg aagctatgaa aagctttcta aattgggaac ttgtgggtct 60

ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 120

<210> 324

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 324

cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 60

gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 120

<210> 325

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 325

gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 60

aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 120

<210> 326

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 326

agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 60

gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 120

<210> 327

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 327

ctctgcctcc actgggctgg cacagtgggg cagttctgct cacacatcca gaagccaaca 60

actattctat gacatgtggt tttgctgaga gggggcaaaa agtgcagacc acagcacaaa 120

<210> 328

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 328

ttctaagact gggggtggca gggaggcctt taatacaaaa atctctaaac tctacatttt 60

ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 120

<210> 329

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 329

aatcttcccc aatgccaaat cactccagcc caagtgggat aaaaccattt ttcagcctct 60

caaatgtgcg caaacagagt tctttttcat tatctgctaa atccttccat cctcacctca 120

<210> 330

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 330

atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 60

agggtaggtt ttattgaatt gggagaaagg aaggagggtc tggagaacgg tctgcaaggg 120

<210> 331

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 331

acaggcatga gccactgcgc ctgaccaact tttcccagtt tctcaatgaa gccataaact 60

ttcagatctg ataggaaaaa aacagggata tcagtagatt ccaagtctac agttaaagga 120

<210> 332

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 332

tccaagtatt cctgaaagaa gggaagagag acgttttatt tcatcttggg tcaggataac 60

aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 120

<210> 333

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 333

tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 60

ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 120

<210> 334

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 334

tccgtggtcc gcgagggtcg gagccagcgc cacgcagggc cctcggcttt tttccccagc 60

gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 120

<210> 335

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 335

caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 60

gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 120

<210> 336

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 336

agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 60

agagtataag ttagaagagg acactcaact taatattctg ttcacccatt tgatccaagg 120

<210> 337

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 337

cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 60

cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 120

<210> 338

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 338

ccccctcccc aaaactctgg aaggcggaga cctaaggcga caggtctgca cagttcctca 60

caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 120

<210> 339

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 339

gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 60

ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 120

<210> 340

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 340

gagcagaagc aacgtgacct tagtttcaag ggcaactaca aaagcaagtt aaaaatgctc 60

gggttggcat aaccaacctc gttcccttct cttagtccaa ctgggcttta gaaagctaaa 120

<210> 341

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 341

tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 60

tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 120

<210> 342

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 342

tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 60

catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 120

<210> 343

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 343

atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 60

agaacacatc tatttacata ttgtctgtgg ctactttgag gctacagtgg cgaagttgca 120

<210> 344

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 344

gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 60

tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 120

<210> 345

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 345

tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 60

aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 120

<210> 346

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 346

ggctacagaa aattcccacc agtttacact tgtcaggatt cattttcaaa tggagaagtg 60

tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 120

<210> 347

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 347

aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 60

agtgctggtg ctgcaaaaag ccctctggat caccccaggg tgacacttcc tccgacttgg 120

<210> 348

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 348

agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 60

atagcgtttc acaccaaaca cataatgggt gccctgagtt tgaaagcagg tttgccttta 120

<210> 349

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 349

ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 60

caggcaccgg agccgtcgcc tccccagcag tgccacccct ggccatcgcc acccctacgg 120

<210> 350

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 350

aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 60

cagaagctgg tgttagaaaa cttcattgta ttccatcaag caaatgatga cgaacattat 120

<210> 351

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 351

ccctttaagt agttgagact atgaatattt attttctgaa ttacatttta ctattgtagt 60

ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 120

<210> 352

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 352

cttttttatt ttagggtact agatacaaat ttcagtatca acggacccac tctacatgta 60

aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 120

<210> 353

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 353

ttatgtaccc actcgatgtt tcaagttatg tcataggatt tcaacactgc tggagcctcc 60

ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 120

<210> 354

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 354

aggggaattt tctacctcta acacgacttc aggaacaatc ctaagtcctc gcagaggttt 60

caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 120

<210> 355

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 355

gtcctccaca atgctagtcc ttccgcgccc cccgcccgga ggacggtgca cccggcgtcc 60

cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 120

<210> 356

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 356

catagttgaa ctatctgacc ctatggaaaa agaataaaca agaccacaga ctccaaccaa 60

cagccaacag gggagtgtgt gtgtaaaaca ctacgcatgt ctcacaagac aaccaaggac 120

<210> 357

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 357

tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 60

tgcttcaaat gcgagcccca gcaaaatgaa aaccggtaac atgttgtggg tccccagacc 120

<210> 358

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 358

ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 60

atgattttca gaaggaaaga aacttcgatt tttatatcca ccagacaata tggcattctg 120

<210> 359

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 359

ccagaagcag atgtcagcat ctggatgggg gtagcagagg cagaacttcc agaacgccgg 60

ccgtcagcac gcacccacct cccccatcac ctatgcagcc acagagggag gacacggagc 120

<210> 360

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 360

tactcatgta tgttaaatcc ataatgcact gttaaaggaa acttcaggaa aatatcggtt 60

aagtttcaaa gttttgaagg ttcgagattc agaaagtgtc ctttgctccc caaggcatcc 120

<210> 361

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 361

agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 60

cttcagtgag gcaatgtgtg ctctcttcta ctatccacta tcgcctccac cgccctcaat 120

<210> 362

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 362

ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 60

tacatgtggg aggaaagtac acaaacattc tcattgattt actaagcatt tgcagaatgc 120

<210> 363

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 363

aactattgac aggtagagaa caataaaacc tagttgagct ataggtgctg ggcaggcaac 60

tcggcttcac acggacctaa ataacccgct gagtctacac cacctcctca ctagagccct 120

<210> 364

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 364

accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 60

ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 120

<210> 365

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 365

cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 60

tttcatgagt cagaactcac aaaatgaagt tctgagaaaa atcatgattt gagaggaacc 120

<210> 366

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 366

tctttcagtc ttccgccaag cagccactta cgaagagtga acacaactgc agcgcattca 60

acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 120

<210> 367

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 367

ggcctgtgct gggctcttca atttaccatg acattcccaa aacccccgcc ctccaagaag 60

gcagaccaat acagtgccct ccgctttggg cttccaccta aggggtgatc tgtccactgc 120

<210> 368

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 368

tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 60

aaagaggctg caaggcatat agcattacca acaggatcac ataggatagt actgtggtaa 120

<210> 369

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 369

cgggctcccg tggcgcccac gtccccggct ggcgaagctc cccgaggcgt cttgcgcggc 60

gattaccttg aatggcaacg ctcctccgcg acctgtgttg tccccgcgcc ccgcgtgggt 120

<210> 370

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 370

ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 60

ctagccccag gcgcactgcg ctccctccag cgccgacggc ggctgcgggc gaggggcgcc 120

<210> 371

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 371

gagtcccagg tgataccacc agaccggtcc acagaaatgt gtgaggctaa tctttttaaa 60

agcatacttt taagaaacag agataaaaca gtagctacag gactcagata cgtgcagcca 120

<210> 372

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 372

taatgcttgc gtccaatcag aacttgcgag cagcttgggg gtgctcccca ccaccacccc 60

ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 120

<210> 373

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 373

atggtgtgtg cgtgcacggt gtgtgcatgt gctttcagat agcttgaaag atttttcctc 60

ttaacttttc tttcctctta gtcaagagcc tcagaagata atttcggatc tatttctaca 120

<210> 374

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 374

tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 60

cccaaagaca ggcagagtga caagcaggaa gggcttggct gcggagcggt ccccatgcca 120

<210> 375

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 375

aaattaagtg tattattttc cttctaaaat acttggggga ctttaacttt tttcttgttg 60

agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 120

<210> 376

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 376

cacacacaca cacacacaca cacacacaca caaaaccctc taattcatca ccagggttgg 60

caagagaaag agtccccatc ccagcatccc agaaggggaa tgtatcagtt ccaacaacat 120

<210> 377

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 377

aggggcgcct gattgctttt ctattcaaac taaacaggct tttcagatga aaatgagtcg 60

agcccggtgg aagatttaca gggatttggg accagggaga caatggagtt ttaaatgttg 120

<210> 378

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 378

atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 60

gcctgcgggg ctgtgccacg ggcaggcgaa ccttccgccc gtctgctctg gggtcggaga 120

<210> 379

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 379

aaggtgaata cggttcgaga ggctgactga ggtccaagta ttcctgaaag aagggaagag 60

aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 120

<210> 380

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 380

cagcattaac cacacaactg gaaacaaaca cgcccaccac atcaaaagag aactttttgc 60

ttacaatgat aaaaacgaaa ttttgtccta aatggaaccg tttttcttga gcatatggta 120

<210> 381

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 381

gcggccccgt ggccgaaaaa aatgagcgcg caagttagaa ctcgcctgcg ctttcgacat 60

ctcccagccg ctgcggcaga gcaggcaggc gaactagaaa aagtcggtcc agttcgcccc 120

<210> 382

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 382

caccccaggc tccttccccg gcctgctcgc ccacctgtcc ctgcccagag gagcctcctt 60

ggctccaggc agagaaaggt ggacagggcg ctccgctgaa tctcaagccg ttccatcatc 120

<210> 383

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 383

gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 60

agcacaggtg aaacggtgtg attcacagca aaagcaagaa aagtatgtgt gcagtgtgtg 120

<210> 384

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 384

agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 60

aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 120

<210> 385

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 385

ctttcagggc agcacttctt tcatttgttt gcctgaaata tcctgttttt ctcccccttc 60

tcggggcgct aaagagctca gagaaatctg cattttaatt gacattccag ggaaagagag 120

<210> 386

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 386

ggttgaaaaa aaaaatcaaa agaagaagaa tatcttatag gattaaaatg aataaaattt 60

atatacaatt atataaaatt ccaatttcag gatccataaa attttactag aagacagact 120

<210> 387

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 387

aagagtttta tttcaaacac aaactcttga gatgtgggta ttggacgtgg gacaggacag 60

gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 120

<210> 388

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 388

ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 60

tggatggctc ttctcggccg cccgcccccc ggtccatttt cacctcggca caagcctgcc 120

<210> 389

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 389

ttcggggata attgaagggg aatacttctt cattcagtgg gtaatttaac agatgtaact 60

gattactctc agaagctctc cgagttgaaa atgtaaacag gttcaaaaaa ggcttagaca 120

<210> 390

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 390

tggctcggcc actgggatca cctggtgttt tgttcctggg tagatttcac atcaggaaag 60

tcaacagtgt ccggctcctc caaagtgcag acccatctct tcgcttaagg cctccctact 120

<210> 391

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 391

ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 60

atgggtttat catctttcct gctcagagca tatgctttcc ataattataa aaggcactct 120

<210> 392

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 392

ctttatccag cacaggccac agtcagacgc ataagataaa gctgctgagc taagacttca 60

tatcactaca tttgattatt actttttaaa aaatcactct aaaactattt cctaaatcag 120

<210> 393

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 393

caccagtgga caaaagtatg gtgcgacctc ctaggcagga taattaggtc cccaggctcg 60

gtgttgggaa tacaggccca gcatgccgct ggcggccaca atcccggctt cctgccttca 120

<210> 394

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 394

tctccccagc actgagtagg agcgggagct gtcaaggggt caaggctagg gggaaattcc 60

tcaacaaatt gaaatctcaa aaaacacaca ctgacccagg accacaaagc cctcccggtt 120

<210> 395

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 395

ataccataat ccttcctgga gtctgagccc cagtgaaagc cccagcgtgt ttatttttcc 60

ccttgaaagg gaactctctt ctcagcctgc acgcgggagg agttgtcacc ctccctctga 120

<210> 396

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 396

acgcagctga tccaaaaagg acttcgaagc ctgaattccc ttcccctgtg actaaatggc 60

agtccagggc tgctaaatgt cttcaacctc tgtagaaggg tgaggaaact gagcagatgc 120

<210> 397

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 397

acttatacta gaaacaacaa ttttggcaga agaagaaagt tggtttcttc ccccccttga 60

accgttcctt tcctttcaac ttctgaaggt cagctgtatt catttaagtg caatctcttc 120

<210> 398

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 398

agacaattgc atggtttgga gttagtttcc tgtgtaaaag aacactattg tgcatcctca 60

ttaaatatct caaaatacat tcattaacaa tttctctatc ttaagatttg actagacttg 120

<210> 399

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 399

cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 60

cggactgctc caccagcagc tacactgaag gcagcctcgg ggatgtccct ctgtctttgg 120

<210> 400

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 400

aaattctgat gaataatgca ggaatttatt aataatgatg atgtaaatta tgtaaattat 60

agtcatggta tgtgattgga ccaataactt tccatatgca aacctactgg ctaaattttc 120

<210> 401

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 401

ccaaatctca tcataaaaac tgctcctttt agctttgact gaggacaaaa atggaggagg 60

gaaaagaata tgccacatca tttccaaagg aggaataaag aaaacctctg cttcaaagat 120

<210> 402

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 402

aatatataaa taaatcactt tgagcataga catgagatca ttaaaataga actttagtgc 60

ttttcccggt ttctttcttt tttattttta tttttttttt ttttgagacg gagtctcgct 120

<210> 403

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 403

gatgtcaccg gccccacccc agcggccccc tcccgaaaaa ggggtctccg cagggcctgg 60

atggtacgga agttcccagg acacacggat ggggcacctc ggagatgacc ccgggaaccg 120

<210> 404

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 404

gaaagagaat tttccaagaa agcttaatat attccaagca actttaaatt cactcagtac 60

tctctgtgac taagggtaca gtaaaatgca caaactttag agaatgatta aaacaatcac 120

<210> 405

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 405

acaggcgaga gcataaatat tttagggaaa ataaatattt taacaaaagg tacatgtttt 60

tatgtaaaaa tcatgtaagt tgctcattgt agaaaacagt gtatgtttat atacacctag 120

<210> 406

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 406

gagaccccta gaaggtgaac agagacccag cccacctgac ttccacgaag acctccctga 60

gaccacgtct gatgtacccc aggctgtcct tagcttttct cttggaatcc atcataaatc 120

<210> 407

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 407

cagtttaccc actaatgacc aaagcacaga atcaaacttg cacaggaatc caagcttttt 60

cccatgcaca ccaaaatgca agtgaatact gcttcccctt tcccactctc ccacataccc 120

<210> 408

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 408

gatgctccag gtggcataac ggacctgagg ggaaatgcca aagggataca ttgagggtcc 60

agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 120

<210> 409

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 409

agaggaaaat gcaggcccca tgacaatgtc ggcaccccgt ggcacctgcc ctccatatca 60

gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 120

<210> 410

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 410

gagcctggtg gcacagggcc ccaggctgca gggttggcta ggacaaggcg tggattgccc 60

ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 120

<210> 411

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 411

ccctaccagc ccgttcacac tctgcaagct ggcagatggg gtgtaaagac tccagaagtc 60

tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 120

<210> 412

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 412

tccactgtga cgttcaagat cattcgtgat ccggacagat gtgccttctg caaacactcc 60

caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 120

<210> 413

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 413

caaatacacc aagaatgttc ccaactgtgc acctctccta ttacactaag aatccagccc 60

ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 120

<210> 414

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 414

ccactgtcct tgacagctca tttcaaaaac catctcctcc agtccagtct ctggtcagaa 60

ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 120

<210> 415

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 415

ctgtgctcac cctttcctct ctgcctacca aagcactgat cacagtctac tttttacggt 60

agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 120

<210> 416

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 416

agctgctcat ggacatttgc ctcctatcag actgcaaaat ttttatgggc aggaacgcta 60

tcttgcagca tttaaagcat ggtgtcttgc atgtagtgag tgcatggtga attaatttaa 120

<210> 417

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 417

tgcttacacc ctttaagtta gtaatttcac ttgtaacaat ctgtcctaag gaagcacgct 60

taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 120

<210> 418

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 418

taaaaaaatg ctgacagaga ttcactgcaa cattatttat tatggtgaaa aaccagaaaa 60

tacctaatat cacaaaatag ttcattaaat tataggactt tttctgttgt tttttggaga 120

<210> 419

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 419

tggaatctta tacagtcatg aaaaattatg tttcaaatat tcatgcttga aatatatcag 60

gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 120

<210> 420

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 420

gaaaagactc atgttaacaa atgacaaggc aaggaaatta catacatgat gtgatcaact 60

atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 120

<210> 421

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 421

atgtaaaaac catattacag aaagaagact gaagaaatgt ttaaacttta tttgcatttt 60

tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 120

<210> 422

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 422

tttgtattta aaaattttaa tgaataacct gtataggtgg aaagtattac ttaaaaaaat 60

gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 120

<210> 423

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 423

gaaaagcatg taatcaggac ttcctaactc ggcctcccct gttcccatta ctctaacttt 60

cgcatgcaca cacacgtacc ttgtagcctc caattctgtg gtcaggtttg aattctttgc 120

<210> 424

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 424

agggctagaa gcgcgacggc ggtagcagct aggcttggcc cccggcgtgg agcagacgcg 60

gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 120

<210> 425

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 425

gacccctcct tcctggcggc ggcggcgcgg gctcagagcc cggcaacggg cgggcgggca 60

ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 120

<210> 426

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 426

ggtaggagag cggctacacg gcgcggggag gctggggtcc tgggggcgcg aggccgtccc 60

gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 120

<210> 427

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 427

gccccgcccc gccctcggcc ctcgccctcc cgccccggcc gccgctttcc ccgggaagcg 60

tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 120

<210> 428

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 428

tcccccacgg ctcctcctgg ggccgccgcc agaacctgta ccgcgccctg tcatgggacc 60

cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 120

<210> 429

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 429

cggccctaga ccgaccgccg ggctgggccc cctggatggt ggccaggggc ggctagagct 60

gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 120

<210> 430

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 430

gggccaagac cgacggcgga gcgggcgccc cgaggtgctt tgctctcctc gaccctctcc 60

tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 120

<210> 431

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 431

tgtctctcgg tcgcccacct tggctctttg ttggctccca gcacgcggac cctcctgttg 60

ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 120

<210> 432

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 432

ttctttggtc ccttccggcc tccgtcctgg ggccaactcc atctaggccg cctgcggcgg 60

acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 120

<210> 433

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 433

acacctgggc ccctaccgct tccccgcacc tgctgcgccg ctccaccttc cccccgcacc 60

gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 120

<210> 434

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 434

gcatccctct tgtgcaccag agtagcacca aagcaccagc ctggggccgg tcccttctct 60

gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 120

<210> 435

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 435

gcccagagcc cctcccgggc ctccatagtc agtaaaggcc cctcctgact aaggctgggt 60

ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 120

<210> 436

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 436

ctgctgctct cccgagctcc tccaggccgc cagaaggctt cctccgagtc actttctccc 60

tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 120

<210> 437

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 437

tgagttcgag gcttgcgggt cccccagcga tccagcaccc cttcccctgc cctgtaccca 60

gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 120

<210> 438

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 438

gttattcagg gcgaaaacct aagcgtcgtc cgtaagtccc atatgtgcca gtggccaggc 60

ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 120

<210> 439

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 439

ccgggtctgg tctggcatct actccttggg ctccagaagc ctgtggtttc cctgcttccc 60

ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 120

<210> 440

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 440

ttctgctctt aacctcccca cccacacaca gcaccacagt gggcttctca agctgcagca 60

ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 120

<210> 441

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 441

ccgacctgcc ggggctgccc cctctctctt ctccaggacc ctccccaact ttgctcttct 60

tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 120

<210> 442

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 442

tcaaatcaaa gcctccccag gtccccctcc accaccaggt gcctgtgggg ttcctgccct 60

ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 120

<210> 443

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 443

ggtcgacact cggttctctc tggcagcctt tttccctacg tcattcccta gactgggaac 60

gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 120

<210> 444

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 444

gctgtagaca gaaagggtgg caagtgtgtg ggtgaccggc gtgaggacct gggcagccct 60

ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 120

<210> 445

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 445

ggaacctggg actgactgga ggctctgatg tggaagttcc ttggcacctg ttgctcagct 60

ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 120

<210> 446

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 446

ctgggcttcc cttgtttgcg catcgtctcc cagctcctcc tctgggcctt caccctctca 60

gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 120

<210> 447

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 447

gcctggtctt gtcccgctgc ctagcccggt gggagagagc agaccagagc cgctgtcctg 60

cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 120

<210> 448

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 448

cttttctgcc ctctactgcc cggtcctgcc tcacgtcaca cgcagaggcc tgtggggaga 60

ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 120

<210> 449

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 449

ccagggcttc ctctggctca tccgggtttc ttattggccc gggcttttcc gtctcttgac 60

ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 120

<210> 450

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 450

ttctgcttga tatagtaata cagcagtaga gattatgcag gcttgggttt tctttctcac 60

catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 120

<210> 451

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 451

catctcagcc tgaaggccct ttagctcttg gtcccaggaa gcagcagtgc tgcccatggc 60

tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 120

<210> 452

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 452

tccccctgcc aggctggaga gtgagtctct gaagcaagat ggggactcag tctgaggctt 60

tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 120

<210> 453

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 453

tctctgccaa gagacacgtg tgggaggtgt gagttccaga gcaatgagca cacctgtgtg 60

ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 120

<210> 454

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 454

ggaagtggtc gtgccccttg cagcagctgt cacgtctgtg tctggacaat gtggtagtgt 60

tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 120

<210> 455

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 455

tgccctcaca ctgacacaaa catgttctgc ttttccagaa tgagtctgca ggtcttaaac 60

gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 120

<210> 456

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 456

gacaaaaatg tcagcaatga aaaaaataca gaaaattgcg acttcctgtt ttcgccacca 60

gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 120

<210> 457

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 457

gaagttaccg gaagatcgtc tgttcttcgt gtgtcacaga aagaaaatgt gccacccaag 60

aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 120

<210> 458

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 458

aacctggcca aagctatgaa ggtaagtgtg acctgtacag tgtgtggctg gccaggttgc 60

cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 120

<210> 459

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 459

cctagggcct gctttcttgg tgacctctga gctgcaggac actgctggtg tggctgtaac 60

agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 120

<210> 460

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 460

agcttgaact aatgaatagg tgctctcctc ctcactccgc aacaggtgac ttttcagaca 60

cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 120

<210> 461

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 461

cctctgcggg atccacagac gcacaggatt ctaagtccta gcatggccag caaacttgag 60

gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 120

<210> 462

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 462

gctcctttca ctcaggatga cacccttgga ctggaaaact cacacccggt ctggacacag 60

aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 120

<210> 463

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 463

aaagagaagt aagtgttggt gctgctggac atgctggagc ttcaccctct ctgtccgatg 60

gttcttgcag gaaagtgctg tcttgttcta tcaggccttg gctggatttt ttgtttgttt 120

<210> 464

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 464

aactgcaagt ggatggcgag gcggtgtctg tctttgccta gaaagggagg atcctggtgg 60

cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 120

<210> 465

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 465

cagctcttta ctctgcggct gaactaatct gtctgggcac ccaggaacac ctcatctgca 60

tccagggtgg gaagggaccc cagaggcatg attttctgtt ggttttttgc tgatgtaact 120

<210> 466

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 466

tttaagcaga ttttagatgt gcagagaaaa gcataaggaa cagaatacca ctgatgcttc 60

tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 120

<210> 467

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 467

tcaccccctg agataaaatg tcaccagtaa gtgcaggcag catttcctac atccttctct 60

gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 120

<210> 468

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 468

gggccctggg tccgtctccc ctctacccct ccagcctgaa tttgcagttc ttcccatgtc 60

tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 120

<210> 469

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 469

tgcttgctac ttttcaactt tgttttttgc cccttttgaa atttggtcaa acgaagcgag 60

aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 120

<210> 470

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 470

aatgaatcaa acattggttt acaacgaccc caagaaggaa gggagaccca gcagcagaga 60

agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 120

<210> 471

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 471

agtcgcctcc cagctgccat gggtgtgatc cgtcccagag ccacagatcc cctgcctctc 60

ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 120

<210> 472

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 472

ccctatgcct ccaggatcac ccagcagcct ccatagggca gagggtgtag aggagctggg 60

aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 120

<210> 473

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 473

aggggcttga gaggttggcc ctgaggggct ccagccagag tgactctgag cttgtggctt 60

ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 120

<210> 474

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 474

ggactcgtgg ctgtcatctc ctttctggaa ctctgctgag gcccaggtgg tggtcactgt 60

ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 120

<210> 475

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 475

ggtgctccca cgtcagtggg atgagaccaa gagcagccgc tgcgtgcagc tgcccacgtg 60

tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 120

<210> 476

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 476

tcattcagag gacagccgag tggccgcctg ggaaggcagt gccgccccaa ggtggcagct 60

gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 120

<210> 477

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 477

gggcagcaga ggagttgtga gcagtctgac aggtccctca ccctcagttt ctcagttttg 60

acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 120

<210> 478

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 478

acatcttcaa acttagagtt ggaagagaag ggcaggtgac atgggcccct tatctgaggc 60

actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 120

<210> 479

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 479

actggcacat gaccctgcgt cttctcaggg acctgaaggc tggggaacag gtgaaacagt 60

gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 120

<210> 480

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 480

gtttgtgccc tctgtgttgt agtccagccc caaccatccc aacagccgcc tcttctgtca 60

ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 120

<210> 481

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 481

ggggccctgc gccatgcagt cccacattgt gagcagcgag tgccccagag acaggtgggt 60

ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 120

<210> 482

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 482

ccctgggaag gtgttctctg cacctgtgcc actccactgt agcaggcgcc atcccaggga 60

ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 120

<210> 483

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 483

ggcgtcgcag aggccactca ggtccttctg tgcctgcagg tggcatcctg cacctcagcc 60

ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 120

<210> 484

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 484

ctgcccttca gtgaaggctt tgactgtccc tggccacagc actgccagtc cccgacttga 60

aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 120

<210> 485

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 485

aggggaaagc agattcatgc agaaaacctt tgtttaccac gccccctgct ggccatcctg 60

gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 120

<210> 486

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 486

gggcctgcaa gtccccagga ctgatctgtt gggcatgtcc tcgggtgagg tttaagccac 60

aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 120

<210> 487

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 487

aggacaatgt tgactgagct ttcttgtttt gcttgtagct gatgttttca cgcagattaa 60

agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 120

<210> 488

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 488

agtgttatcg gaatgctgtc aggtccctta atgttagaat tcagggtcat taagtggtgc 60

acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 120

<210> 489

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 489

acacccttca gtgattagga gaaacacagg cggaaataat gattatatga cttacaggcc 60

cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 120

<210> 490

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 490

cacagcaggc tggaggccac acagaggact ggggccaggc cctggagcat aggcagggga 60

cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 120

<210> 491

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 491

cagctgagag ggtggcagcc agccttgtct atcggggagt agggtggggt cactgtatgc 60

tggtgggcag gtgctcgaat ggatttttta caggaaatgg tggatgccgg gtgcggtggc 120

<210> 492

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 492

gcatcagctt gaggcatttg ggtgtgctgt cccacatctg accccccagg cagcagcctg 60

tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 120

<210> 493

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 493

tgctgtgtgc atgctgtggg cctgggggca gacctcgtgc atgatgacga gtgctgtttg 60

ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 120

<210> 494

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 494

ggtggcagag gctgctggag gagtctgggt ccagcacctg gcaaagcgcg tgatgagcac 60

aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 120

<210> 495

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 495

aattaaacag gcaagcagcc cagaggcacc cctgtaactt ggaaccccaa gaagcatgag 60

ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 120

<210> 496

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 496

ggcggggtca gcgaggaagt cacgtggttg tgtccagccg ttccaccggg agctggtccc 60

tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 120

<210> 497

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 497

tgggaggaca ggaaggaact acattgagga actgaggtgg ggggtggaga acgggaagcc 60

gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 120

<210> 498

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 498

gtgccaaggg tgactcagcc ctgcttctgg tgtgagaatg ttaaacctat gttgagaatg 60

aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 120

<210> 499

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 499

aatgctgagg cccctaacct caccaggtac tcgctgcttc acgttgatta agtctctttt 60

aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 120

<210> 500

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 500

aaaagccaac agctcatcaa ggaagtggat gccaaaacta ctcatggaat tctacagaaa 60

ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 120

<210> 501

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 501

ccagtggagg ctgacaccga cctcctgggg gatgcaagcc cagcctttgg gagtggcagc 60

tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 120

<210> 502

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 502

tccagcgagt ctggcccagg tgccctggct gacctggact gctcaagctc ttcccagagc 60

ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 120

<210> 503

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 503

ccaggaagtt ctgagaacca aatggtgtct ccaggaaaag tgtctggcag ccctgagcaa 60

gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 120

<210> 504

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 504

gccgtggagg aaaaccttag ttcctattcc ttagacagaa gagtgacacc cgcctctgag 60

accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 120

<210> 505

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 505

accctagaag acccttgcag gacagagtcc cagcacaaag cggagactcc gcacggagcc 60

gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 120

<210> 506

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 506

gaggaagaat gcaaagcgga gactccgcac ggagccgagg aggaatgccg gcacggtggg 60

gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 120

<210> 507

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 507

gtctgtgctc ccgcagcagt ggccacttcg cctcctggtg caatccctaa ggaagcctgc 60

ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 120

<210> 508

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 508

ggaggagcac ccctgcaggg tctgcctggc gaagccctgg gctgccctgc gggtgtgggc 60

acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 120

<210> 509

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 509

acccccgtgc cagcagatgg cactcagacc cttacctgtg cacacacctc tgctcctgag 60

agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 120

<210> 510

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 510

agcacagccc caaccaacca cctggtggct ggcagggcca tgaccctgag tcctcaggaa 60

gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 120

<210> 511

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 511

gaagtggctg caggccaaat ggccagctcc tcgaggagcg gacctgtaaa actagaattt 60

gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 120

<210> 512

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 512

gatgtatctg atggcgccac cagcaaaagg gcacccccac caaggagact gggagagagg 60

tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 120

<210> 513

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 513

tccggcctca agcctccctt gaggaaagca gcagtgaggc agcaaaaggc cccgcaggag 60

gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 120

<210> 514

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 514

gtggaggagg acgacggtag gagcggagca ggagaggacc cccccatgcc agcttctcgg 60

ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 120

<210> 515

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 515

ggctcttacc acctcgactg ggacaaaatg gatgacccaa acttcatccc gttcggaggt 60

gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 120

<210> 516

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 516

gacaccaagt ctggttgcag tgaggcccag cccccagaaa gccctgagac caggctgggc 60

cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 120

<210> 517

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 517

cagccagcgg ctgaacagtt gcatgctggg cctgccacgg aggagccagg tccctgtctg 60

agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 120

<210> 518

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 518

agccagtaag tgtgaggatg ggatggggag cgtggcgtgg ggcagctgcc ctgcagtgta 60

tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 120

<210> 519

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 519

tgtggtccag gacccgaggc cagaagtgtc atcagatact cctttgaggc cgggtaggtt 60

ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 120

<210> 520

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 520

ctgcctgagg aggggcctct ggataaaaac agggctcagg tggctcccag ctgagggtgc 60

ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 120

<210> 521

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 521

ttggccttgc cttggctgcc atgcatctct ttagtgctcc tggcctaagg agggatccaa 60

taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 120

<210> 522

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 522

taagggcggg ctcaggccag aagccaaatg caaccaaccc tgggcaggac cctgtccccc 60

agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 120

<210> 523

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 523

agtcatgttc ctcctcaggt gaacttgact tgggtgtgta actccaaaag ccaggcaaag 60

cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 120

<210> 524

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 524

cctgttactg cctgggtggc gtccgctgct tgctggggtc ttggccgtgg gtctgctgcc 60

tcctggctga atggtcttag gctacatctc ctagagttgc tcagtgggag attgtcctgc 120

<210> 525

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 525

tgggtggctt gctgcccaca cctgccccag cctccacatt tgaagccttt tgtcagtttc 60

acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 120

<210> 526

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 526

acagtaaaag ggatgtactc atccttgtga ttccctttgg ctgcatcgtg ctgactttag 60

agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 120

<210> 527

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 527

agctcccagc ctgcttctcc cagagctggc aggcaaggcg cgtgttttcc tgctgggtga 60

ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 120

<210> 528

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 528

ggttggtgag gcagttttgt ggccaggtcg cttgggacag ccccatgcct ggcacgctct 60

agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 120

<210> 529

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 529

agctgaatgt tcacgtggcc ggcacagcag tgcagggaaa ggcgatggca gctcagtgct 60

ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 120

<210> 530

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 530

ggagcagggg acgccggggt tatggggtgg ggggcatggg cctctgctga ctctgtctcc 60

ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 120

<210> 531

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 531

ccaggcagct gcattcagcc tcagcggagg acacgcctgt ggtgcagttg gcagccgaga 60

ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 120

<210> 532

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 532

ccccaacagc agagagcaag gtaaggggtg cttgtgtggg tacctgtgct cctggcctgg 60

tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 120

<210> 533

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 533

tcgtgtagaa gagtagcagg cagtggaagc ccccagcagc cttgggtgac ggggtgggat 60

gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 120

<210> 534

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 534

gtcccgctcc ctctccccca agtctacttc aacaaggttg tggggaggca aagccacacc 60

ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 120

<210> 535

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 535

ccaagtacct tgactgcact gcacagggtg actctgccct ttcctccagg agagagcctt 60

gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 120

<210> 536

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 536

gaactctgcc agcacctcgc ttcccacaag ctgtccaggc agtgagccag tgcccaccca 60

tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 120

<210> 537

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 537

tcagcagggg cagcctgcct tggagctgaa agaggagagc ttcagagacc ccgctgaggg 60

tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 120

<210> 538

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 538

tacgttgcct ggcacagacg tcacacacag tctgcccgga ggggatcccg tgagcacttg 60

ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 120

<210> 539

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 539

ggcagctcga gacacctgca tcatcggcag gtggtcattt cgcaggcagt tgggtccctt 60

gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 120

<210> 540

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 540

gactttgaat gactcatgca caaacttgtg gtttgtcaaa acttagtcag tgttaggaga 60

gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 120

<210> 541

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 541

gcagggctca tacctttaac ataaaagact tctagaaatg gaaactgtag actcaggaaa 60

atgagcagga gaggtgaaca atgcagtgcg ctaatgagat acaagtgtag agtcaacaac 120

<210> 542

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 542

tgcagttaaa taaatacggt ttgtccgtaa gattggaggc tgcagttaaa taaatacggt 60

ttgtccgtaa gattggaggc tgcagttaaa taaatacggt ttgtccgtaa gattggaggc 120

<210> 543

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 543

aataaatacg gtttgtccgt aagattggaa ggtggaacgg tccttaaatt aaagtgcatt 60

tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 120

<210> 544

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 544

tggtaggaat tgatggctga gttgttttaa aggacacaca gccaggtggg tataagatct 60

caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 120

<210> 545

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 545

caatttagag aactgaaaac ccaggcacac agcagacacg aatggcatgt agggtttgtc 60

gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 120

<210> 546

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 546

gctgacagga tagttgtggg ccgccaccat gtggggatat cagctcagct gggatgggcc 60

tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 120

<210> 547

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 547

tgtcaccctc acgcaggatc cctggaatca cacagcacgg ccttctgtgt ccggcgtctc 60

cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 120

<210> 548

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 548

cctcggggag gtgtgtttcc gagtcctggc catgctggag caggtgtggg tgctcctgtc 60

cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 120

<210> 549

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 549

cttgccgtgg cagcttggct tcacatcatc ttatccattc attcatggat ttgctggttt 60

ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 120

<210> 550

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 550

ctgcttttta gctgtcagga gtagtgctgc ctcgaatgtt caggtgtaag ttctatgtgg 60

atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 120

<210> 551

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 551

atgtattctt ccatttctcc tggatacata gctaggagtg gagtcgctgg tcacagtaac 60

tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 120

<210> 552

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 552

tctgctcacc ttttagctac caggtggcct tccagagtgg ctgcagtgtt tcacattccc 60

actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 120

<210> 553

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 553

actggcagtt ataagagagc tccagtttct ccacatcctc gccagcgctg cttgtctgtc 60

ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 120

<210> 554

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 554

ttgttgatcc tagacttcca gcgggtggga agcggatctg gttgcggttt tgatttgcag 60

ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 120

<210> 555

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 555

ttcccaggtg gctcatgaac gagcgtcttt ttatgtgctc gttggatatc ttctttggga 60

aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 120

<210> 556

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 556

aagtgtctgt cgagatccct tgtccattga gccacttatg tttttattac tgagttttaa 60

gaggtaaatc ccttttattc aagaagtgag ggaaaggaaa aaatacaaag ttaagtccct 120

<210> 557

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 557

gacaaggcca tagcagagaa ggacctacaa gtttttgcta gactaaataa ttggcaaccg 60

ttttttagct taattgagaa aaggagagaa tgagcaaata ctgctaggca tgaagaaagg 120

<210> 558

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 558

ccacccttag ttttaatcaa aagttatatt atcaaaaatt aaaagacttg tctagggtaa 60

ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 120

<210> 559

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 559

ttttaaagac taatagttct caaccagagc agttttgccc ccgcctttcc ccagatgttt 60

gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 120

<210> 560

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 560

gtggttgtca cacctggagg tgctgctggc agggagtggg tagaggctgg ggatgccgct 60

cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 120

<210> 561

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 561

cagcaccctg cggtgcccag gaggcgcctg ccgcagacag cagtcaggcc ccgaacatcc 60

acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 120

<210> 562

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 562

acacctccaa gggaggaaat actgctggct ggaatgatcc tgcctcactg agtgctgagg 60

gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 120

<210> 563

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 563

gagacaccag cccgccgccc ttagggccct ggtgaggggc gatggcggcg gcatgattca 60

ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 120

<210> 564

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 564

ctcctctcag ttctaggcac gggcgcggag gtggattacc tggagcagtt tggaacttcc 60

tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 120

<210> 565

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 565

tcggtaggta ccaggcaatt ccgcgaagcc tcacccacag ggtgtccgag agcagccacg 60

gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 120

<210> 566

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 566

gcaggtcttg cccccggagc gtcccttggt gcccacgtcc ccccagctgc acacaggccc 60

aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 120

<210> 567

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 567

aggcattgtg gcgggctgtg aatccagggc cccttctgca gcacctcctc taagtggtgt 60

cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 120

<210> 568

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 568

cctgtggcag ggctctaggg ctctctacct ggtgcctagc ccaggatgaa actctgacac 60

gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 120

<210> 569

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 569

gctgtgctgc tgggaatggt ggtgtctcgg gcagggttgt gggtgaccgg gggtgggagt 60

gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 120

<210> 570

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 570

gtgcgggtga ccgggggtgg gagtgtgcag gtgacctccc tggcccttag cccccgtgtg 60

tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 120

<210> 571

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 571

tgttagggga tggcagtcag acctgatcac ttgccctctt gtccccagtt taaggagtcg 60

gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 120

<210> 572

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 572

gccttgagga agcagtcctt atacctcaag ttcgaccccc tcctgaggga cagtcctggt 60

agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 120

<210> 573

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 573

agaccagtgc ccgtggccac cgagaccagc aggtatgtgc gccggccctc cctcatgcat 60

gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 120

<210> 574

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 574

gaagccttga gtgtgggaag actggaggct gttcctaggt gtgctgtctg cacagaggct 60

tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 120

<210> 575

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 575

tctagaccgg ggggagatga tcagaactgg aaaggagctg tgctaggggt gggctgggca 60

gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 120

<210> 576

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 576

gcaaggccag agcagccctt gtgtgtctgg caggagcaac ggcagcctac cctccagcca 60

cagggctgct gccttgctgg ttacagccac cgttctaggt atattaaaga tctaaactta 120

<210> 577

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 577

gcaaactatc acctgtgggc cagacttggc cactgcctgt ttgtgtaaag ttttatcgtc 60

acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 120

<210> 578

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 578

acagagccat gcccgttcct ttgcaaattg tggcaacttt cacactacaa ggccagaact 60

gagtagtgtg acagaggctg tgtggccttc aaatcctaac acatggccgg gcgcagtggc 120

<210> 579

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 579

aacagccccc tgatgaggca gcactggctc cgctcagcct gcagtgggta atgaagcgtc 60

accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 120

<210> 580

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 580

accgtgctct cccggaagga caagcagcca cgtgggatgg gcaggtgagg gaggggggca 60

cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 120

<210> 581

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 581

cggagcagca gagaggccgg gcatgctgga tgcgggtctg cgttttctac cactaccctg 60

acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 120

<210> 582

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 582

acttgagtgg ttttaaacta aaaagcaaag agcccggtta gcttgtccac atggtcctct 60

gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 120

<210> 583

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 583

gtgtcctact caacactggg agggcctagt gactgaggct gcctctgctt ggtggcagca 60

tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 120

<210> 584

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 584

tgcacggtgc aaatgagact ccctcaggac gtccgcggga agccaagctt gtggagttcg 60

atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 120

<210> 585

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 585

atttcttggg agcactggac attcctgtaa gtccttgagt ccctcttgaa ctgtcttgtg 60

tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 120

<210> 586

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 586

tgtggtctga gggaacgagt tgttttaagg gcctatattt tctattcctg ggggtctgag 60

cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 120

<210> 587

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 587

cctttggttt tgttgggggc atggggccgc tgtgctgttc cctcgccgca ctgtctcggg 60

tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 120

<210> 588

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 588

tccctcatgc actgtctgag gctcttggcc tgtgtgggcc tttcctccct cacactaggt 60

cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 120

<210> 589

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 589

cagaggtaag aggcaagggc gaaatgggtt cctgtttcat ccccatctcc cgcaggtgcc 60

aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 120

<210> 590

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 590

aggcccaccc ccaggtgttc ccgcgcctgg gggcccaccc ctgtccaccg gacctatagt 60

ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 120

<210> 591

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 591

ggacctgctc cagtacagcc agaaggacct ggatgcagtg gtaaggacgc aggtagtagc 60

tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 120

<210> 592

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 592

tattccacag aacctgcagg ccgctctggg gcttggccaa cagtgggcag gggtccaaca 60

gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 120

<210> 593

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 593

gcctgacccg ccatccctgc catccctgcc cctgctggtt ggggtggaat tgcagagagc 60

tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 120

<210> 594

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 594

tgccggcccc tggcctgtca ctactgccca ggtcgctgag ggtccaggct tccacccagt 60

gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 120

<210> 595

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 595

gtccccgcag tcagctgccc accagcagcc tccccgggac tctccggcct gggcatggcg 60

ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 120

<210> 596

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 596

ttggcctctg gtggccttgc agcagtgagt gtggttcttg ctgccgtgag cccgagtgtc 60

gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 120

<210> 597

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 597

gcccctttcc tcccgccctc ccacgagtcc agacgcatct cctggcagct taggaagctc 60

catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 120

<210> 598

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 598

catcttcacc caccccacat ctgagggtcc cgtgccttgt ctccctccat cctcactgta 60

catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 120

<210> 599

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 599

catgccaggc ttcagcctgt tctaactccg ggcctaaatt gtccctaaga gagctgtagg 60

cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 120

<210> 600

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 600

cacacaaagg ggctgggtgt ctgcaggcac cactgtgagc taccgtcgcc agatggtccc 60

cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 120

<210> 601

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 601

cagggaggtg cagacagggt ggttccggag gccggctggc agggctgggt tcaagcccca 60

acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 120

<210> 602

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 602

acatccacac cagtgttgtt cacagtggcc ccaggtgtca tctccgcaga ggcctgtgat 60

gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 120

<210> 603

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 603

gtggccctga ccccgtaccc caacaacctc cttccctgtg tcagagcccg tgtgagtaac 60

ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 120

<210> 604

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 604

ccaatagcag gcctggggac cacagtgtgg ctggccccct cctctgcagg cctgggctcc 60

gggggcaggt ggcccctgca tgtaacttat gttagaaatg cacaatgctt gttctctggt 120

<210> 605

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 605

ctgccactgg gctgcggggg cagctgcagg gacacacacc tgttgggtgc ctgctggttc 60

cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 120

<210> 606

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 606

cacccaggag cacagccagc agcctcatgc ctcctgcccc ctggcagtcg ggtgcacgtg 60

gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 120

<210> 607

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 607

gagcaggtgg ccttggcctc gacagggttc ccagggtccc actggaagcc ccacatggaa 60

ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 120

<210> 608

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 608

ctgtgcctct gggacattgc tccagggacc tcgggtgcgg gctggcccca tcctgtgggg 60

aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 120

<210> 609

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 609

aggtcctggg agggtcagtc tggcccgcct gcctgctgac ttgggtgtgg cctgagcagg 60

taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 120

<210> 610

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 610

taaaggcgac acaggaggag aaccgggagc tgaggagcag gtgtgaggag ctccacggga 60

agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 120

<210> 611

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 611

agaacctgga actggggtaa ggaggccccg tctcctgtcc tccccgtccc catccccctc 60

gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 120

<210> 612

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 612

gccatccttg tccccatccc cctcgccatc cttgtcccca tccccctcgc catccctgtc 60

cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 120

<210> 613

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 613

cccgtcccca tccccacctg gcctgggacc gctgggcacc cgaggcaatg gctgtgtgtc 60

tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 120

<210> 614

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 614

tgttctcctc ccacaggaag atcatggaca ggttcgaaga ggttgtgtac caggccatgg 60

gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 120

<210> 615

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 615

gtgagtgccc gggccaccga ggccacgtgc ctccacgagg ggctgcctat gccccaccct 60

ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 120

<210> 616

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 616

ggccctgccc tgcaccctgc ctaggacccc accctcctga ggcccctttt cctaacacac 60

gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 120

<210> 617

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 617

gagtcccttc aacatgggcc cggccgtgga agcactgagg cgagttgcgg ggaagctggt 60

ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 120

<210> 618

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 618

ggtagtgagg ctggtggctc ccacggagcc agggcttggc aggccccaca gttgtgcaga 60

tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 120

<210> 619

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 619

tgctgagcta gcagtggggc tgtggtggga gcagagtctg tcccgccgtg gcctacccca 60

ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 120

<210> 620

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 620

ctccaccctg ccctcgccgt gcggctatgt ctagcagcag cctctgtagg tgtctggcag 60

gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 120

<210> 621

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 621

gacgggaaca ccaggcctca tctggcctcc caaaagcttg ggctcctgga ggtgctactg 60

tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 120

<210> 622

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 622

tggggatctg tcttaggctg agtgtcagcc ggcggcaggt gaccgcctgg cccttgcagt 60

ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 120

<210> 623

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 623

ccctccagga ccaccacgaa gcacgacgca catcctggag ctcgccatac cggctcccag 60

gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 120

<210> 624

<211> 120

<212> DNA

<213> Artificial

<220>

<221> misc_binding

<223>probe

<400> 624

gcctcaccct aggctctagg gtgttgggcg ccgcctcttc agggacctct ggcccaggct 60

ccagttccct gcggatctga tgtggaggaa acagctgttt cctgggcttt gcatccggcc 120

Claims (9)

1. the probe combinations for detecting human gene FGFR2/3 Gene Fusion, the probe combinations include following probes:

For the multicore glycosides of initiation site 1729490 on No. 4 chromosomes where people's TACC3 gene to termination site 1729609 Acid sequence or its complementary series, probe sequence are SEQ ID No:501,

For the multicore glycosides of initiation site 1729550 on No. 4 chromosomes where people's TACC3 gene to termination site 1729669 Acid sequence or its complementary series, probe sequence are SEQ ID No:502,

For the multicore glycosides of initiation site 1729610 on No. 4 chromosomes where people's TACC3 gene to termination site 1729729 Acid sequence or its complementary series, probe sequence are SEQ ID No:503,

For the multicore glycosides of initiation site 1729670 on No. 4 chromosomes where people's TACC3 gene to termination site 1729789 Acid sequence or its complementary series, probe sequence are SEQ ID No:504,

For the multicore glycosides of initiation site 1729730 on No. 4 chromosomes where people's TACC3 gene to termination site 1729849 Acid sequence or its complementary series, probe sequence are SEQ ID No:505,

For the multicore glycosides of initiation site 1729790 on No. 4 chromosomes where people's TACC3 gene to termination site 1729909 Acid sequence or its complementary series, probe sequence are SEQ ID No:506,

For the multicore glycosides of initiation site 1808662 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808781 Acid sequence or its complementary series, probe sequence are SEQ ID No:72,

For the multicore glycosides of initiation site 1808782 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808901 Acid sequence or its complementary series, probe sequence are SEQ ID No:79,

For the multicore glycosides of initiation site 1808722 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808841 Acid sequence or its complementary series, probe sequence are SEQ ID No:96,

For the multicore glycosides of initiation site 1808692 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808811 Acid sequence or its complementary series, probe sequence are SEQ ID No:269,

For the multicore glycosides of initiation site 1808752 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808871 Acid sequence or its complementary series, probe sequence are SEQ ID No:292,

For the multicore glycosides of initiation site 1808812 on No. 4 chromosomes where people's FGFR3 gene to termination site 1808931 Acid sequence or its complementary series, probe sequence are SEQ ID No:275,

For the more of initiation site 123241115 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241234 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:111,

For the more of initiation site 123241175 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241294 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:158,

For the more of initiation site 123241055 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241174 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:165,

For the more of initiation site 123240995 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241114 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:187,

For the more of initiation site 123241295 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241414 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:193,

For the more of initiation site 123241235 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241354 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:212,

For the more of initiation site 123240965 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241084 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:295,

For the more of initiation site 123241145 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241264 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:306,

For the more of initiation site 123241205 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241324 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:352,

For the more of initiation site 123241085 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241204 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:358,

For the more of initiation site 123241025 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241144 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:380,

For the more of initiation site 123241265 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241384 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:402,

For the more of initiation site 123240935 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241054 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:100,

For the more of initiation site 123242135 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242254 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:106,

For the more of initiation site 123243095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243214 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:109,

For the more of initiation site 123240755 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240874 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:113,

For the more of initiation site 123240815 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240934 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:114,

For the more of initiation site 123240275 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240394 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:115,

For the more of initiation site 123242195 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242314 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:121,

For the more of initiation site 123240515 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240634 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:123,

For the more of initiation site 123241655 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241774 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:125,

For the more of initiation site 123240335 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240454 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:128,

For the more of initiation site 123240635 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240754 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:129,

For the more of initiation site 123239735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239854 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:131,

For the more of initiation site 123240695 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240814 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:133,

For the more of initiation site 123241595 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241714 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:137,

For the more of initiation site 123239495 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239614 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:138,

For the more of initiation site 123239915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240034 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:139,

For the more of initiation site 123242735 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242854 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:143,

For the more of initiation site 123242975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243094 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:148,

For the more of initiation site 123240455 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240574 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:149,

For the more of initiation site 123240095 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240214 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:150,

For the more of initiation site 123241895 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242014 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:151,

For the more of initiation site 123240215 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240334 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:153,

For the more of initiation site 123242675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242794 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:156,

For the more of initiation site 123239975 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240094 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:157,

For the more of initiation site 123240575 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240694 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:160,

For the more of initiation site 123243035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243154 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:163,

For the more of initiation site 123239855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239974 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:164,

For the more of initiation site 123240155 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240274 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:167,

For the more of initiation site 123239795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239914 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:170,

For the more of initiation site 123241775 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241894 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:171,

For the more of initiation site 123242915 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243034 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:172,

For the more of initiation site 123242615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242734 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:175,

For the more of initiation site 123241955 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242074 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:182,

For the more of initiation site 123240875 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240994 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:183,

For the more of initiation site 123239555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239674 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:186,

For the more of initiation site 123242255 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242374 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:188,

For the more of initiation site 123240395 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240514 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:195,

For the more of initiation site 123239615 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239734 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:197,

For the more of initiation site 123240035 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240154 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:199,

For the more of initiation site 123242075 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242194 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:201,

For the more of initiation site 123241835 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241954 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:202,

For the more of initiation site 123241715 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241834 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:207,

For the more of initiation site 123242015 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242134 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:208,

For the more of initiation site 123242855 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242974 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:209,

For the more of initiation site 123242555 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242674 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:215,

For the more of initiation site 123239675 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239794 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:216,

For the more of initiation site 123242795 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242914 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:217,

For the more of initiation site 123242165 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242284 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:301,

For the more of initiation site 123243125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243244 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:304,

For the more of initiation site 123240785 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240904 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:308,

For the more of initiation site 123240845 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240964 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:309,

For the more of initiation site 123240305 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240424 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:310,

For the more of initiation site 123242225 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242344 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:316,

For the more of initiation site 123241685 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241804 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:319,

For the more of initiation site 123240365 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240484 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:322,

For the more of initiation site 123240665 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240784 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:323,

For the more of initiation site 123239765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239884 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:325,

For the more of initiation site 123240725 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240844 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:327,

For the more of initiation site 123241625 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241744 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:331,

For the more of initiation site 123239525 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239644 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:332,

For the more of initiation site 123239945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240064 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:333,

For the more of initiation site 123242765 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242884 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:337,

For the more of initiation site 123243005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243124 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:342,

For the more of initiation site 123240485 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240604 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:343,

For the more of initiation site 123240125 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240244 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:344,

For the more of initiation site 123241925 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242044 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:345,

For the more of initiation site 123240245 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240364 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:347,

For the more of initiation site 123242705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242824 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:350,

For the more of initiation site 123240005 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240124 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:351,

For the more of initiation site 123243065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243184 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:356,

For the more of initiation site 123239885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240004 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:357,

For the more of initiation site 123240185 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240304 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:360,

For the more of initiation site 123239825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239944 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:363,

For the more of initiation site 123241805 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241924 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:364,

For the more of initiation site 123242945 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243064 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:365,

For the more of initiation site 123242645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242764 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:368,

For the more of initiation site 123241985 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242104 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:375,

For the more of initiation site 123240905 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241024 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:376,

For the more of initiation site 123239585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239704 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:379,

For the more of initiation site 123240425 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240544 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:386,

For the more of initiation site 123239645 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239764 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:387,

For the more of initiation site 123240065 on No. 10 chromosomes where people's FGFR2 gene to termination site 123240184 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:389,

For the more of initiation site 123242105 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242224 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:391,

For the more of initiation site 123241865 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241984 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:392,

For the more of initiation site 123241745 on No. 10 chromosomes where people's FGFR2 gene to termination site 123241864 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:397,

For the more of initiation site 123242045 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242164 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:398,

For the more of initiation site 123242885 on No. 10 chromosomes where people's FGFR2 gene to termination site 123243004 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:399,

For the more of initiation site 123242585 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242704 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:405,

For the more of initiation site 123239705 on No. 10 chromosomes where people's FGFR2 gene to termination site 123239824 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:406,

For the more of initiation site 123242825 on No. 10 chromosomes where people's FGFR2 gene to termination site 123242944 Nucleotide sequence or its complementary series, probe sequence are SEQ ID No:407,

For the multicore glycosides of initiation site 1809043 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809162 Acid sequence or its complementary series, probe sequence are SEQ ID No:74,

For the multicore glycosides of initiation site 1809163 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809282 Acid sequence or its complementary series, probe sequence are SEQ ID No:78,

For the multicore glycosides of initiation site 1808983 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809102 Acid sequence or its complementary series, probe sequence are SEQ ID No:82,

For the multicore glycosides of initiation site 1809283 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809402 Acid sequence or its complementary series, probe sequence are SEQ ID No:86,

For the multicore glycosides of initiation site 1809223 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809342 Acid sequence or its complementary series, probe sequence are SEQ ID No:91,

For the multicore glycosides of initiation site 1809103 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809222 Acid sequence or its complementary series, probe sequence are SEQ ID No:92,

For the multicore glycosides of initiation site 1809343 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809462 Acid sequence or its complementary series, probe sequence are SEQ ID No:97,

For the multicore glycosides of initiation site 1809193 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809312 Acid sequence or its complementary series, probe sequence are SEQ ID No:274,

For the multicore glycosides of initiation site 1809013 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809132 Acid sequence or its complementary series, probe sequence are SEQ ID No:278,

For the multicore glycosides of initiation site 1809313 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809432 Acid sequence or its complementary series, probe sequence are SEQ ID No:282,

For the multicore glycosides of initiation site 1809253 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809372 Acid sequence or its complementary series, probe sequence are SEQ ID No:287,

For the multicore glycosides of initiation site 1809133 on No. 4 chromosomes where people's FGFR3 gene to termination site 1809252 Acid sequence or its complementary series, probe sequence are SEQ ID No:288.

2. probe combinations described in claim 1, wherein human gene FGFR2/3 Gene Fusion include FGFR2-COL14A1, FGFR3-TACC3 and FGFR3-BAIAP2L1.

3. probe combinations of any of claims 1 or 2 are in preparation for detecting human gene FGFR2/3 gene in subject's sample Purposes in the kit of fusion.

4. purposes as claimed in claim 3, wherein the sample includes tissue biopsy sample, FFPE sample and cfDNA sample, The subject is normal healthy subjects, suspects or be diagnosed as the subject with disease.

5. purposes as claimed in claim 3, wherein the sample includes DNA sample and liquid biopsy blood plasma.

6. purposes as claimed in claim 4, wherein the disease is cancer.

7. the kit for detecting human gene FGFR2/3 Gene Fusion comprising capture reagent, the capture reagent include Probe combinations of any of claims 1 or 2.

8. kit as claimed in claim 7 further includes one of following or a variety of: 1) build library reagent, 2) except probe with Outer capture reagent, 3) solid phase carrier and/or 4) high-flux sequence reagent.

9. kit according to any one of claims 8, wherein the library reagent of building includes archaeal dna polymerase, ligase, buffer and/or Quality-control product；The capture reagent in addition to probe includes sealer, captures buffer and/or cleaning solution；The solid phase carrier Including magnetic bead.