CN110184163A - A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function - Google Patents
A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function Download PDFInfo
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Abstract
The invention discloses a kind of preparation methods with the kudzuvine root wine for reducing hepatic injury function, include the following steps: the preparation of (1) kudzu root extract, comprising: A. extracts concentration, B. sour water solution, C. purifying resin；(2) kudzu root extract high-speed cutting is dissolved；50% is up to using puerarin content in the kudzu root extract of the method for the present invention preparation, using high-content kudzu root extract prepared by the present invention, cooperate high-speed cutting dissolving method of the invention, meltage of the Puerarin in wine body can be improved, to obtain the kudzuvine root wine that puerarin content reaches 40-80mg/L；Kudzuvine root wine of the present invention selects the folk prescription medicinal material pueraria lobata of integration of drinking and medicinal herbs medicinal material and health food catalogue, and kudzuvine root wine effective content obtained is high, lightly seasoned, color is shallow, while retaining white wine mouthfeel, have the function of that auxiliary reduces hepatic injury, the personage for being particularly suitable for often drinking drinks.
Description
Technical field
It is especially a kind of with the kudzuvine root wine for reducing hepatic injury function the present invention relates to functional health white wine technical field
Preparation method.
Background technique
Responsible drinking can promote human intestines and stomach to secrete, and help digest absorption, enhance blood circulation, promote tissue metabolism,
Increase cell viability.But excessively drink common white spirit for a long time, then injury, such as hepatic lesion can be generated to human body.Due to wine body
It is oxidized decomposition in the tangible liver of the first step after into human body, the damage to stand in the breach is exactly this internal organs of liver, it is desirable to be avoided
This damage, the most fundamental method are exactly abstinence from alcohol, but for the people that drinks of hobby or due to various work requirements, need frequency
It is numerous participate in the people that entertains of commercial affairs for, abstinence from alcohol be it is impossible, therefore, a kind of wine that can reduce hepar damnification is researched and developed, to full
The demand of drinking of this part population of foot is very necessary.
The principle for the impariment of the liver of drinking is: ethyl alcohol generates free radicals in liver metabolism approach and active oxygen, and initiated oxidation stress
Reaction, to induce liver organization damage.The factor relevant to oxidative stress has: malonaldehyde (MDA), superoxide dismutase
(SOD), glutathione peroxidase (GSH-PX).The content of MDA embodies level of lipid, the work of SOD and GSH-PX
Property embody Scavenger of ROS ability, they be relieving alcoholism and protecting liver research in common counter.There is antioxidant systems in liver
With the balance of enzymatic oxidation system, this balance will be broken after drinking for intake, SOD(superoxide dismutase) and GSH-PX(paddy Guang
Sweet peptide peroxidase) activity reduce.Therefore, the activity of antioxidant system in protective, remove interior free yl to prevention and treatment
Hepatic injury caused by oxidative stress is of great significance.
It is recorded in Compendium of Material Medica, pueraria lobata is a kind of plant of dual-purpose of drug and food, and gas is flat, sweet in flavor, has heat-clearing, fall fire, row
Poison and other effects, in China, pueraria lobata has the long history of more than one thousand years, and flower, root, powder are that the successive dynasties are clearing heat and detoxicating, promote blood circulation and sober up, protect
The key medicine of protecting liver, in ancient times even more a kind of official's medicine.Through modern medical research has found that, the ingredient that useful effect is played in pueraria lobata is main
It is Puerarin, also referred to as kudzu root extract is a kind of quasi-isoflavone class compound extracted from the Wild Pueraria without pollution.
The study found that Puerarin has significant protective effect to chronic liver injury animal model, mechanism may be with protection liver cell
It is related to remove the effects of free radical, anti peroxidation of lipid are with anti-fibrosis for film.Kudzu root extract is also known as " protect liver is precious ", is rich in liver
Dirty required multivitamin, glycogen, amino acid etc., the power of regeneration of liver cell had both can be improved in it, restored normal liver machine
Can, promote bile secretion, prevents fat from accumulating in liver；Can boost metabolism again, reinforce liver detoxification function, prevent due to
It is drunk caused by Ethanol intake excess, be still drank after a night, the damage to liver such as alcoholism, alcoholic liver；Liver and gallbladder cell can also be strengthened
Itself cotton dress function resists poisoning intrusion, has various oxyhepatitises caused by prevention liver disfunction.
It is easily excessive to drink high blood since diet time is long for often attending various commercial affairs and entertaining the personage of occasion
Sugar, high protein, high-fat, high cholesterol food, it is especially larger to the hepar damnification of human body, if it is possible to have researched and developed one kind both
It is able to satisfy commercial affairs to drink demand, and the health care wine of hepar damnification can be reduced, to needing often to drink the people of demand in face of commercial affairs
For scholar, it will be will be widely welcomed.
Summary of the invention
The purpose of the present invention is to current many people because many factors such as work, communication, living habit take often
The problem of drinking, damage inevitably brought to liver, and provide a kind of with the kudzuvine root wine for reducing hepatic injury function
Preparation method.
A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function of the invention, includes the following steps:
(1) preparation of kudzu root extract
A. concentration is extracted
Pueraria lobata raw material is put into extraction tank, food grade ethyl alcohol that 4-6 times of weight of pueraria lobata raw material is added, that mass fraction is 70%,
It impregnates 12-18 hours, material is heated to 60-85 DEG C, circumfluence distillation 1.5-2.5h, extract twice, combined extract, solid-liquid
The separation removal dregs of a decoction, collect clarification extracting solution；Extracting solution is transferred to dual-effect concentrator to be concentrated, is concentrated at 40-70 DEG C
Extracting solution raw medicinal herbs concentration is 2-3g/ml, obtains concentrate；
B. sour water solution
Concentrate is transferred in the enamel reaction still with agitating device, stirring is opened, sulfuric acid is slowly added in enamel reaction still
Or hydrochloric acid to hydrogen ion concentration reaches 0.5-1.5mol/l, after the completion of addition, material in reactor is warming up to 70-80 DEG C, water
Solution reaction 6-7 hours, filtering obtain hydrolyzate；
C. purifying resin
Prepare D-101 type or AB-8 type resin column, after activation processing, by hydrolyzate uniformly on on resin column, loading speed
For 1.7-2.3BV/h, sample solution is discarded, uses pure water to elute 4-6BV with the speed of 2-4BV/h, discards water lotion；It uses again
Mass fraction is the food grade ethyl alcohol of 43-47%, elutes 4-6BV with the speed of 2-4BV/h, collects ethanol eluate；Ethyl alcohol is washed
De- liquid is concentrated into raw medicinal herbs concentration 8-10g/ml, places, crystallisation by cooling, filters pressing separation, carries out 2-3 recrystallization, dry, powder
It is broken, cross 80 meshes, packaging is to get kudzu root extract, mass percentage >=50% of Puerarin in kudzu root extract obtained；
(2) kudzu root extract high-speed cutting is dissolved
It is transferred to the base liquor for accounting for emulsification tank volume 80% into emulsion tank, then the pueraria lobata of base liquor quality 0.5-1 ‰ is added into emulsion tank
Extract；The molten concentrated wiring liquid of kudzu root extract alcohol is formed, emulsion tank feeding port is closed, emulsion tank agitating device is opened and stirs 40 points
Clock, setting mixing speed are 1500-2000r/min；The kudzu root extract of base liquor quality 0.5-1 ‰ is added into emulsion tank again,
Stirring operation 40 minutes, then the kudzu root extract of base liquor quality 0.5-1 ‰ is added into emulsion tank, it is straight to repeat stirring operation ...
Into base liquor, the concentration of kudzu root extract reaches 5 ‰, and after stirring operation, the molten emulsion of the alcohol of kudzu root extract is stood
24-48h, then the polyethylene film filter that via hole diameter is 0.5 μm filter, and active carbon purifying filtering dispenses to get gained pueraria lobata
The content of Puerarin is 40-80mg/L in health liquor.
Heretofore described base liquor is delicate fragrance type, Luzhou-flavor or Maotai-flavor liquor.Can according to the mouthfeel demand of consumer,
The specific high quality liquor for selecting the different flavors such as delicate fragrance type, Luzhou-flavor, Maotai-flavor extracts object alcohol extracting, to obtain different perfume (or spice)
Type, different flavor, different quality class alcoholic drink.
The method of the present invention, mainly by the preparation method of special kudzu root extract, in addition the special molten technique of pueraria lobata alcohol,
Puerarin content in kudzuvine root wine is set to reach the amount of 40-80mg/L, to provide a kind of system of higher kudzuvine root wine of puerarin content
Preparation Method.
The kudzuvine root wine of the method for the present invention preparation, wine and women-sensual pursuits is bright orange, and faint scent is pure, and wine body is soft, sweet tasty and refreshing, all perfume harmonies,
Aftertaste is refreshing net, and faint scent, pueraria lobata perfume (or spice) have both at the same time.And by Disease Prevention Control Center, Hubei Prov " hair spread kudzuvine root wine with it is general
Comparative studies of the logical hair paving wine to subacute alcoholic liver injury " discovery, kudzu root extract is directly incorporated into wine by kudzuvine root wine to be drawn
With, under conditions of not influencing the mouthfeel of wine, it can have the function that reduce the oxidative damage that common white spirit generates matrix, from
And play the role of reducing hepatic injury after body is drunk.
Compared with prior art, the present invention advantage is as follows:
1. purifying Puerarin functional component in pueraria lobata raw material by single medicine extractive technique, and made by the method that high-speed cutting is dissolved
Puerarin alcohol as much as possible is molten into traditional liquor, obtains a kind of functional health white wine, while retaining white wine mouthfeel,
The healthcare function of hepatic injury is reduced with auxiliary；
It is damaged 2. largely drinking common white spirit for a long time and will lead to human liver, and drinks kudzuvine root wine of the invention, Ke Yiyou
Effect reduces hepar damnification, there is certain protective effect to human body health.
Kudzuvine root wine of the present invention selects the folk prescription medicinal material pueraria lobata of integration of drinking and medicinal herbs medicinal material and health food catalogue, kudzuvine root wine obtained
Effective content is high, and lightly seasoned, color is shallow, while retaining white wine mouthfeel, has the function of that auxiliary reduces hepatic injury, is particularly suitable for warp
The personage often to drink drinks.
Specific embodiment
Embodiment 1
A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function, includes the following steps:
(1) preparation of kudzu root extract
A. concentration is extracted
Pueraria lobata raw material is put into extraction tank, food grade ethyl alcohol that 5 times of weight of pueraria lobata raw material are added, that mass fraction is 70%, leaching
Material is heated to 70-80 DEG C, circumfluence distillation 2h by bubble 15 hours, is extracted twice, combined extract, and removal medicine is separated by solid-liquid separation
Slag collects clarification extracting solution；Extracting solution is transferred to dual-effect concentrator to be concentrated, extracting solution crude drug is concentrated at 50-60 DEG C
Material concentration is 2.58g/ml, obtains concentrate；
B. sour water solution
Concentrate is transferred in the enamel reaction still with agitating device, stirring is opened, sulfuric acid is slowly added in enamel reaction still
Reach 1.0mol/L to hydrogen ion concentration, after the completion of addition, material in reactor is warming up to 75 DEG C, hydrolysis 6.5 hours,
Filtering, obtains hydrolyzate；
C. purifying resin
Prepare D-101 type resin column, after activation processing, hydrolyzate is uniformly upper on resin column, and loading speed is 2.0BV/
H discards sample solution, uses pure water to elute 5BV with the speed of 3BV/h, discards water lotion；Use mass fraction for 45% again
Food grade ethyl alcohol elutes 5BV with the speed of 3BV/h, collects ethanol eluate；Ethanol eluate is concentrated into raw medicinal herbs concentration
9.1g/ml is placed, crystallisation by cooling, and filters pressing separation carries out 3 recrystallizations, be dry, pulverize, is crossed 80 meshes, packs to get pueraria lobata
Extract, the mass percentage of Puerarin is 56.8% in kudzu root extract obtained；
(2) kudzu root extract high-speed cutting is dissolved
It is transferred to the base liquor (800kg) for accounting for emulsion tank (1t) volume 80% into emulsion tank, then base liquor quality is added into emulsion tank
1 ‰ kudzu root extract；The molten concentrated wiring liquid of kudzu root extract alcohol is formed, emulsion tank feeding port is closed, opens emulsion tank agitating device
Stirring 40 minutes, setting mixing speed are 1500r/min；The kudzu root extract of base liquor quality 1 ‰ is added into emulsion tank again, stirs
Operation 40 minutes is mixed, then the kudzu root extract of base liquor quality 0.9 ‰ is added into emulsion tank, repeats stirring operation ... until base
The concentration of kudzu root extract reaches 5 ‰ in wine, and after stirring operation, the molten emulsion of the alcohol of kudzu root extract is stood 36h, then
The polyethylene film filter that via hole diameter is 0.5 μm filters, and active carbon purifying filtering dispenses to get Pueraria lobota in gained pueraria root health care liquor
The content of root element is 48.3mg/L.
Base liquor described in the present embodiment is that (base liquor used in the present invention is Jing Brand Co., Ltd.'s production pin to fen-flavor type white spirit
The 42 ° of hairs sold spread pure cereal wine, and the kudzuvine root wine produced is 42 ° of hairs paving kudzuvine root wine of commercial type.It can certainly adopt
With other base liquors, differently flavoured kudzuvine root wine is obtained.)
The effect of to verify kudzuvine root wine made from the present embodiment, applicant is in January, 2017 in Hubei Province's prevention and control of diseases
The heart, using made from the embodiment of the present invention 1 with preventing liver injury function kudzuvine root wine and common white spirit (be to adopt in the present embodiment
White wine test group is done with the base liquor for producing kudzuvine root wine in the present embodiment) it does about " being ground to the comparison of subacute alcoholic liver injury
Study carefully " subject study, specific research process and result be summarized as follows:
One, purpose
Evaluation repeats to give tested kudzuvine root wine after 42 days, compared with common white spirit (base liquor of production kudzuvine root wine), to the shadow of liver
It rings.
Two, method
After rat adaptable fed 3 days, be randomly divided into 5 groups by weight, respectively negative control group, base liquor control group and it is low, in,
High three dosage groups (16.7ml/kg BW, 33.3ml/kg BW, 50ml/kg Bw).Each group repeat stomach-filling give equal capacity by
Test agent or reference substance (negative control group gives pure water, base liquor control group gives base liquor) detect after 42 days: 1. abdominal aorta is adopted
Blood separates serum, detects GLU, AST, ALT, TC, TG.2. liver is weighed, liver/body ratio is calculated.3. taking serum or with even
Slurry examines MDA, SOD, LDH, ROS, xo-LDL.4. hepatic tissue is taken to prepare frozen section, pathology detection is carried out.
Three, result
Compared with negative control group, after to wine 42 days, rat body weight is begun to decline base liquor control group from second week.Biochemical indicator
Middle TC decline；Serum MDA increases in oxidative stress index, hepatic tissue ROS is increased, the decline of hepatic tissue LDH activity；Other biochemistry refer to
Mark, liver weight and liver/body ratio variation are little；Pathological examination result hepatic steatosis degree has increase, right with feminine gender
There is conspicuousness according to a group comparing difference.
To after wine 42 days, kudzuvine root wine each group is with base liquor control group compared with: to rat body weight, liver weighs and liver/body ratio,
Hepatic tissue LDH all has no significant effect；High dose group AST decline, each dosage group TC are increased, and have been not considered as clinical meaning；Oxidation is answered
Swash in index, each dosage group serum MDA, hepatic tissue ROS decline, and high dose group hepatic tissue xo-LDL is reduced, and difference has aobvious
Work property.Low, middle and high dose groups hepatic steatosis degree has reduction to pathological examination as the result is shown, compared with base liquor control group
Difference has conspicuousness.
Conclusion: repetition gave tested kudzuvine root wine after 42 days, it is possible to reduce and the oxidative damage that base liquor generates body is thin to liver
The mitigation of born of the same parents' steatosis has certain effect.
It can be seen that the kudzuvine root wine produced using the method for the present invention by above-mentioned comparative test, due to Puerarin in wine body
Content is high, really there is reduction base liquor to generate oxidative damage to body after drinking, to reduce base liquor to hepatic cell fattydegeneration
Mitigation effect.
Embodiment 2
A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function, includes the following steps:
(1) preparation of kudzu root extract
A. concentration is extracted
Pueraria lobata raw material is put into extraction tank, food grade ethyl alcohol that 4 times of weight of pueraria lobata raw material are added, that mass fraction is 70%, leaching
Material is heated to 75-85 DEG C, circumfluence distillation 1.5h by bubble 18 hours, is extracted twice, and combined extract is separated by solid-liquid separation removal
The dregs of a decoction collect clarification extracting solution；Extracting solution is transferred to dual-effect concentrator to be concentrated, it is raw that extracting solution is concentrated at 60-70 DEG C
Medicinal material concentration is 3.0g/ml, obtains concentrate；
B. sour water solution
Concentrate is transferred in the enamel reaction still with agitating device, stirring is opened, sulfuric acid is slowly added in enamel reaction still
Reach 1.5mol/L to hydrogen ion concentration, after the completion of addition, material in reactor is warming up to 80 DEG C, hydrolysis 7 hours, mistake
Filter, obtains hydrolyzate；
C. purifying resin
Prepare AB-8 type resin column, after activation processing, by hydrolyzate it is uniformly upper on resin column, loading speed is 2.3BV/h,
Sample solution is discarded, uses pure water to elute 6BV with the speed of 4BV/h, discards water lotion；Use again mass fraction for 43% food
With grade ethyl alcohol, 6BV is eluted with the speed of 4BV/h, collects ethanol eluate；Ethanol eluate is concentrated into raw medicinal herbs concentration
10.0g/ml is placed, crystallisation by cooling, and filters pressing separation carries out 3 recrystallizations, be dry, pulverize, is crossed 80 meshes, packs to get Pueraria lobota
Root extract, the mass percentage of Puerarin is 59.2% in kudzu root extract obtained；
(2) kudzu root extract high-speed cutting is dissolved
It is transferred to the base liquor (800kg) for accounting for emulsion tank (1t) volume 80% into emulsion tank, then base liquor quality is added into emulsion tank
0.8 ‰ kudzu root extract；The molten concentrated wiring liquid of kudzu root extract alcohol is formed, emulsion tank feeding port is closed, opens emulsion tank stirring dress
Stirring 40 minutes is set, setting mixing speed is 2000r/min；The pueraria lobata that base liquor quality 0.8 ‰ is added into emulsion tank again extracts
Object, stirring operation 40 minutes, then into emulsion tank be added base liquor quality 0.5 ‰ kudzu root extract, repeat stirring operation ...
Until the concentration of kudzu root extract reaches 5 ‰ in base liquor, after stirring operation, the molten emulsion of the alcohol of kudzu root extract is stood
48h, then the polyethylene film filter that via hole diameter is 0.5 μm filter, and active carbon purifying filtering dispenses to get gained pueraria root health care
The content of Puerarin is 73.6mg/L in wine.
Base liquor described in the present embodiment is Luzhou-flavor liquo.
Embodiment 3
A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function, includes the following steps:
(1) preparation of kudzu root extract
A. concentration is extracted
Pueraria lobata raw material is put into extraction tank, food grade ethyl alcohol that 6 times of weight of pueraria lobata raw material are added, that mass fraction is 70%, leaching
Material is heated to 60-70 DEG C, circumfluence distillation 2.5h by bubble 12 hours, is extracted twice, and combined extract is separated by solid-liquid separation removal
The dregs of a decoction collect clarification extracting solution；Extracting solution is transferred to dual-effect concentrator to be concentrated, it is raw that extracting solution is concentrated at 40-50 DEG C
Medicinal material concentration is 2.0g/ml, obtains concentrate；
B. sour water solution
Concentrate is transferred in the enamel reaction still with agitating device, stirring is opened, sulfuric acid is slowly added in enamel reaction still
Reach 0.5mol/L to hydrogen ion concentration, after the completion of addition, material in reactor is warming up to 70 DEG C, hydrolysis 6 hours, mistake
Filter, obtains hydrolyzate；
C. purifying resin
Prepare D-101 type resin column, after activation processing, hydrolyzate is uniformly upper on resin column, and loading speed is 1.7BV/
H discards sample solution, uses pure water to elute 4BV with the speed of 2BV/h, discards water lotion；Use mass fraction for 47% again
Food grade ethyl alcohol elutes 4BV with the speed of 2BV/h, collects ethanol eluate；Ethanol eluate is concentrated into raw medicinal herbs concentration
8.0g/ml is placed, crystallisation by cooling, and filters pressing separation carries out 3 recrystallizations, be dry, pulverize, is crossed 80 meshes, packs to get pueraria lobata
Extract, the mass percentage of Puerarin is 52.7% in kudzu root extract obtained；
(2) kudzu root extract high-speed cutting is dissolved
It is transferred to the base liquor (800kg) for accounting for emulsion tank (1t) volume 80% into emulsion tank, then base liquor quality is added into emulsion tank
0.9 ‰ kudzu root extract；The molten concentrated wiring liquid of kudzu root extract alcohol is formed, emulsion tank feeding port is closed, opens emulsion tank stirring dress
Stirring 40 minutes is set, setting mixing speed is 1500r/min；The pueraria lobata that base liquor quality 0.9 ‰ is added into emulsion tank again extracts
Object, stirring operation 40 minutes, then into emulsion tank be added base liquor quality 0.5 ‰ kudzu root extract, repeat stirring operation ...
Until the concentration of kudzu root extract reaches 5 ‰ in base liquor, after stirring operation, the molten emulsion of the alcohol of kudzu root extract is stood
For 24 hours, then via hole diameter filters for 0.5 μm of polyethylene film filter, and active carbon purifying filtering dispenses to get gained pueraria root health care
The content of Puerarin is 43.5mg/L in wine.
Base liquor described in the present embodiment is Maotai-flavor liquor.
Claims (2)
1. a kind of preparation method with the kudzuvine root wine for reducing hepatic injury function, it is characterised in that include the following steps:
(1) preparation of kudzu root extract
A. concentration is extracted
Pueraria lobata raw material is put into extraction tank, food grade ethyl alcohol that 4-6 times of weight of pueraria lobata raw material is added, that mass fraction is 70%,
It impregnates 12-18 hours, material is heated to 60-85 DEG C, circumfluence distillation 1.5-2.5h, extract twice, combined extract, solid-liquid
The separation removal dregs of a decoction, collect clarification extracting solution；Extracting solution is transferred to dual-effect concentrator to be concentrated, is concentrated at 40-70 DEG C
Extracting solution raw medicinal herbs concentration is 2-3g/ml, obtains concentrate；
B. sour water solution
Concentrate is transferred in the enamel reaction still with agitating device, stirring is opened, sulfuric acid is slowly added in enamel reaction still
Or hydrochloric acid to hydrogen ion concentration reaches 0.5-1.5mol/l, after the completion of addition, material in reactor is warming up to 70-80 DEG C, water
Solution reaction 6-7 hours, filtering obtain hydrolyzate；
C. purifying resin
Prepare D-101 type or AB-8 type resin column, after activation processing, by hydrolyzate uniformly on on resin column, loading speed
For 1.7-2.3BV/h, sample solution is discarded, uses pure water to elute 4-6BV with the speed of 2-4BV/h, discards water lotion；It uses again
Mass fraction is the food grade ethyl alcohol of 43-47%, elutes 4-6BV with the speed of 2-4BV/h, collects ethanol eluate；Ethyl alcohol is washed
De- liquid is concentrated into raw medicinal herbs concentration 8-10g/ml, places, crystallisation by cooling, filters pressing separation, carries out 2-3 recrystallization, dry, powder
It is broken, cross 80 meshes, packaging is to get kudzu root extract, mass percentage >=50% of Puerarin in kudzu root extract obtained；
(2) kudzu root extract high-speed cutting is dissolved
It is transferred to the base liquor for accounting for emulsification tank volume 80% into emulsion tank, then the pueraria lobata of base liquor quality 0.5-1 ‰ is added into emulsion tank
Extract；The molten concentrated wiring liquid of kudzu root extract alcohol is formed, emulsion tank feeding port is closed, emulsion tank agitating device is opened and stirs 40 points
Clock, setting mixing speed are 1500-2000r/min；The kudzu root extract of base liquor quality 0.5-1 ‰ is added into emulsion tank again,
Stirring operation 40 minutes, then the kudzu root extract of base liquor quality 0.5-1 ‰ is added into emulsion tank, it is straight to repeat stirring operation ...
Into base liquor, the concentration of kudzu root extract reaches 5 ‰, and after stirring operation, the molten emulsion of the alcohol of kudzu root extract is stood
24-48h, then the polyethylene film filter that via hole diameter is 0.5 μm filter, and active carbon purifying filtering dispenses to get gained pueraria lobata
The content of Puerarin is 40-80mg/L in health liquor.
2. a kind of preparation method with the kudzuvine root wine for reducing hepatic injury function according to claim 1, it is characterised in that:
The base liquor is delicate fragrance type, Luzhou-flavor or Maotai-flavor liquor.
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