CN110179452A - The production method and system of microelectrode, microelectrode battle array - Google Patents
The production method and system of microelectrode, microelectrode battle array Download PDFInfo
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- CN110179452A CN110179452A CN201910549897.6A CN201910549897A CN110179452A CN 110179452 A CN110179452 A CN 110179452A CN 201910549897 A CN201910549897 A CN 201910549897A CN 110179452 A CN110179452 A CN 110179452A
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Abstract
The present invention relates to microelectrode fields, the production method and system of a kind of microelectrode microelectrode battle array are disclosed, the production method of the microelectrode includes: to be socketed and seal in the side of tungsten bar to be fixed with glass capillary, and the first end of tungsten bar extends the first preset length except glass capillary, the second end of tungsten bar extends the second preset length except glass capillary；Control microelectrode executor clamps the lateral surface of glass capillary, to execute: being partially placed into for the first end for being socketed and being sealed with the tungsten bar of glass capillary is carried out electrolytic etching in strong base solution until the first end of tungsten bar is in needle point shape；First end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward and keeps the first preset time, wherein the first end of the tungsten bar exposes insulated paint dilution preset height；The insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains microelectrode.The present invention facilitates the production of microelectrode.
Description
Technical field
The present invention relates to the production method of microelectrode and systems.
Background technique
The various physiological functions of humans and animals are all directly or indirectly by the regulation of intracerebral neural network, and the nerve in network
Member can effectively change the information transfer characteristic between neuron by the variation of discharge frequency, to realize to various information
Processing.Therefore, the variation of discharge frequency feature under different physiological status of further investigation intracerebral neuron or neuron pool is
Disclose neural network mechanics and the important channel to physiological function regulating strategy.
The variation of record single unit discharge frequency needs impedance higher and durable microelectrode, can just collect in this way
Stronger electric signal, and observed and studied for a long time.The microelectrode being commonly used can be divided into two classes by material.One
Class is glass microelectrode, and this kind of microelectrode is although easy to make, but needs purchasing price expensive (100,000 yuans or so)
Drawing device, and glass microelectrode tip is easily damaged or by fragment of tissue blocking electrode tip in recording process.It is another kind of to be
Metal microelectrode, if wrapping glass capillary in metal microelectrode outer surface is also required to (such as tungsten filament electrode drawing of specific drawing device
Device processed) it could make, and the success rate for drawing the electrode of desired characteristic impedance is very low, is wrapped in the capillary glass of the needle point of metal microelectrode
Also it is easily damaged.In recent years, someone using some new technologies produce metal electrode array (such as Utah electrode array and
Michigan lineation electrode etc.), basic principle is to pass through thermophoresis, erosion using materials such as silicon, ceramics or glass as pedestal
It is micron-sized horizontal or longitudinal arrangement through-hole that the Mold Makings technologies such as quarter generate size on the base, and conductive metal needle point exists
Through hole is exposed to acquire electric signal, and through the circuit board input signal on pedestal to signal amplifier, but these metal electrodes
It is more complex with the manufacturing technology of electrode array, special instrument and equipment is needed, cost of manufacture is high, therefore it is (single to bring a high price in the market
Root metal electrode 100-200 member, metal electrode battle array 2000-3000 member), still, existing microelectrode service life is not long, micro- electricity
The first end (tip) of pole is easily-deformable or damages, and causes impedance to be too high to collect the signal of cell discharge, and occur
The electrode of obstacle can not also be repaired to reuse, therefore many laboratories are difficult to bear expense high in this way.
Summary of the invention
The object of the present invention is to provide a kind of microelectrode, the production method and system of microelectrode battle array, the microelectrodes, microelectrode
The production method and system of battle array, facilitate the production of microelectrode.
To achieve the goals above, the present invention provides a kind of production method of microelectrode, the production method packet of the microelectrode
It includes: being socketed and seal in the side of tungsten bar and be fixed with glass capillary, and the first end of the tungsten bar extends the capillary glass
First preset length except glass pipe, the second end of the tungsten bar extend the second preset length except the glass capillary；Control
Microelectrode executor processed clamps the lateral surface of the glass capillary, to execute: will be socketed and be sealed with the capillary glass
Being partially placed into for the first end of the tungsten bar of pipe carries out electrolytic etching in strong base solution until the first end of the tungsten bar is in needle
It is pointed；The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；Removal is put into absolutely
The insulated paint of the outside of the second end of the tungsten bar after edge paint dilution obtains microelectrode.
Preferably, the side in tungsten bar is socketed and seals that be fixed with glass capillary include: by capillary glass pipe sleeve
It is connected to the lateral surface of the tungsten bar, and passes through the both ends of dental cement or the fixed glass capillary of glue sealing.
Preferably, the strong base solution includes sodium hydroxide solution.
Preferably, the first end by the tungsten bar for being socketed and being sealed with the glass capillary be partially placed into it is strong
Electrolytic etching is carried out in aqueous slkali until it includes: that will be socketed and be sealed with the capillary glass that the first end of the tungsten bar, which is in needle point shape,
The first end of the tungsten bar of glass pipe invades the strong base solution predetermined depth repeatedly and carries out electrolytic etching, until the tungsten bar
First end is in needle point shape when taking out the strong base solution.
Preferably, by the first end in the tungsten bar of needle point shape first end upward and its second end is put into downward
After keeping the first preset time in insulated paint dilution, and the insulated paint in the outside for the second end for removing the tungsten bar obtains
Before the microelectrode, this method further include: take out the tungsten bar from the insulated paint dilution, and control hair dryer court and take
The tungsten bar the second preset time of blowing out；Repeat following step preset times: by the first end in described in needle point shape
The first end of tungsten bar is upward and its second end is put into insulated paint dilution downward and keeps the first preset time, and from the insulation
Paint dilution in take out the tungsten bar, and control hair dryer towards taking-up the tungsten bar dry the second preset time.
Preferably, the insulated paint that the removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains
Method to microelectrode includes: that the is kept in the second end intrusion dimethylbenzene by the tungsten bar being put into after insulated paint dilution
Three preset times take out the tungsten bar and obtain the microelectrode.
By above-mentioned embodiment, just accurately controlled using homemade electrolysis unit and existing microelectrode executor
The length of tungsten microelectrode first end, that is, tip corrosion, and test facilitate it is easily operated, once microelectrode first end is damaged
It is bad, directly removal japanning, then re-execute step of the invention and reparation can be realized, allow the microelectrode to repeat to make
With.
In addition, the present embodiment also provides a kind of manufacturing system of microelectrode, which includes: to be sleeved and fixed
Unit, is socketed and is sealed for the side in tungsten bar and be fixed with glass capillary, and the first end of the tungsten bar extend it is described
First preset length except glass capillary, the second end of the tungsten bar extend the second default length except the glass capillary
Degree；Control unit clamps the lateral surface of the glass capillary for controlling microelectrode executor, is also used to execute: will cover
Connect and be sealed with the first end of the tungsten bar of the glass capillary be partially placed into strong base solution carry out electrolytic etching it is straight
First end to the tungsten bar is in needle point shape；By the first end in needle point shape the tungsten bar first end upward and its second
End, which is put into downward in insulated paint dilution, keeps the first preset time, wherein it is dilute that the first end of the tungsten bar exposes the insulated paint
Release liquid preset height；The insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains micro- electricity
Pole.
Preferably, the unit that is sleeved and fixed is used to be socketed and seal in the side of tungsten bar to be fixed with glass capillary packet
It includes: for glass capillary to be socketed on to the lateral surface of the tungsten bar, and passing through dental cement or the fixed hair of glue sealing
The both ends of thin glass tube.
In addition, the present embodiment also provides a kind of production method of microelectrode battle array, the production method of the microelectrode battle array include:
The side of tungsten bar is socketed and seals and is fixed with capillary glass and is in control components A, and the first end of the tungsten bar extends the hair
First preset length except thin glass tube, the second end of the tungsten bar extend the second default length except the glass capillary
Degree；The parallel fixation in side of the glass capillary of multiple components As is obtained into part B, wherein each tungsten in the part B
The endpoint of the first end of stick is in same level；Control microelectrode executor clamps any hair in the part B
The lateral surface of thin glass tube, to execute: being partially placed into strong base solution for the first end of each tungsten bar of the part B is carried out electricity
Solution corrosion is until the first end of each tungsten bar of the part B is in needle point shape；It is in the tungsten bar of needle point shape by the first end
First end is upward and its second end is put into insulated paint dilution downward and the first preset time is kept to obtain component C, wherein the tungsten
The first end of stick exposes the insulated paint dilution preset height；Removal is put into the component C's after insulated paint dilution
The insulated paint of the outside of the second end of each tungsten bar obtains microelectrode.
In addition, the present embodiment also provides a kind of manufacturing system of microelectrode battle array, the manufacturing system of the microelectrode battle array includes: set
Fixed cell is connect, is socketed and seals for the side in tungsten bar and be fixed with capillary glass and be in control components A, and the of the tungsten bar
The first preset length except the glass capillary is extended in one end, and the second end of the tungsten bar extends the glass capillary
Except the second preset length；It is also used to the parallel fixation in side of the glass capillary of multiple components As obtaining part B,
Wherein, the endpoint of the first end of each tungsten bar is in same level in the part B；And control unit, it is micro- for controlling
Electrode executor clamps the lateral surface of any glass capillary in the part B, is also used to execute: by the part B
Each tungsten bar first end be partially placed into strong base solution carry out electrolytic etching until the part B each tungsten bar first end
In needle point shape；By the first end in the tungsten bar of needle point shape first end upward and its second end to be put into insulated paint downward dilute
It releases and the first preset time is kept to obtain component C in liquid, wherein the first end of the tungsten bar exposes the default height of the insulated paint dilution
Degree；And removal is put into the insulated paint of the outside of the second end of each tungsten bar of the component C after insulated paint dilution and obtains
Microelectrode.
The manufacturing system of the manufacturing system of above-mentioned microelectrode, the production method of microelectrode battle array and microelectrode battle array is relative to existing
Technology has technical effect identical with the production method of microelectrode, and details are not described herein.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
The drawings are intended to provide a further understanding of the invention, and constitutes part of specification, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is a kind of flow chart of the production method of the microelectrode of preferred embodiment of the invention；
Figure 1A is the structural schematic diagram after a kind of S110 of the invention；
Fig. 2 is implementation method A in a kind of Fig. 1 of preferred embodiment of the invention) structural schematic diagram；
Fig. 3 is implementation method B in a kind of Fig. 1 of preferred embodiment of the invention) device structural schematic diagram；And
Fig. 4 is a kind of module connection figure of the manufacturing system of the microelectrode of preferred embodiment of the invention.
Fig. 5 is the structural schematic diagram of the components A of the embodiment of the present invention three；
Fig. 6 is the shape and structure schematic diagram after Fig. 1 execution step A of the invention；And
Fig. 7 is the structural schematic diagram of the part B of the embodiment of the present invention three.
Description of symbols
26 needle sharp structure of 1a first end
1b second end 1c glass capillary
2 glass of 1d tungsten bar
3 clamping 4 strong base solutions of button
5 transformer, 6 plug
7 electric wire, 8 metal clip
9 microelectrode executor, 10 electrode jaw
11 adjusting knob, 21 transparent glass vessels
The 22 insulated paint dilution page, 23 magnifying glass
24 magnifying glass bracket, 25 components A
Specific embodiment
Below in conjunction with attached drawing, detailed description of the preferred embodiments.It should be understood that this place is retouched
The specific embodiment stated is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
In the present invention, in the absence of explanation to the contrary, the noun of locality used such as " upper and lower, left and right " typically refers to
It is as shown in Figure 1 up and down." inside and outside " refer in specific profile it is interior with it is outer." remote, close " refers to relative to some component
Far and closely.
Embodiment one
Fig. 1 is the flow chart of the production method of the microelectrode of an embodiment of the present invention.
As shown in Figure 1, the production method that the embodiment proposes a kind of microelectrode, specific as described below.
S110, is socketed and seals in the side of tungsten bar and be fixed with glass capillary, and the first end of the tungsten bar is extended
First preset length except the glass capillary, it is pre- that the second end of the tungsten bar extends except the glass capillary second
If length.Structure after S110 is as shown in Figure 1A.
Wherein, the tungsten bar can choose 0.5-1.0 millimeters of diameter of tungsten bar, and the tungsten bar is cut into suitable length
Degree, the suitable length can be 80 millimeters.It can be 2-3 milli in the glass capillary that the exterior lateral sides of the tungsten bar are socketed
Rice, the purpose of the design essentially consist in the clamping for facilitating microelectrode executor, facilitate subsequent operation.Wherein, described first is pre-
If length can be 8-10 millimeters of random length, the second preset length can be 15 millimeters.The first end is micro- for making
Eletrode tip, and carry out the operation such as corroding in the next steps, input of the second end for recording electrode signal connects.It is making
Facilitate after above-mentioned component out and executes following steps.
Specifically, in S110, glass capillary is socketed on to the lateral surface of the tungsten bar, and pass through dental cement or glue
The both ends of the fixed glass capillary of water-stop obtain structure as shown in Figure 1A.Wherein, dental cement or glue are all existing
What the stage can obtain, and the glue can be preferably 502 Instant cements.
S120, control microelectrode executor clamp the lateral surface of the glass capillary, are executed under clamping with facilitating
Following A), B) and C).
Wherein, Fig. 2 specifically discloses the structure of microelectrode executor and electrolysis unit, and control microelectrode executor clamps
The mode of the lateral surface of the glass capillary, can be as shown in Fig. 2, Fig. 2 discloses the structure of microelectrode executor, specifically
Include:
Electrode jaw 10, for clamping the lateral surface of the glass capillary；Adjusting knob, for adjusting the capillary
The height of glass tube.Specific structure is as shown in Fig. 2, it can drive the height of the glass capillary by the rotation of adjusting knob
Degree variation.As shown in Fig. 2, being provided with graduated scale on the panel of microelectrode executor, the scale of adjusting is used to indicate to indicate
Position where tungsten bar.
A) being partially placed into the first end for being socketed and being sealed with the tungsten bar of the glass capillary in strong base solution
Electrolytic etching is carried out until the first end of the tungsten bar is in needle point shape.Specific shape and structure such as Fig. 6 institute after step A)
Show, mainly sees needle sharp structure 26.
Wherein, as shown in Fig. 2, the electrolytic etching can be realized by electrolysis unit, in above-mentioned steps, specifically,
It is preset with power circuit, the power circuit includes plug 6, is connected to for connecting supply voltage 220v, and by electric wire 7
The input terminal of transformer 5 is clamped with the metal clip by 7 electric connection of power supply circuit output end of electric wire in the second end of the tungsten bar
8, it is also preset with the glass 2 of carrying strong base solution and is connected to the clamping that the strong base solution is set on the glass and buckle
3, the clamping is connected to by the metal clip 8 that electric wire 7 is electrically connected to the power circuit cathode and buckles 3.It will be socketed and be sealed with
The first end of the tungsten bar of the glass capillary invades the strong base solution 4 by rotation of adjustment knob 11 repeatedly and presets deeply
Degree carries out electrolytic etching (wherein, tungsten bar can generate visible bubble in strong base solution 4), passes through the microelectrode executor 9
Panel on be provided with the depth that graduated scale controls first end intrusion, and every subintrusion depth it is identical be all 2-3 millimeters,
The range of the graduated scale is 0-25 millimeters, and minimum scale is 0.5 millimeter, wherein needs to control every very short time described micro-
Electrode executor 9 takes out the tungsten bar, then observation (with artificial observation or can pass through infrared sensor induction) described tungsten bar
Whether first end is in needle point shape, when being in needle point shape, stops electrolytic etching, and cleaned.
B) as shown in figure 3, by the first end in needle point shape the tungsten bar first end upward and its second end downward
It is put into insulated paint dilution and keeps the first preset time, wherein it is pre- that the first end of the tungsten bar exposes the insulated paint dilution
If height.
Wherein, carry the insulated paint dilution is transparent glass vessels 21, and the first preset time is 1 minute, tungsten bar
First end expose 22 preset height of the insulated paint dilution page be 25 microns, wherein the mode declined be with 5 microns of step-length
Slowly decline, and be observed by magnifying glass 23 to guarantee that it protrudes insulated paint dilution preset height as 25 microns,
The lower section of magnifying glass 23 is provided with magnifying glass bracket 24, is used to support the magnifying glass 23.In actual operation, it can also use
Following concrete modes:
Specifically, by the first end in the tungsten bar of needle point shape first end upward and its second end is put into downward
After keeping the first preset time in insulated paint dilution, the tungsten bar is taken out from the insulated paint dilution, and control and blow
Tungsten bar blowing second preset time of the blower towards taking-up.
Then following step preset times are repeated:
By the first end in the tungsten bar of needle point shape first end upward and its second end to be put into insulated paint downward dilute
It releases and keeps the first preset time in liquid, and take out the tungsten bar from the insulated paint dilution, and control hair dryer towards taking-up
The tungsten bar dry the second preset time.
Wherein, the preset times are specifically to depend on the circumstances for 2-3 times, wherein control mode can be electronic mode can also
By be manually in a manner of, second preset time be 2-3 minute, wherein the wind blown out be warm wind.In addition insulation is stated in carrying
The transparent glass vessels for painting dilution can be in outer surface markers 0-2 millimeters of scales, and wherein minimum scale is 0.1 millimeter.In addition,
Practical insulation dilution described in the present embodiment is quick-dry type insulated paint, the specific can be that the insulating materials such as polyimides, separately
Outside, it is diluted according to the ratio of itself and dimethylbenzene 2:1, to configure insulated paint dilution, the tungsten bar can be prevented in this way
Surface is uneven.
C) insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains microelectrode.
Wherein, the second end for being put into the tungsten bar after insulated paint dilution can be invaded dimethylbenzene by the mode of removal
Middle holding third preset time, takes out the tungsten bar and obtains the microelectrode.
Wherein, the third preset time is 20 minutes.The main function of dimethylbenzene is to remove before on the second end
Insulated paint after finally drying again, could obtain the micro machine in order to make it connect electric signal probe.
Embodiment two
Fig. 4 is a kind of manufacturing system of microelectrode of the offer of embodiment two, which may include: set
Fixed cell is connect, is socketed and seals for the side in tungsten bar and be fixed with glass capillary, and the first end of the tungsten bar extends
The first preset length except the glass capillary, the second end of the tungsten bar are extended second except the glass capillary out
Preset length；Control unit clamps the lateral surface of the glass capillary for controlling microelectrode executor, is also used to hold
Row: being partially placed into strong base solution for first end for being socketed and being sealed with the tungsten bar of the glass capillary is electrolysed
Corrosion is until the first end of the tungsten bar is in needle point shape；By the first end in needle point shape the tungsten bar first end upward and
Its second end, which is put into downward in insulated paint dilution, keeps the first preset time, wherein the first end of the tungsten bar is exposed described exhausted
Edge paint dilution preset height；The insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains
To microelectrode.
Preferably, the unit that is sleeved and fixed is used to be socketed and seal in the side of tungsten bar to be fixed with glass capillary packet
It includes: for glass capillary to be socketed on to the lateral surface of the tungsten bar, and passing through dental cement or the fixed hair of glue sealing
The both ends of thin glass tube.
Preferably, described control unit is used to be socketed and be sealed with the first end of the tungsten bar of the glass capillary
Be partially placed into strong base solution carry out electrolytic etching until the tungsten bar first end in needle point shape may include: will be socketed simultaneously
The first end for being sealed with the tungsten bar of the glass capillary invades the strong base solution predetermined depth repeatedly and carries out electrolysis corruption
Erosion, until the first end of the tungsten bar is in needle point shape when taking out the strong base solution.
Preferably, described control unit be used for by the first end in needle point shape the tungsten bar first end upward and its
Second end is put into downward keep the first preset time in insulated paint dilution after, and removing the outer of the second end of the tungsten bar
Before the insulated paint in portion obtains the microelectrode, described control unit can be also used for taking out institute from the insulated paint dilution
State tungsten bar, and control hair dryer towards taking-up the tungsten bar dry the second preset time；And for repeating following step default time
Number: the first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into downward in insulated paint dilution
The first preset time is kept, and takes out the tungsten bar from the insulated paint dilution, and control hair dryer towards described in taking-up
Tungsten bar the second preset time of blowing.
Preferably, described control unit is used to be put into the outside of the second end of the tungsten bar after insulated paint dilution
The method that insulated paint obtains microelectrode includes:
Third preset time will be kept in the second end intrusion dimethylbenzene for being put into the tungsten bar after insulated paint dilution,
It takes out the tungsten bar and obtains the microelectrode.
There is the manufacturing system of above-mentioned microelectrode technology identical with the production method of microelectrode to imitate compared with the existing technology
Fruit, details are not described herein.
Embodiment three
Embodiment is third is that a kind of production method of microelectrode battle array, the production method of the microelectrode battle array include: in the side of tungsten bar
Surface cover connects and seals and is fixed with capillary glass and is in control components A, components A structure as shown in figure 5, and the first end of the tungsten bar prolong
The first preset length except the glass capillary is stretched out, the second end of the tungsten bar is extended except the glass capillary
Two preset lengths；The parallel fixation in side of the glass capillary of multiple components As is obtained into part B, wherein the component
The endpoint of the first end of each tungsten bar is in same level in B；Control microelectrode executor clamps any in the part B
The lateral surface of the glass capillary, to execute: by being partially placed into strong base solution for the first end of each tungsten bar of the part B
Electrolytic etching is carried out until the first end of each tungsten bar of the part B is in needle point shape, concrete shape is as shown in Figure 7；By described
One end in the tungsten bar of needle point shape first end upward and its second end is put into insulated paint dilution downward and keeps first pre-
If the time obtains component C, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；Removal is put into insulation
The insulated paint of the outside of the second end of each tungsten bar of the component C after paint dilution obtains microelectrode.
Wherein the control mode of embodiment three further includes the mode almost the same with embodiment one, can still execute reality
Each step of example one kind is applied, microelectrode only need to be revised as to micro machine battle array, details are not described herein.
The production method of the microelectrode battle array of above-mentioned microelectrode has the production method phase with microelectrode compared with the existing technology
Same technical effect, details are not described herein.
Example IV
Example IV provides a kind of manufacturing system of microelectrode battle array, and the manufacturing system of the microelectrode battle array includes: to be sleeved and fixed
Unit, is socketed and is sealed for the side in tungsten bar and be fixed with capillary glass and be in control components A, and the first end of the tungsten bar is prolonged
The first preset length except the glass capillary is stretched out, the second end of the tungsten bar is extended except the glass capillary
Two preset lengths；It is also used to the parallel fixation in side of the glass capillary of multiple components As obtaining part B, wherein institute
The endpoint for stating the first end of each tungsten bar in part B is in same level；And control unit, for controlling microelectrode manipulation
Device clamps the lateral surface of any glass capillary in the part B, is also used to execute: by each tungsten bar of the part B
Being partially placed into strong base solution for first end carry out electrolytic etching until the first end of each tungsten bar of the part B is in needle point
Shape；The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into downward in insulated paint dilution
The first preset time is kept to obtain component C, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；With
And removal is put into the insulated paint of the outside of the second end of each tungsten bar of the component C after insulated paint dilution and obtains micro- electricity
Pole.
Wherein the control mode of embodiment three further includes the almost the same structure of embodiment two, can still execute implementation
Microelectrode, only need to be revised as micro machine battle array, details are not described herein by a kind of each step of example.
The manufacturing system of the microelectrode battle array of above-mentioned microelectrode has the production method phase with microelectrode compared with the existing technology
Same technical effect, details are not described herein.
It is described the prefered embodiments of the present invention in detail above in conjunction with attached drawing, still, the present invention is not limited to above-mentioned realities
The detail in mode is applied, within the scope of the technical concept of the present invention, a variety of letters can be carried out to technical solution of the present invention
Monotropic type, these simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of production method of microelectrode, which is characterized in that the production method of the microelectrode includes:
It is socketed and seals in the side of tungsten bar and be fixed with glass capillary, and the first end of the tungsten bar extends the capillary glass
First preset length except glass pipe, the second end of the tungsten bar extend the second preset length except the glass capillary；
Control microelectrode executor clamps the lateral surface of the glass capillary, to execute:
Being partially placed into strong base solution for the first end for being socketed and being sealed with the tungsten bar of the glass capillary is subjected to electricity
Solution corrosion is until the first end of the tungsten bar is in needle point shape；
The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；
The insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains microelectrode.
2. the production method of microelectrode according to claim 1, which is characterized in that described in the socket of the side of tungsten bar and close
Sealing has the glass capillary to include: surely
Glass capillary is socketed on to the lateral surface of the tungsten bar, and passes through dental cement or the fixed capillary glass of glue sealing
The both ends of glass pipe.
3. the production method of microelectrode according to claim 1, which is characterized in that the strong base solution includes sodium hydroxide
Solution.
4. the production method of microelectrode according to claim 1, which is characterized in that described to be socketed and be sealed with the hair
Being partially placed into strong base solution for the first end of the tungsten bar of thin glass tube carries out electrolytic etching up to the first of the tungsten bar
End includes: in needle point shape
It is default deep that the first end for being socketed and being sealed with the tungsten bar of the glass capillary is invaded into the strong base solution repeatedly
Degree carries out electrolytic etching, until the first end of the tungsten bar is in needle point shape when taking out the strong base solution.
5. the production method of microelectrode according to claim 1, which is characterized in that be in needle point shape by the first end
The first end of the tungsten bar is upward and its second end is put into downward after keeping the first preset time in insulated paint dilution, and
Remove the outside of the second end of the tungsten bar insulated paint obtain the microelectrode before, this method further include:
The tungsten bar is taken out from the insulated paint dilution, and the tungsten bar blowing second for controlling hair dryer towards taking-up is default
Time；
Repeat following step preset times:
The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding, and take out the tungsten bar from the insulated paint dilution, and control hair dryer towards taking-up institute
State tungsten bar the second preset time of blowing.
6. the production method of microelectrode described in any one of -5 according to claim 1, which is characterized in that the removal is put into
The method that the insulated paint of the outside of the second end of the tungsten bar after insulated paint dilution obtains microelectrode includes:
Third preset time will be kept in the second end intrusion dimethylbenzene for being put into the tungsten bar after insulated paint dilution, taken out
The tungsten bar obtains the microelectrode.
7. a kind of manufacturing system of microelectrode, which is characterized in that the microelectrode manufacturing system includes:
It is sleeved and fixed unit, is socketed and seals for the side in tungsten bar and be fixed with glass capillary, and the first of the tungsten bar
The first preset length except the glass capillary is extended at end, the second end of the tungsten bar extend the glass capillary it
Outer second preset length；
Control unit clamps the lateral surface of the glass capillary for controlling microelectrode executor, is also used to execute:
Being partially placed into strong base solution for the first end for being socketed and being sealed with the tungsten bar of the glass capillary is subjected to electricity
Solution corrosion is until the first end of the tungsten bar is in needle point shape；
The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；
The insulated paint that removal is put into the outside of the second end of the tungsten bar after insulated paint dilution obtains microelectrode.
8. the manufacturing system of microelectrode according to claim 7, which is characterized in that the unit that is sleeved and fixed is in tungsten
The side of stick is socketed and seals and is fixed with glass capillary and includes:
For glass capillary to be socketed on to the lateral surface of the tungsten bar, and pass through dental cement or the fixed hair of glue sealing
The both ends of thin glass tube.
9. a kind of production method of microelectrode battle array, which is characterized in that the production method of the microelectrode battle array includes: in the side of tungsten bar
Be socketed and seal and be fixed with capillary glass and be in control components A, and the first end of the tungsten bar extend the glass capillary it
Outer first preset length, the second end of the tungsten bar extend the second preset length except the glass capillary；
The parallel fixation in side of the glass capillary of multiple components As is obtained into part B, wherein each tungsten in the part B
The endpoint of the first end of stick is in same level；
Control microelectrode executor clamps the lateral surface of any glass capillary in the part B, to execute:
Being partially placed into strong base solution for the first end of each tungsten bar of the part B is subjected to electrolytic etching until the part B
Each tungsten bar first end be in needle point shape；
The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding obtains component C, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；
The insulated paint that removal is put into the outside of the second end of each tungsten bar of the component C after insulated paint dilution obtains micro- electricity
Pole.
10. a kind of manufacturing system of microelectrode battle array, which is characterized in that the manufacturing system of the microelectrode battle array includes:
It is sleeved and fixed unit, is socketed and seals for the side in tungsten bar and be fixed with capillary glass and be in control components A, and the tungsten
The first end of stick extends the first preset length except the glass capillary, and the second end of the tungsten bar extends the capillary
Second preset length except glass tube；It is also used to obtain the parallel fixation in side of the glass capillary of multiple components As
Part B, wherein the endpoint of the first end of each tungsten bar is in same level in the part B；And
Control unit clamps the lateral surface of any glass capillary in the part B for controlling microelectrode executor,
It is also used to execute:
Being partially placed into strong base solution for the first end of each tungsten bar of the part B is subjected to electrolytic etching until the part B
Each tungsten bar first end be in needle point shape；
The first end is in the first end of the tungsten bar of needle point shape upward and its second end is put into insulated paint dilution downward
The first preset time of middle holding obtains component C, wherein the first end of the tungsten bar exposes the insulated paint dilution preset height；
And
The insulated paint that removal is put into the outside of the second end of each tungsten bar of the component C after insulated paint dilution obtains micro- electricity
Pole.
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| CN1891142A (en) * | 2005-07-07 | 2007-01-10 | 中国科学院生物物理研究所 | Microelectrode making method and apparatus |
| CN101194831A (en) * | 2006-12-08 | 2008-06-11 | 通用电气公司 | Self-adhering electrodes and methods of making the same |
| CN101685091A (en) * | 2008-09-24 | 2010-03-31 | 西北师范大学 | Method of manufacturing an ultra-microelectrode |
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