CN110157612A - A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and the method using its progress hydrogen manufacturing - Google Patents
A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and the method using its progress hydrogen manufacturing Download PDFInfo
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Abstract
The invention discloses a kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and the methods for carrying out hydrogen manufacturing using it, the combined reactor includes cylinder and cabinet, cylinder is by top plate, the cylindrical side wall of bottom plate sum forms, the first cylindrical partition and the second cylindrical partition are successively arranged in cylinder from outside to inside, to sequentially form photosynthetic bacteria cultivation region from outside to inside, keep the temperature lighting area, light fermentation and hydrogen production area, it is successively arranged first partition from left to right in the cabinet, second partition, to sequentially form grown cultures basal cell from left to right, clean water chamber, produce hydrogen-based matter room, grown cultures basal cell is connected to by hose with photosynthetic bacteria cultivation region, clean water chamber is connected to heat preservation lighting area by hose, is produced from hydrogen-based matter room to be connected to by hose with light fermentation and hydrogen production area.The present invention can be realized photosynthetic bacteria culture and light fermentation and hydrogen production shares a set of illumination and attemperator, realizes and maximally utilizes to luminous energy and thermal energy.
Description
Technical field
The present invention relates to the technical fields of biomass photosynthetic organism hydrogen generation, and in particular to a kind of photosynthetic bacteria culture-light hair
The method that ferment produces hydrogen combined reactor and carries out hydrogen manufacturing using it.
Background technique
Since 21st century, energy shortage, environmental pollution are blocked in face of the whole mankind just as two mountains, therefore,
It is imperative to develop and inquire into new clean energy resource.As a kind of important material base, the development and existence of the mankind is all
It is be unable to do without the energy, with the development of the society, in today that economic development is maked rapid progress, energy-related problem has attracted
Global sight, high efficiency, low cost the sustainable use of Development of Novel new cleaning fuel, it has also become today's society hair
One of emphasis of exhibition.In face of the important topic of this 21st century, every country is all included in the development of new energy national new
Strategical planning in, or even formulated relevant state basic policy.In new energy field, the cleaning paid close attention to by industry
The speed development to attract people's attention of free of contamination hydrogen utilization technology, many developed countries are by the Hydrogen Energy of " cleaning "
Source is considered as the energy in national future and greatly speeds up research speed.Hydrogen Energy itself is a kind of secondary energy sources, it can only be some
It is extracted from non-renewable energy under the action of technological means.As a kind of ideal alternative energy source, the advantage of Hydrogen Energy is many
It is more, such as: combustibility is very well, calorific value is very high, resource is relatively abundanter etc.；As a kind of ideal alternative energy source, biological hydrogen production
It is polluted less with it, reaction condition is mild, the low plurality of advantages of energy consumption, the extensive concern by countries in the world.
In biological hydrogen production, the advantage of photosynthetic bacteria hydrogen production generates hydrogen it is clear that bacterium passes through degradation organic waste,
Hydrogen will be produced to combine with except useless, not only realized recycling for resource, but also effectively can convert Hydrogen Energy for the luminous energy of solar energy,
It is in turn electric energy by fuel cells convert, entire conversion process is pollution-free, low energy consumption, easy to operate.And various photosynthetic bacterias
Exist extensively in the environment, is easy to get, hydrogen generation efficiency is higher, and light conversion ratio is also higher.So photosynthetic organism hydrogen generation not only has
Certain economic benefit is a kind of very with the hydrogen manufacturing mode of development prospect there are also certain environmental benefit.And present light
It closes biological hydrogen production and even rests on the laboratory research stage, research light ferment for hydrogen production reactor is photosynthetic organism hydrogen generation technology from reality
Test the important ring that room is moved towards the industrialization.
Summary of the invention
The purpose of the present invention is to provide a kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor and utilize its progress
The method of hydrogen manufacturing.The device can be realized photosynthetic bacteria culture and light fermentation and hydrogen production shares a set of illumination and attemperator, realize
Luminous energy and thermal energy are maximally utilized, and increase the light-receiving area of photosynthetic bacteria, reduces the heat waste of light fermentation and hydrogen production
It loses, reduces energy consumption, improve the utilization efficiency to the energy, reduce operating cost.Bacteria Culture area and light are sent out with solenoid valve
The setting that the area Jiao Chanqing is connected to, the convenient operation that photosynthetic bacteria inoculation is carried out to light fermentation and hydrogen production area, reduces test work
It measures.
Based on above-mentioned purpose, the present invention is adopted the following technical scheme that:
A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor, including the cylinder being set on the first support and set on second
Cabinet on support, cylindrical side wall of the cylinder by top plate and bottom plate and between bottom plate and top plate form, from outer in cylinder
It is successively arranged the first cylindrical partition and the second cylindrical partition to interior, is formed between cylindrical side wall and the first cylindrical partition
Photosynthetic bacteria cultivation region, forms heat preservation lighting area between the first cylindrical partition and the second cylindrical partition, second it is cylindrical every
Light fermentation and hydrogen production area is formed in disconnected, is successively arranged first partition, second partition, first partition and case from left to right in the cabinet
Space between body left side wall forms grown cultures basal cell, the space formation clean water chamber between first partition, second partition, and second
Space between partition and cabinet right side wall, which is formed, produces hydrogen-based matter room, and grown cultures basal cell passes through hose and photosynthetic bacteria cultivation region
Connection, clean water chamber is connected to by hose with heat preservation lighting area, produces hydrogen-based matter room and be connected to by hose with light fermentation and hydrogen production area, photosynthetic
Bacteria Culture area, heat preservation lighting area are equipped with temperature sensor in light fermentation and hydrogen production area, and three temperature sensors are respectively and temperature
Controller connection.
Further, it is equipped with rabbling mechanism in the second cylindrical partition, the rabbling mechanism includes motor, paddle and stirs
Axis is mixed, motor is set on top plate, and agitating shaft passes through top plate and top is connected with motor, and agitating shaft, which extends downwardly, protrudes into light fermentation production
The lower part in hydrogen area, paddle are fixed on agitating shaft, and the outer wall of the second cylindrical partition is equipped with light source and (is specially wound with
LED light strip), top plate corresponding to light fermentation and hydrogen production area, which is equipped with, produces hydrogen outlet conduit, produces hydrogen outlet conduit and is equipped with gas stream
Meter keeps the temperature bottom plate corresponding to lighting area and is equipped with electric heating wire, the lower part of photosynthetic bacteria cultivation region and light fermentation and hydrogen production area
Equipped with communicating pipe, communicating pipe is equipped with solenoid valve；Shut-off valve and peristaltic pump are equipped on every hose.
Further, grown cultures basal cell, clean water chamber, produce hydrogen-based matter room top be equipped with feed inlet, grown cultures basal cell,
Clean water chamber, the lower part for producing hydrogen-based matter room are respectively equipped with sample outlet pipe road, and sample outlet pipe road is equipped with sample output valve, cylindrical side wall, first
The lower part of cylinder partition and the second cylindrical partition is equipped with discharge pipe, and discharge pipe is equipped with outlet valve.
Further, cylinder, the first cylindrical partition, the second cylindrical partition, cabinet, first partition, second partition are equal
For transparent material, specially glass or organic glass, the photosynthetic bacteria cultivation region, heat preservation lighting area, light fermentation and hydrogen production area its
Volume is denoted as V respectively1 、V2、V3；And V2:V1>=1, V1:V3>=1:2, grown cultures basal cell, produce hydrogen-based matter room, body at clean water chamber
Product is respectively V4、V5、V6；The ratio between volume in correspondence area that each building volume communicates therewith is equal to or more than 6:5, i.e. V4 :V1>=6:5,
V5 :V2>=6:5, V6 :V3≥6:5。
The method for carrying out hydrogen manufacturing using above-mentioned photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor, including following step
It is rapid:
A: the photosynthetic bacterium growth culture prepared in proportion is separately added into grown cultures basal cell and clean water chamber by feed inlet
Base and clear water connect wriggling pumping source, are delivered to photosynthetic bacteria cultivation region and heat preservation lighting area respectively, connect light source and electric heating wire
Power supply cultivates 48 ~ 72 h；
B: production hydrogen-based qualitative response liquid is added to is produced from hydrogen-based matter room by feed inlet；Wriggling pumping source is connected, produces hydrogen to photo fermentation
Area, which is added, produces hydrogen-based qualitative response liquid；Power of electromagnetic valve is connected, it is photosynthetic thin from photosynthetic bacteria cultivation region to the conveying of light fermentation and hydrogen production area
Bacterium, photosynthetic bacteria conveying capacity are react total volume 20% ~ 30%；
C: hydrogen container is set in the exit for producing hydrogen outlet conduit, is recorded by gas flowmeter and produces air volume；
D: valve is closed, improves gas production rate using the mode of intermittent stirring.
Preferably, the HAU-M1 photosynthetic hydrogen production bacterium that the photosynthetic bacteria strain is provided from Agricultural University Of He'nan, with number
Amount than meter, by 9% hydrogenlike silicon ion, 27% Rhodospirillum rubrum, 11% Rhodobacter capsulatus, 28% Rhodopseudomonas palustris and
25% capsula Rhodopseudomonas composition.
Preferably, the concrete composition of photosynthetic bacterium growth culture medium: yeast extract 1.0 g/L of 0.5 g/L, NaCl,
MgSO4·7H2O 0.1 g/L, CH3COONa 2.0 g/L, K2HPO40.1 g/L, NaHCO31.0 g/L, NH4Cl 0.5 g/
L, surplus are distilled water.
Preferably, the composition of hydrogen-based qualitative response liquid is produced are as follows: by sodium glutamate, NaCl, K2HPO4, yeast extract, MgCl2、
NH4Cl, biomass straw powder, cellulase are added in buffer, make 3.56 g/L of concentration of sodium glutamate, NaCl concentration
For 2.0 g/L, K2HPO4Concentration is 0.50 g/L, and yeast extract concentration is 0.10 g/L, MgCl2Concentration is 0.20 g/L,
NH4Cl concentration is 0.40 g/L, and cellulase is added according to 100mg/g stalk, and biomass straw powder is effective according to 25g/L
Volume addition, is uniformly mixed, and effective volume is produces hydrogen-based qualitative response liquid product and added the sum of photosynthetic bacteria volume；Its
Middle buffer is the citric acid-sodium citrate solution of PH=4.8.
Further, the reaction temperature in the photosynthetic bacteria cultivation region and light fermentation and hydrogen production area is 30 ± 1 DEG C；It is described
The intensity of illumination of LED light strip is 2500 ~ 3500 luxs.
The present apparatus can be realized photosynthetic bacteria culture and light fermentation and hydrogen production shares a set of illumination and attemperator, realize pair
Luminous energy and thermal energy maximally utilize, and increase the light-receiving area of photosynthetic bacteria, reduce the heat loss of light fermentation and hydrogen production, drop
Low energy consumption improves the utilization efficiency to the energy；The setting for being connected to Bacteria Culture layer with light fermentation layer with solenoid valve, side
Just the operation that photosynthetic bacteria inoculation is carried out to light fermentation layer, reduces test work load；With peristaltic pump by culture medium case, produce hydrogen-based
The setting of matter case connection, simplifies existing process for making hydrogen process.In addition, present apparatus occupied area is small, operating method is simple, relatively hold
It easily realizes modernization, can be widely applied to the industries such as the energy, environmental protection, have a good application prospect.
Detailed description of the invention
Fig. 1 is photosynthetic bacteria culture of the present invention-light fermentation and hydrogen production combined reactor structural schematic diagram；
Fig. 2 is the structural schematic diagram of cylindrical tube in Fig. 1；
In figure: 1. first supports, 2. bottom plates, 3. electric heating wires, 4. communicating pipes, 5. solenoid valves, 6. temperature controllers, 7. temperature pass
Sensor, 8. photosynthetic bacteria cultivation regions, 9. heat preservation lighting areas, 10. smooth fermentation and hydrogen production areas, 11. agitating shafts, 12. motors, 13. produce
Hydrogen outlet conduit, 14. gas flowmeters, 15. paddles, 16.LED light bar, 17. second cylindrical partitions, 18. first is cylindrical
Partition, 19. cylindrical side walls, 20. discharge pipes, 21. outlet valves, 22. shut-off valves, 23. hoses, 24. peristaltic pumps, 25. chargings
Mouthful, 26. grown cultures basal cells, 27. clean water chambers, 28. production hydrogen-based matter rooms, 29. sample outlet pipe roads, 30. sample output valves, 31. second supports,
32. top plate.
Specific embodiment
It is further described below in conjunction with attached drawing, the present embodiment gives detailed implementation premised on the technical program
Mode and specific operating process.
A kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor as depicted in figs. 1 and 2, including is set to the first support
Cylindrical tube on 1 and the rectangular box on the second support 31, cylindrical tube is by top plate 32, bottom plate 2 and sets
Cylindrical side wall 19 between bottom plate 2 and top plate 32 forms, and is successively arranged the first cylinder in cylindrical tube body from outside to inside
Partition 18 and the second cylindrical partition 17, form photosynthetic bacteria cultivation region between cylindrical side wall 19 and the first cylindrical partition 18
8, heat preservation lighting area 9, shape in the second cylindrical partition 17 are formed between the first cylindrical partition 18 and the second cylindrical partition 17
At light fermentation and hydrogen production area 10, be successively arranged first partition, second partition in the rectangular box from left to right, first partition and
Space between cabinet left side wall forms grown cultures basal cell 26, and the space between first partition, second partition forms clean water chamber
27, space between second partition and cabinet right side wall, which is formed, produces hydrogen-based matter room 28, grown cultures basal cell 28 by hose 23 with
Photosynthetic bacteria cultivation region 8 is connected to, and clean water chamber 27 is connected to heat preservation lighting area 9 by hose 23, is produced hydrogen-based matter room 28 and is passed through hose
23 are connected to light fermentation and hydrogen production area 20, and photosynthetic bacteria cultivation region 8, heat preservation lighting area 9 are equipped with temperature in light fermentation and hydrogen production area 10
Sensor 7, three temperature sensors 7 are connected with temperature controller 6 respectively.Temperature controller 6 is connected to external controlling terminal
To which signal is reached control centre, this belongs to technological means well known in the art and is not belonging to inventive point of the invention, herein
It repeats no more.
Further, it is equipped with rabbling mechanism in the second cylindrical partition 17, the rabbling mechanism includes motor 12, paddle
15 and agitating shaft 11, motor 12 is set on top plate 32, and agitating shaft 11 passes through top plate 32 and top is connected with motor 12, agitating shaft 11
The lower part for protruding into light fermentation and hydrogen production area 10 is extended downwardly, paddle 15 is fixed on agitating shaft 11, the second cylindrical partition 17
Being fastened on outer wall has LED light strip 16, and top plate corresponding to light fermentation and hydrogen production area 10, which is equipped with, produces hydrogen outlet conduit 13, produces hydrogen
Outlet conduit 13 is equipped with gas flowmeter 14, keeps the temperature bottom plate corresponding to lighting area 9 and is equipped with electric heating wire 3, photosynthetic bacteria
The lower part in cultivation region 8 and light fermentation and hydrogen production area 10 is equipped with communicating pipe 4, communicating pipe 4 is equipped with solenoid valve 5；On every hose 23
Equipped with shut-off valve 22 and peristaltic pump 24.
Further, grown cultures basal cell 26, clean water chamber 27, the top for producing hydrogen-based matter room 28 are grown equipped with feed inlet 25
Culture Medium Laboratory 26, clean water chamber 27, the lower part for producing hydrogen-based matter room 28 are respectively equipped with sample outlet pipe road 29, and sample outlet pipe road 29 is equipped with sample
The lower part of valve 30, the cylindrical partition 18 of cylindrical side wall 19, first and the second cylindrical partition 17 is equipped with discharge pipe 20, out
Pipe material 20 is equipped with outlet valve 21.
Further, cylindrical tube, the first cylindrical the 18, second cylindrical partition of partition 17, rectangular box, first
Partition, second partition are transparent material, for example glass or organic glass, the photosynthetic bacteria cultivation region 8, heat preservation lighting area 9,
10 its volume of light fermentation and hydrogen production area is denoted as V respectively1 、V2、V3；And V2:V1>=1, V1:V3>=1:2, grown cultures basal cell 26, clear water
Room 27 produces hydrogen-based matter room 28, and volume is respectively V4、V5、V6；The ratio between the volume in the correspondence area that each building volume communicates therewith is equal to
Or it is greater than 6:5, i.e. V4 :V1>=6:5, V5 :V2>=6:5, V6 :V3≥6:5。
In the present embodiment, a length of 400 mm of first support 1, width are 400 mm, a height of 500 mm, the cylinder
A height of 1000 mm of cylinder, radius are 180 mm, and the first 18 radiuses of cylindrical partition are 150 mm, the second cylindrical partition 17 half
Diameter is 100 mm.The cylindrical tube, the first cylindrical partition 17 of cylindrical partition 18, second are made of organic glass.
In the present embodiment, the turn-to-turn of 16 uniform winding of illuminating LED lamp band is away from for 80 mm, electric heating wire 3 fixed half
Diameter is 165 mm, and power is 2000 W.
In the present embodiment, agitating shaft 11 is the metallic rod of 800 mm of length, is being welded at 12 lower end of motor, 350 mm
First paddle 15 is connect, second paddle 15 is being welded at 12 lower end of motor, 700 mm.
In the present embodiment, a length of 500 mm of second support 31, width are 500 m, a height of 500 mm；The growth
The length (length in the front-back direction, similarly hereinafter) of Culture Medium Laboratory 26 is 500 mm, and wide (length of left and right directions, similarly hereinafter) is 130 mm,
A height of 600 mm；A length of 500 mm of the clean water chamber 27, width are 160 mm, a height of 600 mm；The production hydrogen-based matter room 28
A length of 500 mm, width are 130 mm, a height of 600 mm；25 radius of feed inlet is 60 mm；The rectangular box,
One partition, second partition are made of organic glass.
Included the following steps: using the production hydrogen methods of above-mentioned combined reactor
1. it is raw to be separately added into the photosynthetic bacteria prepared in proportion into grown cultures basal cell 26 and clean water chamber 27 by feed inlet 25
Long culture medium and clear water connect 24 power supply of peristaltic pump, are delivered to photosynthetic bacteria cultivation region 8 and heat preservation lighting area 9 respectively, connect
3 power supply of LED light strip 16 and electric heating wire cultivates 48 h.
2. production hydrogen-based qualitative response liquid is added to is produced from hydrogen-based matter room 28 by feed inlet 25；Connect 24 power supply of peristaltic pump, Xiang Guang
Fermentation and hydrogen production area 10, which is added, produces hydrogen-based qualitative response liquid；5 power supply of solenoid valve is connected, from photosynthetic bacteria cultivation region 8 to light fermentation and hydrogen production
The conveying of area 10 photosynthetic bacteria, the 20 ~ 30% of 10 volume of volume Zhan Guang fermentation and hydrogen production area of photosynthetic bacteria；Hydrogen-based qualitative response liquid is produced to account for
The 70 ~ 80% of 10 volume of light fermentation and hydrogen production area；By the photosynthetic bacteria for having been transferred to light fermentation and hydrogen production area 10 and produce hydrogen-based qualitative response
Liquid is mixed, and photosynthetic bacteria carries out light using the reduced sugar produced in hydrogen-based qualitative response liquid under the illumination effect of LED light strip 16
It closes and produces hydrogen.Then growth medium is added to photosynthetic bacteria cultivation region 8, inoculation photosynthetic bacteria continues to cultivate, to use next time.
3. the hydrogen generated converges to the top in light fermentation and hydrogen production area 10 under the action of buoyancy, and from the production hydrogen of setting
The outlet of outlet conduit 13 exports, and hydrogen container is arranged producing gas outlet, for storing the hydrogen generated, by gas flowmeter 14
Record produces air volume.
4. the hydraulic retention time that system is arranged is 48 h, gas production rate is improved using the mode of intermittent stirring, every 4 h is stirred
Mix it is primary, when stirring a length of 30 min.
In the present embodiment, photosynthetic bacterium growth culture medium added by step 1 forms are as follows: distilled water, yeast extract 0.5
G/L, NaCl 1.0 g/L, MgSO4·7H2O 0.1 g/L, CH3COONa 2.0 g/L, K2HPO40.1 g/L, NaHCO3 1.0
G/L, NH4Cl 0.5 g/L.In photosynthetic bacteria cultivation region, the additive amount of 8 photosynthetic bacteria is 8 volume of photosynthetic bacteria cultivation region
40%, the volume of addition photosynthetic bacterium growth culture medium is the 60% of 8 volume of photosynthetic bacteria cultivation region.
The photosynthetic bacteria that step 1 is added, to be inoculated with the photosynthetic bacteria in logarithmic phase later period, regulating illumination intensity is
3000lx, temperature are cultivated under the conditions of being 30 DEG C.
In the present embodiment, the HAU-M1 photosynthetic hydrogen production bacterium that photosynthetic bacteria strain is provided from Agricultural University Of He'nan, with
Quantity is than meter, by 9% hydrogenlike silicon ion (Rhodobacter sphaeroides), 27% Rhodospirillum rubrum
(Rhodospirillum rubrum), 11% Rhodobacter capsulatus (Rhodobacter capsulatus), the 28% red vacation in marsh
Monad (Rhodopseudomonas pulastris) and 25% capsula Rhodopseudomonas (Rhodopseudomonas
Capsulata it) forms.
In the present embodiment, the composition of hydrogen-based qualitative response liquid is produced are as follows: by sodium glutamate, NaCl, K2HPO4, yeast extract,
MgCl2、NH4Cl, biomass straw powder, cellulase are added in buffer, make 3.56 g/L of concentration of sodium glutamate, NaCl
Concentration is 2.0 g/L, K2HPO4Concentration is 0.50 g/L, and yeast extract concentration is 0.10 g/L, MgCl2Concentration is 0.20 g/
L, NH4Cl concentration is 0.40 g/L, and cellulase is added according to 100mg/g stalk, and biomass straw powder has according to 25g/L
Volume addition is imitated, is uniformly mixed, effective volume is produces hydrogen-based qualitative response liquid product and added the sum of photosynthetic bacteria volume.
Buffer: buffer is the citric acid-sodium citrate solution of PH=4.8, and specific process for preparation is as follows:
Solution A: accurately weighing 21.014 g of citric acid in 500 mL beakers, after the dissolution of a small amount of distilled water, is settled to 1000
ML obtains 0.1 mol/L citric acid solution.
B solution: accurately weighing 29.412 g of sodium citrate in 500 mL beakers, after the dissolution of a small amount of distilled water, constant volume
To 1000 mL, 0.1 mol/L sodium citrate solution is obtained.
230 mL of solution A, 270 mL of B solution is taken to move into after being sufficiently mixed in 1000 mL volumetric flasks and be settled to distilled water
1000 mL, mix well.
The 20 ~ 30% of 10 volume of volume Zhan Guang fermentation and hydrogen production area of photosynthetic bacteria；It produces hydrogen-based qualitative response liquid and accounts for light fermentation and hydrogen production
The 70 ~ 80% of 10 volume of area can need to be adjusted according to actual experiment.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications done without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included in protection scope of the present invention.
Claims (9)
1. a kind of photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor, which is characterized in that including the cylinder being set on the first support
Body and the cabinet on the second support, cylindrical side wall group of the cylinder by top plate and bottom plate and between bottom plate and top plate
At, the first cylindrical partition and the second cylinder are successively arranged in cylinder from outside to inside and is separated, cylindrical side wall and the first cylinder
Photosynthetic bacteria cultivation region is formed between shape partition, forms heat preservation illumination between the first cylindrical partition and the second cylindrical partition
Area forms light fermentation and hydrogen production area in the second cylindrical partition, be successively arranged from left to right in the cabinet first partition, second every
Plate, the space between first partition and cabinet left side wall form grown cultures basal cell, the space between first partition, second partition
Clean water chamber is formed, the space between second partition and cabinet right side wall, which is formed, produces hydrogen-based matter room, and grown cultures basal cell passes through hose
It is connected to photosynthetic bacteria cultivation region, clean water chamber is connected to heat preservation lighting area by hose, is produced hydrogen-based matter room and is sent out by hose and light
The connection of the area Jiao Chanqing, photosynthetic bacteria cultivation region, heat preservation lighting area are equipped with temperature sensor, three temperature in light fermentation and hydrogen production area
Degree sensor is connected with temperature controller respectively.
2. photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor according to claim 1, which is characterized in that the second cylinder
Rabbling mechanism is equipped in shape partition, the rabbling mechanism includes motor, paddle and agitating shaft, and motor is set on top plate, stirring
Axis passes through top plate and top is connected with motor, and agitating shaft extends downwardly the lower part for protruding into light fermentation and hydrogen production area, and paddle is fixed on
On agitating shaft, the outer wall of the second cylindrical partition is equipped with light source, and top plate corresponding to light fermentation and hydrogen production area, which is equipped with, produces hydrogen outlet
Pipeline produces hydrogen outlet conduit and is equipped with gas flowmeter, keeps the temperature bottom plate corresponding to lighting area and is equipped with electric heating wire, photosynthetic thin
The lower part in bacterium cultivation region and light fermentation and hydrogen production area is equipped with communicating pipe, communicating pipe is equipped with solenoid valve；It is equipped with and cuts on every hose
Only valve and peristaltic pump.
3. photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor according to claim 1, which is characterized in that grown cultures
Basal cell, clean water chamber produce the top of hydrogen-based matter room equipped with feed inlet, grown cultures basal cell, clean water chamber, the lower part for producing hydrogen-based matter room
Not She You sample outlet pipe road, sample outlet pipe road is equipped with sample output valve, cylindrical side wall, the first cylindrical partition and the second cylindrical partition
Lower part be equipped with discharge pipe, discharge pipe is equipped with discharge valve.
4. photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor according to claim 1, which is characterized in that cylinder, first
Cylinder partition, the second cylindrical partition, cabinet, first partition, second partition are transparent material, the photosynthetic bacteria culture
Area, heat preservation lighting area, its volume of light fermentation and hydrogen production area are denoted as V respectively1 、V2、V3；And V2:V1>=1, V1:V3>=1:2, growth training
It supports basal cell, clean water chamber, produce hydrogen-based matter room, volume is respectively V4、V5、V6；The volume in the correspondence area that each building volume communicates therewith it
Than being equal to or more than 6:5, i.e. V4: V1>=6:5, V5: V2>=6:5, V6: V3≥6:5。
5. carrying out the side of hydrogen manufacturing using any photosynthetic bacteria culture-light fermentation and hydrogen production combined reactor of Claims 1-4 4
Method, which comprises the following steps:
A: the photosynthetic bacterium growth culture prepared in proportion is separately added into grown cultures basal cell and clean water chamber by feed inlet
Base and clear water connect wriggling pumping source, are delivered to photosynthetic bacteria cultivation region and heat preservation lighting area respectively, connect light source and electric heating
Silk power supply, cultivates 48 ~ 72h；
B: production hydrogen-based qualitative response liquid is added to is produced from hydrogen-based matter room by feed inlet；Wriggling pumping source is connected, produces hydrogen to photo fermentation
Area, which is added, produces hydrogen-based qualitative response liquid；Power of electromagnetic valve is connected, it is photosynthetic thin from photosynthetic bacteria cultivation region to the conveying of light fermentation and hydrogen production area
Bacterium；
C: hydrogen container is set in the exit for producing hydrogen outlet conduit, is recorded by gas flowmeter and produces air volume；
D: valve is closed, improves gas production rate using the mode of intermittent stirring.
6. hydrogen production process according to claim 5, which is characterized in that the photosynthetic production of HAU-M1 that the photosynthetic bacteria strain is
Hydrogen bacteria, in terms of quantity ratio, HAU-M1 photosynthetic hydrogen production bacterium by 9% hydrogenlike silicon ion, 27% Rhodospirillum rubrum, 11% pod
The red bacterium of film, 28% Rhodopseudomonas palustris and 25% capsula Rhodopseudomonas composition.
7. hydrogen production process according to claim 5, which is characterized in that the concrete composition of photosynthetic bacterium growth culture medium: ferment
Female 0.5 g/L of cream, NaCl 1.0 g/L, MgSO4·7H2O 0.1 g/L, CH3COONa 2.0 g/L, K2HPO40.1 g/L,
NaHCO31.0 g/L, NH40.5 g/L of Cl, surplus are distilled water.
8. hydrogen production process according to claim 5, which is characterized in that produce the composition of hydrogen-based qualitative response liquid are as follows: by glutamic acid
Sodium, NaCl, K2HPO4, yeast extract, MgCl2、NH4Cl, biomass straw powder, cellulase are added in buffer, make glutamic acid
Na concn is 3.56 g/L, and NaCl concentration is 2.0 g/L, K2HPO4Concentration is 0.50 g/L, and yeast extract concentration is 0.10 g/
L, MgCl2Concentration is 0.20 g/L, NH4Cl concentration is 0.40 g/L, and cellulase is added according to 100mg/g stalk, biomass
Stalk powder is added according to 25g/L effective volume, is uniformly mixed, and effective volume is produces hydrogen-based qualitative response liquid product and added
Add the sum of photosynthetic bacteria volume；Wherein buffer is the citric acid-sodium citrate solution of PH=4.8.
9. hydrogen production process according to claim 5, which is characterized in that the photosynthetic bacteria cultivation region and light fermentation and hydrogen production area
Reaction temperature be 30 ± 1 DEG C；The intensity of illumination of the LED light strip is 2500 ~ 3500 luxs.
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