CN105316217A - Artificial light source microalgae culture equipment - Google Patents
Artificial light source microalgae culture equipment Download PDFInfo
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- CN105316217A CN105316217A CN201510189268.9A CN201510189268A CN105316217A CN 105316217 A CN105316217 A CN 105316217A CN 201510189268 A CN201510189268 A CN 201510189268A CN 105316217 A CN105316217 A CN 105316217A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
Abstract
The invention discloses artificial light source microalgae culture equipment which comprises a reaction container, a feeding and discharging device, a light emitting device, a nutrition distributing device and a gas distributor. A cover plate is arranged on the reaction container which contains culture solution for microalgae growth, microalgae is pumped into or out of the reaction container through the feeding and discharging device, the light emitting device is used for generating a light source needed for microalgae growth, the nutrition distributing device is used for providing nutrition needed for microalgae growth into the reaction container, and the gas distributor is used for providing gas needed by microalgae growth into the reaction container. An infrared light source is arranged in the artificial light source microalgae culture equipment, a proper amount of manganese ions are provided in the culture liquid, and a proper amount of carbon dioxide is fed, so that microalgae culture efficiency is improved greatly. The artificial light source microalgae culture equipment is high in controllability and conducive to realizing stable and continuous industrialized production.
Description
Technical field
The present invention relates to biological technical field, be specifically related to source of artificial light both culturing microalgae equipment.
Background technology
Microalgae biomass is the future thrust of biomass economy, has the advantages that energy conversion efficiency is high, and compared with common Lu Sheng farm crop, the productive rate of unit surface can exceed decades of times, thus can realize industrialized culture.The ultimate principle of micro algae growth utilizes micro-algae photosynthesis, carbon dioxide conversion is the biomass of micro-algae self thus secures carbon, and by the biomass such as characteristic and function Synthetic Oil, protein, polysaccharide, Mierocrystalline cellulose of micro-algae self.Utilize physics or chemical process that the useful matter in microalgae cell is transformed into extracellular, then carry out refinement processing, thus produce the product such as biofuel, algal polysaccharides.Thus, in actual production, namely by the photosynthesis of algae, be the product that biofuel, protein etc. have high utility value by the nutritive substance in waste water and gas and carbon dioxide conversion.Significantly rise in oil price, today that food shortage problem is outstanding day by day, this industry has vast potential for future development.
For improve further microalgae biodiesel unit output, realize three dimensional-breeding, special both culturing microalgae photoreactor must be used.The outdoor direct cultivation such as raceway pond, multistage pond although both culturing microalgae photoreactor of the prior art is compared, cultivation density, yield per unit are all significantly improved, but because light source used when micro algae growth and working substance directional induction are produced is still natural light, micro-algae is insufficient to the utilization of sunlight, there is uncertain and uncontrollability, the requirement stablize with factorial praluction, continued runs in the opposite direction completely, in addition the auxiliary breeding function also unrealized automatization such as nutritional supplementation, carbonic acid gas inflation, makes itself and actual commercial application still have larger distance.
Therefore, current this area can overcome poor controllability, low, the inefficient drawback of level of automation of above-mentioned both culturing microalgae photoreactor of the prior art in the urgent need to a kind of technology.
Summary of the invention
The object of this invention is to provide a kind of can equipment according to the source of artificial light both culturing microalgae of the key element such as light source, carbonic acid gas, nutrition, temperature, flow velocity of cultivation demand proportioning the best of different sorts algae different steps, thus realize the stable, controlled of super-high density both culturing microalgae, meet industrialization demand.
For achieving the above object, the invention provides a kind of source of artificial light both culturing microalgae equipment, described source of artificial light both culturing microalgae equipment comprises:
Reaction vessel, described reaction vessel is provided with cover plate and described reaction vessel holds the nutrient solution supplying micro algae growth;
Charging/discharging device, described charging/discharging device and described reaction vessel are tightly connected;
Light-emitting device, described light-emitting device is located at reaction vessel interior and when the work of described reactor, described light-emitting device is immersed in described nutrient solution at least partly or all, thus the light needed for micro algae growth is provided in reaction vessel, the light intensity of the light that wherein said light-emitting device sends is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel;
Nutrition distribution device, described nutrition distribution device can provide mn ion in described nutrient solution; With
Air feeder, described air feeder is used for the gas provided in described reaction vessel needed for micro algae growth.
In a preferred embodiment, the mn ion source in described nutrient solution is from MnCl
24H
2o, and the MnCl added in often liter of described nutrient solution
24H
2the quality of O is 5-50mg.
In a preferred embodiment, during the work of described light-emitting device, launch the light of at least three different wave lengths, comprising wavelength be the light of 600-680nm, wavelength is the light of 420-500nm, and wavelength is the light of 700-780nm; And/or
The colour temperature of the complex light that described luminescence unit sends is 1000-20000K, preferably 1500-6000K.
In a preferred embodiment, the light of described three different wave lengths comprises light that wavelength is 650-670nm, wavelength is the light of 440-460nm, and wavelength is the light of 710-730nm.
In a preferred embodiment, described light-emitting device comprises light guiding plate and luminescence unit, described luminescence unit is embedded in described light guiding plate, the light transmission light guiding plate conduction that described luminescence unit produces makes light guiding plate Integral luminous, described light-emitting device also comprises support, described support is used for light guiding plate described in fixed support, and described support is removably connected with described reaction vessel and/or described cover plate; And/or
In described light-emitting device, luminescence unit is LED, and the quantity of LED is 1-10000/ light guiding plate; It is preferably 10-1000/ light guiding plate.
In a preferred embodiment, described air feeder can pass into the mixture of carbonic acid gas and air in described nutrient solution, and wherein carbonic acid gas accounts for the 0.1-10% of mixed gas cumulative volume, preferably accounts for 0.5-5%; And/or
It is 0.1-1MPa that carbonic acid gas passes into pressure; And/or
It is 0.1-50nm that carbonic acid gas disengages aperture.
In a preferred embodiment, the monitoring for carbonic acid gas intake in nutrient solution described in detection and control and automation control system is also provided with in described cultivation equipment, wherein
When described monitoring and automation control system detect pH >=5.5 in described nutrient solution, carbonated mixed gas is continued to pass in described nutrient solution; And as PH<5.5 in described nutrient solution, suspend in described nutrient solution and pass into carbonic acid gas.
In a preferred embodiment, the surrounding of described reaction vessel is provided with temperature control unit, and described temperature control unit is in the scope of applicable micro algae growth for the liquid environment temperature maintained in reaction vessel; And/or
Described air feeder comprises gas refinement sparger, is passing in the process of gas in reaction vessel, and the gas with certain pressure loose to be overflowed by the aperture of this refining device is all even and fine, thus impels gas and nutritive substance to be scattered in liquid culture system.
In a preferred embodiment, be also provided with the water quality monitoring system for monitoring mn ion or other ionic concns in described nutrient solution in described source of artificial light both culturing microalgae equipment, the intake of described water quality monitoring system is positioned at below liquid level, wherein
When the concentration that described water quality monitoring system monitors mn ion in described nutrient solution or other ions is lower than default value, described water quality monitoring system can send prompting, makes described nutrition distribution device carry out the interpolation of manganese salt or other nutritive salt.
In a preferred embodiment, described source of artificial light both culturing microalgae equipment is applicable to the cultivation of the fresh water eukaryotic algae such as chlorella, grid algae, Haematocoocus Pluvialls.
In a preferred embodiment, the inoculum density of the algae cultivated is 0.1g/L-2g/L (algae dry weight/nutrient solution volume), more preferably 0.2g/L-0.6g/L.
In a preference, the light intensity of the light that light-emitting device sends is substantially even in the horizontal direction (comprising X-axis and Y direction) of reaction vessel.
In a preference, described " uniformly or roughly uniform " refers to the light intensity D1 of arbitrary depth and meets following formula at the average intensity Dm of entire depth scope:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8; More preferably 1.1 >=D1/Dm >=0.9.
In a preference, the surrounding of reaction vessel is provided with temperature control unit, and this temperature control unit is in the scope of applicable micro algae growth for the liquid environment temperature maintained in reaction vessel.
In a preference, the cultivation temperature in this cultivation equipment is 10-30 DEG C, is more preferably 20-30 DEG C.
In a preference, this temperature control unit can comprise electrically heated or steam heating system, cold water cooling system etc., such as temperature control water pipe.
In a preference, source of artificial light both culturing microalgae equipment is also provided with Monitoring systems, and for the parameter of monitoring liquid environment, parameter is selected from: pH value, temperature and/or nutriment concentration.
In a preference, be on the cover board provided with production well.
In a preference, in this source of artificial light both culturing microalgae equipment, be provided with polylith light guiding plate, preferably 30-1000 block, more preferably 40-800 block, best 50-500 block.
In a preference, light guiding plate is made up of the transparent organic material with anti-weak acid performance.
Accompanying drawing explanation
Fig. 1 is the three-dimensional cutaway view of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 2 is the perspective cross-sectional schematic diagram of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 3 is that the master of source of artificial light both culturing microalgae equipment according to an embodiment of the invention looks cross-sectional schematic;
Fig. 4 be according to an embodiment of the invention source of artificial light both culturing microalgae equipment overlook cross-sectional schematic;
Fig. 5 is the schematic perspective view of the light-emitting device of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 6 a is the front view of the light-emitting device of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 6 b is the side-view of the light-emitting device of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 6 c is the vertical view of the light-emitting device of source of artificial light both culturing microalgae equipment according to an embodiment of the invention;
Fig. 6 d is the enlarged view according to part B in Fig. 6 b;
Fig. 7 a-7c is the simplification schematic top plan view of the artificial equipment for microalgae cultivation according to other embodiments of the invention; And
Fig. 8 is the representative side section view of the artificial equipment for microalgae cultivation according to other embodiments of the invention.
Embodiment
The present inventor is through extensive and deep research, develop a kind of source of artificial light both culturing microalgae equipment first, by structures such as the light-emitting devices of particular design, reactor of the present invention not only shortens incubation time, and the biomass density of micro-algae and total working substance content can be significantly improved, thus the better biomass material of quality and product can be prepared.Complete the present invention on this basis.
Below with reference to accompanying drawing, preferred embodiment of the present invention is described in detail, so that clearer understanding objects, features and advantages of the present invention.It should be understood that embodiment shown in the drawings is not limitation of the scope of the invention, and the connotation just in order to technical solution of the present invention is described.
Bio-reactor
Built-in light source bio-reactor source of artificial light both culturing microalgae equipment of the present invention is applicable to the high-density industrialization cultivation of the fresh water eukaryotic algae such as chlorella, grid algae, Haematocoocus Pluvialls.
As depicted in figs. 1 and 2, described source of artificial light both culturing microalgae equipment comprises reaction vessel 1, light-emitting device 2, charging/discharging device 3, cover plate 5, gas distributor 6, nutrition distribution device, temperature control unit 7 and monitor and forecast system etc.Reaction vessel 1 is provided with cover plate 5, reaction vessel 1 and charging/discharging device 3 are tightly connected, and charging/discharging device is provided with valve and switch, for controlling the carrying out of input and output material or stopping, light-emitting device 2 is provided with for generation of the stabilized light source needed for micro algae growth each stage in reaction vessel 1, the bottom of reaction vessel 1 is provided with gas distributor 6 for passing into the gas needed for micro algae growth each stage, nutrition distribution device is used for supplying nutrients in reaction vessel, the surrounding of reaction vessel is provided with temperature control unit 7 (being illustrated as temperature control water pipe) for keeping the temperature of solution in reaction vessel in the temperature range being applicable to the breeding of micro-algae quantity and individual growth.
The bottom (height of according to appointment 30% ~ 90%, or 50% ~ 70%) of this bio-reactor can be embedded in underground.
This bio-reactor can be arranged on around power station, and the waste water and gas utilizing generating to produce is originated as the matter and energy of both culturing microalgae, has the effect of environmental emission reduction.This reaction vessel 1 can be lighttight encloses container, also can carry out luminescence by the light-emitting device of self overcast and rainy with night, used for micro algae growth.In this cultivation equipment, being provided with monitoring and automation control system, for monitoring water temperature, pH value, CO2 concentration, TDS, absorbancy, light intensity etc., and Automated condtrol can being carried out to above-mentioned condition as required.
Fig. 3 is that the master of this source of artificial light both culturing microalgae equipment looks cross-sectional schematic; As shown in Figure 3, be provided with temperature control unit 7 on the walls of the reaction vessel, temperature control unit 7 can be any suitable form, comprises electrically heated or steam heating system, cold water cooling system, such as temperature control spacer etc.Preferably, temperature control unit 7 is temperature control water pipe, and the waste water with certain temperature that power station is discharged is used in temperature control water pipe and circulates to keep temperature in bio-reactor between 20 DEG C ~ 30 DEG C.
Production well is provided with, for discharging the oxygen that micro-algae photosynthesis produces at the top of the cover plate 5 of bio-reactor.
Fig. 4 be this source of artificial light both culturing microalgae equipment overlook cross-sectional schematic.As shown in the figure, be provided with gas distributor 6 in the bottom of source of artificial light both culturing microalgae equipment, gas distributor 6 can be rotary, slot type, tubular type or other any suitable forms.In microalga cultivation process, in reaction vessel, pass into carbonic acid gas, air or other gas by gas distributor.Preferably, adopt rotary gas sparger in this example, in the process passing into gas, gas distributor 6 rotarily drives the disturbance of liquid in the gas and bottom container that pass into, thus is conducive to gas and nutritive substance is uniformly distributed in the solution.
In one or more positions (on such as wall of container, inside container bottom, container top and cover plate etc.) of reaction vessel, nutrition distribution device is installed.Nutrition distribution device provides different nutriment in the different steps of micro algae growth.
In reaction vessel, other nutritive substances passes into speed and CO
2pass into the ratio that speed can become certain, pass through CO
2on-line monitoring system can determine CO in solution
2meltage, know the potential of hydrogen of solution by the pH value of Monitoring systems monitoring solution simultaneously.The CO that Controlling System can control
2with nutritive substance pass into speed, ensure that the meltage of solution Middle nutrition material and pH value are in the scope of applicable micro algae growth breeding or produce oil.
Reaction vessel of the present invention can adopt glass reinforced plastic to be made, and reaction vessel can have any suitable profile and size.Vessel surface is more smooth, and preferably, vessel surface has moveable closed cover plate.
As shown in Fig. 7 a-7c, this reaction vessel can be long annular stereo container.The total length of this container is 2m-500m, is preferably 10m-200m; Container section depth-width ratio is 0.5:1-1:0.5; Be highly 20cm-400m, preferably for being 50cm-200cm.Preferably, as shown in Figure 8, in the middle of the bottom section of container, low both sides are high, and lower-most point is positioned at center, and bottom both sides, gradient is 5 °-60 °, is more preferably 10 °-30 °.
Container turns to closed cylinder at the section-variable of middle portion, and drum diameter is 20cm-400m, and be more preferably 50cm-200cm, this partial-length can be 20cm-100cm, more preferably can be 20cm-50cm.The conversion portion of the different cross section shape of container is in being smoothly connected.
Cultivation equipment of the present invention also comprises other air feeders, such as not shown source of the gas, mixing tank, pneumatic tube etc.In the present invention, source of the gas is mainly air and carbonic acid gas.Mixing tank is mainly used in mixing air and carbonic acid gas, and mixing tank can adopt Venturi tube or static mixer.Blowing mouth material can be ceramic capillary or resin microvoid structure, and micro-pore diameter is 0.1-10 micron, is more preferably 0.5-5 micron.Gas distributor is arranged at bottom Cultivation container, end 0-5cm in the lower end distance container of gas distributor.As shown in the figure, reaction container bottom can be provided with multiple gas distributor arranged at equal intervals side by side.During use, the supply gas pressure of gas distributor is 0.2-20Mpa, is more preferably 0.5-5Mpa.
Preferably, the nutrition distribution device in cultivation equipment of the present invention can be the perforated tube being positioned at upper vessel portion, and nutritive medium evenly can be sprayed to through aperture in the nutrient solution in container after above pipeline flow to container.In addition, contriver is through experiment and probe into, and finds in nutrient solution, provide a certain amount of mn ion, effectively can improve culture benefit.Therefore, the nutrition distribution device of cultivation equipment of the present invention can provide mn ion in nutrient solution.
Preferably, cultivation equipment of the present invention is also provided with circulation puopulsion unit, and circulation puopulsion unit can have the propelling blade of propeller type or screw, for flowing and the circulation of water body in system.Advancing blade rotating speed to be 10-1000rpm, is more preferably 20-400rpm.
In addition, cultivation equipment of the present invention also can integrate the online water quality monitoring system for detecting manganese ion concentration in nutrient solution, so that the manganese ion concentration controlled in nutrient solution reaches the scope being conducive to both culturing microalgae most.The water sample intake of this water quality monitoring system is positioned at below liquid level, can timing automatic sampling.Through microfiltration system before water sample sends into detector automatically, filtering accuracy is T level.When monitoring mn ion content lower than 5mg/L (or numerical value of other settings), system is automatically opened nutritive salt and is added or remind managerial personnel to carry out nutritive salt feed supplement.
The pH scope of the nutrient solution in cultivation equipment of the present invention is 5-10, is more preferably 6-8.Cultivation equipment of the present invention also can integrate PH on-line monitoring sensor, for measuring the pH value in nutrient solution, to control the concentration passing into carbonic acid gas in nutrient solution.When pH in nutrient solution is higher than 6.5 (or numerical value of other settings), system opens carbonic acid gas valve automatically, and Xiang Chizhong passes into carbon dioxide; When ph is lower than 6.5 (or numerical value of other settings), system closing carbonic acid gas valve, breather only passes into air or directly stops passing into gas in pond, thus the intake of effective regulation of carbon dioxide, to obtain the best carbon dioxide conditions of micro-algae propagation and growth.
Light-emitting device
Fig. 5 is the schematic perspective view of light-emitting device 2.Light-emitting device be arranged so that bio-reactor can when carrying out the normal operations such as breeding production without when sunlight or other external light sources.As described in Figure 5, described light-emitting device is made up of support 9, LED luminescence unit group 10 and light guiding plate 11, and light guiding plate 11 is fixed by support 9, and the top of support 9 is fixed on cover plate 5, LED luminescence unit group 10 is positioned at the top of light guiding plate 11, light guiding plate 11 or all stretch under liquid level.In bio-reactor of the present invention, be provided with multiple light-emitting device 2, each light guiding plate all can install or remove individually.
Light guiding plate can be made with transparent organic material (such as acrylic), and has anti-weak acid performance.LED luminescence unit group directly contacts with light guiding plate at the top of light guiding plate, and the light that LED luminescence unit group sends can pass through light guiding plate and conducts and make light guiding plate Integral luminous.Preferably, the parallel placement of light guiding plate, the distance between two boards, for can be 5cm-50cm, more preferably, is 10cm-20cm.Luminescent screen illumination can adjust as required.
The top of the support 9 of light-emitting device 2 is removably connected with cover plate 5, thus be convenient to light-emitting device dismounting, change or reinstall; Light-emitting device 2 is suspended on the bottom of not contacting container in reaction vessel, does not thus affect the rotation of container bottom gas distributor.Should be understood that the support of light-emitting device also can be fixed in any suitable position and reaction vessel in other any suitable modes.
Fig. 6 a-6c is the front view of light-emitting device of source of artificial light both culturing microalgae equipment, side-view and vertical view respectively; Fig. 6 d is the enlarged view of part B in Fig. 6 b.
LED luminescence unit group 10 comprises LED luminescence unit little one by one, and the light that luminescence unit produces can be tertiary colour or monochromatic ray, and monochromatic LED emission wavelength range is 350-900nm, and complex light reference color temperature is 1500-20000K.The quantity of the LED luminescence unit on single light guiding plate can be 1-10000.
Light source is LED light source
The light of different wave length can be launched during light-emitting device work.The light of this different wave length comprises: wavelength is: 600-680nm, more preferably the light A of 650-670nm; Wavelength is 420-500nm, more preferably the light B of 440-460nm; 700-780nm, more preferably the light C (infrared light) of 710-730nm.
Contriver through Experimental Research deep for a long time, find light-emitting device send the proportioning of the light of different wave length when following scope, the both culturing microalgae benefit in cultivation equipment can reach higher level.Contriver found through experiments, and when same illumination power, after supplementing the near infrared light of specific wavelength, can make micro-algae output increased 10-25%:
A:B＝10:1～2:1；
A:C＝20:1～3:1。
In addition, the intensity of illumination of light-emitting device can adjust, during illumination bright dark alternately (luminous power change).Preferably, when during half-light, luminous power is light, the 0%-80% of luminous power is more preferably 0%-60%.Preferably, half-light irradiation time is the 10%-300% more preferably 20%-100% of bright light exposure time
Wherein the light intensity of light that sends of light-emitting device is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel; The light intensity of the light that light-emitting device sends is substantially even in the horizontal direction (comprising X-axis and Y direction) of reaction vessel." uniformly or roughly uniform " refers to the light intensity D1 of arbitrary depth and meets following formula at the average intensity Dm of entire depth scope:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8; More preferably 1.1 >=D1/Dm >=0.9.
In fact, LED luminescence unit group 10 can be positioned at the bottom of light guiding plate 11 or be embedded in other suitable positions of luminescent screen, makes monoblock light guiding plate luminous as long as ensure that its light sent can conduct in monoblock light guiding plate.
Preferably, the energy derive of LED luminescence unit group is the electric energy that sun power tabula rasa absorption sun power produces.So, though bio-reactor of the present invention does not directly utilize sun power, but by sun power tabula rasa, the solar energy collecting of instability is got up for generating, the electricity produced stably is supplied to the luminescence unit group of bio-reactor to make it luminous constantly, ensures stable, the persistence of both culturing microalgae.
Produce cultivating system
The quantity breeding of micro-algae and individual weight gain stage can carry out in same bio-reactor, but need the carrying out along with growth, change light-emitting device, and the kind that change nutritive substance passes into and speed, process is comparatively complicated.
In order to realize efficient large-scale farming, multiple bio-reactor serial or parallel connection can be adopted to form and to produce cultivating system on a large scale.Serial or parallel connection between bio-reactor is connected by feed inlet and outlet, can complete charging between bio-reactor and discharging by pump system.
Consider the number change in microalga cultivation process, the demand of light source and nutritive substance is also constantly being changed, the cultivating system that multiple bio-reactor is connected can be adopted, such as, adopt the system of three bio-reactor series connection to complete whole process of growth of micro-algae production:
Appropriate algae kind is put into first step bio-reactor, and breeding is increased to some amount, enters second stage bio-reactor carry out further growth breeding by feed inlet and outlet, then enter third stage bio-reactor again, no longer breed, only mainly carry out individual growth, and less breeding.
The light-emitting device that emission wavelength is only applicable to being conducive to micro-algae propagation is provided with in the first step and second stage bio-reactor, and nutrition distribution device passes into the oxynitride such as ammonium phosphate, potassium primary phosphate or dipotassium hydrogen phosphate as nitrogen phosphorus source in reaction vessel, add the necessary nutritive salt of other micro algae growths such as iron, zinc simultaneously, gas distributor passes into oxynitride and CO2 in reaction vessel, in the bio-reactor of the second stage luminous intensity and nutritive substance delivery rate large compared with first step bio-reactor, to adapt to the growth demand of breeding micro-algae of increasing; In third stage bio-reactor, be provided with the light-emitting device that emission wavelength is only applicable to being conducive to the growth of micro-phycobiont, PH and temperature etc. are adjusted to the condition of applicable micro-phycobiont growth simultaneously, and gas distributor passes into CO2 in reaction vessel.
Bio-reactor not at the same level can select different sizes according to demand, and the comparatively rear two-stage of the such as size of first step bio-reactor is less.
After micro-algae in first step bio-reactor is expelled to second stage bio-reactor, new algae kind can be passed into first step bio-reactor, carry out new round cultivation simultaneously.In order to improve the purity of algae kind, the micro-algae can discharged from second stage bio-reactor, filter out the algae kind of part as first step bio-reactor.
Cultural method
The invention provides a kind of method of cultivating micro-algae, the method comprising the steps of:
1. provide source of artificial light both culturing microalgae of the present invention to equip;
2. algae kind is invested in this source of artificial light both culturing microalgae equipment, there is provided in reactor micro-algae survive needed for nutriment (comprise nitrogenous source, phosphorus source, inorganic salt (such as manganese, iron, zinc, magnesium etc.) carbonic acid gas or air are provided, open light-emitting device and produce light needed for micro algae growth.
It is applicable to microorganism of the present invention be not particularly limited, as long as can utilize light source to grow.Representational microorganism comprises (but being not limited to): the micro-algae of Chlorophyta, Cyanophyta, Chrysophyta and rhodophyta.A kind of preferred microorganism is chlorella (Chlorellales) fresh water species.Cultivation equipment of the present invention and breeding technique are particularly useful for the cultivation of the fresh water eukaryotic algae such as chlorella, grid algae, Haematocoocus Pluvialls.
nutritive ingredient
In the reaction vessel of cultivation equipment of the present invention, the inoculum density of institute's cultivating microalgae is 0.1g/L-2g/L (microalgae dry weight/nutrient solution volume), more preferably 0.2g/L-0.6g/L.The supply of the nutritive ingredient that nutrition distribution device and air feeder provide and should be raised concentration and determine according to inoculation, suitably should improve nutrient concentration when inoculating and raising when concentration improves, and keeps in breeding process and the consumption of extra-nutrition salt.
Cultivation equipment of the present invention can pass into carbonic acid gas as carbon source.Preferably, carbonic acid gas and air mixed pass into, and wherein carbonic acid gas accounts for the 0.1-10% of mixed gas cumulative volume, are preferably 0.5-5%; It is 0.1-1MPa that carbonic acid gas passes into pressure; It is 0.1-50nm more preferably 1-20nm that carbonic acid gas disengages aperture.
The pH scope of the nutrient solution in cultivation equipment of the present invention is 5-10, is more preferably 6-8.Pass into two in order to what control carbonic acid gas in nutrient solution better, in a preferred embodiment, when pH >=5.5 in nutrient solution, sustainablely pass into carbonated mixed gas; And as PH<5.5, can ventilation be suspended.
Nitrogenous source in nutrient solution can select one or more of following nitrogenous compound, and in various often liter of nutrient solution, the intake of nitrogenous compound is roughly as follows:
CO(NH
2)
2，500-1500mg/L；
NaNO
3，1000-3000mg/L；
NH
4HCO
3，1000-3000mg/L；
NH
4NO
3，500-1500mg/L。
One or more of following P contained compound can be selected in phosphorus source in nutrient solution, and in various often liter of nutrient solution, the intake of P contained compound is roughly as follows:
K
2HPO
4，20-80mg/L；
KH
2PO
4，20-80mg/L。
Contriver is through long-term and deep research, and when finding to add appropriate mn ion in nutrient solution, cultivation efficiency promotes greatly.Such as, the MnCl24H2O that concentration is 5-50mg/L (weight of additive/nutrient solution volume) can be added in nutrient solution.Preferably, in the cultivating system that the substratum BG11 Medium Proportion that usual algae kind uses becomes, MnCl24H2O concentration is 1.86mg/L.
Except above-mentioned carbon source, nitrogenous source, phosphorus source, mn ion, usually add other nutritive salt following in nutrient solution, and the concentration range of other nutritive salt is as follows:
MgSO
4·7H
2O，70-150mg/L；
CaCl
2·2H
2O，30-80mg/L；
Lemon, 5-10mg/L;
Ironic citrate, 5-50mg/L;
H
3BO
3，1.5-5mg/L；
ZnSO
4·7H
2O，0.2-0.5mg/L；
Na
2MoO
4·2H
2O，0.2-0.8mg/L；
CuSO
4·5H
2O，0.05-0.3mg/L；
Co(NO
3)
2·6H
2O0.03-0.2mg/L。
Preparation method
The invention provides a kind of method preparing microalgae biomass, the method adopts the micro-algae algae liquid prepared by preceding method as raw material, processes, thus obtained micro-algae finished product, a kind of typical course of processing comprises step:
The method utilizing filtration, press filtration or bubble to suspend collects micro-algae, and oven drying at low temperature can obtain micro-algae finished product after then pulverizing.
Embodiment 1
In microlamp's experimental box, carry out micro-algae propagation, selected algae kind is Chlorella pyrenoidesa, and inoculum density is 0.3g/L (dry weight).Source of artificial light is three-color LED light source, wavelength is respectively 650 ± 5nm, 440 ± 5nm, 720 ± 10nm, three-color light source proportioning is 10:3:2, light most dark place light intensity is about 3000lx, and bright dark alt time is 10 hours (bright): 2 hours (secretly), and half-light power is about 40% of light power.Pass into carbonic acid gas, pressure is 0.4Mpa, and the aperture of the ceramic membrane penstock disengaged is 10nm, carbonic acid gas and air mixed accounting are 2%-3%, Continuous aeration stops to about PH=6.5, reduces carbonic acid gas and air mixed accounting, until PH interval remains between 6.5-6.8.Cultivation temperature remains 27 degree ± 1 degree.
In the present embodiment, the nutrition of nutrient solution is chosen as:
CO(NH
2)
2，800mg/L；
NaNO
3，1200mg/L；
KH
2PO
4，50mg/L；
MgSO
4·7H
2O，100mg/L；
CaCl
2·2H
2O，40mg/L；
Citric acid, 6mg/L;
Ferric ammonium citrate, 25mg/L;
H
3BO
3，3mg/L；
MnCl
2·4H
2O，12mg/L；
ZnSO
4·7H
2O，0.3mg/L；
Na
2MoO
4·2H
2O，0.4mg/L；
CuSO
4·5H
2O，0.1mg/L；
Co(NO
3)
2·6H
2O，0.06mg/L。
Monitor more than three times ionic concns every day, and suitably feed supplement keeps nutrition.
Cultivate after 7 days, weigh after collection, filtration, oven dry, obtaining final biomass concentration is 1.07g/L.
Comparative example 1:
Adopt same method, but MnCl
24H
2o concentration becomes 1.8mg/L, cultivates after 7 days, and weigh after collection, filtration, oven dry, obtaining final biomass concentration is 0.71g/L.
Comparative example 2:
Adopt same method, but do not add MnCl24H2O in substratum, cultivate after 7 days, weigh after collection, filtration, oven dry, obtaining final biomass concentration is 0.42g/L, and yellowish, there is floating dead algae.
Comparative example 3:
Adopt same method, but MnCl24H2O concentration becomes 100mg/L, cultivates after 7 days, weigh after collection, filtration, oven dry, obtaining final biomass concentration is 0.52g/L, occurs floating dead algae.
Comparative example 4:
Adopt same method, but source of artificial light is dichromatism LED light source, wavelength is respectively 650 ± 5nm, 440 ± 5nm, and dichromatism light source proportioning is 10:3, cultivates after 7 days, and weigh after collection, filtration, oven dry, obtaining final biomass concentration is 0.82g/L.
Comparative example 5:
Adopt same method, but do not pass into carbonic acid gas, only pass into air, cultivate after 7 days, weigh after collection, filtration, oven dry, obtaining final biomass concentration is 0.79g/L.
Comparative example 6:
Adopt same method, but obstructed any gas, frond generation sedimentation, cannot cultivate.
Conclusion
The experimental result of embodiment above and comparative example can be found out:
1. MnCl in embodiment 1
24H
2the concentration of O is 12mg/L, and cultivating the final biomass concentration obtained after 7 days is 1.07g/L, and in comparative example 1-3 or reduce or increase MnCl
24H
2the concentration of O, the final biomass concentration that discovery cultivation obtained after 7 days all comparatively embodiment 1 reduces to some extent, and there is floating dead algae in some comparative examples, this shows, add in nutrient solution appropriate mn ion to be really conducive in cultivation equipment cultivate the growth of algae;
2. in embodiment 1, adopt proportioning to be the three-color LED light source of 10:3:2, and in comparative example 4, have employed the dichromatism LED light source not launching infrared light, under the condition that other culture condition are identical, the cultivation achievement of embodiment 1 is obviously better than comparative example 4, this shows, a certain proportion of infrared source to be conducive in cultivation equipment cultivate the growth of algae;
3. in embodiment 1, pass into certain mixing carbonic acid gas of accounting and the mixed gas of air, and in comparative example 5, do not pass into carbonic acid gas, any gas is not passed in comparative example 6, as can be seen from cultivation achievement, pass into carbonic acid gas and the air of appropriate blending ratio, very necessary to the Growth and reproduction of algae.
The all documents mentioned in the present invention are quoted as a reference all in this application, are just quoted separately as a reference as each section of document.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a source of artificial light both culturing microalgae equipment, is characterized in that, described source of artificial light both culturing microalgae equipment comprises:
Reaction vessel, described reaction vessel is provided with cover plate and described reaction vessel holds the nutrient solution supplying micro algae growth;
Charging/discharging device, described charging/discharging device and described reaction vessel are tightly connected;
Light-emitting device, described light-emitting device is located at reaction vessel interior and when the work of described reactor, described light-emitting device is immersed in described nutrient solution at least partly or all, thus the light needed for micro algae growth is provided in reaction vessel, the light intensity of the light that wherein said light-emitting device sends is uniform or roughly uniform at the depth direction (Z-direction) of reaction vessel;
Nutrition distribution device, described nutrition distribution device can provide mn ion in described nutrient solution; With
Air feeder, described air feeder is used for the gas provided in described reaction vessel needed for micro algae growth.
2. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, the mn ion source in described nutrient solution is from MnCl
24H
2o, and the MnCl added in often liter of described nutrient solution
24H
2the quality of O is 5-50mg.
3. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, launch the light of at least three different wave lengths during the work of described light-emitting device, comprising wavelength be the light of 600-680nm, wavelength is the light of 420-500nm, and wavelength is the light of 700-780nm; And/or
The colour temperature of the complex light that described luminescence unit sends is 1000-20000K, preferably 1500-6000K.
4. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, described light-emitting device comprises light guiding plate and luminescence unit, described luminescence unit is embedded in described light guiding plate, the light transmission light guiding plate conduction that described luminescence unit produces makes light guiding plate Integral luminous, described light-emitting device also comprises support, and described support is used for light guiding plate described in fixed support, and described support is removably connected with described reaction vessel and/or described cover plate; And/or
In described light-emitting device, luminescence unit is LED, and the quantity of LED is 1-10000/ light guiding plate; It is preferably 10-1000/ light guiding plate.
5. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, described air feeder can pass into the mixture of carbonic acid gas and air in described nutrient solution, and wherein carbonic acid gas accounts for the 0.1-10% of mixed gas cumulative volume, preferably accounts for 0.5-5%; And/or
It is 0.1-1MPa that carbonic acid gas passes into pressure; And/or
It is 0.1-50nm that carbonic acid gas disengages aperture.
6. source of artificial light both culturing microalgae equipment as claimed in claim 1, is characterized in that, be also provided with the monitoring for carbonic acid gas intake in nutrient solution described in detection and control and automation control system, wherein in described cultivation equipment
When described monitoring and automation control system detect pH >=5.5 in described nutrient solution, carbonated mixed gas is continued to pass in described nutrient solution; And as PH<5.5 in described nutrient solution, suspend in described nutrient solution and pass into carbonic acid gas.
7. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, the surrounding of described reaction vessel is provided with temperature control unit, and described temperature control unit is in the scope of applicable micro algae growth for the liquid environment temperature maintained in reaction vessel; And/or
Described air feeder comprises gas refinement sparger, is passing in the process of gas in reaction vessel, and the gas with certain pressure loose to be overflowed by the aperture of this refining device is all even and fine, thus impels gas and nutritive substance to be scattered in liquid culture system.
8. source of artificial light both culturing microalgae equipment as claimed in claim 1, it is characterized in that, also be provided with the water quality monitoring system for monitoring mn ion or other ionic concns in described nutrient solution in described source of artificial light both culturing microalgae equipment, the intake of described water quality monitoring system is positioned at below liquid level, wherein
When the concentration that described water quality monitoring system monitors mn ion in described nutrient solution or other ions is lower than default value, described water quality monitoring system can send prompting, makes described nutrition distribution device carry out the interpolation of manganese salt or other nutritive salt.
9. source of artificial light both culturing microalgae equipment as claimed in claim 1, is characterized in that, described source of artificial light both culturing microalgae equipment is applicable to the cultivation of the fresh water eukaryotic algae such as chlorella, grid algae, Haematocoocus Pluvialls.
10. source of artificial light both culturing microalgae equipment as claimed in claim 9, it is characterized in that, the inoculum density of the algae cultivated is 0.1g/L-2g/L (algae dry weight/nutrient solution volume), more preferably 0.2g/L-0.6g/L.
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| PCT/CN2016/079498 WO2016165659A1 (en) | 2015-04-17 | 2016-04-15 | Artificially lighted microalgae farming apparatus |
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|---|---|---|---|---|
| WO2016165659A1 (en) * | 2015-04-17 | 2016-10-20 | 上海希明生物科技有限公司 | Artificially lighted microalgae farming apparatus |
| WO2016165660A1 (en) * | 2015-04-17 | 2016-10-20 | 上海希明生物科技有限公司 | Method for farming freshwater eukaryotic microalgae |
| WO2017190504A1 (en) * | 2016-05-05 | 2017-11-09 | 中国科学院广州能源研究所 | Rotating disc type photobioreactor for microalgae large-scale cultivation |
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| CN107227252A (en) * | 2017-07-03 | 2017-10-03 | 深圳市华明佰利科技有限公司 | A kind of vertical LED luminous energy biological respinse kettle of totally-enclosed |
| US20210246404A1 (en) * | 2018-05-03 | 2021-08-12 | Vaxa Technologies Ltd. | Device and method for storing live microalgae |
| CN114438071A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microalgae, preparation method thereof, culture pond and application |
| CN114438070A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microorganism, preparation method thereof, culture pond and application |
| CN114703051B (en) * | 2022-03-11 | 2024-03-29 | 湖南康大生物药品有限责任公司 | Diatom biological culture system and culture method |
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| CN105316217B (en) | 2019-05-03 |
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