CN110150528B - Effervescent tablet and preparation method and application thereof - Google Patents
Effervescent tablet and preparation method and application thereof Download PDFInfo
- Publication number
- CN110150528B CN110150528B CN201910409294.6A CN201910409294A CN110150528B CN 110150528 B CN110150528 B CN 110150528B CN 201910409294 A CN201910409294 A CN 201910409294A CN 110150528 B CN110150528 B CN 110150528B
- Authority
- CN
- China
- Prior art keywords
- parts
- extract
- extracting
- extraction
- mass
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007938 effervescent tablet Substances 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000000284 extract Substances 0.000 claims abstract description 161
- 239000004480 active ingredient Substances 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 23
- 244000298800 Actinidia arguta Species 0.000 claims abstract description 16
- 235000016416 Actinidia arguta Nutrition 0.000 claims abstract description 16
- 235000002722 Dioscorea batatas Nutrition 0.000 claims abstract description 16
- 235000006536 Dioscorea esculenta Nutrition 0.000 claims abstract description 16
- 240000001811 Dioscorea oppositifolia Species 0.000 claims abstract description 16
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 claims abstract description 16
- 241000371652 Curvularia clavata Species 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 15
- 240000001890 Ribes hudsonianum Species 0.000 claims abstract description 13
- 235000016954 Ribes hudsonianum Nutrition 0.000 claims abstract description 13
- 235000001466 Ribes nigrum Nutrition 0.000 claims abstract description 13
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 12
- 240000000851 Vaccinium corymbosum Species 0.000 claims abstract description 12
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims abstract description 12
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims abstract description 12
- 235000021014 blueberries Nutrition 0.000 claims abstract description 12
- 241000007126 Codonopsis pilosula Species 0.000 claims abstract description 11
- 241000112528 Ligusticum striatum Species 0.000 claims abstract description 11
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims abstract description 11
- 235000006545 Ziziphus mauritiana Nutrition 0.000 claims abstract description 11
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims abstract description 11
- 244000126002 Ziziphus vulgaris Species 0.000 claims abstract description 10
- 230000006870 function Effects 0.000 claims abstract description 10
- 240000000249 Morus alba Species 0.000 claims abstract description 9
- 210000004185 liver Anatomy 0.000 claims abstract description 9
- 230000002929 anti-fatigue Effects 0.000 claims abstract description 7
- 235000012907 honey Nutrition 0.000 claims abstract description 7
- 230000003064 anti-oxidating effect Effects 0.000 claims abstract description 4
- 230000002218 hypoglycaemic effect Effects 0.000 claims abstract description 4
- 240000004507 Abelmoschus esculentus Species 0.000 claims abstract 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 88
- 238000000034 method Methods 0.000 claims description 52
- 238000000605 extraction Methods 0.000 claims description 50
- 239000000243 solution Substances 0.000 claims description 37
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 30
- 239000002904 solvent Substances 0.000 claims description 27
- 235000019441 ethanol Nutrition 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000004108 freeze drying Methods 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 25
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 20
- 108010059892 Cellulase Proteins 0.000 claims description 18
- 229940106157 cellulase Drugs 0.000 claims description 18
- 230000002255 enzymatic effect Effects 0.000 claims description 18
- 229940059442 hemicellulase Drugs 0.000 claims description 18
- 108010002430 hemicellulase Proteins 0.000 claims description 18
- 239000007787 solid Substances 0.000 claims description 18
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 16
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 16
- 239000000811 xylitol Substances 0.000 claims description 16
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 16
- 235000010447 xylitol Nutrition 0.000 claims description 16
- 229960002675 xylitol Drugs 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 238000001694 spray drying Methods 0.000 claims description 14
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 239000011347 resin Substances 0.000 claims description 12
- 229920005989 resin Polymers 0.000 claims description 12
- 230000000415 inactivating effect Effects 0.000 claims description 11
- 239000000314 lubricant Substances 0.000 claims description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 10
- 241000245665 Taraxacum Species 0.000 claims description 10
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 10
- 230000008014 freezing Effects 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000000661 sodium alginate Substances 0.000 claims description 10
- 235000010413 sodium alginate Nutrition 0.000 claims description 10
- 229940005550 sodium alginate Drugs 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 10
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 9
- 239000000853 adhesive Substances 0.000 claims description 9
- 230000001070 adhesive effect Effects 0.000 claims description 9
- 239000000600 sorbitol Substances 0.000 claims description 9
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 7
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 6
- 239000008118 PEG 6000 Substances 0.000 claims description 6
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 claims description 6
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 6
- 229930003268 Vitamin C Natural products 0.000 claims description 6
- 239000002585 base Substances 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 6
- 235000019154 vitamin C Nutrition 0.000 claims description 6
- 239000011718 vitamin C Substances 0.000 claims description 6
- 108010059820 Polygalacturonase Proteins 0.000 claims description 5
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 239000004382 Amylase Substances 0.000 claims description 4
- 108010065511 Amylases Proteins 0.000 claims description 4
- 102000013142 Amylases Human genes 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 235000019418 amylase Nutrition 0.000 claims description 4
- 239000000469 ethanolic extract Substances 0.000 claims description 4
- 239000013538 functional additive Substances 0.000 claims description 4
- DKAGJZJALZXOOV-UHFFFAOYSA-N hydrate;hydrochloride Chemical compound O.Cl DKAGJZJALZXOOV-UHFFFAOYSA-N 0.000 claims description 4
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- 241000218231 Moraceae Species 0.000 claims description 3
- 210000001156 gastric mucosa Anatomy 0.000 claims description 3
- 241000756943 Codonopsis Species 0.000 claims description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims description 2
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims description 2
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 2
- 238000007908 dry granulation Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 235000019359 magnesium stearate Nutrition 0.000 claims description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 238000005469 granulation Methods 0.000 claims 1
- 230000003179 granulation Effects 0.000 claims 1
- 230000006641 stabilisation Effects 0.000 claims 1
- 238000011105 stabilization Methods 0.000 claims 1
- 208000007107 Stomach Ulcer Diseases 0.000 abstract description 9
- 201000005917 gastric ulcer Diseases 0.000 abstract description 9
- 230000000259 anti-tumor effect Effects 0.000 abstract description 8
- 240000001949 Taraxacum officinale Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 54
- 108090000790 Enzymes Proteins 0.000 description 24
- 102000004190 Enzymes Human genes 0.000 description 24
- 229940088598 enzyme Drugs 0.000 description 24
- 241000699670 Mus sp. Species 0.000 description 23
- 241000700159 Rattus Species 0.000 description 18
- 239000008280 blood Substances 0.000 description 18
- 210000004369 blood Anatomy 0.000 description 18
- 238000003304 gavage Methods 0.000 description 12
- 230000037396 body weight Effects 0.000 description 11
- 241001075517 Abelmoschus Species 0.000 description 10
- 235000010208 anthocyanin Nutrition 0.000 description 10
- 229930002877 anthocyanin Natural products 0.000 description 10
- 239000004410 anthocyanin Substances 0.000 description 10
- 150000004636 anthocyanins Chemical class 0.000 description 10
- 238000000465 moulding Methods 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 150000004676 glycans Chemical class 0.000 description 9
- 229920001282 polysaccharide Polymers 0.000 description 9
- 239000005017 polysaccharide Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 235000000346 sugar Nutrition 0.000 description 9
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 208000025865 Ulcer Diseases 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 230000002496 gastric effect Effects 0.000 description 8
- 210000002784 stomach Anatomy 0.000 description 8
- 231100000397 ulcer Toxicity 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 6
- 229930003944 flavone Natural products 0.000 description 6
- 150000002212 flavone derivatives Chemical class 0.000 description 6
- 235000011949 flavones Nutrition 0.000 description 6
- 150000003648 triterpenes Chemical class 0.000 description 6
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 230000037406 food intake Effects 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- ZHIBQGJKHVBLJJ-UHFFFAOYSA-N histamine phosphate Chemical group OP(O)(O)=O.OP(O)(O)=O.NCCC1=CNC=N1 ZHIBQGJKHVBLJJ-UHFFFAOYSA-N 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 229930182490 saponin Natural products 0.000 description 5
- 150000007949 saponins Chemical class 0.000 description 5
- 235000017709 saponins Nutrition 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 206010061218 Inflammation Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 4
- 238000011047 acute toxicity test Methods 0.000 description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 235000012631 food intake Nutrition 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- 230000001575 pathological effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 238000012449 Kunming mouse Methods 0.000 description 3
- 230000002292 Radical scavenging effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 102000004139 alpha-Amylases Human genes 0.000 description 3
- 108090000637 alpha-Amylases Proteins 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 150000001875 compounds Chemical group 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000035622 drinking Effects 0.000 description 3
- -1 flavonoid compound Chemical class 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 210000005228 liver tissue Anatomy 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002000 scavenging effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000009182 swimming Effects 0.000 description 3
- 229910002012 Aerosil® Inorganic materials 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 2
- 206010019375 Helicobacter infections Diseases 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 2
- 206010067125 Liver injury Diseases 0.000 description 2
- HDPISCIDKUMRDI-UHFFFAOYSA-M N(=O)[O-].[Na+].[N+](=O)([O-])[O-].[Al+3] Chemical compound N(=O)[O-].[Na+].[N+](=O)([O-])[O-].[Al+3] HDPISCIDKUMRDI-UHFFFAOYSA-M 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 208000008469 Peptic Ulcer Diseases 0.000 description 2
- 208000005718 Stomach Neoplasms Diseases 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 208000000718 duodenal ulcer Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 206010017758 gastric cancer Diseases 0.000 description 2
- 231100000753 hepatic injury Toxicity 0.000 description 2
- 229960001660 histamine phosphate Drugs 0.000 description 2
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000003908 liver function Effects 0.000 description 2
- 239000008176 lyophilized powder Substances 0.000 description 2
- 230000006996 mental state Effects 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 210000004877 mucosa Anatomy 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Substances OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 201000011549 stomach cancer Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- HOSAORYWYJOQNO-UHFFFAOYSA-N 2-phenylchromen-4-one;2-phenyl-2,3-dihydrochromen-4-one Chemical group O1C2=CC=CC=C2C(=O)CC1C1=CC=CC=C1.O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 HOSAORYWYJOQNO-UHFFFAOYSA-N 0.000 description 1
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 1
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 description 1
- 208000034991 Hiatal Hernia Diseases 0.000 description 1
- 206010020028 Hiatus hernia Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 description 1
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 240000000038 Ziziphus mauritiana Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000002318 cardia Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- WQLVFSAGQJTQCK-UHFFFAOYSA-N diosgenin Natural products CC1C(C2(CCC3C4(C)CCC(O)CC4=CCC3C2C2)C)C2OC11CCC(C)CO1 WQLVFSAGQJTQCK-UHFFFAOYSA-N 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- NWKFECICNXDNOQ-UHFFFAOYSA-N flavylium Chemical compound C1=CC=CC=C1C1=CC=C(C=CC=C2)C2=[O+]1 NWKFECICNXDNOQ-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 229960005375 lutein Drugs 0.000 description 1
- KBPHJBAIARWVSC-RGZFRNHPSA-N lutein Chemical compound C([C@H](O)CC=1C)C(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\[C@H]1C(C)=C[C@H](O)CC1(C)C KBPHJBAIARWVSC-RGZFRNHPSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- HZAXFHJVJLSVMW-UHFFFAOYSA-N monoethanolamine hydrochloride Natural products NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 229940100243 oleanolic acid Drugs 0.000 description 1
- 208000011906 peptic ulcer disease Diseases 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- NWMIYTWHUDFRPL-UHFFFAOYSA-N sapogenin Natural products COC(=O)C1(CO)C(O)CCC2(C)C1CCC3(C)C2CC=C4C5C(C)(O)C(C)CCC5(CCC34C)C(=O)O NWMIYTWHUDFRPL-UHFFFAOYSA-N 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 230000007863 steatosis Effects 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009747 swallowing Effects 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- KBPHJBAIARWVSC-XQIHNALSSA-N trans-lutein Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C(=CC(O)CC2(C)C)C KBPHJBAIARWVSC-XQIHNALSSA-N 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- FJHBOVDFOQMZRV-XQIHNALSSA-N xanthophyll Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CC(O)CC1(C)C)C=CC=C(/C)C=CC2C=C(C)C(O)CC2(C)C FJHBOVDFOQMZRV-XQIHNALSSA-N 0.000 description 1
- 235000008210 xanthophylls Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
- A23L2/395—Dry compositions in a particular shape or form
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/754—Evodia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
- A61K9/0007—Effervescent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Diabetes (AREA)
- Insects & Arthropods (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Emergency Medicine (AREA)
- Obesity (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses an effervescent tablet and a preparation method and application thereof. The effervescent tablet comprises an active ingredient and an auxiliary material, wherein the active ingredient comprises the following components in parts by mass: 15-30 parts of extract, 210-30 parts of extract, 310-20 parts of extract, 45-20 parts of extract and 515 parts of extract; the raw materials for extracting the extract 1 comprise: 30 or 35 parts of actinidia arguta, 20-40 parts of okra and 10-40 parts of Chinese yam; the raw materials for extracting the extract 2 comprise: 30 or 40 parts of mulberry, 20-50 parts of blackcurrant and 10-40 parts of blueberry; the raw materials for extracting the extract 3 comprise: 5 parts of codonopsis pilosula, 5-10 parts of ligusticum wallichii, 20-30 parts of dandelion, 20-30 parts of roasted malt, 5-10 parts of fructus evodiae and 10-30 parts of Chinese date; the raw material for extracting the extract 4 comprises the skin of black rice; the raw material for extracting the extract 5 comprises honey. The effervescent tablet provided by the invention is applied to preparing products with the following functions: improving and repairing gastric ulcer; at least one active function of antioxidation, antifatigue, antitumor and hypoglycemic; protecting liver.
Description
Technical Field
The invention relates to an effervescent tablet and a preparation method and application thereof, belonging to the field of health food processing and application.
Background
Gastric ulcer refers to ulcer occurring in the gastric horn, antrum, cardia, and hiatal hernia, and is one of peptic ulcers. Peptic ulcer is a common digestive tract disease, and may occur in esophagus, stomach or duodenum, and is generally referred to as gastric ulcer and duodenal ulcer because gastric ulcer and duodenal ulcer are most common. The traditional Chinese medicine composition belongs to common diseases which are easy to relapse, the factors for inducing ulcer are complex, helicobacter pylori infection is one of the main factors, and the medicines, diet and immunity can induce, so that the combined treatment of acid gastric inhibition, inflammation resistance and gastric membrane protection is mainly adopted at the present stage, the stable treatment effect is difficult to realize, and the good treatment effect can be obtained by adopting the traditional Chinese medicine to treat the chronic diseases, and the chronic diseases are not easy to relapse. In addition, the helicobacter pylori infection can induce gastric cancer, so that the traditional Chinese medicine compound function is exerted, and the reduction of malignant lesion probability can not be realized by the western medicine treatment at the present stage while the ulcer is treated.
The flavonoid compound has a 2-phenyl chromone (flavanone) structure, and has cardiovascular system activity, antibacterial and antiviral activity, antitumor activity, antioxidant free radical activity, blood pressure lowering, blood lipid reducing, anti-aging, organism immunity improving and the like.
Anthocyanins are compounds formed by combining anthocyanidins and sugars, are used as natural pigments, and are safe and nontoxic. Due to its unique functionality, it is used in eliminating free radicals in vivo, proliferating xanthophyll, resisting tumor, resisting cancer, resisting inflammation, inhibiting lipid peroxidation and platelet aggregation, preventing diabetes, reducing weight, protecting vision, etc., and has been used in food, health product, cosmetic, medicine, etc. Different types of anthocyanin have different biological activities, and different physiological and biochemical activities can be obtained by adopting different types of compound combinations.
The triterpene and saponin are composed of triterpene sapogenin and sugar or uronic acid, have effects of resisting inflammation, promoting blood circulation for removing blood stasis, lowering blood pressure, relieving pain, tranquilizing mind, resisting tumor, and resisting cancer, and are one of the active marker components of Chinese medicinal materials.
Polysaccharides (polysaccharides) are polymeric carbohydrate macromolecules consisting of glycosidically bonded sugar chains and at least more than 10 monosaccharides. Has biological activities of regulating immunity, resisting virus, resisting cancer, resisting inflammation, resisting oxidation, caring skin, and emulsifying etc.
The effervescent tablet has the advantages of convenient use, quick disintegration, convenient administration, quick action, high bioavailability, improved clinical curative effect, suitability for children, the elderly and patients with difficulty in swallowing pills, better taste, and greatly improved drug applicability.
Disclosure of Invention
The invention aims to provide an effervescent tablet and a preparation method and application thereof, and the invention adopts a flash extraction method, an enzymatic microwave method, an enzymatic ultrasonic wave method and other extraction methods according to different raw material states, and adopts macroporous resin, LH-20 and other purification methods to realize a novel effervescent agent with definite active ingredients, relatively high content of the ingredients and definite drug effect. The obtained preparation has the advantages of convenient use, rapid onset of drug action, high bioavailability, low burden on gastrointestinal tract, etc. Meanwhile, the active ingredients obtained by the formula have good effects of resisting ulcer, repairing mucosa and preventing canceration; in addition, through an acute toxicity test, the related products are nontoxic, can be taken for a long time, and have obvious health care functions of resisting oxidation, fatigue, tumor and the like, so the health care tea has good market prospect.
The invention provides an effervescent tablet which comprises active ingredients and auxiliary materials, wherein the active ingredients comprise the following components in parts by mass:
15-30 parts of extract, 210-30 parts of extract, 310-20 parts of extract, 45-20 parts of extract and 515 parts of extract;
the raw materials for extracting the extract 1 comprise the following components in parts by mass: 30 or 35 parts of actinidia arguta, 20-40 parts of okra and 10-40 parts of Chinese yam;
the raw materials for extracting the extract 2 comprise the following components in parts by mass: 30 or 40 parts of mulberry, 20-50 parts of blackcurrant and 10-40 parts of blueberry;
the raw materials for extracting the extract 3 comprise the following components in parts by mass: 5 parts of codonopsis pilosula, 5-10 parts of ligusticum wallichii, 20-30 parts of dandelion, 20-30 parts of roasted malt, 5-10 parts of fructus evodiae and 10-30 parts of Chinese date;
the raw material for extracting the extract 4 comprises the skin of black rice;
the raw material for extracting the extract 5 comprises honey.
In the effervescent tablet, the auxiliary materials comprise the following components in parts by mass:
10-20 parts of sodium bicarbonate, 5-15 parts of citric acid, 1-20 parts of xylitol, 0-10 parts of sorbitol, 1-3 parts of an adhesive, 1-5 parts of sodium alginate and 1-3 parts of a lubricant.
In the effervescent tablet, the auxiliary materials also comprise a proper amount of absolute ethyl alcohol and water according to the preparation requirement.
In the effervescent tablet, the adhesive is at least one of PEG2000, PEG4000, PEG6000 and PVP;
the lubricant is at least one of superfine silica gel powder, talcum powder and magnesium stearate.
In the effervescent tablet, the active ingredients of the effervescent tablet comprise the following components in parts by mass:
110-25 parts of extract, 210-25 parts of extract, 310-20 parts of extract, 410-20 parts of extract and 515 parts of extract;
the raw materials for extracting the extract 1 comprise the following components in parts by mass: 30 or 35 parts of actinidia arguta, 25-35 parts of okra and 30 parts of Chinese yam;
the raw materials for extracting the extract 2 comprise the following components in parts by mass: 30 or 40 parts of mulberry, 30-40 parts of blackcurrant and 30 parts of blueberry;
the raw materials for extracting the extract 3 comprise the following components in parts by mass: 5 parts of codonopsis pilosula, 5-10 parts of ligusticum wallichii, 20-30 parts of dandelion, 20-30 parts of roasted malt, 5-10 parts of fructus evodiae and 10-30 parts of Chinese date.
The active ingredients of the effervescent tablet comprise the following components in parts by mass:
115 parts of extract, 215 parts of extract, 315 parts of extract, 410 parts of extract, 515 parts of extract, 10 parts of sodium bicarbonate, 15 parts of citric acid, 2 parts of adhesive (PEG6000), 4 parts of xylitol, 3 parts of sodium alginate, 1 part of lubricant (aerosil), a proper amount of absolute ethyl alcohol and water;
wherein, the weight ratio of the extract 1: 35 parts of actinidia arguta, 35 parts of okra and 30 parts of Chinese yam;
and (2) extraction: 30 parts of mulberry, 40 parts of blackcurrant and 30 parts of blueberry;
and (3) extracting: 5 parts of codonopsis pilosula, 5 parts of ligusticum wallichii, 30 parts of dandelion, 30 parts of fried malt, 10 parts of fructus evodiae and 20 parts of Chinese date.
The active ingredients of the effervescent tablet comprise the following components in parts by mass:
115 parts of extract, 220 parts of extract, 310 parts of extract, 410 parts of extract, 515 parts of extract, 10 parts of sodium bicarbonate, 15 parts of citric acid, 2 parts of adhesive (PEG6000), 3 parts of xylitol, 3 parts of sodium alginate, 2 parts of lubricant, a proper amount of absolute ethyl alcohol and water;
wherein, the weight ratio of the extract 1: 30 parts of actinidia arguta, 40 parts of okra and 30 parts of Chinese yam;
and (2) extraction: 40 parts of mulberry, 30 parts of blackcurrant and 30 parts of blueberry;
and (3) extracting: 5 parts of codonopsis pilosula, 5 parts of ligusticum wallichii, 30 parts of dandelion, 20 parts of fried malt, 10 parts of fructus evodiae and 30 parts of Chinese date.
In the effervescent tablet, the extract 1 is extracted by adopting a flash extraction method;
extracting the extract 2 by adopting a flash extraction method and a mixed resin method;
extracting the extract 3 by using an enzymatic-microwave-ultrasonic extraction method;
extracting the extract 4 by using an enzymatic-ultrasonic and macroporous resin method;
the extract 5 is extracted by adopting a freeze-dried powder preparation method.
In the effervescent tablet, the method for extracting the extract 1 comprises the following steps: taking water as an extracting agent, crushing fresh actinidia arguta, okra and Chinese yam, adding cellulase and hemicellulase for treatment, and inactivating; then flash extraction is adopted to obtain an extracting solution; after the extracting solution is centrifugally treated, adding vitamin C and drying to obtain an extract 1;
the method for extracting the extract 2 comprises the following steps: mixing Mori fructus, blackcurrant, and fructus Myrtilli with ethanol-water as solvent, extracting by flash rapid extraction method, adding pectase, cellulase and hemicellulase, inactivating, extracting the obtained enzymolysis extractive solution by flash rapid extraction method, filtering to obtain extractive solution, and concentrating; purifying with AB-8 and LH-20 as mixed purification materials by mixed resin method to obtain extract 2 concentrated solution, and freeze drying to obtain extract 2;
the method for extracting the extract 3 comprises the following steps: pulverizing radix Codonopsis, rhizoma Ligustici Chuanxiong, herba Taraxaci, fructus Hordei Germinatus preparata, fructus evodiae, and fructus Jujubae, extracting with ethanol-water as extraction solvent, adding cellulase and hemicellulase, and performing enzymatic treatment; then carrying out microwave treatment; adding amylase into a microwave system, performing ultrasonic extraction, concentrating, and drying to obtain an extract 3;
the method for extracting the extract 4 comprises the following steps: crushing black rice, taking black rice peel, adding amylase, performing enzymatic treatment, and then performing microwave treatment; ultrasonic extracting for 1-3 times with hydrochloric acid water solution as solvent, concentrating the obtained extract at low temperature, pre-freezing, and freeze-drying; then eluting to obtain active ingredients, and freeze-drying to obtain extract 4;
the method for extracting the extract 5 comprises the following steps: adding anhydrous ethanol into Mel, adding sorbitol and xylitol, ultrasonic treating, centrifuging, filtering, concentrating the ethanol extract, and spray drying to obtain spray dried product; pre-freezing the non-alcohol-soluble part at-20 ℃, and freeze-drying to obtain freeze-dried powder; mixing the freeze-dried powder and the spray-dried product to obtain an extract 5.
In the effervescent tablet, the extract 1 is extracted according to the following steps: crushing the actinidia arguta, the okra and the Chinese yam, wherein the solid-liquid volume ratio of the total amount of the actinidia arguta, the okra and the Chinese yam to the water is 1: 1-6, the pH is 5-7, and cellulase (the enzyme activity is 50000U/g) with the mass percentage concentration of 1-2% and hemicellulase (the enzyme activity is 3000U/g) with the mass percentage concentration of 0.5-2% are added into every 100g of actinidia arguta, okra and Chinese yam for treatment for 1-2 hours; inactivating for 1min at 80-100 ℃; transferring the mixture into a flash extractor for extraction for 150-200 s, extracting at the voltage of 180-220v and the extraction temperature of 10-20 ℃, and obtaining an extracting solution; centrifuging the extracting solution at 3000-5000 r/min for 5-20 min, adding 0.5-2% by mass of vitamin C, and spray drying to obtain an extract 1;
the extraction of the extract 2 was carried out according to the following steps: mixing mulberries, blackcurrants and blueberries with 10-30% of ethanol-water by volume percentage, extracting for 50-100 s by adopting a flash type rapid extraction method according to the solid-liquid volume ratio of 1: 5-10, adding 0.5-1% of pectinase (the enzyme activity is 40000U/g), 1-2% of cellulase (the enzyme activity is 50000U/g) and 1-2% of hemicellulase (the enzyme activity is 3000U/g) by mass percentage per 100g of solid, carrying out enzymatic treatment for 1-2 h at the pH value of 4-7 and the temperature of 30-40 ℃, inactivating for 1min at the temperature of 80-100 ℃, extracting an enzymolysis extracting solution again by adopting a flash type rapid extraction method, filtering to obtain an extracting solution, and concentrating; the ratio of AB-8: purifying by a mixed resin method by using mixed purification materials LH-20: 0.2-5, performing gradient elution by using 10-90% ethanol-water by volume percentage to obtain an extract 2 concentrated solution, freeze-drying, crushing, and sieving by using a 80-mesh sieve to obtain an extract 2;
the extraction of the extract 3 was carried out according to the following steps: crushing codonopsis pilosula, ligusticum wallichii, dandelion, whole plants, roasted malt, fructus evodiae and Chinese date, taking 5-40% ethanol-water in percentage by volume as an extraction solvent, adding cellulase (the enzyme activity is 50000U/g) with the mass percentage concentration of 2-4% and hemicellulase (the enzyme activity is 3000U/g) with the mass percentage concentration of 1-2% into each 100g of solid at the solid-liquid volume ratio of 1: 10-20, the pH value of 5-7 and the temperature of 30-40 ℃, and carrying out enzymatic treatment for 2-4 hours; microwave treatment is carried out for 2-4 min under the microwave power of 10-30 KW; transferring the microwave system into an ultrasonic extractor, and extracting the functional additive at an ultrasonic extraction temperature of 50-80 ℃ for 30-60 min at an ultrasonic frequency of 20-30 kHz; recovering the ethanol solvent, concentrating to 1/8-1/4 of the original volume, and spray drying to obtain the extract 3;
the extraction of the extract 4 was carried out according to the following steps: crushing black rice, taking black rice peel, adding alpha-amylase (the enzyme activity is 10000U/g) with the mass percentage concentration of 1-5% into every 100g of solid, performing enzymatic treatment for 2-4 h at the temperature of 50-60 ℃, wherein the microwave power is 10-20 KW, and performing microwave treatment for 1-2 min; taking a hydrochloric acid aqueous solution with the volume percentage content of 0.1-1% as a solvent, carrying out ultrasonic treatment at the temperature of 30-50 ℃, carrying out ultrasonic treatment at the power of 200-800W for 60-120 min, extracting for 2 times, concentrating the obtained extracting solution at low temperature to the original volume of 1/5-1/10, pre-freezing at the temperature of-20 ℃, and carrying out freeze drying; and (2) taking the ratio of D101: AB-8: performing gradient elution on the D201-1: 1: 1-5 ethanol-water system with the volume percentage of 10-90% to obtain an active ingredient, and performing freeze drying to obtain the extract 4;
taking the extract 5 according to the following steps: adding absolute ethyl alcohol into honey to enable the volume content of the ethyl alcohol to exceed 70-90%, adding 5-10% of sorbitol according to 100 g: and (2) carrying out ultrasonic treatment on xylitol at the ratio of 1:1 for 5-10 min, carrying out centrifugal filtration, concentrating and spray-drying an alcohol extraction part for later use, carrying out pre-freezing on a non-alcohol-soluble part at the temperature of-20 ℃, carrying out freeze-drying, and mixing the freeze-dried powder and a spray-dried product to obtain the extract 5.
The invention also provides a preparation method of the active ingredients of the effervescent tablet, which comprises the following steps: extracting the extract 1 by adopting a flash extraction method, extracting the extract 2 by adopting a flash extraction method and a mixed resin method, extracting the extract 3 by adopting an enzymatic-microwave-ultrasonic extraction method, extracting the extract 4 by adopting an enzymatic-ultrasonic and macroporous resin method, and extracting the extract 5 by adopting a freeze-dried powder preparation method to obtain the active ingredient of the effervescent tablet.
In the invention, the specific preparation method of the active ingredients of the effervescent tablet is the same as that of the effervescent tablet in the invention.
The invention also provides a preparation method of the effervescent tablet, which comprises the following steps:
mixing the extract 1, the extract 3, the extract 4 and the extract 5 with the citric acid and the xylitol, and granulating, drying and grading to obtain an effervescent acid phase; mixing the extract 2 with the sodium bicarbonate and the sodium alginate, and performing dry granulation and granule finishing to obtain an effervescent base phase; and mixing the effervescent acid phase and the effervescent alkali phase, adding the lubricant, mixing, tabletting and packaging to obtain the effervescent tablet.
In the invention, the preparation method of the effervescent tablet also comprises a step of adding sorbitol during freeze-drying so as to improve the efficiency.
In the preparation method of the effervescent tablet, a proper amount of absolute ethyl alcohol and water are also added during preparation.
The effervescent tablet disclosed by the invention is applied to the preparation of a product with any one of the following functions 1) -3):
1) improving and repairing gastric ulcer;
2) at least one active function of antioxidation, antifatigue, antitumor and hypoglycemic;
3) protecting liver.
The invention has the following advantages:
the invention adopts the extraction methods of a flash extraction method, enzymatic microwaves, enzymatic ultrasonic waves and the like according to different raw material states, and adopts the purification methods of macroporous resin, LH-20 and the like to realize the novel effervescent agent with definite active ingredients, relatively high content of the ingredients and definite drug effect. The obtained preparation has the advantages of convenient use, rapid onset of drug action, high bioavailability, low burden on gastrointestinal tract, etc. Meanwhile, the active ingredients obtained by the formula have good effects of resisting ulcer, repairing mucosa and preventing canceration; in addition, through an acute toxicity test, the related products are non-toxic, can be taken for a long time, and have remarkable health care functions of oxidation resistance, fatigue resistance, tumor resistance and the like, so the health care tea has good market prospect.
Drawings
FIG. 1 is a graph showing the effect of the present invention on the improvement and repair of gastric ulcer.
FIG. 2 is a pathological diagram of a typical ethanol ulcer.
FIG. 3 is a pathological diagram of a typical histamine phosphate group.
FIG. 4 shows the death of the mice in the groups of the present invention in the experiment for improving and repairing gastric ulcer.
FIG. 5 is a graph showing the effect of the present invention on the scavenging ability of hydroxyl radicals.
FIG. 6 is a graph showing the effect of the present invention on DPPH radical scavenging ability.
FIG. 7 is a graph showing the effect of the present invention on antitumor activity.
FIG. 8 is a graph showing the results of the effect of the present invention on the body weight of mice.
FIG. 9 is a graph showing the results of the effect of the present invention on blood glucose levels in mice.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The method of detection of the active ingredients in the following examples: determining anthocyanin by a pH differential method; determining total flavonoids by an aluminum nitrate-sodium nitrite colorimetric method; measuring total polysaccharide by a sulfuric acid-phenol method; triterpene and saponin were determined by vanillin-perchloric acid chromogenic method.
The following examples use the main apparatus: FY130 pulverizer, tianjin tesla; JHBE-100A flash extractor, Henan Zhi Jing Biotech, Inc.; DP-SY-360 ultrasonic extractor, Oudepeng science and technology, Beijing Asia; NJL07-3 model microwave oven for experiment, Nanjing Jie microwave Equipment Co., Ltd; TGL-21M full-automatic high-speed refrigerated centrifuge, Shanghai Luxiang apparatus centrifuge instruments Ltd; t6 ultraviolet visible spectrophotometer, Beijing spectral analysis; model PB-10 acidimeters, ME203E electronic balance, Saedodes scientific instruments (Beijing); JIUPIN-150 spray dryer, Wuxi Jiping instruments ltd; synergy4 multifunctional microplate reader, american nubuck et al.
In the following examples, anthocyanin assay:
the anthocyanin is measured by adopting a pH differential method, and the calculation formula is as follows:
the formula is C (mg/g) ═ A0-A1 x V x n x M/(epsilon x M)
Wherein A0 and A1 are the absorbance values of anthocyanin at lambda max at pH1.0 and pH4.5, respectively
V-Total volume of extract (mL)
n-dilution multiple
Relative molecular mass of M-cornflower-3-glucoside (449.4)
Extinction coefficient of EE-cornflower-3-glucoside (29600)
m-sample mass (g)
② measuring the total flavone:
the sodium nitrite-aluminum nitrate method is adopted. Rutin is used as standard substance, and total flavone is determined at 500 nm.
Solid preparation determination: extracting with methanol as solvent, recovering solvent after ultrasonic extraction, concentrating, drying, dissolving with small amount of methanol, adding 70% methanol to a volume of 100mL, and transferring 1mL mother liquor to 25mL colorimetric tube to determine content according to the above method.
③ measuring total polysaccharide:
glucose is used as a standard substance, and a sulfuric acid-phenol color development method is adopted to determine total polysaccharide at 490 nm.
Solid preparation determination: ultrasonic treating with hot water as solvent, adding ethanol to reach concentration of over 80%, standing for 10 hr, centrifuging, decolorizing with acetone, precipitating with ethanol again, centrifuging, and drying. Taking a proper amount of pure water as a solvent, fixing the volume to a 100mL volumetric flask, and transferring 1mL of mother liquor to a 25mL colorimetric tube to measure the content according to the method.
Determination of triterpene and saponin:
taking oleanolic acid as a standard substance, and measuring an absorbance A at a wavelength of 545nm by adopting a 5% vanillin-perchloric acid color development method.
Solid preparation determination: ultrasonic extraction is carried out by taking 70% ethanol as solvent, chloroform and n-butanol are sequentially carried out for extraction after concentration, and 0.5-2mL of extract liquid is precisely transferred to a 25mL colorimetric tube for content determination according to the method.
Examples 1,
(1) Active substance extraction:
extracting an extract 1: using water as an extracting agent, adding 1% of vitamin C, and then crushing 35 parts of actinidia arguta, 35 parts of okra and 30 parts of Chinese yam, wherein the solid-liquid ratio (volume ratio) is 1: 6. the pH value is 5.5, cellulase with the mass percentage concentration of 1% (the enzyme activity is 50000U/g) and 2% hemicellulase (the enzyme activity is 3000U/g) are added into every 100g of solid for treatment for 2 hours, and inactivation is carried out for 1min at the temperature of 100 ℃; transferring the sample into a flash extractor, wherein the extraction time is 150s, the extraction voltage is 180-: extracting at 20 deg.C, centrifuging at 4000r/min for 15min, and spray drying to obtain extract 1.
And (3) extracting an extract 2: 30 parts of mulberry, 40 parts of blackcurrant and 30 parts of blueberry. Extracting 100s by using 10% ethanol-water as a solvent and a flash type rapid extraction method with a solid-to-liquid ratio of 1:10, adding 2% by mass of pectinase (the enzyme activity is 40000U/g), 1% by mass of cellulase (the enzyme activity is 50000U/g) and 0.5% by mass of hemicellulase (the enzyme activity is 3000U/g) into each 100g of solid, controlling the pH to be 6, carrying out enzymolysis at 30 ℃ for 2h, inactivating at 100 ℃ for 1min, carrying out flash type rapid extraction on the enzymolysis extracting solution for 200s again, filtering to obtain an extracting solution, recovering the ethanol solvent, and concentrating to the original volume of 1/5; mixing AB-8 and LH-20 (2:1) as purifying materials, eluting with 30% ethanol water by mixed resin method to obtain extract 2 concentrated solution, freeze drying, pulverizing, and sieving with 80 mesh sieve to obtain extract 2.
And (3) extracting: crushing 5 parts of codonopsis pilosula, 5 parts of ligusticum wallichii, 30 parts of dandelion, 30 parts of roasted malt, 10 parts of fructus evodiae and 20 parts of Chinese date, taking 30% ethanol-water as an extraction solvent, adding 2% cellulase (the enzyme activity is 50000U/g) and 1% hemicellulase (the enzyme activity is 3000U/g) into each 100g of solid, wherein the solid-liquid ratio (the volume ratio) is 1:15, the pH value is 6, and the temperature is 30 ℃, and carrying out enzymatic treatment for 2 hours; microwave treatment with microwave power of 30KW for 2 min; and transferring the microwave system into an ultrasonic extractor, and extracting the functional additive at an ultrasonic frequency of 30kHz and an ultrasonic extraction temperature of 50 ℃ for 60 min. Recovering ethanol solvent, concentrating to original volume of 1/8, and spray drying to obtain extract 3.
And (4) extracting: crushing black rice, sieving with 14-20 mesh sieve, collecting black rice peel, adding 2% amylase alpha-amylase (enzyme activity is 10000U/g) per 100g solid, performing enzymatic treatment at 50 deg.C for 2 hr, and performing microwave treatment with microwave power of 20KW for 2 min; extracting with 0.1% hydrochloric acid water solution as solvent at 30 deg.C under 400W for 60min for 2 times, concentrating the extractive solution at low temperature to original volume of 1/10, prefreezing at-20 deg.C, and freeze drying; with D101: AB-8: and (3) carrying out gradient elution on the D201-1: 1, 60% ethanol-water system to obtain an active ingredient, and freeze-drying to obtain an extract 4 for later use.
And (3) extracting an extract 5: adding anhydrous ethanol into honey to make ethanol content exceed 90%, adding 5% sorbitol according to 100 g: treating xylitol at a ratio of 1:1 with ultrasound for 10min, centrifuging, filtering, concentrating the ethanol extract, spray drying, pre-freezing at-20 deg.C, freeze drying, and mixing the lyophilized powder and spray dried product to obtain extract 5.
(2) Preparation: the effervescent formula is characterized in that: 115 parts of extract, 215 parts of extract, 315 parts of extract, 410 parts of extract, 515 parts of extract, 10 parts of sodium bicarbonate, 15 parts of citric acid, 2 parts of adhesive (PEG6000), 4 parts of xylitol, 3 parts of sodium alginate, 1 part of lubricant (aerosil), a proper amount of absolute ethyl alcohol and water.
Mixing the extracts 1, 3, 4 and 5 with citric acid and xylitol, adding adhesive, granulating, drying at 50 deg.C, and sieving with 20 mesh sieve to obtain effervescent acid phase; mixing the extract 2 with sodium bicarbonate and sodium alginate, granulating by dry method, grading to obtain effervescent base phase, mixing the two phases, and packaging to obtain effervescent.
(3) And (4) checking: determination of anthocyanin by pH differential method: total anthocyanin 32.34%; according to the previous flavone detection method, the standard curve Y is 0.581x +0.015, R 2 0.9993, total flavone content 12.38%. According to the previous polysaccharide determination method, the standard curve Y is 0.017x +0.025, R 2 0.9996, total polysaccharide content 32.4%; its standard curve Y ═ 0.033x +0.037, R 2 Total triterpenes and saponins were 6.12%, 0.9905%.
Examples 2,
(1) Active substance extraction:
extracting an extract 1: taking water as an extracting agent, adding 1% of vitamin C, and then crushing 30 parts of actinidia arguta, 40 parts of okra and 30 parts of Chinese yam in a solid-liquid ratio (volume ratio) of 1: 5. adding cellulase (with enzyme activity of 50000U/g) with a mass percent concentration of 1% and hemicellulase (with enzyme activity of 3000U/g) with a mass percent concentration of 2% into each 100g of solid at a pH of 6, treating for 2h, and inactivating for 1min at 100 ℃; transferring the sample into a flash extractor, wherein the extraction time is 100s, the extraction voltage is 180-: extracting at 20 deg.C, centrifuging at 4000r/min for 10min, and spray drying to obtain extract 1.
And (3) extracting an extract 2: 40 parts of mulberry, 30 parts of blackcurrant and 30 parts of blueberry. Extracting 100s by using 20% ethanol-water as a solvent and a flash type rapid extraction method with a solid-to-liquid ratio of 1:10, adding 1% by mass of pectinase (the enzyme activity is 40000U/g), 1% by mass of cellulase (the enzyme activity is 50000U/g) and 1% by mass of hemicellulase (the enzyme activity is 3000U/g) into each 100g of solid, controlling the pH to be 6, carrying out enzymolysis at 30 ℃ for 2h, inactivating at 100 ℃ for 1min, carrying out flash type rapid extraction on the enzymolysis extracting solution for 200s again, filtering to obtain an extracting solution, recovering an ethanol solvent, and concentrating to the original volume of 1/5; purifying with AB-8 and LH-20 as mixed (1:1) purification material by mixed resin method, eluting with 60% ethanol-water to obtain extract 2 concentrate, freeze drying, pulverizing, and sieving with 80 mesh sieve to obtain extract 2.
And (3) extracting: crushing 5 parts of codonopsis pilosula, 5 parts of ligusticum wallichii, 30 parts of dandelion (whole grass), 20 parts of roasted malt, 10 parts of fructus evodiae and 30 parts of Chinese dates, taking 30% ethanol-water as an extraction solvent, adding 1% cellulase (the enzyme activity is 50000U/g) and 1% hemicellulase (the enzyme activity is 3000U/g) in mass percentage concentration into 100g of solid, and performing enzymatic treatment for 2 hours, wherein the solid-liquid ratio (the volume ratio) is 1:20, the pH value is 6, and the temperature is 30 ℃; microwave treatment with 30KW power for 4 min; and transferring the microwave system into an ultrasonic extractor, and extracting the functional additive at the ultrasonic frequency of 30kHz and the ultrasonic extraction temperature of 40 ℃ for 60 min. Recovering ethanol solvent, concentrating to original volume of 1/5, and spray drying to obtain extract 3.
And (4) extracting: crushing black rice, sieving with a 20-mesh sieve, taking black rice peel, adding alpha-amylase (with the enzyme activity of 10000U/g) with the mass percentage concentration of 4% into every 100g of solid, performing enzymatic treatment for 2 hours at the temperature of 50 ℃, performing microwave treatment for 2 minutes at the microwave power of 20 KW; extracting with 0.5% hydrochloric acid water solution as solvent at 30 deg.C under 600W for 60min for 2 times, concentrating the extractive solution at low temperature to original volume of 1/8, prefreezing at-20 deg.C, and freeze drying; and (2) taking the ratio of D101: AB-8: and (3) carrying out gradient elution on the D201-1: 1:2 by using a 60% ethanol-water system to obtain an active ingredient, and carrying out freeze drying to obtain an extract 4 for later use.
And (3) extracting an extract 5: adding anhydrous ethanol into honey to make ethanol content exceed 90%, adding 6% sorbitol according to 100 g: treating xylitol at a ratio of 1:1 with ultrasound for 10min, centrifuging, filtering, concentrating the ethanol extract, spray drying, pre-freezing at-20 deg.C, freeze drying, and mixing the lyophilized powder and spray dried product to obtain extract 5.
(2) Preparation: the effervescent formula is characterized in that: 115 parts of extract, 220 parts of extract, 310 parts of extract, 410 parts of extract, 515 parts of extract, 10 parts of sodium bicarbonate, 15 parts of citric acid, 2 parts of adhesive (PEG6000), 3 parts of xylitol, 3 parts of sodium alginate, 2 parts of lubricant, a proper amount of absolute ethyl alcohol and water.
Mixing the extracts 1, 3, 4 and 5 with citric acid and xylitol, adding binder, granulating, drying at 50 deg.C, and sieving with 20 mesh sieve to obtain effervescent acid phase; mixing the extract 2 with sodium bicarbonate and sodium alginate, granulating by dry method, grading to obtain effervescent base phase, mixing the base phase and base phase, adding lubricant (stearic acid), tabletting, and packaging to obtain effervescent tablet.
(3) And (4) checking: and (3) determining anthocyanin by adopting a pH differential method: total anthocyanin is 31.05%; according to the detection method of the flavone, the standard curve Y is 0.581x +0.015, R 2 0.9993, total flavone content 13.4%. According to the previous polysaccharide determination method, the standard curve Y is 0.017x +0.025, R 2 0.9996, total polysaccharide content 31.6%; its standard curve Y ═ 0.033x +0.037, R 2 Total triterpenes and saponins were 6.02%, 0.9905%.
Examples 3,
First, acute toxicity test
Acute toxicity test: 30 rats are randomly divided into 10 rats in a blank group, a control group and an experimental group, and water is not forbidden and fasting is carried out for 6 hours before the experiment. The experimental group administered effervescent agent (crushed before tabletting through 100-200 mesh, sterilized distilled water, suspension obtained by high-speed homogenizer treatment) suspension liquid into stomach in 1d for 3 times, wherein each time is 0.25g/mL, the maximum stomach capacity is 4m L/100g, the interval between two times of stomach administration is 6h, and after stomach administration, fasting and water prohibition are carried out for 4 h; the control group is given preparation auxiliary material suspension liquid in the same way as the experimental group; the blank group was subjected to gavage with the same amount of physiological saline as the experimental group. After gavage, three groups of rats were continuously observed for hair, respiration, food intake, feces, oronasal secretion, mental state and body mass change for 1 time each in the morning and afternoon every day for 14 days; and the time to onset, recovery and death was recorded. And (3) measuring the body weight: the recording was started from the 1 st gavage and the measurements were taken 1, 5, 10, 14d after gavage. The next day after the experiment, the rats were sacrificed by dislocation, and the external and internal organs of the rats were dissected and visually observed, and histopathological examination was performed after abnormality was found.
The maximum dose in the experimental group was 0.25g/mL × 40mL/kg (maximum gastric volume) × 3 times to 30g/kg, which is 180 times the maximum daily recommended daily dose for adults (10g/60 kg). After the test group was gavaged at the maximum administration concentration and the maximum gastric volume, the food intake and activity decreased significantly, but recovered to normal from day 2. Continuously observing for 7d, wherein the experimental group has black excrement on the next day and returns to normal after 5 days; the experimental group, the control group and the blank group have no obvious abnormality in hair, respiration, food intake, oral and nasal secretion, mental state and physical quality, and have no death or other abnormal states.
As shown in Table 1, the body mass of the experimental group given 1, 5, 10 and 14d was (149.52 + -1.48), (187.32 + -2.04), (208.84 + -1.73) and (214.89 + -1.98) g, the control group was (151.27 + -1.58), (185.12 + -2.08), (209.32 + -2.18) and (218.16 + -2.54) g, and the blank group was (150.16 + -1.28), (189.24 + -2.84), (208.64 + -2.86) and (216.92 + -3.28) g. The weight comparison of the three groups at each time period was not statistically different (P > 0.05).
TABLE 1 quality Change in groups of rats
Note: (P > 0.05)
The above results show that the effervescent tablet of the present invention has good safety.
And II, improving and repairing gastric ulcer experiment:
taking male Kunming mice as experimental animals, taking a phosphoric acid histamine induction model, a water immersion restraint induction model and an ethanol induction model as experimental groups, carrying out a molding experiment, performing intragastric administration on extract suspension after molding, taking a tissue strip of 0.5 multiplied by 1.0cm close to the front wall of a pyloric gastric body of a antrum of the stomach to prepare a pathological section by detecting the preliminary judgment effects of weight, food intake, water intake, observation behavior and the like, considering pathological changes and a normal stomach wall in the junction of the pathological changes, and carrying out conventional HE dyeing and observation under a light microscope.
Main instrument
A mouse fixer; JV22-DST-671 model animal electronic scale, Beijing, Zhongxi, Ministry of science and technology; NV2L stereomicroscope, shanghai precision instruments ltd; MP-501A model constant temperature water bath, light mirror (microscope image acquisition system (OLYMPUS, BX 41)), and the like.
The molding method comprises the following steps:
50 mice were randomly divided into 4 groups, i.e., 10 blank control groups (normal control groups), 20 histamine phosphate groups, and 20 ethanol method groups, respectively. The day of molding was taken as day 1. The other 3 groups except the normal control group were fasted at 8:00 am on the first 1 day without water deprivation until 8:00 am on the first 1 day. The 1 st morning, 8:00, groups were chronologically ordered and molding was started in sequence. The method comprises the following specific steps:
A. blank (normal) control group: normal drinking and ingestion.
B. Phosphoric acid histamine group: and 8:00 in the morning, and performing intragastric administration. Concentration: 8 mg/ml; dosage: 0.8mg/10 g.
C. And (2) ethanol group: gavage was performed at 9:00 am. Dosage: 0.1ml/10g of absolute ethyl alcohol.
On the day 17:00, 2 patients in each group were dissected and observed for gastric molding; on the day 19:00, normal drinking water intake is recovered, the mice are raised conventionally, the performance of the mice is observed, and the mice in each group are randomly divided into 2 groups, wherein one group is an administration group, and the other group is treated by normal saline; the day 20:00 is treated by the first administration, the blank group is given with normal saline, the dose is 2mg/10g, the administration frequency is 3 times/24 h, the blank group is continuously fed for 15 days to be killed, and relevant results are detected.
The mice with ethanol and histamine phosphate in different states are relatively poor after the mice are subjected to model building, and the mice are observed to have gastric mucosa injuries in different degrees through deplaning, wherein after ethanol stimulation, gastric tissue edema has an increasing trend compared with a blank group control group in terms of weight, but the difference has no statistical significance. After 1 day after administration, the state of the mice with ethanol group and histamine phosphate is recovered, and the state of the water immersion restraint group is not obviously different from that of the control group.
The result of the repair effect of the invention on the gastric mucosa injury is shown in figure 1, and the pathological graphs of a typical ethanol ulcer and a typical histamine phosphate group in a comparison graph 2-3 in figure 1 can be obviously seen, which indicates that the modeling is successful in the experiment; compared with a control group, the experimental result shows that the ulcer surface is obviously formed after the ethanol and phosphoric acid amine group model building, the treatment is obviously improved after 15 days, in addition, the mouse death phenomenon is generated in the ethanol model building group (untreated group) and the phosphoric acid amine model building group (untreated group), the result is shown in figure 4, the survival mouse of the obviously administered group is obviously different from that of the untreated group, and the results before and after the treatment show that the gastric ulcer can be obviously improved and repaired.
Thirdly, the research result of the alcoholic liver injury model is as follows:
the effect of the extract mixture on the liver was studied using a rat alcoholic liver injury model: the liver indexes, TC, ALT and AST are detected by a full-automatic biochemical analyzer, MDA is detected by a TBA kit, SOD is detected by a xanthine oxidase kit method, GSH-PX is detected by a DTNB spectrophotometry method, and TG is detected by a KOH saponification-periodic acid oxidation kit.
The experimental process is as follows: the normal rats were fed with the feed for one week. 10 blank groups are divided, and 40 molding groups are manufactured. Feeding the blank group with common feed, performing intragastric administration with 10 mL/(kg. d) of pure water, and freely drinking the pure water; the model rats were randomly divided into a model group, a large dose group, a medium dose group and a small dose group. Each group had 10. The molding method is that the stomach is drenched by 50 percent ethanol 10mL/(kg d) in 2 times, and the timing is 9: 00: 00 and 21: 00, freely drinking 5% ethanol beverage (prepared by pure water), and feeding with common feed. The model group is perfused with pure water of 10 mL/(kg.d) during the molding; the large, medium and small dose groups are respectively perfused with suspension of 25 g/(kg.d), 15 g/(kg.d) and 5 g/(kg.d) during molding; the gavage time was fixed at 14 pm per day: 00. each group was sacrificed after weighing at the end of 12 weeks, blood was taken from the abdominal veins, fasting was performed 12 hours before blood was taken, and 5% ethanol beverage was freely drunk. The sacrificed rat is left to take liver tissue and weigh, observe the appearance, color, texture and section condition, take a small amount of liver middle tissue to prepare 0.85% sodium chloride homogenate, centrifuge and take supernatant fluid, and store in a refrigerator at minus 20 ℃ for standby.
② typical liver function index below, as shown in Table 1
TABLE 2 changes in blood lipid and liver function of rats in each group
TABLE 3 liver tissue SOD, MDA, GSHpx changes in rats of each group
Table 4 weight change of rats in each group
The body weight of the model group rats is obviously reduced compared with that of the blank group rats (P <0.01), and the body weight of the rats in the administration dose group is not obviously different from that of the model group. Model group rats had a significant increase in liver index compared to the blank group (P < 0.01). The liver index of the administration group is significantly reduced compared with that of the model group (P < 0.05). The rats in the model group have hepatic tissue steatosis and inflammatory infiltration, and the ALT, AST, TG and TC activities of the serum are obviously increased compared with those in the blank group. And the SOD and GSH-Px activity in the liver tissue is obviously reduced. The MDA content is obviously increased. The dosage of the administration group is improved in different degrees, so that the liver protection effect is achieved.
Fourth, the research results of antioxidation, antifatigue, antitumor, hypoglycemic are:
oxidation resistance
The hydroxyl radical scavenging capacity is measured by adopting phenanthroline-FeSO 4 -H 2 O 2 The hydroxyl radical scavenging capacity of the system research is calculated according to the following formula:
clearance (%) [ (Q1-Q2) ]/[ Q3-Q2] × 100
In the formula: q: sample + phenanthroline-FeSO 4 -H 2 O 2 Absorbance of the system solution; q: h 2 O 2 Absorbance of the solution + blank sample system solution; q3 without H 2 O 2 And absorbance of the system solution of the sample.
The DPPH free radical scavenging ability is measured by reacting at 522nm in the dark for 30min, and the scavenging ability is measured by the following calculation formula:
clearance (%) [1- (D1-D2)/D0] X100
In the formula: D02.5mLDPPH +1.5mL of absolute ethanol; d1, 2.5mL LDPPH +1.5mL of sample to be detected; d2: 2.5m absolute ethyl alcohol +1.5mL of sample to be tested.
The results are shown in fig. 5 and 6, which demonstrate that the effervescent tablet of the present invention has a function of scavenging free radicals.
② antifatigue
Anti-fatigue study of active ingredients of effervescent agent (effervescent tablet): the anti-fatigue activity was studied using a mouse (Kunming) weight model with swimming time and the content of lactic acid in whole blood as indices. The results are as follows:
(1) the effect on swimming time of Kunming mice is shown in Table 5.
TABLE 5 swimming test results
(2) The effect on blood lactate levels in Kunming mice is shown in Table 6
TABLE 6 Effect on blood lactate levels in mice
③ anti-tumor
The extract was subjected to antitumor study using 2 kinds of gastric cancer cells (MGC-803, SGC-7901) as target cells by MTT method. The results are shown in fig. 7, which shows that the effervescent tablet of the present invention has certain antitumor activity.
Fourthly, reducing blood sugar
(1) Effect on weight changes in mice
The average body weight of the mice at the basic level is 27.9g, and after one week of adaptation, a type 2 diabetes mouse model is induced and established in a mode of intraperitoneal injection of STZ. The mean value of the body weight after film formation was 34.2g, the body weight of the mice in the model group decreased to 26.6g after 8 weeks of continuous gavage, while the body weight of the mice in the normal group continued to increase from 33.3g after modeling to 42.5g, the body weight of the mice with type 2 diabetes decreased significantly at week 2, slightly at week 3 after administration, and the body weight of the mice in the model group continued to decrease even after 8 weeks of administration, as shown in table 7 and fig. 8.
TABLE 7 Effect on mouse body weight (g)
(2) Effect on the Change in blood glucose concentration in mice
After the model of the type 2 diabetes mouse is successfully established, the mouse is subjected to continuous 8-week gavage administration, and the fasting blood glucose concentration is measured once a week from the first day of the gavage administration. The mean value of the blood sugar concentration of the mice at the basic level is 6.28mmol/L, after one week of adaptation, the STZ is injected in the abdominal cavity to induce a type 2 diabetes mouse model, the mean value of the blood sugar concentration after film forming is 25.15mmol/L, after 8 weeks of continuous intragastric gavage administration, the blood sugar concentration of the mice in the model group is 27.92mmol/L, and after intragastric gavage administration, the blood sugar concentration of the mice in each administration group and the blood sugar concentration of the mice before intragastric gavage administration are obviously reduced, as shown in Table 8.
TABLE 8 Effect on mouse blood glucose levels (mmol/L)
Claims (7)
1. An effervescent tablet comprises an active ingredient and an auxiliary material, wherein the active ingredient comprises the following components in parts by mass:
15-30 parts of extract, 210-30 parts of extract, 310-20 parts of extract, 45-20 parts of extract and 515 parts of extract;
the raw materials for extracting the extract 1 comprise the following components in parts by mass: 30 or 35 parts of actinidia arguta, 20-40 parts of okra and 10-40 parts of Chinese yam;
the raw materials for extracting the extract 2 comprise the following components in parts by mass: 30 or 40 parts of mulberry, 20-50 parts of blackcurrant and 10-40 parts of blueberry;
the raw materials for extracting the extract 3 comprise the following components in parts by mass: 5 parts of codonopsis pilosula, 5-10 parts of ligusticum wallichii, 20-30 parts of dandelion, 20-30 parts of roasted malt, 5-10 parts of fructus evodiae and 10-30 parts of Chinese date;
the raw material for extracting the extract 4 is the skin of the black rice;
the raw material for extracting the extract 5 is honey;
the method for extracting the extract 1 comprises the following steps: taking water as an extracting agent, crushing fresh actinidia arguta, okra and Chinese yam, adding cellulase and hemicellulase for treatment, and inactivating; then flash extraction is adopted to obtain an extracting solution; after the extracting solution is centrifugally treated, adding vitamin C and drying to obtain an extract 1;
the method for extracting the extract 2 comprises the following steps: mixing mulberries, blackcurrants and blueberries with a solvent of ethanol-water, extracting by adopting a flash type rapid extraction method, adding pectinase, cellulase and hemicellulase for treatment, inactivating, extracting the obtained enzymolysis extracting solution by adopting the flash type rapid extraction method again, filtering to obtain an extracting solution, and concentrating; purifying with AB-8 and LH-20 as mixed purification materials by mixed resin method to obtain extract 2 concentrated solution, and freeze drying to obtain extract 2;
the method for extracting the extract 3 comprises the following steps: pulverizing radix Codonopsis, rhizoma Ligustici Chuanxiong, herba Taraxaci, fructus Hordei Germinatus preparata, fructus evodiae, and fructus Jujubae, extracting with ethanol-water as extraction solvent, adding cellulase and hemicellulase, and performing enzymatic treatment; then carrying out microwave treatment; performing ultrasonic extraction on a microwave system, concentrating and drying to obtain an extract 3;
the method for extracting the extract 4 comprises the following steps: crushing black rice, taking black rice peel, adding amylase, performing enzymatic treatment, and then performing microwave treatment; ultrasonic extracting for 1-3 times with hydrochloric acid water solution as solvent, concentrating the obtained extract at low temperature, pre-freezing, and freeze-drying; then eluting to obtain active ingredients, and freeze-drying to obtain extract 4;
the method for extracting the extract 5 comprises the following steps: adding anhydrous ethanol into Mel, adding sorbitol and xylitol, ultrasonic treating, centrifuging, filtering, concentrating the ethanol extract, and spray drying to obtain spray dried product; pre-freezing the non-alcohol-soluble part at-20 ℃, and freeze-drying to obtain freeze-dried powder; mixing the freeze-dried powder and the spray-dried product to obtain an extract 5.
2. An effervescent tablet according to claim 1, wherein: the auxiliary materials comprise the following components in parts by mass:
10-20 parts of sodium bicarbonate, 5-15 parts of citric acid, 1-20 parts of xylitol, 0-10 parts of sorbitol, 1-3 parts of an adhesive, 1-5 parts of sodium alginate and 1-3 parts of a lubricant.
3. An effervescent tablet according to claim 2, wherein: the adhesive is at least one of PEG2000, PEG4000, PEG6000 and PVP;
the lubricant is at least one of superfine silica gel powder, talcum powder and magnesium stearate.
4. An effervescent tablet according to any one of claims 1 to 3, wherein: the effervescent tablet comprises the following active ingredients in parts by mass:
110-25 parts of extract, 210-25 parts of extract, 310-20 parts of extract, 410-20 parts of extract and 515 parts of extract;
the raw materials for extracting the extract 1 comprise the following components in parts by mass: 30 or 35 parts of actinidia arguta, 25-35 parts of okra and 30 parts of Chinese yam;
the raw materials for extracting the extract 2 comprise the following components in parts by mass: 30 or 40 parts of mulberry, 30-40 parts of blackcurrant and 30 parts of blueberry;
the raw materials for extracting the extract 3 comprise the following components in parts by mass: 5 parts of codonopsis pilosula, 5-10 parts of ligusticum wallichii, 20-30 parts of dandelion, 20-30 parts of roasted malt, 5-10 parts of fructus evodiae and 10-30 parts of Chinese date.
5. An effervescent tablet according to claim 1, wherein: the extraction of the extract 1 was carried out according to the following steps: crushing the actinidia arguta, the okra and the Chinese yam, wherein the solid-liquid volume ratio of the total amount of the actinidia arguta, the okra and the Chinese yam to the water is 1: 1-6, the pH is 5-7, and cellulase with the mass percentage concentration of 1-2% and hemicellulase with the mass percentage concentration of 0.5-2% are added to each 100g of solid for treatment for 1-2 hours; inactivating for 1min at 80-100 ℃; transferring the mixture into a flash extractor for extraction for 150-200 s, extracting at the voltage of 180-220v and the extraction temperature of 10-20 ℃, and obtaining an extracting solution; centrifuging the extracting solution at 3000-5000 r/min for 5-20 min, adding 0.5-2% by mass of vitamin C, and spray drying to obtain an extract 1;
the extraction of the extract 2 was carried out according to the following steps: mixing mulberries, blackcurrants and blueberries with 10-30% of ethanol-water by volume percentage, extracting for 50-100 s by adopting a flash type rapid extraction method according to the solid-liquid volume ratio of 1: 5-10, adding pectinase with the mass percentage concentration of 0.5-1%, cellulase with the mass percentage concentration of 1-2% and hemicellulase with the mass percentage concentration of 1-2% into each 100g of solid, carrying out enzymatic treatment for 1-2 h at the pH of 4-7 and the temperature of 30-40 ℃, inactivating for 1min at the temperature of 80-100 ℃, extracting an enzymolysis extracting solution again by adopting a flash type rapid extraction method, filtering to obtain an extracting solution, and concentrating; the ratio of AB-8: purifying the mixed purification material LH-20 (1: 0.2-5) by a mixed resin method, performing gradient elution by using 10-90% ethanol-water by volume percentage to obtain an extract 2 concentrated solution, freeze-drying, crushing, and sieving by using a 80-mesh sieve to obtain an extract 2;
the extraction of the extract 3 was carried out according to the following steps: crushing codonopsis pilosula, ligusticum wallichii, dandelion, whole plants, roasted malt, fructus evodiae and Chinese date, taking 5-40% ethanol-water in percentage by volume as an extraction solvent, adding cellulase with the mass percentage concentration of 2-4% and hemicellulase with the mass percentage concentration of 1-2% into each 100g of solid at the solid-liquid volume ratio of 1: 10-20 and the temperature of 30-40 ℃, and performing enzymatic treatment for 2-4 hours; performing microwave treatment for 2-4 min at the microwave power of 10-30 KW; transferring the microwave system into an ultrasonic extractor, and extracting the functional additive at an ultrasonic extraction temperature of 50-80 ℃ for 30-60 min at an ultrasonic frequency of 20-30 kHz; recovering the ethanol solvent, concentrating to 1/8-1/4 of the original volume, and spray drying to obtain the extract 3;
the extraction of the extract 4 was carried out according to the following steps: crushing black rice, taking black rice peel, adding amylase with the mass percentage concentration of 1-5% into every 100g of solid, performing enzymatic treatment for 2-4 hours, performing microwave treatment for 1-2 min at the microwave power of 10-20 KW; taking a hydrochloric acid aqueous solution with the volume percentage content of 0.1-1% as a solvent, carrying out ultrasonic treatment at the temperature of 30-50 ℃, carrying out ultrasonic treatment at the power of 200-800W for 60-120 min, extracting for 2 times, concentrating the obtained extracting solution at low temperature to the original volume of 1/5-1/10, pre-freezing at the temperature of-20 ℃, and carrying out freeze drying; with D101: AB-8: performing gradient elution on the D201-1: 1: 1-5 ethanol-water system with the volume percentage of 10-90% to obtain an active ingredient, and performing freeze drying to obtain the extract 4;
taking the extract 5 according to the following steps: adding absolute ethyl alcohol into honey to enable the volume content of the ethyl alcohol to exceed 70-90%, adding 5-10% of sorbitol according to 100 g: and (2) carrying out ultrasonic treatment on xylitol at the ratio of 1:1 for 5-10 min, carrying out centrifugal filtration, concentrating and spray-drying an alcohol extraction part for later use, carrying out pre-freezing on a non-alcohol-soluble part at the temperature of-20 ℃, carrying out freeze-drying, and mixing the freeze-dried powder and a spray-dried product to obtain the extract 5.
6. A process for the preparation of an effervescent tablet as claimed in claim 2 or 3, comprising the steps of:
mixing the extract 1, the extract 3, the extract 4 and the extract 5 with the citric acid and the xylitol, adding a binding agent, and performing granulation, drying and size stabilization to obtain an effervescent acid phase; mixing the extract 2 with the sodium bicarbonate and the sodium alginate, and performing dry granulation and granule finishing to obtain an effervescent base phase; and mixing the effervescent acid phase and the effervescent alkali phase, adding the lubricant, mixing, tabletting and packaging to obtain the effervescent tablet.
7. Use of an effervescent tablet as claimed in any one of claims 1 to 6 in the preparation of a product having the following functions 1) to 3):
1) auxiliary protection of gastric mucosa;
2) at least one active function of antioxidation, antifatigue and auxiliary hypoglycemic;
3) protecting liver.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910409294.6A CN110150528B (en) | 2019-05-16 | 2019-05-16 | Effervescent tablet and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910409294.6A CN110150528B (en) | 2019-05-16 | 2019-05-16 | Effervescent tablet and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110150528A CN110150528A (en) | 2019-08-23 |
| CN110150528B true CN110150528B (en) | 2022-09-16 |
Family
ID=67630997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910409294.6A Active CN110150528B (en) | 2019-05-16 | 2019-05-16 | Effervescent tablet and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110150528B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103356933B (en) * | 2013-07-18 | 2015-05-27 | 苏州市天灵中药饮片有限公司 | Stomach-nourishing spleen-strengthening chewing tablets and preparation method thereof |
| CN104382012A (en) * | 2014-10-28 | 2015-03-04 | 广西金昊生物科技有限公司 | Nutritional powder containing okra, and preparation method of nutritional powder |
| CN104473159B (en) * | 2014-11-26 | 2016-04-06 | 牡丹江师范学院 | A kind of silvervine effervescent agent and preparation method thereof and application |
| CN106307488A (en) * | 2016-08-16 | 2017-01-11 | 贾陆 | Full-nutrient instant porridge powder containing abelmoschus esculentus and preparation method thereof |
| CN107373550A (en) * | 2017-08-30 | 2017-11-24 | 宿松县美智农林发展有限公司 | A kind of strengthening spleen and nourishing stomach tara vine jam |
-
2019
- 2019-05-16 CN CN201910409294.6A patent/CN110150528B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110150528A (en) | 2019-08-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104248666A (en) | Compositions for preventing or improving gastrointestinal diseases | |
| CN101361733B (en) | Use of flavone derivates and preparation method thereof | |
| CN103652360B (en) | Feed for curing pig paratyphus and preparation method thereof | |
| CN101637492B (en) | Preparation method of total flavonoids in persimmon leaves | |
| CN113480676B (en) | Oligogalacturonic acid polysaccharide, compound, preparation method and application thereof | |
| CN104982597B (en) | A kind of multi-functional composite instant tea and its preparation method and application | |
| CN104857154A (en) | Traditional Chinese medicine composition for treating three-high diseases and preparation method therefor | |
| CN110150528B (en) | Effervescent tablet and preparation method and application thereof | |
| JP4516958B2 (en) | Anti-diabetic composition | |
| CN110051801B (en) | Chewable tablet and preparation method and application thereof | |
| CN110326788A (en) | A kind of bitter buckwheat d-chiro-inositol compound hypoglycemic tabletting and preparation method thereof | |
| CN109248188A (en) | A kind of preparation method and applications of goldspink root extract | |
| CN107812115A (en) | A kind of Chinese medicine composition for treating diabetes | |
| CN104367612B (en) | A kind of application of dog ant grass extract | |
| CN106928376A (en) | The separation method of skunk bush polysaccharide and its application | |
| JP3208531B2 (en) | Cosmetics containing grape and ginseng browned compositions | |
| CN102784183B (en) | The preparation method of a kind of Radix Ginseng Rubra polysaccharide extract product | |
| CN110090243A (en) | A kind of Semen euryales extract for Postprandial glucose control, preparation method and application | |
| KR20200120801A (en) | Red Ginseng Extract comprising Saponin and high purity Acidic Polysaccarride, Manufacturing method thereof and Healty Food containing the same | |
| KR101161636B1 (en) | Red ginseng extract containing Liriope platyphyllla and producing method thereof | |
| JP4636475B2 (en) | Antidiabetic | |
| CN109662236A (en) | A kind of food additives of the more saponin(es of selenium-rich polysaccharide, preparation method and applications | |
| KR20200061446A (en) | Food composition using the salicornia herbacea and method for producing the same | |
| KR100391195B1 (en) | a manufacturing technique of a beverage using of an educt which is distilled from Liriope spicata Lour | |
| CN103656355B (en) | Medicine being used for the treatment of swine paratyphoid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230625 Address after: 515638 Middle section of Daxia Road, Chao'an District, Chaozhou, Guangdong Province Patentee after: Guangdong Haiyi Health Technology Co.,Ltd. Address before: No. 191, Wenhua street, Aimin District, Mudanjiang City, Heilongjiang Province, 157011 Patentee before: Mudanjiang Normal University |
|
| TR01 | Transfer of patent right |