CN110150369B - Deoxidation and preservation method for pleurotus eryngii - Google Patents

Deoxidation and preservation method for pleurotus eryngii Download PDF

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Publication number
CN110150369B
CN110150369B CN201910518843.3A CN201910518843A CN110150369B CN 110150369 B CN110150369 B CN 110150369B CN 201910518843 A CN201910518843 A CN 201910518843A CN 110150369 B CN110150369 B CN 110150369B
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pleurotus eryngii
iron powder
deoxidizer
deoxidizing
packaging bag
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CN110150369A (en
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千春录
李飞
金昌海
刘俊
阚娟
张年凤
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Jiangsu Bokang Fresh Mushroom Co ltd
Yangzhou University
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Jiangsu Bokang Fresh Mushroom Co ltd
Yangzhou University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/04Freezing; Subsequent thawing; Cooling
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/144Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A23B7/148Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere, e.g. partial vacuum, comprising only CO2, N2, O2 or H2O

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
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  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Abstract

The invention relates to a method for deoxidizing and preserving pleurotus eryngii, and belongs to the technical field of edible mushroom preservation. The method comprises the following steps of (1) activating iron powder; (2) activated iron powder slow release: the activated iron powder is wrapped by hydrophobically modified sodium alginate; (3) preparing a deoxidizing agent: the deoxidizer is an iron-based deoxidizer; (4) packaging a deoxidizer: filling the prepared deoxidizer into a small breathable and perforated packaging bag with the length of 4cm and the width of 3 cm; (5) pre-cooling raw materials: placing the pleurotus eryngii in an environment with the temperature of 8 ℃ for precooling for 24 hours; (6) subpackaging raw materials: subpackaging the pre-cooled loquat into transparent polyethylene packaging bags; (7) deoxidizing and packaging; (8) and (4) storing at low temperature. The preservation method can obviously prolong the refrigeration period of the pleurotus eryngii and better maintain the original flavor and taste of the pleurotus eryngii. The method is simple to operate, safe, nontoxic, low in cost and wide in market application prospect.

Description

Deoxidation and preservation method for pleurotus eryngii
Technical Field
The invention relates to a method for deoxidizing and preserving pleurotus eryngii, and belongs to the technical field of edible mushroom preservation.
Background
Oxygen scavengers (Oxygen scavenger) are a new technology currently being studied and used in the field of fresh food preservation, and can consume free Oxygen or combined Oxygen in the air through the reaction of chemical components. The deoxidizer is sealed with food to prevent food from oxidative discoloration and inhibit the growth of aerobic and aerobe. The commonly used deoxidizer is an iron-based deoxidizer, which has high efficiency and low cost, and the deoxidation reaction can be rapidly carried out under the conditions of water and oxygen. The deoxidation package of fresh fruits and vegetables is less in application, because a large amount of water can be released under the respiratory action in the space in the package, the deoxidation reaction is rapidly carried out, and a large amount of deoxidizing agent is consumed, so that the deoxidation effect in the later storage period is poor.
Pleurotus eryngii is a fungus used as both medicine and food and is a beautiful name of "grassland Boletus edulis". The pleurotus eryngii has bright color, white color, no spot and delicious taste and is deeply loved by the consumers. The pleurotus eryngii is rich in nutrition, and contains low fat and high protein, various amino acids necessary for human bodies, and trace elements such as iron, zinc and the like. Pleurotus eryngii is a precious fungus, has high metabolic activity after being picked, can consume a large amount of nutrients in a body, and causes quality reduction, which is represented by weight loss, opening of a pileus, growth of stipe, water immersion of tissues and browning. The conventional preservation technology does not achieve the ideal effect, so a preservation technology suitable for storing the picked pleurotus eryngii needs to be found, and the deoxidation preservation technology of the pleurotus eryngii is not reported at present.
The patent (publication No. CN105394167A, publication No. 2016, 03, 16) discloses a pleurotus eryngii preservative and a preparation method thereof, and the patent (publication No. CN107258891A, publication No. 2017, 10, 20) discloses a pleurotus eryngii preservation processing technology, which indicates the pleurotus eryngii preservation method. Generally, the antistaling agent is prepared and directly and uniformly sprayed on the surface of the pleurotus eryngii, but the antistaling agent is directly and independently packaged and sealed together with the pleurotus eryngii.
The method for deoxidizing and refreshing pleurotus eryngii can effectively and durably reduce the closed inner space O2And CO2The proportion of the bacillus subtilis reduces the respiration and the postharvest metabolic process of the pleurotus eryngii, inhibits the postharvest disease occurrence and the growth of microorganisms of the pleurotus eryngii, and obviously prolongs the storage period of the pleurotus eryngii. The deoxidation preservation has low cost, simple process and good preservation effect, and can ensure the storage quality of the pleurotus eryngii in the storage and commercial circulation processes.
Disclosure of Invention
The invention aims to provide a method for deoxidizing and preserving pleurotus eryngii, which solves the problems that the storage period of the pleurotus eryngii after being picked is short, preservative residues possibly have adverse effects on human bodies and the like.
The invention aims to realize the method for deoxidizing and refreshing pleurotus eryngii, and is characterized in that precooled pleurotus eryngii is directly filled into a polyethylene packaging bag, a slow-release deoxidizer is added into the polyethylene packaging bag, the polyethylene packaging bag is sealed, and the polyethylene packaging bag is refrigerated in a refrigerator to realize the deoxidizing and refreshing of the pleurotus eryngii.
The sustained-release deoxidizer added into the polyethylene packaging bag is 1-pack sustained-release deoxidizer, and the preparation method of the sustained-release deoxidizer comprises the following steps:
(1) activating iron powder;
mixing 30g of reduced iron powder with 200mL of hydrochloric acid with the concentration of 5%, boiling for 10min, carrying out suction filtration, washing with deionized water to be neutral, and drying in a vacuum drying oven at 70 ℃ and 110 Pa for 1h after suction filtration to obtain activated iron powder;
(2) the activated iron powder is slowly released;
dissolving 1.5g of sodium alginate, 1.0g of hydroxymethyl cellulose (CMC) and 0.3g of glycerol into 100mL of phosphate buffer solution with pH value of 6.0, magnetically stirring at constant temperature of 70 ℃ until the sodium alginate, degassing at 0.095MPa by using a vacuum pump to prepare a sodium alginate solution, adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC & HCl)1g and N-hydroxysuccinimide (NHS)0.3g, stirring at 70 ℃ for 30min, adding 0.3g of Ethylenediamine (EDA), stirring for 2h, adding 30g of activated iron powder, stirring for 30min, carrying out suction filtration, wetting the iron powder, drying in a vacuum drying box with temperature of 40 ℃ and pressure of 110 Pa to obtain the slow-release activated iron powder, wherein the surface of the iron powder is covered with a hydrophobic sodium alginate film;
(3) preparing a deoxidizing agent;
uniformly mixing 1-1.5 g of activated iron powder, 1-2g of slow-release activated iron powder, 0.3-0.6 g of NaCl0.15-0.5 g of activated carbon and 0.35-1 g of diatomite to obtain the deoxidizer.
(4) Packaging deoxidizer;
and filling the prepared deoxidizer into a small breathable and perforated packaging bag with the length of 4cm and the width of 3cm, wherein the packaging bag is made of a PET/PE composite material, the diameter of each hole is less than 0.5mm, the content is ensured not to leak, and the density of the holes is about 8/cm 2, so that the packaged slow-release deoxidizer is obtained.
The pleurotus eryngii precooling is to place the pleurotus eryngii in an environment with the temperature of 8 ℃ for precooling for 24 hours to obtain precooled pleurotus eryngii.
The polyethylene packaging bag has the length of 15cm, the width of 10cm and the thickness of 12 mu m, and is O2A transmittance of 2.87X 10-10 mL/cm/(m 2 s Pa); each bag of the polyethylene packaging bags is 500g of pleurotus eryngii.
The polyethylene packaging bag is sealed in a heat sealing mode.
The temperature in the refrigeration house is 4 ℃ and the humidity is 75%.
The method is advanced and scientific, is convenient to use, and is realized by the following technical scheme, and the method for deoxidizing and preserving the pleurotus eryngii specifically comprises the following steps:
step (1), iron powder activation: mixing 30g of reduced iron powder with 200mL of hydrochloric acid with the concentration of 5%, boiling for 10min, carrying out suction filtration, washing with deionized water to be neutral, and drying in a vacuum drying oven at 70 ℃ and 110 Pa for 1h after suction filtration to obtain the activated iron powder.
Step (2), activated iron powder slow release: 1.5g of sodium alginate, 1.0g of hydroxymethyl cellulose (CMC) and 0.3g of glycerol are dissolved in 100mL of phosphate buffer solution with pH of 6.0, the mixture is magnetically stirred at the constant temperature of 70 ℃ until the mixture is dissolved, and a vacuum pump is used for degassing under the pressure of 0.095MPa to prepare the sodium alginate solution. Adding 1g of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC & HCl) and 0.3g of N-hydroxysuccinimide (NHS), stirring for 30min at 70 ℃, adding 0.3g of Ethylenediamine (EDA), stirring for 2h, adding 30g of activated iron powder, stirring for 30min, performing suction filtration on the wet iron powder, and drying in a vacuum drying box at 40 ℃ and 110-130pa to obtain the slow-release activated iron powder, wherein the surface of the iron powder is covered with a hydrophobic sodium alginate film.
Step (3), preparation of a deoxidizer: 1-1.5 g of activated iron powder, 1-2g of slow-release activated iron powder, 0.3-0.6 g of NaCl, 0.15-0.5 g of activated carbon and 0.35-1 g of diatomite, and uniformly mixing the components to obtain the deoxidizer;
step (4), deoxidizer packaging: filling the prepared deoxidizer into a small breathable and perforated packaging bag with the length of 4cm and the width of 3cm, wherein the packaging bag is made of a PET/PE composite material, the diameter of each hole is less than 0.5mm, the content is ensured not to leak, and the density of each hole is about 8/cm 2;
step (5), pre-cooling raw materials: placing the pleurotus eryngii in an environment with the temperature of 8 ℃ for precooling for 24 hours;
step (6), split charging of raw materials: subpackaging the precooled pleurotus eryngii into transparent polyethylene packaging bags, wherein each bag is 500g in weight; wherein the transparent polyethylene packaging bag has a length of 15cm, a width of 10cm, a thickness of 12 μm, and O2A transmittance of 2.87X 10-10 mL/cm/(m 2 s Pa);
and (7) deoxidizing and packaging: adding a package of the deoxidizer packaged in the step (2) into a transparent polyethylene packaging bag filled with pleurotus eryngii, and then carrying out heat sealing;
step (8), low-temperature storage: storing the pleurotus eryngii subjected to deoxidization packaging in a refrigeration house with the temperature of 4 ℃ and the humidity of 75 percent.
The invention relates to a method for deoxidizing and preserving pleurotus eryngii, and belongs to the technical field of edible mushroom preservation. The method comprises the following steps of (1) activation of iron powder, (2) slow release of activated iron powder, (3) allocation of a deoxidizer, (4) packaging of the deoxidizer, (5) precooling of raw materials, (6) subpackaging of the raw materials, (7) deoxidation packaging and (8) low-temperature storage. The preservation method can remarkably prolong the refrigeration period of the pleurotus eryngii and better maintain the original flavor and taste of the pleurotus eryngii. The method is simple to operate, safe, nontoxic, low in cost and wide in market application prospect.
The invention mainly utilizes the deoxidizer to prolong the storage period of the pleurotus eryngii, and the deoxidized and packaged pleurotus eryngii maintains better post-harvest quality. Compared with the prior art, the method for independently packaging the small package by adding the deoxidizer has the following advantages that:
the method for deoxidizing and preserving the pleurotus eryngii effectively adjusts the closed packaging space O2And CO2In a ratio of (1) to (2) that can rapidly reduce O in the package2The content of the pleurotus eryngii is high, the hypoxic state can be maintained for a long time, the respiration of the pleurotus eryngii is reduced, the postharvest diseases of the pleurotus eryngii and the growth of microorganisms are inhibited, and the storage period of the pleurotus eryngii is prolonged remarkably. The method has noThe method has the advantages of toxicity, harmlessness, simple operation and low cost, and fully ensures the storage quality and the nutritive value of the pleurotus eryngii. During the low-temperature storage period, the oxygen level is quickly and durably reduced by adding the deoxidizer into the packaging bag filled with the pleurotus eryngii and independently packaging, and the method shows great superiority compared with the method of spraying the antistaling agent on the surface of the pleurotus eryngii by the traditional method.
Drawings
FIG. 1 is a diagram of the refrigeration effect of Pleurotus eryngii.
Detailed Description
The following describes the technical solution in the examples of the present invention in detail.
Example 1: the deoxidizer contains the following components: 1g of activated iron powder, 1g of slow-release iron powder, 0.4g of NaCl, 0.9g of activated carbon and 0.9g of diatomite, and the prepared deoxidizer is filled into a small packaging bag with the length of 4cm and the width of 3 cm. Selecting pleurotus eryngii without obvious mechanical damage and plant diseases and insect pests, and pre-cooling the pleurotus eryngii in an environment with the temperature of 8 ℃ for 24 hours. And subpackaging the precooled pleurotus eryngii into polyethylene transparent plastic bags, wherein each bag is 500g in weight. Respectively adding a packet of deoxidizer independent small package (each bag is 4.2g) into a polyethylene transparent plastic bag filled with pleurotus eryngii, tying and sealing the bag, wherein the volume of residual air in the polyethylene plastic bag is about 800 mL. The pleurotus eryngii after being sealed and packaged is stored in a refrigerator with the temperature of 4 ℃ and the humidity of 75 percent.
Example 2: the deoxidizer contains the following components: 1.2g of activated iron powder, 1.5g of slow-release iron powder, 0.5g of NaCl, 0.4g of activated carbon and 0.6g of diatomite, and the prepared deoxidizer is filled into a small packaging bag with the length of 4cm and the width of 3 cm. Selecting pleurotus eryngii without obvious mechanical damage and plant diseases and insect pests, and pre-cooling the pleurotus eryngii in a refrigeration house at the temperature of 8 ℃ for 24 hours. And subpackaging the precooled pleurotus eryngii into polyethylene transparent plastic bags, wherein each bag is 500g in weight. Respectively adding a packet of deoxidizer independent small package (each bag is 4.2g) into a polyethylene transparent plastic bag filled with pleurotus eryngii, tying and sealing the bag, wherein the volume of residual air in the polyethylene plastic bag is about 800 mL. The pleurotus eryngii after being sealed and packaged is stored in a refrigerator with the temperature of 4 ℃ and the humidity of 75 percent.
Example 3: the deoxidizer contains the following components: 1.5g of iron powder, 2g of slow-release iron powder, 0.5g of NaCl, 0.1g of activated carbon and 0.1g of diatomite, and the prepared deoxidizer is filled into a small packaging bag with the length of 4cm and the width of 3 cm. Selecting pleurotus eryngii without obvious mechanical damage and plant diseases and insect pests, and pre-cooling the pleurotus eryngii in a refrigeration house at the temperature of 8 ℃ for 24 hours. And subpackaging the precooled pleurotus eryngii into polyethylene transparent plastic bags, wherein each bag is 500g in weight. Respectively adding a packet of deoxidizer independent small package (each bag is 4.2g) into a polyethylene transparent plastic bag filled with pleurotus eryngii, tying and sealing the bag, wherein the volume of residual air in the polyethylene plastic bag is about 800 mL. The pleurotus eryngii after being sealed and packaged is stored in a refrigerator with the temperature of 4 ℃ and the humidity of 75 percent.
Storage experiments were carried out on three kinds of de-oxygenated, fresh-preserved pleurotus eryngii according to examples 1 to 3 and a control pleurotus eryngii without any treatment, stored in the same environment for 35 days, and 20 experienced judges were selected for sensory evaluation. Table 1 shows the sensory evaluation results of the deoxidized and preserved pleurotus eryngii of the present invention and those of pleurotus eryngii without any treatment.
TABLE 1 sensory evaluation criteria and results for Pleurotus eryngii
Figure BDA0002095971380000051
As can be seen from Table 1 and FIG. 1, compared with the control group which is not treated, the three kinds of deoxygenated and packaged pleurotus eryngii of the invention are subjected to sensory evaluation after being refrigerated for 35d, and the pleurotus eryngii packaged by the deoxygenation of the invention is found to be obviously improved in color, smell, taste and texture, and mainly shows that the pleurotus eryngii stored by the preservation technology of the invention has normal color, no browning phenomenon and good elasticity, so that the deoxygenated and preservation technology of the invention can well store and transport the pleurotus eryngii.
The embodiments of the present invention have been described in detail above, but this is only an example for easy understanding and should not be construed as limiting the scope of the present invention. Also, various equivalent changes or substitutions are possible for those skilled in the art according to the technical solution of the present invention and the description of the preferred embodiment thereof, and all such changes or substitutions shall fall within the protection scope of the claims of the present invention.

Claims (5)

1. A method for deoxidizing and refreshing pleurotus eryngii is characterized in that precooled pleurotus eryngii is directly filled into a polyethylene packaging bag, a slow-release deoxidizing agent is added into the polyethylene packaging bag, the polyethylene packaging bag is sealed, and the polyethylene packaging bag is refrigerated in a refrigeration house to realize the deoxidizing and refreshing of the pleurotus eryngii;
the preparation method of the slow-release deoxidizer comprises the following steps:
(1) activating iron powder;
mixing 30g of reduced iron powder with 200mL of hydrochloric acid with the concentration of 5%, boiling for 10min, carrying out suction filtration, washing with deionized water to be neutral, and drying in a vacuum drying oven at 70 ℃ and 110 Pa for 1h after suction filtration to obtain activated iron powder;
(2) the activated iron powder is slowly released;
dissolving 1.5g of sodium alginate, 1.0g of hydroxymethyl cellulose (CMC) and 0.3g of glycerol into 100mL of phosphate buffer solution with pH of 6.0, magnetically stirring at constant temperature of 70 ℃ until the sodium alginate, degassing at 0.095MPa by using a vacuum pump to prepare a sodium alginate solution, adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC.HCl) 1g and N-hydroxysuccinimide (NHS)0.3g, stirring at 70 ℃ for 30min, adding 0.3g of Ethylenediamine (EDA), stirring for 2h, adding 30g of activated iron powder, stirring for 30min, carrying out suction filtration, moistening the iron powder, drying in a vacuum drying oven at 40 ℃ and 110-130pa to obtain the slow-release activated iron powder, wherein the surface of the iron powder is covered with a hydrophobic sodium alginate film;
(3) preparing a deoxidizing agent;
uniformly mixing 1-1.5 g of activated iron powder, 1-2g of slow-release activated iron powder, 0.3-0.6 g of NaCl0.15-0.5 g of activated carbon and 0.35-1 g of diatomite to obtain a deoxidizer;
(4) packaging a deoxidizer;
filling the prepared deoxidizer into a small breathable and perforated packaging bag with the length of 4cm and the width of 3cm, wherein the packaging bag is made of PET/PE composite material, the diameter of each hole is less than 0.5mm, the content is ensured not to leak, and the density of each hole is about 8 holes/cm2And obtaining the packaged slow-release deoxidizer.
2. The method for deoxidizing and refreshing pleurotus eryngii according to claim 1, wherein the pleurotus eryngii precooling is performed by placing the pleurotus eryngii in an environment with the temperature of 8 ℃ for 24 hours to obtain precooled pleurotus eryngii.
3. The method for deoxidizing and refreshing pleurotus eryngii according to claim 1, wherein the polyethylene packaging bag has a length of 15cm, a width of 10cm and a thickness of 12 μm, and is O2A transmission coefficient of 2.87X 10−10mL·cm/(m2S · Pa); each bag of the polyethylene packaging bags is 500g of pleurotus eryngii.
4. The deoxygenation and preservation method of Pleurotus eryngii according to claim 1, wherein the polyethylene packaging bag is sealed by heat sealing.
5. The method for deoxidizing and refreshing pleurotus eryngii according to claim 1, wherein the temperature in the refrigerator is 4 ℃ and the humidity is 75%.
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CN109793043A (en) * 2019-03-11 2019-05-24 扬州大学 A kind of preservation method of loquat Deoxygen Packaging

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