CN110100978A - A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility - Google Patents
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility Download PDFInfo
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- CN110100978A CN110100978A CN201910457320.2A CN201910457320A CN110100978A CN 110100978 A CN110100978 A CN 110100978A CN 201910457320 A CN201910457320 A CN 201910457320A CN 110100978 A CN110100978 A CN 110100978A
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Abstract
The invention discloses the research methods that a kind of yellow meal worm replaces fish meal raising fancy carp feasibility, its method includes experimental material preparation-feed preparation-experiment preparation-experiment management-sample detecting-data processing and statistical analysis, fancy carp is raised with yellow meal worm substitution fish meal in this test, by studying its influence to fancy carp non-specificity index, Antioxidant Indexes and anti-inflammatory factors, to be yellow meal worm in terms of aquaculture nutrition with feed application provide fundamental basis.
Description
Technical field
The present invention relates to agriculture fields, and the research side of fish meal raising fancy carp feasibility is replaced more particularly to a kind of yellow meal worm
Method.
Background technique
Fancy carp alias is Japanese kampo, red carp.It is a kind of fish being evolved by carp, is biologically belonging to carp
Section fish.The body colour of own can change with the variation of breeding environment.Its posture is plump, body colour is bright-coloured, has a sweet temper, product
Kind is abundant, has obtained the welcome of vast ornamental fish fan.
Yellow meal worm is under the jurisdiction of the Insecta that animal kingdom's most species, quantity are maximum, are distributed in most wide Arthropoda, people
It is referred to as bread worm mostly.It originally is a kind of storage pest of large size, nowadays widely distributed is mostly the animality egg of high protein
The insect of Bai Yuan exists.Yellow meal worm not only has important role in physiology and genetically, but also even more protein content is very high excellent
Matter insects product [1].The feeding habits of yellow meal worm are wide and omnivorous, and the limitation of climate and region, not of less demanding to environmental condition,
Easily raising, thus it is extensive in aquaculture industrial application.Crude protein content is up to 60% or so, dry weight cost after yellow meal worm drying
3/5 or so of fish meal prices.Some researches show that directly feed fresh yellow meal worm or feed appropriate addition 50% fish meal of content
The yellow meal worm of mixed feed can rouge in normal growth to it, the fat content of intake and muscle to Spinibarbus sinensis
Fat content has apparent influence, also has different degrees of improvement to the delicious degree and quality of muscle.
The main problem that limit aquaculture sustainable development is the supply of fish meal, and domestic fish meal and imported fish meal are
The main two kinds of sources of the fish meal of Chinese market, but because the variation of climatic environment all can not normally protect fish meal on quality and quantity
Card.Therefore, mitigate and fish meal is depended on unduly in aquaculture, seek suitable high protein insect to replace fish meal to become
Our extremely urgent problems to be solved.There are many scholars to further investigate, and Lee's Yao Yao etc. substitutes albumen yellow powder
Fish meal observes the influence to young turbot, obtains optimal additive amount 25% or so.
Fancy carp is raised with yellow meal worm substitution fish meal in the present invention, by studying it to fancy carp non-specificity index, anti-oxidant
The influence of index and anti-inflammatory factors, to be yellow meal worm in terms of aquaculture nutrition with feed application provide fundamental basis.
Summary of the invention
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
Feed prepares
This test designs 6 groups of feeds altogether, and G1, G2, G3, G4, G5, G6, wherein G1 is control group, substitution fish meal ratio point
Not Wei (0%, 20%, 40%, 60%, 80%, 100%), after mixing with the raw material in formula, be squeezed into particle with granulator
Expect, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
Experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment；
Experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
Sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
Data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
Preferably, the material composition in step (2) formula includes dark fish meal, yellow meal worm, shrimp med, wheat, dregs of beans, face
Powder, Gluten, starch, soya-bean oil, multivitamin, several mineral materials, vegetable seed, corn, crude protein, crude fat, ash content and moisture.
Preferably, the G1 composition of raw materials are as follows: 15.0% dark fish meal, 7.0% shrimp med, 7.3% wheat, 25.5% dregs of beans,
10.0% flour, 12.0% Gluten, 2.0% starch, 3.2% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials,
9.0% vegetable seed, 4.0% corn, 50.8% crude protein, 17.1% crude fat, 7.9% ash content and 23.9% moisture.
Preferably, the G2 composition of raw materials are as follows: 12.0% dark fish meal, 3.0% yellow meal worm, 7.0% shrimp med, 6.4% wheat,
27.0% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 2.6% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.2% crude protein, 18.2% crude fat, 8.0% ash content and 23.1% water
Point.
Preferably, the G3 composition of raw materials are as follows: 9.0% dark fish meal, 6.0% yellow meal worm, 7.0% shrimp med, 5.5% wheat,
28.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.9% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.4% crude protein, 18.0% crude fat, 8.1% ash content and 23.9% water
Point.
Preferably, the G4 composition of raw materials are as follows: 6.0% dark fish meal, 9.0% yellow meal worm, 7.0% shrimp med, 4.7% wheat,
30.0% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.3% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.6% crude protein, 18.2% crude fat, 8.0% ash content and 23.0% water
Point.
Preferably, the G5 composition of raw materials are as follows: 3.0% dark fish meal, 12.0% yellow meal worm, 7.0% shrimp med, 3.9% wheat,
31.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 0.6% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.3% crude protein, 17.4% crude fat, 7.9% ash content and 24.0% water
Point.
Preferably, the G6 composition of raw materials are as follows: 15.0% yellow meal worm, 7.0% shrimp med, 3.5% wheat, 32.5% dregs of beans,
10.0% flour, 12.0% Gluten, 2.0% starch, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed,
4.0% corn, 50.7% crude protein, 17.1% crude fat, 8.0% ash content and 24.0% moisture.
Preferably, step (3) the breeding environment manufacturing method be cultivating water box in advance for 24 hours with potassium permanganate solution into
The processing of row soaking disinfection, breeding water are shifted to an earlier date 72h aeration, are persistently oxygenated using gas stone.
Preferably, start to test after taming 10d under conditions of step (3) the Experimental fish physical efficiency normal physiological activity.
The utility model has the advantages that the present invention provides the research method that a kind of yellow meal worm replaces fish meal raising fancy carp feasibility, side
Method includes experimental material preparation-feed preparation-experiment preparation-experiment management-sample detecting-data processing and statisticallys analyze, this
Fancy carp is raised with yellow meal worm substitution fish meal in test, by studying it to fancy carp non-specificity index, Antioxidant Indexes and anti-inflammatory
The influence of the factor, to be yellow meal worm in terms of aquaculture nutrition with feed application provide fundamental basis, the step (2)
Material composition in formula includes dark fish meal, yellow meal worm, shrimp med, wheat, dregs of beans, flour, Gluten, starch, soya-bean oil, Duo Zhongwei
Raw element, several mineral materials, vegetable seed, corn, crude protein, crude fat, ash content and moisture；The G1 composition of raw materials are as follows: 15.0% is brown
Fish meal, 7.0% shrimp med, 7.3% wheat, 25.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 3.2% beans
Oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.8% crude protein, 17.1% are slightly
Fat, 7.9% ash content and 23.9% moisture；The G2 composition of raw materials are as follows: 12.0% dark fish meal, 3.0% yellow meal worm, 7.0% shrimp
Powder, 6.4% wheat, 27.0% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 2.6% soya-bean oil, more than 1.0% kind
Vitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.2% crude protein, 18.2% crude fat, 8.0% ash
Point and 23.1% moisture；The G3 composition of raw materials are as follows: 9.0% dark fish meal, 6.0% yellow meal worm, 7.0% shrimp med, 5.5% wheat,
28.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.9% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.4% crude protein, 18.0% crude fat, 8.1% ash content and 23.9% water
Point；The G4 composition of raw materials are as follows: 6.0% dark fish meal, 9.0% yellow meal worm, 7.0% shrimp med, 4.7% wheat, 30.0% dregs of beans,
10.0% flour, 12.0% Gluten, 2.0% starch, 1.3% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials,
9.0% vegetable seed, 4.0% corn, 50.6% crude protein, 18.2% crude fat, 8.0% ash content and 23.0% moisture；The G5 is former
Material formula are as follows: 3.0% dark fish meal, 12.0% yellow meal worm, 7.0% shrimp med, 3.9% wheat, 31.5% dregs of beans, 10.0% flour,
12.0% Gluten, 2.0% starch, 0.6% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed,
4.0% corn, 50.3% crude protein, 17.4% crude fat, 7.9% ash content and 24.0% moisture；The G6 composition of raw materials are as follows:
15.0% yellow meal worm, 7.0% shrimp med, 3.5% wheat, 32.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% form sediment
Powder, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.7% crude protein, 17.1% are slightly
On the one hand fat, 8.0% ash content and 24.0% moisture can ensure fancy carp growth institute by the feed ingredient of above each experimental group
On the other hand the difference of yellow meal worm content is arranged in the nutritional ingredient needed by single-factor, reach research yellow meal worm substitution fish meal
Effect, step (3) the breeding environment manufacturing method are that cultivating water box carries out soaking disinfection with potassium permanganate solution for 24 hours in advance
Processing, breeding water are shifted to an earlier date 72h aeration, are persistently oxygenated using gas stone, which, which can remove, is unfavorable for fancy carp in environment
The factor of growth utmostly guarantees single-factor variable, the i.e. change of yellow meal worm content, so that result of study is more scientific,
Start to test after taming 10d under conditions of step (3) the Experimental fish physical efficiency normal physiological activity, this method enables to brocade
Carp adapts to experiment growing environment, prevents unexpected environment change from impacting to fancy carp growth.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
Embodiment 1:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
Feed prepares
This test configures feed according to G1, and substitution fish meal ratio is respectively 0%, after mixing with the raw material in formula, uses
Granulator is squeezed into particulate material, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G1 composition of raw materials are as follows: 15.0% dark fish meal, 7.0% shrimp med, 7.3% wheat, 25.5% dregs of beans, 10.0% face
Powder, 12.0% Gluten, 2.0% starch, 3.2% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% dish
Seed, 4.0% corn, 50.8% crude protein, 17.1% crude fat, 7.9% ash content and 23.9% moisture；
Experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
Experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
Sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
Data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
Embodiment 2:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
(2) feed prepares
This test configures feed according to G2, and substitution fish meal ratio is respectively 20%, after mixing with the raw material in formula, uses
Granulator is squeezed into particulate material, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G2 composition of raw materials are as follows: 12.0% dark fish meal, 3.0% yellow meal worm, 7.0% shrimp med, 6.4% wheat, 27.0%
Dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 2.6% soya-bean oil, 1.0% multivitamin, more than 4.0% kind of mine
Substance, 9.0% vegetable seed, 4.0% corn, 50.2% crude protein, 18.2% crude fat, 8.0% ash content and 23.1% moisture；
Experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
(4) experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
(5) sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
(6) data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
Embodiment 3:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
(2) feed prepares
This test configures feed according to G3, and substitution fish meal ratio is respectively 40%, after mixing with the raw material in formula, uses
Granulator is squeezed into particulate material, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G3 composition of raw materials are as follows: 9.0% dark fish meal, 6.0% yellow meal worm, 7.0% shrimp med, 5.5% wheat, 28.5% beans
The dregs of rice, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.9% soya-bean oil, 1.0% multivitamin, 4.0% multi mineral
Matter, 9.0% vegetable seed, 4.0% corn, 50.4% crude protein, 18.0% crude fat, 8.1% ash content and 23.9% moisture；
(3) experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
(4) experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
(5) sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
(6) data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
Embodiment 4:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
(2) feed prepares
This test configures feed according to G4, and substitution fish meal ratio is respectively 60%, after mixing with the raw material in formula, uses
Granulator is squeezed into particulate material, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G4 composition of raw materials are as follows: 6.0% dark fish meal, 9.0% yellow meal worm, 7.0% shrimp med, 4.7% wheat, 30.0% beans
The dregs of rice, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.3% soya-bean oil, 1.0% multivitamin, 4.0% multi mineral
Matter, 9.0% vegetable seed, 4.0% corn, 50.6% crude protein, 18.2% crude fat, 8.0% ash content and 23.0% moisture；
(3) experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
(4) experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
(5) sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
(6) data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes
Embodiment 5:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
(2) feed prepares
This test configures feed according to G5, and substitution fish meal ratio is respectively 80%, after mixing with the raw material in formula, uses
Granulator is squeezed into particulate material, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G5 composition of raw materials are as follows: 3.0% dark fish meal, 12.0% yellow meal worm, 7.0% shrimp med, 3.9% wheat, 31.5%
Dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 0.6% soya-bean oil, 1.0% multivitamin, more than 4.0% kind of mine
Substance, 9.0% vegetable seed, 4.0% corn, 50.3% crude protein, 17.4% crude fat, 7.9% ash content and 24.0% moisture；
(3) experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
(4) experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
(5) sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
(6) data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
Embodiment 6:
A kind of yellow meal worm replaces the research method of fish meal raising fancy carp feasibility, and the method step includes:
(1) experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is standby to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body
With；
(2) feed prepares
This test configures feed according to G6, and substitution fish meal ratio is respectively 100%, after being mixed with the raw material in formula,
It is squeezed into particulate material with granulator, natural drying 7 days at non-direct sunlight, and Seal and preservation at room temperature；
The G6 composition of raw materials are as follows: 15.0% yellow meal worm, 7.0% shrimp med, 3.5% wheat, 32.5% dregs of beans, 10.0% face
Powder, 12.0% Gluten, 2.0% starch, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% jade
Rice, 50.7% crude protein, 17.1% crude fat, 8.0% ash content and 24.0% moisture；
(3) experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group 2 parallel
Group, 30 fishes of each circulating box are placed in breeding environment, and breeding environment manufacturing method is that cultivating water box uses potassium permanganate for 24 hours in advance
Aqueous solution carries out soaking disinfection processing, and breeding water is shifted to an earlier date 72h aeration, is persistently oxygenated using gas stone, Experimental fish physical efficiency is normal
Start to test after taming 10d under conditions of physiological activity；
(4) experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, quantity of exchanged water
For the 1/3-1/2 of volume, water temperature control is at 28 DEG C, culture-cycle 56d；
(5) sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing,
It takes tail vein to take a blood sample, is stood in 4 DEG C for 24 hours, Aspirate supernatant after 10min is centrifuged at 4000r/min, -80 DEG C spare, surveys
Then non-specificity index, Antioxidant Indexes and the anti-inflammatory factors index of blood plasma dissect the test fish for being pumped through blood, take its liver
Dirty, -80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made of refiner grinding
10% homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and anti-oxidant finger
Target measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT
And GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
(6) data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, difference using 17.0 software of SPSS
Significantly (P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to brocade
The influence of carp blood and liver biochemical indexes.
The sample for extracting each embodiment is tested and analyzed, and is compareed with the prior art, obtains following data:
According to above table data, it can be concluded that, when implementing embodiment 2, a kind of yellow meal worm replaces fish meal through the invention
It is 28U/gprot that the research method research acquisition parameter of raising fancy carp feasibility, which is liver ACP value, and liver AKP value is 42U/
Gprot, plasma A CP value are 28U/ml, and plasma A KP value is 27U/ml, and it is 13U/ that prior art standard, which is liver ACP value,
Gprot, liver AKP value are 24U/gprot, and plasma A CP value is 19U/ml, and plasma A KP value is 24U/ml, therefore the present invention is a kind of
The yellow meal worm substitution fish meal that yellow meal worm replaces the research method research of fish meal raising fancy carp feasibility to obtain raises fancy carp
When liver and blood plasma physicochemical data tool increase, therefore the present invention has significant superiority.
The present invention provides the research method that a kind of yellow meal worm replaces fish meal raising fancy carp feasibility, method includes experiment
Material preparation-feed preparation-experiment preparation-experiment management-sample detecting-data processing and statistical analysis, with yellow in this test
Mealworm substitutes fish meal and raises fancy carp, by studying its influence to fancy carp non-specificity index, Antioxidant Indexes and anti-inflammatory factors,
To be yellow meal worm in terms of aquaculture nutrition with feed application provide fundamental basis, the original in step (2) formula
Material ingredient includes dark fish meal, yellow meal worm, shrimp med, wheat, dregs of beans, flour, Gluten, starch, soya-bean oil, multivitamin, a variety of
Minerals, vegetable seed, corn, crude protein, crude fat, ash content and moisture；The G1 composition of raw materials are as follows: 15.0% dark fish meal, 7.0%
Shrimp med, 7.3% wheat, 25.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 3.2% soya-bean oil, more than 1.0%
Kind vitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.8% crude protein, 17.1% crude fat, 7.9%
Ash content and 23.9% moisture；The G2 composition of raw materials are as follows: 12.0% dark fish meal, 3.0% yellow meal worm, 7.0% shrimp med, 6.4% small
Wheat, 27.0% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 2.6% soya-bean oil, 1.0% multivitamin,
4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.2% crude protein, 18.2% crude fat, 8.0% ash content and
23.1% moisture；The G3 composition of raw materials are as follows: 9.0% dark fish meal, 6.0% yellow meal worm, 7.0% shrimp med, 5.5% wheat,
28.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% starch, 1.9% soya-bean oil, 1.0% multivitamin, 4.0%
Several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.4% crude protein, 18.0% crude fat, 8.1% ash content and 23.9% water
Point；The G4 composition of raw materials are as follows: 6.0% dark fish meal, 9.0% yellow meal worm, 7.0% shrimp med, 4.7% wheat, 30.0% dregs of beans,
10.0% flour, 12.0% Gluten, 2.0% starch, 1.3% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials,
9.0% vegetable seed, 4.0% corn, 50.6% crude protein, 18.2% crude fat, 8.0% ash content and 23.0% moisture；The G5 is former
Material formula are as follows: 3.0% dark fish meal, 12.0% yellow meal worm, 7.0% shrimp med, 3.9% wheat, 31.5% dregs of beans, 10.0% flour,
12.0% Gluten, 2.0% starch, 0.6% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed,
4.0% corn, 50.3% crude protein, 17.4% crude fat, 7.9% ash content and 24.0% moisture；The G6 composition of raw materials are as follows:
15.0% yellow meal worm, 7.0% shrimp med, 3.5% wheat, 32.5% dregs of beans, 10.0% flour, 12.0% Gluten, 2.0% form sediment
Powder, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.7% crude protein, 17.1% are slightly
On the one hand fat, 8.0% ash content and 24.0% moisture can ensure fancy carp growth institute by the feed ingredient of above each experimental group
On the other hand the difference of yellow meal worm content is arranged in the nutritional ingredient needed by single-factor, reach research yellow meal worm substitution fish meal
Effect, step (3) the breeding environment manufacturing method are that cultivating water box carries out soaking disinfection with potassium permanganate solution for 24 hours in advance
Processing, breeding water are shifted to an earlier date 72h aeration, are persistently oxygenated using gas stone, which, which can remove, is unfavorable for fancy carp in environment
The factor of growth utmostly guarantees single-factor variable, the i.e. change of yellow meal worm content, so that result of study is more scientific,
Start to test after taming 10d under conditions of step (3) the Experimental fish physical efficiency normal physiological activity, this method enables to brocade
Carp adapts to experiment growing environment, prevents unexpected environment change from impacting to fancy carp growth.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention
Protection scope.
Claims (10)
1. the research method that a kind of yellow meal worm replaces fish meal raising fancy carp feasibility, which is characterized in that the method step includes:
Experimental material prepares
Test is (55.49 ± 2.28) g with quality of fishes, and it is spare to purchase yellow meal worm by a length of (114.127 ± 4.06) cm of body；
Feed prepares
This test designs 6 groups of feeds altogether, and G1, G2, G3, G4, G5, G6, wherein G1 is control group, and substitution fish meal ratio is respectively
(0%, 20%, 40%, 60%, 80%, 100%) after mixing with the raw material in formula, is squeezed into particulate material with granulator, non-
Natural drying 7 days at direct sunlight, and Seal and preservation at room temperature；
Experiment preparation
Choose 360 fishes at random to be grouped, is divided into 1 control group and 5 test groups are 6 groups altogether, every group of 2 parallel groups, often
30 fishes of a circulating box are placed in breeding environment；
Experiment management
During test, daily 8:30,16:30 are respectively fed once, and feeding volume is the 3.0-5.0% of quality of fishes, and quantity of exchanged water is body
Long-pending 1/3-1/2, water temperature control is at 28 DEG C, culture-cycle 56d；
Sample detecting
Stop eating before sampling for 24 hours, 11 tail of fish is chosen from each water tank, soaks centrifuge tube and syringe with heparin when blood drawing, take
Tail vein blood sampling stands for 24 hours in 4 DEG C, Aspirate supernatant after 10min is centrifuged at 4000r/min, and -80 DEG C spare, surveys blood plasma
Non-specificity index, Antioxidant Indexes and anti-inflammatory factors index, then be pumped through blood test fish dissect, take its liver ,-
80 DEG C spare, is the physiological saline that pre-cooling is added in 1mg:9mL by mass volume ratio before measurement, is made 10% with refiner grinding
Homogenate is centrifuged 20min at 4 DEG C with 4500r/min, and supernatant is taken to carry out liver non-specificity index and Antioxidant Indexes
Measurement, this test measure 14 indexs altogether, and GSH-PX, SOD, MDA and CAT are Antioxidant Indexes, ACP, AKP, LZM, GPT and
GOT is biochemical indicator and PGF2a, C5aR, c3a, PGE2, IL-6 are anti-inflammatory factors index；
Data processing and statistical analysis
Data are arranged with 2010 software of Excel, carry out one-way analysis of variance, significant difference using 17.0 software of SPSS
(P < 0.05) carries out Multiple range test with Duncan ' s method, is as a result indicated with mean+SD, analyzes yellow meal worm to fancy carp blood
The influence of liquid and liver biochemical indexes.
2. the research method that yellow meal worm according to claim 1 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
The material composition stated in step (2) formula includes dark fish meal, yellow meal worm, shrimp med, wheat, dregs of beans, flour, Gluten, starch, beans
Oil, multivitamin, several mineral materials, vegetable seed, corn, crude protein, crude fat, ash content and moisture.
3. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G1 composition of raw materials are as follows: 15.0% dark fish meal, 7.0% shrimp med, 7.3% wheat, 25.5% dregs of beans, 10.0% flour, 12.0%
Gluten, 2.0% starch, 3.2% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% jade
Rice, 50.8% crude protein, 17.1% crude fat, 7.9% ash content and 23.9% moisture.
4. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G2 composition of raw materials are as follows: 12.0% dark fish meal, 3.0% yellow meal worm, 7.0% shrimp med, 6.4% wheat, 27.0% dregs of beans, 10.0%
Flour, 12.0% Gluten, 2.0% starch, 2.6% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% dish
Seed, 4.0% corn, 50.2% crude protein, 18.2% crude fat, 8.0% ash content and 23.1% moisture.
5. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G3 composition of raw materials are as follows: 9.0% dark fish meal, 6.0% yellow meal worm, 7.0% shrimp med, 5.5% wheat, 28.5% dregs of beans, 10.0%
Flour, 12.0% Gluten, 2.0% starch, 1.9% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% dish
Seed, 4.0% corn, 50.4% crude protein, 18.0% crude fat, 8.1% ash content and 23.9% moisture.
6. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G4 composition of raw materials are as follows: 6.0% dark fish meal, 9.0% yellow meal worm, 7.0% shrimp med, 4.7% wheat, 30.0% dregs of beans, 10.0%
Flour, 12.0% Gluten, 2.0% starch, 1.3% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% dish
Seed, 4.0% corn, 50.6% crude protein, 18.2% crude fat, 8.0% ash content and 23.0% moisture.
7. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G5 composition of raw materials are as follows: 3.0% dark fish meal, 12.0% yellow meal worm, 7.0% shrimp med, 3.9% wheat, 31.5% dregs of beans, 10.0%
Flour, 12.0% Gluten, 2.0% starch, 0.6% soya-bean oil, 1.0% multivitamin, 4.0% several mineral materials, 9.0% dish
Seed, 4.0% corn, 50.3% crude protein, 17.4% crude fat, 7.9% ash content and 24.0% moisture.
8. the research method that yellow meal worm according to claim 2 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
State G6 composition of raw materials are as follows: 15.0% yellow meal worm, 7.0% shrimp med, 3.5% wheat, 32.5% dregs of beans, 10.0% flour, 12.0%
Gluten, 2.0% starch, 1.0% multivitamin, 4.0% several mineral materials, 9.0% vegetable seed, 4.0% corn, 50.7% are slightly
Albumen, 17.1% crude fat, 8.0% ash content and 24.0% moisture.
9. the research method that yellow meal worm according to claim 1 replaces fish meal raising fancy carp feasibility, which is characterized in that institute
Stating step (3) breeding environment manufacturing method is that cultivating water box carries out soaking disinfection processing with potassium permanganate solution for 24 hours in advance, is supported
It grows and shifts to an earlier date 72h aeration with water, be persistently oxygenated using gas stone.
10. the research method that yellow meal worm according to claim 1 replaces fish meal raising fancy carp feasibility, which is characterized in that
Start to test after taming 10d under conditions of step (3) the Experimental fish physical efficiency normal physiological activity.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105166549A (en) * | 2015-09-28 | 2015-12-23 | 中海渔（福建）生物科技有限公司 | Compound feed for bred cyprinus carpio L |
| US20160135483A1 (en) * | 2014-11-18 | 2016-05-19 | National Pingtung University Of Science And Technology | Aquaculture feed formed from fermented soybean meal and earthworm meal, including the fermentation preparation method for the mixture ingredient |
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2019
- 2019-05-29 CN CN201910457320.2A patent/CN110100978A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160135483A1 (en) * | 2014-11-18 | 2016-05-19 | National Pingtung University Of Science And Technology | Aquaculture feed formed from fermented soybean meal and earthworm meal, including the fermentation preparation method for the mixture ingredient |
| CN105166549A (en) * | 2015-09-28 | 2015-12-23 | 中海渔（福建）生物科技有限公司 | Compound feed for bred cyprinus carpio L |
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| 刘兴 等: ""黑水虻替代鱼粉对锦鲤生长和健康状况的影响"", 《大连海洋大学学报》 * |
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