CN105505992A - Method for producing functional food through probiotics compound biological fermentation - Google Patents
Method for producing functional food through probiotics compound biological fermentation Download PDFInfo
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- CN105505992A CN105505992A CN201610012379.7A CN201610012379A CN105505992A CN 105505992 A CN105505992 A CN 105505992A CN 201610012379 A CN201610012379 A CN 201610012379A CN 105505992 A CN105505992 A CN 105505992A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 94
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 82
- 238000000855 fermentation Methods 0.000 title claims abstract description 75
- 230000004151 fermentation Effects 0.000 title claims abstract description 75
- 235000013376 functional food Nutrition 0.000 title claims abstract description 44
- 150000001875 compounds Chemical class 0.000 title claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 241000894006 Bacteria Species 0.000 claims abstract description 126
- 239000007788 liquid Substances 0.000 claims abstract description 100
- 241001264174 Cordyceps militaris Species 0.000 claims abstract description 87
- 238000000034 method Methods 0.000 claims abstract description 33
- 235000015097 nutrients Nutrition 0.000 claims abstract description 29
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 27
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 27
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 14
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 14
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 14
- 230000002779 inactivation Effects 0.000 claims abstract description 6
- 230000004913 activation Effects 0.000 claims description 60
- 230000000529 probiotic effect Effects 0.000 claims description 58
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 54
- 239000000843 powder Substances 0.000 claims description 54
- 241000233866 Fungi Species 0.000 claims description 42
- 230000001954 sterilising effect Effects 0.000 claims description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 239000000047 product Substances 0.000 claims description 33
- 239000012153 distilled water Substances 0.000 claims description 30
- 230000001580 bacterial effect Effects 0.000 claims description 29
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 27
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 27
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 25
- 239000002131 composite material Substances 0.000 claims description 25
- 238000012258 culturing Methods 0.000 claims description 25
- 239000001888 Peptone Substances 0.000 claims description 24
- 108010080698 Peptones Proteins 0.000 claims description 24
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 24
- 235000019319 peptone Nutrition 0.000 claims description 24
- 239000002994 raw material Substances 0.000 claims description 21
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 20
- 229930006000 Sucrose Natural products 0.000 claims description 20
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 20
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 20
- 229920000053 polysorbate 80 Polymers 0.000 claims description 20
- 229910019142 PO4 Inorganic materials 0.000 claims description 19
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 19
- 239000010452 phosphate Substances 0.000 claims description 19
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 19
- 239000011591 potassium Substances 0.000 claims description 19
- 229910052700 potassium Inorganic materials 0.000 claims description 19
- 238000012262 fermentative production Methods 0.000 claims description 18
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 16
- 235000015278 beef Nutrition 0.000 claims description 16
- 229940041514 candida albicans extract Drugs 0.000 claims description 16
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 16
- 239000001632 sodium acetate Substances 0.000 claims description 16
- 235000017281 sodium acetate Nutrition 0.000 claims description 16
- 239000005720 sucrose Substances 0.000 claims description 16
- 239000012138 yeast extract Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 13
- 241000194017 Streptococcus Species 0.000 claims description 13
- 241001037822 Bacillus bacterium Species 0.000 claims description 12
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 12
- 240000003768 Solanum lycopersicum Species 0.000 claims description 12
- 244000068988 Glycine max Species 0.000 claims description 11
- 235000010469 Glycine max Nutrition 0.000 claims description 11
- 244000061456 Solanum tuberosum Species 0.000 claims description 11
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 11
- 238000004458 analytical method Methods 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- 244000017020 Ipomoea batatas Species 0.000 claims description 6
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 5
- 239000001527 calcium lactate Substances 0.000 claims description 5
- 229960002401 calcium lactate Drugs 0.000 claims description 5
- 235000011086 calcium lactate Nutrition 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 4
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 210000004185 liver Anatomy 0.000 claims description 4
- 210000000496 pancreas Anatomy 0.000 claims description 4
- 108010009004 proteose-peptone Proteins 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 235000015193 tomato juice Nutrition 0.000 claims description 4
- 108010046845 tryptones Proteins 0.000 claims description 4
- FITPCXSHEGAMCJ-JJKGCWMISA-N ClC(=O)[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.[Na] Chemical compound ClC(=O)[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.[Na] FITPCXSHEGAMCJ-JJKGCWMISA-N 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 235000021317 phosphate Nutrition 0.000 claims description 2
- 230000036541 health Effects 0.000 abstract description 6
- 238000009630 liquid culture Methods 0.000 abstract 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 abstract 2
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 238000001291 vacuum drying Methods 0.000 description 12
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 10
- 241000190633 Cordyceps Species 0.000 description 10
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 10
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 10
- 150000004676 glycans Chemical class 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 229920001282 polysaccharide Polymers 0.000 description 10
- 239000005017 polysaccharide Substances 0.000 description 10
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 9
- 239000011724 folic acid Substances 0.000 description 9
- 229960000304 folic acid Drugs 0.000 description 9
- 235000019152 folic acid Nutrition 0.000 description 9
- 239000004615 ingredient Substances 0.000 description 9
- 230000000050 nutritive effect Effects 0.000 description 9
- 238000005469 granulation Methods 0.000 description 6
- 230000003179 granulation Effects 0.000 description 6
- 238000005286 illumination Methods 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 4
- 235000011054 acetic acid Nutrition 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 230000009970 fire resistant effect Effects 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- -1 polypropylene Polymers 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000002775 capsule Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000010025 steaming Methods 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007937 lozenge Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
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- 210000004994 reproductive system Anatomy 0.000 description 1
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- 150000008163 sugars Chemical class 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
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- General Chemical & Material Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method for producing functional food through probiotics compound biological fermentation includes the steps that firstly, a converted cordyceps militaris culture solution is prepared and then fermented, and a cordyceps militaris fermentation product is obtained; then a strain and spores in the cordyceps militaris fermentation product are subjected to inactivation treatment; then, streptococcus thermophilus liquid culture, lactobacillus bulgaricus liquid culture, bifidobacterium liquid culture, lactobacillus acidophilus liquid culture and compound active bacterium liquid culture are carried out; then, a secondary fermentation basal culture medium is prepared, after the secondary fermentation basal culture medium is sterilized, one or more of streptococcus thermophilus liquid, lactobacillus bulgaricus liquid, bifidobacterium, lactobacillus acidophilus liquid and compound active bacterium liquid are inoculated for culture, and a probiotics secondary fermentation product is obtained; then the probiotics secondary fermentation product is dried. The functional food produced through the method contains rich nutrients, can be absorbed by human bodies easily, and promotes human body health.
Description
Technical field
The present invention relates to food processing technology field, and in particular to a kind of method of probiotic bacterium compound bio fermentative production functional food.
Background technology
Cordyceps militaris (L.) Link. (Cordycepsmilitaris) has another name called Cordyceps militaris, pupa grass etc., it is a kind of famous and precious dietotherapeutic fungi, containing various bioactivators such as cordycepin, Cordyceps polysaccharide, cordycepic acids, there is anticancer antitumor, the effect such as antisepsis and anti-inflammation, raising immunizing power.Cordyceps militaris (L.) Link. common mostly in the market is by artificial breeding technique, utilizes cereal to form for Major Nutrient substrate fermentation, this technology comparative maturity, and part Edible Fungi enterprise has started mass-producing application.Probiotic bacterium is the living microorganism that a class is useful to host, is to be colonizated in human intestinal, reproductive system, can produce definite health efficacy thus improve host's microecological balance, plays the active beneficial microorganism general name of beneficial effect.Bacterium useful in human body, animal body or fungi mainly contain: clostridium butyricum, milk-acid bacteria, bifidus bacillus, Lactobacterium acidophilum, actinomycetes, yeast etc.The composite reactive probiotic bacterium of the most powerful product of the function studied in the world at present mainly above each quasi-microorganism composition, it is widely used in biotechnology, industrial or agricultural, food safety and life and health field.But existing market lacks containing cordycepin, cordycepic acid, Cordyceps polysaccharide, the functional foodstuff simultaneously also containing lactic acid, acetic acid, folic acid, Sumylact L.
Summary of the invention
The object of the present invention is to provide a kind of method of probiotic bacterium compound bio fermentative production functional food, this functional food contains cordycepin, cordycepic acid, Cordyceps polysaccharide, simultaneously also containing lactic acid, acetic acid, folic acid, Sumylact L, there is abundant nutritive ingredient, be conducive to absorption of human body, promote health.
The present invention solves its technical problem and realizes by the following technical solutions.
A method for probiotic bacterium compound bio fermentative production functional food, comprises Cordyceps militaris (L.) Link. fungus fermented product preparation process, Cordyceps militaris (L.) Link. fungus fermented product treatment step, probiotics bacterial liquid culturing step, probiotic bacterium Secondary Fermentation step, probiotic bacterium Secondary Fermentation thing treatment step.Cordyceps militaris (L.) Link. fungus fermented product preparation process first prepares to transform Cordyceps militaris (L.) Link. nutrient solution, then ferment to conversion Cordyceps militaris (L.) Link. nutrient solution, thus obtain Cordyceps militaris (L.) Link. fungus fermented product.Cordyceps militaris (L.) Link. fungus fermented product treatment step carries out inactivation process to the bacterial classification in Cordyceps militaris (L.) Link. fungus fermented product and spore.Probiotics bacterial liquid culturing step carries out the cultivation of thermophilus streptococcus bacterium liquid, the cultivation of lactobacillus bulgaricus bacterium liquid, the cultivation of bifidus bacillus bacterium liquid, the cultivation of Lactobacterium acidophilum bacterium liquid and composite reactive bacterium liquid respectively to cultivate.Probiotic bacterium Secondary Fermentation step first prepares Secondary Fermentation basic medium, after the sterilizing of Secondary Fermentation basic medium, access one or more in thermophilus streptococcus bacterium liquid, lactobacillus bulgaricus bacterium liquid, bifidus bacillus bacterium liquid, Lactobacterium acidophilum bacterium liquid, composite reactive bacterium liquid again, cultivate, obtain probiotic bacterium Secondary Fermentation thing.Probiotic bacterium Secondary Fermentation thing treatment step is that probiotic bacterium Secondary Fermentation thing is carried out drying, obtains functional food raw material after drying.
Further, in above-mentioned Cordyceps militaris (L.) Link. fungus fermented product preparation process, preparation transforms Cordyceps militaris (L.) Link. nutrient solution is join in distilled water by potato and analysis for soybean powder, boil 15 ~ 20min, filter again, after filtrate is cooled, again white sugar, potassium primary phosphate, magnesium sulfate and calcium lactate are added in filtrate, carry out constant volume.
Further, before the described conversion Cordyceps militaris (L.) Link. nutrient solution of above-mentioned Cordyceps militaris (L.) Link. fungus liquid access, sweet potato residue, bean dregs and corn are added described conversion Cordyceps militaris (L.) Link. nutrient solution.
Further, above-mentioned Cordyceps militaris (L.) Link. fungus fermented product treatment step places 25 ~ 40min under Cordyceps militaris (L.) Link. fungus fermented product being placed in 68 ~ 72 DEG C of temperature, continues cultivation 11 ~ 13h, again at 68 ~ 72 DEG C of temperature, place 25 ~ 40min after being cooled to room temperature.
Further, in above-mentioned probiotics bacterial liquid culturing step, thermophilus streptococcus bacterium liquid is cultivated is first by weight by yeast extract paste 7 ~ 8 parts, peptone 7 ~ 8 parts, glucose 9 ~ 11 parts, potassium primary phosphate 2 ~ 3 parts, Tomato juice 95 ~ 105 parts, tween-80 0.5 ~ 1 part, distilled water 850 ~ 950 parts is placed 20 ~ 25min and is carried out sterilizing at 110 ~ 120 DEG C of temperature, all be mixed to form streptococcus thermophilus strain activation medium again, the pH value of streptococcus thermophilus strain activation medium is 6.7 ~ 6.9, then streptococcus thermophilus strain activation medium is cultivated 45 ~ 50 hours at the temperature of 41 ~ 43 DEG C.
Further, in above-mentioned probiotics bacterial liquid culturing step, the cultivation of lactobacillus bulgaricus bacterium liquid comprises is first by weight by Tryptones 9 ~ 11 parts, dipotassium hydrogen phosphate 0.4 ~ 0.6 part, yeast extract 9 ~ 11 parts, lactose 5 ~ 6 parts, sucrose 5 ~ 6 parts, 2 ~ 3 parts, calcium carbonate, purpurum bromocresolis 0.02 ~ 0.03 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 123 DEG C of temperature, wherein calcium carbonate separates sterilizing, all be mixed to form lactobacillus bulgaricus strain activation and culture base again, the pH value of lactobacillus bulgaricus strain activation and culture base is 6.3 ~ 6.5, cultivation is carried out 45 ~ 50 hours under again lactobacillus bulgaricus strain activation and culture base being placed on 40 ~ 43 DEG C of temperature.
Further, in above-mentioned probiotics bacterial liquid culturing step, bifidus bacillus bacterium liquid is cultivated is first by weight by peptone 9 ~ 10 parts, liver leaching 5 ~ 6 parts, powder, beef extract 3 ~ 4 parts, yeast extract 5 ~ 6 parts, pancreas casein peptone 7 ~ 8 parts, Zulkovsky starch 0.4 ~ 0.6 part, 1 ~ 2 part, sodium-chlor, glucose 9 ~ 11 parts, dipotassium hydrogen phosphate 1 ~ 2 part, potassium primary phosphate 1 ~ 2 part, 0.01 ~ 0.02 part, ferric sulfate, manganous sulfate 0.005 ~ 0.006 part, Cys 0.4 ~ 0.6 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form bifidobacterium species activation medium again, the pH value of bifidobacterium species activation medium is 7.1 ~ 7.3, cultivation is carried out 45 ~ 50 hours under again bifidobacterium species activation medium being placed on 36 ~ 38 DEG C of temperature.
Further, in above-mentioned probiotics bacterial liquid culturing step, Lactobacterium acidophilum bacterium liquid is cultivated is first by weight by peptone 9 ~ 10 parts, extractum carnis 9 ~ 11 parts, yeast extract paste 5 ~ 6 parts, diammonium hydrogen citrate 2 ~ 3 parts, glucose 19 ~ 21 parts, sodium acetate 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, 0.55 ~ 0.6 part, magnesium sulfate, manganous sulfate 0.2 ~ 0.3 part, tween-80 0.5 ~ 2 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form Lactobacillus acidophilus species's activation medium again, the pH value of Lactobacillus acidophilus species's activation medium is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under again Lactobacillus acidophilus species's activation medium being placed on 36 ~ 38 DEG C of temperature.
Further, in above-mentioned probiotics bacterial liquid culturing step, composite reactive bacterium liquid is cultivated is first by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, sodium acetate 5 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 1.5 ~ 2.5 parts, 0.1 ~ 0.3 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 122 DEG C of temperature, all be mixed to form composite reactive bacterium strain activation and culture base again, the pH value of composite reactive bacterium strain activation and culture base is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under again composite reactive bacterium strain activation and culture base being placed on 36 ~ 38 DEG C of temperature.
Further, preparing Secondary Fermentation basic medium in above-mentioned probiotic bacterium Secondary Fermentation step is first by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 4 ~ 6 parts, dipotassium hydrogen phosphate 1.5 ~ 2.5 parts, sodium acetate 4 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 1.5 ~ 2.5 parts, 0.1 ~ 0.3 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, again Secondary Fermentation basic medium is placed on 14 ~ 16min at 120 ~ 122 DEG C of temperature.
The beneficial effect of the method for the probiotic bacterium compound bio fermentative production functional food of the embodiment of the present invention is: this method is produced the functional food obtained and contained cordycepin, cordycepic acid, Cordyceps polysaccharide, simultaneously also containing lactic acid, acetic acid, folic acid, Sumylact L, there is abundant nutritive ingredient, be conducive to absorption of human body, promote health.In addition, the method also changes common Cordyceps militaris (L.) Link. artificial fermentation mode, alleviates the demand to grain, greatly reduces Cordyceps militaris (L.) Link. production cost.
Embodiment
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, be clearly and completely described to the technical scheme in the embodiment of the present invention below.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturers suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
Below the method for the probiotic bacterium compound bio fermentative production functional food of the embodiment of the present invention is specifically described.
A method for probiotic bacterium compound bio fermentative production functional food, comprising:
S1, Cordyceps militaris (L.) Link. fungus fermented product preparation process: preparation transforms Cordyceps militaris (L.) Link. nutrient solution, then conversion Cordyceps militaris (L.) Link. nutrient solution is fermented, obtain Cordyceps militaris (L.) Link. fungus fermented product.
1, by weight 90 ~ 110 parts, potato, analysis for soybean powder 9 ~ 11 parts are all joined steaming 750 ~ 900 parts and heat up in a steamer in water, boil 15 ~ 20min, filter again, after filtrate is cooled to room temperature, again 9 ~ 11 portions of white sugars, 0.8 ~ 1.2 part of potassium primary phosphate, 0.4 ~ 0.6 part of magnesium sulfate and 0.9 ~ 1.1 part of calcium lactate are added in filtrate, carry out constant volume, thus make conversion Cordyceps militaris (L.) Link. nutrient solution.
2, sweet potato residue 48 ~ 52 parts, 48 ~ 52 parts, bean dregs and corn 22 ~ 28 parts is put in a reservoir by weight, add again and transform Cordyceps militaris (L.) Link. nutrient solution 65 ~ 75 parts, after mixing, vessel port breathable material is sealed, at the temperature of 110 ~ 120 DEG C, place 28 ~ 35min carry out sterilizing, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use.
3, cultivation Cordyceps militaris (L.) Link. fungus liquid is carried out: add potato 190 ~ 210g, glucose 18 ~ 22g, analysis for soybean powder 9 ~ 11g, potassium primary phosphate 0.9 ~ 1.1g, magnesium sulfate 0.4 ~ 0.6g in every 1L water, after boiling, filter, add agar powder 24 ~ 26g.By the substratum packing test tube of above-mentioned formula, add tampon 115 DEG C of sterilizings 30 minutes in Autoclave, take out that to place inclined-plane for subsequent use.Aseptically expanding species, the expansion of a female kind connects 100 ± 10.Under Mother culture condition 20-25 DEG C constant temperature, cultivate 6-10 days, become ripe healthy and strong mycelia.In 1L water, add potato 190 ~ 210g again, glucose 18 ~ 22g, analysis for soybean powder 9 ~ 11g, potassium primary phosphate 1g, magnesium sulfate 0.5g, boil, and filters.Liquid nutrient medium is distributed in 500ml triangular flask, often bottled enter nutrient solution 250ml, bottleneck gas permeable material is sealed, 115 DEG C of sterilizings 30 minutes.The mother of above-mentioned cultivation maturation is planted mycelia aseptically with in transfering loop access triangular flask substratum, 1 test tube slant bacterial classification connects 40 ~ 50 bottles.The triangular flask having connect bacterium is cultivated on shaking table, temperature 18 ~ 25 DEG C, rotating speed 110 ~ 150r/min, and cultivate after 3 ~ 6 days, mycelial growth is vigorous, namely can be used as Cordyceps militaris (L.) Link. fungus liquid.
4, by weight with in Cordyceps militaris (L.) Link. nutrient solution for subsequent use after sterilizing in the container in spraying vaccinization rifle inoculation 12 ~ 13 parts of Cordyceps militaris (L.) Link. fungus liquid to 2 of step S1, finished container moves on the culturing rack of the culturing room of adjustable temperature control humidity and illumination, in temperature 18 ~ 25 DEG C, under the condition of humidity 50% ~ 70%, lucifuge is cultivated, cover with after substratum until white hypha, regulate needed for humiture and illumination extremely, after 2 ~ 5 days, white hypha turns yellow, results Cordyceps militaris (L.) Link. fermented product.Utilize sweet potato residue, bean dregs, corn to carry out Cordyceps militaris (L.) Link. fermentation for matrix, processing byproduct can be converted into the high added value raw material or product with nourishing function, greatly reduce production cost, alleviate grain demand.
S2, Cordyceps militaris (L.) Link. fungus fermented product treatment step: inactivation process is carried out to the bacterial classification in Cordyceps militaris (L.) Link. fungus fermented product and spore.
Place 25 ~ 40min under first Cordyceps militaris (L.) Link. fungus fermented product being placed in 68 ~ 72 DEG C of temperature, after being cooled to room temperature, continue cultivation 11 ~ 13h, again at 68 ~ 72 DEG C of temperature, place 25 ~ 40min.
S3, probiotics bacterial liquid culturing step: carry out the cultivation of thermophilus streptococcus bacterium liquid, the cultivation of lactobacillus bulgaricus bacterium liquid respectively, bifidus bacillus bacterium liquid is cultivated, Lactobacterium acidophilum bacterium liquid is cultivated, composite reactive bacterium liquid is cultivated.
1, thermophilus streptococcus bacterium liquid is cultivated: first by weight yeast extract paste 7 ~ 8 parts, peptone 7 ~ 8 parts, glucose 9 ~ 11 parts, potassium primary phosphate 2 ~ 3 parts, Tomato juice 95 ~ 105 parts, tween-80 0.5 ~ 1 part, distilled water 850 ~ 950 parts are placed at 110 ~ 120 DEG C of temperature 20 ~ 25min and carry out sterilizing, all be mixed to form streptococcus thermophilus strain activation medium again, the pH value of streptococcus thermophilus strain activation medium is 6.7 ~ 6.9, is then cultivated 45 ~ 50 hours at the temperature of 41 ~ 43 DEG C by streptococcus thermophilus strain activation medium.
2, lactobacillus bulgaricus bacterium liquid is cultivated: first by weight by Tryptones 9 ~ 11 parts, dipotassium hydrogen phosphate 0.4 ~ 0.6 part, yeast extract 9 ~ 11 parts, lactose 5 ~ 6 parts, sucrose 5 ~ 6 parts, 2 ~ 3 parts, calcium carbonate, purpurum bromocresolis 0.02 ~ 0.03 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 123 DEG C of temperature, wherein calcium carbonate separates sterilizing, all be mixed to form lactobacillus bulgaricus strain activation and culture base again, the pH value of lactobacillus bulgaricus strain activation and culture base is 6.3 ~ 6.5, cultivation is carried out 45 ~ 50 hours under again lactobacillus bulgaricus strain activation and culture base being placed on 40 ~ 43 DEG C of temperature.
3, bifidus bacillus bacterium liquid is cultivated: first by weight by peptone 9 ~ 10 parts, liver leaching 5 ~ 6 parts, powder, beef extract 3 ~ 4 parts, yeast extract 5 ~ 6 parts, pancreas casein peptone 7 ~ 8 parts, Zulkovsky starch 0.4 ~ 0.6 part, 1 ~ 2 part, sodium-chlor, glucose 9 ~ 11 parts, dipotassium hydrogen phosphate 1 ~ 2 part, potassium primary phosphate 1 ~ 2 part, 0.01 ~ 0.02 part, ferric sulfate, manganous sulfate 0.005 ~ 0.006 part, Cys 0.4 ~ 0.6 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form bifidobacterium species activation medium again, the pH value of bifidobacterium species activation medium is 7.1 ~ 7.3, cultivation is carried out 45 ~ 50 hours under again bifidobacterium species activation medium being placed on 36 ~ 38 DEG C of temperature.
4, Lactobacterium acidophilum bacterium liquid is cultivated: first by weight by peptone 9 ~ 10 parts, extractum carnis 9 ~ 11 parts, yeast extract paste 5 ~ 6 parts, diammonium hydrogen citrate 2 ~ 3 parts, glucose 19 ~ 21 parts, sodium acetate 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, 0.55 ~ 0.6 part, magnesium sulfate, manganous sulfate 0.2 ~ 0.3 part, tween-80 0.5 ~ 2 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form Lactobacillus acidophilus species's activation medium again, the pH value of Lactobacillus acidophilus species's activation medium is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under again Lactobacillus acidophilus species's activation medium being placed on 36 ~ 38 DEG C of temperature.
5, composite reactive bacterium liquid is cultivated: first by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, sodium acetate 5 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 1.5 ~ 2.5 parts, 0.1 ~ 0.3 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 122 DEG C of temperature, all be mixed to form composite reactive bacterium strain activation and culture base again, the pH value of composite reactive bacterium strain activation and culture base is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under again composite reactive bacterium strain activation and culture base being placed on 36 ~ 38 DEG C of temperature.
S4, probiotic bacterium Secondary Fermentation step: prepare Secondary Fermentation basic medium, after the sterilizing of Secondary Fermentation basic medium, access one or more in thermophilus streptococcus bacterium liquid, lactobacillus bulgaricus bacterium liquid, bifidus bacillus bacterium liquid, Lactobacterium acidophilum bacterium liquid, composite reactive bacterium liquid, cultivate, obtain probiotic bacterium Secondary Fermentation thing.
First, by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 4 ~ 6 parts, dipotassium hydrogen phosphate 1.5 ~ 2.5 parts, sodium acetate 4 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 15 ~ 25 parts, 0.1 ~ 03 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, again Secondary Fermentation basic medium is placed on 14 ~ 16min at 120 ~ 122 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use.
Afterwards, access one or more in thermophilus streptococcus bacterium liquid, lactobacillus bulgaricus bacterium liquid, bifidus bacillus bacterium liquid, Lactobacterium acidophilum bacterium liquid, composite reactive bacterium liquid again, the volume of the probiotics bacterial liquid of access is 14 ~ 16mL, cultivate 48 ~ 50 hours at 37 ~ 42 DEG C, obtain probiotic bacterium Secondary Fermentation thing.
S5, probiotic bacterium Secondary Fermentation thing treatment step: probiotic bacterium Secondary Fermentation thing is dry, obtain functional food raw material.
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
Further, also comprise forming process step after step S5, forming process step is that functional food Raw material processing is become lozenge, capsule or pulvis.
Below in conjunction with embodiment, characteristic sum performance of the present invention is described in further detail.
Embodiment 1
The first step, Cordyceps militaris (L.) Link. fungus fermented product preparation process:
1, by weight potato 100g, analysis for soybean powder 10g being joined steaming 800mL heats up in a steamer in water, boil 15min, filter with gauze again, after filtrate is cooled to room temperature, again 10g white sugar, 1.0g potassium primary phosphate, 0.5g magnesium sulfate and 1.0g calcium lactate are added in filtrate, be settled to 1000mL, obtain transforming Cordyceps militaris (L.) Link. nutrient solution.
2, in Cans, put into sweet potato residue 50g, bean dregs 50g and corn 25g, add and transform Cordyceps militaris (L.) Link. nutrient solution 70mL, after mixing, bottleneck fire resistant polypropylene film is sealed, at the temperature of 115 DEG C, place 30min carry out sterilizing, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use.
3, cultivation Cordyceps militaris (L.) Link. fungus liquid is carried out: every 1L water adds potato 200g, glucose 20g, analysis for soybean powder 10g, potassium primary phosphate 1.0g, magnesium sulfate 0.5g, after boiling, filters, and adds agar powder 25.By the substratum packing test tube of above-mentioned formula, add tampon 115 DEG C of sterilizings 30 minutes in Autoclave, take out that to place inclined-plane for subsequent use.Aseptically expanding species, the expansion of a female kind connects 100 ± 10.Under Mother culture condition 20 ~ 25 DEG C of constant temperature, cultivate 6 ~ 10 days, become ripe healthy and strong mycelia.In 1L water, add potato 200g again, glucose 20g, analysis for soybean powder 10g, potassium primary phosphate 1g, magnesium sulfate 0.5g, boil, and filters.Liquid nutrient medium is distributed in 500ml triangular flask, often bottled enter nutrient solution 250ml, the ventilative fire resistant polypropylene film of bottleneck is sealed, 115 DEG C of sterilizings 30 minutes.The mother of above-mentioned cultivation maturation is planted mycelia aseptically with in transfering loop access triangular flask substratum, 1 test tube slant bacterial classification connects 45 bottles.The triangular flask having connect bacterium is cultivated on shaking table, temperature 19 DEG C, rotating speed 130r/min, and cultivate after 3 ~ 6 days, mycelial growth is vigorous, namely can be used as Cordyceps militaris (L.) Link. fungus liquid.
4, by weight with in Cordyceps militaris (L.) Link. nutrient solution for subsequent use after sterilizing in the container in spraying vaccinization rifle inoculation 12.5mL Cordyceps militaris (L.) Link. fungus liquid to 2 of step S1, finished container moves on the culturing rack of the culturing room of adjustable temperature control humidity and illumination, in temperature 18 ~ 25 DEG C, under the condition of humidity 50% ~ 70%, lucifuge is cultivated, cover with after substratum until white hypha, regulate needed for humiture and illumination extremely, after 2 ~ 5 days, white hypha turns yellow, results Cordyceps militaris (L.) Link. fermented product.
Second step, Cordyceps militaris (L.) Link. fungus fermented product treatment step:
Place 30min under first Cordyceps militaris (L.) Link. fungus fermented product being placed in 70 DEG C of temperature, continue to cultivate 12h after being cooled to room temperature, again at 70 DEG C of temperature, place 30min, make bacterial classification wherein and spore inactivation.
3rd step, probiotics bacterial liquid culturing step:
Thermophilus streptococcus bacterium liquid is cultivated: first by weight yeast extract paste 7.5g, peptone 7.5g, glucose 10g, potassium primary phosphate 2g, Tomato juice 100mL, tween-80 0.5mL, distilled water 900mL are placed 20min at 115 DEG C and carry out sterilizing, all be mixed to form streptococcus thermophilus strain activation medium again, the pH value of streptococcus thermophilus strain activation medium is 6.7 ~ 6.9, is then cultivated 48 hours at the temperature of 42 DEG C by streptococcus thermophilus strain activation medium.
4th step, probiotic bacterium Secondary Fermentation step:
First, by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g, manganous sulfate 0.04g, distilled water 1000mL are mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, Secondary Fermentation basic medium is placed on 15min at 121 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use again.
Afterwards, then access 15mL thermophilus streptococcus bacterium liquid, cultivate 48 hours at 42 DEG C, obtain probiotic bacterium Secondary Fermentation thing;
5th step, probiotic bacterium Secondary Fermentation thing treatment step:
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
6th step, forming process step, becomes lozenge by functional food Raw material processing.
Wherein, the nutritive ingredient of gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material is: cordycepin 0.43%, Cordyceps polysaccharide 5.71%, Pfansteihl 76g/kg, folic acid 42ng/g.
Embodiment 2
The first step, Cordyceps militaris (L.) Link. fungus fermented product preparation process:
1, by weight potato 95g, analysis for soybean powder 9g being joined steaming 750mL heats up in a steamer in water, boil 17min, filter with gauze again, after filtrate is cooled to room temperature, again 10g white sugar, 1.0g potassium primary phosphate, 0.4g magnesium sulfate and 1.0g calcium lactate are added in filtrate, be settled to 1000mL, obtain transforming Cordyceps militaris (L.) Link. nutrient solution.
2, in Cans, put into sweet potato residue 52g, bean dregs 52g and corn 28g, add and transform Cordyceps militaris (L.) Link. nutrient solution 73mL, after mixing, bottleneck fire resistant polypropylene film is sealed, at the temperature of 116 DEG C, place 30min carry out sterilizing, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use.
3, cultivation Cordyceps militaris (L.) Link. fungus liquid is carried out: add potato 210g, glucose 21g, analysis for soybean powder 11g, potassium primary phosphate 1.1g, magnesium sulfate 0.5g in every 1L water, after boiling, filter, add agar powder 25.By the substratum packing test tube of above-mentioned formula, add tampon 115 DEG C of sterilizings 30 minutes in Autoclave, take out that to place inclined-plane for subsequent use.Aseptically expanding species, the expansion of a female kind connects 100 ± 10.Under Mother culture condition 20 ~ 25 DEG C of constant temperature, cultivate 6 ~ 10 days, become ripe healthy and strong mycelia.In 1L water, add potato 210g, glucose 21g, analysis for soybean powder 11g, potassium primary phosphate 1.1g, magnesium sulfate 0.5g again, boil, filter.Liquid nutrient medium is distributed in 500ml triangular flask, often bottled enter nutrient solution 250ml, the ventilative fire resistant polypropylene film of bottleneck is sealed, 115 DEG C of sterilizings 30 minutes.The mother of above-mentioned cultivation maturation is planted mycelia aseptically with in transfering loop access triangular flask substratum, 1 test tube slant bacterial classification connects 50 bottles.The triangular flask having connect bacterium is cultivated on shaking table, temperature 22 DEG C, rotating speed 130r/min, and cultivate after 3 ~ 6 days, mycelial growth is vigorous, namely can be used as Cordyceps militaris (L.) Link. fungus liquid.
4, by weight with in Cordyceps militaris (L.) Link. nutrient solution for subsequent use after sterilizing in the container in spraying vaccinization rifle inoculation 12.8mL Cordyceps militaris (L.) Link. fungus liquid to 2 of step S1, finished container moves on the culturing rack of the culturing room of adjustable temperature control humidity and illumination, in temperature 18 ~ 25 DEG C, under the condition of humidity 50% ~ 70%, lucifuge is cultivated, cover with after substratum until white hypha, regulate needed for humiture and illumination extremely, after 2 ~ 5 days, white hypha turns yellow, results Cordyceps militaris (L.) Link. fermented product.
Second step, Cordyceps militaris (L.) Link. fungus fermented product treatment step:
Place 35min under first Cordyceps militaris (L.) Link. fungus fermented product being placed in 71 DEG C of temperature, continue to cultivate 12h after being cooled to room temperature, again at 71 DEG C of temperature, place 35min, make bacterial classification wherein and spore inactivation.
3rd step, probiotics bacterial liquid culturing step:
Lactobacillus bulgaricus bacterium liquid is cultivated: first by weight Tryptones 10g, dipotassium hydrogen phosphate 0.5g, yeast extract 10g, lactose 5g, sucrose 5g, calcium carbonate 2g, purpurum bromocresolis 0.02g, distilled water 1000mL are placed 20min at 121 DEG C of temperature and carry out sterilizing, wherein calcium carbonate separates sterilizing, all be mixed to form lactobacillus bulgaricus strain activation and culture base again, the pH value of lactobacillus bulgaricus strain activation and culture base is 6.3 ~ 6.5, then carries out cultivation 48 hours under lactobacillus bulgaricus strain activation and culture base is placed on 42 DEG C of temperature.
4th step, probiotic bacterium Secondary Fermentation step:
First, by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g, manganous sulfate 0.04g, distilled water 1000mL are mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, Secondary Fermentation basic medium is placed on 15min at 121 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use again.
Afterwards, then access 15mL lactobacillus bulgaricus bacterium liquid, cultivate 48 hours at 42 DEG C, obtain probiotic bacterium Secondary Fermentation thing;
5th step, probiotic bacterium Secondary Fermentation thing treatment step:
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
6th step, forming process step, becomes pulvis by functional food Raw material processing.
Wherein, the nutritive ingredient of gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material is: cordycepin 0.41%, Cordyceps polysaccharide 5.81%, Pfansteihl 102g/kg, folic acid 58ng/g.
Embodiment 3
The present embodiment difference from Example 1 is:
3rd step, probiotics bacterial liquid culturing step:
Bifidus bacillus bacterium liquid is cultivated: first by weight by peptone 10g, liver leaching powder 5g, beef extract 3g, yeast extract 5g, pancreas casein peptone 8g part, Zulkovsky starch 0.5g part, sodium-chlor 1.5g, glucose 10g, dipotassium hydrogen phosphate 1.5g, potassium primary phosphate 1g, ferric sulfate 0.02g, manganous sulfate 0.005g, Cys 0.5g, distilled water 1000mL places 20min and carries out sterilizing at 115 DEG C of temperature, all be mixed to form bifidobacterium species activation medium again, the pH value of bifidobacterium species activation medium is 7.1 ~ 7.3, cultivation is carried out 48 hours under again bifidobacterium species activation medium being placed on 37 DEG C of temperature.
4th step, probiotic bacterium Secondary Fermentation step:
First, by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g, manganous sulfate 0.04g, distilled water 1000mL are mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, Secondary Fermentation basic medium is placed on 15min at 121 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use again.
Afterwards, then access 15mL bifidus bacillus bacterium liquid, cultivate 48 hours at 42 DEG C, obtain probiotic bacterium Secondary Fermentation thing;
5th step, probiotic bacterium Secondary Fermentation thing treatment step:
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
6th step, forming process step, becomes pulvis by functional food Raw material processing.
Wherein, the nutritive ingredient of gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material is: cordycepin 0.39%, Cordyceps polysaccharide 5.57%, Pfansteihl 89g/kg, folic acid 12ng/g.
Embodiment 4
The present embodiment difference from Example 2 is:
3rd step, probiotics bacterial liquid culturing step:
Lactobacterium acidophilum bacterium liquid is cultivated: first by weight peptone 10g, extractum carnis 10g, yeast extract paste 5g, diammonium hydrogen citrate 3g, glucose 20g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, 0.58 part, magnesium sulfate, manganous sulfate 0.25 part, tween-80 1mL, distilled water 1000mL are placed 20min and carry out sterilizing at 115 DEG C of temperature, all be mixed to form Lactobacillus acidophilus species's activation medium again, the pH value of Lactobacillus acidophilus species's activation medium is 7.0 ~ 7.2, then carries out cultivation 48 hours under Lactobacillus acidophilus species's activation medium being placed on 37 DEG C of temperature.
4th step, probiotic bacterium Secondary Fermentation step:
First, by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g, manganous sulfate 0.04g, distilled water 1000mL are mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, Secondary Fermentation basic medium is placed on 15min at 121 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use again.
Afterwards, then access 15mL Lactobacterium acidophilum bacterium liquid, cultivate 48 hours at 42 DEG C, obtain probiotic bacterium Secondary Fermentation thing;
5th step, probiotic bacterium Secondary Fermentation thing treatment step:
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
6th step, forming process step, becomes capsule by functional food Raw material processing.
Wherein, the nutritive ingredient of gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material is: cordycepin 0.41%, Cordyceps polysaccharide 5.76%, Pfansteihl 123g/kg, folic acid 35ng/g.
Embodiment 5
The present embodiment difference from Example 1 is:
3rd step, probiotics bacterial liquid culturing step:
Composite reactive bacterium liquid is cultivated: first by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g part, manganous sulfate 0.04g, distilled water 1000mL places 20min and carries out sterilizing at 121 DEG C of temperature, all be mixed to form composite reactive bacterium strain activation and culture base again, the pH value of composite reactive bacterium strain activation and culture base is 7.0 ~ 7.2, cultivation is carried out 48 hours under again composite reactive bacterium strain activation and culture base being placed on 37 DEG C of temperature.
4th step, probiotic bacterium Secondary Fermentation step:
First, by weight by tomato powder 2.5g, beef powder 10g, yeast powder 5g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, tween-80 1mL, sucrose 20g, peptone 10g, diammonium hydrogen citrate 2g, magnesium sulfate 0.2g, manganous sulfate 0.04g, distilled water 1000mL are mixed to form Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, Secondary Fermentation basic medium is placed on 15min at 121 DEG C of temperature, putting into aseptic operating platform after taking-up, to be cooled to room temperature for subsequent use again.
Afterwards, access 15mL composite reactive bacterium liquid (wherein, comprising thermophilus streptococcus, lactobacillus bulgaricus, bifidus bacillus, these four kinds of bacterium liquid of Lactobacterium acidophilum, bacterial classification ratio 1:1:1:1) again, cultivate 48 hours at 37 DEG C, obtain probiotic bacterium Secondary Fermentation thing;
5th step, probiotic bacterium Secondary Fermentation thing treatment step:
Probiotic bacterium Secondary Fermentation thing is carried out low-temperature vacuum drying, low-temperature vacuum drying temperature 40 DEG C, pressure 0.3MPa, after dry, granulation obtains Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material.
6th step, forming process step, becomes capsule by functional food Raw material processing.
Wherein, the nutritive ingredient of gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment functional food raw material is: cordycepin 0.42%, Cordyceps polysaccharide 5.83%, Pfansteihl 146g/kg, folic acid 63ng/g.
Table 1 is the list of above-mentioned 5 embodiment Secondary Fermentation gained Cordyceps militaris (L.) Link.-probiotic bacterium complex ferment thing nutritive ingredient.
Table 1
In sum, the method of the probiotic bacterium compound bio fermentative production functional food of the embodiment of the present invention is produced the functional food obtained and is contained cordycepin, cordycepic acid, Cordyceps polysaccharide, simultaneously also containing lactic acid, acetic acid, folic acid, Sumylact L, there is abundant nutritive ingredient, be conducive to absorption of human body, promote health.In addition, the method also changes common Cordyceps militaris (L.) Link. artificial fermentation mode, alleviates the demand to grain, greatly reduces Cordyceps militaris (L.) Link. production cost.
Embodiment described above is the present invention's part embodiment, instead of whole embodiments.The detailed description of embodiments of the invention the claimed scope of the present invention of not intended to be limiting, but only represent selected embodiment of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Claims (10)
1. a method for probiotic bacterium compound bio fermentative production functional food, is characterized in that, comprising:
Cordyceps militaris (L.) Link. fungus fermented product preparation process: preparation transforms Cordyceps militaris (L.) Link. nutrient solution, the more described conversion Cordyceps militaris (L.) Link. nutrient solution of Cordyceps militaris (L.) Link. fungus liquid access is fermented, obtain Cordyceps militaris (L.) Link. fungus fermented product;
Cordyceps militaris (L.) Link. fungus fermented product treatment step: inactivation process is carried out to the bacterial classification in described Cordyceps militaris (L.) Link. fungus fermented product and spore;
Probiotics bacterial liquid culturing step: carry out the cultivation of thermophilus streptococcus bacterium liquid, the cultivation of lactobacillus bulgaricus bacterium liquid respectively, bifidus bacillus bacterium liquid is cultivated, Lactobacterium acidophilum bacterium liquid is cultivated, composite reactive bacterium liquid is cultivated;
Probiotic bacterium Secondary Fermentation step: prepare Secondary Fermentation basic medium, after the sterilizing of described Secondary Fermentation basic medium, access one or more in described thermophilus streptococcus bacterium liquid, described lactobacillus bulgaricus bacterium liquid, described bifidus bacillus bacterium liquid, described Lactobacterium acidophilum bacterium liquid, described composite reactive bacterium liquid, cultivate, obtain probiotic bacterium Secondary Fermentation thing;
Probiotic bacterium Secondary Fermentation thing treatment step: described probiotic bacterium Secondary Fermentation thing is dry, obtains functional food raw material.
2. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, in described Cordyceps militaris (L.) Link. fungus fermented product preparation process, prepare described conversion Cordyceps militaris (L.) Link. nutrient solution is join in distilled water by potato and analysis for soybean powder, boil 15 ~ 20min, filter again, after filtrate is cooled, then white sugar, potassium primary phosphate, magnesium sulfate and calcium lactate are added in described filtrate, carry out constant volume.
3. the method for probiotic bacterium compound bio fermentative production functional food according to claim 2, is characterized in that, before the described conversion Cordyceps militaris (L.) Link. nutrient solution of described Cordyceps militaris (L.) Link. fungus liquid access, sweet potato residue, bean dregs and corn is added described conversion Cordyceps militaris (L.) Link. nutrient solution.
4. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, described Cordyceps militaris (L.) Link. fungus fermented product treatment step comprises: place 25 ~ 40min under described Cordyceps militaris (L.) Link. fungus fermented product being placed in 68 ~ 72 DEG C of temperature, continue cultivation 11 ~ 13h after being cooled to room temperature, again at 68 ~ 72 DEG C of temperature, place 25 ~ 40min.
5. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, thermophilus streptococcus bacterium liquid described in described probiotics bacterial liquid culturing step is cultivated and is comprised: by weight by yeast extract paste 7 ~ 8 parts, peptone 7 ~ 8 parts, glucose 9 ~ 11 parts, potassium primary phosphate 2 ~ 3 parts, Tomato juice 95 ~ 105 parts, tween-80 0.5 ~ 1 part, distilled water 850 ~ 950 parts is placed 20 ~ 25min and is carried out sterilizing at 110 ~ 120 DEG C of temperature, all be mixed to form streptococcus thermophilus strain activation medium again, the pH value of described streptococcus thermophilus strain activation medium is 6.7 ~ 6.9, then described streptococcus thermophilus strain activation medium is cultivated 45 ~ 50 hours at the temperature of 41 ~ 43 DEG C.
6. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, lactobacillus bulgaricus bacterium liquid described in described probiotics bacterial liquid culturing step is cultivated and is comprised: by weight by Tryptones 9 ~ 11 parts, dipotassium hydrogen phosphate 0.4 ~ 0.6 part, yeast extract 9 ~ 11 parts, lactose 5 ~ 6 parts, sucrose 5 ~ 6 parts, 2 ~ 3 parts, calcium carbonate, purpurum bromocresolis 0.02 ~ 0.03 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 123 DEG C of temperature, wherein said calcium carbonate separates sterilizing, all be mixed to form lactobacillus bulgaricus strain activation and culture base again, the pH value of described lactobacillus bulgaricus strain activation and culture base is 6.3 ~ 6.5, cultivation is carried out 45 ~ 50 hours under more described lactobacillus bulgaricus strain activation and culture base being placed on 40 ~ 43 DEG C of DEG C of temperature.
7. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, bifidus bacillus bacterium liquid described in described probiotics bacterial liquid culturing step is cultivated and is comprised: by weight by peptone 9 ~ 10 parts, liver leaching 5 ~ 6 parts, powder, beef extract 3 ~ 4 parts, yeast extract 5 ~ 6 parts, pancreas casein peptone 7 ~ 8 parts, Zulkovsky starch 0.4 ~ 0.6 part, 1 ~ 2 part, sodium-chlor, glucose 9 ~ 11 parts, dipotassium hydrogen phosphate 1 ~ 2 part, potassium primary phosphate 1 ~ 2 part, 0.01 ~ 0.02 part, ferric sulfate, manganous sulfate 0.005 ~ 0.006 part, Cys 0.4 ~ 0.6 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form bifidobacterium species activation medium again, the pH value of described bifidobacterium species activation medium is 7.1 ~ 7.3, cultivation is carried out 45 ~ 50 hours under more described bifidobacterium species activation medium being placed on 36 ~ 38 DEG C of temperature.
8. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, Lactobacterium acidophilum bacterium liquid described in described probiotics bacterial liquid culturing step is cultivated and is comprised: by weight by peptone 9 ~ 10 parts, extractum carnis 9 ~ 11 parts, yeast extract paste 5 ~ 6 parts, diammonium hydrogen citrate 2 ~ 3 parts, glucose 19 ~ 21 parts, sodium acetate 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, 0.55 ~ 0.6 part, magnesium sulfate, manganous sulfate 0.2 ~ 0.3 part, tween-80 0.5 ~ 2 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 114 ~ 116 DEG C of temperature, all be mixed to form Lactobacillus acidophilus species's activation medium again, the pH value of described Lactobacillus acidophilus species's activation medium is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under more described Lactobacillus acidophilus species's activation medium being placed on 36 ~ 38 DEG C of temperature.
9. the method for probiotic bacterium compound bio fermentative production functional food according to claim 1, it is characterized in that, composite reactive bacterium liquid described in described probiotics bacterial liquid culturing step is cultivated and is comprised: by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 5 ~ 6 parts, dipotassium hydrogen phosphate 2 ~ 3 parts, sodium acetate 5 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 1.5 ~ 2.5 parts, 0.1 ~ 0.3 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is placed 20 ~ 25min and is carried out sterilizing at 119 ~ 122 DEG C of temperature, all be mixed to form composite reactive bacterium strain activation and culture base again, the pH value of described composite reactive bacterium strain activation and culture base is 7.0 ~ 7.2, cultivation is carried out 45 ~ 50 hours under more described composite reactive bacterium strain activation and culture base being placed on 36 ~ 38 DEG C of temperature.
10. the method for probiotic bacterium compound bio fermentative production functional food according to claim 8, it is characterized in that, prepare described Secondary Fermentation basic medium in described probiotic bacterium Secondary Fermentation step to comprise: by weight by tomato powder 2 ~ 3 parts, beef powder 9 ~ 11 parts, yeast powder 4 ~ 6 parts, dipotassium hydrogen phosphate 1.5 ~ 2.5 parts, sodium acetate 4 ~ 6 parts, tween-80 0.5 ~ 2 part, sucrose 19 ~ 21 parts, peptone 9 ~ 11 parts, diammonium hydrogen citrate 1.5 ~ 2.5 parts, 0.1 ~ 0.3 part, magnesium sulfate, manganous sulfate 0.03 ~ 0.05 part, distilled water 900 ~ 1100 parts is mixed to form described Secondary Fermentation basic medium and pH value is 7.0 ~ 7.2, again described Secondary Fermentation basic medium is placed on 14 ~ 16min at 120 ~ 122 DEG C of temperature.
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| CN106235030A (en) * | 2016-08-17 | 2016-12-21 | 许文静 | A kind of Folium Ilicis fat reducing fermentation dried mutton and preparation method thereof |
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| CN107960470A (en) * | 2017-11-30 | 2018-04-27 | 南阳理工学院 | A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage |
| CN113710102A (en) * | 2019-04-03 | 2021-11-26 | 科郦意大利有限公司 | Process for increasing the bioavailability of saccharides from natural complex polysaccharides for human, animal and agricultural purposes |
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| CN110235822A (en) * | 2019-07-10 | 2019-09-17 | 湖北省农业科学院农产品加工与核农技术研究所 | Living aquatic product Antistress agent and its production method and application method |
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