CN110051596B

CN110051596B - Antibacterial and anti-inflammatory dendrobium toothpaste

Info

Publication number: CN110051596B
Application number: CN201910332276.2A
Authority: CN
Inventors: 周劭; 周建安; 钟捷
Original assignee: Wuhan University of Science and Engineering WUSE
Current assignee: Wuhan University of Science and Engineering WUSE
Priority date: 2019-04-24
Filing date: 2019-04-24
Publication date: 2022-05-17
Anticipated expiration: 2039-04-24
Also published as: CN110051596A

Abstract

The invention discloses an antibacterial and anti-inflammatory dendrobium toothpaste which comprises the following components in parts by weight: 2-20 parts of dendrobium extract, 5-15 parts of wetting agent, 5-10 parts of friction agent, 0.5-0.8 part of potassium sorbate, 0.5-1.0 part of thickening agent, 2-5 parts of sodium dodecyl sulfate, 0.2-0.5 part of sweetening agent, 5-15 parts of nano hydroxyapatite and 15-25 parts of deionized water; the method has the advantages of low cost of raw materials, simple and scientific formula, high extraction efficiency compared with the method of singly using the semi-bionic method or the enzyme method, suitability for industrial large-scale production and better application value, and simultaneously combines the semi-bionic method and the biological enzyme to extract active ingredients in the dendrobium, simulates the absorption and transportation process of the dendrobium in the gastrointestinal tract after oral administration.

Description

Antibacterial and anti-inflammatory dendrobium toothpaste

Technical Field

The invention relates to the field of daily necessities, in particular to antibacterial and anti-inflammatory dendrobium toothpaste.

Background

The main pathological changes of the chronic atrophic gastritis are atrophy, thinning and inflammation of mucous gland glands, which can be accompanied with intestinal glandular metaplasia and atypical hyperplasia, the incidence rate accounts for 10-20% of all chronic gastritis, and the world health organization lists the chronic atrophic gastritis as a precancerous state of stomach.

The toothpaste is an indispensable oral cavity cleaning sanitary product in daily life, the requirement of consumers on the toothpaste is higher and higher along with the enhancement of health care consciousness of people, the functional toothpaste added with the traditional Chinese medicine active ingredients becomes a mainstream development direction of the market, but the efficacy of the existing toothpaste taking the traditional Chinese medicine as the active ingredients is not obvious.

Dendrobe is a rare traditional Chinese medicinal material, and is recorded in the 'main Shang' term of Shen nong Ben Cao Jing; removing arthralgia and descending qi; tonify the five internal organs, strengthen yin, nourish yin, clear heat and promote the production of body fluid after long-term use for thick intestines and stomach; the dendrobium is rich in components such as polysaccharide, flavone, alkaloid, free amino acid, vitamin, various enzymes and the like; researches find that the dendrobium polysaccharide has the effects of resisting oxidation, resisting tumors, resisting inflammation, enhancing immunity and the like; the research on the dendrobium candidum crystal serving as the main drug discovers that the dendrobium candidum crystal can promote rats to secrete gastric juice and increase the discharge amount of gastric acid and pepsin; the external application can stop bleeding and promote wound healing; the active ingredients of the dendrobium are effectively combined with the toothpaste, so that the prevention, nursing and health care of oral diseases can be realized, the chronic atrophic gastritis is prevented, and the incidence rate of gastric cancer is reduced.

The existing preparation method of the dendrobium extract is mostly prepared by water extraction or alcohol extraction, organic solvent extraction, ultrasonic extraction, supercritical extraction and other methods; the method of water extraction or alcohol extraction is adopted, the extraction time is long, the reagent is consumed, the biologically active ingredients in the dendrobium cannot be completely extracted, and the efficacy of the dendrobium cannot be fully embodied; the ultrasonic method is adopted for extraction, has the defects of ultrasonic attenuation, large noise, strong radiation and the like, and is not suitable for industrial production; the dendrobium extract prepared by the supercritical extraction method can not extract chemical components with large molecular weight and large polarity, has low extraction rate and overhigh production cost.

The dissolution of the effective components of the traditional Chinese medicinal materials is closely related to the crushing degree of the traditional Chinese medicinal materials, the dissolution of the effective components can be improved along with the increase of the wall breaking rate, the utilization value of the traditional Chinese medicinal materials is improved, and the quality of products is improved; the dendrobium is added into the toothpaste, so that the effects of disease prevention, nursing and health care are expected to be improved; however, how to exert the efficacy and deep processing application of dendrobium to the maximum extent becomes a difficult problem which people focus on research in recent years; at present, the dendrobium powder is prepared by a common dendrobium adding mode and is added into the medicament, but the dendrobium powder is prepared by a common mode, so that the cell wall breaking rate is low, the overall utilization rate of medicinal materials is low, the release rate in a human body and a traditional Chinese medicine preparation is low, and the exertion of the medicinal effect is further influenced.

Disclosure of Invention

The invention aims to solve the problems in the prior art, and provides the antibacterial and anti-inflammatory dendrobium toothpaste which has good antibacterial and anti-inflammatory effects, has good curative effects on gingival inflammation, bleeding and oral ulcer, and can improve the disease prevention of chronic atrophic gastritis after long-term use.

In order to achieve the purpose, the invention adopts the technical scheme that:

the antibacterial and anti-inflammatory dendrobium toothpaste comprises the following components in parts by weight: 2-20 parts of dendrobium extract, 5-15 parts of wetting agent, 5-10 parts of friction agent, 0.5-0.8 part of potassium sorbate, 0.5-1.0 part of thickening agent, 2-5 parts of sodium dodecyl sulfate, 0.2-0.5 part of sweetening agent, 5-15 parts of nano-hydroxyapatite and 15-25 parts of deionized water.

As a further limitation of the above scheme, the method for extracting the dendrobium extract comprises the following steps:

s1, cleaning fresh dendrobium, and then putting the dendrobium into a low-temperature drying oven for freeze drying;

s2, pouring the frozen dendrobium into a crusher for crushing;

s3, taking the crushed dendrobium powder, extracting with simulated gastric juice, and centrifuging the extracting solution;

s4, adding disodium hydrogen phosphate-citric acid buffer solution into the filter residue obtained in the step S3, adjusting the pH value of the system to 3.0-5.0, adding enzyme for destroying plant cell walls, and performing oscillation extraction;

s5, dissolving the filter residue obtained in the step S4 in simulated intestinal juice for extraction, and centrifuging the extracting solution;

s6, merging the supernatant liquid obtained after centrifugation in the steps S3-S5, and concentrating under reduced pressure to 1/3-1/5 of the volume of the stock solution;

s7, combining the medicine residues obtained in the step S3-S6, drying, crushing and sieving to obtain the dendrobium residue powder.

As a further limitation of the above aspect, in step S1, the freeze-drying temperature is-30 to-50 ℃.

As a further limitation of the above, in step S3, the ratio of 1 g: adding 10-50 mL of a material-liquid ratio, adding a disodium hydrogen phosphate-citric acid buffer solution as a simulated gastric juice extractant, adjusting the pH value of a system to 2.0-3.0, fully and uniformly shaking, extracting in a water bath at 40-60 ℃ for 1-2 h, and filtering.

As a further limitation of the scheme, in the step S4, the dosage of the enzyme for destroying the plant cell walls is 0.5-1% of the mass of the filter residue, the shaking extraction temperature is 35-40 ℃, and the time is 1-2 hours.

As a further limitation of the above aspect, the plant cell wall disrupting enzyme is a combination of one or more of cellulase, xylanase, pectinase and beta-glucanase.

In step S5, disodium hydrogen phosphate-citric acid buffer solution is added as simulated intestinal juice extract, the pH of the system is adjusted to 7.5-9.0, the mixture is fully mixed, and the mixture is extracted under reflux at 40-60 ℃ for 1-2 h.

As a further limitation of the above scheme, in step S7, the drying temperature is 40 ℃, and after grinding, the powder is sieved by a 200-mesh sieve.

As a further limitation of the scheme, the abrasive is dendrobium residue powder, pseudo-ginseng powder and green tea powder in a mass ratio of 1: 0.5-1: 1-2.

As a further limitation of the above scheme, the humectant is glycerin, sorbitol in a weight ratio of 1:1 to 3.

As a further limitation of the above scheme, the nano hydroxyapatite is a nano hydroxyapatite with a particle size of 20-50 nm, a nano hydroxyapatite with a particle size of 50-80 nm, a nano hydroxyapatite with a particle size of 80-200nm, and the weight ratio of the nano hydroxyapatite to the nano hydroxyapatite is 1: 1-3: 1 to 3.

As a further limitation of the above scheme, the thickener is carrageenan and sodium alginate in a weight ratio of 1: 2 to 5.

Compared with the prior art, the invention has the beneficial effects that:

(1) compared with the method which only uses the semi-bionic method or the enzyme method, the method has high extraction efficiency, is beneficial to fully exerting the effect of the effective components of the dendrobium, has simple operation and environmental protection, is suitable for industrialized large-scale production, and has better application value.

(2) The invention has the advantages of low cost of raw materials, simple and scientific formula, simple and convenient process, low cost, scientific formula and high cost performance.

(3) The invention uses the crushed dendrobium residue as the abrasive and reasonably combines with other natural components as the abrasive, thereby playing the role of avoiding tooth damage and gingival bleeding, fully utilizing the medicinal value of dendrobium and simultaneously reducing the pollution of the dendrobium residue to the environment.

(4) The low-temperature freeze drying and the crushing machine are adopted for crushing, so that the cell wall breaking rate reaches over 99 percent, the bioactive components of the Chinese herbal medicine are fully reserved, and meanwhile, the method is a technical breakthrough for a single Chinese herbal medicine crushing mode, the utilization rate of the Chinese herbal medicine is improved, and the additional value of the Chinese herbal medicine is increased.

Drawings

FIG. 1 shows the viable cell counts of the toothpastes prepared in examples 1 to 3 and comparative examples 1 to 2 at different times after they act on the biofilm.

FIG. 2 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with chronic atrophic gastritis obtained from the extract of Dendrobium nobile according to examples 1 and 4-11.

FIG. 3 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with chronic atrophic gastritis obtained from the extract of Dendrobium nobile according to examples 1 and 12-15.

FIG. 4 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with the extract of Dendrobium nobile obtained in examples 14-29.

FIG. 5 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with the dendrobium extract prepared in examples 30-35.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly apparent, the present invention is further described in detail with reference to the following embodiments; it should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention; reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

In the following specific examples, the method for measuring the antibacterial and anti-inflammatory effects of the toothpaste is as follows:

culturing oral anaerobic pathogenic bacteria such as Ms, Ss, Lgg, Av, Fn and Pg related to caries, gingivitis, periodontitis and halitosis in an anaerobic workstation to form an oral anaerobic bacteria mixed bacterial biofilm; preparing a suspension with the mass concentration of 0.5g/L by using sterile water for the toothpaste prepared according to the embodiment of the invention, adding 1mL of the suspension into a small hole of a biomembrane pore plate, keeping for 3min, and lightly washing for 3 times (1 mL/time) by using normal saline; and (3) continuously culturing the biological membrane, changing the solution every 1h, namely sucking all the solution of the system, adding 1mL of brain-heart leachate Broth (BHI) enrichment culture medium, and counting the viable bacteria of the biological membrane at the bottom of the small hole by using a flat viable bacteria counting method at 0, 2, 4, 6 and 8h respectively.

Example 1

The antibacterial and anti-inflammatory dendrobium toothpaste comprises the following components in parts by weight: 12 parts of dendrobium extract, 10 parts of wetting agent, 8 parts of abrasive, 0.6 part of potassium sorbate, 0.8 part of thickening agent, 4 parts of lauryl sodium sulfate, 0.3 part of sweetening agent, 10 parts of nano-hydroxyapatite and 20 parts of deionized water.

The extraction method of the dendrobium extract comprises the following steps:

s1, cleaning fresh dendrobium, putting the cleaned fresh dendrobium into a low-temperature drying oven, and carrying out freeze drying at-40 ℃;

s2, pouring the frozen dendrobium into a crusher for crushing;

s3, taking the crushed dendrobium powder, and mixing the crushed dendrobium powder with the raw materials according to the weight ratio of 1 g: adding a disodium hydrogen phosphate-citric acid buffer solution serving as a simulated gastric juice extractant into a feed-liquid ratio of 30mL, adjusting the pH value of a system to 2.5, fully and uniformly shaking, extracting in a water bath at 50 ℃ for 1.5h, and filtering;

s4, adding disodium hydrogen phosphate-citric acid buffer solution into the filter residue obtained in the step S3, adjusting the pH value of the system to 4.0, adding enzyme which is 0.8 percent of the mass of the filter residue and can destroy plant cell walls, and oscillating and extracting for 1.5h at 37 ℃;

wherein the enzyme for destroying the plant cell wall is a complex enzyme consisting of cellulase, xylanase, pectinase and beta-glucanase according to the mass ratio of 1:1:1: 1;

s5, adding a disodium hydrogen phosphate-citric acid buffer solution into the filter residue obtained in the step S4 to serve as a simulated intestinal juice extracting solution, adjusting the pH value of a system to be 8.0, fully and uniformly mixing, performing reflux extraction at 50 ℃ for 1.5h, and centrifuging the extracting solution;

s6, merging the supernatant liquid obtained after centrifugation in the steps S3-S5, and concentrating under reduced pressure to 1/4 of the volume of the stock solution;

s7, mixing the residues obtained in the step S3-S6, drying at 40 ℃, crushing, and sieving with a 200-mesh sieve to obtain the dendrobium residue powder.

Wherein the abrasive is dendrobium residue powder, pseudo-ginseng powder and green tea powder in a mass ratio of 1: 0.8:1.5 mixing; the wetting agent is glycerol and sorbitol according to the weight ratio of 1: 2; the nano-hydroxyapatite is nano-hydroxyapatite with the particle size of 20-50 nm, nano-hydroxyapatite with the particle size of 50-80 nm and nano-hydroxyapatite with the particle size of 80-200nm, wherein the weight ratio of the nano-hydroxyapatite to the nano-hydroxyapatite is 1: 2: 2; the thickening agent is carrageenan and sodium alginate, and the weight ratio is 1: 3 in a mixture of two or more.

Example 2

The embodiment provides an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from the dendrobium toothpaste in embodiment 1 in that the dendrobium toothpaste comprises the following components in parts by weight: 2 parts of dendrobium extract, 15 parts of wetting agent, 5 parts of friction agent, 0.8 part of potassium sorbate, 1 part of thickening agent, 5 parts of sodium dodecyl sulfate, 0.2 part of sweetening agent, 5 parts of nano-hydroxyapatite and 25 parts of deionized water.

Example 3

The embodiment provides an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from the dendrobium toothpaste in embodiment 1 in that the dendrobium toothpaste comprises the following components in parts by weight: 20 parts of dendrobium extract, 5 parts of wetting agent, 10 parts of friction agent, 0.5 part of potassium sorbate, 0.5 part of thickening agent, 2 parts of lauryl sodium sulfate, 0.5 part of sweetening agent, 15 parts of nano hydroxyapatite and 15 parts of deionized water.

Examples 4 to 11

Examples 4 to 11 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the reaction conditions in the step S3 of the method for extracting the dendrobium extract are as shown in the following table, and the rest is the same as example 1.

Examples 12 to 15

Examples 12 to 15 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the extraction method of the dendrobium extract, the freeze-drying conditions in step S1 are as shown in the following table, and the rest is the same as example 1.

Examples	Temperature (. degree. C.)
		Example 12	-20
Example 13	-30
		Example 14	-50
Example 15	-60

Examples 16 to 29

Examples 16 to 29 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the reaction conditions in step S4 of the method for extracting dendrobium extract are as shown in the following table, and the rest is the same as example 1.

Examples 30 to 35

Examples 30 to 35 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the reaction conditions in step S5 of the method for extracting dendrobium extract are as shown in the following table, and the rest is the same as example 1.

Examples	pH value	Temperature (. degree.C.)	Time (h)
				Example 30	7.5	50	1.5
Example 31	9	50	1.5
				Example 32	8	40	1.5
Example 33	8	60	1.5
				Example 34	8	50	1
Example 35	8	50	2

Examples 36 to 38

Examples 36 to 38 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the abrasive is a mixture of dendrobium residue powder, notoginseng powder and green tea powder in the following mass ratio, specifically shown in the following table, and the rest is the same as example 1.

Examples	Mass ratio of
		Example 36	1：0.5：2
Example 37	1：1：1
		Example 38	0：1.8：1.5

The inventor finds out through a large amount of experiments that: when the abrasive is dendrobium residue powder, pseudo-ginseng powder and green tea powder in a mass ratio of 1: when the components are mixed at a ratio of 0.8:1.5, the toothpaste prepared under the condition of the embodiment 1 has the best antibacterial and anti-inflammatory effects, and the antibacterial and anti-inflammatory effects of the toothpaste can be influenced by changing the components and the mass ratio of the abrasive, and in the range limited by the invention, when the abrasive is prepared from dendrobium residue powder, pseudo-ginseng powder and green tea powder according to the mass ratio of 1: 0.5-1: 1-2, the prepared dendrobium toothpaste has good antibacterial and anti-inflammatory effects.

Examples 39 to 42

Examples 39-42 provide an antibacterial and anti-inflammatory dentifrice of dendrobium nobile type, which is comparable to example 1 except that the humectant is a mixture of glycerin and sorbitol in the weight ratios shown in the following table, which are otherwise the same as example 1.

The inventor finds out through a large number of experiments that: when the wetting agent is glycerol and sorbitol, the weight ratio of the glycerol to the sorbitol is 1: 2, the toothpaste prepared under the condition of the example 1 has the best antibacterial and anti-inflammatory effects, and the antibacterial and anti-inflammatory effects of the toothpaste can be affected by changing the weight ratio of the glycerol to the sorbitol, and within the range defined by the invention, when the weight ratio of the glycerol to the sorbitol is 1: 1-3, the prepared dendrobium toothpaste has good antibacterial and anti-inflammatory effects.

Examples 43 to 47

Embodiments 43 to 47 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from embodiment 1 in that the nano-hydroxyapatite is a mixture of nano-hydroxyapatite with a particle size of 20 to 50nm, nano-hydroxyapatite with a particle size of 50 to 80nm, and nano-hydroxyapatite with a particle size of 80 to 200nm according to the following weight ratio, as shown in the following table, the rest is the same as embodiment 1.

Examples	Weight ratio of
		Example 43	1:1:3
Example 44	1:3:1
		Example 45	1:0:4
Example 46	1:4:0
		Example 47	5:0:0

The inventor finds out through a large number of experiments that: when the nano hydroxyapatite is nano hydroxyapatite with the particle size of 20-50 nm, nano hydroxyapatite with the particle size of 50-80 nm and nano hydroxyapatite with the particle size of 80-200nm according to the weight ratio of 1: 2: 2, the toothpaste prepared under the conditions of the embodiment 1 has the best antibacterial and anti-inflammatory effects, and the antibacterial and anti-inflammatory effects of the toothpaste can be affected by changing the weight ratio of the nano-hydroxyapatite with different particle sizes, wherein the nano-hydroxyapatite is nano-hydroxyapatite with the particle size of 20-50 nm, nano-hydroxyapatite with the particle size of 50-80 nm and nano-hydroxyapatite with the particle size of 80-200nm according to the weight ratio of 1: 1-3: 1-3, the prepared dendrobium toothpaste can generate better antibacterial and anti-inflammatory effects.

Examples 48 to 51

Examples 48 to 51 provide an antibacterial and anti-inflammatory dendrobium toothpaste, which is different from example 1 in that the thickener is a mixture of carrageenan and sodium alginate in the following weight ratio, as shown in the following table, and the rest is the same as example 1.

Examples	Weight ratio of
		Example 48	1:2
Example 49	1:5
		Example 50	1:1
Example 51	1:6

The inventor finds out through a large number of experiments that: when the thickening agent is carrageenan and sodium alginate, the weight ratio of the carrageenan to the sodium alginate is 1: 3, the toothpaste prepared under the condition of the embodiment 1 has the best antibacterial and anti-inflammatory effects, the antibacterial and anti-inflammatory effects of the toothpaste can be affected by changing the weight ratio of the carrageenan to the alginic acid, and the carrageenan and the sodium alginate are mixed according to the weight ratio of 1: the mixture of 2-5 can produce better antibacterial and anti-inflammatory effects.

Comparative example 1

The comparison example provides an antibacterial and anti-inflammatory dendrobium nobile toothpaste, which is different from the dendrobium nobile toothpaste in the example 1 in that the extraction method of the dendrobium nobile extract is the same as the example 1 except that no enzyme for destroying plant cell walls is added in the step S3.

Comparative example 2

The comparison example provides an antibacterial and anti-inflammatory dendrobium nobile toothpaste, and compared with example 1, the difference is that the extraction method of the dendrobium nobile extract comprises the following steps:

s2, pouring the frozen dendrobium into a crusher for crushing;

s3, taking the crushed dendrobium powder, and mixing the crushed dendrobium powder with the raw materials according to the weight ratio of 1 g: adding deionized water into the mixture at a feed-liquid ratio of 30mL, fully and uniformly shaking, extracting in a water bath at 50 ℃ for 1.5h, and filtering;

s5, adding deionized water into the filter residue obtained in the step S4, fully and uniformly mixing, carrying out reflux extraction at 50 ℃ for 1.5h, and centrifuging the extracting solution;

Test example 1

Culturing oral anaerobic pathogenic bacteria such as Ms, Ss, Lgg, Av, Fn and Pg related to caries, gingivitis, periodontitis and halitosis in an anaerobic workstation to form an oral anaerobic bacteria mixed bacterial biofilm; preparing the toothpaste prepared according to the formula amounts of the examples 1-3 and the comparative examples 1-2 into a suspension with the mass concentration of 0.5g/L by using sterile water, adding 1mL of the suspension into a small hole of a biomembrane pore plate, keeping for 3min, and lightly washing for 3 times (1 mL/time) by using normal saline; and (3) continuously culturing the biological membrane, changing the solution every 1h, namely sucking all the solution of the system, adding 1mL of brain-heart leachate Broth (BHI) enrichment culture medium, and counting the viable bacteria of the biological membrane at the bottom of the small hole by using a flat viable bacteria counting method at 0, 2, 4, 6 and 8h respectively.

FIG. 1 is a result graph of viable count of the toothpaste prepared in examples 1-3 after acting on a biofilm at different times, and it can be seen from the results in the graph that the viable count increases as the bacteria continuously proliferate with the time; after the toothpaste prepared in the example 1 is applied to a biological membrane for 3min, the number of the living bacteria is small at 0 and 2h, and the number of the living bacteria is continuously increased along with the prolonging of the time; after the toothpaste in the embodiment 2 and the embodiment 3 is applied to the biological membrane for 3min, the number of the living bacteria is higher than that of the living bacteria applied in the embodiment 1 in 0 and 2h, but the number of the living bacteria applied to the biological membrane in the toothpaste in the embodiments 1 to 3 is slowly increased along with the prolonging of time, which shows that the toothpaste prepared by the invention has a long-acting antibacterial effect; compared with the semi-bionic method or the enzyme method which is used alone, the toothpaste prepared in the comparative examples 1 and 2 has more viable count and the viable count increases rapidly along with the time extension, which shows that the extraction efficiency is higher by extracting the active ingredients in the dendrobium through the combination of the semi-bionic method and the biological enzyme, and the efficacy of the effective ingredients of the dendrobium is brought into full play.

Test example 2

The rats are randomly divided into a normal group, a model group, a dendrobe dosage group and a Yangweishu group according to the weight, and each group comprises 20 rats; the model group, the dendrobium dosage group and the stomach nourishing group are given 167 mu g/mL MNNG drinking liquid (filled into a 500mL black water bottle and freely drunk), the normal group freely drinks distilled water, the model is built for 8 weeks, and after the histopathological examination determines that the model building is successful, the dendrobium dosage group is fed with the dendrobium extract prepared by the method every day by intragastric administration, and the dosage is 1.34 g/kg; 4.0g/kg of Yangweishu granules are taken in the Yangweishu group by intragastric administration every day; the model group was subjected to gavage with physiological saline, the normal group was not intervened, and after 12 weeks of administration, 4 groups of rats were subjected to abdominal anesthesia, stomach tissues were taken out, fixed and stored by immersion in formalin.

And detecting the content of gastrin GAS and interleukin IL-6 in the serum of the mouse by referring to an enzyme-linked immunosorbent assay (ELISA) detection kit.

Fig. 2 shows the content of gastrin GAS and interleukin IL-6 in rats treated with chronic atrophic gastritis by the dendrobium extract prepared in examples 1 and 4 to 11 for 12 weeks, and the results in the figure show that the GAS content in the normal group, the dendrobium extract dose group prepared in the example of the present invention, and the gastricism group are higher than those in the model group; the IL-6 content of the normal group and the dendrobium extract dosage group prepared by the embodiment of the invention is lower than that of the model group; compared with the rats of experimental groups of examples 4 to 11, after the treatment of the dendrobium extract dose group prepared in example 1, the content of gastrin GAS and interleukin IL-6 in the serum of the rat is close to that in the serum of a normal rat, which indicates that the dendrobium extract prepared in example 1 has the best treatment effect on the chronic atrophic gastritis of the rat, and the change of any condition in the step S3 in the dendrobium extract extraction method can affect the extraction efficiency of the dendrobium extract, thereby affecting the treatment effect of the dendrobium extract on the chronic atrophic gastritis of the rat.

Fig. 3 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated with chronic atrophic gastritis by the dendrobium extract prepared in example 1 and examples 12 to 15 for 12 weeks, and the results in the figure show that the GAS content in the normal group, the dendrobium extract dose group and the yangwshu group prepared in the example of the present invention are higher than those in the model group; the IL-6 content of the normal group and the dendrobium extract dosage group prepared by the embodiment of the invention is lower than that of the model group; compared with the rats of experimental groups in examples 12 to 15, after the treatment of the dendrobium extract dose group prepared in example 1, the content of gastrin GAS and interleukin IL-6 in the serum of the rat is close to that in the serum of a normal rat, which indicates that the dendrobium extract prepared in example 1 has the best treatment effect on the chronic atrophic gastritis of the rat, and the dendrobium extract prepared in examples 13 to 14 has better treatment effect on the chronic atrophic gastritis of the rat than those of examples 12 and 15, and indicates that changing the freeze-drying temperature of step S1 in the dendrobium extraction method can affect the extraction efficiency of the dendrobium extract, thereby affecting the treatment effect of the dendrobium extract on the chronic atrophic gastritis of the rat.

FIG. 4 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with chronic atrophic gastritis by the dendrobium extract prepared in examples 14-29, and the results in the figure show that the dose groups of the dendrobium extract prepared in the examples of the present invention are all higher than the dose groups of the model groups; the content of IL-6 in the dendrobium extract dose group prepared by the embodiment of the invention is lower than that of the model group; the treatment effect of the dendrobium extract prepared in the example 1 on the rat chronic atrophic gastritis is better than that of the examples 14 to 29, which shows that the change of the reaction conditions in the step S4 in the dendrobium extraction method affects the extraction efficiency of the dendrobium extract, so that the treatment effect of the dendrobium extract on the rat chronic atrophic gastritis is affected.

FIG. 5 shows the content of serum gastrin GAS and interleukin IL-6 in rats treated for 12 weeks with chronic atrophic gastritis by the dendrobium extract prepared in examples 30-35, and the results in the figure show that the dose groups of the dendrobium extract prepared in the examples of the present invention are all higher than the dose groups of the model groups; the content of IL-6 in the dendrobium extract dose group prepared by the embodiment of the invention is lower than that of the model group; the treatment effect of the dendrobium extract prepared in the embodiment 1 on the rat chronic atrophic gastritis is better than that of the embodiment 30-35, which shows that the change of the reaction conditions in the extraction process of the simulated intestinal juice in the step S5 in the dendrobium extraction method can affect the extraction efficiency of the dendrobium extract, so that the treatment effect of the dendrobium extract on the rat chronic atrophic gastritis is affected.

While the invention has been described with respect to specific embodiments thereof, it will be understood by those skilled in the art that the foregoing and other changes, omissions and deviations in the form and detail thereof may be made without departing from the scope of this invention; those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention; meanwhile, any equivalent changes, modifications and alterations of the above embodiments according to the spirit and techniques of the present invention are also within the scope of the present invention.

Claims

1. The antibacterial and anti-inflammatory dendrobium toothpaste is characterized by comprising the following components in parts by weight: 2-20 parts of dendrobium extract, 5-15 parts of wetting agent, 5-10 parts of friction agent, 0.5-0.8 part of potassium sorbate, 0.5-1.0 part of thickening agent, 2-5 parts of sodium dodecyl sulfate, 0.2-0.5 part of sweetening agent, 5-15 parts of nano hydroxyapatite and 15-25 parts of deionized water;

the extraction method of the dendrobium extract comprises the following steps:

s1, cleaning fresh dendrobium, and then putting the dendrobium into a low-temperature drying oven for freeze drying; the freeze drying temperature is-30 to-50 ℃;

s2, pouring the frozen dendrobium into a crusher for crushing;

s3, taking the crushed dendrobium powder, extracting the dendrobium powder by simulated gastric juice, and mixing the crushed dendrobium powder with the simulated gastric juice according to the weight ratio of 1 g: adding a disodium hydrogen phosphate-citric acid buffer solution serving as a simulated gastric juice extractant at a material-liquid ratio of 10-50 mL, adjusting the pH value of a system to 2.0-3.0, sufficiently and uniformly shaking, extracting in a water bath at 40-60 ℃ for 1-2 h, filtering, and centrifuging an extracting solution;

s4, adding disodium hydrogen phosphate-citric acid buffer solution into the filter residue obtained in the step S3, adjusting the pH value of the system to 3.0-5.0, adding enzyme for destroying plant cell walls, and performing oscillation extraction; the enzyme for destroying the plant cell wall is a complex enzyme consisting of cellulase, xylanase, pectinase and beta-glucanase according to the mass ratio of 1:1:1: 1;

s5, dissolving the filter residue obtained in the step S4 in simulated intestinal juice for extraction, adding a disodium hydrogen phosphate-citric acid buffer solution as a simulated intestinal juice extracting solution, adjusting the pH value of a system to be 7.5-9.0, fully and uniformly mixing, performing reflux extraction at 40-60 ℃ for 1-2 hours, and centrifuging the extracting solution;

s6, merging the supernatants obtained after centrifugation in the steps S3-S5, and concentrating under reduced pressure to 1/3-1/5 of the volume of the stock solution;

s7, combining the medicine residues obtained in the step S3-S6, drying, crushing and sieving to obtain dendrobium residue powder;

the abrasive is prepared from dendrobium residue powder, pseudo-ginseng powder and green tea powder in a mass ratio of 1: 0.5-1: 1-2.

2. The antibacterial and anti-inflammatory dendrobium nobile toothpaste according to claim 1, wherein in the step S4, the amount of the enzyme for destroying the plant cell walls is 0.5-1% of the mass of the filter residue, the shaking extraction temperature is 35-40 ℃, and the shaking extraction time is 1-2 hours.

3. The antibacterial and anti-inflammatory dendrobium nobile toothpaste as claimed in claim 1, wherein in step S7, the drying temperature is 40 ℃, and after crushing, the dendrobium nobile toothpaste is sieved by a 200-mesh sieve.

4. The antibacterial and anti-inflammatory dendrobium toothpaste according to claim 1, wherein the nano-hydroxyapatite is nano-hydroxyapatite with the particle size of 20-50 nm, nano-hydroxyapatite with the particle size of 50-80 nm, and nano-hydroxyapatite with the particle size of 80-200nm according to the weight ratio of 1: 1-3: 1 to 3.