CN110051596A - A kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory - Google Patents
A kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory Download PDFInfo
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Abstract
The invention discloses a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, in parts by weight include following components: 2~20 parts of Herba Dendrobii extract, 5~15 parts of wetting agent, 5~10 parts of rubbing agent, 0.5~0.8 part of potassium sorbate, 0.5~1.0 part of thickener, 2~5 parts of lauryl sodium sulfate, 0.2~0.5 part of sweetener, 5~15 parts of nanometer hydroxyapatite, 15~25 parts of deionized water；Cost of material of the present invention is low, the simple science of formula, half biomimetic method and biological enzyme are combined to the active constituent extracted in dendrobium nobile simultaneously, gastrointestinal tract is interior in vivo after simulation dendrobium nobile is oral absorbs transport process, and this method is compared with being used alone half biomimetic method or enzyme process, extraction efficiency is high, be conducive to the effect of giving full play to dendrobium nobile effective component, and easy to operate, it is environmental-friendly, it is suitble to industrialization large-scale production, there is preferable application value.
Description
Technical field
The present invention relates to daily necessities fields, and in particular to a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory.
Background technique
The main pathological change of atrophic gastritis be the atrophy of mucous membrane body of gland, thinning, inflammation, can be with enteraden epithelialization
Raw and atypical hyperplasia, disease incidence account for the 10~20% of whole chronic gastritis, and the World Health Organization has been classified as shape before gastric cancer
State.
Toothpaste is indispensable oral cleaning amenities in people's daily life, with the increasing of people's health care consciousness
By force, requirement of the consumer to toothpaste is higher and higher, and the functional form toothpaste for adding active ingredient of Chinese herbs becomes a master in market
Developing direction is flowed, but more existing is not obvious by the effect of toothpaste of active constituent of Chinese medicine.
Dendrobium nobile is a kind of rare traditional Chinese medicine, and Shennong's Herbal is said " in main wound；Eliminating impediment, lower gas；Tonifying five zang organs consumptive disease is won, by force
Yin, long term usage thickness stomach ", can nourishing yin for benefiting stomach, clearing heat and promoting fluid；Dendrobium nobile is rich in polysaccharide, flavones, alkaloid, free amino acid, vitamin
And the ingredients such as various enzymes；Research finds that dendrobium polysaccharide has the effects that anti-oxidant, antitumor, anti-inflammatory, enhancing is immune；To master
Medicine finds that the medicine can promote rat to secrete gastric juice in the research for the maple bucket crystalline substance of dendrobium nobile, increase the discharge of gastric acid, pepsin
Amount；And external application can stop blooding, promote wound healing；The active constituent of dendrobium nobile is effectively combined with toothpaste, oral cavity may be implemented
Disease prevention and nursing health, while preventing atrophic gastritis, reduce the incidence of gastric cancer.
Existing Herba Dendrobii extract preparation method be mostly water extract or alcohol extracting, organic solvent extract, ultrasonic extraction and super
The methods of critical extraction is made；It adopts merely and is extracted with water or the method for alcohol extracting, extraction time is long, expends reagent, and cannot be complete
Will have bioactive ingredients the effect of extracting completely, dendrobium nobile cannot be fully demonstrated effect in dendrobium nobile；Using ultrasound side
Method is extracted, and there are ultrasonic attenuation, noise is big, radiates the disadvantages of strong, is not suitable for industrialized production；Using the side of supercritical extract
Herba Dendrobii extract made from method comes out the chemical component extraction that molecular weight is big, polarity is big not, and recovery rate is low, and production cost
It is excessively high.
The dissolution of active ingredients from traditional Chinese medicinal also has close relationship with its degree of grinding, as the raising of sporoderm-broken rate can be with
The dissolution for improving effective component, improves the utility value of Chinese medicine, improves the quality of product；Dendrobium nobile is added in toothpaste, is expected to
Improve disease prevention and nursing health；However, the effect of how playing dendrobium nobile to greatest extent and deep processing are applied to as people
It is dedicated to the problem of research in recent years；Currently, relatively conventional dendrobium nobile addition manner is to be made into Dendrobium, add in medicine
In agent, still, the mode cell-wall breaking ratio that Dendrobium is made in common mode is low, causes the overall utilization rate of medicinal material lower,
Rate of release in human body and Chinese materia medica preparation is low, further influences the performance of drug effect.
Summary of the invention
In response to the problems existing in the prior art, the purpose of the present invention is to provide a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, resist
Bacterium anti-inflammatory effects are good, all have good curative effect for various focusing depths represented, bleeding, canker sore, and are used for a long time and can be improved pair
The disease prevention of atrophic gastritis.
To achieve the above object, the technical solution adopted by the present invention is that:
A kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory includes following components in parts by weight: 2~20 parts of Herba Dendrobii extract, wetting
5~15 parts of agent, 5~10 parts of rubbing agent, 0.5~0.8 part of potassium sorbate, 0.5~1.0 part of thickener, lauryl sodium sulfate 2~
5 parts, 0.2~0.5 part of sweetener, 5~15 parts of nanometer hydroxyapatite, 15~25 parts of deionized water.
As further limiting for above scheme, the extracting method of the Herba Dendrobii extract includes the following steps:
S1, by fresh dendrobium nobile, be put into low temperature drying case after cleaning and be freeze-dried；
S2, it the dendrobium nobile of freezing is poured into crusher is crushed；
S3, smashed dendrobe powder is taken, is extracted with simulate the gastric juice, extracting solution centrifugation；
S4, disodium hydrogen phosphate-citric acid solution is added in the resulting filter residue of step S3, regulation system pH value is
3.0~5.0, the enzyme for destroying plant cell wall, mechanical shaking extraction is added；
S5, simulated intestinal fluid is dissolved in the resulting filter residue of step S4 extracts, extracting solution centrifugation；
S6, the supernatant liquid obtained after step S3~S5 centrifugation is merged, is concentrated under reduced pressure into the 1/3~1/5 of stoste volume；
S7, the dregs of a decoction for obtaining step S3~S6 merge, and drying crushes, dendrobium nobile dregs of a decoction powder is made after sieving.
As further limiting for above scheme, in step S1, freeze-drying temperature is -30~-50 DEG C.
As further limiting for above scheme, in step S3, according to the solid-liquid ratio of 1g:10~50mL, phosphoric acid hydrogen is added
For disodium-citric acid solution as simulate the gastric juice extractant, regulation system pH value is 2.0~3.0, fully shake uniformly, in
40~60 DEG C of water-baths are filtered after extracting 1~2h.
As further limiting for above scheme, in step S4, the enzyme dosage for destroying plant cell wall is filter residue quality
0.5~1%, concussion Extracting temperature is 35~40 DEG C, and the time is 1~2h.
As further limiting for above scheme, the enzyme for destroying plant cell wall is cellulase, zytase, fruit
One of glue enzyme and 1,4 beta-glucanase or a variety of combinations.
As further limiting for above scheme, in step S5, disodium hydrogen phosphate-citric acid solution is added as mould
Quasi- intestinal juice extracting solution, regulation system pH value are 7.5~9.0, are mixed well, 1~2h of refluxing extraction at 40~60 DEG C.
As further limiting for above scheme, in step S7, drying temperature is 40 DEG C, smashes it through 200 meshes.
As further limiting for above scheme, the rubbing agent be dendrobium nobile dregs of a decoction powder, Radix Notoginseng powder, green tea powder in mass ratio
It is mixed for 1:0.5~1:1~2.
As further limiting for above scheme, the wetting agent is glycerol, sorbierite is the mixed of 1:1~3 by weight
Close object.
As further limiting for above scheme, the nanometer hydroxyapatite is the nano-hydroxy that partial size is 20~50nm
The nanometer hydroxyapatite that nanometer hydroxyapatite that apatite, partial size are 50~80nm, partial size are 80-200nm is by weight
For the mixture of 1:1~3:1~3.
As further limiting for above scheme, the thickener is carragheen, sodium alginate is 1:2~5 by weight
Mixture.
Compared with prior art, the beneficial effects of the present invention are:
(1) half biomimetic method and biological enzyme are combined the active constituent extracted in dendrobium nobile by the present invention simultaneously, simulate dendrobium nobile
Gastrointestinal tract is interior in vivo after oral absorbs transport process, and this method is compared with being used alone half biomimetic method or enzyme process, extraction efficiency
Height is conducive to the effect of giving full play to dendrobium nobile effective component, and easy to operate, environmental-friendly, is suitble to industrialization large-scale production,
With preferable application value.
(2) cost of material of the present invention is low, is formulated simple science, and simple process is low in cost, formula science, and cost performance is high.
(3) present invention dendrobium nobile is extracted after the dregs of a decoction crush after be used as rubbing agent, and with other natural component reasonable compatibilities
As rubbing agent, the effect of avoiding injury of teeth and bleeding gums can be played, so that the medical value of dendrobium nobile has obtained abundant benefit
With, while reducing pollution of the dendrobium nobile dregs of a decoction to environment.
(4) it is crushed using frozen drying and crusher, makes cell-wall breaking ratio up to 99% or more, be sufficiently reserved
Its bioactive ingredients improves medicinal material simultaneously for being a kind of technical breakthrough for single Chinese herbal medicine grinding mode
Utilization rate increases its added value.
Detailed description of the invention
Fig. 1 is the work that toothpaste made from EXAMPLE Example 1~3 and comparative example 1~2 acts on different time after biomembrane
Bacterium number.
Fig. 2 is embodiment 1, Herba Dendrobii extract made from embodiment 4~11 treats atrophic gastritis rat 12 weeks
Rat blood serum gastrin GAS, Interleukin-6 content.
Fig. 3 is embodiment 1, Herba Dendrobii extract made from embodiment 12~15 treats atrophic gastritis rat 12 weeks
Rat blood serum gastrin GAS, Interleukin-6 content.
Fig. 4 is that Herba Dendrobii extract made from embodiment 14~29 treats 12 weeks rat blood serums to atrophic gastritis rat
Gastrin GAS, Interleukin-6 content.
Fig. 5 is that Herba Dendrobii extract made from embodiment 30~35 treats 12 weeks rat blood serums to atrophic gastritis rat
Gastrin GAS, Interleukin-6 content.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated；It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention；Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method
And equipment.
In following specific embodiments, the antibacterial and anti-inflammatory effects measuring method of toothpaste is as follows:
Ms, Ss, Lgg, Av, Fn and Pg etc. and dental caries, gingivitis, periodontitis and halitosis disease phase are cultivated in anaerobism work station
The oral cavity anaerobism pathogenic bacteria of pass form oral cavity anaerobium Mixed Microbes biomembrane；It will be used according to toothpaste made from the embodiment of the present invention
It is 0.5g/L suspension that sterile water, which is configured to mass concentration, and 1mL is taken to be added in the aperture of biomembrane orifice plate, retains 3min, with life
Reason salt water is gently washed 3 times (1mL/ times)；Continuing the culture of biomembrane, every 1h is changed the liquid once, that is, all solution of system are sucked,
1mL brain heart oxoid meat soup (BHI) enriched medium is added, respectively at 0,2,4,6 and 8h with viable plate count method to small
The biomembrane of hole bottom carries out count plate.
Embodiment 1
A kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory includes following components: 12 parts of Herba Dendrobii extract, wetting agent 10 in parts by weight
Part, 8 parts of rubbing agent, 0.6 part of potassium sorbate, 0.8 part of thickener, 4 parts of lauryl sodium sulfate, 0.3 part of sweetener, nano-hydroxy
10 parts of apatite, 20 parts of deionized water.
The extracting method of the Herba Dendrobii extract, includes the following steps:
S1, by fresh dendrobium nobile, be put into low temperature drying case after cleaning, be freeze-dried in -40 DEG C；
S2, it the dendrobium nobile of freezing is poured into crusher is crushed；
S3, smashed dendrobe powder is taken, according to the solid-liquid ratio of 1g:30mL, disodium hydrogen phosphate-citric acid solution is added
As simulate the gastric juice extractant, regulation system pH value is 2.5, fullys shake uniformly, filters after 1.5h is extracted in 50 DEG C of water-baths；
S4, disodium hydrogen phosphate-citric acid solution is added in the resulting filter residue of step S3, regulation system pH value is
4.0, the enzyme of the destruction plant cell wall of filter residue quality 0.8% is added, in 37 DEG C of mechanical shaking extraction 1.5h；
Wherein, the enzyme for destroying plant cell wall is that cellulase, zytase, pectase and 1,4 beta-glucanase press matter
Amount is than the complex enzyme for 1:1:1:1 composition；
S5, disodium hydrogen phosphate-citric acid solution is added as simulated intestinal fluid extracting solution in the resulting filter residue of step S4,
Regulation system pH value is 8.0, is mixed well, the refluxing extraction 1.5h at 50 DEG C, extracting solution centrifugation；
S6, the supernatant liquid obtained after step S3~S5 centrifugation is merged, is concentrated under reduced pressure into the 1/4 of stoste volume；
S7, the dregs of a decoction for obtaining step S3~S6 merge, and the dendrobium nobile dregs of a decoction are made after smashing it through 200 meshes in 40 DEG C of drying
Powder.
Wherein, the rubbing agent is dendrobium nobile dregs of a decoction powder, Radix Notoginseng powder, green tea powder are that 1:0.8:1.5 is mixed in mass ratio；
The wetting agent is the mixture that glycerol, sorbierite are 1:2 by weight；The nanometer hydroxyapatite be partial size be 20~
The nano-hydroxy that nanometer hydroxyapatite that nanometer hydroxyapatite, the partial size of 50nm is 50~80nm, partial size are 80-200nm
Apatite is the mixture of 1:2:2 by weight；The thickener is the mixing that carragheen, sodium alginate are 1:3 by weight
Object.
Embodiment 2
The present embodiment provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, by weight
Number includes following components: 2 parts of Herba Dendrobii extract, 15 parts of wetting agent, 5 parts of rubbing agent, 0.8 part of potassium sorbate, 1 part of thickener,
5 parts of lauryl sodium sulfate, 0.2 part of sweetener, 5 parts of nanometer hydroxyapatite, 25 parts of deionized water.
Embodiment 3
The present embodiment provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, by weight
Number includes following components: 20 parts of Herba Dendrobii extract, 5 parts of wetting agent, 10 parts of rubbing agent, 0.5 part of potassium sorbate, thickener 0.5
Part, 2 parts of lauryl sodium sulfate, 0.5 part of sweetener, 15 parts of nanometer hydroxyapatite, 15 parts of deionized water.
Embodiment 4-11
Embodiment 4-11 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
The extracting method of Herba Dendrobii extract, in step S3 shown in reaction condition table specific as follows, remaining is all the same with embodiment 1.
Embodiment 12-15
Embodiment 12-15 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
The extracting method of Herba Dendrobii extract, in step S1, freeze-drying condition is as shown in the table, remaining is all the same with embodiment 1.
| Embodiment | Temperature (DEG C) |
| Embodiment 12 | -20 |
| Embodiment 13 | -30 |
| Embodiment 14 | -50 |
| Embodiment 15 | -60 |
Embodiment 16-29
Embodiment 16-29 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
The extracting method of Herba Dendrobii extract, in step S4, shown in reaction condition table specific as follows, remaining is all the same with embodiment 1.
Embodiment 30-35
Embodiment 30-35 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
The extracting method of Herba Dendrobii extract, in step S5, shown in reaction condition table specific as follows, remaining is all the same with embodiment 1.
| Embodiment | PH value | Temperature (DEG C) | Time (h) |
| Embodiment 30 | 7.5 | 50 | 1.5 |
| Embodiment 31 | 9 | 50 | 1.5 |
| Embodiment 32 | 8 | 40 | 1.5 |
| Embodiment 33 | 8 | 60 | 1.5 |
| Embodiment 34 | 8 | 50 | 1 |
| Embodiment 35 | 8 | 50 | 2 |
Embodiment 36-38
Embodiment 36-38 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
Rubbing agent is dendrobium nobile dregs of a decoction powder, the mass ratio in Radix Notoginseng powder, green tea powder according to the form below mixes, shown in table specific as follows, remaining
It is all the same with embodiment 1.
| Embodiment | Mass ratio |
| Embodiment 36 | 1:0.5:2 |
| Embodiment 37 | 1:1:1 |
| Embodiment 38 | 0:1.8:1.5 |
Inventor by many experiments find: when the rubbing agent be dendrobium nobile dregs of a decoction powder, Radix Notoginseng powder, green tea powder in mass ratio
When for 1:0.8:1.5 mixing, the antibacterial and anti-inflammatory effects of toothpaste obtained are best under the conditions of embodiment 1, and change rubbing agent at
Divide and quality proportioning can have an impact the antibacterial and anti-inflammatory effects of toothpaste, and in the framework of the present definition, works as rubbing agent
By dendrobium nobile dregs of a decoction powder, Radix Notoginseng powder, green tea powder be in mass ratio 1:0.5~1:1~2 mix when, dendrobium nobile toothpaste obtained all has
Preferable antibacterial and anti-inflammatory effects.
Embodiment 39-42
Embodiment 39-42 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
Wetting agent is the mixture that glycerol, sorbierite press the weight ratio in table, and shown in table specific as follows, remaining is equal with embodiment 1
It is identical.
Inventor has found by many experiments: when the wetting agent is the mixture that glycerol, sorbierite are 1:2 by weight
When, the antibacterial and anti-inflammatory effects of toothpaste obtained are best under the conditions of embodiment 1, and change glycerol, the weight proportion of sorbierite can
The antibacterial and anti-inflammatory effects of toothpaste are had an impact, and in the framework of the present definition, when glycerol, sorbierite are 1 by weight:
When 1~3 mixture, dendrobium nobile toothpaste obtained all has preferable antibacterial and anti-inflammatory effects.
Embodiment 43-47
Embodiment 43-47 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
Nanometer hydroxyapatite be partial size be 20~50nm nanometer hydroxyapatite, partial size be 50~80nm nano-hydroxy-apatite
Stone, the nanometer hydroxyapatite that partial size is 80-200nm press the mixture of following weight ratio, shown in table specific as follows, remaining and reality
It is all the same to apply example 1.
| Embodiment | Weight ratio |
| Embodiment 43 | 1:1:3 |
| Embodiment 44 | 1:3:1 |
| Embodiment 45 | 1:0:4 |
| Embodiment 46 | 1:4:0 |
| Embodiment 47 | 5:0:0 |
Inventor has found by many experiments: when the nanometer hydroxyapatite is the nano-hydroxy that partial size is 20~50nm
The nanometer hydroxyapatite that nanometer hydroxyapatite that apatite, partial size are 50~80nm, partial size are 80-200nm is by weight
When for 1:2:2, the antibacterial and anti-inflammatory effects of toothpaste obtained are best under the conditions of embodiment 1, and change nanometer hydroxyapatite difference
The weight proportion of partial size can have an impact the antibacterial and anti-inflammatory effects of toothpaste, and nanometer hydroxyapatite be partial size be 20~
The nano-hydroxy that nanometer hydroxyapatite that nanometer hydroxyapatite, the partial size of 50nm is 50~80nm, partial size are 80-200nm
When apatite is the mixture of 1:1~3:1~3 by weight, dendrobium nobile toothpaste obtained can generate preferable antibacterial anti-inflammatory effect
Fruit.
Embodiment 48-51
Embodiment 48-51 provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, it is described
Thickener is carragheen, sodium alginate by the mixture of following weight ratio, and shown in table specific as follows, remaining is homogeneous with embodiment 1
Together.
| Embodiment | Weight ratio |
| Embodiment 48 | 1:2 |
| Embodiment 49 | 1:5 |
| Embodiment 50 | 1:1 |
| Embodiment 51 | 1:6 |
Inventor has found by many experiments: when thickener is carragheen, sodium alginate by the mixing for being by weight 1:3
When object, the antibacterial and anti-inflammatory effects of toothpaste obtained are best under the conditions of embodiment 1, and change the weight proportion of carragheen, alginic acid
The antibacterial and anti-inflammatory effects of toothpaste can be had an impact, and when carragheen, sodium alginate are the mixture of 1:2~5 by weight
Preferable antibacterial and anti-inflammatory effects can be generated.
Comparative example 1
This comparative example provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, the stone
The extracting method of dry measure used in former times extract is not added with the enzyme for destroying plant cell wall in step S3, remaining is all the same with embodiment 1.
Comparative example 2
This comparative example provides a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, compared with Example 1, the difference is that, the stone
The extracting method of dry measure used in former times extract, includes the following steps:
S1, by fresh dendrobium nobile, be put into low temperature drying case after cleaning, be freeze-dried in -40 DEG C；
S2, it the dendrobium nobile of freezing is poured into crusher is crushed；
S3, take smashed dendrobe powder, according to the solid-liquid ratio of 1g:30mL, deionized water be added, fully shake uniformly, in
50 DEG C of water-baths are filtered after extracting 1.5h；
S4, disodium hydrogen phosphate-citric acid solution is added in the resulting filter residue of step S3, regulation system pH value is
4.0, the enzyme of the destruction plant cell wall of filter residue quality 0.8% is added, in 37 DEG C of mechanical shaking extraction 1.5h；
Wherein, the enzyme for destroying plant cell wall is that cellulase, zytase, pectase and 1,4 beta-glucanase press matter
Amount is than the complex enzyme for 1:1:1:1 composition；
S5, deionized water is added in the resulting filter residue of step S4, mixes well, the refluxing extraction 1.5h at 50 DEG C, extracts
Liquid centrifugation；
S6, the supernatant liquid obtained after step S3~S5 centrifugation is merged, is concentrated under reduced pressure into the 1/4 of stoste volume；
S7, the dregs of a decoction for obtaining step S3~S6 merge, and the dendrobium nobile dregs of a decoction are made after smashing it through 200 meshes in 40 DEG C of drying
Powder.
Test example 1
Ms, Ss, Lgg, Av, Fn and Pg etc. and dental caries, gingivitis, periodontitis and halitosis disease phase are cultivated in anaerobism work station
The oral cavity anaerobism pathogenic bacteria of pass form oral cavity anaerobium Mixed Microbes biomembrane；It will be by Examples 1 to 3,1~2 formula ratio of comparative example
It is 0.5g/L suspension that toothpaste obtained, which is configured to mass concentration with sterile water, and 1mL is taken to be added in the aperture of biomembrane orifice plate, is protected
3min is stayed, is gently washed with physiological saline 3 times (1mL/ times)；Continue the culture of biomembrane, every 1h is changed the liquid once, that is, sucks body
It is all solution, adds 1mL brain heart oxoid meat soup (BHI) enriched medium, respectively at 0,2,4,6 and 8h with plate viable bacteria
Counting method carries out count plate to the biomembrane of aperture bottom.
Fig. 1 is the viable count result figure that toothpaste made from Examples 1 to 3 acts on different time after biomembrane, You Tuzhong
As a result it is found that with the extension of time, bacterium constantly breeds, viable count is caused to be continuously increased；Use toothpaste made from embodiment 1
After acting on biomembrane 3min, at 0 and 2h, viable count is fewer, with time extension, viable count is continuously increased；It uses
After embodiment 2,3 toothpaste of embodiment effect biomembrane 3min, at 0 and 2h, viable count is higher than the viable bacteria after embodiment 1 acts on
Number, but with the extension of time, Examples 1 to 3 toothpaste effect biomembrane after viable count increase slowly, illustrate system of the present invention
The toothpaste obtained has long-acting bacteriostatic effect；And toothpaste viable count made from comparative example 1, comparative example 2 is more and extends at any time,
Viable count increases sharply, and illustrates that compared with half biomimetic method or enzyme process is used alone, the present invention passes through half biomimetic method and biological enzyme knot
The active constituent in dendrobium nobile is extracted altogether, and extraction efficiency is higher, is conducive to the effect of giving full play to dendrobium nobile effective component.
Test example 2
Rat is randomly divided into normal group, model group, dendrobium nobile dosage group, Yang Weishu group according to weight, and every group 20；Model
Group, dendrobium nobile dosage group, Yang Weishu group give 167 μ g/mL MNNG and drink liquid (being fitted into 500mL black water bottle, freely drink),
Normal group freely drinks distilled water, and modeling 8 weeks, after histopathologic examination determines modeling success, dendrobium nobile dosage group filled daily
Stomach gives Herba Dendrobii extract produced by the present invention, dosage 1.34g/kg；Yangweishu granules are given in the daily stomach-filling of Yang Weishu group
4.0g/kg；Model group gives physiological saline stomach-filling, and normal group is not intervened, and after administration 12 weeks, carries out abdominal cavity fiber crops to 4 groups of rats
It is liquor-saturated, gastric tissue is taken out, fixes and be immersed in formalin saving.
Referring to gastrin GAS, Bai Jie in enzyme-linked immunosorbent assay (ELISA) detection kit detection mice serum
Plain IL-6 content.
Fig. 2 is embodiment 1, Herba Dendrobii extract made from embodiment 4~11 treats atrophic gastritis rat 12 weeks
Rat blood serum gastrin GAS, Interleukin-6 content, as result in figure it is found that dendrobium nobile made from normal group, the embodiment of the present invention
Extract doses group, the GAS content of Yang Weishu group are above model group；And dendrobium nobile made from normal group, the embodiment of the present invention mentions
Take the IL-6 content of object dosage group lower than model group；Compared with 4~11 experimental group rat of embodiment, by made from embodiment 1
After the treatment of Herba Dendrobii extract dosage group, gastrin GAS, Interleukin-6 content in rat blood serum contain in normal rabbit serum
Amount is close, illustrates that Herba Dendrobii extract made from embodiment 1 is best to the therapeutic effect of Chronic Atrophic Gastritis in Rat, and changes stone
Any condition in dry measure used in former times extract extracting method in step S3 can impact the extraction efficiency of Herba Dendrobii extract, thus
Herba Dendrobii extract is influenced to the therapeutic effect of Chronic Atrophic Gastritis in Rat.
Fig. 3 is embodiment 1, Herba Dendrobii extract made from embodiment 12~15 treats atrophic gastritis rat 12 weeks
Rat blood serum gastrin GAS, Interleukin-6 content, as result in figure it is found that dendrobium nobile made from normal group, the embodiment of the present invention
Extract doses group, the GAS content of Yang Weishu group are above model group；And dendrobium nobile made from normal group, the embodiment of the present invention mentions
Take the IL-6 content of object dosage group lower than model group；Compared with 12~15 experimental group rat of embodiment, by made from embodiment 1
After the treatment of Herba Dendrobii extract dosage group, gastrin GAS, Interleukin-6 content in rat blood serum contain in normal rabbit serum
Amount is close, illustrates that Herba Dendrobii extract made from embodiment 1 is best to the therapeutic effect of Chronic Atrophic Gastritis in Rat, and embodiment
Herba Dendrobii extract made from 13~14 is better than embodiment 12, embodiment 15 to the therapeutic effect of Chronic Atrophic Gastritis in Rat, says
Step S1 is freeze-dried temperature in bright change dendrobium nobile extracting method, can impact to the extraction efficiency of Herba Dendrobii extract, thus
Herba Dendrobii extract is influenced to the therapeutic effect of Chronic Atrophic Gastritis in Rat.
Fig. 4 is that Herba Dendrobii extract made from embodiment 14~29 treats 12 weeks rat blood serums to atrophic gastritis rat
Gastrin GAS, Interleukin-6 content, as result in figure it is found that Herba Dendrobii extract dosage group made from the embodiment of the present invention is equal
Higher than model group；And the IL-6 content of Herba Dendrobii extract dosage group made from the embodiment of the present invention is lower than model group；And embodiment 1
Herba Dendrobii extract obtained is better than embodiment 14~29 to the therapeutic effect of Chronic Atrophic Gastritis in Rat, illustrates that changing dendrobium nobile mentions
Reaction condition in step S4 is taken in method, the extraction efficiency of Herba Dendrobii extract can be impacted, to influence Herba Dendrobii extract
To the therapeutic effect of Chronic Atrophic Gastritis in Rat.
Fig. 5 is that Herba Dendrobii extract made from embodiment 30~35 treats 12 weeks rat blood serums to atrophic gastritis rat
Gastrin GAS, Interleukin-6 content, as result in figure it is found that Herba Dendrobii extract dosage group made from the embodiment of the present invention is equal
Higher than model group；And the IL-6 content of Herba Dendrobii extract dosage group made from the embodiment of the present invention is lower than model group；And embodiment 1
Herba Dendrobii extract obtained is better than embodiment 30~35 to the therapeutic effect of Chronic Atrophic Gastritis in Rat, illustrates that changing dendrobium nobile mentions
Reaction condition during step S5 simulated intestinal fluid extracts in method is taken, shadow can be caused to the extraction efficiency of Herba Dendrobii extract
It rings, to influence Herba Dendrobii extract to the therapeutic effect of Chronic Atrophic Gastritis in Rat.
The above, only of the invention illustrates embodiment, not to the present invention in any form with substantial limitation,
It should be pointed out that for those skilled in the art, under the premise of not departing from the method for the present invention, that makes several changes
It also should be regarded as protection scope of the present invention into supplement；All those skilled in the art, do not depart from spirit of that invention and
In the case where range, using the equivalent variations of a little change, modification and differentiation that disclosed above technology contents are made, it is
Equivalent embodiment of the invention；Meanwhile any equivalent variations that all substantial technologicals according to the present invention do above-described embodiment
Change, modification and differentiation, still fall within protection scope of the present invention.
Claims (10)
1. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory, which is characterized in that in parts by weight include following components: Herba Dendrobii extract 2~
20 parts, 5~15 parts of wetting agent, 5~10 parts of rubbing agent, 0.5~0.8 part of potassium sorbate, 0.5~1.0 part of thickener, dodecyl
2~5 parts of sodium sulphate, 0.2~0.5 part of sweetener, 5~15 parts of nanometer hydroxyapatite, 15~25 parts of deionized water.
2. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 1, which is characterized in that the Herba Dendrobii extract mentions
Method is taken, is included the following steps:
S1, by fresh dendrobium nobile, be put into low temperature drying case after cleaning and be freeze-dried；
S2, it the dendrobium nobile of freezing is poured into crusher is crushed；
S3, smashed dendrobe powder is taken, is extracted with simulate the gastric juice, extracting solution centrifugation；
S4, in the resulting filter residue of step S3 add disodium hydrogen phosphate-citric acid solution, regulation system pH value be 3.0~
5.0, the enzyme for destroying plant cell wall, mechanical shaking extraction is added；
S5, simulated intestinal fluid is dissolved in the resulting filter residue of step S4 extracts, extracting solution centrifugation；
S6, the supernatant liquid obtained after step S3~S5 centrifugation is merged, is concentrated under reduced pressure into the 1/3~1/5 of stoste volume；
S7, the dregs of a decoction for obtaining step S3~S6 merge, and drying crushes, dendrobium nobile dregs of a decoction powder is made after sieving.
3. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2, which is characterized in that in step S1, freeze-drying
Temperature is -30~-50 DEG C.
4. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2, which is characterized in that in step S3, according to 1g:10
Disodium hydrogen phosphate-citric acid solution is added as simulate the gastric juice extractant in the solid-liquid ratio of~50mL, and regulation system pH value is
2.0~3.0, it fullys shake uniformly, is filtered after 1~2h is extracted in 40~60 DEG C of water-baths.
5. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2, which is characterized in that in step S4, destroy plant
The enzyme dosage of cell wall is the 0.5~1% of filter residue quality, and concussion Extracting temperature is 35~40 DEG C, and the time is 1~2h.
6. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2 or 5, which is characterized in that the destruction plant is thin
The enzyme of cell wall is one of cellulase, zytase, pectase and 1,4 beta-glucanase or a variety of combinations.
7. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2, which is characterized in that in step S5, phosphoric acid is added
For disodium hydrogen-citric acid solution as simulated intestinal fluid extracting solution, regulation system pH value is 7.5~9.0, is mixed well, 40
1~2h of refluxing extraction at~60 DEG C.
8. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 2, which is characterized in that in step S7, drying temperature
It is 40 DEG C, smashes it through 200 meshes.
9. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 1, which is characterized in that the rubbing agent is dendrobium nobile medicine
Ground-slag, Radix Notoginseng powder, green tea powder are that 1:0.5~1:1~2 mix in mass ratio.
10. a kind of dendrobium nobile toothpaste of antibacterial anti-inflammatory according to claim 1, which is characterized in that the nano-hydroxy-apatite
Stone be partial size be 20~50nm nanometer hydroxyapatite, partial size be 50~80nm nanometer hydroxyapatite, partial size 80-
The nanometer hydroxyapatite of 200nm is the mixture of 1:1~3:1~3 by weight.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060054780A (en) * | 2004-11-16 | 2006-05-23 | 강영욱 | Material improving sanitation of oral cavity |
| CN103202884A (en) * | 2013-04-03 | 2013-07-17 | 西安交通大学 | Method for extracting hovenaia dulcis total alkaloid by semi-bionic-enzymic method |
| WO2015143783A1 (en) * | 2014-03-25 | 2015-10-01 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method therefor |
| CN105148034A (en) * | 2015-09-22 | 2015-12-16 | 浙江大学 | Preparing method for high-performance dendrobium officinale tea polyphenol compound |
| CN105832789A (en) * | 2016-05-16 | 2016-08-10 | 威海松龄诺可佳中药饮片股份有限公司 | Method for preparing saponin enriched product through high-pressure microwave assisted semi-bionic extraction |
| CN107028850A (en) * | 2017-04-24 | 2017-08-11 | 上海玉慈生物科技有限公司 | Dendrobium candidum toothpaste and its preparation technology with oral restoration function |
| CN108938524A (en) * | 2018-09-26 | 2018-12-07 | 安徽霍山黑石渡生物科技有限公司 | A kind of dendrobium candidum toothpaste and preparation method thereof |
-
2019
- 2019-04-24 CN CN201910332276.2A patent/CN110051596B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060054780A (en) * | 2004-11-16 | 2006-05-23 | 강영욱 | Material improving sanitation of oral cavity |
| CN103202884A (en) * | 2013-04-03 | 2013-07-17 | 西安交通大学 | Method for extracting hovenaia dulcis total alkaloid by semi-bionic-enzymic method |
| WO2015143783A1 (en) * | 2014-03-25 | 2015-10-01 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method therefor |
| CN105148034A (en) * | 2015-09-22 | 2015-12-16 | 浙江大学 | Preparing method for high-performance dendrobium officinale tea polyphenol compound |
| CN105832789A (en) * | 2016-05-16 | 2016-08-10 | 威海松龄诺可佳中药饮片股份有限公司 | Method for preparing saponin enriched product through high-pressure microwave assisted semi-bionic extraction |
| CN107028850A (en) * | 2017-04-24 | 2017-08-11 | 上海玉慈生物科技有限公司 | Dendrobium candidum toothpaste and its preparation technology with oral restoration function |
| CN108938524A (en) * | 2018-09-26 | 2018-12-07 | 安徽霍山黑石渡生物科技有限公司 | A kind of dendrobium candidum toothpaste and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 石奇: ""植物多糖的新型提取分离技术应用进展"", 《西安文理学院学报（自然科学版）》 * |
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