CN110025704B

CN110025704B - Compound Chinese herbal medicine preparation for treating piglet diarrhea and preparation method thereof

Info

Publication number: CN110025704B
Application number: CN201910449131.0A
Authority: CN
Inventors: 左瑞华; 蒋平; 孙桃桃; 黄仁术; 佘德勇; 夏伦斌; 刘莹莹; 卢道强; 查秀兰
Original assignee: West Anhui University
Current assignee: West Anhui University
Priority date: 2019-05-28
Filing date: 2019-05-28
Publication date: 2021-11-12
Anticipated expiration: 2039-05-28
Also published as: CN110025704A

Abstract

The invention discloses a compound Chinese herbal medicine preparation for treating piglet diarrhea, which comprises, by weight, 10-30 parts of codonopsis pilosula, 3-10 parts of astragalus membranaceus, 3-8 parts of radix bupleuri, 2-6 parts of poria cocos, 1-3 parts of andrographis paniculata, 1-5 parts of coptis chinensis, 0.5-4.5 parts of fried areca catechu, 0.5-2 parts of rheum officinale, 1-3 parts of fried bighead atractylodes rhizome, 1-4 parts of dried orange peel, 1-3 parts of gypsum, 1-4 parts of corydalis saxicola, 10-40 parts of dandelion, 1-15 parts of divaricate saposhnikovia root and 1-6 parts of honey-fried licorice root; the compound Chinese herbal medicine preparation has the effects of obviously improving the diarrhea symptom of piglets, improving the immunity of the piglets and the like, can replace partial chemical medicines to achieve the aim of treating the diarrhea of the piglets, has simple process, low requirement on equipment and easy implementation, is convenient to feed, has no toxicity, harm and residue, has good safety, and can meet the requirements of people on pollution-free livestock products.

Description

Compound Chinese herbal medicine preparation for treating piglet diarrhea and preparation method thereof

Technical Field

The invention relates to a compound Chinese herbal medicine preparation for treating piglet diarrhea and a preparation method thereof, belonging to the technical field of traditional Chinese medicines.

Background

The diarrhea of piglets is a typical multifactorial disease under intensive pig raising production conditions, is one of the most serious piglet disease groups at present and is also an important cause for piglet death, according to investigation, the piglet death due to the diarrhea accounts for 39.8% of the total number of the piglet death, the piglet diarrhea is in the first place in the harm of the pig raising industry, the healthy development of the pig raising industry is seriously threatened, the feed conversion rate is low, the survival rate of the piglet is reduced, the piglet grows slowly, the growth and development are stagnated, and even die, the piglet diarrhea is mainly treated by using antibiotics clinically at present, but the effect is not ideal, and the harm such as drug residue exists at the same time.

Disclosure of Invention

The invention mainly aims to provide a compound Chinese herbal medicine preparation for treating piglet diarrhea and a preparation method thereof, which have the effects of obviously improving piglet diarrhea symptoms, improving piglet immunity and the like, can replace partial chemical medicines, achieves the purpose of treating piglet diarrhea and can effectively solve the problems in the background technology.

In order to achieve the purpose, the invention adopts the technical scheme that:

a compound Chinese herbal medicine preparation for treating piglet diarrhea comprises, by weight, 10-30 parts of codonopsis pilosula, 3-10 parts of astragalus membranaceus, 3-8 parts of radix bupleuri, 2-6 parts of poria cocos, 1-3 parts of andrographis paniculata, 1-5 parts of coptis chinensis, 0.5-4.5 parts of fried areca catechu, 0.5-2 parts of rheum officinale, 1-3 parts of fried bighead atractylodes rhizome, 1-4 parts of dried orange peel, 1-3 parts of gypsum, 1-4 parts of corydalis saxicola, 10-40 parts of dandelion, 1-15 parts of divaricate saposhnikovia root and 1-6 parts of honey-fried licorice root.

The invention also provides a preparation method of the compound Chinese herbal medicine preparation for treating piglet diarrhea, which comprises the following steps:

(1) mixing and micronizing codonopsis pilosula, astragalus, poria cocos, fried areca seeds, fried bighead atractylodes rhizome, dried orange peel, divaricate saposhnikovia root, honey-fried licorice root and the like according to the parts by weight to obtain traditional Chinese medicine mixed superfine powder a;

(2) mixing and micronizing common andrographis herb, rhubarb, coptis chinensis, corydalis saxicola bunting and the like according to the parts by weight to obtain traditional Chinese medicine mixed superfine powder b;

(3) micronizing bupleuri radix, Gypsum Fibrosum, and herba Taraxaci separately;

(4) micronizing, respectively soaking the traditional Chinese medicine mixed superfine powder a, the radix bupleuri superfine powder and the dandelion superfine powder in 10 times of water for 1 hour;

(5) placing radix bupleuri superfine powder into a traditional Chinese medicine extraction tank, boiling, keeping the micro-boiling state for 0.5 hour, placing traditional Chinese medicine mixed superfine powder a into the traditional Chinese medicine extraction tank, boiling, keeping the micro-boiling state for 1.5 hours, placing dandelion superfine powder into the traditional Chinese medicine extraction tank, boiling, keeping the micro-boiling state for 1 hour, placing gypsum superfine powder into the traditional Chinese medicine extraction tank, boiling, and keeping the micro-boiling state for 2 hours;

(6) respectively discharging the liquid, filtering, adding 4 times of water into the dregs of a decoction, continuing decocting according to the steps, and combining the filtrates of the three times;

(7) placing the traditional Chinese medicine mixed superfine powder b into a traditional Chinese medicine extraction tank, extracting for 1 hour by using 20 times of 80% ethanol, wherein the extraction temperature is 80 ℃, discharging liquid, filtering, adding 15 times of 80% ethanol into the medicine residue, continuing extracting according to the steps, and combining the filtrates of the three times;

(8) after the extraction is finished, rotary evaporation is carried out to recover ethanol to obtain extract, and the obtained extract is dissolved by the traditional Chinese medicine mixed superfine powder a extracting solution;

(9) mixing all extractive solutions, concentrating at 50-60 deg.C until the concentration of crude drug is 0.5g/mL, bottling, and sterilizing to obtain Chinese herbal compound preparation.

The compound Chinese herbal medicine preparation has the effects of obviously improving the diarrhea symptom of piglets, improving the immunity of the piglets and the like, can replace partial chemical medicines to achieve the aim of treating the diarrhea of the piglets, has simple process, low requirement on equipment and easy implementation, is convenient to feed, has no toxicity, harm and residue, has good safety, and can meet the requirements of people on pollution-free livestock products.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

(3) micronizing bupleuri radix, Gypsum Fibrosum, and herba Taraxaci separately;

Example 1

A compound Chinese herbal medicine preparation for treating piglet diarrhea comprises the following components in parts by weight: 10 parts of codonopsis pilosula, 10 parts of astragalus membranaceus, 8 parts of radix bupleuri, 5 parts of poria cocos, 3 parts of andrographis paniculata, 2 parts of coptis chinensis, 4 parts of fried areca, 1 part of rheum officinale, 2 parts of fried bighead atractylodes rhizome, 3 parts of dried orange peel, 3 parts of gypsum, 2 parts of corydalis saxicola, 15 parts of dandelion, 7 parts of divaricate saposhnikovia root and 5 parts of honey-fried licorice root, and the compound preparation is prepared according to the preparation method.

Example 2

A compound Chinese herbal medicine preparation for treating piglet diarrhea comprises the following components in parts by weight: 15 parts of codonopsis pilosula, 5 parts of astragalus membranaceus, 5 parts of radix bupleuri, 6 parts of poria cocos, 2 parts of andrographis paniculata, 3 parts of coptis chinensis, 3 parts of fried areca, 2 parts of rheum officinale, 3 parts of fried bighead atractylodes rhizome, 1 part of dried orange peel, 2 parts of gypsum, 3 parts of corydalis saxicola, 20 parts of dandelion, 10 parts of divaricate saposhnikovia root and 4 parts of honey-fried licorice root, and the compound preparation is prepared according to the preparation method.

Example 3

A compound Chinese herbal medicine preparation for treating piglet diarrhea comprises the following components in parts by weight: 12 parts of codonopsis pilosula, 12 parts of astragalus membranaceus, 8 parts of radix bupleuri, 4 parts of poria cocos, 1 part of andrographis paniculata, 5 parts of coptis chinensis, 4.5 parts of fried areca, 0.5 part of rheum officinale, 1.5 parts of fried bighead atractylodes rhizome, 2 parts of dried orange peel, 1 part of gypsum, 4 parts of corydalis saxicola bunting, 10 parts of dandelion, 5 parts of divaricate saposhnikovia root and 3 parts of honey-fried licorice root, and the compound preparation is prepared according to the preparation method.

Selecting 40 diarrhea piglets of 40 days old, and averagely dividing into 4 groups, namely an example 1 group, an example 2 group, an example 3 group and a control group, wherein each group comprises 10 piglets; the diarrhea piglets in the groups of example 1, example 2 and example 3 are respectively added with the compound Chinese herbal medicine preparations in the examples 1, 2 and 3 into drinking water according to the dose of 5g crude drugs per pig, and the drinking water is administrated once a day in the morning and once a day for 7 days; the diarrhea piglets of the control group are fed with florfenicol powder; after the administration, weighing, collecting jugular venous blood by a non-anticoagulation tube, centrifuging, taking serum, and detecting liver function (AST, ALT) and kidney function (CREA, BUN) according to the requirements of a full-automatic biochemical kit; IgG level was detected according to the detection method of IgGELISA kit.

Before the test, sick piglets are listened, bow back, unwilling to walk, anorexia, frequent micturition of thin feces with mucus, feces polluted around anus, and serious piglets show sunken eye sockets and dehydration; after the test, the physical weakness of the piglets is obviously improved, the appetite is enhanced, the feed intake is obviously increased, and the condition of discharging the thin excrement is basically relieved; the diarrhea cure rate of the control group is 6, and 4 diarrhea cure rates are obviously relieved; example 1 group 9 healed, 1 healed substantially; example 2 group 8 healed, 2 significantly remitted; example 3 groups had 8 heals, 1 substantial heals and 1 significant relief; therefore, the compound Chinese herbal medicine preparation has better clinical curative effect on treating the diarrhea of the piglets.

TABLE 1 Effect of Compound herbal preparation on serum IgG level of diarrhea piglets (g/L)

Note: the same column of different letters indicates significant difference (P <0.05) and the same letters indicate insignificant difference (P > 0.05).

As can be seen from table 1, at the end of the experiment, the serum IgG levels of the weak piglets in the groups of example 1, example 2 and example 3 were significantly different (P <0.05) from those in the control group, wherein the serum IgG antibody levels of the groups of example 1, example 2 and example 3 were the highest but the difference was not significant (P >0.05) from those of the other groups 2, indicating that the compound herbal preparation has the effect of increasing the serum IgG antibody level.

TABLE 2 influence of compound Chinese herbal medicine preparation on liver and kidney functions of diarrhea piglets

As can be seen from Table 2, at the end of the test, the indexes of liver function (AST, ALT) and kidney function (CREA, BUN) of the weak piglets in the groups of example 1, example 2 and example 3 are higher than those of the control group, and the difference is significant (P is less than 0.05), which indicates that the compound Chinese herbal medicine preparation has the index of reducing the liver and kidney functions of the diarrhea piglets, and achieves the effect of protecting the liver and the kidney.

TABLE 3 Effect of Compound Chinese herbal medicine preparation on serum protein level of diarrhea piglets

As can be seen from Table 3, the serum total protein and albumin levels of the groups of example 1, example 2 and example 3 were higher than those of the control group after the test, but were not significantly different from those of the control group (P > 0.05); the immunoglobulin levels in the example 1, example 2, and example 3 groups were significantly higher than the control group (P <0.05), where; the globulin level of the group in the example 2 is highest and is remarkably higher than that of the group in the example 1 (P <0.05), but the difference is not remarkable compared with that of the group in the example 3 (P >0.05), and the compound Chinese herbal medicine preparation has the effect of increasing the serum immunoglobulin of the diarrhea piglets.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.

Claims

1. A compound Chinese herbal medicine preparation for treating piglet diarrhea is characterized in that: the composite material is prepared from the following raw materials in parts by weight: 10-30 parts of codonopsis pilosula, 3-10 parts of astragalus membranaceus, 3-8 parts of radix bupleuri, 2-6 parts of poria cocos, 1-3 parts of andrographis paniculata, 1-5 parts of coptis chinensis, 0.5-4.5 parts of fried areca, 0.5-2 parts of rheum officinale, 1-3 parts of fried bighead atractylodes rhizome, 1-4 parts of dried orange peel, 1-3 parts of gypsum, 1-4 parts of corydalis saxicola, 10-40 parts of dandelion, 1-15 parts of radix sileris and 1-6 parts of honey-fried licorice root;

the preparation method of the compound Chinese herbal medicine preparation for treating piglet diarrhea comprises the following steps:

(1) mixing and micronizing radix Codonopsis, radix astragali, Poria, semen Arecae, Atractylodis rhizoma, pericarpium Citri Tangerinae, radix Saposhnikoviae and radix Glycyrrhizae Preparata according to the above weight parts to obtain traditional Chinese medicine mixed superfine powder a;

(2) mixing and micronizing common andrographis herb, rhubarb, coptis chinensis and corydalis saxicola bunting according to the parts by weight to obtain traditional Chinese medicine mixed superfine powder b;

(3) micronizing bupleuri radix, Gypsum Fibrosum, and herba Taraxaci separately;

(9) mixing all extractive solutions, concentrating at 50-60 deg.C until the concentration of crude drug is 0.5g/mL, bottling, and sterilizing to obtain compound Chinese herbal preparation.