CN110004196A - Stalk-maize multiple product alcohol fuel and high-quality DDGS technique - Google Patents
Stalk-maize multiple product alcohol fuel and high-quality DDGS technique Download PDFInfo
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- CN110004196A CN110004196A CN201910285646.1A CN201910285646A CN110004196A CN 110004196 A CN110004196 A CN 110004196A CN 201910285646 A CN201910285646 A CN 201910285646A CN 110004196 A CN110004196 A CN 110004196A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention discloses a kind of stalk-maize multiple product alcohol fuel and high-quality DDGS techniques.The technique combines first generation Fuel Ethanol and second generation Fuel Ethanol, it is 1:3~4 by the mass ratio of control stalk and corn flour, stalk hydrolyzate is mixed with liquefied corn, adjust the optimal pH range of pH to fermentation strain, fermentation strain is added, it ferments, obtains the DDGS of alcohol fuel and high-quality.A coproduction generation of the present invention and two generation ethyl alcohol, while product quality is improved containing the xylo-oligosaccharide of high nutritive value in the DDGS produced, there is market potential application prospect.
Description
Technical field
The invention belongs to lignocellulosic hydrolysis technology field, it is related to a kind of stalk-maize multiple product alcohol fuel and height
The technique of the vinasse albumen feed (DDGS) of quality.
Background technique
Biomass energy is to solve reality and one of future source of energy crisis and the most potential approach of environmental pollution.Based on shallow lake
There is the problem of " striving grain with people, strive ground with grain " in the first generation Fuel Ethanol that powder and carbohydrate fermentation produce alcohol fuel, be not inconsistent
Close the strategy of sustainable development.The a large amount of stalk biomass energy of the residue generated in agricultural production is mainly incinerated, utilization rate
It is extremely low.Cellulosic ethanol technology not only can use waste crop straws but also can generate a kind of renewable and clean energy resource --- second
Alcohol has more advantage than first generation Fuel Ethanol.New generation is had become by the second generation Fuel Ethanol of raw material of cellulose
One of the mainstream of discipline new energy, but due to the limitation of the prematurity of key technology and economy, cellulosic ethanol technology is also
It can not carry out large-scale commercial application.
The problem of current cellulosic ethanol technology is primarily present includes: (1) lignocellulosic ethyl alcohol production at present intensity
It is generally relatively low with concentration of alcohol, only 0.3~0.5gL-1·h-1.And the production intensity of corn alcohol can reach 1.6-
2.0g·L-1·h-1, it is nearly 4 times of lignocellulosic ethyl alcohol production intensity.This means that the ethyl alcohol of production unit quality, wood
The cost of equipment of matter cellulose process is 4 times of corn technique, while energy consumption, the human cost etc. of production unit quality ethyl alcohol
It is much higher than corn alcohol technique；(2) lignocellulosic ethyl alcohol generates high added value byproduct DDGS unlike corn alcohol,
But individually corn alcohol technology can not produce the high-quality DDGS that can be rich in xylo-oligosaccharide.
Summary of the invention
The purpose of the present invention is to provide a kind of stalk-maize multiple product alcohol fuel and high-quality DDGS techniques.It is described
Technique combines first generation Fuel Ethanol and the second Fuel Ethanol, improves ethyl alcohol and produces intensity, while producing
Containing the xylo-oligosaccharide of high value in DDGS, product income is improved while reducing production cost.
Realize that the technical solution of the object of the invention is as follows:
Stalk-maize multiple product alcohol fuel and high-quality DDGS technique, the specific steps are as follows:
Step 1, smashed stalk is subjected to acid pretreatment, is dried to obtain pretreated stalk；
Step 2, hydrolase and water are added in pretreated stalk, adjusting pH to the optimal pH range of enzyme is hydrolyzed,
Centrifugation obtains stalk hydrolyzate；
Step 3, α-amylase alpha-amylase is added in corn flour, adjusts pH to 4.0~7.0, liquefies, obtain corn
Liquefier；
Step 4, it is 1:3~4 by the mass ratio of stalk and corn flour, stalk hydrolyzate is mixed with liquefied corn, adjusts
The optimal pH range of pH to fermentation strain is saved, fermentation strain is added, ferments, obtains the DDGS of alcohol fuel and high-quality.
The present invention, in step 1, the stalk is selected from wheat stalk, corn stover, agriculture forestry waste, rice straw, sorghum
The one or more of stalk, soybean stalk, forest trimmings.
The present invention, in step 1, the pretreatment can be dilute acid pretreatment, alkalescence pretreatment, hot-water pretreatment or steaming
Broken pretreatment of steam explosion etc..
The present invention, in step 2, the hydrolase uses hydrolase commonly used in the art, is selected from cellulase, wood
The mixture of one or more of dextranase and trichoderma reesei etc..
The present invention, in step 2, the pH is the optimal pH range of enzyme, generally 3.0~10.0.
The present invention, in step 3, the condensing temperature is 85 DEG C.
The present invention, in step 4, the fermentation strain uses alcohol fermentation bacterial strain commonly used in the art, is selected from ferment
Female, bacterium or mould etc..
Compared with current technology, the invention has the following advantages that
(1) corn and stalk coproduction improve the production intensity of ethyl alcohol, save the time required for digesting, and it is raw to improve ethyl alcohol
Efficiency is produced, man power and material needed for reducing production substantially reduces cost.
(2) mortifier generated in preprocessing process of the present invention is less, significantly improves enzymatic hydrolysis and fermentation efficiency.
(3) present invention process not only significantly promotes the production intensity of ethyl alcohol relative to lignocellulosic ethyl alcohol technology, and
And the high-quality DDGS rich in high value xylo-oligosaccharide can also be produced.
Detailed description of the invention
Fig. 1 is that dilute acid pretreatment stalk hydrolyzate and liquefied corn press different proportion mixed fermentation result figure, corn stalk
The ratio of stalk hydrolyzate and liquefied corn is 1:2 (A), 1:3 (B), 1:4 (C), 0:1 (D).
Fig. 2 is corn stalk hydrolysis and liquefied corn ratio is the content detection of xylo-oligosaccharide in DDGS obtained by 1:4
Result figure.
Specific embodiment
Below with reference to embodiment and attached drawing, the invention will be further described.
Embodiment 1
The influence to product DDGS quality is mixed according to the stalk hydrolyzate and liquefied corn of different proportion
1. obtaining stalk hydrolyzate.By 10% stalk (wt/wt), 1% dilute sulfuric acid (wt/wt) is put into 2L reaction kettle
In, reaction kettle is increased to 160 DEG C, cools down after maintaining 10min, obtained product is placed into baking oven (60 DEG C) and is dried to moisture
Content is between 10%-20%.The stalk that dilute acid pretreatment is crossed is put into conical flask, by 22% dry (wt/wt) plus fiber
The ratio of plain enzyme and zytase, cellulase and zytase is 7:3, adjusts ph4.0-7.0, is put into 50 DEG C,
250rpm shaking table is digested, and after the completion of enzymatic hydrolysis, solid-liquid is separated with centrifuge, and taking the supernatant liquor after being centrifuged is stalk
Hydrolyzate.
2. the acquisition of liquefied corn.The liquefied corn that concentration of substrate is 30% is configured, sterile water is taken to be put into conical flask
In, alpha-amylase and corn flour is added, after shaking up, adjusts ph to 5.7, is placed in water-bath, after keeping the temperature 4h at 85 DEG C, obtains beautiful
Rice liquefier.
3. mixed fermentation.Liquefied corn is mixed with the hydrolyzate of stalk by different proportion.By liquefied corn and straw
Stalk hydrolyzate is mixed according to the ratio of 1:2,1:3,1:4 and 0:1, is accessed yeast, is put into 30 DEG C, is sent out in 150rpm shaking table
Ferment.Product fuel ethyl alcohol and DDGS are obtained after fermentation.
The detection of 4.DDGS.Fermentation is obtained into mixture distillation, four kinds of different DDGS is obtained, is then baked to, lead to
It crosses and compares its color, it is found that the quality of DDGS when stalk hydrolyzate and liquefied corn ratio are 1:3 and 1:4 is apparently higher than other
Two kinds.
Study influence of the different proportion of stalk hydrolyzate and liquefied corn for fermentation.Stalk progress diluted acid is located in advance
Reason carries out the mixed proportion of fermenting experiment with hydrolyzate and liquefied corn mixing.Experimental result as shown in figure 1 and table 1, owns
Mixed proportion under, fermentation in 72h complete (Fig. 1).As the ratio 1:4 of corn stalk hydrolysis and liquefied corn,
Final concentration of alcohol has reached 118.80g/L (Fig. 2).When the ratio 1:2 of corn stalk hydrolysis and liquefied corn, finally
Concentration of alcohol is 104.89g/L.The production intensity of ethyl alcohol is different under different proportion as can be seen from Table 1, passes through corn
The method that Fuel Ethanol is combined with lignocellulosic Fuel Ethanol mentions for lignocellulosic ethyl alcohol technology
The high production intensity of ethyl alcohol, and according to the quality colors of the obtained DDGS of different proportion as shown in Fig. 2, wherein 1:3 with
The obtained DDGS color of 1:4 is more conform with national standard.The method of this mixed fermentation relative to Corn Fuel Ethanol technology,
Rich in xylo-oligosaccharide, the quality of DDGS is improved.
1 dilute acid pretreatment stalk hydrolyzate of table and data when liquefied corn mixed fermentation 96h
In conclusion the corn stover of dilute acid pretreatment and the mixture of corn have excellent fermenting property, ethyl alcohol production
Rate reached 1.94g/L/h or more at 48 hours.Compared with individual dilute acid pretreatment corn stover, corn and dilute acid pretreatment are beautiful
The mixture of rice stalk obtains preferably fermentation performance.Fig. 2 is produced by the stalk hydrolyzate of different proportion with liquefied corn
Raw DDGS.Stalk hydrolyzate and liquefied corn ratio are that the obtained DDGS quality of 1:3 and 1:4 is higher, wherein stalk water
Xylo-oligosaccharide content is 10859.32mg/kg, total phosphorus content in generated DDGS when being 1:4 for solution liquid and liquefied corn ratio
0.62%, coarse ash 12.5%, other equal indexs of crude protein 19.3% meet national standard, illustrate present invention process production
DDGS is a high-quality DDGS qualified and with xylo-oligosaccharide.
Claims (7)
1. stalk-maize multiple product alcohol fuel and high-quality DDGS technique, which is characterized in that specific step is as follows:
Step 1, smashed stalk is subjected to acid pretreatment, is dried to obtain pretreated stalk；
Step 2, hydrolase and water are added in pretreated stalk, adjusts pH to the optimal pH range of enzyme, is hydrolyzed, is centrifuged
Obtain stalk hydrolyzate；
Step 3, α-amylase alpha-amylase is added in corn flour, adjusts pH to 4.0~7.0, liquefies, obtains corn liquefaction
Liquid；
Step 4, it is 1:3~4 by the mass ratio of stalk and corn flour, stalk hydrolyzate is mixed with liquefied corn, adjusts pH
To the optimal pH range of fermentation strain, fermentation strain is added, ferments, obtains the DDGS of alcohol fuel and high-quality.
2. technique according to claim 1, which is characterized in that in step 1, the stalk is selected from wheat stalk, corn
The one or more of stalk, agriculture forestry waste, rice straw, broomcorn straw, soybean stalk, forest trimmings.
3. technique according to claim 1, which is characterized in that in step 1, the pretreatment be selected from dilute acid pretreatment,
Alkalescence pretreatment, hot-water pretreatment or steam blasting pretreatment.
4. technique according to claim 1, which is characterized in that in step 2, the hydrolase is selected from cellulase, wood
The mixture of one or more of dextranase and trichoderma reesei etc..
5. technique according to claim 1, which is characterized in that in step 2, the pH is 3.0~10.0.
6. technique according to claim 1, which is characterized in that in step 3, the condensing temperature is 85 DEG C.
7. technique according to claim 1, which is characterized in that in step 4, the fermentation strain is selected from yeast, bacterium
Or mould.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910285646.1A CN110004196B (en) | 2019-04-10 | 2019-04-10 | Process for co-producing fuel ethanol and DDGS by using straw and corn |
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| CN201910285646.1A CN110004196B (en) | 2019-04-10 | 2019-04-10 | Process for co-producing fuel ethanol and DDGS by using straw and corn |
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| CN110004196B CN110004196B (en) | 2023-05-05 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468161A (en) * | 2019-08-08 | 2019-11-19 | 国投生物科技投资有限公司 | The application of corn and rice in wheat alcohol fermentation processes and the method using mixed raw material progress raw material fermentation production ethyl alcohol |
| CN114807245A (en) * | 2021-01-18 | 2022-07-29 | 中国石油天然气股份有限公司 | Method for improving DDGS quality in corn ethanol process through secondary fermentation of vinasse |
| CN114794308A (en) * | 2021-01-18 | 2022-07-29 | 中国石油天然气股份有限公司 | Method for improving DDGS color in corn ethanol process based on vinasse pH regulation |
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2019
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468161A (en) * | 2019-08-08 | 2019-11-19 | 国投生物科技投资有限公司 | The application of corn and rice in wheat alcohol fermentation processes and the method using mixed raw material progress raw material fermentation production ethyl alcohol |
| CN110468161B (en) * | 2019-08-08 | 2021-05-04 | 国投生物科技投资有限公司 | Application of corn and rice in wheat ethanol fermentation process and method for producing ethanol by fermenting raw materials by using mixed raw materials |
| CN114807245A (en) * | 2021-01-18 | 2022-07-29 | 中国石油天然气股份有限公司 | Method for improving DDGS quality in corn ethanol process through secondary fermentation of vinasse |
| CN114794308A (en) * | 2021-01-18 | 2022-07-29 | 中国石油天然气股份有限公司 | Method for improving DDGS color in corn ethanol process based on vinasse pH regulation |
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