CN105755057A - Method for fermentative production of alcohol - Google Patents
Method for fermentative production of alcohol Download PDFInfo
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- CN105755057A CN105755057A CN201410788670.4A CN201410788670A CN105755057A CN 105755057 A CN105755057 A CN 105755057A CN 201410788670 A CN201410788670 A CN 201410788670A CN 105755057 A CN105755057 A CN 105755057A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The present invention relates to a method for fermentative production of alcohol, and the method is as follows: (1) crushing degermed corn to a particle size of 70-85%, and sieving by a 20 mesh sieve, and mixing with water and a clear solution to obtain powder slurry with dry matter concentration of 30-32 wt. %; (2) adding amylase into the powder slurry for liquefying to obtain liquefied mash; (3) adjusting the pH of the liquefied mash to 4.2-4.8, then inoculating saccharomycetes; and (4 ) adding an acidic protease, a yeast accelerator and glucoamylase into the liquefied mash inoculated with the saccharomycetes for fermentation, wherein the clear solution is a supernatant obtained by centrifugation of waste mash of the bottom of a crude tower in the edible alcohol production process. Ethanol content in a fermentation liquid obtained by the method can be as high as 15% or more by weight.
Description
Technical field
A kind of method that the present invention relates to fermenting and producing ethanol.
Background technology
Ethanol is the essential industry raw material being widely used in the every field such as food, medicine, chemical industry, scientific research.Due to the appearance of the energy shortage problem that impact and the world oil crisis of economic crisis bring, a kind of new forms of energy of ethanol instead oil, it is possible to the effective energy shortage problem alleviating current oil crisis.Along with the development of national economy, China's Alcohol Production achieves huge development, and gathering around spirituous manufacturing enterprise now constantly increases, and annual production is third place in the world, position, but the development facing challenges of alcohol industry and problem are also increasing more and more.
Much ethanol enterprise of current China so far or uses more traditional production technology, and the technique step of production lags far behind external advanced production level.The energy-conservation of Alcohol Production enterprise of China is traditional, needs aborning to add substantial amounts of soda acid to regulate pH value, and production efficiency is relatively low, relatively costly.
Therefore, the method being badly in need of now the higher fermenting and producing ethanol of a kind of productivity that can reduce cost and ethanol.
Summary of the invention
The invention aims to overcome the defect that in prior art, the productivity of ethanol is relatively low, relatively costly, it is provided that a kind of method of fermenting and producing ethanol.
The present inventor finds under study for action, adopt thick useless wine with dregs at the bottom of tower tower supernatant by centrifugation in edible ethanol production process that the maize raw material after sieving is sized mixing, the serosity obtained can be made to meet pH value requirement when adding amylase liquefaction, a large amount of soda acid can be saved and adjust the step of pH value, and adopt acid protease, yeast accelerator and saccharifying enzyme to carry out enzymatic liquefaction wine with dregs in yeast fermentation process, the content of ethanol in fermentation liquid can be improved, and reduce cost.
Therefore, to achieve these goals, a kind of method that the invention provides fermenting and producing ethanol, the method includes:
(1) by de-embryo crush maize, granularity is that 70-85% crosses 20 mesh sieves, is then configured to the powder slurry that dry concentration is 30-32 weight % with water and clear liquid;
(2) add amylase in starching to described powder to liquefy, obtain liquefied fermented glutinous rice;
(3) pH value regulating described liquefied fermented glutinous rice is 4.2-4.8, then inoculation yeast bacterium;
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, add acid protease, yeast accelerator and saccharifying enzyme to ferment;
Wherein, described clear liquid is useless wine with dregs at the bottom of thick tower tower supernatant by centrifugation in edible ethanol production process.
Useless wine with dregs at the bottom of tower tower thick in edible ethanol production process supernatant by centrifugation is used for preparing powder slurry by the present invention, can so that powder slurry need not add soda acid to regulate pH value before adding amylase, not only save cost, also reduce the long-term interpolation saccharomycetic suppression of acid-base pair, simultaneously, possibly together with the ion needed for yeast growth in this clear liquid, the addition of clear liquid can be conducive to the balance of yeast growth system, additionally, carry out culture propagation owing to the addition of acid protease, yeast accelerator and saccharifying enzyme simultaneously, be conducive to improving ethanol content.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Detailed description of the invention
Hereinafter the specific embodiment of the present invention is described in detail.It should be appreciated that detailed description of the invention described herein is merely to illustrate and explains the present invention, it is not limited to the present invention.
A kind of method that the invention provides fermenting and producing ethanol, the method includes:
(1) by de-embryo crush maize, granularity is that 70-85% crosses 20 mesh sieves, is then configured to the powder slurry that dry concentration is 30-32 weight % with water and clear liquid;
(2) add amylase in starching to described powder to liquefy, obtain liquefied fermented glutinous rice;
(3) pH value regulating described liquefied fermented glutinous rice is 4.2-4.8, then inoculation yeast bacterium;
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, add acid protease, yeast accelerator and saccharifying enzyme to ferment;
Wherein, described clear liquid is useless wine with dregs at the bottom of thick tower tower supernatant by centrifugation in edible ethanol production process.
According to method of the present invention, wherein, adopt said method to produce ethanol and can improve the ethanol content in prepared ethanol, but, in order to improve the ethanol content in fermentation liquid further, the temperature of described clear liquid is 80-85 DEG C, and dry substance concentration is 4-6 weight %.
In the present invention, in step (1), granularity be 70-85% cross 20 mesh sieves refer to de-embryo crush maize degree can be 70-85% de-embryo Semen Maydis can pass through 20 mesh sieves, the raw material then oversize and siftage starched together as furnishing powder.
According to method of the present invention, wherein, in step (1), the ratio of water and clear liquid is had no particular limits, for instance the weight ratio of water and clear liquid can be 1:0.3-0.5.
According to method of the present invention, wherein, in step (2), diastatic addition can be the consumption that this area is conventional, for instance, relative to 1 ton of de-embryo Semen Maydis, diastatic addition can be 0.07-0.09kg.
According to method of the present invention, wherein, in step (2), the condition of described liquefaction can be the liquefaction condition that this area is conventional, but, in order to improve the ethanol content in fermentation liquid further, preferably, the condition of described liquefaction includes: temperature is 80-85 DEG C, and the time is 2-3.5h.
According to method of the present invention, wherein, described acid protease can be the acid protease that this area is conventional, but, for instance can be the grand large protein enzyme in Shandong and/or Novi's letter protease.
In the present invention, yeast accelerator and saccharifying enzyme are the conventional yeast accelerator in this area and saccharifying enzyme.
According to method of the present invention, wherein, in step (3), the mode regulating pH value can be the mode regulating pH value of this area routine, for instance can be adopt sulfur acid for adjusting pH value.
In the present invention, yeast used can be the fermenting and producing ethanol yeast that this area is conventional, for instance can for the one in the super brewer yeast in Angel, Angel height brewer yeast, Angel SN yeast.
In the present invention, in step (3), saccharomycetic inoculum concentration is the 0.1-0.2% of liquefied fermented glutinous rice volume.
According to method of the present invention, wherein, in step (4), the consumption of acid protease, yeast accelerator and saccharifying enzyme can be the consumption that this area is conventional, but, in order to improve the ethanol content in fermentation liquid further, preferably, relative to 1 ton of de-embryo Semen Maydis, the consumption of described acid protease is 0.12-0.17kg, the consumption of described yeast accelerator is 0.002-0.005kg, and the consumption of described saccharifying enzyme is 0.85-1.1kg.
According to method of the present invention, wherein, in step (4), the condition of described fermentation can be the fermentation condition that this area is conventional, for instance may include that temperature is 32-34 DEG C, pH value is 4.6-5.2, and the time is 65-72h.
In the present invention, in step (4), to the addition sequence of acid protease, yeast accelerator and saccharifying enzyme, there is no particular limitation, it is possible to is simultaneously introduced, it is also possible to successively add.
It should be appreciated by those skilled in the art, edible ethanol production process is the edible ethanol production process that this area is conventional, and the preparation of the clear liquid in the present invention can for, after thick tower rectification terminates, taking supernatant after being centrifuged by the useless wine with dregs at the bottom of thick tower tower.Usually, the pH value of this supernatant is 3.6-3.8, and this clear liquid contains water, cellulose, residual starch, residual sugar, organic acid, ion.
Embodiment
By the useless wine with dregs at the bottom of thick tower tower respectively at 2200 rotating speeds, it is centrifuged under 2000 rotating speeds and 1800 rotating speeds, then supernatant is taken, preparing dry substance concentration respectively is 4 weight %, the clear liquid of 5 weight % and 6 weight %, wherein, the pH value of this clear liquid is 3.6, containing water, cellulose, residual starch, residual sugar, organic acid, ion in clear liquid.
Yeast accelerator is purchased from Angel Yeast Co., Ltd, and saccharifying enzyme believes China Investment Ltd. purchased from Novi, and yeast is purchased from Angel Yeast Co., Ltd.
Embodiment 1
The present embodiment method for the fermenting and producing ethanol of the present invention is described.
(1) by 1 ton of de-embryo crush maize, granularity is 70% mistake 20 mesh sieves, and then with water and 85 DEG C, the clear liquid (weight ratio 1:0.3) that dry substance concentration is 6 weight % is configured to powder slurry (pH value is 5.2) that dry concentration is 30 weight %;
(2) add 0.07kg Novi letter fire resistant alpha-diastase in starching to powder and at temperature 80 DEG C, carry out liquefaction 2h, obtaining liquefied fermented glutinous rice;
(3) pH value with the sulphuric acid adjustment liquefied fermented glutinous rice that concentration is 40% is 4.6, the then inoculation super brewer yeast bacterium in Angel (inoculum concentration is the 0.1% of liquefied fermented glutinous rice volume);
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, it is simultaneously introduced 0.12kg acid protease (Novi letter protease), 0.002kg yeast accelerator and 1.1kg saccharifying enzyme and is 32 DEG C in temperature, pH value is carry out fermentation 65h for 4.6 times, prepares fermentation liquid A1.
Embodiment 2
The present embodiment method for the fermenting and producing ethanol of the present invention is described.
(1) by 1 ton of de-embryo crush maize, granularity is 76% mistake 20 mesh sieves, and then with water and 85 DEG C, the clear liquid (weight ratio 1:0.4) that dry substance concentration is 4 weight % is configured to powder slurry (pH value is 5.4) that dry concentration is 31 weight %;
(2) add 0.08kg Novi letter fire resistant alpha-diastase in starching to powder and at temperature 82 DEG C, carry out liquefaction 3h, obtaining liquefied fermented glutinous rice;
(3) pH value with the sulphuric acid adjustment liquefied fermented glutinous rice that concentration is 40% is 4.6, the then inoculation super brewer yeast bacterium in Angel (inoculum concentration is the 0.2% of liquefied fermented glutinous rice volume);
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, it is simultaneously introduced 0.15kg acid protease (Novi letter protease), 0.005kg yeast accelerator and 0.9kg saccharifying enzyme and is 32 DEG C in temperature, pH value is carry out fermentation 68h for 5 times, prepares fermentation liquid A2.
Embodiment 3
The present embodiment method for the fermenting and producing ethanol of the present invention is described.
(1) by 1 ton of de-embryo crush maize, granularity is 85% mistake 20 mesh sieves, and then with water and 85 DEG C, the clear liquid (weight ratio 1:0.3) that dry substance concentration is 6 weight % is configured to powder slurry (pH value is 5.0) that dry concentration is 32 weight %;
(2) add 0.09kg Novi letter fire resistant alpha-diastase in starching to powder and at temperature 85 DEG C, carry out liquefaction 3.5h, obtaining liquefied fermented glutinous rice;
(3) pH value with the sulphuric acid adjustment liquefied fermented glutinous rice that concentration is 40% is 4.8, the then inoculation super brewer yeast bacterium in Angel (inoculum concentration is the 0.1% of liquefied fermented glutinous rice volume);
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, it is simultaneously introduced 0.17kg acid protease (Novi letter protease), 0.0035kg yeast accelerator and 0.85kg saccharifying enzyme and is 34 DEG C in temperature, pH value is carry out fermentation 72h for 5.2 times, prepares fermentation liquid A3.
Embodiment 4
The present embodiment method for the fermenting and producing ethanol of the present invention is described.
Ethanol is prepared according to the method for embodiment 3, the difference is that, acid protease used in step (4) is 0.10kg, prepares fermentation liquid A4.
Embodiment 5
Ethanol is prepared according to the method for embodiment 3, the difference is that, in step (1), the temperature of clear liquid is 90 DEG C, and dry substance concentration is 8 weight %, prepares fermentation liquid A5.
Comparative example 1
Ethanol is prepared according to the method for embodiment 1, the difference is that, by the water of the weight such as the clear liquid in step (1) changes into, prepare fermentation liquid D1.
Comparative example 2
Ethanol is prepared according to the method for embodiment 1, the difference is that, in step (4), it is added without acid protease, prepares fermentation liquid D2.
Test case
Adopting high performance liquid chromatograph (model is Agilent 1260) to measure the content of ethanol in the embodiment 1-5 fermentation liquid A1-A5 prepared and the comparative example 1-2 fermentation liquid D1-D2 prepared, measurement result is in Table 1.
Table 1
Embodiment | Ethanol content (weight %) in fermentation liquid |
Embodiment 1 | 16.3 |
Embodiment 2 | 16.4 |
Embodiment 3 | 16.5 |
Embodiment 4 | 15.2 |
Embodiment 5 | 15.1 |
Comparative example 1 | 13.0 |
Comparative example 2 | 12.5 |
Embodiment 1-5 and comparative example 1-2 is compared it can be seen that the content of ethanol can be high to more than 15 weight % in the fermentation liquid for preparing of the method for the present invention.
Embodiment 3 is compared it can be seen that when the consumption of acid protease is 0.12-0.17kg with embodiment 4, it is possible to improve the ethanol content in fermentation liquid further.
Embodiment 3 being compared with embodiment 5 it can be seen that the temperature of described clear liquid is 80-85 DEG C, dry substance concentration is 4-6 weight %, it is possible to improve the ethanol content in fermentation liquid further.
Useless wine with dregs at the bottom of tower tower thick in edible ethanol production process supernatant by centrifugation is used for preparing powder slurry by the present invention, can so that powder slurry need not add soda acid to regulate pH value before adding amylase, not only save cost, also reduce the long-term interpolation saccharomycetic suppression of acid-base pair, simultaneously, possibly together with the ion needed for yeast growth in this clear liquid, the addition of clear liquid can be conducive to the balance of yeast growth system, additionally, carry out culture propagation owing to the addition of acid protease, yeast accelerator and saccharifying enzyme simultaneously, be conducive to improving ethanol content.
The preferred embodiment of the present invention described in detail above; but, the present invention is not limited to the detail in above-mentioned embodiment, in the technology concept of the present invention; technical scheme can being carried out multiple simple variant, these simple variant belong to protection scope of the present invention.
It is further to note that, each concrete technical characteristic described in above-mentioned detailed description of the invention, in reconcilable situation, it is possible to be combined by any suitable mode, in order to avoid unnecessary repetition, various possible compound modes are no longer illustrated by the present invention separately.
Additionally, can also carry out combination in any between the various different embodiment of the present invention, as long as it is without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (7)
1. the method for a fermenting and producing ethanol, it is characterised in that the method includes:
(1) by de-embryo crush maize, granularity is that 70-85% crosses 20 mesh sieves, is then configured to the powder slurry that dry concentration is 30-32 weight % with water and clear liquid;
(2) add amylase in starching to described powder to liquefy, obtain liquefied fermented glutinous rice;
(3) pH value regulating described liquefied fermented glutinous rice is 4.2-4.8, then inoculation yeast bacterium;
(4) in the liquefied fermented glutinous rice after inoculation yeast bacterium, add acid protease, yeast accelerator and saccharifying enzyme to ferment;
Wherein, described clear liquid is useless wine with dregs at the bottom of thick tower tower supernatant by centrifugation in edible ethanol production process.
2. method according to claim 1, wherein, the temperature of described clear liquid is 80-85 DEG C, and dry substance concentration is 4-6 weight %.
3. method according to claim 1 and 2, wherein, in step (4), relative to 1 ton of de-embryo Semen Maydis, the consumption of described acid protease is 0.12-0.17kg, and the consumption of described yeast accelerator is 0.002-0.005kg, and the consumption of described saccharifying enzyme is 0.85-1.1kg.
4. method according to claim 1 and 2, wherein, in step (2), the condition of described liquefaction includes: temperature is 80-85 DEG C, and the time is 2-3.5h.
5. method according to claim 1 and 2, wherein, in step (3), regulates the mode of pH value for adopting sulfur acid for adjusting pH value.
6. method according to claim 1 and 2, wherein, in step (4), the condition of described fermentation includes: temperature is 32-34 DEG C, and pH value is 4.6-5.2, and the time is 65-72h.
7. method according to claim 1 and 2, wherein, in step (1), the weight of water and clear liquid is 1:0.3-0.5.
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CN108203721A (en) * | 2016-12-16 | 2018-06-26 | 湖北工业大学 | A kind of method that zymomonas mobilis utilizes corn producing and ethanol |
CN109266563A (en) * | 2018-09-28 | 2019-01-25 | 广西中粮生物质能源有限公司 | A kind of method that full Rice Production prepares distiller's yeast |
CN109576311A (en) * | 2018-11-14 | 2019-04-05 | 中粮生化能源(肇东)有限公司 | A kind of method and alcohol of fermenting and producing alcohol |
CN110004196A (en) * | 2019-04-10 | 2019-07-12 | 南京理工大学 | Stalk-maize multiple product alcohol fuel and high-quality DDGS technique |
CN110923269A (en) * | 2018-09-19 | 2020-03-27 | 南京理工大学 | Method for coproducing bioethanol from starchy material and fermented waste mash thereof |
CN111304261A (en) * | 2020-04-09 | 2020-06-19 | 吉林中粮生化有限公司 | Method for producing ethanol by fermentation |
CN111440828A (en) * | 2020-04-09 | 2020-07-24 | 吉林中粮生化有限公司 | Method for preparing slurry and liquefying starchy material and method for preparing ethanol |
CN113444749A (en) * | 2020-03-27 | 2021-09-28 | 中国石油天然气股份有限公司 | Preparation method of bioethanol |
CN114685247A (en) * | 2020-12-29 | 2022-07-01 | 国投生物科技投资有限公司 | Method and system for co-producing fuel ethanol and edible alcohol |
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CN108203721A (en) * | 2016-12-16 | 2018-06-26 | 湖北工业大学 | A kind of method that zymomonas mobilis utilizes corn producing and ethanol |
CN110923269B (en) * | 2018-09-19 | 2023-08-22 | 南京理工大学 | Method for co-producing bioethanol from starchy raw material and fermented waste mash thereof |
CN110923269A (en) * | 2018-09-19 | 2020-03-27 | 南京理工大学 | Method for coproducing bioethanol from starchy material and fermented waste mash thereof |
CN109266563A (en) * | 2018-09-28 | 2019-01-25 | 广西中粮生物质能源有限公司 | A kind of method that full Rice Production prepares distiller's yeast |
CN109576311A (en) * | 2018-11-14 | 2019-04-05 | 中粮生化能源(肇东)有限公司 | A kind of method and alcohol of fermenting and producing alcohol |
CN110004196B (en) * | 2019-04-10 | 2023-05-05 | 南京理工大学 | Process for co-producing fuel ethanol and DDGS by using straw and corn |
CN110004196A (en) * | 2019-04-10 | 2019-07-12 | 南京理工大学 | Stalk-maize multiple product alcohol fuel and high-quality DDGS technique |
CN113444749A (en) * | 2020-03-27 | 2021-09-28 | 中国石油天然气股份有限公司 | Preparation method of bioethanol |
CN111440828A (en) * | 2020-04-09 | 2020-07-24 | 吉林中粮生化有限公司 | Method for preparing slurry and liquefying starchy material and method for preparing ethanol |
CN111304261A (en) * | 2020-04-09 | 2020-06-19 | 吉林中粮生化有限公司 | Method for producing ethanol by fermentation |
CN111304261B (en) * | 2020-04-09 | 2023-08-08 | 吉林中粮生化有限公司 | Method for producing ethanol by fermentation |
CN114685247A (en) * | 2020-12-29 | 2022-07-01 | 国投生物科技投资有限公司 | Method and system for co-producing fuel ethanol and edible alcohol |
CN114685247B (en) * | 2020-12-29 | 2023-06-20 | 国投生物科技投资有限公司 | Method and system for co-producing fuel ethanol and edible alcohol |
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