CN109965138A

CN109965138A - A kind of functionality catfish feed eating belt agent and preparation method thereof

Info

Publication number: CN109965138A
Application number: CN201611217248.9A
Authority: CN
Inventors: 田岗; 邢晓慧
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-12-26
Filing date: 2016-12-26
Publication date: 2019-07-05

Abstract

The invention discloses a kind of functional catfish feed eating belt agents and preparation method thereof, belong to feed addictive preparation technical field, based on catfish nutritional need and water body environment, in conjunction with its characteristic of ingesting, optimizing raw material formula, science compounding is greatly improved palatability and efficiency of feed utilization, has compared with strong anti-oxidation effect, eliminates the composite flavor peptide of free radical；Cultures of S. cerevisiae with functional characteristics such as powerful trophism, bioactivity and inoxidizability；Calcium, iron and Soluble Fiber can be effectively supplemented, with powerful embedding effect, the calcium berry extract to extend the shelf life；Cryoprotective effects and stability are remarkably improved, water resistance and glossiness, enhancing and promotion palatability is improved, reduces the wormwood seed powder of the rate containing powder；It can provide the Wheat Sprout Powder of abundant nutrition substance, bioactive substance, fragrance matter and plant enzyme source；Finally prepare the functional catfish feed eating belt agent that a kind of palatability is strong, production performance is good.

Description

A kind of functionality catfish feed eating belt agent and preparation method thereof

Technical field

The present invention relates to feed addictives, and in particular to a kind of functionality catfish feed eating belt agent and preparation method thereof.

Background technique

The feeding behaviour of fish is a series of by search, positioning, receiving, acquisition etc. as other animal populations Quality of food evaluation procedure, then by the processes such as swallowing, digesting, absorbing and assimilating, this is directly related to energy requirement, again The existence of the growth, maturation and egg laying amount that decide fish squeezes out, and the feeding behaviour of fish is related to many sensory systems, still What is played a major role is two big sensory systems: olfactory system and gustatory system, and the feeding behaviour of fish can be divided into 2 stages: 1) perception of the olfactory system to the direction and position of food generates it close after interest；2) gustatory system is to the food sense of taste Evaluation, and finally decide whether to feed, in addition, various physical stimulation sense organs also play crucial work to the palatability of feed With.In short, smell and gustatory system feel to play main function to the food calling ingredient of fish in the feeding process of fish, exploitation with It can reasonably be played an important role to the growth of aquatic fish with protection water body environment using Feeding-attractants of Fish.

Catfish is animal feeding fish, is the excellent variety in fresh-water fishes, full of nutrition, aqueous in every 100 grams of flesh of fish Point 64.1 grams, 14.4 grams of protein, and contain several mineral materials and microelement, be particularly suitable for it is weak it is deficient, malnutritive it People is edible.In addition to the roe of catfish has miscellaneous taste inedibility, whole body is treasured, and catfish is rare nutrient excellent product, early in history records In just it is on the books, can compare favourably with shark's fin, wild soft-shelled turtle, be fish in treasure.Its dietary function and medical value is other Not available for fish, Qiang Jingzhuan bone and the effect of lengthening one's life are its unique features.Catfish is the good merchantable brand of stimulating milk secretion, property sweet in flavor simultaneously Temperature, and play the role of nourishing yin and nourishing blood, bowl spares gas, appetizing, diuresis is suitble to common people edible, especially with old, young, women's postpartum and The bad people of digestive function is applicable in the most, has a vast market foreground and development potentiality, becomes raiser's concern in recent years Hot spot.

Currently, patent disclosed in related catfish feed eating belt agent is more: 103053842 B of Chinese patent CN discloses one Kind catfish food calling nutritional agents, composition include fresh fish slurry, spiral shell freshwater mussel meat slurry, earthworm powder, yellow meal worm powder, spirulina powder, yeast powder, pig Liver xeraphium, EM bacterium, wheat-middlings, wheat-middlings are matrix, and various solid materials crush respectively, and trash fish is milled to fish slurry, and spiral shell freshwater mussel decladding takes Meat wears into meat slurry, and various raw material powder, slurry with the sieving of 100 mesh sieve tulles, are put into fodder mixing machine according to the proportion and stir 30- It 50 minutes, puddles uniformly, is stored after air-drying, when use is made an addition in catfish feed in 15% -25% ratio；The invention is matched The a variety of fresh animal raw materials of Fang Xuanyong, animal powder, spirulina, yeast and microorganism formulation, smell is unique, attractant is strong, right In the conversion feed of catfish, catfish tames food calling, and the feedstuff feeding for newly putting pond catfish in a suitable place to breed has good attractant effect, and also High-quality animal protein and various nutritional ingredients can be provided for catfish.

A kind of catfish bait of 104430233 A of Chinese patent CN and preparation method thereof.Specifically include: the bait is by Nian Fish meal, pluck, sucrose, sheep blood group merge by fermenting.Raw material used by the invention is easy to obtain, and valence Lattice are cheap, the bait prepared has one special fragrance, can be good at that catfish is attracted to take bait, then reach and rise to the bait Purpose.Meanwhile the prepared bait come out of the invention is at low cost, application effect is significant, is worth promoting.

104543450 A of Chinese patent CN discloses a kind of fermentation catfish feed, is made from the following raw materials in parts by weight: Soy noodle 80-90, sunflower meal 20-25, corn protein powder 6-8, red date powder 10-14, oyster meat 10-15, squid chip 5-10, bean curd Newborn 13-17, peach kernel 5-7, corn stem leaf 10-12, lemon 13-16, flower of Panax ginseng 1-2, palma 2-3, leek 2-3, yolk powder 7-9, butter 3-4, saccharomycete 2-3, phagostimulant 3-4, appropriate amount of water；The fermentation catfish feed of the invention, the oyster meat of addition, squid Piece is rich in multiple nutritional components such as the several mineral materials such as protein, calcium.The lemon of addition can play antibacterial, heat-clearing, promote appetite Effect.Various protein, fat, vitamin and mineral needed for catfish feed obtained is rich in catfish, proportion is suitable, accelerates The growth of catfish improves immunity of organisms and tissue metabolism's ability.

103704485 A of Chinese patent CN discloses a kind of clarias fuscus feed and preparation method thereof, by following parts by weight Raw material be made: ripe pig blood 45-50, minced fillet 12-15, fish meal 9-12, wheat bran 24-28, bean dregs 12-15, vinasse 5-6, rapeseed Cake 12-15, apple leaf 8-10, gynostemma pentaphylla 3-4, spermacoce latifolia 1-2, dandelion 2-3, twocolor sealavander herb 2-3, wolfberry leaf 4-5, rhizoma zingiberis 1-2, pueraria lobata 10-12, phagostimulant 3-4, feed fermentation agent 1-2.Contain phagostimulant in the invention feed formula, the food of fish can be improved It is intended to, increases appetite, quickly increase weight, meanwhile, the ingredients such as dandelion, gynostemma pentaphylla in formula can heat-clearing solution beneficial to human health Poison passes into human body by food chain, also beneficial to human health after clarias fuscus fishes are edible.

103598443 B of Chinese patent CN discloses a kind of catfish feed, the feed by following parts by weight raw material system : corn 55-60, soybean slag 20-25, dregs of beans 15-18, corn stover 12-15, black bean powder 10-12, wheat-middlings 12-15, water earthworm 6-8 parts of powder, sheep liver powder 5-6, shrimp shell meal 4-5, laver powder 5-6, dry ferment 4-5, it is dehydrated clover powder 6-8, asparagus lettuce leaf 6-8, food Appropriate salt, phagostimulant 4-5.The prepared catfish feed of the invention is added to water earthworm, sheep from the omnivorous characteristic of catfish The raw materials such as liver, seaweed, asparagus lettuce, nutritional ingredient needed for not only meeting the development of catfish fast-growth, while increasing feed Palatability avoids the waste of feed.

104082583 A of Chinese patent CN discloses a kind of catfish feed that duckweed/corn must be compound, and feature exists In being made from the following raw materials in parts by weight: black rice face 17-19, small rice and flour 18-20, sorghum flour 16-18, potato juice 11-13, Glucose 4-6, duckweed 17-21, corn must 12-14, dried small shrimp 13-15, quail meat 8-10, soya bean 14-16, sesame 4-8, Moschus 0.02-0.04, monosodium glutamate 0.1-0.2, grape seed oil 1.2-3.2, string of bells hung round the neck grass 0.1-0.3, Radix Isatidis 0.2-0.4, ginkgo leaf 0.2- 0.5, auxiliary agent 3-6, suitable quantity of water；Feed described in the invention is effectively compound by various ingredients, and nutrition is balanced, and addition duckweed, sweet tea are beautiful Rice must can excite the appetite of fish, have poly- fish fast, eat not scattered feature and easy to use long, easily storage has considerable Market application prospect.

Catfish phagostimulant disclosed above is although many kinds of, various informative, and ingredient is complicated, but flavor raw material does not have mostly It is extracted and is reprocessed again, wastage of material is serious, and phagostimulant additive amount is big, and flavor active principle utilization rate is low, and catfish The production performances such as palatability, digestibility, feedstuff-meat ratio, disease resistance it is still very unsatisfactory.

Therefore, it ingests the factors such as characteristic, digestion characteristics, nutritional need and water body environment, prepares a kind of agreeable to the taste in conjunction with catfish The functional catfish feed eating belt agent strong, that production performance is good of property is necessary.

Summary of the invention

Technical problem solved by the invention is to overcome the defect of existing catfish feed eating belt agent, with catfish nutritional need and Based on water body environment, in conjunction with its characteristic of ingesting, optimizing raw material formula, science compounding is greatly improved palatability and feed conversion Rate has compared with strong anti-oxidation effect, eliminates the composite flavor peptide of free radical；With powerful trophism, bioactivity and anti-oxidant The Cultures of S. cerevisiae of the functional characteristics such as property；Calcium, iron and Soluble Fiber can be effectively supplemented, there is powerful embedding effect, prolong The calcium berry extract of long shelf-life；Cryoprotective effects and stability are remarkably improved, water resistance and glossiness, enhancing and promotion are improved Palatability reduces the wormwood seed powder of the rate containing powder；It can provide abundant nutrition substance, bioactive substance, fragrance matter and plant enzyme The Wheat Sprout Powder in source；Finally prepare the functional catfish feed eating belt agent that a kind of palatability is strong, production performance is good.

In order to achieve the above object, the invention adopts the following technical scheme:

A kind of functionality catfish feed eating belt agent, is mainly prepared by the raw material of following parts by weight:

100 parts of composite flavor peptide, 8-12 parts of calcium berry extract, 6-12 parts of Wheat Sprout Powder, 5-9 parts of glycine betaine, tangerine peel powder 4-6 Part, 2-6 parts of wormwood seed powder, 7-11 parts of yeast culture, 2-4 parts of seawood meal, 0.5-1.5 parts of EM bacteria powder；

Further, the composite flavor peptide is science compounding earthworm, sheep blood and soybean protein, through high-pressure pulse electric, The low temperature processing techniques such as complex enzyme subsection enzymolysis, ultrafiltration, segmentation reaction, freeze-drying and what is prepared have earthworm, sheep blood and big concurrently The composite flavor flavor peptide of beans flavor, is not only greatly improved the palatability and efficiency of feed utilization of catfish feed, and have compared with Strong antioxidant effect eliminates free radical；

Preferably, the preparation method of the composite flavor peptide includes the following steps: that 4-6:2-4:1-3 is weighed in mass ratio Fresh earthworm, sheep blood and soybean protein mix, rub to obtain mixture, and the solion of 2-5 times of its quality is added, and mix, and adjust PH value is 6-8, at room temperature in electric field strength 25-35kV/cm, burst length 300-500 μ s, pulse frequency 200-300Hz item High-pressure pulse electric is carried out under part handles 20-30min；Then the complex enzyme of mixture quality 0.04-0.08% is added, in 50- Then 60 DEG C of enzymatic hydrolysis 30-50min are cooled to 35-45 DEG C of enzymatic hydrolysis 40-60min, 90 DEG C of enzyme deactivation 10min and are down to room temperature, degerming Enzymolysis liquid is filtered to obtain, enzymolysis liquid passes through the ultrafiltration membrance filter of 1000Da and 5000Da, takes the permeate between two films, be concentrated under reduced pressure into Solid content is that 18-22% must digest concentrate；The reduced sugar of enzymatic hydrolysis concentrate quality 5-7% is added, successively in 112-118 DEG C reaction 30-50min and 135-145 DEG C of reaction 45-65min after；Reaction solution is passed through to the ultrafiltration membrane mistake of 1000Da and 5000Da Filter, takes the permeate between two films, and being concentrated under reduced pressure into solid content is that 26-30% must react concentrate；To reaction concentrate The middle sericin peptide taken that its quality 0.2-0.4% is added, mixes, and is freeze-dried up to composite flavor peptide.

Preferably, the solion is to contain sodium ion 17-22mg/L, zinc ion 15-20mg/L, potassium ion 12- The aqueous solution of 14mg/L, calcium ion 9-11mg/L and magnesium ion 6-10mg/L；

Preferably, the complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase by matter Measure mixing more uniform than 4-6:2-4:0.2-0.4:0.1-0.3；

Preferably, the reduced sugar is one or more of xylose, ribose, glucose, fructose, galactolipin；

It is highly preferred that the quality group of the reduced sugar becomes: xylose: ribose: glucose: fructose: galactolipin=3-5:2- 4:1-3:1-3:1-2。

Further, the calcium berry extract is with calcium, iron and the higher calcium stem end of dietary fiber content, leaf of GAIGUO and chrysanthemum Powder is raw material, through ultrasonic cleaning, biological enzyme enzymatic hydrolysis, microwave drying, extrusion, ultramicro grinding and be made；Its Soluble Fiber Cellulose content is high, bioactivity is strong, has important, positive effect fiber to beneficial flora in catfish body, can especially supplement calcium Iron, the shelf-life for extending product, compared with full diet fiber, functionality, bioactivity are more powerful；

Preferably, the preparation method of the calcium berry extract, include the following steps: by calcium stem end, leaf of GAIGUO and inulin by Mass ratio 5-9:3-6:1-3 is uniformly mixed, and is placed in supersonic wave cleaning machine and is cleaned 3-5min in 200W, 30KHz, drains, and is crushed To partial size 0.3-0.7mm, the water of 0.5-1.5 times of fragment quality is added, adjusting pH value is 5-7, and mixture quality 0.1- is added 0.3% biological enzyme, in 50-60 DEG C of enzymatic hydrolysis 10-15min；It is 40-60% that enzymolysis liquid, which is concentrated under reduced pressure into solid content, is put into Microwave dryer is allowed to moisture and reaches 4-6%, be then crushed to partial size in 2000W, 130-150 DEG C of progress intermittent dryings 0.4-0.6mm is added the sodium bicarbonate of crushed material quality 0.2-0.4%, uniformly mixes, and adjustment mixture moisture content is 12- 14%, room temperature, sealing and standing 2-4h, in 140-160 DEG C of screw speed 105-115r/min, temperature condition extrusion, then It pulverizes to partial size 0.1-0.3mm up to calcium berry extract；

The biological enzyme is cellulase, laccase, dextranase, seminase, pectase 2-4:1-3:1- in mass ratio 3:0.5-1.5:0.4-1 is uniformly mixed.

Further, the preparation method of the Wheat Sprout Powder, include the following steps: for wheat to be placed on temperature be 10-18 DEG C, 20-40min is impregnated in the soak that pH value is 6-8, soak contains the sodium bicarbonate that mass percent concentration is 0.1-0.3% With the compound enzyme of 0.2-0.4%, it then is warming up to 28-32 DEG C with the rate of 3-5 DEG C/min, continues to impregnate 3-5h, finally with 5-7 DEG C/rate of min is warming up to 50-60 DEG C of immersion 5-8min, entire soaking process is primary every 15-20min ventilation, ventilation pressure 0.13-0.15MPa, while in electric field strength 3-5kV/cm, burst length 100-300 μ s, the progress of pulse frequency 60-80Hz condition High-pressure pulse electric processing, until wheat water content content is 40-45%；Wheat after immersion drains, and carries out in growing floor dark Germination, dark germination temperature are kept for 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture content after germinateing is 5-8%, low-temperature grinding cross 60-80 mesh up to Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase 2-4:2-4:1-3 in mass ratio: 1-2 is uniformly mixed.

Further, the wormwood seed powder is to assist bigcatkin willow through high-pressure electrostatic, high-pressure pulse electric using wormwood seed as raw material It crushed after acid soak, refrigerating processing, be lyophilized and be made；

Preferably, the preparation method of the wormwood seed powder includes the following steps: by wormwood seed sabot, first in electric-field strength It spends 1-3kV/cm high-pressure electrostatic and handles 5-7min；Then the soaking at room temperature 2-5h in the salicylic acid solution that concentration is 3-5mg/L, together When carry out high-pressure pulse electric under the conditions of electric field strength 2-6kV/cm, burst length 50-100 μ s, pulse frequency 100-150Hz Processing；Rinse, drain, in 3-5 DEG C of standing 18-24h, then successively 1-3 DEG C of refrigeration 2-4d, -3--5 DEG C of freezing 1-3d, - 15--18 DEG C of freezing 10-15h is immediately placed at outdoor natural light according to being crushed immediately after 2-4h, is lyophilized, freeze-dried powder quality is added The sodium bicarbonate of 3-8% uniformly mixes, and crosses 80-100 mesh up to wormwood seed powder.

Further, the yeast culture is with saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016 It spread cultivation, fermented step by step to go out bacterium germination and prepared；

Preferably, the preparation method of the yeast culture includes the following steps: the saccharomyces cerevisiae after taking 1 ring to activate Tlj2016 slant strains are seeded in the 500mL triangular flask equipped with 100mL seed culture medium and cultivate, in 28~30 DEG C, shaking table 25~30h is cultivated under the conditions of 150~200r/min of revolving speed, obtains primary seed solution；Primary seed solution is pressed into 5~10% (v/v) Inoculum concentration, be seeded in the 500mL triangular flask equipped with 100mL fermentation medium and carry out fermented and cultured, fermentation time 25~ 30h, 28~30 DEG C of temperature, 150~200r/min of shaking speed obtains secondary seed solution；By secondary seed solution with 5~10% (v/v) inoculum concentration be linked into dress 3L fermentation medium 5L fermentor ferment at constant temperature, 28~30 DEG C of temperature, 4~6L/ of ventilation quantity Min, 150~200r/min of revolving speed, fermentation full adjustment fermentation liquid pH value are 6.0~6.5, when fermentation is to 25~30h, with every The additive amount for rising 25~30mmol of fermentation liquid disposably adds L-cysteine into fermentation liquid, continues fermentation 20-30h and obtains most Whole fermentation liquid；It is 18-26% that final fermentation liquid, which is concentrated under reduced pressure into solid content, is freeze-dried up to yeast culture；

GSH final concentration reaches 3268-3308mg/L in the final fermentation liquid；

Viable bacteria content in the yeast culture are as follows: 7 × 10¹²-9×10¹²cfu/g；

The quality group of the seed culture medium becomes: (NH₄)₂SO₄6g/L, glucose 35g/L, K₂HPO₄·3H₂O 3g/ L, KH₂PO₄0.5g/L, yeast powder 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0；

The quality group of the fermentation medium becomes: (NH₄)₂SO₄10g/L, molasses 150g/L, K₂HPO₄·3H₂O 8g/ L, KH₂PO₄0.5g/L, yeast powder 5g/L, corn pulp 10g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0.

The saccharomyces cerevisiae is specially saccharomyces cerevisiae tlj2016, and it is micro- which has been preserved in China on July 15th, 2016 Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.12789, preservation address: Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101；

1) the saccharomyces cerevisiae tlj2016, which has characteristics that, reaches 300g/L to the tolerance of glucose, be conducive to it GSH is produced under the conditions of high concentration glucose；2) reach 3308mg/L in 5L fermentation cylinder for fermentation production GSH final concentration；3) resistance to It by the ability extra-heavy of L-cysteine, remains to slowly grow under the effect of 5mmol/L L-cysteine, in 40mmol/L L- half GSH is still able to maintain under cystine effect largely to synthesize；4) salt resistance ability reaches 18%, is conducive to extend its application field.

Another object of the present invention is to provide the preparation method of above-mentioned functional catfish feed eating belt agent, includes the following steps: According to formula, first successively by EM bacteria powder, yeast culture, Wheat Sprout Powder, composite flavor peptide, seawood meal, wormwood seed powder, old Pi Fen, glycine betaine, calcium berry extract quality 20-30% be added V-type blending tank uniformly mix 10-20min, speed of agitator 20- Then 40r/min is added remaining calcium berry extract and uniformly mixes 20-40min, speed of agitator 40-60r/min, sterile filling, close It seals, pack up to functional catfish feed eating belt agent.

The utility model has the advantages that

Functionality catfish feed eating belt agent of the invention is based on catfish nutritional need and water body environment, in conjunction with its spy that ingests Property, optimizing raw material formula, science compounding is greatly improved palatability and efficiency of feed utilization, has compared with strong anti-oxidation effect, eliminates The composite flavor peptide of free radical；Saccharomyces cerevisiae culture with functional characteristics such as powerful trophism, bioactivity and inoxidizability Object；Calcium, iron and Soluble Fiber can be effectively supplemented, with powerful embedding effect, the calcium berry extract to extend the shelf life；It can be significant Cryoprotective effects and stability are improved, water resistance and glossiness, enhancing and promotion palatability is improved, reduces the wormwood seed of the rate containing powder Powder；It can provide the Wheat Sprout Powder of abundant nutrition substance, bioactive substance, fragrance matter and plant enzyme source；Finally prepare one kind The functional catfish feed eating belt agent that palatability is strong, production performance is good.1) feeding experiment shows: similarly to feed and domestication side Catfish is fed when method, same daily management, same fish pond condition and same native catfish fingerling and injected volume, together In the case of the additive amount of sample, the catfish feed palatability for adding the functional catfish feed eating belt agent preparation of the present invention is strong, adult fish rule Lattice gap is small, and neatly, and then catfish baits, to compete situation of looking for food slight, and the low death rate is only 3%, reduces by 75% than control group； Disease resistance is strong, and disease incidence is only 3%, reduces by 75% than control group；Digestibility is high, and tail increases weight by force, improves than control group 61.97%；Feed coefficient is low, and feedstuff-meat ratio is low, reduces by 39.47% than control group；Culture efficiency is significant, in the case of same injected volume Output increased 65.51%, greatly reduces aquaculture cost, has preferable practicability and advance.2) catfish feed preparation examination Test and show: the catfish Moisture in Feed content and water activity of the functional catfish feed eating belt agent preparation of the addition present invention reduce respectively 1.72% and 1.73%, easy infection miscellaneous bacteria, long shelf-life, stability is not good；Rate containing powder reduces by 83.3%, and efficiency of feed utilization is high, It loses small, is conducive to maintain excellent aquatic environment；Pulverization rate reduces by 80.88%, and feed caking property is high, and particle is neat, gloss Property it is strong, be not easy transport and storage process rub dusting, improve raw material availability, promote the palatability of feed；Hardness mentions High by 32.13%, water resistance and durability degree are strong, are conducive to catfish and look for food, and extension is looked for food the time, improve efficiency of feed utilization and digestion Rate；Gelatinization degree is high, improves 79.2%, convenient for catfish digestion, absorbs, improves the digestive utilization ratio of catfish significantly.Tool Body technique effect is shown in embodiment 7-11；Particular technique principle is as follows:

1. saccharomyces cerevisiae tlj2016 culture viable bacteria content of the present invention is up to 7 × 10¹²-9×10¹²Cfu/g, it is final to ferment GSH final concentration reaches 3268-3308mg/L in liquid, not only increases the protein level and enzymatic activity of catfish feed, Er Qieti The high bioactivity and inoxidizability of catfish feed, has greatly shown the functional characteristic of catfish feed eating belt agent, ferment of making wine Female tlj2016 except have common yeast it is probiotic in addition to characteristic also with the following functions: 1) tolerance of glucose is reached 300g/L produces GSH under the conditions of high concentration glucose conducive to it；2) reach in 5L fermentation cylinder for fermentation production GSH final concentration 3308mg/L；3) it is resistant to the ability extra-heavy of L-cysteine, remains to slowly grow under the effect of 5mmol/L L-cysteine, GSH is still able to maintain under the effect of 40mmol/L L-cysteine largely to synthesize；4) salt resistance ability reaches 18%；By its science compounding It can be further, (1) removes the poisonous substances such as free radical, peroxide, heavy metal and aflatoxin in catfish feed；(2) it participates in The transhipment of amino acid (glutaminase, cysteine and other neutral amino acids)；(3) it is conducive to the absorption of iron, the absorption of selenium, calcium It absorbs, glutathione can also make the peroxidating fatty acid in feed revert to normal fat when absorbing or after absorbing；(4) Gastrointestinal tract mucosa epithelium is protected, the damage because of inflammation, ischaemic, oxidation material etc. to intestinal mucosa is prevented；(5) it stores and provides It forms amino acid (especially cysteine)；(6) synthesis of protein and DNA is participated in；(7) it is used as reducing substances, is conducive to dimension Raw element E, ascorbic reduction, maintain sulfydryl enzymatic activity.

2. wormwood seed powder prepared by the present invention is to be prepared using the seed wormwood seed of halophytes wormwood as raw material, contain Dimethyl-β thiopropionate (DMPT) abundant, is the good methyl donor of catfish, promotes the Nutrition and Metabolism of catfish, is had excellent Food calling and growth promoting function, compounded with raw materials science such as composite flavor peptide, Wheat Sprout Powder, seawood meal, glycine betaine, tangerine peel powders, It is remarkably improved the palatability of catfish feed.Also, there is stronger caking property while abundant nutrition substance is provided, very Be suitble to preparation catfish pellet, be greatly improved pellet at graininess and integrality, the catfish feed water resistance of preparation Good, glossiness is strong, and rate containing powder is low, and efficiency of feed utilization is high；Wormwood cold resistance is strong simultaneously, and (wormwood seed) wormwood seed is rich in freeze proof egg It is white, it is handled through high-pressure electrostatic, high-pressure pulse electric auxiliary Induced by Salicylic Acid, low temperature are segmented Stress treatment and the organic knot of natural lighting It closes, so that active seed containing freeze proof matrix itself is under the stress and induction of outer boundary's environment, freeze proof matrix components are obtained Most comprehensive, the most abundant synthesis and accumulation, improve the content of antifreeze protein, and then improve nutrition and biology in catfish feed The cryoprotective effects and stability of active material, extend the shelf-life of feed.

3. calcium berry extract prepared by the present invention is with calcic, iron and the higher calcium stem end of dietary fiber content, leaf of GAIGUO With inulin be raw material, through ultrasonic cleaning, biological enzyme enzymatic hydrolysis, microwave drying, extrusion, ultramicro grinding and be made；It is soluble Content of cellulose is high, bioactivity is strong, has important, positive effect fiber to beneficial flora in catfish body, can especially mend The shelf-life filled calcium iron, extend feed, compared with full diet fiber, functionality, bioactivity are more powerful；Simultaneously through ultra micro After crushing, be added a certain amount of sodium bicarbonate, sealing and standing after adjusted moisture, can significantly increase dietary fiber puffed degree and The content of soluble dietary fiber improves the functionality of dietary fiber, and gained calcium berry extract thickening property is stronger, than single side The modified dietary fiber of method preparation improves 40-50%, and is not influenced by acid, alkali, salt, and the water resistance of feed is significantly improved； Soluble Fiber cellulose content is high, and the modified dietary fiber than single method preparation improves 20-40%, it is easier to by lactic acid bacteria benefit With raising lactic acid bacteria increases the type and quantity of probiotics flora in the growth and fertility of animal body enteron aisle, reduces animal Body gut pH improves animal body intestine microenvironment；Adsorption capacity is strong, and after modified, the specific surface area of cellulose increases, Network is abundant, adsorption capacity enhancing, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, inhibits animal Absorption of the body to them；Ion-exchange capacity enhancing, to metallic element, especially heavy metal element adsorption effect is stronger, effectively Prevent the weight of animals metal poisoning；The resident time for adjusting and maintaining intestinal flora, enhances the digestion and absorbability of enteron aisle, Improve immunity of organisms；Gastrointestinal peristalsis is effectively facilitated, the adverse reactions such as gasteremphraxis, abdominal distension are slowed down and eliminate；Powerful embedding effect Influence of environment (oxygen, temperature, the illumination, water activity etc.) factor to product quality can be prevented, product is further stabilized Bioactivity and stability extend the shelf-life of product.

4. composite flavor peptide prepared by the present invention is science compounding earthworm, sheep blood and soybean protein, through high-pressure pulse electric, The low temperature processing techniques such as complex enzyme subsection enzymolysis, ultrafiltration, segmentation reaction, freeze-drying and what is prepared have earthworm, sheep blood and big concurrently The composite flavor flavor peptide of beans flavor, taste compound is abundant, is particularly suitable for catfish taste, can not only improve the suitable of catfish feed Mouth property, but also the digestion and absorption of catfish feed nutrient can be promoted comprehensively, efficiency of feed utilization is improved, to subtract significantly The discharge rate of the nutriments such as organic matter, nitrogen phosphorus in few excreta, reduces pollution of the undigested material to water environment in feed. Whole process uses low temperature processing techniques, avoids albuminous degeneration and other bioactive substances and fragrance matter caused by due to high temperature Loss can not only prevent extraction process living contaminants using high-voltage pulse electric field technology, but also significantly loose sheep blood and earthworm Structure between albumen and tissue, increases the permeability of tissue, is conducive to the infiltration and contact of subsequent complex enzyme, improves enzymatic hydrolysis The recovery rate of efficiency and flavor peptides, while can also directly increase the dissolution of soluble flavor peptides, it is pre-processed relative to existing high temperature It is more efficient, more environmentally friendly；Using subsection enzymolysis and segmentation Maillard reaction, the yield of composite flavor peptide is further improved；Ion The science compounding of solution and complex enzyme further improves the activity of complex enzyme, improves enzymolysis efficiency, reduces enzyme preparation Usage amount improves the acquisition of composite flavor peptide to the maximum extent；Using freeze-drying, tool especially has been compounded to reaction solution science There is the sericin peptide taken of excellent cryoprotective effects, greatly reduces the oxidational losses and freezing loss of composite flavor peptide, improve compound wind The inoxidizability of gustin, the final palatability that one kind is made and is not only greatly improved catfish feed, improves efficiency of feed utilization, and With compared with strong anti-oxidation effect, the composite flavor peptide of free radical is eliminated.

It is effectively prevented 5. Wheat Sprout Powder prepared by the present invention carries out high-pressure pulse electric processing to the wheat of soaking process Soak pollution microbes avoid generation stink and penetrate into Wheat Sprout Powder product, meanwhile, it can effectively enhance cereal kind chrotoplast The relative permeability of wall and cell membrane, the water absorption rate for improving full cereal seed promote seed to sprout in advance, improve superoxide anion certainly By generation rate and atriphos (ATP) content of base, the activation of a variety of biological enzyme of stratification wheat seed early period and release Put, endosperm dissolution and functional nutrient ingredient, bioactive ingredients, antioxidant content synthesis, promote the breathing generation of seed The effect of thanking accelerates the enrichment process of nutrition and functional materials, bioactive ingredients, antioxidant content, when shortening enrichment Between, the content of nutrition and functional materials, bioactive ingredients, antioxidant content is improved, impregnate with segmentation, rapidly heat up, Biological enzymolysis technique organically combines, and can further degrade kind of hide fiber element and a hemicellulose structure, increases kind of a skin permeability, activation Various bioactive substance (endogenous enzymes etc.) vigor are most strong, and enriching quantity is bigger, and Soluble Fiber cellulose content increases with it, and are wheat Catfish feed in bud powder provides trophic factors, and better effect is significant；By segmentation immersion, high-voltage pulse electric field technology and biology Enzymatic hydrolysis organically combines, and shortens soaking time, improves germination percentage and germination uniformity；Wheat Sprout Powder can be kept hot to greatest extent Quick property content of material, especially antioxidant (glutathione, phospho phytate, vitamin C, polyphenol etc.), to the maximum extent Guarantee natural colored, the taste and flavor of Wheat Sprout Powder, while may also function as bactericidal effect；Especially moderately killed through high temperature, short time After embryo, in the case where not influencing the bioactivity of wheat malt seed, with the absorption of moisture, wheat embryo is suppressed but not shadow The growth of items enzyme and bioactive substance and the dissolution of endosperm are rung, bud growth is very short, and respiration is weak, and sprout damage is low, mentions The high yield and quality of Wheat Sprout Powder, significantly improves the functional characteristic and digestion characteristics of phagostimulant.

6. the preparation method simple process of catfish feed eating belt agent of the present invention, low for equipment requirements, there will be powerful embedding The calcium berry extract of effect is added in two times, is mixed, and can be embedded completely other nutrition and bioactive substance wherein, maximum limit Loss of the phagostimulant in catfish feed granulating, storage is reduced to degree, improves the bioactivity and quality of phagostimulant Stability effectively extends the shelf-life of phagostimulant.

It should be noted that present invention functionality catfish feed eating belt agent has the technical effect that each component technical characteristic and side Method feature mutually cooperates with, interact as a result, the superposition of not simple technical characteristic (component function), each component technology are special The effect that the combination and collaboration of sign generate has preferable considerably beyond the superposition of each monotechnics feature functionality and effect Advanced and practicability.

Specific embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Range, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this Under the premise of invention spirit and scope, to the various changes or change of material component and dosage progress in these embodiments It belongs to the scope of protection of the present invention.

The preparation of 1 raw material of embodiment

1. the preparation of yeast culture

The preparation method of the yeast culture includes the following steps: that the saccharomyces cerevisiae tlj2016 after taking 1 ring to activate is oblique Face strain is seeded in the 500mL triangular flask equipped with 100mL seed culture medium and cultivates, in 30 DEG C, shaking speed 150r/min item 28h is cultivated under part, obtains primary seed solution；The inoculum concentration that primary seed solution is pressed to 8% (v/v) is seeded to and ferments equipped with 100mL Fermented and cultured is carried out in the 500mL triangular flask of culture medium, fermentation time 28h, 30 DEG C of temperature, shaking speed 180r/min is obtained Secondary seed solution；Secondary seed solution is linked into the 5L fermentor constant temperature hair of dress 3L fermentation medium with 8% (v/v) inoculum concentration Ferment, 30 DEG C of temperature, ventilation quantity 5L/min, revolving speed 200r/min, fermentation full adjustment fermentation liquid pH value is 6.0~6.5, works as fermentation When to 30h, L-cysteine is added disposably into fermentation liquid with the additive amount of every liter of fermentation liquid 28mmol, continues the 25h that ferments Obtain final fermentation liquid；It is 22% that final fermentation liquid, which is concentrated under reduced pressure into solid content, is freeze-dried up to yeast culture；

GSH final concentration reaches 3308mg/L in the final fermentation liquid；

Viable bacteria content in the yeast culture are as follows: 9 × 10¹²cfu/g；

2. the preparation of composite flavor peptide

The preparation method of the composite flavor peptide includes the following steps: that 5:3:2 in mass ratio weighs fresh earthworm, sheep blood And soybean protein, it mixes, rub to obtain mixture, the solion of 4 times of its quality is added, mix, adjusting pH value is 7, in room temperature Under under the conditions of electric field strength 30kV/cm, 400 μ s of burst length, pulse frequency 250Hz carry out high-pressure pulse electric processing 25min；Then then the complex enzyme that mixture quality 0.06% is added is cooled to 40 DEG C of enzymatic hydrolysis in 55 DEG C of enzymatic hydrolysis 40min 50min, 90 DEG C of enzyme deactivation 10min are down to room temperature, and aseptic filtration obtains enzymolysis liquid, and enzymolysis liquid passes through the ultrafiltration of 1000Da and 5000Da Film filtering, takes the permeate between two films, and concentrate must be digested by being concentrated under reduced pressure into solid content as 20%；Enzymatic hydrolysis concentration is added The reduced sugar of liquid quality 6%, successively after 40min and 140 DEG C of reaction 55min of 115 DEG C of reactions；By reaction solution by 1000Da and The ultrafiltration membrance filter of 5000Da takes the permeate between two films, and concentrate must be reacted by being concentrated under reduced pressure into solid content as 28%； The sericin peptide taken of its quality 0.3% is added into reaction concentrate, mixes, is freeze-dried up to composite flavor peptide.

The solion is to contain sodium ion 20mg/L, zinc ion 18mg/L, potassium ion 13mg/L, calcium ion 10mg/L With the aqueous solution of magnesium ion 8mg/L；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase 5:3 in mass ratio: 0.3:0.2 is uniformly mixed；

The quality group of the reduced sugar becomes: xylose: ribose: glucose: fructose: galactolipin=4:3:2:2:1.5.

3. the preparation of calcium berry extract

The preparation method of the calcium berry extract includes the following steps: calcium stem end, leaf of GAIGUO and inulin in mass ratio 7: 5:2 is uniformly mixed, and is placed in supersonic wave cleaning machine and is cleaned 4min in 200W, 30KHz, drains, be crushed to partial size 0.5mm, is added The water that 1 times of fragment quality, adjusting pH value is 6, the biological enzyme of mixture quality 0.2% is added, in 55 DEG C of enzymatic hydrolysis 12min；Enzyme It is 50% that solution liquid, which is concentrated under reduced pressure into solid content, is put into microwave dryer in 2000W, 140 DEG C of progress intermittent dryings, is allowed to Moisture reaches 5%, is then crushed to partial size 0.5mm, and the sodium bicarbonate of crushed material quality 0.3% is added, uniformly mixes, adjustment is mixed Closing object moisture content is 13%, room temperature, sealing and standing 3h, in 150 DEG C of screw speed 110r/min, temperature condition extrusions, Then it pulverizes to partial size 0.2mm up to calcium berry extract；

The biological enzyme is cellulase, laccase, dextranase, seminase, pectase 3:2:2:1:0.7 in mass ratio Uniformly mixing.

4. the preparation of Wheat Sprout Powder

The preparation method of the Wheat Sprout Powder includes the following steps: for wheat to be placed on the leaching that temperature is 15 DEG C, pH value is 7 30min is impregnated in bubble liquid, for 0.2% sodium bicarbonate and 0.3% compound enzyme, then soak contains mass percent concentration 30 DEG C are warming up to the rate of 4 DEG C/min, continues to impregnate 4h, 55 DEG C of immersion 7min are finally warming up to the rate of 6 DEG C/min, it is whole A soaking process is primary every 18min ventilation, ventilation pressure 0.14MPa, while in electric field strength 4kV/cm, 200 μ of burst length S, pulse frequency 70Hz condition carry out high-pressure pulse electric processing, until wheat water content content is 42%；Wheat drip after immersion It is dry, dark germination is carried out in growing floor, dark germination temperature is kept for 22 DEG C, and the dark germination time is 22h, the wheat drying after germinateing It is 6% to moisture content, low-temperature grinding crosses 80 meshes up to Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase 3:3:2:1.5 in mass ratio equal Even mixing.

5. the preparation of wormwood seed powder

The preparation method of the wormwood seed powder includes the following steps: by wormwood seed sabot, first in electric field strength 2kV/cm High-pressure electrostatic handles 6min；Then the soaking at room temperature 4h in the salicylic acid solution that concentration is 4mg/L, while in electric field strength 4kV/ High-pressure pulse electric processing is carried out under the conditions of cm, 80 μ s of burst length, pulse frequency 120Hz；It rinses, drain, stood in 4 DEG C Then 21h is successively immediately placed at outdoor natural light and shines after 3h immediately in 2 DEG C of refrigeration 3d, -4 DEG C of freezing 2d, -16 DEG C of freezing 12h It is crushed, is lyophilized, the sodium bicarbonate of freeze-dried powder quality 5% is added, uniformly mixes, cross 80 meshes up to wormwood seed powder.

Yeast culture used in following example 2-6, composite flavor peptide, calcium berry extract, Wheat Sprout Powder, wormwood seed Powder is prepared by embodiment 1, and other raw materials are commercially available.

Embodiment 2

100 parts of composite flavor peptide, 10 parts of calcium berry extract, 8 parts of Wheat Sprout Powder, 7 parts of glycine betaine, 5 parts of tangerine peel powder, wormwood seed 4 parts of powder, 9 parts of yeast culture, 3 parts of seawood meal, 1 part of EM bacteria powder；

Preparation method includes the following steps: according to formula, first successively by EM bacteria powder, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 25% be added V-type blending tank Then uniformly mixing 15min, speed of agitator 30r/min is added remaining calcium berry extract and uniformly mixes 30min, speed of agitator 50r/min, sterile filling, sealing are packed up to functional catfish feed eating belt agent.

Embodiment 3

100 parts of composite flavor peptide, 8 parts of calcium berry extract, 6 parts of Wheat Sprout Powder, 5 parts of glycine betaine, 4 parts of tangerine peel powder, wormwood seed 2 parts of powder, 7 parts of yeast culture, 2 parts of seawood meal, 0.5 part of EM bacteria powder；

Preparation method includes the following steps: according to formula, first successively by EM bacteria powder, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 20% be added V-type blending tank Then uniformly mixing 10min, speed of agitator 20r/min is added remaining calcium berry extract and uniformly mixes 20min, speed of agitator 40r/min, sterile filling, sealing are packed up to functional catfish feed eating belt agent.

Embodiment 4

100 parts of composite flavor peptide, 12 parts of calcium berry extract, 12 parts of Wheat Sprout Powder, 9 parts of glycine betaine, 6 parts of tangerine peel powder, wormwood 6 parts of seed powder, 11 parts of yeast culture, 4 parts of seawood meal, 1.5 parts of EM bacteria powder；

Preparation method includes the following steps: according to formula, first successively by EM bacteria powder, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 30% be added V-type blending tank Then uniformly mixing 20min, speed of agitator 40r/min is added remaining calcium berry extract and uniformly mixes 40min, speed of agitator 60r/min, sterile filling, sealing are packed up to functional catfish feed eating belt agent.

Embodiment 5

100 parts of composite flavor peptide, 8 parts of calcium berry extract, 12 parts of Wheat Sprout Powder, 5 parts of glycine betaine, 6 parts of tangerine peel powder, wormwood seed 2 parts of powder, 11 parts of yeast culture, 2 parts of seawood meal, 1.5 parts of EM bacteria powder；

Preparation method includes the following steps: according to formula, first successively by EM bacteria powder, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 20% be added V-type blending tank Then uniformly mixing 20min, speed of agitator 20r/min is added remaining calcium berry extract and uniformly mixes 40min, speed of agitator 40r/min, sterile filling, sealing are packed up to functional catfish feed eating belt agent.

Embodiment 6

100 parts of composite flavor peptide, 12 parts of calcium berry extract, 6 parts of Wheat Sprout Powder, 9 parts of glycine betaine, 4 parts of tangerine peel powder, wormwood seed 6 parts of powder, 7 parts of yeast culture, 4 parts of seawood meal, 0.5 part of EM bacteria powder；

Preparation method includes the following steps: according to formula, first successively by EM bacteria powder, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 30% be added V-type blending tank Then uniformly mixing 10min, speed of agitator 40r/min is added remaining calcium berry extract and uniformly mixes 20min, speed of agitator 60r/min, sterile filling, sealing are packed up to functional catfish feed eating belt agent.

The culture efficiency test of the functional catfish feed eating belt agent of 7 present invention of embodiment

1. fingerling and its source: being purchased from the native catfish of certain aquaculture base；

2. testing the selection in fish pond: having selected two mouthfuls of fish ponds in certain production of catfish is respectively experimental group and control group, fish pond Area is 3 mu, and the cultivation depth of water is 2.0-2.5 meters.The cultivation abundant water in fish pond is tested, water quality is pure and fresh pollution-free.Fish pond is set Independent culvert system, water inlet gate-valve chamber set three banister slots, and outer groove installs coarse strainer, and inside groove installs conic net, rhone peace Fill anti-escape network.

3. the test period: 2015.3.15-2015.11.15

4. cultural method: test group and control group are all made of following cultural method:

(1) pond preparation

1. pond is leveled: including the dry pool, dredging and shining pond.

2. drug disinfection: 7 days before fingerling stocking, changing water with quick lime 100~130,000 gram/acre and splash disinfection of cleaning up the pond, it is desirable that It splashes uniformly, the pond dead angle Bu Liu.

3. pond fills the water: filling the water within 5 days before fingerling stocking, fill level is 1.2 meters before fingerling stocking, after fingerling stocking It is gradually filled to peak level again.

(2) fingerling stocking: test cultivation fingerling launches the date on March 15th, 2015, when selecting fingerling, it is desirable that fingerling body Mass color is sliding, without disability, and fingerling, which enters, embathes disinfection 3~5 minutes with 5% saline solution before pond.It is thrown to test group and control at random Group fish pond, input situation are shown in Table 1.

1 soil Nian input situation of table

Fish pond	Kind	Specification (g/ tail)	Total mantissa	Average mu mantissa	Mu amount (Kg)	Total amount (Kg)
							Test group	Native catfish	50-100	9000	3000	225	675
Control group	Native catfish	50-100	9060	3020	226.5	679.5

(3) it tames:, away from 60 centimetres of bottom of pond, being taken with asbestos shingle with a distance from 2.5 meters of pond bank in direction of sitting in the north facing the south in fishpond If 3~5 food platforms.The same day that fingerling enters pond starts domestication and feeds, and people issues sound before bait throwing in, keeps fish Production conditions anti- It penetrates, when sound pauses, granular bait for fish is fed with wisp near bait table.2 times a day, every time 30~40 minutes, each bait throwing in Interval 10 seconds or so, adheres to domestication 7~10 days, fingerling ingests granular bait for fish quantity from less to more, and the intensity of feeding is by weak in this way To strong transformation, until taming successfully.

(4) raise: test group is identical with control group fish pond inventory, adds this hair that mass ratio is 5% in feed respectively Functional catfish feed eating belt agent and commercially available phagostimulant, phagostimulant type, specification, date of manufacture prepared by bright embodiment 2 is homogeneous Together.It feeds 2 times, is respectively fed once with 9:00-10:00,15:00-16:00 daily.Bait, which should be thrown, after fish domestication is eating On platform.The feeding rate of fish is 2%~6%, and daily ration of feeding should flexibly be grasped depending on cultivation concrete condition, every time to eat in 1 hour Complete is degree." four is fixed " principle should be also followed when feeding.

(5) daily management

1. regulating and controlling water quality: the preceding pond water level of fingerling stocking filled new water every 5~7 days after fingerling stocking at 1.2 meters or so Once.Add 10~15 centimetres of water every time.The high temperature cultivation season water of 7-Septembers adds to 2.0~2.5 meters, and former commonly using EM Dew improvement water quality, water transparency are 30~40 centimetres.

2. patrolling the pool: patrolling the pool daily and should follow and early, middle and late patrol pool system.Check food situation, the variation of water of fish Deng finding the problem and take timely measure.Especially weather is sultry or has the case where floating head omen to open aerator in time, keeps It is more than 5 mg/litre of water dissolved oxygen.

3. fish disease prevention and cure: fish disease prevention and cure according to " put prevention first, prevent and treat combination " policy.Accomplish it is disease-free first prevent, cultivating Chlorine dioxide or lime disinfection water body is commonly used in period.

5. pilot project and result:

It observed in the entire test period, detect catfish incidence, death condition and note down, statistical experiment group and control Group fish pond disease incidence and the death rate；Statistical experiment group and control group yield when the pool out (mantissa, cabrage, gross weight, tail increase weight)； As a result such as table 2:

Table 2: native catfish feeding experiment result

The above test results show that: similarly to feed and acclimation method, same daily management, same fish pond condition With feed catfish when same native catfish fingerling and injected volume, in the case of same additive amount, add function of the present invention Property catfish feed eating belt agent preparation catfish feed palatability it is strong, adult fish specification gap is small, and neatly, and then catfish baits, competes Situation of looking for food is slight, and the low death rate is only 3%, reduces by 75% than control group；Disease resistance is strong, and disease incidence is only 3%, compares control group Reduce by 75%；Digestibility is high, and tail increases weight by force, improves 61.97% than control group；Feed coefficient is low, and feedstuff-meat ratio is low, compares control group Reduce by 39.47%；Culture efficiency is significant, and output increased 65.51%, greatly reduces aquaculture cost in the case of same injected volume, With preferable practicability and advance.

It should be understood that the functional catfish feed eating belt agent of 3-6 of embodiment of the present invention preparation equally has above-mentioned reality Test effect, it is little between each embodiment and with above-mentioned experiment effect otherness.

Embodiment 8 adds the catfish feed primary quality measure detection of phagostimulant preparation of the present invention

The functional catfish feed eating belt agent and commercially available same size, type and production date for taking the embodiment of the present invention 2 to prepare The phagostimulant of phase is added in catfish feed with the additive amount of mass ratio 5% uniformly to be mixed, pelletizes, drying, respectively as test group And control group detects moisture, water activity, pulverization rate, rate containing powder, hardness, paste according to national aquatic granulated feed relevant criterion The main indicators such as change degree, testing result such as table 3:

Table 3: catfish feed primary quality measure testing result

Project	Moisture (%)	Water activity	Pulverization rate (%)	Rate containing powder (%)	Hardness (g)	Gelatinization degree (%)
							Test group	5.7	0.283	1.3	0.3	8360.77	86
Control group	5.8	0.288	6.8	1.8	6327.65	48
							Difference	- 1.72%	- 1.73%	- 80.88%	- 83.3%	32.13%	79.2%

The above result shows that: compared with commercially available, add the catfish feed of the functional catfish feed eating belt agent preparation of the present invention Moisture content and water activity reduce by 1.72% and 1.73% respectively, not easy infection miscellaneous bacteria, long shelf-life, and stability is good；Containing powder Rate reduces by 83.3%, and efficiency of feed utilization is high, and loss is small, is conducive to maintain excellent aquatic environment；Pulverization rate reduces by 80.88%, Feed caking property is high, and particle is neat, and glossiness is strong, is not easy to improve raw material availability in transport and storage process friction dusting, Promote the palatability of feed；Hardness improves 32.13%, and water resistance and durability degree are strong, is conducive to catfish and looks for food, extension is looked for food Time improves efficiency of feed utilization and digestibility；Gelatinization degree is high, improves 79.2%, convenient for catfish digestion, absorbs, significantly Improve the digestive utilization ratio of catfish.

Saccharomyces cerevisiae tlj2016 fermentation produces GSH capacity experimental under the conditions of the high sugar of embodiment 9

(1) shaking flask culture

Taking one ring of tlj2016 slant strains, access is equipped with 150rpm in the 250mL shaking flask of 30mL Shake flask medium, and 30 DEG C Culture 30h obtains seed liquor；

The Shake flask medium quality group becomes: (NH4)₂SO₄6g/L, glucose 35g/L, K₂HPO4·3H₂O 3g/L, KH₂PO₄0.5g/L, yeast powder 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0；

(2) 5L fermentation tank culture

Seed liquor is pressed into 10% inoculum concentration, access is equipped in the fermentor of 3L fermentation medium, and 30 DEG C, ventilatory capacity 6L/ Min, tank press 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, when fermentation is to 30h, disposably add final concentration For the L-cysteine of 25mmol/L, total fermentation time 50h；

The fermentation medium quality group becomes: (NH₄)₂SO₄10g/L, glucose 100g/L, K₂HPO₄·3H₂O 8g/ L, KH₂PO₄0.5g/L, yeast powder 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO4 0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH6.0；

After fermentation, the content for measuring GSH in fermentation liquid is 3308mg/L.

The experiment of 10 saccharomyces cerevisiae tlj2016L- cysteine tolerance of embodiment

By starting strain and each ring of tlj2016 slant strains, it is respectively connected to the 250mL equipped with 30mL Shake flask medium 150rpm in shaking flask, 30 DEG C are cultivated, and when culture is to 12h, the half Guang ammonia of L- of different final concentrations is added into shaking flask Acid is further cultured for 10h, measures dry cell weight, as a result table 4,5；

Shake flask medium (g/L): (NH₄)₂SO₄6, glucose 20, K₂HPO₄·3H₂O 3、KH₂PO₄0.5, yeast powder 11, MnSO₄ 0.1、KCL 0.1、FeSO4 0.1、MgSO₄·7H₂O 0.1, pH6.0；

Table 4: starting strain L-cysteine tolerance

L-cysteine concentration mmol/L	0	5	10	15	20	40
							Starting strain dry weight g/L	22.6	15.7	10.2	4.3	2.2	0.8
GSH concentration (mg/L)	35.6	46.7	43.2	40.7	37.9	25.3

5 tlj2016L- cysteine tolerance of table

L-cysteine concentration mmol/L	0	5	10	15	20	40
							Tlj2016 dry weight g/L	25.7	28.5	23.6	21.2	20.6	18.7
GSH concentration (mg/L)	73.2	98.3	113.5	121.7	127.5	135.8

It can be seen that from the result of table 4,5 for starting strain, L-cysteine added in culture medium, cell stops life It is long, and start self-dissolving, cause GSH growth rate to reduce with the raising of L-cysteine concentration；And half Guang ammonia of low concentration L- Under acid, tlj2016 still is able to slowly grow, and with the raising of L-cysteine concentration, the dry cell weight of tlj2016 bacterial strain is slow Decline, and GSH concentration sustainable growth, this result are beneficial in GSH production process through addition half Guang of precusor amino acids-L- Propylhomoserin proposes the production for promoting GSH.

The experiment of 11 saccharomyces cerevisiae tlj2016 salt resistance ability of embodiment

Take tlj2016 bacterium solution 1mL inoculation strain in containing different NaCl concentrations (concentration gradients 0%, 2%, 5%, 10%, 15%, 18%) 10mL YPD fluid nutrient medium (pH=6.5), are placed at 30 DEG C and cultivate respectively for 24 hours, each processing 3 A repetition.It respectively takes 1ml sample bacterium solution to mix in 9ml physiological saline, prepares dilution solution, take 0.1ml dilution solid in YPD It is coated in body plate, it is flat that culture 36 hours (each dilution does 3 in parallel) record calculating is inverted in 30 DEG C of biochemical cultivation cases Bacterium number number on plate.It the results are shown in Table 6, it is known that the resistance to salinity of the bacterium is 18%, illustrates that saccharomyces cerevisiae tlj2016 not only can be with It survives in conventional environment, still there is vigor under high salt conditions, can be applied to the food processing with high salt such as soy sauce, pickled product Sugar is consumed in journey produces glutathione.

6 salt resistance ability of table detection (× 10⁷cfu/ml)

Claims

1. a kind of functionality catfish feed eating belt agent, is mainly prepared by the raw material of following parts by weight: 100 parts of composite flavor peptide, 8-12 parts of calcium berry extract, 6-12 parts of Wheat Sprout Powder, 5-9 parts of glycine betaine, 4-6 parts of tangerine peel powder, 2-6 parts of wormwood seed powder, yeast training Support object 7-11 parts, 2-4 parts of seawood meal, 0.5-1.5 parts of EM bacteria powder.

2. functionality catfish feed eating belt agent as described in claim 1, which is characterized in that the preparation side of the composite flavor peptide Method includes the following steps: that 4-6:2-4:1-3 in mass ratio weighs fresh earthworm, sheep blood and soybean protein, mixes, rubs mixed Material is closed, the solion of 2-5 times of its quality is added, is mixed, adjusting pH value is 6-8, at room temperature in electric field strength 25-35kV/ High-pressure pulse electric is carried out under the conditions of cm, burst length 300-500 μ s, pulse frequency 200-300Hz handles 20-30min；Then Then the complex enzyme that mixture quality 0.04-0.08% is added is cooled to 35-45 DEG C of enzymatic hydrolysis in 50-60 DEG C of enzymatic hydrolysis 30-50min 40-60min, 90 DEG C of enzyme deactivation 10min are down to room temperature, and aseptic filtration obtains enzymolysis liquid, and enzymolysis liquid passes through the super of 1000Da and 5000Da Membrane filtration takes the permeate between two films, and being concentrated under reduced pressure into solid content is that 18-22% must digest concentrate；Enzyme is added The reduced sugar for solving concentrate quality 5-7%, successively in 112-118 DEG C of reaction 30-50min and 135-145 DEG C of reaction 45-65min Afterwards；The ultrafiltration membrance filter that reaction solution is passed through to 1000Da and 5000Da, takes the permeate between two films, is concentrated under reduced pressure into solid content Content is that 26-30% must react concentrate；The sericin peptide taken of its quality 0.2-0.4% is added into reaction concentrate, mixes, freezing It is drying to obtain composite flavor peptide；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase 4-6:2-4 in mass ratio: 0.2-0.4:0.1-0.3 is uniformly mixed.

3. functionality catfish feed eating belt agent as claimed in claim 2, which is characterized in that the solion be containing sodium from Sub- 17-22mg/L, zinc ion 15-20mg/L, potassium ion 12-14mg/L, calcium ion 9-11mg/L and magnesium ion 6-10mg/L Aqueous solution.

4. functionality catfish feed eating belt agent as claimed in claim 2, which is characterized in that the quality of the reduced sugar forms Are as follows: xylose: ribose: glucose: fructose: galactolipin=3-5:2-4:1-3:1-3:1-2.

5. functionality catfish feed eating belt agent as described in claim 1, which is characterized in that the preparation side of the calcium berry extract Method includes the following steps: uniformly to mix calcium stem end, leaf of GAIGUO and inulin 5-9:3-6:1-3 in mass ratio, it is clear to be placed in ultrasonic wave 3-5min is cleaned in 200W, 30KHz in washing machine, is drained, partial size 0.3-0.7mm is crushed to, 0.5-1.5 times of fragment quality is added Water, adjusting pH value be 5-7, be added mixture quality 0.1-0.3% biological enzyme, in 50-60 DEG C of enzymatic hydrolysis 10-15min；Enzymatic hydrolysis It is 40-60% that liquid, which is concentrated under reduced pressure into solid content, is put into microwave dryer and carries out intermittent do in 2000W, 130-150 DEG C It is dry, it is allowed to moisture and reaches 4-6%, be then crushed to partial size 0.4-0.6mm, the bicarbonate of crushed material quality 0.2-0.4% is added Sodium uniformly mixes, and adjustment mixture moisture content is 12-14%, room temperature, sealing and standing 2-4h, in screw speed 105-115r/ 140-160 DEG C of min, temperature condition extrusion, then pulverize to partial size 0.1-0.3mm up to calcium berry extract；

The biological enzyme is cellulase, laccase, dextranase, seminase, pectase 2-4:1-3:1-3 in mass ratio: 0.5-1.5:0.4-1 is uniformly mixed.

6. functionality catfish feed eating belt agent as described in claim 1, which is characterized in that the preparation side of the Wheat Sprout Powder Method, includes the following steps: for wheat to be placed on that temperature is 10-18 DEG C, pH value is that 20-40min is impregnated in the soak of 6-8, impregnates Liquid contains the sodium bicarbonate that mass percent concentration is 0.1-0.3% and the compound enzyme of 0.2-0.4%, then with 3-5 DEG C/min Rate be warming up to 28-32 DEG C, continue impregnate 3-5h, 50-60 DEG C of immersion 5- is finally warming up to the rate of 5-7 DEG C/min 8min, entire soaking process is primary every 15-20min ventilation, ventilation pressure 0.13-0.15MPa, while in electric field strength 3- 5kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high-pressure pulse electric processing, until wheat water content Content is 40-45%；Wheat after immersion drains, and dark germination is carried out in growing floor, and dark germination temperature is kept for 20-24 DEG C, secretly Germinating time is 20-24h, and the wheat drying after germinateing to moisture content is 5-8%, and low-temperature grinding crosses 60-80 mesh to obtain the final product Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase 2-4:2-4:1-3:1-2 in mass ratio Uniformly mixing.

7. functionality catfish feed eating belt agent as described in claim 1, which is characterized in that the preparation side of the wormwood seed powder Method includes the following steps: wormwood seed sabot, handles 5-7min in electric field strength 1-3kV/cm high-pressure electrostatic first；Then exist Concentration is soaking at room temperature 2-5h in the salicylic acid solution of 3-5mg/L, while in electric field strength 2-6kV/cm, burst length 50-100 High-pressure pulse electric processing is carried out under the conditions of μ s, pulse frequency 100-150Hz；It rinses, drain, in 3-5 DEG C of standing 18-24h, so Outdoor natural light successively is immediately placed in 1-3 DEG C of refrigeration 2-4d, 1-3d, -15--18 DEG C of freezing 10-15h of -3--5 DEG C of freezings afterwards It is crushed, is lyophilized immediately according to after 2-4h, the sodium bicarbonate of freeze-dried powder quality 3-8% is added, uniformly mixes, crossing 80-100 mesh is Obtain wormwood seed powder.

8. functionality catfish feed eating belt agent as described in claim 1, which is characterized in that the preparation side of the yeast culture Method includes the following steps: the saccharomyces cerevisiae tlj2016 slant strains after taking 1 ring to activate, is seeded to equipped with 100m L seed culture It is cultivated in the 500m L triangular flask of base, 25~30h is cultivated under the conditions of 28~30 DEG C, 150~200r/min of shaking speed, is obtained Primary seed solution；The inoculum concentration that primary seed solution is pressed to 5~10% (v/v), is seeded to equipped with 100m L fermentation medium Carry out fermented and cultured in 500m L triangular flask, 25~30h of fermentation time, 28~30 DEG C of temperature, 150~200r/ of shaking speed Min obtains secondary seed solution；Secondary seed solution is linked into the 5L hair of dress 3L fermentation medium with 5~10% (v/v) inoculum concentrations Fermentation tank ferment at constant temperature, 28~30 DEG C of temperature, 4~6L/min of ventilation quantity, 150~200r/min of revolving speed, fermentation full adjustment fermentation Liquid pH value is 6.0~6.5, when fermentation is to 25~30h, with the additive amount of every liter of 25~30mmol of fermentation liquid into fermentation liquid one Secondary property adds L-cysteine, continues fermentation 20-30h and obtains final fermentation liquid；Final fermentation liquid is concentrated under reduced pressure into solid content and contains Amount is 18-26%, is freeze-dried up to yeast culture；

The saccharomyces cerevisiae tlj2016 deposit number is CGMCC No.12789.

9. functionality catfish feed eating belt agent as claimed in claim 8, which is characterized in that GSH is whole in the final fermentation liquid Concentration reaches 3268-3308mg/L；Viable bacteria content in the yeast culture are as follows: 7 × 10¹²-9×10¹²cfu/g。

10. the preparation method of functional catfish feed eating belt agent as described in claim 1-9 is any, which is characterized in that including such as Lower step: according to formula, first successively by EM bacteria powder, yeast culture, Wheat Sprout Powder, composite flavor peptide, seawood meal, wormwood Seed powder, tangerine peel powder, glycine betaine, calcium berry extract quality 20-30% be added V-type blending tank uniformly mix 10-20min, stir Then revolving speed 20-40r/min is added remaining calcium berry extract and uniformly mixes 20-40min, speed of agitator 40-60r/min, sterile It is filling, seal, pack up to functional catfish feed eating belt agent.