CN109964807A - A kind of breeding method of the black glutinous CMS line of Erect Panicle - Google Patents
A kind of breeding method of the black glutinous CMS line of Erect Panicle Download PDFInfo
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- CN109964807A CN109964807A CN201910246215.4A CN201910246215A CN109964807A CN 109964807 A CN109964807 A CN 109964807A CN 201910246215 A CN201910246215 A CN 201910246215A CN 109964807 A CN109964807 A CN 109964807A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The present invention provides a kind of breeding method of Erect Panicle black rice CMS line, by hybridization and back cross breeding method the channel genes of control erect head character into the black glutinous CMS line of hybrid rice, it is bred as the black glutinous CMS line with erect head character, the sterile line proper height, stem is sturdy, outcrossing seed-setting rate is high, and the hybrid seed yield of hybrid paddy rice can be improved with its preparation ternary hybrid rice, reduce production cost and reduce environmental pollution.The advantages that Hybrid Rice Combination of combo is resistance to fertile, anti-fall, economic characters are good, in good taste.
Description
Technical field
Present invention relates particularly to a kind of breeding methods of the black glutinous CMS line of Erect Panicle, belong to Special Rices breeding technique
Field.
Background technique
Rice is made to Food Security in China is solved with people's health level is improved as Chinese first generalized grain crop
Tremendous contribution.In recent years, with the rapid development of our country's economy, the continuous improvement of living standards of the people, urban and rural residents' diet knot
The change of structure, as long as requirement of the people to rice is Well fed,well bred. by the past, it is good to become to be now to eat, and eats health.Black Rice is
A kind skin is in the coloured rice of black, edible with brown rice, and compared with general rice, black rice has higher nutritive value,
The content of protein, amino acid and fatty acid is more abundant, also more rationally, and containing more minerals zinc needed by human,
The physiological activators such as iron, manganese, selenium, germanium, calcium, phosphorus and flavones, alkaloid, heart tonifying infantile malnutrition due to digestive disturbances or intestinalparasites, 3 one glucoside of cyanine, and it is abundant
Vitamin B1, B2, B3, D, E and niacin, there is certain medical value, also can be used as wine brewing, extract haematochrome and production is fragrant
The raw material of type beverage, therefore, the breeding of Black Rice have broad application prospects.Currently, Black Rice is wide in variety with farm variety
Based on, Hybrid Rice Varieties are less, and most black rice hybrid paddy rice Planting characteristics are poor, and plant is higher, anti-rice blast and energy resistant to lodging
Power is poor, and intolerant to fertilizer, setting percentage is low, and amylose content is high, and mouthfeel is poor.The rice panicle type group structure of erect head type is closed
Reason, the ecological conditions such as canopy intensity of illumination, temperature, humidity are superior, are conducive to improve fertilizer resistance anti-lodging, Erect Panicle sterile line is also
Be conducive to pollen transmission, improve outcrossing seed-setting rate.By the especially dominant erect head gene of erect head gene by hybridization, backcrossing
Method transformation into black glutinous CMS line good in taste, by combo can be bred as it is resistance to it is fertile, anti-fall, economic characters are good, quality
Excellent black rice hybrid paddy rice.
Summary of the invention
The purpose of the present invention is to provide a kind of selections of the black glutinous CMS line of Erect Panicle, are cultivated by assembling
Resistance to black rice hybrid paddy rice fertile, anti-fall, economic characters are good, best in quality out.
To achieve the above object, using following technical scheme:
A kind of selection of the black glutinous CMS line of Erect Panicle, comprising:
(1) it is that B makees maternal and black glutinous rice paternal hybrid that selection three, which is glutinous rice holding, and a hybrid seed generation and glutinous rice holding are B7-
The backcrossing of 8 generations, breeding skin containing black race and other characters are that glutinous rice keeps being the consistent black glutinous holding of B being B with three；
(2) it is B for female parent with the black glutinous holding that step (1) obtains, using Erect Panicle kind as male parent, hybridizes to obtain F1 generation seed；
F1 generation selfing, obtains F2 for seed；
(3) plantation F2 selects the rice plant bagging selfing that Erect Panicle, economical character are excellent, and listed mark for segregating population
Note, after mature, decladding is rough out, retains the brown rice with glutinous rice character；
(4) it selects economical character excellent in self progeny and is able to maintain the Erect Panicle of CMS line rice varieties characteristic
Black glutinous rice plant continuous multi-generation selfing is stablized, black glutinous three system of Erect Panicle that economical character is excellent keeps system；
(5) it keeps system for male parent with black glutinous three system of Erect Panicle described in (4), is surveyed in pairs with common black glutinous CMS line
It hands over；Sterile plant is chosen in test cross offspring, black glutinous three system keeps system's backcrossing with Erect Panicle；
(6) keep system for circulation male parent with black glutinous three system of Erect Panicle, taken in backcross progeny Erect Panicle black rice sterile plant with
Circulation male parent carries out continuous multi-generation backcrossing, obtains the black glutinous CMS line of Erect Panicle.
In breeding material of the present invention, " erect panicle variety " is erect head rice varieties 259, the erect head character
It is to be controlled by dominant gene.During cultivating black glutinous CMS line, used three be that glutinous rice keeps being B being " good agriculture
Wx2B ", black glutinous rice variety be " duck blood is glutinous ", black glutinous CMS line be " the black A of blood " (Zhang Juncai, Xiao Yeqing, Chen great Zhou etc., it is black
Rice three series mating preliminary study Agriculture in Jiangxi journal, 2000).
The above four strains are introduced by project Rice Research Institute, Fujian Academy of Agricultural Science where applicant, agriculture
Skill character is excellent.
Select the black A of blood, black glutinous B with upright 259 hybridize and be returned can breeding obtain the excellent Erect Panicle of economical character
Black glutinous three system keeps system and sterile line.
Before the black glutinous CMS line of breeding Erect Panicle, three systems for needing first acquired character stable keep system, straight in breeding
When vertical black glutinous three system of fringe type keeps being, the method combined using selfing and test cross, by for years, the selfing in mostly generation carries out
M8003 line, after Progeny plants are stablized, screening economical character is excellent and is able to maintain the fertile of CMS line sterile gene
The black glutinous rice strain of Erect Panicle, as holding system.
The hybrid for being able to maintain three lines paddy sterile line sterile gene and referring to plant Yu CMS line rice varieties
F1 is thorough abortion.
It includes proper height that the economical character is excellent, stem is sturdy, sword-like leave is shorter, big fringe.
When black glutinous three system of breeding Erect Panicle keeps being, it was preferably 8 generations that the total algebra being generally selfed, which was 7~8 generations,.
It is to be returned in selfing using holding to save the time in the black glutinous CMS line of breeding Erect Panicle
Method, that is, keeping system, single plant is listed since forth generation and hybridizes with sterile line, is returned (listed individual plant selfing), passed through for 6~7 generations
Backcrossing, i.e. the excellent black glutinous CMS line of Erect Panicle of acquisition economical character.
Beneficial effects of the present invention:
Effect of the invention is that by hybridization and back cross breeding method the channel genes of control erect head character to hybridizing water
In the black glutinous CMS line of rice, it is bred as the black glutinous CMS line with erect head character, the sterile line proper height, stem
It is sturdy, outcrossing seed-setting rate is high, preparing ternary hybrid rice with it can be improved the hybrid seed yield of hybrid paddy rice, reduces production cost and reduction
Environmental pollution.The advantages that Hybrid Rice Combination of combo is resistance to fertile, anti-fall, economic characters are good, in good taste.
Specific embodiment
Embodiment 1
(1) it is that B makees maternal and black glutinous rice paternal hybrid that selection three, which is glutinous rice holding, and a hybrid seed generation and glutinous rice holding are B7-
The backcrossing of 8 generations, breeding skin containing black race and other characters are that glutinous rice keeps being the consistent black glutinous holding of B being B with three；
(2) it is B for female parent with the black glutinous holding that step (1) obtains, using Erect Panicle kind as male parent, hybridizes to obtain F1 generation seed；
F1 generation selfing, obtains F2 for seed；
(3) plantation F2 selects the rice plant bagging selfing that Erect Panicle, economical character are excellent, and listed mark for segregating population
Note, after mature, decladding is rough out, retains the brown rice with glutinous rice character；
(4) it selects economical character excellent in self progeny and is able to maintain the Erect Panicle of CMS line rice varieties characteristic
Black glutinous rice plant continuous multi-generation selfing is stablized, black glutinous three system of Erect Panicle that economical character is excellent keeps system；
(5) it keeps system for male parent with black glutinous three system of Erect Panicle described in (4), is surveyed in pairs with common black glutinous CMS line
It hands over；Sterile plant is chosen in test cross offspring, black glutinous three system keeps system's backcrossing with Erect Panicle；
(6) keep system for circulation male parent with black glutinous three system of Erect Panicle, taken in backcross progeny Erect Panicle black rice sterile plant with
Circulation male parent carries out continuous multi-generation backcrossing, obtains the black glutinous CMS line of Erect Panicle.
Described three be that glutinous rice keeps being B being good agriculture wx2B；Erect Panicle kind is erect head rice varieties 259；Black glutinous rice
It is glutinous for duck blood；Black glutinous CMS line is the black A of blood.
Embodiment 2
Black glutinous three system of Erect Panicle keeps the breeding of system
1) selection three be glutinous rice keep be good agriculture wx2B make it is maternal with duck blood is glutinous hybridizes, the first generation of hybrid and good agriculture wx2B are returned, and are selected
Educate skin containing black race and other characters BC1F1 single plant similar with good agriculture wx2B；BC1F1 is planted, and praises agriculture wx2B backcrossing, warp again
After spending 8 generations of backcrossing, skin containing black race and other characters can be selected and consistent stablize of good agriculture wx2B black glutinous keeps being B.
2) it is B as male parent using the black glutinous holding selected, 50, first familiar generation seed is hybridized to obtain with erect head 259；
3) 36 plants of F1 plant of plantation hybridization, selfing mix after mature and receive F2；
4) 5000 plants of F2 segregating population are planted, erect head Characters Identification is carried out after neat fringe, and (after Qi Sui 15 days, measurement fringe neck is bent
Degree, using sword-like leave pulvinus to fringe, as the standard at fringe angle, fringe neck curvature is less than 40 degree for the line of point and the angle of stalk extended line
Erect head), it is excellent to harvest 158 economical characters for selection and the rice strain listing mark with erect head character, maturity period single plant sowing
The F3 seed of good single plant；
5) 4) gained F3 seed is shelled out rough, selects the black rice brown rice with glutinous rice character；
6) plantation 5) gained F3, therefrom selection simultaneously have erect head character, economical character it is excellent, with it is black it is glutinous holding be B most phase
As 72 plants of listing marks of single plant；
6) F4 is planted, selects economical character excellent and black 12 plants of the glutinous single plant of the Erect Panicle of thorough abortion；
7) after the plant that self progeny screens continuously was selfed for 4 generations, it is maternal progress test cross with the black A of blood, selects Erect Panicle, strain
It is high it is moderate, stem is sturdy, and be black glutinous holding based material as three with black glutinous material that the black A test cross of blood is thorough abortion, pass through
It is black glutinous holding system that stable three are achieved with after the selfing in continuous 8 generation, and is named as upright black glutinous B.
Embodiment 3
The breeding of the black glutinous CMS line of Erect Panicle:
1) the upright black glutinous B (three systems that i.e. embodiment 2 is bred as keep system) for planting F8, chooses 1 plant of best single plant, black with blood
A carries out pairs of test cross；
2) plantation step 1) in whole test cross plant, select black 5 plants of the glutinous sterile plant of excellent Erect Panicle, the Erect Panicle with F9
Black glutinous holding system backcrossing, obtains BC1F1 seed；
3) BC1F1 is planted, black 5 plants of the glutinous sterile plant of excellent Erect Panicle is selected, black glutinous holding system is returned with F10 Erect Panicle, obtains
BC2F1 seed；
4) BC2 is planted, pollen microscopic examination is carried out to sterile plant, strictly selects sterile plant, takes the erect head of 5 plants of fine individual plant with F11
The black glutinous holding system backcrossing of type, obtains BC3F1 seed；
5) step 4) is repeated, until obtaining BC5F1 seed, plants BC5F1, pollen microscopic examination is carried out to sterile plant, stringent selection is sterile
5 plants of strain, black glutinous holdings system is returned with F13 Erect Panicle, obtains BC6F1 seed, at the same with the purple fragrant test cross in the Fujian restorer F, preparation is miscellaneous
Hand over combination；
6) BC6F1 is planted, pollen microscopic examination is carried out to sterile plant, carries out quality, the identification of disease-resistant and coordinate force, the erect head with F14
The black glutinous holding system backcrossing of type, is bred as CMS line, names as upright black glutinous A.
It is worth noting that, three systems keep system to be still constantly selfed in the CMS line Breeding Process, it is true
On, which keeps system not occur trait segregation, i.e. three systems keep system without be continuously selfed can also, directly educated with above-mentioned
At three systems keep system be used as circulation male parent.
Sterile line proper height, the stem of above-mentioned steps breeding are sturdy, outcrossing seed-setting rate is high, prepare ternary hybrid rice with it
The hybrid seed yield of hybrid paddy rice can be improved, per hectare can achieve 3-4 tons, simultaneously as fringe neck is upright, reduce blade to fringe portion
It blocks, can not have to when the production of hybrid seeds cut leaf, save labor；920 demand is reduced, the pollution to environment is reduced.The hybridization of combo
The advantages that rice combination is resistance to fertile, anti-fall, economic characters are good, in good taste.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (2)
1. a kind of breeding method of the black glutinous CMS line of Erect Panicle, it is characterised in that: described method includes following steps:
(1) it is that B makees maternal and black glutinous rice paternal hybrid that selection three, which is glutinous rice holding, and a hybrid seed generation is that glutinous rice is kept with three
It is that B passes through 7-8 generation backcrossing, selects skin containing black race and other characters and three are that glutinous rice keeps being the consistent black glutinous holding of B being B；
(2) it is B for female parent with the black glutinous holding that step (1) obtains, using Erect Panicle kind as male parent, hybridizes to obtain F1 generation seed；
F1 generation selfing, obtains F2 for seed；
(3) plantation F2 selects the rice plant bagging selfing that Erect Panicle, economical character are excellent, and listed mark for segregating population
Note, after mature, decladding is rough out, retains the brown rice with glutinous rice character；
(4) it selects economical character excellent in self progeny and is able to maintain the Erect Panicle of CMS line rice varieties characteristic
The black continuous 6-7 generation selfing of glutinous rice plant is stablized, black glutinous three system of Erect Panicle that economical character is excellent keeps system；
(5) it keeps system for male parent with black glutinous three system of Erect Panicle described in (4), is surveyed in pairs with common black glutinous CMS line
It hands over；Sterile plant is chosen in test cross offspring, black glutinous three system keeps system's backcrossing with Erect Panicle；
(6) keep system for circulation male parent with black glutinous three system of Erect Panicle, taken in backcross progeny Erect Panicle black rice sterile plant with
Circulation male parent carries out continuous 6-7 generation backcrossing, obtains the black glutinous CMS line of Erect Panicle.
2. a kind of breeding method of the black glutinous CMS line of Erect Panicle according to claim 1, it is characterised in that: described
Three be glutinous rice holding be B be good agriculture wx2B；Erect Panicle kind is erect head rice varieties 259；Black glutinous rice is that duck blood is glutinous；It is black
Glutinous CMS line is the black A of blood.
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| CN201910246215.4A CN109964807B (en) | 2019-03-29 | 2019-03-29 | Method for cultivating upright ear type black glutinous rice three-line sterile line |
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| CN201910246215.4A CN109964807B (en) | 2019-03-29 | 2019-03-29 | Method for cultivating upright ear type black glutinous rice three-line sterile line |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782901A (en) * | 2014-01-06 | 2014-05-14 | 福建省农业科学院水稻研究所 | Method for breeding erected-wheatear indica type three-line sterile line |
| CN104488699A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of erect panicle, long-stigma and elongated upmost internode two-line male sterile lines |
| CN104488700A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104585019A (en) * | 2015-01-15 | 2015-05-06 | 福建农林大学 | Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line |
-
2019
- 2019-03-29 CN CN201910246215.4A patent/CN109964807B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103782901A (en) * | 2014-01-06 | 2014-05-14 | 福建省农业科学院水稻研究所 | Method for breeding erected-wheatear indica type three-line sterile line |
| CN104488699A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of erect panicle, long-stigma and elongated upmost internode two-line male sterile lines |
| CN104488700A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104585019A (en) * | 2015-01-15 | 2015-05-06 | 福建农林大学 | Breeding method of erect panicle type long stigma and elongated upmost internode three-line sterile line |
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