CN109954524A - A kind of micro-fluidic chip to be shone based on homogeneous chemistry - Google Patents
A kind of micro-fluidic chip to be shone based on homogeneous chemistry Download PDFInfo
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- CN109954524A CN109954524A CN201910220873.6A CN201910220873A CN109954524A CN 109954524 A CN109954524 A CN 109954524A CN 201910220873 A CN201910220873 A CN 201910220873A CN 109954524 A CN109954524 A CN 109954524A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0803—Disc shape
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Abstract
The present invention relates to electrochemiluminescent immunoassay detection technique field, especially a kind of micro-fluidic chip to be shone based on homogeneous chemistry；Including chip body, chip body is provided centrally with rotation card slot, the outside of rotation card slot is annularly provided with sample cavity, quantitative cavity, waste liquid cavity and dilution cavity, quantitative cavity, mixing cavity is provided on the outside of waste liquid cavity and dilution cavity, it mixes and is provided with pipeline two on the outside of cavity, the inside of pipeline two is additionally provided with several reagent cavitys, the outside of pipeline two is provided with several reaction cavities, wax valve is respectively arranged between pipeline two and several reagent cavitys and several reaction cavities, homogeneous chemistry luminescence immunoassay technology is combined micro-fluidic carrier by the present invention, it is combined compared to out-phase chemiluminescence micro-fluidic, micro-fluidic design requirement is greatly reduced, simplify testing process, reduce error, introduce induced with laser wax threshold switch, so that micro-fluidic chip operation is more convenient efficient.
Description
Technical field
The present invention relates to electrochemiluminescent immunoassay detection technique field, especially a kind of micro-fluidic core to be shone based on homogeneous chemistry
Piece.
Background technique
Chemiluminescence immune assay (chemiluminescence immunoassay, CLIA), is will have high sensitivity
Chemical luminescent detecting technology combined with the immune response of high specific, for various antigens, haptens, antibody, hormone,
The detection and analysis technology of enzyme, fatty acid, vitamin and drug etc..By whether there is separation cleaning step, it is divided into out-phase chemistry hair
Light method and homogeneous chemistry luminescence method.Currently, diagnosing detection field in vitro, domestic and international testing product is substantially all using out-phase chemistry
Luminescence method.External producer includes Roche, Abbott Laboratories, Beckman, Siemens, the Meikang Suo Lin and Xi Si etc., and domestic manufacturer includes new produces
Industry, Antu, mikey, Mai Rui, pool are at, long brilliance doctor etc..Out-phase chemoluminescence method depends on physical separation, separates typical require
Be that crucial reactant is optionally immobilized on some solid substrates so that some physical processes for example filter, deposit, coalescing or
Person's magnetic separation can be used；And washing step is required, also to remove free ingredient.Therefore out-phase chemoluminescence method is whole
A analytic process step is more, time-consuming, complicated for operation, at high cost, and professional technician is needed to operate Special instrument in most cases
Device.And homogeneous chemistry luminescence immunoassay directly carries out chemiluminescence inspection without separation and cleaning step under pure liquid-phase condition
It surveys, it is easy to operate quick, it is suitble to POCT on-site test.
It is external at this stage to only have one homogeneous chemistry luminescence method (light-induced chemiluminescent) mediated using singlet oxygen of Siemens
Launch, detection need special LOCI module.LOCI technology is single step chemiluminescence sandwich immune detecting method, reagent
In containing there are two types of synthesis pearl reagent and a kind of biotin chemistry monoclonal antibody.The first pearl reagent (sensitive pearl) is coated with chain
Mould Avidin, and contain photaesthesia dyestuff；Second of pearl reagent (chemical pearl) is coated with another antibody, and contaminates containing chemiluminescence
Material；Sample and chemical pearl and biotinylated antibody form sandwich complex after being incubated for；Then sensitive pearl is added, with biotin
In conjunction with the rear immune complex for forming aggregation；Compound sensitive pearl therein under the irradiation of 680nm light can generate singlet oxygen, single
Line state oxygen can cause chemiluminescence reaction after permeating into chemical pearl, and generated chemiluminescence is reacted in measurement under 612nm wavelength
Signal.This method the high requirements on the equipment, marker material is special, is not easy to obtain.
Microfluidic chip technology (Microfluidics) is biological, chemical, medical analysis process sample preparation, instead
It the basic operation units such as answers, separate, detecting be integrated on the chip of one piece of micro-meter scale, being automatically performed analysis overall process.Due to
It in the great potential in the fields such as biology, chemistry, medicine, have been developed as a biology, chemistry, medicine, fluid, electronics,
The brand-new research field of the subject crossings such as material, machinery.In the application of current medical industry, it is concentrated mainly on detection field, it is micro-
Fluidics can combine the technologies such as immunochromatographiassays assays, fluoroimmunoassay, out-phase chemiluminescence immune assay as carrier,
There is corresponding product both at home and abroad.Since the application of microflow control technique is there is also certain barrier, the country is mating accordingly not enough
It is perfect, along with introducing other relative complex immuno analytical methods in microfluidic platform, product quality with foreign countries there is also compared with
Big gap, thus easy immuno analytical method is only the direction of development in conjunction with micro-fluidic carrier.
Detailed description of the invention
Fig. 1 is a kind of luminous microfluidic chip structure schematic diagram of homogeneous chemistry of the invention.
Fig. 2-1, Fig. 2-2 and Fig. 2-3 are that micro-fluidic chip of the invention carries out blood plasma (serum) separation and quantitative mistake to whole blood
The schematic diagram of journey.
Fig. 3-1, Fig. 3-2 and Fig. 3-3 are that micro-fluidic chip of the invention is diluted mixing and mixing to blood plasma (serum)
Liquid mean allocation process schematic.
Fig. 4-1, Fig. 4-2 and Fig. 4-3 are the process schematic that sample of the invention is sufficiently reacted with antibody one, antibody two.
Fig. 5-1, Fig. 5-2 and Fig. 5-3 are the reaction of reagent R3 of the invention and the quantitative adding procedure schematic diagram of R4.
Fig. 6 is signal acquisition testing process of the invention.
Fig. 7-1-1, Fig. 7-1-2, Fig. 7-2-1 and Fig. 7-2-1 are the on-off principle process of induced with laser wax valve of the invention
Schematic diagram, wherein Fig. 7-1-1 and Fig. 7-2-1 is the top view of wax valve of the invention, and Fig. 7-1-2 and Fig. 7-2-2 are of the invention
The side sectional view of wax valve.
In figure: 1 chip body, 2 injection ports, 3 air holes one, 4 sample cavitys, 5 quantitative cavitys, 6 pipelines one, 7 waste liquid chambers
Body, 8 mix cavity, 9 dilution cavitys, 10 pipelines two, 11-1 reaction cavity one, two, 13,14 reagent chamber of 12-1 reaction cavity
Body, wax valve V1, wax valve V2, wax valve V3, wax valve V4, wax valve V5, wax valve V6,15 air holes two, 16 rotation card slot, 17 pipelines, 18
Trapezoidal groove, 19 paraffin accommodating chambers.
Summary of the invention
In view of the above-mentioned problems existing in the prior art, that the present invention provides a kind of testing processes is simplified, error is low, operation more
The micro-fluidic chip efficiently to be shone based on homogeneous chemistry.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of micro-fluidic chip to be shone based on homogeneous chemistry, including chip body, the chip body is in the form of annular discs, core
Piece main body is provided centrally with rotation card slot, and the outside of the rotation card slot is annularly provided with sample cavity, quantitative cavity, gives up
Sap cavity body and dilution cavity, described quantify are provided with mixing cavity on the outside of cavity, waste liquid cavity and dilution cavity,
It is provided with injection port on the sample cavity, wax valve, the quantitative chamber are provided between sample cavity and quantitative cavity
It is connected between body and waste liquid cavity by pipeline one, is respectively set between the mixing cavity and quantitative cavity and dilution cavity
There is wax valve,
Pipeline two is provided on the outside of the mixing cavity, the outside of above-mentioned cavity is annularly arranged in pipeline two, mixes
Wax valve is provided between cavity and pipeline two, the inside of the pipeline two is additionally provided with several reagent cavitys, the outside of pipeline two
Several reaction cavities are provided with, are respectively arranged with wax valve between the pipeline two and several reagent cavitys and several reaction cavities.
Further, air hole one is additionally provided on the sample cavity.
Further, the rotation card slot is rounded, and one week spaced set of the rotation card slot has several keyways.
Further, the pipeline two is semi-enclosed pipeline, and one end of pipeline two is closed, the other end and reagent chamber
Body connection.
Further, several reaction cavities include several reaction cavities one and several reaction cavities two, described several
Reaction cavity one is set in qually spaced in the outside of pipeline two, and one is a pair of between several reaction cavities two and several reaction cavities one
It answers, is provided with wax valve between every group of reaction cavity one and reaction cavity two.
Further, air hole two is provided on the pipeline two.
Further, the wax valve includes pipeline, and arranged symmetrically on the pipeline there are two trapezoidal grooves, and two trapezoidal recessed
Pipeline between slot is connected with paraffin accommodating chamber, and there are two laser-irradiated domains for setting on the wax valve.
Further, the cross section of the pipeline is in rectangle, and the height of the pipeline is 100-400 μm.
Further, the depth of the trapezoidal groove is 500-1000 μm.
Further, the paraffin accommodating chamber is laser-irradiated domain one, the duct section between described two trapezoidal grooves
For laser-irradiated domain two.
It is using the beneficial effect of technical solution of the present invention:
(1) present invention is a kind of micro-fluidic chip based on disc type, is existing rank compared to chip, cassette, drop formula technology
The optimal selection of section multiple determination is at most able to achieve the detection of corresponding 12 indexs of a sample.
(2) chip is able to achieve the separation of whole blood sample, obtains serum or blood plasma.
(3) isolated serum or blood plasma can be realized dilution and it is rationed.
(4) homogeneous chemistry luminescence immunoassay technology is combined micro-fluidic carrier by the present invention, is sent out compared to out-phase chemistry
Light combine it is micro-fluidic, micro-fluidic design requirement is greatly reduced, simplified testing process, reduce error.
(4) present invention introduces induced with laser wax threshold switch, so that micro-fluidic chip operation is more convenient efficient.
Specific embodiment
Below by specific embodiment, invention is further described in detail.Unless stated otherwise, embodiment party below
Technology used in formula is routine techniques known to those skilled in the art.
" one embodiment " or " embodiment " referred to herein, which refers to, may be included at least one implementation of the invention
A particular feature, structure, or characteristic." in one embodiment " that different places occur in the present specification not refers both to same
A embodiment, nor the individual or selective embodiment mutually exclusive with other embodiments.
A kind of micro-fluidic chip to be shone based on homogeneous chemistry, including chip body 1, chip body 1 is in the form of annular discs, disk
Shape structure is the optimal selection of multiple determination, the center setting of chip body 1 compared to chip, cassette, drop formula technology
There is rotation card slot 16, after adding sample, chip is put into matched detecting instrument, and rotation card slot 16 is inserted into the appropriate position of necessary instrument
It sets, so that chip can rotate in detecting instrument, the outside of rotation card slot 16 is annularly provided with sample cavity 4, determines
Cavity 5, waste liquid cavity 7 and dilution cavity 9 are measured, the outside of quantitative cavity 5, waste liquid cavity 7 and dilution cavity 9 is provided with mixed
Even cavity 8, the sample cavity 4, quantitative cavity 5, waste liquid cavity 7, dilution cavity 9 in the present embodiment and the shape for mixing cavity 8
Shape size according to actual needs and degree voluntarily adjusted design handsome in appearance,
It is provided with injection port 2 on sample cavity 4, is provided with wax valve V1 between sample cavity 4 and quantitative cavity 5, quantitative cavity 5
It is connect between waste liquid cavity 7 by pipeline 1, mixes and be respectively set between cavity 8 and quantitative cavity 5 and dilution cavity 9
There are wax valve V2 and wax valve V3,
The outside for mixing cavity 8 is provided with pipeline 2 10, and the outside of above-mentioned cavity is annularly arranged in pipeline 2 10, above-mentioned
Cavity is i.e.: sample cavity 4, waste liquid cavity 7, mixes cavity 8 and dilution cavity 9 at quantitative cavity 5, mixes cavity 8 and pipeline two
Wax valve V4 is provided between 10, the inside of pipeline 2 10 is additionally provided with several reagent cavitys 13,14, and reagent cavity 13,14 is used for
Detection reagent is pre-installed, detection reagent can be liquid, be also possible to the pearl reagent of freeze-drying, the outside of pipeline 2 10 is provided with several
Reaction cavity is respectively arranged with wax valve V5 and wax valve V6, pipeline 2 10 between pipeline 2 10 and reagent cavity 13, reagent cavity 14
Wax valve V1-1 is respectively arranged between several reaction cavities.
Working principle:
Whole blood sample homogeneous chemistry shines testing process: being broadly divided into 3 parts, i.e. blood plasma (serum) separation of whole blood and fixed
Amount process；Blood plasma (serum) is diluted mixing and mixes liquid mean allocation.Antigen-antibody binding reaction, substrate catalysis are anti-
It answers.
The blood plasma (serum) of whole blood separates and dosing process
As shown in Fig. 2-1, a certain amount of whole blood enters sample cavity 4 by injection port 2, and designing in sample cavity 4 has
Stomata 1, air hole 1 are convenient for the addition of sample.After adding sample, chip is put into matched detecting instrument, rotates card slot 16
It is inserted into the appropriate location of necessary instrument.Chip starts to rotate, and under the influence of centrifugal force, erythroprecipitin is in sample cavity for whole blood
4 bottoms, blood plasma (serum) is then on top, as shown in Fig. 2-2.Chip stops operating, and opens V1 valve using laser at this time, such as schemes
Shown in 2-3, chip rotates again, and blood plasma (serum) enters quantitative cavity 5, and extra liquid enters waste liquid chamber by pipeline 1
Body 7.This process completes blood plasma (serum) quantitatively, improves the precision of detection.
Blood plasma (serum) is diluted mixing and mixes liquid mean allocation
As shown in figure 3-1, it is prefilled with dilution in dilution cavity 9, opens the V2 valve for closing quantitative cavity 5, and envelope
Close the V3 valve of dilution.Chip starts to rotate, and quantitative blood plasma (serum) and dilution enter mixing cavity 8, such as Fig. 3-2 simultaneously
It is shown.Chip swings, and after dilution and blood plasma (serum) are mixed well, chip stops rotating, laser opening V4 valve,
V1-1 valve, and the valve of the other function similar to V1-1, as shown in Fig. 3-3, the liquid of chip rotation, mixing passes through pipeline 2 10
Into one 11-1 of reaction cavity, and similar to other cavitys of one 11-1 of reaction cavity, design has air hole two on pipeline 2 10
15, which are more conducive to liquid, enters cavity.Due to being pre-loaded with R1 reagent in one 11-1 of reaction cavity, R1 reagent and liquid can be
Body is also possible to the pearl reagent of freeze-drying.So the liquid volume into one 11-1 of reaction cavity is the total of one 11-1 of reaction cavity
Volume subtracts the volume of R1 reagent.By taking immunochemiluminescence as an example, R1 reagent is primary antibody reagent, the antigen in blood plasma (serum) in
Primary antibody sufficiently reacts.
Antigen-antibody binding reaction, substrate catalysis reaction.
As shown in Fig. 4-1, the valve of corresponding V1-2 and similar functions is opened.Chip rotates again, and antigen-primary antibody is abundant
The liquid of reaction enters two 12-1 of reaction cavity and similar function cavity.Due to being pre-loaded with R2 in two 12-1 of reaction cavity
Reagent, R2 reagent can be liquid, or freeze-drying pearl reagent.Blood plasma (serum), the primary antibody of R1, R2 secondary antibody reagent together
It is reacted in two 12-1 of reaction cavity, chip swings, so that reaction is sufficiently, as shown in the Fig. 4-2.Sufficiently after reaction, chip
It stops rotating, 37 DEG C of incubation 5min.During this, while laser shutdown V1-2 valve and similar duty valve are utilized, opens V5
Valve, as shown in Fig. 4-3.Chip rotates again, and the R3 reagent being prefilled in reagent cavity 13 enters in one 11-1 of reaction cavity,
Dosing process is completed, as shown in fig. 5-1.Chip stops rotating, and opens V1-2 valve and similar duty valve, after opening valve, core
Piece rotates again, and R3 reagent enters two 12-1 of reaction cavity, as shown in Fig. 5-2.Chip swings, so that after reaction sufficiently,
Chip stops rotating.During this, while laser shutdown V1-2 valve and similar duty valve are utilized, opens V6 valve, chip is again
It rotates, the reagent R4 in reagent cavity 14 enters in one 11-1 of reaction cavity, as shown in Fig. 5-3.Chip stops rotating at this time, swashs
Light first opens before V1-2 valve and similar duty valve 4, as in Figure 6-1.Chip rotation, R4 reagent enter reaction cavity two
In 12-1, to play luminescence-producing reaction, signal detection is carried out to corresponding cavity.Divide 3 groups, shines respectively to the reaction of 12 cavitys
Signal is detected.
The principle of induced with laser wax threshold switch
Stain in chip is wax valve.Paraffin in wax valve has eutectic dot characteristics, wherein contains nano metal particles in wax
Son, all when laser irradiation paraffin, nano metal particles moment heat absorption, heat quickly passes to the paraffin of low melting point, wax
Valve is melted in 0.5 second, and can complete wax valve by closing to opening.Conversely, work as wax source, i.e., when a large amount of wax is irradiated with a laser,
Paraffin expansion, the paraffin of expansion enter chip pipeline, and the temperature of chip pipeline is relatively low, and instantaneous expansion enters chip pipeline
Paraffin, blocking pipe, wax valve complete the function by reaching pass.As shown in Fig. 7-1, the top view of paraffin valve and side solution
Cut open figure.When laser irradiation to two A2 of radiation area, the wax valve of closed state, after laser irradiation, paraffin (w) dissolution flows into chip
In two trapezoidal grooves in, pipeline 17 is opened, as shown in Fig. 7-2.When laser irradiation is to one A1 of radiation area, such as Fig. 7-2
Shown, paraffin expansion, the paraffin of expansion enters pipeline 17, as shown in Fig. 7-1.Wax valve completes the function by reaching pass.Paraffin
Switch is completed due to irradiation site difference, and operation can be repeated several times in the switch of paraffin valve.
As a preferred embodiment, air hole 1, air hole are additionally provided on the sample cavity 4 in the present embodiment
One 3 structure design facilitates the addition of sample.
As a preferred embodiment, the rotation card slot 16 in the present embodiment is rounded, and one week etc. of rotation card slot 16
Spacing is provided with several keyways, rotation card slot 16 is arranged to this structure, it can be ensured that during rotation, chip is fixed stably
It is fixed.
As a preferred embodiment, the pipeline 2 10 in the present embodiment is semi-enclosed pipeline, the one of pipeline 2 10
It is closed for holding, and the other end is connect with reagent cavity, and using this structure, the structure design of micro-fluidic chip is more reasonable, and shape is more
It is beautiful.
As a preferred embodiment, several reaction cavities in the present embodiment include several one 11-1 of reaction cavity and
Several two 12-1 of reaction cavity, several one 11-1 of reaction cavity are set in qually spaced in the outside of pipeline 2 10, several reaction cavities
It is corresponded between one 11-1 of two 12-1 and several reaction cavities, between every group of one 11-1 of reaction cavity and two 12-1 of reaction cavity
It is provided with wax valve V1-2, using this structure, the more compact structure of two 12-1 of one 11-1 of reaction cavity and reaction cavity thus may be used
More one 11-1 of reaction cavity and two 12-1 of reaction cavity to be arranged in chip body 1, so as to realize that multi objective is examined
It surveys, the detection of corresponding 12 indexs of a sample at most may be implemented.
As a preferred embodiment, air hole 2 15, pipeline 2 10 are provided on the pipeline 2 10 in the present embodiment
Upper setting air hole 2 15 is more conducive to liquid and enters in above-mentioned cavity.
As a preferred embodiment, the wax valve in the present embodiment includes pipeline 17, is symmetrically arranged with two on pipeline 17
A trapezoidal groove 18, the pipeline between two trapezoidal grooves 18 are connected with paraffin accommodating chamber 19, and there are two laser for setting on wax valve
Irradiation area, the switch of wax valve is adjusted by adjusting the irradiation position of laser, to realize opening and blocking up for pipeline, realizes reagent
With the circulation of sample.In a preferred embodiment, paraffin accommodating chamber 19 is one A1 of laser-irradiated domain, two trapezoidal grooves 18
Between 17 sections of pipeline be two A2 of laser-irradiated domain, using this structure, the position of laser-irradiated domain is fixed, so as to will
The transmitting position of laser is also fixed in equipment, can thus be accurately controlled the opening and closing of wax valve, so that micro-
Fluidic chip operation is more convenient efficient.
As a preferred embodiment, the cross section of the pipeline 17 in the present embodiment is in rectangle, and the height of pipeline 17 is
100-400 μm, the depth of trapezoidal groove 18 is 500-1000 μm, and the height of pipeline 17 and the setting of the depth of trapezoidal groove 18 are existed
Within the scope of this, the circulation of sample and reagent is more smooth, and the closing of pipeline 17 and opening also is more convenient, meanwhile, chip body 1 it is big
It is small also moderate.
Taking the above-mentioned ideal embodiment according to the present invention as inspiration, through the above description, relevant staff is complete
Various changes and amendments can be carried out without departing from the scope of the technological thought of the present invention' entirely.It is all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should all be included in the protection scope of the present invention.This
The technical scope of item invention is not limited to the contents of the specification, it is necessary to its technology is determined according to scope of the claims
Property range.
Claims (10)
1. a kind of micro-fluidic chip to be shone based on homogeneous chemistry, it is characterised in that: including chip body, the chip body is in
Discoid, chip body is provided centrally with rotation card slot, and the outside of the rotation card slot is annularly provided with sample cavity, determines
Cavity, waste liquid cavity and dilution cavity are measured, is provided with mixing on the outside of quantitative cavity, waste liquid cavity and the dilution cavity
Cavity,
Be provided with injection port on the sample cavity, be provided with wax valve between sample cavity and quantitative cavity, the quantitative cavity with
It is connected between waste liquid cavity by pipeline one, is respectively arranged with wax between the mixing cavity and quantitative cavity and dilution cavity
Valve,
Pipeline two is provided on the outside of the mixing cavity, the outside of above-mentioned cavity is annularly arranged in pipeline two, mixes cavity
Wax valve is provided between pipeline two, the inside of the pipeline two is additionally provided with several reagent cavitys, the outside setting of pipeline two
There are several reaction cavities, is respectively arranged with wax valve between the pipeline two and several reagent cavitys and several reaction cavities.
2. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: the sample
Air hole one is additionally provided on cavity.
3. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: the rotation
Card slot is rounded, and one week spaced set of the rotation card slot has several keyways.
4. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: the pipeline
Two be semi-enclosed pipeline, one end of pipeline two be it is closed, the other end is connect with reagent cavity.
5. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: described several
Reaction cavity includes several reaction cavities one and several reaction cavities two, and several reaction cavities one are set in qually spaced in pipeline
Two outside corresponds between several reaction cavities two and several reaction cavities one, every group of reaction cavity one and reaction cavity
Wax valve is provided between two.
6. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: the pipeline
Air hole two is provided on two.
7. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 1, it is characterised in that: the wax valve
It is symmetrical arranged the pipeline there are two trapezoidal groove, between two trapezoidal grooves including pipeline, on the pipeline and is connected with paraffin appearance
Receive chamber, there are two laser-irradiated domains for setting on the wax valve.
8. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 7, it is characterised in that: the pipeline
Cross section be in rectangle, the height of the pipeline is 100-400 μm.
9. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 7, it is characterised in that: described trapezoidal
The depth of groove is 500-1000 μm.
10. a kind of micro-fluidic chip to be shone based on homogeneous chemistry according to claim 7, it is characterised in that: the stone
Wax accommodating chamber is laser-irradiated domain one, and the duct section between described two trapezoidal grooves is laser-irradiated domain two.
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| CN110308296A (en) * | 2019-07-10 | 2019-10-08 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory |
| CN111495445A (en) * | 2020-02-28 | 2020-08-07 | 南北兄弟药业投资有限公司 | Test tray and test system |
| CN111879760A (en) * | 2020-06-22 | 2020-11-03 | 武汉新烽光电股份有限公司 | Micro-reagent consumption water ammonia nitrogen detection method based on micro-fluidic disc chip |
| CN111879761A (en) * | 2020-06-22 | 2020-11-03 | 武汉新烽光电股份有限公司 | Micro-reagent-consumption water quality residual chlorine detection method based on micro-fluidic disc chip |
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| CN115791763A (en) * | 2022-10-20 | 2023-03-14 | 胜泰生科(广州)医疗科技有限公司 | Homogeneous phase luminescence detection device and application |
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