CN109937953B - Method for culturing, domesticating and breeding Glyptosternum maculatum Regan by using circulating water - Google Patents

Method for culturing, domesticating and breeding Glyptosternum maculatum Regan by using circulating water Download PDF

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CN109937953B
CN109937953B CN201910313574.7A CN201910313574A CN109937953B CN 109937953 B CN109937953 B CN 109937953B CN 201910313574 A CN201910313574 A CN 201910313574A CN 109937953 B CN109937953 B CN 109937953B
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韩世成
卢彤岩
刘红柏
张颖
王常安
徐黎明
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Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
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Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
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Abstract

A method for culturing, domesticating and culturing Glyptosternum maculatum by using circulating water belongs to the technical field of aquaculture. The technical problem that batch death occurs when artificially cultured Glyptosternum maculatum Regans grows to about 3.3cm is solved, and the technical key points of the invention are as follows: a plurality of culture tanks are erected in the glass fiber reinforced plastic pool, a plurality of gaps are distributed on the side wall of each culture tank, smooth stones or coarse sand grains in a river channel are overlapped in the culture tanks, water is distributed into the culture tanks by water distribution pipes, a water return pipe is arranged at the bottom of the glass fiber reinforced plastic pool and is connected with a water level control pipe, and a water outlet pipe is connected to the outer wall of the water level control pipe; the mobile biological treatment tank is connected with the water outlet pipe, the mobile biological treatment tank, the connecting pipe, the fixed biological treatment tank, the overflow pipe, the sand filter tank, the purification tank and the aeration tank are sequentially communicated, the aeration tank is provided with a circulating pump, a temperature detection controller and a PH value monitoring detector, and the circulating pump, the ultraviolet sterilizer and the water inlet main pipe are sequentially connected. The invention is used for culturing and domesticating the Glyptosternum maculatum.

Description

Method for culturing, domesticating and breeding Glyptosternum maculatum Regan by using circulating water
Technical Field
The invention relates to a culture method for culturing and domesticating Glyptosternum maculatum Regans, in particular to a method for culturing and domesticating Glyptosternum maculatum Regans by circulating water, belonging to the technical field of aquaculture.
Background
Glyptosternum maculatum (Glyptosternum maculatum), Glyptosternum maculatum, Glyptosternum of Glyptosternaceae, named as Pagli in Tibetan, is mainly distributed in dry stream and branch stream of Glyptophan river from Yaluzang river Bobo river to Rika river in Tibet, is the only species distributed to middle and upstream in 7 Glyptophan family fishes in Yaluzang river, and the number of species tends to decrease in recent years.
Glyptosternum maculatum Regan has more quantity in middle swims of Yalu Tibetan Bujiang at the elevation of 4200m in Tibet, the yield is not large, but the meat quality is tender, the taste is delicious, the interspinal bones are few, the nutritive value is high, the Glyptosternum maculatum Regan is a rare cold water fish, the market value is high, the Glyptosternum maculatum Regan is deeply loved by people, besides the edible value, the Glyptosternum maculatum Regan can be used as a medicine, the Glyptosternum maculatum Regan has certain fishery economic value, and the Glyptosternum macula. However, due to slow growth speed, late sexual maturity, weak individual difference fertility, artificial over-fishing, invasion of foreign species and the like, wild Glyptosternum maculatum Regans is gradually exhausted, and is listed as an endangered species, and the germ plasm resource protection, artificial cultivation domestication and propagation scale of Glyptosternum maculatum Regans are carried out slowly.
In the prior art:
example 1, applicant is university of agriculture in china, application No. CN201310699056.6, entitled inventive patent application for a method for cultivating fry and fingerlings of Glyptosternum maculatum Regans using artificial feed, which discloses the following: the method of the invention utilizes clean river water source in an indoor cement pond, manually controls micro-flowing water, feeds artificial compound feed, and breeds the fish fry hatched from fertilized eggs into fish seeds with the full length of 3 cm. The survival rate of 2-3-day-old fries of Glyptosternum maculatum Regan cultured by the method reaches more than 80 percent when the fries are 3cm fingerlings.
In conclusion, the research content is how to artificially feed a method for cultivating a fry and a fingerling of Glyptosternum maculatum Regans, and the aim is to cultivate the fry of Glyptosternum maculatum Regans of 2-3 days old into the fingerling of 3cm in full length, and the survival rate reaches more than 80%.
Example 2, application No. CN201510837888.9, filed by academy of agriculture and animal husbandry in autonomous region of Tibet, invents an invention patent application named as a method for artificial breeding of Glyptosternum maculatum Regan, and discloses the following contents: the breeding method comprises the following steps: A. after the wild Glyptosternum maculatum Regans parent fish is soaked in salt water, performing male-female identification, and collecting female fish and male fish; b. The dosage of the pharmaceutical composition for female fish spawning induction is as follows: 5 mug/kg body weight of luteinizing hormone releasing hormone +1 carp pituitary/kg body weight; after 24h of injection, lightly pressing the abdomen of the female fish, and if no free egg granules flow out, injecting a second needle with the same dose of oxytocic; C. adopting dry fertilization; D. after the oosperm membrane of the fertilized egg falls off, the fertilized egg is poured into an incubation groove for incubation, when the Glyptosternum maculatum fries starts swimming at the bottom of the pond, exogenous nutrition is switched, and animal live baits and artificial mixed feed are fed. By mastering the mature rule of the wild Glyptosternum maculatum Regans parent fish and researching the artificial propagation technology of the Glyptosternum maculatum Regans, a set of feasible artificial induced spawning and artificial hatching method is formed, the artificial large-scale culture of the Glyptosternum maculatum Regans is realized, and the hatching rate reaches more than 70%.
In conclusion, in example 2, the research content is how Glyptosternum maculatum Regan is artificially bred, a set of feasible artificial induced spawning and artificial hatching methods is formed, artificial large-scale culture of Glyptosternum maculatum Regan is realized, and the hatching rate is more than 70%.
Example 3, the applicant is the research institute of aquatic science of academy of agriculture and animal husbandry in autonomous region of ***, application number CN201721438000.5, invents a utility model patent named Glyptophan maculatum fertilized egg hatching and young fish culture device, which discloses the following: during the hatching process of fertilized eggs of Glyptosternum maculatum Regan, the following problems are found to exist: the tail of the larval fish goes out of the membrane, but the head and the yolk sac still can not be separated from the egg membrane, and finally, the larval fish die in large batch because of the overlong membrane-going time. The technical task of artificial breeding of Glyptosternum maculatum is overcome. The technical scheme is as follows: the fertilized egg hatching and young fish culturing device of Glyptosternum maculatum Regans comprises a culturing tank, a water inlet pipe and a water outlet; the upper end of the culture tank is provided with an opening, an isolation screen is arranged in the culture tank, and the isolation screen divides the culture tank into a left part and a right part, one part is a culture chamber, and the other part is an oxygen supply chamber; an oxygen pump and a water inlet pipe are arranged in the oxygen supply chamber, and an incubation sieve tray is laid above the culture chamber; the bottom of the culture room is provided with the water outlet. The whole set of device for hatching the fertilized eggs of Glyptosternum maculatum and culturing the larvae fish can be used for the hatching process of the fertilized eggs, and the hatching mesh screen is taken away after the larvae fish are taken out of the membrane, so that the larvae fish is also a fry culturing device, and the device has high utilization rate and good practicability. The water outlet and the water inlet pipe form a water flow passage together, and the flowing water environment of the whole culture tank in the process of hatching the fertilized eggs of Glyptosternum maculatum and culturing the larvae is ensured. The isolating screen separates the culture chamber from the oxygen supply chamber, can provide more suitable environmental conditions for the incubation of fertilized eggs of Glyptosternum maculatum and the larvae, and can prevent bubbles from entering the culture chamber, thereby preventing the bubbles from impacting the larvae and preventing the fingerlings from swallowing small bubbles. The isolation screen separates the culture room from the oxygen supply room, and sponge is filled between the isolation screen and the culture groove wall, so that bubbles generated by the oxygen pump can be effectively prevented from rising and focusing to the bottom of the hatching sieve tray, and the situation that fertilized eggs are anoxic and dead due to bubble isolation and incapability of contacting with water flow is avoided. When passing through the sieve tray, the size limitation of the sieve tray grids can play an effective role in helping most of embryo bodies to completely remove egg membranes, and the membrane removal rate reaches over 95 percent.
To sum up, in the above example 3, the cultivated object is wild Glyptosternum maculatum larvae juvenile fish, and the effect is realized by helping most of embryo bodies to completely break away from egg membranes, and the membrane removal rate reaches more than 95%.
Example 4, the applicant is the research institute of aquatic science of academy of agriculture and animal husbandry in autonomous region of Tibet with application number CN201820610981.5, inventing a utility model patent entitled culture system for domesticating wild Glyptosternum maculatum kisans parent fish, which discloses the following: in order to realize the domestication of wild Glyptosternum maculatum Regans, a culture system for domesticating the parent Glyptosternum maculatum Regans is provided, the ecological environment is simulated, and the domestication and seed preservation survival rate of the parent Glyptosternum maculatum Regans is effectively improved. The technical scheme is as follows: the culture system comprises a filter tank, an oxygenation tank, a temperature control device and a culture tank; the water outlet of the filter tank is connected with the water inlet of the aeration tank, the water outlet of the aeration tank is connected with the water inlet of the temperature control device, the water outlet of the temperature control device is connected with the water inlet of the culture tank, and the water outlet of the culture tank is connected with the water inlet of the filter tank; an arch channel for parent fish to pass through is laid at the bottom of the culture pond. According to the system, the arched channel is laid at the bottom of the culture pond, a cave in a natural ecological environment is simulated, accurate temperature control is realized by the temperature control device, the occurrence of the saprolegnia Glyptosternum Regans can be effectively controlled, water is saved, the domestication and seed conservation survival rate of the parent Glyptosternum Regans can be effectively improved, and the survival rate can be improved to more than 90%.
To sum up, in example 4, the cultivated object is wild Glyptosternum maculatum Regans parent fish, and the survival rate can be improved to more than 90%.
Under the efforts of scientific researchers, in the hatching process of fertilized eggs of Glyptosternum maculatum Regans, the aim of helping most of embryo bodies to completely shed egg membranes is realized, and the membrane shedding rate reaches more than 95%; the parent Glyptosternum maculatum Regans fish domesticates and preserves the survival rate, the survival rate is improved to more than 90%; the survival rate of cultivated 2-3 days old fry of Glyptosternum maculatum Regan cultivated into 3 cm-length fingerlings reaches more than 80%. The juvenile Glyptosternum maculatum Regans feeds after the meal is opened, but batch death occurs when the juvenile Glyptosternum maculatum Regans grows to about 3.3 centimeters. In a sense, the current scientific researchers face a difficulty that the central importance of the work of the scientific researchers is to break through the bottleneck and artificially culture the 1 st and 2 nd Glyptophan maculatum Regans.
Disclosure of Invention
The following presents a simplified summary of the invention in order to provide a basic understanding of some aspects of the invention. It should be understood that this summary is not an exhaustive overview of the invention. It is not intended to determine the key or critical elements of the present invention, nor is it intended to limit the scope of the present invention. Its sole purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is discussed later.
In view of the above, in order to overcome the technical problem that batch death occurs when the artificially cultured Glyptosternum maculatum Regans grows to about 3.3 centimeters, the invention provides a method for culturing, domesticating and culturing the Glyptosternum maculatum Regans by using circulating water, so that 1-age and 2-age Glyptosternum maculatum Regans are obtained through artificial culturing and circulating water culturing, the reasonable utilization and protection of Glyptosternum maculatum Regans resources are further promoted, and the sustainable development of the cold water fish industry in Tibet is promoted.
The invention relates to a method for culturing, domesticating and culturing Glyptosternum maculatum Regan by using circulating water, wherein a circulating water culture water body enters an ultraviolet sterilizer through a pipeline by a circulating pump, and after the water body is sterilized, the circulating water culture water body enters a water inlet main pipe, flows through water inlet branch pipes and respectively enters a culture tank through a water distribution pipe; smooth stones or coarse sand of a river channel are filled in the culture tank, and Glyptosternum maculatum is cultured under artificially simulated natural environment conditions; the water flow state in the culture tank is changed by adjusting the direction of the water distribution pipe; the water in the culture tank flows into the circulating culture tank through pores on four walls, enters the water level control pipe through the water return pipe, flows in and out the water pipe, enters the mobile biological treatment tank through the water outlet pipe, enters the fixed biological treatment tank through the connecting pipe, flows into the sand filter through the overflow pipe after treatment, then enters the purification tank, finally enters the aeration tank, and is aerated by the aerator to adjust the dissolved oxygen in the water; automatically detecting p H by using a water p H value monitoring detector, and controlling the value of the aquaculture water p H; the temperature of the culture water body is automatically detected by using a temperature detection controller, the set water temperature of the culture is controlled, and the whole culture circulating water treatment process is completed; the adjusted aquaculture water is pumped away by the circulating pump, so that the aquaculture water enters the next circulating water treatment process.
Further: circulating water for aquaculture enters the ultraviolet sterilizer through a pipeline by a circulating pump, and after the water is sterilized, the water enters a water inlet main pipe, flows through a water inlet branch pipe and passes through a valve, and then enters the culture tank through water distribution holes; smooth stones or coarse sand of a river channel are filled in the culture tank, and Glyptosternum maculatum is cultured under artificially simulated natural environment conditions; the water flow state in the culture tank is changed by adjusting the direction of the water distribution holes; the water in the culture tank flows into the circulating culture tank through pores on the four walls, enters the water level control pipe through the return pipe, flows in and out the water pipe, enters the mobile biological treatment tank through the water outlet pipe, treats organic matters such as ammonia nitrogen, nitrate and nitrite in the water through the mobile biological filter material, generates gas by using the oxygenation pump, performs oxygenation and aeration on the water in the mobile biological treatment tank through the oxygenation aeration pipe, and ensures the gas flow direction by using the gas distribution plate, so that the biological filter material moves up and down uniformly and regularly; water in the mobile biological treatment tank passes through a first filter screen and a connecting pipe, enters a fixed biological treatment tank through a first filter screen, passes through a fixed biological filter material, retreats organic matters such as ammonia nitrogen, nitrate, nitrite and the like in the water, flows into a sand filter tank through a second filter screen and an overflow pipe after treatment, uniformly distributes the water above the sand filter tank through a first water distribution plate, filters fine particle suspended matters in the water by using filter sand, enters a purification tank after passing through a second filter screen, distributes the water by a second water distribution plate, purifies and adsorbs micro impurities in the water by using activated carbon, enters an oxygenation tank after p H hardness of the water quality of the water is adjusted by corallite, and is oxygenated by using an oxygenator to adjust dissolved oxygen in the water; automatically detecting p H by using a water p H value monitoring detector, and controlling the value of the aquaculture water p H; the temperature of the culture water body is automatically detected by using a temperature detection controller, the set water temperature of the culture is controlled, and the whole culture circulating water treatment process is completed; the adjusted aquaculture water is pumped away by the circulating pump, so that the aquaculture water enters the next circulating water treatment process.
The cultivation system realized by the method comprises a circulating pump, an ultraviolet sterilizer, a water inlet main pipe, a temperature detection controller, a water distribution pipe, a cultivation tank, a glass fiber reinforced plastic pond, a water level control pipe, a water outlet pipe, a mobile biological treatment pond, a water return pipe, a connecting pipe, a fixed biological treatment pond, an overflow pipe, a sand filter pond, a purification pond, an oxygenation pond, an p H value monitoring detector and a circulation treatment device supporting structure;
a plurality of culture grooves are erected in the glass fiber reinforced plastic pond, a plurality of gaps are distributed on the side wall of each culture groove, and smooth stones or coarse sand grains in a river channel are overlapped in the culture grooves; the water inlet main pipe is arranged above the culture tank, a plurality of water inlet branch pipes are arranged on the water inlet main pipe, the water outlet ends of the water inlet branch pipes are connected with water distribution pipes, the water distribution pipes are vertically arranged in the culture tank, the bottom of the glass fiber reinforced plastic pool is provided with a water return pipe, the water return pipe is connected with a water level control pipe, and the outer wall of the water level control pipe is connected with a water outlet pipe; a circulating treatment device supporting body is arranged below the glass fiber reinforced plastic tank, and the fixed biological treatment tank, the sand filter tank, the purification tank and the oxygenation tank are sequentially arranged in a circulating treatment device supporting structure from right to left; the right side below that glass steel pond was arranged in to the removal biological treatment pond, remove biological treatment pond and go out water piping connection, the lower part that removes the biological treatment pond is through connecting tube and fixed biological treatment pond UNICOM, the overflow pipe is installed on the upper portion in fixed biological treatment pond, the outflow water of overflow pipe gets into husky filtering pond, the bottom of husky filtering pond and the bottom UNICOM of purifying tank, the water body that the purifying tank flows enters the oxygenation pond, be provided with the circulating pump on the oxygenation pond, temperature detect controller and p H value monitoring detector, the circulating pump, ultraviolet disinfector and water inlet main pipe connect gradually, the circulating pump is used for pumping the water in the oxygenation pond into ultraviolet disinfector, the oxygenation ware is used for oxygenation in to the oxygenation pond.
Further: the lateral wall cloth of breeding the groove has a plurality of gaps, specifically does: a plurality of rectangular holes are formed in the side wall in an array mode, the length and the width of each rectangular hole are 30mm and 1.5mm, the distance between the vertically adjacent rectangular holes is 2mm, and the distance between the horizontally adjacent rectangular holes is 4 mm. By the arrangement, under the condition of preventing juvenile fishes from escaping, the water distribution pipes distribute water into the culture tank, and the natural growth environment of Glyptosternum maculatum is simulated more truly.
Further: the water distribution pipe is provided with a plurality of water distribution holes from top to bottom, the water distribution holes are arranged on the same bus of the water distribution pipe, and the water outlet end of the water inlet branch pipe is connected with the water distribution pipe through a loose joint. So set up, the adjustable connection form of trompil mode combination water distributor changes the rivers state in breeding the groove through adjustment water distribution hole direction, comes the simulation natural environment rivers condition.
Further: and the water inlet end of each water distribution pipe is provided with a respective valve. According to the arrangement, the water distribution pipes can be coordinately managed, the water flow speed is adjusted by adjusting the opening degrees of different valves, and the natural growth environment of Glyptosternum maculatum Regan is simulated.
Further: the length, width and height of each culture groove are 1500mm 1200mm 1100mm, the height of culture water level is 900mm, and the height of coarse sand grains in smooth stones or river channels is 500 mm. By the arrangement, the Glyptosternum maculatum Regans is suitable for culturing and domesticating Glyptosternum maculatum Regans with the body length of 3.3 centimeters to 1-year and 2-year Glyptosternum maculatum Regans.
Further: the mobile biological treatment tank comprises a first tank body, a first filter screen, a mobile biological filter material, an oxygenation pump and an oxygenation aeration pipe; set up first filter screen in the first cell body, have the removal biological filter material in the first filter screen, first cell body bottom is arranged in to oxygenation aeration pipe level, and oxygenation aeration pipe is through arranging outside oxygenation pump in to the inside oxygenation of first cell body.
Further: the mobile biological treatment tank also comprises an air distribution plate, and the middle part of the oxygen aeration pipe is vertically provided with the air distribution plate. So set up, prevent that gas from concentrating on a certain position, the gaseous dispersion is gaseous better, utilizes the gas distribution board to guarantee the gas flow direction to make biological filter material even, regularly reciprocate.
Further: the fixed biological treatment tank comprises a second tank body, a first filter plate, a fixed biological filter material and a second filter screen, wherein the second tank body is internally provided with the first filter plate, the fixed biological filter material and the second filter screen from bottom to top.
Further: the sand filter comprises a third tank body, a first water distribution plate, sand filter and a second filter plate, wherein the third tank body is internally provided with the first water distribution plate, the sand filter and the second filter plate from top to bottom.
Further: the purifying tank comprises a fourth tank body, a second water distribution plate, activated carbon and corallite, and the second water distribution plate, the activated carbon and the corallite are arranged in the fourth tank body from bottom to top.
Has the advantages that:
the invention aims to break through the technical bottleneck that batch death occurs when artificially cultured Glyptosternum maculatum Regans grows to about 3.3cm, through assiduous research for three years, the artificially cultured Glyptosternum maculatum Regans culture and domestication method disclosed by the invention obtains and domesticates 1-year and 2-year Glyptosternum maculatum Regans by adopting the artificial culture and the cyclic water culture, the average body length of the 1-year Glyptosternum maculatum Regans reaches 6.55cm, the average body weight reaches 16.13g, the average body length of the 2-year Glyptosternum maculatum Regans reaches 9.31cm, the average body weight reaches 22.86g, the survival rate of the 1-year Glyptosternum maculatum Regans reaches 60.52%, and the survival rate of the 2-year Glyptosternum maculatum Regans reaches 57.86%. The survival rates of 1 st and 2 nd Glyptosternum maculatum Regan are rarely reported in the existing data, because the survival rates of 1 st and 2 nd Glyptosternum maculatum Regan in a large number of research and culture processes are extremely low, the research of the invention achieves unexpected results of research and development personnel in the field, and the reasonable utilization and protection of Glyptosternum maculatum Regan resources are promoted, and the sustainable development of the Tibetan cold water fish industry is promoted.
Drawings
FIG. 1 is a front view of a method for culturing and domesticating Glyptosternum maculatum Regn by using circulating water according to the present invention;
FIG. 2 is a top view of the distribution of the culture tanks in FIG. 1 (several culture tanks are omitted from the middle of two culture tanks);
FIG. 3 is a partially enlarged view of the culture tank;
fig. 4 is a front view of embodiment 2 of the present invention.
In the figure: circulating pump 1, ultraviolet sterilizer 2, water inlet main pipe 3, temperature detection controller 4, water distributor 5, culture tank 6, glass steel pond 7, water level control pipe 8, outlet pipe 9, mobile biological treatment pond 10, wet return 11, connecting pipe 12, fixed biological treatment pond 13, overflow pipe 14, sand filter 15, purification pond 16, oxygenation pond 17, p H value monitoring detector 18, circulation processing apparatus bearing structure 19, water inlet branch pipe 20, smooth stone 21, coarse sand grain 22 in the river course.
Detailed Description
Exemplary embodiments of the present invention will be described hereinafter with reference to the accompanying drawings. In the interest of clarity and conciseness, not all features of an actual implementation are described in the specification. It will of course be appreciated that in the development of any such actual embodiment, numerous implementation-specific decisions must be made to achieve the developers' specific goals, such as compliance with system-related and business-related constraints, which will vary from one implementation to another. Moreover, it will be appreciated that such a development effort might be complex and time-consuming, but would nevertheless be a routine undertaking for those of ordinary skill in the art having the benefit of this disclosure.
It should be noted that, in order to avoid obscuring the present invention with unnecessary details, only the device structures and/or processing steps closely related to the solution according to the present invention are shown in the drawings, and other details not so relevant to the present invention are omitted.
The specific implementation mode is as follows: as shown in fig. 1-3, the present embodiment provides a method for culturing and domesticating Glyptosternum maculatum Regans by using circulating water, which comprises a circulating pump 1, an ultraviolet sterilizer 2, a water inlet header pipe 3, a temperature detection controller 4, a water distribution pipe 5, a culture tank 6, a glass fiber reinforced plastic pond 7, a water level control pipe 8, a water outlet pipe 9, a mobile biological treatment pond 10, a water return pipe 11, a connecting pipe 12, a fixed biological treatment pond 13, an overflow pipe 14, a sand filter 15, a purification pond 16, an oxygenation pond 17, an p H value monitoring detector 18 and a circulating treatment device supporting structure 19;
the glass fiber reinforced plastic pond 7 is internally provided with a plurality of culture tanks 6, and the side wall of each culture tank 6 is provided with a plurality of gaps, which are specifically as follows: a plurality of rectangular holes are formed in the side wall in an array mode, the length and the width of each rectangular hole are 30mm and 1.5mm, the distance between the vertically adjacent rectangular holes is 2mm, and the distance between the horizontally adjacent rectangular holes is 4 mm; smooth stones or coarse sand grains in the river channel are overlapped in the culture grooves 6, the length, width and height of each culture groove are 1500mm, 1200mm and 1100mm, the height of the culture water level is set to be 900mm, and the overlapping height of the coarse sand grains in the smooth stones or the river channel is 500 mm; the water inlet header pipe 3 is arranged above the culture tank 6, a plurality of water inlet branch pipes 20 are arranged on the water inlet header pipe, the water outlet ends of the water inlet branch pipes 20 are connected with water distribution pipes 5, the water distribution pipes are provided with a plurality of water distribution holes 5-1 from top to bottom, the water distribution holes are arranged on the same bus of the water distribution pipes, the water outlet ends of the water inlet branch pipes are connected with the water distribution pipes through loose joints, and the water inlet end of each water distribution pipe is provided with a respective valve 5-2; the water distribution pipe 5 is vertically arranged in the culture tank 6, the bottom of the glass fiber reinforced plastic pond 7 is provided with a water return pipe 11, the water return pipe 11 is connected with a water level control pipe 8, and the outer wall of the water level control pipe 8 is connected with a water outlet pipe 9; a circulating treatment device supporting body 19 is arranged below the glass fiber reinforced plastic tank 7, and the fixed biological treatment tank 13, the sand filter 15, the purification tank 16 and the oxygenation tank 17 are sequentially arranged in the circulating treatment device supporting structure 19 from right to left; the movable biological treatment tank 10 is arranged at the lower right side of the glass fiber reinforced plastic tank 7, the movable biological treatment tank 10 is connected with a water outlet pipe 9, the lower part of the movable biological treatment tank 10 is communicated with a fixed biological treatment tank 13 through a connecting pipe 12, an overflow pipe 14 is arranged at the upper part of the fixed biological treatment tank 13, the effluent water body of the overflow pipe 14 enters a sand filter 15, the bottom of the sand filter 15 is communicated with the bottom of a purification tank 16, the effluent water body of the purification tank 16 enters an oxygenation tank 17, a circulating pump 1, a temperature detection controller 4 and an p H value monitoring detector 18 are arranged on the oxygenation tank 17, the circulating pump 1, an ultraviolet sterilizer 2 and a water inlet main pipe 3 are sequentially connected, and the circulating pump 1 is used for pumping the water body in the oxygenation tank; the oxygen increasing device is arranged outside and is used for increasing oxygen in the oxygen increasing tank;
the mobile biological treatment tank 10 comprises a first tank body 10-1, a first filter screen 10-2, a mobile biological filter material 10-3, an oxygenation pump 10-4, an oxygenation aeration pipe 10-5 and an air distribution plate 10-6; a first filter screen 10-2 is arranged in the first tank body 10-1, a mobile biological filter material 10-3 is arranged in the first filter screen 10-2, an oxygen aeration pipe 10-5 is horizontally arranged at the bottom of the first tank body 10-1, the oxygen aeration pipe 10-5 aerates oxygen into the first tank body 10-1 through an oxygen aeration pump 10-4 arranged outside, and an air distribution plate 10-6 is vertically arranged in the middle of the oxygen aeration pipe 10-5; the fixed biological treatment tank 13 comprises a second tank body 13-1, a first filter plate 13-2, a fixed biological filter material 13-3 and a second filter screen 13-4, wherein the first filter plate 13-2, the fixed biological filter material 13-3 and the second filter screen 13-4 are arranged in the second tank body 13-1 from bottom to top; the sand filter 15 comprises a third tank body 15-4, a first water distribution plate 15-1, filter sand 15-2 and a second filter plate 15-3, wherein the first water distribution plate 15-1, the filter sand 15-2 and the second filter plate 15-3 are arranged in the third tank body 15-4 from top to bottom; the purification tank 16 comprises a fourth tank body 16-1, a second water distribution plate 16-2, activated carbon 16-3 and corallite 16-4, wherein the second water distribution plate 16-2, the activated carbon 16-3 and the corallite 16-4 are arranged in the fourth tank body 16-1 from bottom to top.
The specific implementation mode is that the circulating water is utilized to carry out cultivation and domestication, water flow under natural conditions is simulated, the direction, the size and the impact position of the water flow in the flowing water cultivation tank are designed, and the formed water flow environment is like the natural condition state by proper adjustment according to the cultivation requirements. Circulating water for aquaculture enters the ultraviolet sterilizer 2 through a pipeline by the circulating pump 1, and after water is sterilized, enters the water inlet main pipe 3, flows through the water inlet branch pipe 20 and passes through the valve 5-2, and the water enters the culture tank 6 through the water distribution holes 5-1 respectively; the culture tank 6 is made of stainless steel materials, smooth stones 21 or coarse sand 22 of a river channel are filled in the culture tank 6, and Glyptosternum maculatum Regn is cultured under artificial simulated natural environment conditions; the water flow state in the culture tank 6 is changed by adjusting the direction of the water distribution holes 5-1; the water in the culture tank 6 flows into the circulating culture tank through the pores on the four walls, enters the water level control pipe 8 through the return pipe 11, flows into the water inlet pipe 9 and the water outlet pipe 9, enters the mobile biological treatment tank 10 through the water outlet pipe 9, treats organic matters such as ammonia nitrogen, nitrate, nitrite and the like in the water through the mobile biological filter material 10-3, generates gas by using an oxygenation pump 10-4, performs oxygenation and aeration on the water in the mobile biological treatment tank 10 through an oxygenation aeration pipe 10-5, and ensures the gas flow direction by using a gas distribution plate 10-6, so that the biological filter material moves up and down uniformly and regularly; the water in the mobile biological treatment tank 10 enters a fixed biological treatment tank 13 through a first filter screen 10-2 and a connecting pipe 12 and passes through a first filter plate 13-2, organic matters such as ammonia nitrogen, nitrate, nitrite and the like in the water are retreated through a fixed biological filter material 13-3, the treated water flows into a sand filter 15 through a second filter screen 13-4 and an overflow pipe 14, the water is uniformly distributed above the sand filter 15 through a first water distribution plate 15-1, fine particle suspended matters in the water are filtered by using the filter sand 15-2, the water enters a purification tank 16 after passing through a second filter plate 15-3, the water is distributed through a second water distribution plate 16-2, micro impurities in the water are purified and adsorbed by activated carbon 16-3, p H hardness of water quality of the water is adjusted by corallite 16-4, and then the water enters an oxygenation tank 17, utilizing an oxygenator to oxygenate the treated water body, and adjusting dissolved oxygen in the water body; automatically detecting p H by using a water p H value monitoring detector 18 and controlling the value of the aquaculture water p H; the temperature of the culture water body is automatically detected by using the temperature detection controller 4, the set water temperature of the culture is controlled, and the whole culture circulating water treatment process is completed; the adjusted aquaculture water is pumped away by the circulating pump 1, so that the aquaculture water enters the next circulating water treatment process.
Therefore, the culture and domestication of Glyptosternum maculatum Regan in circulating water by artificially simulating natural environmental conditions are realized. Obtaining artificially cultured and cultured by circulating water culture to domesticate 1-year and 2-year Glyptosternum maculatum Regans, wherein the average body length of the 1-year Glyptosternum maculatum Regans reaches 6.55cm, the average body weight reaches 16.13g, the average body height is 1.09cm, the average head length is 1.46cm, and the average tail stem length is 1.31 cm; the average body length of 2-year Glyptosternum maculatum Regan reaches 9.31cm, the average body weight reaches 22.83g, the average body height is 1.60cm, the average head length is 2.18cm, and the average tail stem length is 1.72 cm; the survival rate of 1-year Glyptosternum maculatum Regan reaches 60.52%, the constitution is strong, the body surface is smooth, the activity is strong, the ingestion is vigorous, the survival rate of 2-year Glyptosternum maculatum Regan reaches 57.86%, the constitution is strong, the body surface is smooth, the activity is strong, and the ingestion is vigorous.
Example 1: the difference between the present embodiment and the specific embodiment is that the ultraviolet sterilizer 2 and the temperature detection controller are removed, the water distribution pipe is installed outside the culture tank, the size of the culture tank is adjusted, the length, width and height are 1600mm, 1200mm, the height of the culture water level is set to 1000mm, a plurality of rectangular holes are arranged on the side wall in an array mode, the length, width and height of the rectangular holes are 40mm, 1.5mm, the juvenile fish are placed in the system for culture, the juvenile fish start to die when the juvenile fish grows to 2.73cm, and all the juvenile fish die after 6 days.
Example 2: referring to fig. 4, the difference between this embodiment and the specific embodiment lies in that smooth stones (stones, cobblestones, etc. washed in the river), coarse sand grains in the river, and the PE tube fish reef with holes are placed in the culture tank, and the three substances are placed in an overlapping manner under artificial simulation natural conditions, so as to generate three different fish culture living environments. The water flow under the natural condition is simulated, the water flow direction in the flowing water culture tank is designed, and the water flow environment formed by properly adjusting according to the culture requirement is like the natural condition state. Grouping temperature control: the Glyptosternum maculatum Regan is cultured and domesticated in running water, and the grouping temperature control is carried out by 5 temperature gradients in three different culture environments under the same water source, the same oxygenation mode and the conditions of water quality filtration and disinfection. The test is carried out in 5 groups of test culture tanks, and the temperature gradient is set to be 6-7 ℃; 8-9 ℃; 10-12 ℃; 13-15 ℃; 16-18 ℃. Feeding the feed: (nutrient supply) simulated natural flowing water culture conditions, an optimized water quality treatment method and technology, flowing water culture is carried out, full-artificial cultivation and domestication are carried out, cultivation and domestication of Glyptosternum maculatum Regans are carried out in the form of feeding live baits, compound feeds and bulk materials formed by mixing the live baits with the feeds, and feeding times and feeding amount are designed according to water temperature conditions. By adopting the method, the Glyptosternum maculatum Regans is cultured in running water by using circulating water, and a good effect is achieved in circulating water culture within a three-month culture period. The full-artificial cultivation and domestication are implemented by adopting the technologies of grouping temperature control, artificial design, supply of different feed nutrients and the like under different ecological conditions of cultivation environments and under the same oxygenation mode and water quality treatment conditions. In three culture environments with different temperature gradients, the temperature is 8-9 ℃, the survival rate in the environment of overlapping smooth stones and coarse sand grains is high, the survival rate of 1 st Glyptosternum maculatum Regan reaches 60.52%, the survival rate of 2 nd Glyptosternum maculatum Regan reaches 57.86%, and seedlings are strong in physique, smooth in body surface, strong in activity and vigorous in food intake. The temperature is 6-7 ℃, and the survival rate in three environments is lower than 19.5 percent. The temperature is 10-12 ℃, the survival rate in three environments is lower than 16.6 percent, the temperature is 13-15 ℃, and the survival rate in three environments is lower than 18.4 percent. The temperature is 16-18 ℃, and the survival rate in three environments is lower than 16.1%.
Although the embodiments of the present invention have been described above, the contents thereof are merely embodiments adopted to facilitate understanding of the technical aspects of the present invention, and are not intended to limit the present invention. It will be apparent to persons skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (2)

1. The method for culturing, domesticating and culturing the Glyptosternum maculatum by using circulating water is characterized by comprising the following steps of: the method comprises the steps of utilizing circulating water to carry out culture domestication, simulating water flow under natural conditions, designing the direction, the size and the impact position of the water flow in a flowing water culture tank, carrying out appropriate adjustment according to culture requirements to form a water flow environment such as a natural condition state, enabling a circulating water culture water body to enter an ultraviolet sterilizer (2) through a pipeline by a circulating pump (1), carrying out water body disinfection and sterilization, enabling the circulating water culture water body to enter a water inlet main pipe (3), enabling the circulating water body to flow through a water inlet branch pipe (20), and enabling the circulating water body to enter a culture tank (6) through a; smooth stones or coarse sand of a river channel are filled in the culture tank (6), and the Glyptosternum maculatum is cultured under artificially simulated natural environment conditions; the water flow state in the culture tank (6) is changed by adjusting the direction of the water distribution pipe; the water in the culture tank (6) flows into the circulating culture tank through pores on the four walls, enters the water level control pipe (8) through the water return pipe (11), flows into and out of the water pipe (9), enters the mobile biological treatment tank (10) through the water outlet pipe (9), enters the fixed biological treatment tank (13) through the connecting pipe (12), flows into the sand filter (15) through the overflow pipe (14), then enters the purification tank (16), finally enters the oxygenation tank (17), and is oxygenated by the oxygenator to adjust dissolved oxygen in the water; automatically detecting p H by using a water p H value monitoring detector (18) and controlling the value of the aquaculture water p H; the temperature of the aquaculture water body is automatically detected by using a temperature detection controller (4), and the set water temperature of the aquaculture is controlled to complete the whole aquaculture circulating water treatment process; the adjusted aquaculture water body is pumped away by the circulating pump (1), so that the aquaculture water body enters the next circulating water treatment process; culturing and domesticating Glyptosternum maculatum Regan with the body length of 3.3 centimeters to 1-year and 2-year Glyptosternum maculatum Regan;
the height of the culture water level is set to 900mm, the height of the overlapped placement of the coarse sand grains in the smooth stones or the river channels is 500mm, and the culture temperature is 8-9 ℃.
2. The method for culturing and domesticating culture of Glyptosternum maculatum Regn by using circulating water as claimed in claim 1, which is characterized in that: circulating water for aquaculture enters the ultraviolet sterilizer (2) through a pipeline by the circulating pump (1), and after water is sterilized, enters the water inlet main pipe (3), flows through the water inlet branch pipe (20) and passes through the valve (5-2), and the water enters the culture tank (6) through the water distribution holes (5-1); smooth stones (21) or coarse sand (22) of a river channel are filled in the culture tank (6), and Glyptosternum maculatum Regan is cultured under artificially simulated natural environment conditions; the water flow state in the culture tank (6) is changed by adjusting the direction of the water distribution holes (5-1); the water in the culture tank (6) flows into the circulating culture tank through pores on the four walls, enters the water level control pipe (8) through the water return pipe (11), flows into and out of the water pipe (9), enters the mobile biological treatment tank (10) through the water outlet pipe (9), treats ammonia nitrogen, nitrate and nitrite in the water through the mobile biological filter material (10-3), and generates gas by the oxygenation pump (10-4) to oxygenate and aerate the water in the mobile biological treatment tank (10) through the oxygenation aeration pipe (10-5); water in a mobile biological treatment tank (10) enters a fixed biological treatment tank (13) through a first filter screen (10-2) and a connecting pipe (12) and passes through the first filter screen (13-2), ammonia nitrogen, nitrate and nitrite in the water are retreated through a fixed biological filter material (13-3), the treated water flows into a sand filter tank (15) through a second filter screen (13-4) and an overflow pipe (14), the water is uniformly distributed above the sand filter tank (15) through a first water distribution plate (15-1), fine particle suspended matters in the water are filtered by using filter sand (15-2), the water enters a purification tank (16) after passing through the second filter screen (15-3), the water is distributed through a second water distribution plate (16-2), micro impurities in the water are purified and adsorbed by using activated carbon (16-3), and the p H hardness of the water quality is adjusted by using coral stones (16-4), the treated water enters an oxygenation tank (17), and the oxygenation is carried out on the treated water by an oxygenation device to adjust the dissolved oxygen in the water; automatically detecting p H by using a water p H value monitoring detector (18) and controlling the value of the aquaculture water p H; the temperature of the aquaculture water body is automatically detected by using a temperature detection controller (4), and the set water temperature of the aquaculture is controlled to complete the whole aquaculture circulating water treatment process; the adjusted aquaculture water body is pumped away by the circulating pump (1), so that the aquaculture water body enters the next circulating water treatment process.
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