CN109880864A - The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel - Google Patents
The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel Download PDFInfo
- Publication number
- CN109880864A CN109880864A CN201910168780.3A CN201910168780A CN109880864A CN 109880864 A CN109880864 A CN 109880864A CN 201910168780 A CN201910168780 A CN 201910168780A CN 109880864 A CN109880864 A CN 109880864A
- Authority
- CN
- China
- Prior art keywords
- procyanidine
- longan
- enzyme
- supernatant
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides the enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel, belongs to technical field of biological extraction.The present invention passes through hot water Enzymatic Extraction, concentration, stratification, combined extracting function polysaccharide and procyanidine.Method extraction process of the invention is simple and efficient, low in cost, realizes the high-valued utilization of resources of longan peel waste, increases the benefit of longan deep processing, has positive facilitation to the comprehensive exploitation of longan by-product.
Description
Technical field
The invention belongs to technical field of biological extraction, and in particular to function polysaccharide and procyanidine in a kind of longan peel
Enzyme process method for integrated extraction.
Background technique
Longan is a kind of full of nutrition, the fruit of fresh food, processing dual-purpose type.With the continuous improvement of agriculture production technology,
The annual output of longan just grows steadily.Nowadays, longan has been largely used to the processing systems such as canned longan, dried longan of making wine or produce
Product account for the pericarp of 17% or more longan fruit fresh weight, fruit stone is taken as waste to abandon in default of means are effectively utilized.The whole nation
Discarded longan peel, fruit stone are up to 20,000 tons/year or more, it would be highly desirable to comprehensively utilize.Biological active matter is rich in longan peel fruit stone
Matter, especially procyanidine and function polysaccharide.Longan peel procyanidins have very excellent antioxidant activity and α-shallow lake
Powder enzyme inhibition activity.Polysaccharide in longan peel has been proved to possess the bioactivity such as strengthen immunity, antitumor.Exploitation dragon
Physiological activity procyanidine and function polysaccharide in eye pericarp, fruit stone, can turn waste into wealth, and provide new health food or drug money
Source；Its high added value can be excavated again, promote the economic benefit of longan plantation, processing.
Currently, being concentrated mainly on its clear nutritional ingredient or extraction, mirror to the research of longan in terms of food development both at home and abroad
Fixed function factor therein etc., but deep processing limitation.CN201410811029.8 provides a kind of longan peel extract
Extracting method, by longan peel with volumetric concentration be 20-95% ethanol water extract, extracting solution concentration after slightly mentioned
Crude extract is passed through macroporous resin column chromatography by object, and eluent is the ethanol water of volumetric concentration 20-95%, obtains longan fruit
Bark extract does not study its active material specifically although confirming the active value of the extract.
CN201711040011.2 discloses the extraction process of litchi rind procyanidine, comprising the following steps: by litchi rind
It smashs to pieces, ethanol solution is added and extracts, obtains ethanol extract, ethanol extract is filtered, obtain medicinal extract.Body is added into medicinal extract
Product concentration is that 50~70% ethanol solutions adjust litchi rind concentration to 0.3~0.5 mg/ml, and stirring and dissolving, then upper XAD-7 is big
Hole resin is first eluted with distillation washing column, then the ethanol solution for being 70~90% with volumetric concentration, collects efflux and second respectively
Alcohol eluen, then by AB-8+ resin on efflux, first with distillation washing column, then the ethyl alcohol for being 40~50% with volumetric concentration
Solution elution, collects ethanol eluate；Ethanol eluate it will merge twice, filtering, filtrate concentration, freeze-drying, as lichee
Skin procyanidine.The purity is high of procyanidine made from the invention, yield is big, but cumbersome, and extraction of substance is single to be caused greatly
Content of starting materials waste.CN201810051344.3 provides a kind of extracting method of pitaya peel polysaccharide, is soaked using 22-28 DEG C of cold water
The method combined with ethanol precipitation is proposed, 22-28 DEG C of water is added into pitaya peel, is beaten, is sunk using ethyl alcohol
It forms sediment, then using the protein in sevage reagent removal Thick many candies, essence mentions polysaccharide, the pitaya peel polysaccharide that the invention provides
Extracting method is higher to the yield of pitaya peel polysaccharide, but purity is lower.
The present invention provides longan function polysaccharides and the integrated method extracted of the enzyme process of procyanidine.In research something
When chemical composition, procyanidine is usually extracted with organic solvent, and polysaccharide component is extracted with hot water, but lack the former flower of continuous preparation
The related invention of the integrated technology of green element and polysaccharide.Procyanidine usually is esterified to form macromolecular in plant with polysaccharide, causes
It is difficult to directly be extracted with water, but with the progress of bio-enzyme engineering, cellulase, hemicellulase being capable of enzymolysis of plants Central Plains flowers
Green element-polysaccharide composite body.The present invention integrates extraction functionality polysaccharide and inoxidizability procyanidine by enzyme process, not only realizes former
The continuous extraction of anthocyanidin and polysaccharide also while improving procyanidine yield and polysaccharide yield.
Summary of the invention
It is an object of the invention in view of the deficienciess of the prior art, providing function polysaccharide and original in a kind of longan peel
The enzyme process method for integrated extraction of anthocyanidin.Method extraction process of the invention is simple and efficient, realizes the company of procyanidine and polysaccharide
It is continuous to extract, it is low in cost, the higher value application of longan peel waste is realized, the benefit of longan deep processing is increased, to dragon
The comprehensive exploitation of eye by-product has positive facilitation.
To achieve the above object, the present invention adopts the following technical scheme:
The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel, comprising the following steps:
(1) longan peel is ground into peel residue, and complex enzyme solution A is added after sieving with 100 mesh sieve, and hot water enzyme process extracts, later
5000rpm be centrifuged 20 minutes, take supernatant, supernatant A；
(2) supernatant A obtained by step (1) is concentrated, obtains concentrate A, sodium chloride and ethyl alcohol into concentrate A, after stirring and evenly mixing
Stratification, separatory funnel liquid separation, supernatant are procyanidine, and lower layer is Thick many candies solution；
(3) supernatant obtained by step (2) is concentrated, obtains concentrate B, concentrate B is chromatographed with Sephadex column and removed impurity, first used
The elution of 50vol% methanol solution, is eluted with 70vol% acetone soln afterwards, is collected acetone soln eluent revolving, freeze-drying, is obtained
Brown ceramic powder is anti-oxidant procyanidine；
(4) complex enzyme B is added into Thick many candies solution obtained by step (2), albumen is taken off using enzyme process, after the completion of enzymatic hydrolysis, second is added
Alcohol is precipitated, and longan function polysaccharide is lyophilized to obtain in sediment separate out.
In above-mentioned steps (1), complex enzyme A by cellulase, hemicellulase and pectase in 2:1:2 ratio mixing and
At；Complex enzyme solution A is the aqueous solution for the complex enzyme A that concentration is 0.55wt%-0.65wt%.
In above-mentioned steps (1), the liquid-to-solid ratio 20:1 of complex enzyme solution A and peel residue.
In above-mentioned steps (1), the temperature of hot water Enzymatic Extraction is 55 DEG C, extraction time 2.5-3 hour.
Supernatant A is concentrated into the 1/6-1/10 of original volume in above-mentioned steps (2)；1 times of volume is added in concentrate A
95vol% ethyl alcohol and 0.3 times of mass volume ratio of sodium chloride.
Supernatant B is concentrated into the 1/10 of original volume in above-mentioned steps (2).
In above-mentioned steps (4), complex enzyme B is that papain and food flavor enzyme are mixed in the ratio of 4:1；In Thick many candies solution
The complex enzyme B of final concentration of 0.1wt% is added.
In above-mentioned steps (4), 4 times of 95% ethyl alcohol of volume are added and are precipitated.
In above-mentioned steps (4), the Deproteinated hydrolysis temperature of enzyme process is 28-30 DEG C, and enzymolysis time is 2.8-3.0 hours.
Said extracted method prepares resulting function polysaccharide and procyanidine.
The present invention has the advantages that the present invention integrates extraction functionality polysaccharide and anti-oxidant by enzyme process from longan peel
Property procyanidine, not only realize procyanidine and polysaccharide continuous extraction, also at the same improve procyanidine yield and polysaccharide yield.
Method extraction process of the invention is simple and efficient, low in cost, realizes the higher value application of longan peel waste, increases
The benefit of longan deep processing has positive facilitation to the comprehensive exploitation of longan by-product.
Detailed description of the invention
The DPPH free radical scavenging ability of Fig. 1 longan peel procyanidine.
The ABTS free radical scavenging ability of Fig. 2 longan peel procyanidine.
The reducing power of Fig. 3 longan peel procyanidine.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment 1
A kind of enzyme process method for integrated extraction of function polysaccharide and procyanidine in longan peel, comprising the following steps: longan peel
It is ground into peel residue, sieves with 100 mesh sieve rear hot water Enzymatic Extraction, using 0.6wt% complex enzyme A (cellulase/hemicellulase/fruit
Glue enzyme 2:1:2), liquid-to-solid ratio 20:1,55 DEG C extraction time 2.5 hours, be centrifuged (5000rpm, 20 minutes), take supernatant, obtain
Clear liquid A, supernatant A be concentrated into original volume 1/6 concentrate A, into concentrate A be added 0.3 times of (m/v) sodium chloride, 1 times
(v/v) 95vol% ethyl alcohol stirs and evenly mixs rear stratification, separatory funnel liquid separation, and supernatant is procyanidine, and lower layer is thick more
Sugar juice.Supernatant is concentrated into the 1/10 of original volume and obtains concentrate B, concentrate B is chromatographed with Sephadex column and removed impurity, first used
The elution of 50vol% methanol solution, is eluted with 70vol% acetone soln afterwards, collects acetone eluant revolving, freeze-drying, obtained brown
Powder is anti-oxidant procyanidine.Thick many candies solution takes off albumen using enzyme process, and final concentration of 0.1wt% is added in Thick many candies solution
Complex enzyme B(papain/food flavor enzyme 4:1), 30 DEG C of hydrolysis temperature, enzymolysis time 3 hours, after the completion of enzymatic hydrolysis, using 4 times
Longan function polysaccharide is lyophilized to obtain in volume 95vol% ethanol precipitation, sediment separate out.
The yield of preparation gained procyanidine is 0.96%；The yield of function polysaccharide is 2.38%.
Embodiment 2
A kind of enzyme process method for integrated extraction of function polysaccharide and procyanidine in longan peel, comprising the following steps: longan peel
Be ground into peel residue, sieve with 100 mesh sieve rear hot water Enzymatic Extraction, using 0.55wt% complex enzyme A (cellulase/hemicellulase/
Pectase 2:1:2), liquid-to-solid ratio 20:1,55 DEG C extraction time 3 hours, be centrifuged (5000rpm, 20 minutes), take supernatant, obtain
Clear liquid A, supernatant A be concentrated into original volume 1/8 concentrate A, into concentrate A be added 0.3 times of (m/v) sodium chloride, 1 times
(v/v) 95vol% ethyl alcohol stirs and evenly mixs rear stratification, separatory funnel liquid separation, and supernatant is procyanidine, and lower layer is thick more
Sugar juice.Supernatant is concentrated into the 1/10 of original volume and obtains concentrate B, concentrate B is chromatographed with Sephadex column and removed impurity, first used
The elution of 50vol% methanol solution, is eluted with 70vol% acetone soln afterwards, collects acetone eluant revolving, freeze-drying, obtained brown
Powder is anti-oxidant procyanidine.Thick many candies solution takes off albumen using enzyme process, and final concentration of 0.1wt% is added in Thick many candies solution
Complex enzyme B(papain/food flavor enzyme 4:1), 29 DEG C of hydrolysis temperature, enzymolysis time 3.0 hours, after the completion of enzymatic hydrolysis, using 4
Longan function polysaccharide is lyophilized to obtain in times volume 95vol% ethanol precipitation, sediment separate out.
The yield of preparation gained procyanidine is 1.05%；The yield of function polysaccharide is 2.43%.
Embodiment 3
A kind of enzyme process method for integrated extraction of function polysaccharide and procyanidine in longan peel, comprising the following steps: longan peel
It is ground into peel residue, sieves with 100 mesh sieve rear hot water Enzymatic Extraction, using 0.65% complex enzyme A (cellulase/hemicellulase/fruit
Glue enzyme 2:1:2), liquid-to-solid ratio 20:1,55 DEG C extraction time 2.9 hours, be centrifuged (5000rpm, 20 minutes), take supernatant, obtain
Clear liquid A, supernatant A be concentrated into original volume 1/10 concentrate A, into concentrate A be added 0.3 times of (m/v) sodium chloride, 1 times
(v/v) 95vol% ethyl alcohol stirs and evenly mixs rear stratification, separatory funnel liquid separation, and supernatant is procyanidine, and lower layer is thick more
Sugar juice.Supernatant is concentrated into the 1/10 of original volume and obtains concentrate B, concentrate B is chromatographed with Sephadex column and removed impurity, first used
The elution of 50vol% methanol solution, is eluted with 70vol% acetone soln afterwards, collects acetone eluant revolving, freeze-drying, obtained brown
Powder is anti-oxidant procyanidine.Thick many candies solution takes off albumen using enzyme process, and final concentration of 0.1wt% is added in Thick many candies solution
Complex enzyme B(papain/food flavor enzyme 4:1), 30 DEG C of hydrolysis temperature, enzymolysis time 3.0 hours, after the completion of enzymatic hydrolysis, using 4
Longan function polysaccharide is lyophilized to obtain in times volume 95vol% ethanol precipitation, sediment separate out.
The yield of preparation gained procyanidine is 0.98%；The yield of function polysaccharide is 2.42%.
Embodiment 4
A kind of enzyme process method for integrated extraction of function polysaccharide and procyanidine in longan peel, comprising the following steps: longan peel
Be ground into peel residue, sieve with 100 mesh sieve rear hot water Enzymatic Extraction, using 0.55wt% complex enzyme A (cellulase/hemicellulase/
Pectase 2:1:2), liquid-to-solid ratio 20:1,55 DEG C extraction time 2.8 hours, be centrifuged (5000rpm, 20 minutes), take supernatant, obtain
Supernatant A, supernatant A be concentrated into original volume 1/9 concentrate A, into concentrate A be added 0.3 times of (m/v) sodium chloride, 1 times
(v/v) 95vol% ethyl alcohol stirs and evenly mixs rear stratification, separatory funnel liquid separation, and supernatant is procyanidine, and lower layer is thick more
Sugar juice.Supernatant is concentrated into the 1/10 of original volume and obtains concentrate B, concentrate B is chromatographed with Sephadex column and removed impurity, first used
The elution of 50vol% methanol solution, is eluted with 70vol% acetone soln afterwards, collects acetone eluant revolving, freeze-drying, obtained brown
Powder is anti-oxidant procyanidine.Thick many candies solution takes off albumen using enzyme process, and final concentration of 0.1wt% is added in Thick many candies solution
(m/v) complex enzyme B(papain/food flavor enzyme 4:1), 28 DEG C of hydrolysis temperature, enzymolysis time 2.8 hours, after the completion of enzymatic hydrolysis,
Using 4 times of volume 95vol% ethanol precipitations, longan function polysaccharide is lyophilized to obtain in sediment separate out.
The yield of preparation gained procyanidine is 1.02%；The yield of function polysaccharide is 2.36%.
The inoxidizability of 5 longan peel procyanidine of embodiment measures
With the extracted procyanidine of 1 extracting method of embodiment carries out DPPH free-radical scavenging effect, work is quenched in ABTS free radical
Property and reducing power detection, compared with Vc.The DPPH free radical scavenging ability of longan peel procyanidine is shown in Fig. 1, longan peel
The ABTS free radical scavenging ability of procyanidine is shown in that Fig. 2, the reducing power of longan peel procyanidine are shown in Fig. 3.The result shows that DPPH
The IC of the quenching activity of free radical50For 0.083 mg/mL, to the IC of the quenching activity of ABTS free radical50For 0.054 mg/mL,
The IC of reducing power50For 0.061 mg/mL.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (10)
1. the enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel, which is characterized in that including following
Step:
(1) longan peel is ground into peel residue, and complex enzyme solution A is added after sieving with 100 mesh sieve, and the extraction of hot water enzyme process is centrifuged later
Separation, take supernatant, supernatant A；
(2) supernatant A obtained by step (1) is concentrated, obtains concentrate A, sodium chloride and ethyl alcohol into concentrate A, after stirring and evenly mixing
Stratification, separatory funnel liquid separation, supernatant are procyanidine, and lower layer is Thick many candies solution；
(3) supernatant obtained by step (2) is concentrated, obtains concentrate B, concentrate B column chromatography removes impurity, first uses 50vol% first
Alcoholic solution elution, is eluted with 70vol% acetone soln afterwards, collects acetone soln eluent revolving, freeze-drying, obtained brown ceramic powder
For anti-oxidant procyanidine；
(4) complex enzyme B is added into Thick many candies solution obtained by step (2), albumen is taken off using enzyme process, after the completion of enzymatic hydrolysis, second is added
Alcohol is precipitated, and longan function polysaccharide is lyophilized to obtain in sediment separate out.
2. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (1), complex enzyme A by cellulase,
Hemicellulase and pectase are mixed in the ratio of 2:1:2；Complex enzyme solution A is final concentration of 0.55wt%-0.65wt%
Complex enzyme A aqueous solution.
3. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (1), complex enzyme solution A and pericarp
The liquid-to-solid ratio 20:1 of slag.
4. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (1), the temperature of hot water Enzymatic Extraction
It is 55 DEG C, extraction time 2.5-3 hour.
5. extracting method according to claim 1, it is characterised in that: supernatant A is concentrated into original volume in above-mentioned steps (2)
1/6-1/10；The 95vol% ethyl alcohol and mass volume ratio of 1 times of volume are added in concentrate A as 0.3 sodium chloride.
6. extracting method according to claim 1, it is characterised in that: supernatant B is concentrated into original volume in above-mentioned steps (3)
1/10.
7. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (4), complex enzyme B is Papain
Enzyme and food flavor enzyme are mixed in the ratio of 4:1；The complex enzyme B of final concentration of 0.1wt% is added in Thick many candies solution.
8. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (4), the Deproteinated enzymatic hydrolysis temperature of enzyme process
Degree is 28-30 DEG C, and enzymolysis time is 2.8-3.0 hours.
9. extracting method according to claim 1, it is characterised in that: in above-mentioned steps (4), 4 times of volume 95vol% are added
Ethyl alcohol is precipitated.
10. the extracting method as described in claim 1 to 9 prepares resulting function polysaccharide and procyanidine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910168780.3A CN109880864A (en) | 2019-03-06 | 2019-03-06 | The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910168780.3A CN109880864A (en) | 2019-03-06 | 2019-03-06 | The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109880864A true CN109880864A (en) | 2019-06-14 |
Family
ID=66930997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910168780.3A Pending CN109880864A (en) | 2019-03-06 | 2019-03-06 | The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109880864A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114044837A (en) * | 2021-12-03 | 2022-02-15 | 湖南科技学院 | Method for jointly extracting passion fruit anthocyanin and cellulose biomass |
| NL2029007B1 (en) * | 2021-01-21 | 2022-07-28 | Inst Of Animal Science Guangdong Academy Of Agricultural Sciences | Extraction and purification method and application of plant-condensed tannin |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440388A (en) * | 2008-12-30 | 2009-05-27 | 广西医科大学 | Method for extracting, separating and purifying Arillus longan polysaccharide |
| CN102234671A (en) * | 2010-05-07 | 2011-11-09 | 中国农业科学院农产品加工研究所 | Method for extracting polysaccharide from longan pulp |
| CN105030878A (en) * | 2015-08-05 | 2015-11-11 | 张家界立功旅游农业发展有限公司 | Method for extracting multiple active ingredients of blueberry fruits |
| CN106220748A (en) * | 2016-07-30 | 2016-12-14 | 山东胜伟园林科技有限公司 | A kind of Salicornia Bigelovii Torr. total flavones and polysaccharide combined extraction method |
| CN107080778A (en) * | 2017-04-02 | 2017-08-22 | 方家铺子（福建）现代农业发展有限公司 | A kind of multiplex-enzyme extraction technique of longan pulp solid carbon dioxide insoluble active thing and application |
| CN108440480A (en) * | 2018-04-04 | 2018-08-24 | 北京国康本草物种生物科学技术研究院有限公司 | The extracting method of lycium ruthenicum procyanidins and obtained extract |
-
2019
- 2019-03-06 CN CN201910168780.3A patent/CN109880864A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101440388A (en) * | 2008-12-30 | 2009-05-27 | 广西医科大学 | Method for extracting, separating and purifying Arillus longan polysaccharide |
| CN102234671A (en) * | 2010-05-07 | 2011-11-09 | 中国农业科学院农产品加工研究所 | Method for extracting polysaccharide from longan pulp |
| CN105030878A (en) * | 2015-08-05 | 2015-11-11 | 张家界立功旅游农业发展有限公司 | Method for extracting multiple active ingredients of blueberry fruits |
| CN106220748A (en) * | 2016-07-30 | 2016-12-14 | 山东胜伟园林科技有限公司 | A kind of Salicornia Bigelovii Torr. total flavones and polysaccharide combined extraction method |
| CN107080778A (en) * | 2017-04-02 | 2017-08-22 | 方家铺子（福建）现代农业发展有限公司 | A kind of multiplex-enzyme extraction technique of longan pulp solid carbon dioxide insoluble active thing and application |
| CN108440480A (en) * | 2018-04-04 | 2018-08-24 | 北京国康本草物种生物科学技术研究院有限公司 | The extracting method of lycium ruthenicum procyanidins and obtained extract |
Non-Patent Citations (6)
| Title |
|---|
| CAILI FU等: "Structure, antioxidant and α-amylase inhibitory activities of longan pericarp proanthocyanidins", 《SCIENCEDIRECT》 * |
| JINYU CHEN等: "Screening of Key Antioxidant Compounds of Longan (Dimocarpus longan Lour.) Seed Extract by Combining Online Fishing/Knockout, Activity Evaluation, Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, and High-Performance Liquid Chromatography", 《J. AGRIC. FOOD CHEM.》 * |
| YANG YI等: "Immunomodulatory Activity of Polysaccharide-Protein Complex of Longan (Dimocarpus longan Lour.) Pulp", 《MOLECULES》 * |
| 胡宇凌等: "酶促法提取油菜籽皮中的原花青素研究", 《食品与发酵科技》 * |
| 黄尚荣: "龙眼皮渣中原花青素的提取及其活性研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 黄尚荣等: "龙眼皮原花青素提取工艺优化及其抗氧化活性测定", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2029007B1 (en) * | 2021-01-21 | 2022-07-28 | Inst Of Animal Science Guangdong Academy Of Agricultural Sciences | Extraction and purification method and application of plant-condensed tannin |
| CN114044837A (en) * | 2021-12-03 | 2022-02-15 | 湖南科技学院 | Method for jointly extracting passion fruit anthocyanin and cellulose biomass |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105949163B (en) | The method for extraction and purification of anthocyanidin in a kind of Black Box Tracing pomace | |
| CN101942355A (en) | Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds | |
| CN102657331A (en) | Fermented ginseng fermented by bacillus subtilis, fermented ginseng natto and application of extracts | |
| CN105249086B (en) | A kind of preparation process of the red date concentrated juice of high cAMP contents | |
| CN107686473B (en) | A kind of gynura bicolor polysaccharide polyphenol combined preparation process | |
| CN107501365A (en) | A kind of method that raspberry glycosides and anthocyanin are extracted from raspberry | |
| CN106906243A (en) | A kind of method of cordyceps militaris link bacterial strain liquid state fermentation | |
| CN102462701A (en) | Enzyme method for refining Chinese medicine extract | |
| CN107722132B (en) | Method for co-producing algal polysaccharide and algal protein from algae | |
| CN109880864A (en) | The enzyme process method for integrated extraction of function polysaccharide and procyanidine in a kind of longan peel | |
| CN105558191A (en) | Fructus Momordicae and Cyclocarya Paliurus extract mixed tea and preparation method thereof | |
| CN103992876A (en) | Extraction method of passionflower seed oil | |
| CN101225421A (en) | Novel process for extracting golden fungus polysaccharides by step enzyme method | |
| CN110066350A (en) | Blue or green money willow Polyose extraction and the method for blue or green money willow polysaccharide solid beverage preparation | |
| CN108358822B (en) | Method for continuously extracting multiple active ingredients from defective and defective wolfberry fruits | |
| CN104292355B (en) | A kind of extracting method of platycodon root polysaccharide | |
| Yu et al. | A comprehensive review of goji berry processing and utilization | |
| CN103113489B (en) | Method of purifying polysaccharide of Xinjiang jun dates | |
| CN109170885A (en) | A method of extracting ginseng effective component | |
| CN105410279A (en) | Composite tea composed of extracts of siraitia grosvenorii and radix isatidis and preparation method thereof | |
| CN109897119B (en) | Lycium barbarum polysaccharide, method for controlling pH value and concentrating during preparation of lycium barbarum polysaccharide and application of lycium barbarum polysaccharide | |
| JP6944069B2 (en) | How to extract lycopene and citrulline from watermelon at the same time | |
| CN111109511A (en) | Rapid peeling method for food-grade walnut kernels | |
| CN103190632A (en) | Preparation method of edible sea-buckthorn dietary fiber | |
| KR100643735B1 (en) | Fermented Wine made from Fruit of Araliaceous Shrubs, and Method for producing Thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190614 |