CN109874960B - Preparation process of bee pollen crystal - Google Patents
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Abstract
The invention discloses a preparation process of bee pollen crystal, which comprises the following steps: weighing camellia pollen, medlar pollen, motherwort pollen, rose pollen, milk powder, pumpkin powder, cocoa powder, hydrophilic colloid, liquid components and an additive; II, secondly: removing impurities from camellia pollen, fructus Lycii pollen, herba Leonuri pollen, and flos Rosae Rugosae pollen, sterilizing, drying, breaking cell wall, and collecting; thirdly, the method comprises the following steps: extracting the collected wall-broken pollen with ethanol solution, enriching with macroporous resin column, eluting with ethanol, and collecting to obtain mixture A; step four: pulverizing milk powder, fructus Cucurbitae Moschatae powder, and cocoa powder with supersonic airflow pulverizer, and mixing to obtain mixture B; step five: mixing the mixture A and the mixture B, adding hydrophilic colloid, liquid component and additive to prepare loose and crisp granular finished products, and obtaining bee pollen crystal; the pollen crystal prepared by the invention has high nutritive value, and the nutrient components are easy to be absorbed by human body and are convenient to carry and drink.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a preparation process of bee pollen crystal
Background
According to the history, bee pollen refers to pollen formed by storing and fermenting pollen balls brought back by bees during honey collection in a honeycomb. Bee pollen is a male germ cell in stamen of a flowering plant, not only carries genetic information of life, but also contains all nutrient substances necessary for breeding new life, and is the root of the succeeding generation of the plant and a source of heat energy; when bees collect honey, pollen can be collected by the sufficient pollen carrying capacity to form a pollen ball, and the pollen ball can be stored after entering the honeycomb. The main dietotherapy ingredients are as follows: protein, amino acid, vitamin, bee pollen essence, trace elements, active enzyme, flavonoid, lipid, nucleic acid, brassin, phytic acid, etc. The amino acid content and the proportion thereof are the amino acid patterns closest to the food and agriculture organization recommendations of the United nations, which are very rare in natural foods. Bee pollen is a concentrate of nutritionally and pharmacodynamically valuable substances, which contains proteins, carbohydrates, minerals, vitamins and other active substances. Bee pollen is an excellent natural nutritious food, and an ideal tonic, and has certain medical function.
However, the fresh bee pollen is not easy to store after being collected, and can go mouldy and go bad in a short period of time in a high-temperature humid environment, and the content of microorganisms in the bee pollen can be increased after being stored for a long time at normal temperature; in addition, the cellulose content in the pollen wall is high, and the pollen is not easy to dissolve in water, so that the taste of the pollen and the absorption of a human body are influenced.
Disclosure of Invention
Aiming at the technical problems, the invention provides the preparation process of the bee pollen crystal which has simple preparation process, high nutritive value, easy absorption and convenient carrying.
The technical scheme of the invention is as follows: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 28-45 parts of camellia pollen, 5-15 parts of medlar pollen, 8-14 parts of motherwort pollen, 2-3 parts of rose pollen, 10-18 parts of milk powder, 8-15 parts of pumpkin powder, 5-12 parts of cocoa powder, 14-25 parts of hydrophilic colloid, 40-60 parts of liquid component and 0.1-0.2 part of additive;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from any one or any combination of more than two of high fructose corn syrup, beet syrup, isomerized corn syrup or type 50 syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is lower than 3%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 25-36 deg.C, the pressure is controlled at 40-75MPa, the treatment time is 15-30min, then the carbon dioxide in the extraction device is evacuated, and the wall-broken pollen is collected;
step three: extracting the wall-broken pollen collected in the second step with ethanol solution respectively to obtain ethanol extract, then enriching the ethanol extract with a nonpolar macroporous resin column, eluting with ethanol, collecting ethanol elution parts, concentrating, drying, and mixing to obtain mixed extract A for later use;
step four: respectively pulverizing the milk powder, the pumpkin powder and the cocoa powder in the step one into superfine powder with the particle size less than 10 microns by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the additive, uniformly stirring, preparing into particles with the particle size of 20-25 meshes by using a granulator, and finally freeze-drying at the temperature of-10-50 ℃ for 120-150 minutes to prepare loose and crisp granular finished products, namely the bee pollen crystal.
Further, in the step one, the additive is CBD, and the CBD has the effects of improving sleep quality and relaxing the body and mind.
Further, in the second step, 0.03-0.06 part of wall-breaking enzyme is added into the supercritical carbon dioxide extraction device, and the wall-breaking enzyme is any one or a mixture of any two or three of glycosidase, protease and peptidase; glycosidase, protease and peptidase can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and have high efficiency.
Further, in the third step, when the wall-broken pollen is extracted by ethanol solution, the wall-broken pollen is taken and added into 9-20 times of 45-60% ethanol water solution by volume fraction, and is soaked for 3-8 days at the temperature of 18-42 ℃, and the ethanol extract is obtained after filtration;
further, in the third step, the specific operation of enriching the effective components in the wall-broken pollen by the nonpolar macroporous resin column is as follows: loading the ethanol extract into a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 30-85% ethanol aqueous solution by volume fraction, concentrating, drying, and mixing to obtain a mixed extract A.
Further, in the fifth step, in the granulating process, 0.15-0.2 part of binder is added into the granulator, and the binder is prepared by mixing absolute ethyl alcohol and purified water according to the proportion of 1: 3.
Furthermore, in the fourth step, the vertical multi-screw continuous mixing device comprises a support frame, a mixing bin, a mixer and a blanking bin; the blending bunker sets up at the support frame lower extreme, the blending bunker runs through the up end of support frame, the blending bunker lower extreme is provided with collects the mouth, the blender activity sets up inside the blending bunker, the blanking storehouse has threely, three blanking storehouse evenly sets up the up end at the support frame, and the blanking mouth and the blending bunker of three blanking storehouse link up, glassware under three blanking storehouse inside all is provided with the spiral, all be provided with feeding regulation picture peg on the blanking mouth of three blanking storehouse, three blanking storehouse is used for placing milk powder respectively, pumpkin powder and cocoa powder, glassware is supplied power by external power source under blender and the spiral.
Further, before the second step, injecting 45ug/ml ozone into purified water, respectively adding camellia pollen, lycium barbarum pollen and leonurus japonicus pollen into the purified water according to the mass ratio of 1:3, and uniformly stirring to obtain pollen liquid; then filtering the pollen liquid by using a filter to obtain filtered pollen liquid; and finally, separating the filtered pollen liquid by using a spiral discharge sedimentation centrifuge, and discharging the separated sewage to obtain pure pollen.
Compared with the prior art, the invention has the beneficial effects that: the invention prepares the pollen crystal by processing the fresh pollen after breaking the wall and then adding other auxiliary materials, thereby not only having high nutritive value and easily absorbing the nutrient components by the human body, but also being convenient for carrying and drinking; the addition of the wall-breaking enzyme greatly improves the pollen wall-breaking rate of the fermentation enzymatic wall-breaking method, has small nutrient loss and good activity preservation; no food additive is added in the preparation process of the pollen crystal, so that the food safety is improved; the effective components in the wall-broken pollen are enriched by using a nonpolar macroporous resin column, the nonpolar macroporous resin column is eluted by using an ethanol solution, the effective components in the wall-broken pollen are collected, the loss of the nutrient components in the pollen is reduced to a limited extent, and the nutrient value of the pollen crystal is improved.
Drawings
FIG. 1 is a schematic structural view of a vertical multi-screw continuous mixing apparatus of the present invention;
FIG. 2 is a top view of the vertical multi-screw continuous mixing apparatus of the present invention;
wherein, the device comprises a support frame 1, a mixing bin 2, a collecting port 20, a mixer 3, a blanking bin 4, a spiral blanking device 40 and a feeding adjusting inserting plate 41.
The main functions of the raw materials of the invention are as follows:
camellia powder: the efficacy of the camellia pollen comes from the inherent charm and drug effect of the camellia pollen, and the camellia pollen can maintain good metabolism and keep skin luster. The tea pollen has the effect of ensuring sufficient vitamin b in human body, wherein the vitamin b is water-soluble vitamin, regulating metabolism, maintaining health of skin and muscle, enhancing functions of immune system and nervous system, and promoting cell growth and division. The efficacy of camellia pollen can also be that you are younger and more beautiful to let you feel confident; the camellia pollen is rich in a plurality of effective active ingredients such as protein, amino acid, fatty acid, vitamin, active enzyme and the like, wherein the content of the protein and the amino acid is 5-7 times of that of the eggs and the milk, and the camellia pollen is called as complete protein nutritionally; the unsaturated fatty acid in the fatty acid is far higher than that of other animals and plants, and some of the unsaturated fatty acids are essential fatty acids which cannot be synthesized by a human body; the vitamin content is the most with b vitamins, and the camellia pollen can convert fat into energy and utilize the energy; camellia pollen is the king of pollen; the camellia pollen has the advantages of unique effects on beautifying, reducing fat, removing chloasma and acne, delaying senility, orange red color, fragrant smell, slight sweetness and delicious taste, and high contents of amino acid, vitamin and trace elements, wherein the amino acid content is the first of pollen, and the contents of the trace elements of manganese, cobalt, chromium and nicotinic acid are higher than those of common pollen. Therefore, pollen is the first choice for health care and medical treatment. The pollen has good therapeutic effect on heart disease, diabetes, hepatitis, constipation, dysentery, enteritis, dysuresia, edema, neurasthenia, prostatitis, etc.; can also be used for preventing and treating tumor, arteriosclerosis, senile dementia, children mental retardation, endocrine disturbance, etc.; it is effective in preventing and treating arteriosclerosis, enhancing myocardial contraction, increasing heart rate, and improving myocardial function.
Medlar pollen: the medlar pollen has complex components and mainly comprises 20 to 25 percent of protein, more than 20 percent of total amino acid, 1 to 2 percent of free amino acid, 40 to 50 percent of carbohydrate and 5 to 10 percent of lipid; the nutrient wine also contains various nutrient substances such as abundant vitamins, trace elements, enzymes, nucleic acid, hormone and the like, more than 200 nutrient components separated by scientific research are available at present, and a plurality of effective components are not recognized by people. The medlar contains rich carotene, vitamin B1, vitamin B2, nicotinic acid, vitamin C, vitamin E, a plurality of amino acids, linoleic acid, betaine, ferrum, potassium, zinc, calcium, phosphorus, selenium and other skin beautifying and moisturizing components; the contained abundant carotene, vitamin B1, vitamin B2, vitamin C, calcium, iron and the like are essential nutrients for healthy eyes; contains various unsaturated fatty acids of betaine, and the betaine can inhibit fat deposition in liver, prevent liver cirrhosis, and protect normal liver cells; the medlar also has certain effect on reducing blood fat and blood sugar; the fructus Lycii has effects of enhancing immunity, promoting cell regeneration, reducing blood cholesterol content, resisting arteriosclerosis, improving skin elasticity, resisting organ and skin aging, and delaying skin wrinkle. The medlar is a health food which is worth eating in large quantity, can nourish yin, improve eyesight, tonify kidney and strengthen body, and can strengthen bones and muscles to cure rheumatalgia by taking the medlar frequently; but also can improve blood circulation and improve body adaptability. Moreover, the medlar is the most excellent curative effect for people, and has the effects of whitening skin, improving eyesight and calming the nerves after being taken frequently, even being considered as being capable of prolonging life and lightening the body. Medlar is bitter in taste and cold in nature, has the effect of cooling blood, and can play a role in treatment if the medlar is decocted for taking if the medlar has cough with lung heat and even bloody sputum.
Motherwort pollen: motherwort pollen is the essence collected on stamens of flowers such as various flowers, grasses, overuse eyes and medicinal materials, is also named as "Baihuajing", and is a valuable natural medicinal material. Pollen of different plants also has different colors. Slightly sweet and bitter in taste. The bee pollen is a substance with high activity and high quality, the components of the pollen which is not broken are not easy to be damaged and easy to be absorbed by human body, and the quality is better than that of the broken pollen. Is a good beauty and health care product with rich nutrition and charming price. Bee pollen contains various nutrient substances and abundant bioactive substances required by organisms, and has strong functions of resisting aging, strengthening physique, eliminating fatigue and improving immunity of the organisms. Pollen is often eaten by many athletes abroad, and is formally determined as a health food for athletes in China by national physical committees.
Detailed Description
Example 1: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 28 parts of camellia pollen, 5 parts of medlar pollen, 8 parts of motherwort pollen, 2 parts of rose pollen, 10 parts of milk powder, 8 parts of pumpkin powder, 5 parts of cocoa powder, 14 parts of hydrophilic colloid, 40 parts of liquid component and 0.1 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from high fructose corn syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is 2%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 25 ℃, the pressure is controlled at 40MPa, the treatment time is 15min, then the carbon dioxide in the extraction device is emptied, and the wall-broken pollen is collected;
step three: respectively extracting the wall-broken pollen collected in the second step with ethanol solution, adding 9 times of 45% ethanol water solution, soaking at 18 deg.C for 3 days, and filtering to obtain ethanol extract; loading the ethanol extract into a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 30% ethanol aqueous solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain a mixed extract A;
step four: respectively pulverizing the milk powder, pumpkin powder and cocoa powder in the step one into ultrafine powder with the particle size of 9 microns by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding hydrophilic colloid, liquid component and additive, stirring, granulating into 20 mesh granules with a granulator, and freeze drying at-50 deg.C for 120 min to obtain loose and crisp granular product, i.e. bee pollen crystal.
Example 2: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 36 parts of camellia pollen, 9 parts of medlar pollen, 10 parts of motherwort pollen, 2 parts of rose pollen, 15 parts of milk powder, 13 parts of pumpkin powder, 9 parts of cocoa powder, 18 parts of hydrophilic colloid, 45 parts of liquid component and 0.15 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind;
the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from beet syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the water content is 1%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 30 ℃, the pressure is 58MPa, the processing time is 22min, then the carbon dioxide in the extraction device is emptied, the wall-broken pollen is collected, 0.05 part of wall-breaking enzyme is added into the supercritical carbon dioxide extraction device, and the wall-breaking enzyme is glycosidase; the glycosidase can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and has high efficiency;
step three: extracting the wall-broken pollen collected in the second step with ethanol solution respectively, adding 15 times of 52% ethanol water solution, soaking at 31 deg.C for 6 days, and filtering to obtain ethanol extract; loading the ethanol extract into a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 56% ethanol aqueous solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain a mixed extract A;
step four: respectively pulverizing the milk powder, pumpkin powder and cocoa powder in the step one into 7-micron ultrafine powder by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the above hydrophilic colloid, liquid components and additives, stirring, granulating into 23 mesh granules with a granulator, and freeze drying at-35 deg.C for 136 min to obtain loose and crisp granular product, i.e. bee pollen crystal.
Example 3: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 45 parts of camellia pollen, 15 parts of medlar pollen, 14 parts of motherwort pollen, 3 parts of rose pollen, 18 parts of milk powder, 15 parts of pumpkin powder, 12 parts of cocoa powder, 19 parts of hydrophilic colloid, 60 parts of liquid component and 0.2 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind;
the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from isomerized corn syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is 2%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 36 ℃, the pressure is controlled at 75MPa, the treatment time is 30min, then the carbon dioxide in the extraction device is emptied, the wall-broken pollen is collected, 0.06 part of wall-breaking enzyme is added into the supercritical carbon dioxide extraction device, and the wall-breaking enzyme is a mixture of glycosidase and protease; glycosidase and protease can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and the method has high efficiency;
step three: extracting the wall-broken pollen collected in the second step with ethanol solution respectively, adding 20 times of 60% ethanol water solution, soaking at 42 deg.C for 8 days, and filtering to obtain ethanol extract; loading the ethanol extract onto a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 85% ethanol water solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain mixed extract A;
step four: respectively pulverizing the milk powder, pumpkin powder and cocoa powder in the step one into ultrafine powder with the particle size of 8 microns by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the additive, uniformly stirring, and preparing into granules with the particle size of 25 meshes by using a granulator, wherein in the granulating process, 0.2 part of binder is added into the granulator, and the binder is prepared by mixing absolute ethyl alcohol and purified water according to the proportion of 1: 3; and finally, freeze-drying for 150 minutes at the temperature of-25 ℃ to prepare a loose and crisp granular finished product 3 percent, namely the bee pollen crystal.
Example 4: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 28 parts of camellia pollen, 5 parts of medlar pollen, 8 parts of motherwort pollen, 2 parts of rose pollen, 10 parts of milk powder, 8 parts of pumpkin powder, 5 parts of cocoa powder, 19 parts of hydrophilic colloid, 50 parts of liquid component and 0.2 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from high 50 type syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is 2%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 25 ℃, the pressure is controlled at 40MPa, the processing time is 15min, then the carbon dioxide in the extraction device is emptied, the wall-broken pollen is collected, 0.03 part of wall-breaking enzyme is added into the supercritical carbon dioxide extraction device, and the wall-breaking enzyme is a mixture of glycosidase, protease and peptidase; glycosidase, protease and peptidase can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and have high efficiency;
step three: respectively extracting the wall-broken pollen collected in the second step with ethanol solution, adding 9 times of 45% ethanol water solution, soaking at 18 deg.C for 3 days, and filtering to obtain ethanol extract; loading the ethanol extract into a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 30% ethanol aqueous solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain a mixed extract A;
step four: respectively crushing the milk powder, the pumpkin powder and the cocoa powder in the step one into superfine powder with the particle size of 6 microns by a supersonic airflow crusher, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the additive, uniformly stirring, and preparing into granules with the particle size of 20 meshes by using a granulator, wherein in the granulating process, 0.15 part of binder is added into the granulator, and the binder is prepared by mixing absolute ethyl alcohol and purified water according to the proportion of 1: 3; and finally, freeze-drying at the temperature of-15 ℃ for 120 minutes to prepare a loose and crisp granular finished product, namely the bee pollen crystal.
Example 5: a preparation process of bee pollen crystal comprises the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 45 parts of camellia pollen, 15 parts of medlar pollen, 14 parts of motherwort pollen, 3 parts of rose pollen, 18 parts of milk powder, 15 parts of pumpkin powder, 12 parts of cocoa powder, 22 parts of hydrophilic colloid, 55 parts of liquid component and 0.2 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind; injecting 45ug/ml ozone into purified water, adding camellia pollen, medlar pollen and motherwort pollen into the purified water according to the mass ratio of 1:3, and stirring uniformly to obtain pollen liquid; then filtering the pollen liquid by using a filter to obtain filtered pollen liquid; finally, separating the filtered pollen liquid by using a spiral discharge sedimentation centrifuge, and discharging the separated sewage to obtain pure pollen; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from the group consisting of high fructose corn syrup, sugar beet syrup, isomerized corn syrup, and type 50 syrup;
step two: removing impurities of the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is 2%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 36 ℃, the pressure is controlled at 75MPa, the treatment time is 30min, then the carbon dioxide in the extraction device is emptied, the wall-broken pollen is collected, 0.06 part of wall-broken enzyme is added into the supercritical carbon dioxide extraction device, and the wall-broken enzyme is a mixture of glycosidase, protease and peptidase; glycosidase, protease and peptidase can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and have high efficiency;
step three: extracting the wall-broken pollen collected in the second step with ethanol solution respectively, adding 20 times of 60% ethanol water solution, soaking at 42 deg.C for 8 days, and filtering to obtain ethanol extract; loading the ethanol extract onto a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 85% ethanol water solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain mixed extract A;
step four: respectively pulverizing the milk powder, pumpkin powder and cocoa powder in the step one into 5-micron ultrafine powder by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the additive, uniformly stirring, and preparing into granules with the particle size of 25 meshes by using a granulator, wherein in the granulating process, 0.2 part of binder is added into the granulator, and the binder is prepared by mixing absolute ethyl alcohol and purified water according to the proportion of 1: 3; and finally, freeze-drying for 150 minutes at the temperature of 40 ℃ to prepare a loose and crisp granular finished product, namely the bee pollen crystal.
Example 6: a preparation process of bee pollen crystal comprises the following steps: the method comprises the following steps: weighing the following components in parts by weight: 45 parts of camellia pollen, 15 parts of medlar pollen, 14 parts of motherwort pollen, 3 parts of rose pollen, 18 parts of milk powder, 15 parts of pumpkin powder, 12 parts of cocoa powder, 18 parts of hydrophilic colloid, 55 parts of liquid component and 0.1 part of additive; wherein the additive is CBD, and CBD has effects of improving sleep quality and relaxing body and mind; injecting 45ug/ml ozone into purified water, adding camellia pollen, medlar pollen and motherwort pollen into the purified water according to the mass ratio of 1:3, and stirring uniformly to obtain pollen liquid; then filtering the pollen liquid by using a filter to obtain filtered pollen liquid; finally, separating the filtered pollen liquid by using a spiral discharge sedimentation centrifuge, and discharging the separated sewage to obtain pure pollen;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from the group consisting of high fructose corn syrup, sugar beet syrup, isomerized corn syrup, and type 50 syrup;
step two: removing impurities from the camellia pollen, the medlar pollen and the motherwort pollen respectively, sterilizing and drying by using a microwave device until the water content is 2%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 36 ℃, the pressure is controlled at 75MPa, the treatment time is 30min, then the carbon dioxide in the extraction device is emptied, the wall-broken pollen is collected, 0.06 part of wall-broken enzyme is added into the supercritical carbon dioxide extraction device, and the wall-broken enzyme is a mixture of glycosidase, protease and peptidase; glycosidase, protease and peptidase can act on corresponding macromolecular substances on the surface of the pollen wall and at the germination holes to promote the opening of the germination holes and the release of contents, and have high efficiency;
step three: extracting the wall-broken pollen collected in the second step with ethanol solution respectively, adding 20 times of 60% ethanol water solution, soaking at 42 deg.C for 8 days, and filtering to obtain ethanol extract; loading the ethanol extract onto a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 85% ethanol water solution by volume fraction, collecting ethanol eluate, concentrating, drying, and mixing to obtain mixed extract A;
step four: respectively pulverizing the milk powder, pumpkin powder and cocoa powder in the step one into 6-micron ultrafine powder by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use; the vertical multi-screw continuous mixing device comprises a support frame 1, a mixing bin 2, a mixer 3 and a blanking bin 4; the mixing bin 2 is arranged at the lower end of the support frame 1, the mixing bin 2 penetrates through the upper end face of the support frame 1, the lower end of the mixing bin 2 is provided with a collection port 20, the mixer 3 is movably arranged inside the mixing bin 2, three blanking bins 4 are provided, the three blanking bins 4 are uniformly arranged on the upper end face of the support frame 1, blanking ports of the three blanking bins 4 are communicated with the mixing bin 2, spiral blanking devices 40 are arranged inside the three blanking bins 4, feeding adjusting plugboards 41 are arranged on the blanking ports of the three blanking bins 4, the three blanking bins 4 are respectively used for placing milk powder, pumpkin powder and cocoa powder, and the mixer 3 and the spiral blanking devices 40 are powered by an external power supply;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the additive, uniformly stirring, and preparing into granules with the particle size of 25 meshes by using a granulator, wherein in the granulating process, 0.2 part of binder is added into the granulator, and the binder is prepared by mixing absolute ethyl alcohol and purified water according to the proportion of 1: 3; and finally, freeze-drying for 150 minutes at the temperature of 40 ℃ to prepare a loose and crisp granular finished product, namely the bee pollen crystal.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A preparation process of bee pollen crystal is characterized by comprising the following steps:
the method comprises the following steps: weighing the following components in parts by weight: 28-45 parts of camellia pollen, 5-15 parts of medlar pollen, 8-14 parts of motherwort pollen, 2-3 parts of rose pollen, 10-18 parts of milk powder, 8-15 parts of pumpkin powder, 5-12 parts of cocoa powder, 14-25 parts of hydrophilic colloid, 40-60 parts of liquid component and 0.1-0.2 part of additive;
wherein the hydrocolloid is selected from locust bean gum, carrageenan, gum arabic, guar gum, pectin and agar or a combination of these components; the liquid component is a mixture of water, milk and syrup according to a certain proportion; the syrup is selected from any one or any combination of more than two of high fructose corn syrup, beet syrup, isomerized corn syrup or type 50 syrup;
step two: removing impurities from the camellia pollen, the medlar pollen, the motherwort pollen and the rose pollen respectively, sterilizing and drying by using a microwave device until the moisture content is lower than 3%, and then putting the dried pollen into a supercritical carbon dioxide extraction device for wall breaking; wherein the temperature of carbon dioxide in the extraction device is controlled at 25-36 deg.C, the pressure is controlled at 40-75MPa, the treatment time is 15-30min, then the carbon dioxide in the extraction device is evacuated, and the wall-broken pollen is collected;
step three: respectively extracting the wall-broken pollen collected in the second step by using ethanol solution to obtain ethanol extract, then enriching the ethanol extract by using a nonpolar macroporous resin column, eluting by using ethanol, collecting ethanol elution parts, concentrating, drying and mixing to obtain mixed extract A for later use;
step four: respectively pulverizing the milk powder, the pumpkin powder and the cocoa powder in the step one into superfine powder with the particle size less than 10 microns by a supersonic airflow pulverizer, and uniformly mixing by a vertical multi-screw continuous mixing device to obtain a mixture B for later use;
step five: mixing the mixed extract A and the mixture B, adding the hydrophilic colloid, the liquid component and the admixture, uniformly stirring, preparing into particles with the particle size of 20-25 meshes by using a granulator, and finally freeze-drying at the temperature of-10-50 ℃ for 150 minutes to prepare loose and crisp granular finished products, namely the bee pollen crystals;
in the second step, 0.03-0.06 part of wall-breaking enzyme is added into the supercritical carbon dioxide extraction device, wherein the wall-breaking enzyme is any one or a mixture of any two or three of glycosidase, protease and peptidase;
in the third step, when the wall-broken pollen is extracted by using an ethanol solution, the wall-broken pollen is taken and added into 9-20 times of 45-60% ethanol water solution by volume fraction, and is soaked for 3-8 days at the temperature of 18-42 ℃, and the ethanol extract is obtained after filtration;
in the third step, the specific operation of enriching the effective components in the wall-broken pollen by the nonpolar macroporous resin column is as follows: loading the ethanol extract into a nonpolar macroporous resin column, eluting with water to remove impurities, eluting the nonpolar macroporous resin column with 30-85% ethanol aqueous solution by volume fraction, concentrating, drying, and mixing to obtain a mixed extract A.
2. The process of claim 1, wherein in step one, the additive is CBD.
3. The process of claim 1, wherein in step five, 0.15-0.2 part of binder is added into the granulator during granulation, and the binder is prepared by mixing absolute ethyl alcohol and purified water at a ratio of 1: 3.
4. The preparation process of bee pollen crystal as claimed in claim 1, wherein in the fourth step, the vertical multi-screw continuous mixing device comprises a support frame (1), a mixing bin (2), a mixer (3) and a dropping bin (4); mixing bunker (2) sets up at support frame (1) lower extreme, and mixing bunker (2) runs through the up end of support frame (1), and mixing bunker (2) lower extreme is provided with collects mouth (20), blender (3) activity sets up inside mixing bunker (2), blanking storehouse (4) have threely, and three blanking storehouse (4) evenly set up the up end at support frame (1), and the blanking mouth of three blanking storehouse (4) link up with mixing bunker (2), and three blanking storehouse (4) are inside all to be provided with under the spiral glassware (40), all are provided with feeding regulation picture peg (41) on the blanking mouth of three blanking storehouse (4), and three blanking storehouse (4) are used for placing milk powder, pumpkin powder and cocoa powder respectively, and glassware (40) are supplied power by external power source under blender (3) and the spiral.
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