CN109874638B - Novel functional matrix and preparation method thereof - Google Patents

Novel functional matrix and preparation method thereof Download PDF

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CN109874638B
CN109874638B CN201910338459.5A CN201910338459A CN109874638B CN 109874638 B CN109874638 B CN 109874638B CN 201910338459 A CN201910338459 A CN 201910338459A CN 109874638 B CN109874638 B CN 109874638B
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brassinolide
cytokinin
putrescine
matrix
concentration
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CN109874638A (en
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蒋卫杰
余宏军
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Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
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Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
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Abstract

The invention discloses a novel functionThe matrix is prepared by adding putrescine, cytokinin and brassinolide as additives into coconut husk matrix, wherein the concentration of putrescine is 3-7 mmol.L‑1The concentration of the cytokinin is 1 x 10‑2‑3×10‑2mg·L‑1The concentration of the brassinolide is 8 multiplied by 10‑4‑1.2×10‑3mg·L‑1(ii) a A method for preparing a novel functional matrix comprises mixing putrescine, cytokinin and brassinolide, and adding the uniformly mixed putrescine, cytokinin and brassinolide into coconut husk matrix. The novel functional matrix effectively improves the strong seedling rate and the root activity of plants and enhances the crop productivity; the method meets the growth requirement of plants, and has the advantages of consistent growth of the plants, less plant diseases and insect pests, excellent overall character, high comprehensive evaluation index of plant morphology indexes, good commodity quality and high yield.

Description

Novel functional matrix and preparation method thereof
Technical Field
The invention belongs to the field of soilless culture, and particularly relates to a novel functional matrix prepared by using a mixed additive.
Background
At present, the substrate raw materials are various, but the most used contemporary peat with the best quality is added with a small amount of biomass fermentation products and inorganic materials such as vermiculite, perlite and the like. Of these, inorganic materials are produced in China to meet market supply, while peat is mainly imported.
However, peat formation is extremely slow and requires several geological times. Meanwhile, peat lands are a special wetland type, and breed a large amount of organisms, and since the last century, due to the large demand of agriculture and industry for peat resources, the shortage of peat resources is caused, and the environment of a large amount of peat wetlands is damaged.
The extensive exploitation and use of peat causes the destruction of the ecosystem and causes severe environmental and resource pressures. The selection of new functional media for replacing peat has become an important issue to be solved urgently in horticulture.
When the existing coconut husk substrate is used as a seedling substrate, the growth promoting effect on crops is low; and part of the coconut husk matrix contains more components, is complex to prepare and has higher cost.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a novel functional matrix and a preparation method thereof, which avoid the defect that peat is used as the matrix to cause damage to an ecological system and solve the problems of low growth promotion effect, more components, complex preparation and high cost of the prior coconut husk matrix on crops.
In order to achieve the purpose, the novel functional matrix provided by the invention is prepared by adding additives of putrescine, cytokinin and brassinolide into a coconut husk matrix.
Further, the concentration of the putrescine is 3-7 mmol.L-1The concentration of the cytokinin is 1 x 10-2-3×10-2mg·L-1The concentration of the brassinolide is 8 multiplied by 10-4-1.2×10-3mg·L-1
Preferably, the concentration of the putrescine is 5 mmol.L-1The concentration of the cytokinin is 2 x 10-2mg·L-1The concentration of the brassinolide is 10-3mg·L-1
The invention also provides a preparation method of the novel functional matrix, which comprises the steps of mixing the putrescine, the cytokinin and the brassinolide, and adding the uniformly mixed putrescine, cytokinin and brassinolide into the coconut husk matrix.
The novel functional matrix and the preparation method thereof provided by the invention have the following beneficial effects:
1. the growth of cucumber leaves and the accumulation of underground substances can be promoted, the strong seedling rate of cucumber plants reaches 48 after 30 days of field planting, and the root system vitality value reaches 410 mu g/g (FW)/h; effectively improve the strong seedling rate and the root activity of plants and enhance the crop productivity
2. The plant growth requirement is met, the plants grow consistently, the plant diseases and insect pests are few, the overall character is excellent, the comprehensive evaluation index of the plant morphology indexes is high, the commodity quality is good, and the yield is high;
3. relieving the use pressure of the peat and improving the economic benefit and the environmental benefit.
Drawings
FIG. 1 is a graph showing the effect of the coconut husk substrate containing different additives on the leaf area of cucumber plants in example 1.
FIG. 2 is a graph showing the effect of the coconut husk base containing different additives on the fresh weight of cucumber plant roots in example 1.
FIG. 3 is a graph showing the effect of the novel functional matrix of the mixed additives of different concentrations on the strong seedling rate of cucumber plants in example 2.
Fig. 4 is a graph showing the effect of the novel functional substrate of the mixed additive of different concentrations on the root activity of cucumber plants in example 2.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments in order to make the technical field better understand the scheme of the present invention.
A novel functional matrix is prepared by mixing three additives, namely putrescine, cytokinin and brassinolide uniformly, and adding into coconut husk matrix for promoting crop growth; wherein the final concentration of the three additives in the novel functional matrix is 3-7 mmol.L of putrescine-1Cytokinin 1X 10-2-3×10-2mg·L-1Brassinolide 8X 10-4-1.2×10-3mg·L-1
For the catalyst containing 3-7 mmol. multidot.L-1Putrescine, 1X 10-2-3×10-2mg·L-1Cytokinin, 8X 10-4-1.2×10- 3mg·L-1The novel functional matrix of the three additives of the brassinolide is a crop culture matrix, is applied to soilless culture of plants, only contains three additives of putrescine, brassinolide and cytokinin, limits the concentration of the three additives, is simple in ingredient, less in additive, reduces cost and operation time, effectively improves the seedling strengthening rate and root activity of plants, and enhances the crop vitalityAnd (4) capacity. Compared with the prior common coconut chaff or peat used as a culture medium for soilless culture, the novel functional medium screened by the method can meet the requirements of plant growth, and has the advantages of consistent plant growth, less plant diseases and insect pests, excellent overall character, high comprehensive evaluation index of plant morphology indexes, good commodity quality and high yield. The pressure of the peat is relieved to a great extent, and the economic benefit and the environmental benefit are improved.
The invention further provides the application of the novel functional substrate in soilless culture of plants, wherein the plants comprise but are not limited to cucumbers. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The starting materials used in the examples are all commercially available products unless otherwise specified.
Example 1 application of coconut husk base with different additives in cucumber cultivation
15 different additives were added to a coconut coir substrate promoting the growth of crops for the cultivation of cucumber, the obtained substrate being as shown in table 1, ready for use.
TABLE 1 base formulation for different additives to coconut coir
Figure BDA0002039885290000031
Figure BDA0002039885290000041
Cultivation in a greenhouse ensures that the test material is provided with a relatively stable and suitable growth environment having a night temperature of not less than 15 ℃ and a day temperature of not more than 28 ℃ during the test period. Cucumber plants are planted in a coconut husk matrix which promotes the growth of crops by adding 15 different additives, the result of the leaf area of the cucumber plant 30 days after planting is shown in figure 1, and the result of the fresh weight of the root of the cucumber plant 30 days after planting is shown in figure 2.
The results show that: so as to contain 10 mmol. L-1Putrescine coconut husk containing 2 × 10-2mg·L-1Cytokinin coconut husk and coconut husk containing 10- 3mg·L-1The brassinolide coconut chaff can promote the growth of cucumber leaves and the accumulation of underground substances when being used as a cucumber culture medium.
Example 2 application of substrates with mixed additives of different concentrations in cucumber cultivation
The three mixed additives of 27 different concentration combinations are added into coconut chaff for promoting the growth of crops and are used for cultivating cucumbers, and the obtained substrate is shown in table 2 and is ready for use.
TABLE 2 novel functional matrix formulation with various combinations of concentrations of mixed additives added to coconut coir
Figure BDA0002039885290000042
Figure BDA0002039885290000051
Figure BDA0002039885290000061
The test materials are cultivated under the greenhouse condition, the greenhouse is provided with full-automatic temperature control and ventilation facilities, and a relatively stable and suitable growth environment with the night temperature not lower than 15 ℃ and the day temperature not higher than 28 ℃ can be guaranteed to be provided for the test materials during the test period. Cucumber plants are fixedly planted in 27 kinds of mixed additives with different concentration combinations, wherein the three kinds of mixed additives are added into a coconut chaff matrix for promoting the growth of crops, the result of the strong seedling rate of the cucumber plants 30 days after the permanent planting is shown in figure 3, and the result of the root system activity of the cucumber plants 30 days after the permanent planting is shown in figure 4; the plant height, plant height variation, soluble solids of cucumber plants 30 days after planting, blast index of cucumber plants 60 days after planting and yield at 65 days after harvest time are shown in table 3.
The results show that: as shown in fig. 3, the cucumber seedling strengthening rate of the cucumber prepared in the formula 18 is up to 48, which is improved by about four times compared with the formula 1 of the control, and is significantly higher than that of other treatments.
As shown in figure 4, the root system vitality value of the cucumber prepared by 18 reaches 410 mug/g (FW)/h, is improved by 2.85 times compared with the control prepared by 1, and is obviously higher than that of other treatments.
As shown in Table 3, the cucumber plant prepared by 18 has higher growth height than other preparation groups, small difference of plant height, consistent growth, low wilt index, less plant diseases and insect pests, high soluble solid content, excellent overall character, high comprehensive evaluation index of plant morphology indexes, good commodity quality, high yield of 3.4 kg/plant in 65 days of harvest time and high yield.
TABLE 3 Effect of cucumber planting on plant height, disease incidence index, quality and yield
Figure BDA0002039885290000071
Figure BDA0002039885290000081
Therefore, the concentration of the three additives in the novel functional matrix is 5 mmol.L of putrescine-1Cytokinin 2X 10- 2mg·L-1Brassinolide 10-3mg·L-1The method can meet the growth requirement of plants, the strong seedling rate and root activity value of the planted crops are highest, the strong seedling rate and root activity of the plants are effectively improved, and the crop yield is enhanced; and the plants of the planted crops grow consistently, the plant diseases and insect pests are few, the overall character is excellent, the comprehensive evaluation index of the plant morphology indexes is high, the commodity quality is good, and the yield is high.
The inventive concept is explained in detail herein using specific examples, which are given only to aid in understanding the core concepts of the invention. It should be understood that any obvious modifications, equivalents and other improvements made by those skilled in the art without departing from the spirit of the present invention are included in the scope of the present invention.

Claims (3)

1. A novel functional matrix is characterized in thatAdding putrescine, cytokinin and brassinolide as additives into coconut husk matrix; the concentration of the putrescine is 3-7 mmol.L-1The concentration of the cytokinin is 1 x 10-2-3×10-2mg·L-1The concentration of the brassinolide is 8 multiplied by 10-4-1.2×10-3mg·L-1
2. The novel functional matrix according to claim 1, wherein the concentration of putrescine is 5 mmol-L-1The concentration of the cytokinin is 2 x 10-2mg·L-1The concentration of the brassinolide is 10-3mg·L-1
3. A method for preparing a novel functional matrix according to any one of claims 1 to 2, wherein putrescine, cytokinin and brassinolide are mixed, and the uniformly mixed putrescine, cytokinin and brassinolide are added to a coconut coir matrix.
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EP0281374B1 (en) * 1987-03-06 1994-06-01 Mitsui Petrochemical Industries, Ltd. Method of multiplicating plant seedlings
CN101773060B (en) * 2009-01-08 2011-10-19 中国科学院植物研究所 Method for accelerating cucumber growth and special culture medium thereof
CA2976783A1 (en) * 2015-02-19 2016-08-25 Christopher SCANNELL Use of bentonite for improving plant growth-related traits
CN105175149B (en) * 2015-10-12 2019-01-08 春舞枝花卉有限公司 A kind of plants and flowers cultivation matrix and the preparation method and application thereof
CN106258904A (en) * 2016-09-13 2017-01-04 覃淑兰 A kind of Herba Dendrobii Rooting and hardening-off culture base and compound method thereof
CN107673800A (en) * 2017-10-20 2018-02-09 成都先先先生物科技有限公司 A kind of soilless culture nutrient fluid for improving Kidney bean yield and preparation method thereof
CN108059541A (en) * 2018-01-12 2018-05-22 厦门市银农种苗有限公司 A kind of seedling medium and preparation method thereof
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