CN109825427A - A kind of full-automatic cell activity analysis system - Google Patents
A kind of full-automatic cell activity analysis system Download PDFInfo
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- CN109825427A CN109825427A CN201910136775.4A CN201910136775A CN109825427A CN 109825427 A CN109825427 A CN 109825427A CN 201910136775 A CN201910136775 A CN 201910136775A CN 109825427 A CN109825427 A CN 109825427A
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- activity analysis
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- 238000004458 analytical method Methods 0.000 title claims abstract description 15
- 230000000694 effects Effects 0.000 title claims abstract description 14
- 238000003384 imaging method Methods 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000004083 survival effect Effects 0.000 claims abstract description 9
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 claims abstract description 8
- 238000003780 insertion Methods 0.000 claims abstract description 7
- 230000037431 insertion Effects 0.000 claims abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 6
- 230000000007 visual effect Effects 0.000 claims abstract description 6
- 239000001045 blue dye Substances 0.000 claims abstract description 4
- 239000005357 flat glass Substances 0.000 claims abstract description 4
- 238000013500 data storage Methods 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000000975 dye Substances 0.000 abstract description 3
- 230000003833 cell viability Effects 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 3
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000013473 artificial intelligence Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Abstract
The invention discloses a kind of full-automatic cell activity analysis systems, the following steps are included: adding Trypan Blue dye on Cell sheet glass, slide is inserted into, trigger whole system, after imaging, cell concentration is calculated by algorithm, survival rate, slide extractor, imaging process is completed, slide insertion system device, system and device includes panel display screen, operate keyboard, supporting leg, slot, cabinet, control mainboard, fixed link, CCD imager, microscope tube, support rod, fixed block, non-slip mat, support plate, connecting rod, sliding slot, sliding block, support frame and tooth band, the invention, by the dyestuff that the cell on slide is added to trypan blue, control CCD imaging, 5 visuals field are clapped altogether, data are transferred in computer, cell concentration is calculated by algorithm, survival rate etc., intelligence imaging, machine processing is fast, It is not easy to make mistakes, automatically, as long as slide is inserted into, immediately arrive at as a result, it is possible to directly, it is convenient to data acquisition and cell viability analyze.
Description
Technical field
The present invention relates to artificial intelligence equipment technical field, specially a kind of full-automatic cell activity analysis system.
Background technique
In Bioexperiment, cell experiment is indispensable a part, and cell experiment necessarily involves cell count, mesh
Preceding cell count work is more, and cumbersome, a cell count averagely needs to spend 20 minutes, time-consuming by artificial counting, wastes people
Power, and exist and think operating error.There are cell counter similar products on the market, but be not full-automation, this causes right
When cell count concentration calculation and survival rate calculate, generates and centainly think operating error.
Summary of the invention
It is above-mentioned to solve the purpose of the present invention is to provide a kind of full-automatic cell activity analysis system and its manufacturing method
The problem of being proposed in background technique.
In order to solve the above technical problem, the present invention provides following technical solutions: its technical characterstic has；
1) by automation and intelligent algorithm, cell count efficiency is greatly improved；
2) full-automatic, it substantially reduces and thinks operating error；
3) analysis of accounts is detected in five visuals field, further increases accuracy rate and stability；
4) integrated technology improves system stability, service life, information security.
Full-automatic cell activity analysis system, includes the following steps:
1) Trypan Blue dye is added on Cell sheet glass；
2) slide is inserted into, triggers whole system；
3) after imaging, cell concentration, survival rate are calculated by algorithm；
4) slide extractor, imaging process are completed.
According to the above technical scheme, the slide insertion system device, system and device include panel display screen, operation key
Disk, supporting leg, slot, cabinet, control mainboard, fixed link, CCD imager, microscope tube, support rod, fixed block, non-slip mat,
Support plate, connecting rod, sliding slot, sliding block, support frame, tooth band, fluted disc and stepper motor, the panel display screen installation by adhering exist
On front side of the outer wall of cabinet, the operation keyboard is mounted on front side of outer wall, and is located at the lower section of panel display screen, the bottom of the cabinet
Side quadrangle is equipped with supporting leg, and the side of the cabinet offers slot, and the control mainboard is mounted on the inner wall side of cabinet,
The top of the fixed link is fixed on the inner wall top side of cabinet, and the CCD imager is fixed on the bottom end of fixed link, the support
One end of bar is fixed on the inner wall side of cabinet, and the microscope tube is fixed on the other end of support rod, and microscope tube vertically
It is fixed on the underface of CCD imager, support frame as described above is fixed on the inner wall top side of cabinet, and the sliding slot is provided with support frame
Inside top side, the sliding block is mounted on the inside of sliding slot, and the connecting rod is fixed on the top side of sliding block, the support plate peace vertically
Mounted in the top of connecting rod, the non-slip mat is pasted in the top side of support plate, and the tooth band is arranged in the bottom surface side of support plate,
The fixed block is fixed on the side of support frame, and the stepper motor is fixed on the top side of fixed block, and the fluted disc is mounted on step
Into the output end of motor.
According to the above technical scheme, the slot corresponds to support plate and opens up, and the tooth band and fluted disc are engaging structure.
According to the above technical scheme, the microscope tube is located at the top side of support plate, and the CCD imager is arranged micro-
The top of lens barrel.
According to the above technical scheme, the control mainboard include image capture module, data storage module, computing module and
Information communication module, described image acquisition module and computing module are connect with information communication module by signal wire, the data
Storage module is connect with image capture module and computing module by signal.
According to the above technical scheme, it in the step 3, before and after slide insertion, needs to carry out disinfection to whole system,
Keep gnotobasis.
According to the above technical scheme, six slots are arranged in the slide, when by field of microscope, are controlled by control mainboard
CCD imaging, claps altogether 5 visuals field, and data are transferred in the data storage module of control mainboard.
Compared with prior art, the beneficial effects obtained by the present invention are as follows being: the invention, by the way that the cell on slide is added
The dyestuff of trypan blue, control CCD imaging, claps altogether 5 visuals field, and data are transferred in computer, calculate cell by algorithm
Concentration, survival rate etc., intelligence are imaged, and machine processing is fast, not easy to make mistakes, automatically, as long as slide is inserted into, immediately arrive at knot
Fruit, can be direct, convenient to analyze data acquisition and cell viability.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is overall structure diagram of the invention；
Fig. 2 is whole interior structural schematic diagram of the invention；
Fig. 3 is the a-quadrant structural schematic diagram in Fig. 2 of the invention；
Fig. 4 is operational flowchart of the invention；
In figure: 1, panel display screen；2, keyboard is operated；3, supporting leg；4, slot；5, cabinet；6, control mainboard；7, fixed link；
8, CCD imager；9, microscope tube；10, support rod；11, fixed block；12, non-slip mat；13, support plate；14, connecting rod；15,
Sliding slot；16, sliding block；17, support frame；18, tooth band；19, fluted disc；20, stepper motor.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-4 is please referred to, the present invention provides a kind of technical solution: a kind of full-automatic cell activity analysis system, including such as
Lower step:
1) Trypan Blue dye is added on Cell sheet glass；
2) slide is inserted into, triggers whole system；
3) after imaging, cell concentration, survival rate are calculated by algorithm；
4) slide extractor, imaging process are completed.
According to the above technical scheme, slide insertion system device, system and device include panel display screen 1, operation keyboard 2,
Supporting leg 3, slot 4, cabinet 5, control mainboard 6, fixed link 7, CCD imager 8, microscope tube 9, support rod 10, fixed block 11,
Non-slip mat 12, support plate 13, connecting rod 14, sliding slot 15, sliding block 16, support frame 17, tooth band 18, fluted disc 19 and stepper motor 20,
On front side of the outer wall of cabinet 5, operation keyboard 2 is mounted on front side of outer wall 1 installation by adhering of panel display screen, and is located at FPD
The lower section of screen 1, the bottom side quadrangle of cabinet 5 are equipped with supporting leg 3, and the side of cabinet 5 offers slot 4, and control mainboard 6 is mounted on
The inner wall side of cabinet 5, the top of fixed link 7 are fixed on the inner wall top side of cabinet 5, and CCD imager 8 is fixed on fixed link 7
Bottom end, one end of support rod 10 are fixed on the inner wall side of cabinet 5, and microscope tube 9 is fixed on the other end of support rod 10 vertically,
And microscope tube 9 is fixed on the underface of CCD imager 8, support frame 17 is fixed on the inner wall top side of cabinet 5, and sliding slot 15 opens up
Inside the top side of support frame 17, sliding block 16 is mounted on the inside of sliding slot 15, and connecting rod 14 is fixed on the top side of sliding block 16 vertically,
Support plate 13 is mounted on the top of connecting rod 14, and non-slip mat 12 is pasted in the top side of support plate 13, and tooth band 18 is arranged in support plate
13 bottom surface side, fixed block 11 are fixed on the side of support frame 17, and stepper motor 20 is fixed on the top side of fixed block 11, fluted disc
19 are mounted on the output end of stepper motor 20, and the corresponding support plate 13 of slot 4 opens up, and tooth band 18 and fluted disc 19 are engaging structure,
Microscope tube 9 is located at the top side of support plate 13, and the top of microscope tube 9 is arranged in CCD imager 8.
According to the above technical scheme, control mainboard 6 includes image capture module, data storage module, computing module and letter
Cease communication module, image capture module and computing module connect with information communication module by signal wire, data storage module and
Image capture module is connected with computing module by signal.
According to the above technical scheme, it in step 3, before and after slide insertion, needs to carry out disinfection to whole system, keep
Gnotobasis.
According to the above technical scheme, six slots are arranged in slide, when by field of microscope, control CCD by control mainboard
Imaging, claps altogether 5 visuals field, and data are transferred in the data storage module of control mainboard.
Based on above-mentioned, it is an advantage of the current invention that it is of the invention, slide is inserted into inside cabinet 5 by slot 4, and is placed
In the top side of support plate 13, at this moment 20 operation of stepper motor, band active toothed disk 19 rotates, and is cooperated by tooth band 18, while sliding block 16
15 is mobile along the chute, realizes that support plate 13 is mobile in microscope tube 9, the slot inside slide is corresponded to the underface of microscope tube 9,
Image is acquired by CCD imager 8, inside image transmitting to control mainboard 6, is calculated by computing module, to cytometer
Particle density calculates, and in imaging picture, finds out the circle for meeting cell shape, is determined as cell, what bonding occurred in cell, can be with
The number of agglomeration cell is calculated according to single area according to the cell gross area of bonding agglomeration, when calculating survival rate,
Using Trypan Blue, after trypan blue is added, living cells is not colored, and dead cell is by dye blue.In imaging picture, it is not colored
Living cells, dead cell that is white, and being colored among cell, cell is in black, and algorithm on this basis, determines that cell is dead
Living, to calculate survival rate, last stepper motor 20 is reversed, and drives support plate 13 to retreat, slide extractor was imaged
Journey is completed.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (7)
1. a kind of full-automatic cell activity analysis system, which comprises the steps of:
1) Trypan Blue dye is added on Cell sheet glass；
2) slide is inserted into, triggers whole system；
3) after imaging, cell concentration, survival rate are calculated by algorithm；
4) slide extractor, imaging process are completed.
2. a kind of full-automatic cell activity analysis system according to claim 1, it is characterised in that: slide insertion system
Bulk cargo is set, and system and device includes panel display screen (1), operation keyboard (2), supporting leg (3), slot (4), cabinet (5), control master
Plate (6), fixed link (7), CCD imager (8), microscope tube (9), support rod (10), fixed block (11), non-slip mat (12), branch
Fagging (13), connecting rod (14), sliding slot (15), sliding block (16), support frame (17), tooth band (18), fluted disc (19) and stepper motor
(20), for panel display screen (1) installation by adhering on front side of the outer wall of cabinet (5), the operation keyboard (2) is mounted on outer wall
Front side, and it is located at the lower section of panel display screen (1), the bottom side quadrangle of the cabinet (5) is equipped with supporting leg (3), the cabinet
(5) side offers slot (4), and the control mainboard (6) is mounted on the inner wall side of cabinet (5), the fixed link (7)
Top is fixed on the inner wall top side of cabinet (5), and the CCD imager (8) is fixed on the bottom end of fixed link (7), the support rod
(10) one end is fixed on the inner wall side of cabinet (5), and the microscope tube (9) is fixed on the another of support rod (10) vertically
End, and microscope tube (9) is fixed on the underface of CCD imager (8), support frame as described above (17) is fixed on the inner wall of cabinet (5)
Top side, the sliding slot (15) are provided with inside the top side of support frame (17), and the sliding block (16) is mounted on the inside of sliding slot (15),
The connecting rod (14) is fixed on the top side of sliding block (16) vertically, and the support plate (13) is mounted on the top of connecting rod (14),
The non-slip mat (12) is pasted in the top side of support plate (13), and the tooth band (18) is arranged in the bottom surface side of support plate (13),
The fixed block (11) is fixed on the side of support frame (17), and the stepper motor (20) is fixed on the top side of fixed block (11),
The fluted disc (19) is mounted on the output end of stepper motor (20).
3. a kind of full-automatic cell activity analysis system according to claim 2, it is characterised in that: the slot (4) is right
Support plate (13) is answered to open up, the tooth band (18) and fluted disc (19) are engaging structure.
4. a kind of full-automatic cell activity analysis system according to claim 2, it is characterised in that: the microscope tube
(9) it is located at the top side of support plate (13), top of CCD imager (8) setting in microscope tube (9).
5. a kind of full-automatic cell activity analysis system according to claim 2, it is characterised in that: the control mainboard
It (6) include image capture module, data storage module, computing module and information communication module, described image acquisition module and meter
It calculates module to connect with information communication module by signal wire, the data storage module and image capture module and computing module lead to
Cross signal connection.
6. a kind of full-automatic cell activity analysis system according to claim 1, it is characterised in that: in the step 3,
It before and after slide insertion, needs to carry out disinfection to whole system, keeps gnotobasis.
7. a kind of full-automatic cell activity analysis system according to claim 1, it is characterised in that: the slide setting six
A slot by control mainboard control CCD imaging, claps altogether 5 visuals field, data are transferred to control master when by field of microscope
In the data storage module of plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910136775.4A CN109825427A (en) | 2019-02-25 | 2019-02-25 | A kind of full-automatic cell activity analysis system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910136775.4A CN109825427A (en) | 2019-02-25 | 2019-02-25 | A kind of full-automatic cell activity analysis system |
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| Publication Number | Publication Date |
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| CN109825427A true CN109825427A (en) | 2019-05-31 |
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| CN201910136775.4A Pending CN109825427A (en) | 2019-02-25 | 2019-02-25 | A kind of full-automatic cell activity analysis system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113188979A (en) * | 2021-04-28 | 2021-07-30 | 河南省人民医院 | Full-automatic biochemical analyzer for tumor cell aging detection |
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|---|---|---|---|---|
| US20100246927A1 (en) * | 2009-03-25 | 2010-09-30 | Arbuckle John D | Darkfield imaging system and methods for automated screening of cells |
| US20110211058A1 (en) * | 2009-08-31 | 2011-09-01 | Bio-Rad Laboratories, Inc. | Compact automated cell counter |
| WO2012166952A1 (en) * | 2011-05-31 | 2012-12-06 | Bio-Rad Laboratories, Inc. | Cell counting systems and methods |
| CN103146800A (en) * | 2013-03-27 | 2013-06-12 | 朱耀辉 | Full-automatic cell non-staining image recognizing and counting method |
| JP2014082957A (en) * | 2012-10-19 | 2014-05-12 | Olympus Corp | Cell counting apparatus and cell counting program |
-
2019
- 2019-02-25 CN CN201910136775.4A patent/CN109825427A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100246927A1 (en) * | 2009-03-25 | 2010-09-30 | Arbuckle John D | Darkfield imaging system and methods for automated screening of cells |
| US20110211058A1 (en) * | 2009-08-31 | 2011-09-01 | Bio-Rad Laboratories, Inc. | Compact automated cell counter |
| WO2012166952A1 (en) * | 2011-05-31 | 2012-12-06 | Bio-Rad Laboratories, Inc. | Cell counting systems and methods |
| JP2014082957A (en) * | 2012-10-19 | 2014-05-12 | Olympus Corp | Cell counting apparatus and cell counting program |
| CN103146800A (en) * | 2013-03-27 | 2013-06-12 | 朱耀辉 | Full-automatic cell non-staining image recognizing and counting method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113188979A (en) * | 2021-04-28 | 2021-07-30 | 河南省人民医院 | Full-automatic biochemical analyzer for tumor cell aging detection |
| CN113188979B (en) * | 2021-04-28 | 2022-03-18 | 河南省人民医院 | Full-automatic biochemical analyzer for tumor cell aging detection |
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