CN109799181A - A kind of blood count device and method based on load quantity - Google Patents
A kind of blood count device and method based on load quantity Download PDFInfo
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- CN109799181A CN109799181A CN201910085410.3A CN201910085410A CN109799181A CN 109799181 A CN109799181 A CN 109799181A CN 201910085410 A CN201910085410 A CN 201910085410A CN 109799181 A CN109799181 A CN 109799181A
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- control circuit
- timing
- start unit
- haemocyte
- load quantity
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Abstract
The invention discloses a kind of hemocytometric procedures based on load quantity, including electrical impedance cell detection device, it further include control circuit and with the sequentially connected solenoid valve of conduit, micro flowmeter, timing start unit and waste drains pump, the liquid feeding end of the solenoid valve is connected in the reaction rear chamber with conduit, and the electrical impedance cell detection device, solenoid valve, micro flowmeter, timing start unit and waste drains pump are connect with the control circuit.Blood count device and method based on load quantity of the invention, not only the cell load quantity for participating in counting can be fixed on a biggish value, simultaneously, the volume for flowing through the haemocyte dilution of micropore can be calculated multiplied by the measured value of flowmeter micro in gate time by gate time, it eliminates and determines volume devices, it can simplify liquid channel system, reduce structure space, the haemocyte less for content in blood, also biggish cell numerical value can be measured, the measurement accuracy of instrument is improved.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of hemocytometer counting apparatus based on load quantity and
Method.
Background technique
In recent years, being widely used with scientific and technological progress and computer, some self-reacting devices are largely introduced in blood testing,
Blood analyser is that hospital clinical examines one of widely applied instrument.It makes the processing of blood count simpler, fast
It is fast, accurate, the working strength of medical staff is effectively alleviated, is improved work efficiency.
Currently, most of blood analysers are all made of theory of electrical impedance (Coulter principle) to detect haemocyte.It is using
Under the premise of theory of electrical impedance carries out blood count, an important parameter for measuring blood analyser performance quality is exactly instrument
To the CV value of haemocyte measurement, CV value is lower, illustrates that the otherness of the apparatus measures is smaller, the precision of measurement is also higher.
The CV coefficient of variation (coefficient of variation), also known as coefficient of dispersion (coefficient of
Dispersion) or relative deviation (rsd), it is the ratio between standard deviation and average value, is indicated with percentage, calculation formula are as follows: CV
=SD/E × 100% (SD is standard deviation, and E is variance).By the calculation formula of CV it is recognised that improve apparatus measures essence
Degree reduces CV value, it would be desirable to reduce SD, calculation formula:By SD calculation formula I
It is recognised that the value that reduce SD will improve the count number of haemocyte.
Currently, generalling use the counting for determining volume using the blood analyser that theory of electrical impedance (Coulter principle) detects
Method will count the cell number in certain volume with blood analyser after hemodilution certain multiple, and be converted into every liter of blood
Cell number in liquid.Since the method for counting is simple, speed is fast, blood analyser is met to a certain extent to blood count
Needs, but there is also some shortcomingss: 1. when hemodilution ratio is too low, when hematocrite concentration is higher, not only wasting
Blood sample, while blood count frequency is excessively high, causes certain difficulty to circuit design and signal sampling.Excessively high hematocrite concentration
It is easier to cause the blocking of resistance anti-detection devices micropore.2. specified when hematocrite concentration is lower when hemodilution ratio is excessively high
The cell quantity measured in volume will be lower, has a huge impact to instrument CV value, influences the judgement of doctor.Meanwhile it is right
The less cell of content in blood needs bigger volume devices of determining, therefore this method is dilute to blood if improving detection accuracy
Release the narrow scope of application of ratio.3. since cell number magnitude is larger, under specified volume, since the odjective causes such as vibration are drawn
The variation that the micro-scale volume detection error risen all can cause cell detection numerical value very big, the detection accuracy of lowering apparatus.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of blood count device and method based on load quantity, i.e.,
Haemocyte dilution will be formed after hemodilution certain multiple, electricity consumption impedance cell detection device counts the cell of load quantity,
The body for flowing through the haemocyte dilution of micropore is calculated multiplied by the measured value of flowmeter micro in gate time by gate time
Product, and the blood cell count being converted into every liter of blood.
The present invention solves above-mentioned technical problem by following technological means:
A kind of hemocytometer counting apparatus based on load quantity, including electrical impedance cell detection device, the inspection of resistance anti-cell
Surveying device includes reaction cup and reaction rear chamber, and the reaction cup is connected with reaction rear chamber by a micropore, the reaction
Interior electrode is installed in cup, external electrode is installed in the reaction rear chamber, the inner and outer electrodes connect on constant-current source,
It further include control circuit and with the sequentially connected solenoid valve of conduit, micro flowmeter, timing start unit and waste drains pump, the electricity
The liquid feeding end of magnet valve is connected in the reaction rear chamber with conduit, the electrical impedance cell detection device, solenoid valve, micro flow
Meter, timing start unit and waste drains pump are connect with the control circuit.
Further, the timing start unit is to detect the detection optocoupler for having achroacyte dilution in conduit, when described
When haemocyte diluent stream is through timing start unit, the timing start unit sends timing enabling signal, institute to control circuit
It states control circuit and starts timing.
Further, waste collection bottle is connected with conduit on the outlet end of the waste drains pump.
The present invention provides a kind of hemocytometric procedures based on load quantity, comprising:
The hemocytometer counting apparatus based on load quantity is provided；
Haemocyte dilution will be obtained after hemodilution certain multiple, into the reaction cup of electrical impedance cell detection device
A certain amount of haemocyte dilution is added；
Waste drains pump is opened, reacts the haemocyte dilution in cup under the extraction of waste drains pump, is successively led to from reaction cup
Micropore is crossed, rear chamber, solenoid valve, micro flowmeter, timing start unit and waste drains pump is reacted, is then discharged out waste liquid；
Trace stream flowmeter measurement flows through the flow of the haemocyte dilution of micro flowmeter, obtains flow value Q；
When timing start unit detects haemocyte diluent stream through timing start unit, the timing start unit to
Control circuit sends timing enabling signal, and the control circuit starts timing, and the electrical impedance cell detection device starts to carry out
Blood count；
Control circuit receives the count value of blood count from electrical impedance cell detection device, when the count value reaches
To after rating number, the control circuit stops timing, obtains timing time t；
Control circuit calculates the volume V for flowing through the haemocyte dilution of micropore according to formula V=Q*t, and converses every
Rise the cell number in blood.
Further, the control circuit receives the count value of blood count from electrical impedance cell detection device, works as institute
It states after count value reaches rating number, further includes that the solenoid valve is closed, and the waste drains pump is in emptying solenoid valve to waste drains pump
It is closed after haemocyte dilution in this section of pipeline.
Further, after the waste liquid pump startup, the timing start unit is not sent to control circuit within the set time
When timing enabling signal, the control circuit sends warning signal.
Beneficial effects of the present invention:
Blood count device and method based on load quantity of the invention can will not only participate in the cell volume counted
Fixed number amount is fixed on a biggish value, meanwhile, it can be by gate time multiplied by the measurement of flowmeter micro in gate time
Value calculates the volume for flowing through the haemocyte dilution of micropore, eliminates and determines volume devices, can simplify liquid channel system, reduces knot
Conformational space saves medical space.Meanwhile the haemocyte (such as leucocyte, blood platelet) less for content in blood, also can
Biggish cell numerical value is measured, the measurement accuracy of instrument is improved, also, measures volume not by objective factors such as extraneous vibrations
It influences, the reliability for improving instrument has very high meaning.In addition, the hemodilution ratio of the present invention couple and insensitive, it can
To be applicable in broader hemodilution ratio.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of the hemocytometer counting apparatus based on load quantity of the present invention；
Fig. 2 is a kind of flow chart of the hemocytometric procedure based on load quantity of the present invention；
Wherein, cup 1, reaction rear chamber 2, micropore 3, interior electrode 4, external electrode 5, solenoid valve 6, micro flowmeter 7, meter are reacted
When start unit 8, waste drains pump 9, waste collection bottle 10.
Specific embodiment
Below with reference to the drawings and specific embodiments, the present invention is described in detail:
As shown in Figure 1, a kind of hemocytometer counting apparatus based on load quantity of the invention, including electrical impedance cell detection
Device, electrical impedance cell detection device include reaction cup 1 and reaction rear chamber 2, and the reaction cup and reaction rear chamber pass through one
Micropore 3 is connected, and interior electrode 4 is equipped in the reaction cup 1, and external electrode 5 is equipped in the reaction rear chamber 2, described interior
Electrode 4 and external electrode 5 connect on constant-current source, further include control circuit and with the sequentially connected solenoid valve 6 of conduit, micro flowmeter
7, the liquid feeding end of timing start unit 8 and waste drains pump 9, the solenoid valve 6 is connected in the reaction rear chamber 2 with conduit, described
Electrical impedance cell detection device, solenoid valve 6, micro flowmeter 7, timing start unit 8 and waste drains pump 9 with the control circuit
Connection.
Specifically, the timing start unit 8 is to detect the detection optocoupler for having achroacyte dilution in conduit, when described
When haemocyte diluent stream is through timing start unit 8, the timing start unit 8 sends timing enabling signal to control circuit,
The control circuit starts timing.
Further, waste collection bottle 10 is connected with conduit on the outlet end of the waste drains pump 9.Waste collection bottle 10 is used for
Collection flows through the haemocyte dilution after waste drains pump 9.
The present invention provides a kind of hemocytometric procedures based on load quantity, comprising:
The hemocytometer counting apparatus based on load quantity is provided；
Haemocyte dilution will be obtained after hemodilution certain multiple, to the reaction cup 1 of electrical impedance cell detection device
It is interior that a certain amount of haemocyte dilution is added；
Open waste drains pump 9, react cup 1 in haemocyte dilution under the extraction of waste drains pump 9, from reaction cup 1 according to
It is secondary by micropore 3, react rear chamber 2, solenoid valve 6, micro flowmeter 7, timing start unit 8 and waste drains pump 9, be then discharged out useless
Liquid；
Micro flowmeter 7 measures the flow for flowing through the haemocyte dilution of micro flowmeter 7, obtains flow value Q；
When timing start unit 8 detects haemocyte diluent stream through timing start unit 8, the timing start unit
8 send timing enabling signal to control circuit, and the control circuit starts timing, the electrical impedance cell detection device start into
Promoting circulation of blood cell count；
Control circuit receives the count value of blood count from electrical impedance cell detection device, when the count value reaches
To after rating number, the control circuit stops timing, obtains timing time t；
Control circuit is according to formula V=Q*t, after calculating the volume V for flowing through the haemocyte dilution of micropore 3, according to number
Value and unit conversion relationship, can converse the cell number in every liter of blood.
Further, the control circuit receives the count value of blood count from electrical impedance cell detection device, works as institute
It states after count value reaches rating number, further includes that the solenoid valve is closed, and the waste drains pump is in emptying solenoid valve to waste drains pump
It is closed after haemocyte dilution in this section of pipeline.Emptying solenoid valve is to the liquid in this section of pipeline of waste drains pump, for the meter of next time
Number is prepared.
Further, after the waste liquid pump startup, the timing start unit is not sent to control circuit within the set time
When timing enabling signal, the control circuit sends warning signal.After the waste liquid pump startup, if timing starting is single
Timing start unit is not to control circuit after member does not send timing enabling signal, such as 10s to control circuit within the set time
Send timing enabling signal, then illustrate haemocyte dilution fail reach timing start unit at, then may be fluid path be plugged or
It is other problems occur, control circuit sends warning signal and reminds operator's maintenance overhaul in time, debugging.
In conclusion the blood count device and method of the invention based on load quantity, can will not only participate in counting
Several cell load quantities is fixed on a biggish value, meanwhile, it can be by gate time multiplied by trace stream in gate time
The measured value of meter calculates the volume for flowing through the haemocyte dilution of micropore, eliminates and determines volume devices, can simplify fluid path
System reduces structure space, saves medical space.Meanwhile (such as leucocyte, blood is small for the haemocyte less for content in blood
Plate etc.), biggish cell numerical value can be also measured, the measurement accuracy of instrument is improved, also, measures volume not by extraneous vibration
Etc. objective factors influence, for improve instrument reliability have very high meaning.In addition, the hemodilution ratio of the present invention couple
And it is insensitive, broader hemodilution ratio can be applicable in.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferred embodiment to this hair
It is bright to be described in detail, those skilled in the art should understand that, it can modify to technical solution of the present invention
Or equivalent replacement should all cover without departing from the objective and range of technical solution of the present invention in claim of the invention
In range.Technology not described in detail in the present invention, shape, construction portion are well-known technique.
Claims (6)
1. a kind of hemocytometer counting apparatus based on load quantity, including electrical impedance cell detection device, electrical impedance cell detection
Device includes reaction cup and reaction rear chamber, and the reaction cup is connected with reaction rear chamber by a micropore, before the reaction
Indoor location has interior electrode, is equipped with external electrode in the reaction rear chamber, and the inner and outer electrodes connect on constant-current source,
It is characterized in that: further including control circuit and with the sequentially connected solenoid valve of conduit, micro flowmeter, timing start unit and waste liquid
Pump, the liquid feeding end of the solenoid valve are connected in the reaction rear chamber with conduit, the electrical impedance cell detection device, electromagnetism
Valve, micro flowmeter, timing start unit and waste drains pump are connect with the control circuit.
2. a kind of hemocytometer counting apparatus based on load quantity according to claim 1, which is characterized in that the timing
Start unit is to detect the detection optocoupler for having achroacyte dilution in conduit, when the haemocyte diluent stream starts through timing
When unit, the timing start unit sends timing enabling signal to control circuit, and the control circuit starts timing.
3. a kind of hemocytometer counting apparatus based on load quantity according to claim 2, which is characterized in that the waste liquid
Waste collection bottle is connected with conduit on the outlet end of pump.
4. a kind of hemocytometric procedure based on load quantity characterized by comprising
Hemocytometer counting apparatus as claimed in any one of claims 1-3 based on load quantity is provided；
Haemocyte dilution will be obtained after hemodilution certain multiple, is added one to the reaction cup of electrical impedance cell detection device
Quantitative haemocyte dilution；
Waste drains pump is opened, reacts the haemocyte dilution in cup under the extraction of waste drains pump, is passed sequentially through from reaction cup micro-
Hole reacts rear chamber, solenoid valve, micro flowmeter, timing start unit and waste drains pump, is then discharged out waste liquid；
Trace stream flowmeter measurement flows through the flow of the haemocyte dilution of micro flowmeter, obtains flow value Q；
When timing start unit detects haemocyte diluent stream through timing start unit, the timing start unit is to control
Circuit sends timing enabling signal, and the control circuit starts timing, and it is thin that the electrical impedance cell detection device starts progress blood
Born of the same parents count；
Control circuit receives the count value of blood count from electrical impedance cell detection device, when the count value reaches volume
After fixed number value, the control circuit stops timing, obtains timing time t；
Control circuit calculates the volume V for flowing through the haemocyte dilution of micropore according to formula V=Q*t, and converses every liter of blood
Cell number in liquid.
5. a kind of hemocytometric procedure based on load quantity according to claim 4, which is characterized in that the control
Circuit receives the count value of blood count from electrical impedance cell detection device, when the count value reaches rating number
It afterwards, further include that the solenoid valve is closed, the waste drains pump is in emptying solenoid valve to the haemocyte dilution in this section of pipeline of waste drains pump
It is closed after liquid.
6. a kind of hemocytometric procedure based on load quantity according to claim 5, which is characterized in that when described useless
After liquid pump starting, when the timing start unit to control circuit does not send timing enabling signal within the set time, the control
Circuit sends warning signal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910085410.3A CN109799181A (en) | 2019-01-29 | 2019-01-29 | A kind of blood count device and method based on load quantity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910085410.3A CN109799181A (en) | 2019-01-29 | 2019-01-29 | A kind of blood count device and method based on load quantity |
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3529792A1 (en) * | 1984-08-24 | 1986-02-27 | Univ Rene Descartes | METHOD AND DEVICE FOR DETERMINING THE DEFORMABILITY OF RED BLOOD BODIES |
| CN101460827A (en) * | 2003-08-13 | 2009-06-17 | 卢米尼克斯股份有限公司 | Methods for controlling one or more parameters of a flow cytometer type measurement system |
| JP2007017302A (en) * | 2005-07-07 | 2007-01-25 | Horiba Ltd | Measuring instrument of number of particles |
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| CN101581596A (en) * | 2009-06-05 | 2009-11-18 | 浙江省计量科学研究院 | Flow detecting method based on double-time measurement technology and apparatus thereof |
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Application publication date: 20190524 |