CN109796273A - A kind of method of microbial flora processing feces of livestock and poultry - Google Patents
A kind of method of microbial flora processing feces of livestock and poultry Download PDFInfo
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- CN109796273A CN109796273A CN201910016762.3A CN201910016762A CN109796273A CN 109796273 A CN109796273 A CN 109796273A CN 201910016762 A CN201910016762 A CN 201910016762A CN 109796273 A CN109796273 A CN 109796273A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to poultry excrement and urine disposal field, a kind of method for being specifically related to microbial flora processing feces of livestock and poultry, this method is to be mixed to get mixed material B with feces of livestock and poultry again after first mixing tobacco leftovers and molasses；Then microbial flora is added in mixed material B to be uniformly mixed, obtains mixed material C, after the moisture content for adjusting mixed material C, be placed in fermentation bed and ferment, finally obtain tunning, tunning can be used as farmyard manure directly using or for making organic fertilizer.The present invention carries out fermentation process to feces of livestock and poultry using microbial flora, it is good in the deodorization of entire fermentation process feces of livestock and poultry, fly eradication maggot effect, it is energy conservation and environmental protection, free from environmental pollution, and this method processing step is simple, it is easily operated, the processing cost in aquaculture industry to feces of livestock and poultry is significantly reduced, the surcharge and utilization rate of feces of livestock and poultry are substantially increased.
Description
Technical field
The present invention relates to poultry excrement and urine disposal fields, are specifically related to a kind of side of microbial flora processing feces of livestock and poultry
Method.
Background technique
Feces of livestock and poultry is by indigested feed nutrient, interior metabolism product, gastrointestinal mucosal, secretion, the micro- life of enteron aisle
Object and its decomposition product etc. collectively constitute.Feces of livestock and poultry is both the environmental hazard substance of reality a kind of and a kind of available
Living resources, to its using it is improper will be serious pollution sources, if realize harmless treatment, can largely play livestock and poultry
The effect of the resources such as excrement biomass energy, waste nutrition, realizes the recycling of waste resource.The comprehensive benefit of feces of livestock and poultry at present
Mode is used mainly include the following types: 1) as Fertilizer application: feces of livestock and poultry is a kind of good organic fertilizer resource of agricultural production, will
Waste utilizes after carrying out compost treatment, is that China uses wide form at present.However in composting process, due to liquid dung
Leak and lower infiltration and cause the pollution of environment.2) it is used as feedstuff: containing many nutriments in feces of livestock and poultry, especially disappear
Change the shorter birds in road, the nutrient content in excrement is higher.But since the palatability of excrement is poor, contains pathogenic microorganism and can
The problems such as digestibility is low, and limit its use.3) it utilizes biogas project: containing a large amount of organic matter in feces of livestock and poultry,
Under high temperature (35 DEG C~55 DEG C) anaerobic condition through microbial degradation be biogas and carbon dioxide.By biogas fermentation, excrement can be killed
Pathogenic microorganism in water, however there is also some potential safety problemss.4) feces of livestock and poultry is handled using deodorant: to mitigate livestock and poultry
The pollution of excreta and its smell, can add all kinds of deodorant in feed or padding, and the method processing cost is higher.5) aerobic
Compost treatment, aerobic compost are exactly under manual control, under certain humidity, temperature, C/N ratio and ventilation condition, using certainly
The fermentation of the microorganisms such as the widely distributed bacterium in right boundary, actinomyces, fungi, artificially promotes biodegradable organic matter
To the microbiological process of stable humus biochemical conversion.Aerobic compost processing be realize feces of livestock and poultry is innoxious, minimizing,
Recycling, the maximally efficient method of one kind of industrialization, because it is low with operating cost, treating capacity is big, without secondary pollution etc. excellent
Point, oneself becomes a research hotspot of countries in the world resource, environment protection field in recent years.But no matter select anaerobic fermentation or aerobic hair
Ferment, it is general time-consuming all longer, short then a couple of days, long then to take 3-4 months ability decomposed, and the foul smell given out not only polluted environment but also
Nutrient is caused to be largely lost.Feces of livestock and poultry is handled using compost method, the final product foul smell of processing is less, and relatively dry, holds
It is easy to package, spread fertilizer over the fields.The disadvantage is that there is the loss of NH3 in treatment process, foul smell cannot be fully controlled, the place that compost needs is big, place
It is long to manage the time.
In recent years, there are also documents to disclose the processing method of feces of livestock and poultry, such as: 1, application No. is 01107539.2
Chinese patent disclose the consistent method using feces of livestock and poultry production biological organic fertilizer, the invention first respectively mushroom residue,
Chinese medicine slag smash it through the sieve of 15~40 mesh, then feces of livestock and poultry, mushroom residue, Chinese medicine slag, mixed microorganism strain, mixing
Inorganic fertilizer, adhesive in 25~35: 25~35: 25~35: 2~3: 5~8: 1~2 ratio after mixing, be added it is suitable
Water to material moisture is 20~40%, and merging fermentation vat ferments 5~15 days at normal temperature, in fermentation processes fermentation vat
Temperature carries out aeration appropriate and stirring at 40~70 DEG C, then the material Jing Guo above-mentioned processing taken out from fermentation into
Row prebake, granulation are dried again, which takes full advantage of the leftover bits and pieces in farm and sideline product processing industry, resourceful, system
It makes simply, there is significant economic benefit and social benefit.
2, application No. is 201210251613.3 Chinese patents to disclose a kind of decontamination of human excreta recycling treatment side
Method, this method are related to fecal sewage treatment technical field, comprising the following steps: separation of solid and liquid, flocculation dewatering, continuous aerobic heap
Fertilizer gathers chelation treatment and oxygen processing, level-one Aerobic Process for Treatment, second level Aerobic Process for Treatment and comprehensive deodorization, realizes that sewage treatment is harmless
Change discharge, excrement slag resource utilization.
3, application No. is 01101879.8 Chinese patents to disclose a kind of intensive livestock and poultry farm, Animal manure loss processing
The technology invention uses M desiccation, cleanser, completes in field treatment plant.It is greater than 90% feces of livestock and poultry to water content, send to field
Between treatment plant, admix lime exudation excess water, it is aqueous to drop to 75% or so, filter out liquid manure and imposed on nearby after liquid manure pond is decomposed
Field.It in wet the excrement of shape semisolid, then no longer seeps water after admixing M desiccation cleanser, also eliminates stink.It is decomposed to be placed in ventilation
After pond is decomposed, can entrucking fill generation, export trade supply production green food organic fertilizer.Processing or this it is per ton 12~20 yuan, sale price
150~350 yuan/ton, benefit is 1~2 times of existing livestock and poultry farm
4, application No. is 201010233989.2 Chinese patents to disclose a treatment method of poultry manure, and brid guano is placed in by this method
During 500-650 DEG C of high temperature negative pressure is distinguished and admirable, it is stirred for crushing brid guano；In the distinguished and admirable middle holding one of high temperature negative pressure after enabling brid guano crush
After the section time, brid guano from the distinguished and admirable middle separation of high temperature negative pressure and is discharged；Brid guano should make in the time of the distinguished and admirable middle holding of high temperature negative pressure
Brid guano is only heated to 95-105 DEG C by high temperature negative pressure is distinguished and admirable when must discharge；Airflow regulation vacuum magnitude crushing brid guano
In the process, brid guano internal moisture can be enabled to reach boiling point evaporation when brid guano own temperature reaches 70-80 DEG C；So that brid guano
Internal foul smell molecule stirring crush and the distinguished and admirable collective effect of high temperature negative pressure under from brid guano overflow and by pyrolytic.The party
Method has drying efficiency height, good deodorization effect, the spy that the brid guano bacterial content without odor pollution, processing is few but nutritive value is high
Point.
China develops aerobic composting fermentation method, greenhouse fermentation seasoning, anaerobism hair in terms of Animal manure loss improvement
The Treatment process such as ferment facture.But still there are many problems demands to solve such as at present: excrement cleaning technology is not perfect, subsequent processing
It is difficult；Mechanization degree is low, it is difficult to scale；Processing equipment structure is complex, and project investment is big, and operating cost is high, stink
Greatly, resource can utilize low.
Summary of the invention
It is an object of the invention to be directed to the deficiencies in the prior art, a kind of microbial flora provided handles livestock and poultry
The method of excrement, this method carries out fermentation process to feces of livestock and poultry using microbial flora, in entire fermentation process feces of livestock and poultry
Deodorization, fly eradication maggot effect it is good, it is energy conservation and environmental protection, free from environmental pollution, and this method processing step is simple, it is easily operated, substantially
The processing cost of degree reduced in aquaculture industry to feces of livestock and poultry, substantially increases the surcharge and utilization of feces of livestock and poultry
Rate.
To achieve the above object, the present invention adopts the following technical scheme:
A kind of method of microbial flora processing feces of livestock and poultry, comprising the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:1-3 in tobacco
Molasses are added in leftover bits and pieces to be uniformly mixed, it is spare to obtain material A；
(2) feces of livestock and poultry to be processed is collected in excrement storehouse, according to feces of livestock and poultry: material A is the weight ratio of 10:3-4
Material A is added in feces of livestock and poultry to be uniformly mixed, obtains mixed material B；It is equal that microbial flora mixing is added in mixed material B
Even, the additional amount of microbial flora is the 0.5-0.8% of mixed material B weight, obtains mixed material C；
(3) it after the biodiversity content for adjusting mixed material C is 55-70%, is placed in fermentation bed, the height of fermentation heap is 1-
1.8m, width 0.8-1.2m carry out temperature measurement to fermentation materials, when fermentation materials temperature reaches 70- during the fermentation
At 75 DEG C, by turner to material turn over throwing (by turn over throw the temperature that can reduce material and the material on surface it is buried
Bottom), the temperature of fermentation material is controlled at 65-75 DEG C；
(4) after fermenting 10-15 days, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature is basically stable at 65
DEG C or less no longer rise when；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and piles a rule height and is less than
Material is spread out the weight moisture capacity dried to material less than 25% after continuing fermentation 3-5 days by the pile of 20cm, is obtained fermentation and is produced
Then tunning is crushed, is sieved, obtains finished product after metering packing by object.
The method of above-mentioned microbial flora processing feces of livestock and poultry, the tobacco leftovers include that cigarette process is useless
Tobacco leaf, tobacco rod and the tobacco powder of abandoning；The feces of livestock and poultry is pig manure, cow dung, sheep dung, chicken manure, duck excrement, goose excrement, rabbit excrement and dove
One of excrement is a variety of.
The method of above-mentioned microbial flora processing feces of livestock and poultry in the step (2), is added micro- in mixed material B
Biological flora is after mixing, moreover it is possible to EM bacterium further be added and ferment together after mixing, the dosage of EM bacterium is microbial bacteria
The 1/3 of group's dosage.
In the method for above-mentioned microbial flora processing feces of livestock and poultry, the cultural method of the microbial flora include with
Lower step:
(1) rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain,
It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0-4 DEG C, obtain original flora；It is described
Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not
Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with
20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original
The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain
The brook bank of the wooden mixed forest zones obtains；
(2) rotted leaf containing original flora and soil are taken out into a part, grinds to obtain mud in desinfection chamber；
(3) mud containing original flora is added to the double distilled water of 10 times of weight, is uniformly mixed, is dispensed into several transparent glass
Glass culture bottle stands 15-30 days under conditions of 25-35 DEG C indoors, discards muddy and smelly water culture bottle, water energy is reached
Become as clear as crystal culture bottle, filter out the clear water of the inside, be put into Clear glass bottles and jars, then observe 15-30 days, obtains still
So as clear as crystal, not smelly clear water is strain A, spare；
(4) distilled water, the in parts by weight fresh mucus inside addition 2-3% chitterlings, the chicken fermented are added in vial
Excrement and pig manure dry powder 2-5%, 2-3% plain chocolate, 3-5% starch, 2-3% molasses, 2-5% soy meal, 2-5% fresh white dish leaf and 2-5%
Livestock meat powder, remaining is distilled water, is stirred evenly, and covers under conditions of 25-40 DEG C place 25-30 days indoors, keeps its rotten
It is rotten to go bad smelly, its smelly solution is taken, it is spare；
(5) by the obtained strain A of (3) step；The double distilled water dilution of 10 times of weight is added, is dispensed into several 100 ml's
In transparent glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml of (4) step, is uniformly mixed, adds ventilating cover,
It cultivates 15-30 days under conditions of 20-35 DEG C indoors, observes the solution of transparent glass culture bottle, take that it is still as clear as crystal, no
Smelly culture bottle solution is strain B, spare；
(6) culture medium by microwave disinfection is added in strain B 25-35 DEG C of condition expands culture 15-30 days indoors,
Obtain microbial flora；The composition weight content of the culture medium is as follows:
Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate
0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water
100mL, pH 7.2-7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, point
At 121 DEG C after dress, high pressure sterilization is obtained under conditions of 20min.
Used molasses are remaining molasses or beet, sweet tea after cane sugar factory crystallization of sucrose white sugar in the present invention
Remaining molasses after kaoliang stalk sugaring crystallization white sugar, water is not added in molasses, and places in a sealed container.
The method of microbial flora processing feces of livestock and poultry of the present invention, what feces of livestock and poultry obtained after fermentation process
Finished product is as farmyard manure directly using or for making organic fertilizer.
Tobacco leftovers used in the present invention include tobacco leaf, tobacco rod and the tobacco powder discarded in cigarette process.Tobacco
It is a kind of important industrial crops, tobacco leaf is only used as raw material of cigarette by tobacco resource very abundant, but purposes is single, and accounting for
The root of biological tobacco yield half, stem, wooden fork, top, leaf (not applicable tobacco leaf) cause very big waste as offal treatment.
Containing largely for the protein, sugar, rouge and the nicotine that utilize in these wastes, organic matter rich in, amino acid, biology
The elements such as potassium are the fabulous raw materials for manufacturing various organic fertilizers.Tobacco leftovers are subjected to fermentation and prepare organic fertilizer, are obtained
Organic fertilizer can supplement soil organic fertilizer, nutrient needed for slow release plant growth upon application；It can promote beneficial
Microbial reproduction, fast decoupled soil nutrient (N, P, K and microelement) make soil restoration vigor, increase soil water-retaining, fertilizer conservation
Ability.Particular matter such as nicotine also known as nicotine energy desinsection expelling parasite in tobacco, effectively expeling subterranean pest-insect；Tannin can be sterilized and be removed
Mite inhibits soil-borne disease, effectively prevents underground nematode;Phenol, aldehyde, quinone, lipid aromatic substance and organic acid: belong to natural plants growth
Regulator.Tobacco leftovers are added in the present invention to ferment together with feces of livestock and poultry, can effectively inhibit to bring in fermentation process
Fly mosquito, reduce fermentation maturity smell, and the nutrient of finished product fertilizer can be increased, turn waste into wealth, moreover it is possible to greatly increase
The anthelmintic action of finished product fertilizer.
The invention has the benefit that
1, the fermentative microflora used in the present invention is to breed to obtain by screening and scientific method from the Nature, is had superpower
Breeding and adaptability, for the fermentative microflora in composting fermentation process, microorganism in fermentative microflora can decompose feces of livestock and poultry
Middle organic substance, organic sulfur compound, the organic nitrogen etc. for generating foul gas, the stink that can be effectively removed in feces of livestock and poultry,
The generation of the stinks such as ammonia is reduced, and inhibits the growth of putrefactive microorganisms, substantially improves the air regime of Workplace.Entire hair
Pass through turner during ferment and turn over and throw the temperature that can the reduce material and material on surface is buried in bottom, allows entire material
It ferments more abundant, is safer, so that feces of livestock and poultry to be made to harmless biological organic waste material.And in the material after fermenting
Containing a large amount of beneficial organism bacterium, material after fermentation is used as fertilizer, these beneficial organism bacterium high survival rate in the soil can have
Quantity of useful microbe in the increase soil of effect improves soil activating character, repairs contaminated soil, purifies home environment,
Soil fertilizer, water conservation are improved, cold tolerance increases the available nutrient in soil, and breeding forms profitable strain enhancing crop rapidly
Disease resistance reduces the pest and disease damage phenomenon of crop.
2, the present invention during handle feces of livestock and poultry using tobacco leftovers (including discard tobacco leaf, tobacco rod and
Tobacco powder etc.) it is mixed together fermentation, the special smell of tobacco leftovers and its particular matter such as nicotine contained etc. can effectively press down
Bring fly mosquito in fermentation process processed reduces fermentation maturity smell, substantially improves the environment of fermentation.And use tobacco
Leftover bits and pieces ferments together can increase the nutrient of finished product fertilizer, turn waste into wealth, moreover it is possible to greatly increase the anthelmintic action of finished product fertilizer.
3, fermentation process is carried out to animal waste using method of the invention, in removing for entire fermentation process feces of livestock and poultry
It is smelly, fly eradication maggot effect is good, energy conservation and environmental protection, free from environmental pollution, by fermentation process, harmful pathogenic microorganism in excrement is had
Effect is killed, and after everfermentation, fermentation materials are converted to brown, and quality is loose, without the exclusive stink of excrement.The present invention is to cigarette
Careless leftover bits and pieces and feces of livestock and poultry, which carry out fermentation process, can mitigate the pollution on the environments such as waste, protect ecological ring
Border, and the purpose of utilization of waste as resource is realized, turn waste into wealth, it is environmentally protective.Microbial flora of the present invention handles livestock and poultry
The method and process step of excrement is simple, easily operated, significantly reduces the processing cost in aquaculture industry to feces of livestock and poultry,
Substantially increase the surcharge and utilization rate of feces of livestock and poultry.The finished product that feces of livestock and poultry of the present invention obtains after fermentation process is made
It is farmyard manure directly using or for making organic fertilizer.
Specific embodiment
Embodiment 1
A kind of method of microbial flora processing feces of livestock and poultry, comprising the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:1 under tobacco
Molasses are added in heel to be uniformly mixed, it is spare to obtain material A；The tobacco leftovers include the discarded cigarette of cigarette process
Leaf, tobacco rod and tobacco powder；
(2) chicken manure to be processed is collected in excrement storehouse, according to chicken manure: the weight ratio that material A is 10:4 adds in chicken manure
Enter material A to be uniformly mixed, obtains mixed material B；Microbial flora is added in mixed material B to be uniformly mixed, microbial flora
Additional amount be the 0.5% of mixed material B weight, obtain mixed material C；
(3) it after the biodiversity content for adjusting mixed material C is 55%, is placed in fermentation bed, the height of fermentation heap is 1m, width
Temperature measurement is carried out to fermentation materials during the fermentation for 0.8m, when fermentation materials temperature reaches 70 DEG C, is thrown by turning over
Machine carries out material to turn over throwing, controls the temperature of fermentation material at 65-75 DEG C；
(4) ferment 10 days after, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature be basically stable at 65 DEG C with
Under when no longer rising；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and pile a rule height less than 20cm's
Material is spread out the weight moisture capacity dried to material less than 25%, obtains tunning, then by pile after continuing fermentation 5 days
Tunning is crushed, sieved, obtains finished product after metering packing.
The cultural method of the microbial flora the following steps are included:
(1) rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain,
It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0-4 DEG C, obtain original flora；It is described
Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not
Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with
20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original
The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain
The brook bank of the wooden mixed forest zones obtains；
(2) rotted leaf containing original flora and soil are taken out into a part, grinds to obtain mud in desinfection chamber；
(3) mud containing original flora is added to the double distilled water of 10 times of weight, is uniformly mixed, is dispensed into several transparent glass
Glass culture bottle stands 30 days under conditions of 25 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear
Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 15 days, obtains still limpid
Bright, not smelly clear water is strain A, spare；
(4) distilled water is added in vial, the fresh mucus inside 2% chitterlings, the chicken manure to ferment are added in parts by weight
With pig manure dry powder 5%, 2% plain chocolate, 5% starch, 2% molasses, 5% soy meal, 2% fresh white dish leaf and 5% livestock meat powder, remaining
It for distilled water, stirs evenly, covers under conditions of 25 DEG C place 30 days indoors, so that it is rotted smelly, take its smelly molten
Liquid, it is spare；
(5) by the obtained strain A of (3) step；The double distilled water dilution of 10 times of weight is added, is dispensed into several 100 ml's
In transparent glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml of (4) step, is uniformly mixed, adds ventilating cover,
It cultivates 30 days under conditions of 20 DEG C indoors, observes the solution of transparent glass culture bottle, take it still as clear as crystal, not smelly
Culture bottle solution is strain B, spare；
(6) culture medium by microwave disinfection is added in strain B 25 DEG C of condition expands culture 30 days indoors, obtained micro-
Biological flora；The composition weight content of the culture medium is as follows:
Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate
0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water
100mL, pH 7.2-7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, point
At 121 DEG C after dress, high pressure sterilization is obtained under conditions of 20min.
Embodiment 2
A kind of method of microbial flora processing feces of livestock and poultry, comprising the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:2 under tobacco
Molasses are added in heel to be uniformly mixed, it is spare to obtain material A；The tobacco leftovers include the discarded cigarette of cigarette process
Leaf, tobacco rod and tobacco powder；
(2) feces of livestock and poultry (pig manure and cow dung) to be processed is collected in excrement storehouse, according to feces of livestock and poultry: material A 10:
Material A is added in feces of livestock and poultry and is uniformly mixed for 3.5 weight ratio, obtains mixed material B；It is added in mixed material B micro-
Biological flora is uniformly mixed, and the additional amount of microbial flora is the 0.7% of mixed material B weight, obtains mixed material C；
(3) it after the biodiversity content for adjusting mixed material C is 60%, is placed in fermentation bed, the height of fermentation heap is
1.5m, width 1m carry out temperature measurement to fermentation materials, when fermentation materials temperature reaches during the fermentation
When to 72 DEG C, material is carried out by turner to turn over throwing, controls the temperature of fermentation material at 65-75 DEG C；
(4) ferment 12 days after, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature be basically stable at 65 DEG C with
Under when no longer rising；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and pile a rule height less than 20cm's
Material is spread out the weight moisture capacity dried to material less than 25%, obtains tunning, then by pile after continuing fermentation 4 days
Tunning is crushed, sieved, obtains finished product after metering packing.
The cultural method of the microbial flora the following steps are included:
(1) rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain,
It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0-4 DEG C, obtain original flora；It is described
Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not
Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with
20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original
The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain
The brook bank of the wooden mixed forest zones obtains；
(2) rotted leaf containing original flora and soil are taken out into a part, grinds to obtain mud in desinfection chamber；
(3) mud containing original flora is added to the double distilled water of 10 times of weight, is uniformly mixed, is dispensed into several transparent glass
Glass culture bottle stands 25 days under conditions of 30 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear
Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 20 days, obtains still limpid
Bright, not smelly clear water is strain A, spare；
(4) distilled water is added in vial, the fresh mucus inside 3% chitterlings, the chicken manure to ferment are added in parts by weight
With pig manure dry powder 2%, 3% plain chocolate, 3% starch, 3% molasses, 2% soy meal, 5% fresh white dish leaf and 2% livestock meat powder, remaining
It for distilled water, stirs evenly, covers under conditions of 35 DEG C place 28 days indoors, so that it is rotted smelly, take its smelly molten
Liquid, it is spare；
(5) by the obtained strain A of (3) step；The double distilled water dilution of 10 times of weight is added, is dispensed into several 100 ml's
In transparent glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml of (4) step, is uniformly mixed, adds ventilating cover,
It cultivates 20 days under conditions of 30 DEG C indoors, observes the solution of transparent glass culture bottle, take it still as clear as crystal, not smelly
Culture bottle solution is strain B, spare；
(6) culture medium by microwave disinfection is added in strain B 30 DEG C of condition expands culture 25 days indoors, obtained micro-
Biological flora；The composition weight content of the culture medium is as follows:
Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate
0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water
100mL, pH 7.2-7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, point
At 121 DEG C after dress, high pressure sterilization is obtained under conditions of 20min.
Embodiment 3
A kind of method of microbial flora processing feces of livestock and poultry, comprising the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:3 under tobacco
Molasses are added in heel to be uniformly mixed, it is spare to obtain material A；The tobacco leftovers include the discarded cigarette of cigarette process
Leaf, tobacco rod and tobacco powder；
(2) feces of livestock and poultry (sheep dung, duck excrement and goose excrement) to be processed is collected in excrement storehouse, according to feces of livestock and poultry: material A is
Material A is added in feces of livestock and poultry and is uniformly mixed for the weight ratio of 10:3, obtains mixed material B；It is added in mixed material B micro-
Biological flora is uniformly mixed, and the additional amount of microbial flora is the 0.8% of mixed material B weight, obtains mixed material C；
(3) it after the biodiversity content for adjusting mixed material C is 70%, is placed in fermentation bed, the height of fermentation heap is 1.8m, wide
Degree is 1.2m, during the fermentation, temperature measurement is carried out to fermentation materials, when fermentation materials temperature reaches 75 DEG C, by turning over
Throwing machine carries out material to turn over throwing, controls the temperature of fermentation material at 65-75 DEG C；
(4) ferment 15 days after, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature be basically stable at 65 DEG C with
Under when no longer rising；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and pile a rule height less than 20cm's
Material is spread out the weight moisture capacity dried to material less than 25%, obtains tunning, then by pile after continuing fermentation 3 days
Tunning is crushed, sieved, obtains finished product after metering packing.
The cultural method of the microbial flora the following steps are included:
(1) rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain,
It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0-4 DEG C, obtain original flora；It is described
Dayao Mountain high mountain virgin forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also not
Through herding；Guangxi Zhuang Autonomous Region Yao Autonomous County of Jinxiu shrine mountain is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, with
20 kilometers of county town linear distance, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, containing original
The rotted leaf and soil of flora are weeds, coniferous forest, broad-leaf forest and the filling from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain
The brook bank of the wooden mixed forest zones obtains；
(2) rotted leaf containing original flora and soil are taken out into a part, grinds to obtain mud in desinfection chamber；
(3) mud containing original flora is added to the double distilled water of 10 times of weight, is uniformly mixed, is dispensed into several transparent glass
Glass culture bottle stands 15 days under conditions of 35 DEG C indoors, discards muddy and smelly water culture bottle, water energy, which is reached, becomes clear
Clear transparent culture bottle filters out the clear water of the inside, is put into Clear glass bottles and jars, then observe 30 days, obtains still limpid
Bright, not smelly clear water is strain A, spare；
(4) distilled water is added in vial, the fresh mucus inside 2.5% chitterlings, the chicken fermented are added in parts by weight
Excrement and pig manure dry powder 3%, 2.5% plain chocolate, 4% starch, 2.5% molasses, 3% soy meal, 4% fresh white dish leaf and 3% livestock meat are broken
End, remaining is distilled water, is stirred evenly, and covers under conditions of 40 DEG C place 25 days indoors, so that it is rotted smelly, take it
Smelly solution, it is spare；
(5) by the obtained strain A of (3) step；The double distilled water dilution of 10 times of weight is added, is dispensed into several 100 ml's
In transparent glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml of (4) step, is uniformly mixed, adds ventilating cover,
It cultivates 15 days under conditions of 35 DEG C indoors, observes the solution of transparent glass culture bottle, take it still as clear as crystal, not smelly
Culture bottle solution is strain B, spare；
(6) culture medium by microwave disinfection is added in strain B 35 DEG C of condition expands culture 15 days indoors, obtained micro-
Biological flora；The composition weight content of the culture medium is as follows:
Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate
0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water
100mL, pH 7.2-7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, point
At 121 DEG C after dress, high pressure sterilization is obtained under conditions of 20min.
Embodiment 4
A kind of method of microbial flora processing feces of livestock and poultry, comprising the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:2.5 in tobacco
Molasses are added in leftover bits and pieces to be uniformly mixed, it is spare to obtain material A；The tobacco leftovers include the discarded cigarette of cigarette process
Leaf, tobacco rod and tobacco powder；
(2) feces of livestock and poultry to be processed is collected in excrement storehouse, the feces of livestock and poultry is pig manure, cow dung, sheep dung, chicken manure, duck
One of excrement, goose excrement, rabbit excrement and pigeon dung are a variety of；According to feces of livestock and poultry: material A is the weight ratio of 10:4 in animal dung
Just material A is added in be uniformly mixed, obtains mixed material B；Microbial flora is added in mixed material B and the mixing of EM bacterium is equal
Even, the additional amount of microbial flora is that the additional amount of 0.6%, the EM bacterium of mixed material B weight is the 1/3 of microbial flora dosage,
Obtain mixed material C；The cultural method of the microbial flora is consistent in embodiment 1；
(3) it after the biodiversity content for adjusting mixed material C is 65%, is placed in fermentation bed, the height of fermentation heap is 1.2m, wide
Degree is 1m, during the fermentation, temperature measurement is carried out to fermentation materials, when fermentation materials temperature reaches 70-75 DEG C, by turning over
Throwing machine carries out material to turn over throwing, controls the temperature of fermentation material at 65-75 DEG C；
(4) ferment 15 days after, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature be basically stable at 65 DEG C with
Under when no longer rising；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and pile a rule height less than 20cm's
Material is spread out the weight moisture capacity dried to material less than 25%, obtains tunning, then by pile after continuing fermentation 4 days
Tunning is crushed, sieved, obtains finished product after metering packing.
Claims (8)
1. a kind of method of microbial flora processing feces of livestock and poultry, which comprises the following steps:
(1) tobacco leftovers are crushed, then according to tobacco leftovers: molasses are the weight ratio of 100:1-3 in tobacco
Molasses are added in leftover bits and pieces to be uniformly mixed, it is spare to obtain material A；
(2) feces of livestock and poultry to be processed is collected in excrement storehouse, according to feces of livestock and poultry: material A is the weight ratio of 10:3-4
Material A is added in feces of livestock and poultry to be uniformly mixed, obtains mixed material B；It is equal that microbial flora mixing is added in mixed material B
Even, the additional amount of microbial flora is the 0.5-0.8% of mixed material B weight, obtains mixed material C；
(3) it after the biodiversity content for adjusting mixed material C is 55-70%, is placed in fermentation bed, the height of fermentation heap is 1-
1.8m, width 0.8-1.2m carry out temperature measurement to fermentation materials, when fermentation materials temperature reaches 70- during the fermentation
At 75 DEG C, material is carried out by turner to turn over throwing, controls the temperature of fermentation material at 65-75 DEG C；
(4) after fermenting 10-15 days, temperature measurement is carried out again to fermentation materials, until fermentation materials temperature is basically stable at 65
DEG C or less no longer rise when；Fermentation materials are subjected to turning with pile self-propelled stack turning machine, and piles a rule height and is less than
Material is spread out the weight moisture capacity dried to material less than 25% after continuing fermentation 3-5 days by the pile of 20cm, is obtained fermentation and is produced
Then tunning is crushed, is sieved, obtains finished product after metering packing by object.
2. the method for microbial flora processing feces of livestock and poultry according to claim 1, which is characterized in that under the tobacco
Heel includes discarded tobacco leaf, tobacco rod and the tobacco powder of cigarette process.
3. the method for microbial flora processing feces of livestock and poultry according to claim 1, which is characterized in that the feces of livestock and poultry
For one of pig manure, cow dung, sheep dung, chicken manure, duck excrement, goose excrement, rabbit excrement and pigeon dung or a variety of.
4. the method for microbial flora processing feces of livestock and poultry according to claim 1, which is characterized in that the microorganism
The cultural method of flora the following steps are included:
(1) rotted leaf and soil that weight is each 50% are extracted by the ditch that virgin forest is fallen leaves more on Dayaoshan, Guangxi high mountain,
It seals up for safekeeping in the Brown Glass Brown glass bottles and jars only sterilized, brings back the interior preservation of refrigerator that laboratory is placed in 0-4 DEG C, obtain original flora；
(2) rotted leaf containing original flora and soil are taken out into a part, grinds to obtain mud in desinfection chamber；
(3) mud containing original flora is added to the double distilled water of 10 times of weight, is uniformly mixed, is dispensed into several transparent glass
Glass culture bottle stands 15-30 days under conditions of 25-35 DEG C indoors, discards muddy and smelly water culture bottle, water energy is reached
Become as clear as crystal culture bottle, filter out the clear water of the inside, be put into Clear glass bottles and jars, then observe 15-30 days, obtains still
So as clear as crystal, not smelly clear water is strain A, spare；
(4) distilled water, the in parts by weight fresh mucus inside addition 2-3% chitterlings, the chicken fermented are added in vial
Excrement and pig manure dry powder 2-5%, 2-3% plain chocolate, 3-5% starch, 2-3% molasses, 2-5% soy meal, 2-5% fresh white dish leaf and 2-5%
Livestock meat powder, remaining is distilled water, is stirred evenly, and covers under conditions of 25-40 DEG C place 25-30 days indoors, keeps its rotten
It is rotten to go bad smelly, its smelly solution is taken, it is spare；
(5) by the obtained strain A of (3) step；The double distilled water dilution of 10 times of weight is added, is dispensed into several 100 ml's
In transparent glass culture bottle, each 100 ml culture bottle instills the smelly solution 5ml of (4) step, is uniformly mixed, adds ventilating cover,
It cultivates 15-30 days under conditions of 20-35 DEG C indoors, observes the solution of transparent glass culture bottle, take that it is still as clear as crystal, no
Smelly culture bottle solution is strain B, spare；
(6) culture medium by microwave disinfection is added in strain B 25-35 DEG C of condition expands culture 15-30 days indoors,
Obtain microbial flora.
5. the cultural method of microbial flora according to claim 4, it is characterised in that: the Dayao Mountain high mountain is original
Forest be weeds, coniferous forest, broad-leaf forest and shrub mixed forest, not by artificial pollution, also without herding；Zhuang nationality in Guangxi
Autonomous region, shrine mountain, Yao Autonomous County of Jinxiu is located at Guangxi Gold show county town southwest, the also known as mountain Sheng Tang, public with county town linear distance 20
In, 45 kilometers of highway distance, 150 square kilometres of the gross area, 1979 meters of top height above sea level, rotted leaf and soil containing original flora
Be the weeds from 1000 meters to 1500 meters of hill-side height above sea level on shrine mountain, coniferous forest, broad-leaf forest and shrub mixed forest zones brook
Side obtains.
6. the cultural method of microbial flora according to claim 4, it is characterised in that: the culture of described (6) step
The composition weight content of base is as follows:
Beef extract 1.0g, peptone 1.0g, molasses 1.0g, starch 1.0g, urea 1.0g, Sodium lysinate 0.5g, sodium glutamate
0.5g, vitamin B 0.5g, potassium dihydrogen phosphate 1.0g, sodium chloride 0.5g, magnesium chloride 0.5g, yeast extract 1.0g, distilled water
100mL, pH 7.2-7.5；The culture medium preparation method is: each raw material is placed in triangular flask, pH value is adjusted in heating and dissolution, point
At 121 DEG C after dress, high pressure sterilization is obtained under conditions of 20min.
7. the method for microbial flora processing feces of livestock and poultry according to claim 1, which is characterized in that the step (2)
In, microbial flora is added after mixing in mixed material B, moreover it is possible to the starting after mixing of EM bacterium further be added
Ferment.
8. the method for microbial flora processing feces of livestock and poultry according to claim 1, which is characterized in that feces of livestock and poultry passes through
The finished product obtained after fermentation process is as farmyard manure directly using or for making organic fertilizer.
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| CN113278527A (en) * | 2021-05-20 | 2021-08-20 | 秋路 | Preparation method of microbial decomposition material |
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