CN109758611B - Method for preparing active biological tissue engineering scaffold by solvent spraying - Google Patents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/06—Wet spinning methods
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/12—Stretch-spinning methods
Abstract
The technical scheme discloses a solvent-spraying preparation method of an active biological tissue engineering scaffold, which is characterized by comprising the following steps: (1) dissolving a biomedical material to obtain a biomedical material water solution with the concentration of 2-50%; (2) adding an immunoglobulin solution into the biomedical material aqueous solution prepared in the step (1) to prepare an active spinning solution; (3) carrying out melt-blown spinning by taking a high-pressure air jet flow with the temperature of 0-37 ℃ as stretching power, and receiving the spinning by using a spinning receiver; (4) and (3) arranging a cell printing device behind the fiber receiver, printing an active solution containing seed cells or cell growth factors into the fiber membrane prepared in the step (3), and finally preparing the active biological tissue engineering scaffold. The active biological tissue engineering scaffold prepared by the technical scheme can be implanted into a human body after proper in vitro culture to be used as a biological tissue engineering scaffold or a skin tissue engineering scaffold to promote wound healing.
Description
Technical Field
The invention belongs to the technical field of biomedical materials, and particularly relates to a method for preparing a biological tissue engineering scaffold by spray.
Background
The loss or failure of human tissues and organs poses a serious threat to health and life. The use of materials and biotechnology to improve self-health has long been continuously explored and studied by humans. In 1993, the chemical engineers Langer R at the american college of science and technology of the massachusetts and the physician vacatti JP at the medical college of harvard university systematically proposed the idea of tissue engineering, i.e., the planting of living human cells on scaffolds to produce tissues under the action of growth factors, and the concept of tissue engineering.
The basic principle and method of tissue engineering is to plant in vitro expanded autologous or allogeneic cells into extracellular matrix mimics (scaffolds) constructed in vitro to form cell/scaffold complexes. Then the cell-scaffold compound is implanted into the damaged tissue or organ part, and new tissue or organ with the mechanism and function consistent with that of the target tissue or organ is formed through proliferation and differentiation of implanted cells and degradation and absorption matched with the extracellular matrix-like scaffold, so that the aims of wound repair and function reconstruction are fulfilled.
The research content of tissue engineering mainly focuses on the cultivation of seed cells, the development of tissue engineering scaffold, and the tissue engineering tissue or organ of cell/scaffold composite member. The tissue engineering scaffold provides a proper environment for the growth of cells and tissues, gradually degrades and disappears along with the division of the cells, thereby providing new space for the tissues and the cells and enabling newly generated tissues and organs to have the same geometric shape as the cell scaffold. The main roles of the scaffold in building a tissue-engineered tissue or organ include: (1) provide physical support for cell adhesion and accurate delivery of cells to the site of injury; (2) providing space for the proliferation and metabolism of cells; (3) providing specific macro and micro structures to guide the cell structure to a specific functional tissue or organ; (4) transmitting chemical or mechanical signals to regulate the phenotype of the cell. The design and construction of tissue engineering scaffolds involve three scales, namely, macroscopical (over centimeter scale), i.e., appearance, microscopic pore size, porosity, scaffold surface topology (microscale scale), scaffold surface adhesion proteins, and the effect of genes on cells (nanoscale scale). The ideal tissue engineering scaffold has the following basic requirements: (1) good cell compatibility; besides meeting the general requirements of biological materials such as no toxicity, no teratogenesis, no toxic effect of degradation products on cells and no inflammatory reaction, the biological material is also beneficial to the adhesion and proliferation of seed cells, and more importantly, the biological material can activate the specific gene expression of the cells and maintain the phenotype expression of the cells; (2) good biodegradability; the scaffold material can be degraded after the damaged tissue is repaired, and the degradation speed is matched with the tissue regeneration speed; (3) the porous material has a three-dimensional porous structure, and has proper pore diameter, high porosity and large specific surface area; (4) the scaffold has proper mechanical strength which is matched with the repaired tissue, provides support for the new tissue and can maintain the original shape of the tissue and the organ; (5) to prevent infection, stents must also be easily sterilized and stored.
The nanofiber is widely applied to medical fields of drug controlled release, artificial skin, wound healing, tooth enhancement and the like due to the high specific surface area and porous structure, but the fiber membranes for tissue engineering scaffolds on the market are all prepared by an electrostatic spinning technology. The basic principle of electrostatic spinning is as follows: the precursor solution is charged with high-voltage static electricity, and the charged polymer liquid drops are accelerated at the Taylor cone vertex of the capillary under the action of an electric field; when the electric field force is large enough, the polymer liquid drop can overcome the surface tension to form a jet stream; the fine stream evaporates or solidifies during the spraying process and eventually lands on a receiving device to form a fiber mat resembling a nonwoven fabric. The principle of electrostatic spinning shows that high-voltage static electricity is needed in electrostatic spinning, so that when the fiber membrane with biological activity is prepared, the biological activity of an active substance added in a spinning solution is influenced by the high-voltage static electricity, so that the biological activity of the prepared active biological tissue engineering scaffold is reduced, the non-woven fiber membrane prepared by the electrostatic spinning technology needs higher voltage, the fiber membrane has defects in operation safety and is not easy to be applied in industrial production in a large scale, and the electrostatic spinning production technology is just in the stage of entering industrial production, and a plurality of difficulties need to be overcome.
At present, most of the production of the non-woven fiber membranous tissue engineering scaffold is separated from preparation and cell culture, and cells are easy to grow on the surface of the scaffold.
Disclosure of Invention
The invention provides a solution-spraying preparation method of an active biological tissue engineering scaffold, which can simulate the animal spinning process in nature, and the biological tissue engineering scaffold prepared by the method is not easy to cause the growth of cells on the surface of the scaffold.
In order to achieve the purpose, the technical scheme adopts the following technical means.
A method for preparing a solvent-spray scaffold for active biological tissue engineering comprises the following steps:
(1) dissolving a biomedical material to obtain a biomedical material water solution with the concentration of 2-50%;
(2) adding an immunoglobulin solution into the biomedical material aqueous solution prepared in the step (1) to prepare an active spinning solution, wherein the concentration of immunoglobulin in the immunoglobulin solution is 1-10%;
(3) carrying out solution-jet spinning by taking a high-pressure air jet flow with the temperature of 0-37 ℃ as stretching power, and receiving the spinning by using a spinning receiver to prepare a fiber support;
(4) and (3) arranging a cell printing device behind the fiber receiver, printing the active solution containing the seed cells or the cell growth factors into the fiber scaffold prepared in the step (3), and finally preparing the active biological tissue engineering scaffold.
The preparation method of the active biological tissue engineering scaffold by solvent spraying also comprises the following steps: and (3) adding inorganic nano powder or organic nano powder into the active spinning solution prepared in the step (2) and uniformly mixing to obtain the active spinning solution used in the step (3).
Further, the inorganic nano powder is one of hydroxyapatite, tricalcium phosphate and graphene.
Further, the organic nano powder is nanocrystalline cellulose.
Further, the biomedical material is one of chitosan, sodium alginate, polyvinyl alcohol, bacterial cellulose, polyethylene oxide, cellulose and polyethylene glycol.
Further, the high-pressure air jet flow in the step (3) is dried compressed air, and the relative humidity of the high-pressure air jet flow is 10% -100%.
Further, a radiation device is arranged above the spinning receiver in the step (3), and the radiation device performs radiation crosslinking on the spun yarns; radiation crosslinking is carried out on the spun yarn by adopting a radiation device, wherein the radiation intensity on the spun yarn is 1800mW/cm2Therefore, the crosslinking degree between biomacromolecules in the fiber can be improved, and the solubility of the obtained spinning fiber in an aqueous solution is reduced, so that the strength of the spinning fiber is improved.
Further, the radiation source of the radiation device is an ionizing radiation source, and comprises one of an X-ray radiation source, a beta-particle radiation source, an alpha-particle radiation source and a lambda-ray radiation source.
Further, when the spinning receiver adopts a spinning receiving flat plate, the active biological tissue engineering fiber membrane is obtained.
Further, when the spinning receiver adopts a spinning receiving roller, the active biological tissue engineering continuous long fiber membrane with the spinning fibers stretched unidirectionally and arranged unidirectionally and orderly is obtained.
Furthermore, the injection pressure of the high-pressure air injection flow is 10MPa to 30 MPa.
The beneficial effects of this technical scheme do: the preparation method in the technical scheme does not need voltage, and water is used as a solvent, so that the preparation method is easier to be applied in large scale in industrial production; the biological tissue engineering scaffold prepared by the preparation method in the technical scheme has a structure similar to that of an electrostatic spinning fibrous membrane and also has high porosity, the preparation method can conveniently adjust processing parameters, the requirement of cell growth on the porosity of a material is met, and the prepared biological tissue engineering scaffold can be ensured to have good pore canal connectivity by a structure formed by stacking nano fibers layer by layer; the fiber diameter of the biological tissue engineering scaffold prepared by the technical scheme is 100 nm-10 mu m, so that the structure of the human extracellular matrix can be simulated to the maximum extent; the biological tissue engineering scaffold prepared by the preparation method has large specific surface area, can provide a good microenvironment for the survival of cells, and is beneficial to the adhesion, differentiation and proliferation of the cells; the active fiber non-woven fabric implanted with the seed cells prepared by the method can be implanted into a human body after proper in vitro culture to be used as a biological tissue engineering scaffold or a skin tissue engineering scaffold to promote wound healing;
in the application, an immunoglobulin solution is added into a traditional spinning solution, and immunoglobulin (Ig) refers to globulin which has antibody activity or chemical structure and is similar to antibody molecules; immunoglobulins are divided into antibodies, which are mainly present in serum and whose main function is to specifically bind to an antigen, and membrane immunoglobulins; the membrane immunoglobulin is an antigen receptor on a B cell membrane and can specifically recognize antigen molecules; the immunoglobulin can activate complement, so compared with the traditional fibrous membrane, the active biological tissue engineering scaffold prepared by using the spinning solution added with the immunoglobulin is more favorable for the adhesion and proliferation of seed cells, and the cell growth factor can activate the specific gene expression of the cells and maintain the phenotype expression of the cells;
but the activity of the immunoglobulin is influenced by factors such as temperature, solution pH value and the like, and in the technical scheme, a high-pressure air jet flow with the temperature of 0-37 ℃ is used as stretching power to carry out melt-spinning, so that the activity of the immunoglobulin is not influenced at the temperature of 0-37 ℃; and when the temperature is higher than 37 ℃, the immunoglobulin loses activity due to overhigh temperature, so the technical scheme effectively keeps the activity of the immunoglobulin and the structural integrity of fiber spinning by carrying out the solution-jet spinning at 0-37 ℃.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The technical scheme of the invention is further explained by combining specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Example 1
A method for preparing active bioengineering scaffold by solvent spray comprises the following steps: (1) dissolving cellulose at-12 ℃ by using NaOH (7 wt%)/urea (12 wt%) aqueous solution as a solvent to obtain a cellulose solution with the concentration of 5 wt%; (2) adding nanocrystalline cellulose into a cellulose solution, and uniformly stirring to prepare a cellulose spinning solution; (3) adding an immunoglobulin solution with the concentration of 10% into the cellulose spinning solution prepared in the step (2) to prepare an active spinning solution; (4) taking a high-pressure air jet flow with the temperature of 37 ℃ and the relative humidity of 100% as stretching power, taking the jet pressure in the high-pressure air jet flow as 30MPa, carrying out melt-spinning on the active spinning solution, taking the spinning receiver as a spinning receiving flat plate, and obtaining the cellulose/nanocrystalline cellulose active biological tissue engineering scaffold, wherein the cell printing solution is bovine serum albumin aqueous solution containing fibroblast growth factors with the concentration of 5% and skin fibroblasts with the concentration of 20%; finally, the active biological tissue engineering scaffold of the technical scheme is obtained, and the average diameter of the fiber is 2 μm.
Example 2
A method for preparing active bioengineering scaffold by solvent spray comprises the following steps: (1) dissolving polyethylene oxide (with a relative molecular weight of 100 ten thousand) by using water as a solvent to obtain a 10 wt% polyethylene oxide aqueous solution; (2) adding the polyethylene oxide spinning solution prepared in the step (1)Preparing an active spinning solution from an immunoglobulin solution with the concentration of 10 percent; (3) carrying out solution-jet spinning on the polyethylene oxide spinning solution by taking a high-pressure air jet flow with the temperature of 37 ℃ and the humidity of 25 wt% as stretching power and the jet pressure in the high-pressure air jet flow of 25MPa, wherein a spinning receiver is a spinning receiving flat plate to obtain a fiber support; a lambda ray radiation device is arranged above the spinning receiving flat plate and is used for carrying out radiation crosslinking on the fibers, wherein the radiation intensity is 1800mW/cm2The solubility of the polyethylene oxide fiber in the aqueous solution is reduced, the fiber mechanical strength is improved, the cell printing liquid in the cell printing device is a bone morphogenetic protein aqueous solution containing transforming growth factor beta 3 with the concentration of 10% and articular cartilage cells with the concentration of 30%, and finally the active biological tissue engineering scaffold in the technical scheme is obtained, and the average diameter of the fiber is 500 nm.
Example 3
A method for preparing active bioengineering scaffold by solvent spray comprises the following steps: (1) dissolving polyvinyl alcohol (with a relative molecular weight of 10 ten thousand) by using water as a solvent to obtain a 50 wt% polyvinyl alcohol aqueous solution; (2) adding an immunoglobulin solution with the concentration of 15% into the polyvinyl alcohol spinning solution prepared in the step (1) to prepare an active spinning solution; (3) using a high-pressure air jet flow with the temperature of 37 ℃ and the humidity of 10 wt% as stretching power, using the jet pressure in the high-pressure air jet flow as 20MPa, carrying out melt-spinning on polyvinyl alcohol spinning solution, using a spinning receiver as a spinning receiving flat plate to obtain a melt-spun non-woven fibrous membrane, arranging a lambda-ray radiation device above the spinning receiving flat plate, and carrying out radiation crosslinking on fibers by using the lambda-ray radiation device, wherein the radiation intensity is 1800mW/cm2The solubility of polyvinyl alcohol solution spraying fiber in water solution is reduced, the mechanical strength of fiber is improved, cell printing liquid in a cell printing device is bovine serum albumin water solution containing fibroblast growth factor with the concentration of 1% and skin fibroblast with the concentration of 30%, and finally the non-woven fiber membrane in the technical scheme is provided, and the average diameter of the fiber is 200 nm.
While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the present invention is not limited to the embodiments, but various equivalent modifications and substitutions can be made without departing from the spirit of the present invention, and the equivalents and substitutions are intended to be included in the scope of the present invention as defined by the appended claims.
Claims (4)
1. A method for preparing a soluble spray of an active biological tissue engineering scaffold is characterized by comprising the following steps:
(1) dissolving the biomedical material to obtain a biomedical material water solution with the concentration of 2-50%;
(2) adding an immunoglobulin solution and inorganic nano powder or organic nano powder into the biomedical material aqueous solution prepared in the step (1) and uniformly mixing to prepare an active spinning solution, wherein the concentration of immunoglobulin in the immunoglobulin solution is 1-15%; the inorganic nano powder is one of hydroxyapatite, tricalcium phosphate and graphene; the organic nano powder is nanocrystalline cellulose;
(3) carrying out melt-blown spinning by taking a high-pressure air jet flow with the temperature of 0-37 ℃ as stretching power, wherein the jet pressure in the high-pressure air jet flow is 10-30 MPa, and a spinning receiver is adopted to receive spinning to prepare a fiber support; the high-pressure air jet flow is dried compressed air, and the relative humidity of the high-pressure air jet flow is 10% -100%;
(4) a radiation device is arranged above the spinning receiver and performs radiation crosslinking on the spun yarns, wherein the radiation intensity on the spun yarns is 1800mW/cm2；
(5) And (3) arranging a cell printing device behind the fiber receiver, printing the active solution containing the seed cells or the cell growth factors into the fiber scaffold prepared in the step (3), and finally preparing the active biological tissue engineering scaffold.
2. The method for preparing the active scaffold for biological tissue engineering according to claim 1, wherein the biomedical material is one of chitosan, sodium alginate, polyvinyl alcohol, bacterial cellulose, polyethylene oxide and polyethylene glycol.
3. The method for preparing the scaffold for active biological tissue engineering according to claim 1, wherein the spinning receiver is a spinning receiving plate, and an active biological tissue engineering fibrous membrane is obtained.
4. The method for preparing the scaffold according to claim 1, wherein the spinning receiver is a spinning receiving roller, and the continuous filament membrane of active biological tissue engineering with the unidirectionally stretched and unidirectionally and orderly arranged spun fibers is obtained.
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| CN201811654045.5A CN109758611B (en) | 2018-12-28 | 2018-12-28 | Method for preparing active biological tissue engineering scaffold by solvent spraying |
| PCT/CN2019/113151 WO2020134445A1 (en) | 2018-12-28 | 2019-10-25 | Solution spray preparation method for scaffold for active biological tissue engineering |
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| CN201811654045.5A CN109758611B (en) | 2018-12-28 | 2018-12-28 | Method for preparing active biological tissue engineering scaffold by solvent spraying |
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| CN109758611B (en) * | 2018-12-28 | 2022-04-26 | 佛山科学技术学院 | Method for preparing active biological tissue engineering scaffold by solvent spraying |
| CN109529125B (en) * | 2018-12-28 | 2023-01-24 | 佛山科学技术学院 | Method for preparing biological tissue engineering scaffold by solvent spraying |
| CN110592686B (en) * | 2019-08-30 | 2020-06-26 | 河南亚都实业有限公司 | Growth factor loaded micro-nano composite membrane |
| CN112717191B (en) * | 2021-01-08 | 2021-11-16 | 齐齐哈尔大学 | Preparation method of customizable and functional dressing |
| CN114870070A (en) * | 2021-02-05 | 2022-08-09 | 诺一迈尔(苏州)生命科技有限公司 | Organic/inorganic composite three-dimensional porous nanofiber tissue engineering scaffold and preparation method and application thereof |
| CN115721780A (en) * | 2022-12-01 | 2023-03-03 | 国纳之星(上海)纳米科技发展有限公司 | Preparation method of burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, product and application thereof |
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