CN109529125B - Method for preparing biological tissue engineering scaffold by solvent spraying - Google Patents

Method for preparing biological tissue engineering scaffold by solvent spraying Download PDF

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Publication number
CN109529125B
CN109529125B CN201811632029.6A CN201811632029A CN109529125B CN 109529125 B CN109529125 B CN 109529125B CN 201811632029 A CN201811632029 A CN 201811632029A CN 109529125 B CN109529125 B CN 109529125B
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spinning
tissue engineering
biological tissue
engineering scaffold
pressure air
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CN109529125A (en
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罗杰
吕悦光
谢啸锋
林嘉栋
张敏
熊帮云
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Foshan University
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Foshan University
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Priority to PCT/CN2019/113150 priority patent/WO2020134444A1/en
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    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/724—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged forming webs during fibre formation, e.g. flash-spinning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/42—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
    • A61L27/422—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of carbon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/42—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
    • A61L27/425—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of phosphorus containing material, e.g. apatite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
    • D06M10/008—Treatment with radioactive elements or with neutrons, alpha, beta or gamma rays
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dermatology (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Textile Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Artificial Filaments (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The technical scheme discloses a method for preparing a biological tissue engineering scaffold by solvent spray, which comprises the following steps: (1) Dissolving the biomedical material to obtain a biomedical material water solution with the concentration of 2-50%; (2) And (3) carrying out solution-jet spinning by taking a high-pressure air jet as stretching power, and receiving the spinning by using a spinning receiver to prepare the biological tissue engineering scaffold. The fiber diameter of the biological tissue engineering scaffold prepared by the technical scheme is 100 nm-10 mu m, so that the structure of the human extracellular matrix can be simulated to the maximum extent; the biological tissue engineering scaffold prepared by the preparation method has large specific surface area, can provide a good microenvironment for the survival of cells, and is beneficial to the adhesion, differentiation and proliferation of the cells.

Description

Method for preparing biological tissue engineering scaffold by solvent spraying
Technical Field
The invention belongs to the technical field of biomedical materials, and particularly relates to a dissolving-bumping preparation method of a biological tissue engineering scaffold.
Background
The fibrous membrane in the biological tissue engineering scaffold can simulate the structure and function of extracellular matrix to a certain extent, provides ideal growth, proliferation and differentiation microenvironment for cells, and is widely applied to various fields including tissue engineering research of cartilage, bone, blood vessels, skin and the like within a few years. The electrostatic spinning technology is the most common preparation technology of the fiber membrane at present, the fiber membrane prepared by the electrostatic spinning technology needs higher voltage, has defects in operation safety and is not easy to be applied in industrial production in a large scale, and the electrostatic spinning production technology is just in the stage of entering the industrial production and has a plurality of difficulties to be overcome.
At present, most of the production of the fiber membranous tissue engineering scaffold is separated from cell culture, and cells are easy to grow on the surface of the scaffold.
Disclosure of Invention
The invention provides a method for preparing a biological tissue engineering scaffold by solvent spray, which can simulate the animal spinning process in the nature, and the biological tissue engineering scaffold prepared by the method is not easy to cause the growth of cells on the surface of the biological tissue engineering scaffold.
In order to achieve the purpose, the technical scheme adopts the following technical means.
A method for preparing a biological tissue engineering scaffold by spray comprises the following steps:
(1) Dissolving the biomedical material to obtain a biomedical material water solution with the concentration of 2-50%;
(2) And (3) carrying out melt-blown spinning by taking a high-pressure air jet as stretching power, and receiving the spinning by using a spinning receiver to prepare the biological tissue engineering scaffold.
Further, the injection pressure of the high-pressure air injection flow is 10MPa to 30MPa.
Further, the method for preparing the biological tissue engineering scaffold by the solvent spray method also comprises the following steps: and (2) adding inorganic nano powder or organic nano powder into the biomedical material aqueous solution prepared in the step (1), and uniformly mixing to obtain the spinning solution in the step (2).
Further, the inorganic nano powder is one of hydroxyapatite, tricalcium phosphate and graphene.
Further, the organic nano powder is nanocrystalline cellulose.
Further, the biomedical material is one of chitosan, sodium alginate, polyvinyl alcohol, polyethylene oxide, bacterial cellulose, cellulose and polyethylene glycol.
Further, the high-pressure air jet flow in the step (2) is dried compressed air, and the relative humidity of the high-pressure air jet flow is 10% -100%.
Further, a radiation device is arranged above the spinning receiver in the step (2), and the radiation device performs radiation crosslinking on the spun yarns; the radiation device is adopted to carry out radiation crosslinking on the spinning, so that the crosslinking degree among biomacromolecules in the fiber can be improved, the solubility of the obtained spinning fiber in aqueous solution is reduced, and the strength of the spinning fiber is improved.
Further, the radiation source of the radiation device is an ionizing radiation source, and comprises one of an X-ray radiation source, a beta-particle radiation source, an alpha-particle radiation source and a lambda-ray radiation source.
Further, when the spinning receiver adopts a spinning receiving flat plate, a fiber membrane is obtained.
Further, when the spinning receiver adopts a spinning receiving roller, the continuous long fiber film which is stretched unidirectionally and arranged unidirectionally is obtained.
The beneficial effects of this technical scheme do: the preparation method in the technical scheme does not need voltage, and water is used as a solvent, so that the preparation method is easier to be applied in large scale in industrial production; the biological tissue engineering scaffold prepared by the preparation method in the technical scheme has a structure similar to that of an electrostatic spinning fibrous membrane and also has high porosity, the preparation method can conveniently adjust processing parameters, the requirement of cell growth on the porosity of the material is met, and the prepared biological tissue engineering scaffold can be ensured to have good pore connectivity by a structure formed by stacking nano fibers layer by layer; the fiber diameter of the biological tissue engineering scaffold prepared by the technical scheme is 100 nm-10 mu m, so that the structure of the human extracellular matrix can be simulated to the maximum extent; the biological tissue engineering scaffold prepared by the preparation method has large specific surface area, can provide a good microenvironment for the survival of cells, and is beneficial to the adhesion, differentiation and proliferation of the cells.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The technical scheme of the invention is further explained by combining specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention can be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the claims appended to the present application.
Example 1
A method for preparing a biological tissue engineering scaffold by solvent spray comprises the following steps: (1) Dissolving cellulose at-12 ℃ by using NaOH (7 wt%)/urea (12 wt%) aqueous solution as a solvent to obtain 5wt% cellulose solution; (2) Adding nanocrystalline cellulose into a cellulose solution, and uniformly stirring; (3) And (2) carrying out solution-jet spinning on the cellulose/nanocrystalline cellulose spinning solution by using a high-pressure air jet flow with the temperature of 37 ℃ and the relative humidity of 100% as stretching power and the jet pressure of the high-pressure air jet flow of 30MPa, wherein the used fiber receiver is a fiber receiving flat plate, and carrying out radiation crosslinking on fibers by using a lambda ray radiation device above the fiber flat plate to finally obtain a fiber membrane, wherein the average diameter of the fibers in the prepared fiber membrane is 2 mu m.
Example 2
A method for preparing a biological engineering scaffold by spray comprises the following steps: (1) Dissolving poly (ethylene oxide) (relative molecular weight 100 ten thousand) by using water as a solvent to obtain 10wt% of polyethylene oxide aqueous solution; (2) And (2) carrying out spray spinning on the polyethylene oxide spinning solution by taking a high-pressure air jet flow with the temperature of 100 ℃ and the humidity of 25wt% as stretching power and the spray pressure of the high-pressure air jet flow of 25MPa to obtain a spray-dissolved fiber membrane, wherein the fiber receiver is a fiber receiving flat plate, and carrying out radiation crosslinking on fibers by using a lambda ray radiation device above the fiber receiver to reduce the solubility of the polyethylene oxide fibers in an aqueous solution, so as to finally obtain the fiber membrane, wherein the average diameter of the fibers in the prepared fiber membrane is 500nm.
Example 3
A method for preparing a biological engineering scaffold by spray comprises the following steps: (1) Dissolving polyvinyl alcohol (with a relative molecular weight of 10 ten thousand) by using water as a solvent to obtain a 50wt% polyvinyl alcohol aqueous solution; (2) And (2) carrying out spray spinning on the polyvinyl alcohol spinning solution by taking a high-pressure air jet flow with the temperature of 120 ℃ and the humidity of 10wt% as a stretching power and using the spray pressure of the high-pressure air jet flow as 20MPa to obtain a spray-dissolved fiber membrane, wherein the used fiber receiver is a fiber receiving flat plate, and carrying out radiation crosslinking on fibers by using a lambda ray radiation device above the fiber receiver to reduce the solubility of the polyvinyl alcohol spray-dissolved fibers in an aqueous solution, so as to finally obtain the wireless fiber membrane, wherein the average diameter of the fibers in the prepared fiber membrane is 200nm.
While the preferred embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that the present invention is not limited to the embodiments, but various equivalent modifications and substitutions can be made without departing from the spirit of the present invention, and the equivalents and substitutions are intended to be included in the scope of the present invention as defined by the appended claims.

Claims (4)

1. A method for preparing a biological tissue engineering scaffold by solvent spray is characterized by comprising the following steps:
(1) Dissolving a biomedical material to obtain a biomedical material water solution with the concentration of 2 to 50 percent;
(2) Adding inorganic nano powder or organic nano powder into the biomedical material aqueous solution prepared in the step (1) and uniformly mixing to obtain spinning solution; the inorganic nano powder is one of hydroxyapatite, tricalcium phosphate and graphene; the organic nano powder is nanocrystalline cellulose;
(3) Carrying out solution-jet spinning by taking a high-pressure air jet as stretching power, and receiving the spinning by using a spinning receiver to prepare the biological tissue engineering scaffold; the injection pressure of the high-pressure air injection flow is 10MPa to 30MPa; the high-pressure air jet flow is dried compressed air, and the relative humidity of the high-pressure air jet flow is 10% -100%;
the biomedical material is one of chitosan, sodium alginate, polyvinyl alcohol, cellulose and polyethylene glycol.
2. The method for preparing the scaffold for biological tissue engineering according to claim 1, wherein a radiation device is disposed above the spinning receiver in step (3), and the radiation device performs radiation crosslinking on the spun yarn.
3. The method for preparing the scaffold for biological tissue engineering according to claim 1, wherein the spinning receiver is a spinning receiving plate, and a fibrous membrane is obtained.
4. The method as claimed in claim 1, wherein the spinning receiver is a spinning receiving roller, and the continuous filament fiber film is stretched unidirectionally and arranged unidirectionally.
CN201811632029.6A 2018-12-28 2018-12-28 Method for preparing biological tissue engineering scaffold by solvent spraying Active CN109529125B (en)

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CN201811632029.6A CN109529125B (en) 2018-12-28 2018-12-28 Method for preparing biological tissue engineering scaffold by solvent spraying
PCT/CN2019/113150 WO2020134444A1 (en) 2018-12-28 2019-10-25 Preparation method for biological tissue engineering stent by solution spraying

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CN101775705B (en) * 2009-12-29 2011-04-27 东华大学 Manufacture method of biodegradable non-woven material
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CN109758611B (en) * 2018-12-28 2022-04-26 佛山科学技术学院 Method for preparing active biological tissue engineering scaffold by solvent spraying
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