CN109757481A - A kind of efficient disinfectant, preparation method and purposes for killing mycobacterium tuberculosis - Google Patents
A kind of efficient disinfectant, preparation method and purposes for killing mycobacterium tuberculosis Download PDFInfo
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- CN109757481A CN109757481A CN201910076219.2A CN201910076219A CN109757481A CN 109757481 A CN109757481 A CN 109757481A CN 201910076219 A CN201910076219 A CN 201910076219A CN 109757481 A CN109757481 A CN 109757481A
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- mycobacterium tuberculosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
A kind of efficient disinfectant, preparation method and purposes for killing mycobacterium tuberculosis, the invention discloses a kind of disinfectants for efficiently killing mycobacterium tuberculosis, mainly consist of the following compositions: levulic acid, neopelex etc..LAV-SDBS of the present invention can quickly and effectively kill mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus, it is sterilized for living body or non-living body, and it shows active to extensive microorganism, such as virus, fungi or bacterium, especially there is good disinfection effect to mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus, the air borne of mycobacterium tuberculosis can be cut off, and action speed is fast, effective time is long, it can be persistently antibacterial, it is without side-effects, there is good alternative and safety.
Description
Technical field: the present invention relates to disinfection technology field, specifically a kind of efficient disinfection for killing mycobacterium tuberculosis
Agent and its preparation method and application.The invention further relates to the applications that the disinfectant is sterilized for living body or non-living body, to knot
Core mycobacteria is particularly effective.
Background technique:
Disinfection is to reach innoxious requirement to kill pathogenic microorganism on communication media, by pathogenic microorganism eliminate in
Except human body, the route of transmission of infectious disease is cut off, achievees the purpose that control infectious disease.Currently used disinfectant have class containing iodine,
Class containing chlorine, aldehyde-containing type and peroxide.These disinfectants have good killing effect to microorganism but also there is difference
Side effect, such as the disinfectant of class containing iodine will lead to allergy, there are corrosivity, aldehyde-containing type disinfectants strong stimulation for the disinfectant of class containing chlorine
Property, toxicity and corrosivity, peroxide disinfectant can cause DNA damage, gene mutation, carcinogenic etc..
Mycobacterium tuberculosis mainly invades the low crowd of immunity of organisms or as continuing infection and with other diseases
Infection, so opportunistic pathogenesis is its distinguishing feature, mycobacterium tuberculosis is in that drug resistance often causes to general anti-tubercular drug more
Infection delay is refractory.The generation and propagation of substance of medicines-resistant branched tubercle bacillus bacterial strain, the appearance of especially multi-drug resistant bacterial strain, seriously draw
Play treatment cycle and effect, it has also become one of the problem of new century Tubercufosis control.Discovered in recent years, hospital environment surface, doctor
There are a variety of mycobacterium tuberculosis, Chang Yinqi hospital-acquired infections in treatment instrument and its water for cleaning.Therefore, it researches and develops safely and effectively
Disinfectant is turned off the effective means of spreading propagation.
Summary of the invention:
The object of the present invention is to provide a kind of disinfectants for efficiently killing mycobacterium tuberculosis, include levulic acid and dodecyl
The composition of benzene sulfonic acid sodium salt for the continuous disinfection of air, surface and skin, while also providing a kind of efficiently killing tuberculosis branch
The preparation method of the disinfectant of bacillus.
1 in order to overcome the drawbacks of the prior art, efficiently kills mycobacterium tuberculosis the purpose of the present invention is to provide a kind of
Disinfectant.
2 it is another object of the present invention to provide a kind of preparation sides of disinfectant for efficiently killing mycobacterium tuberculosis
Method.
3 third object of the present invention are to provide a kind of new use of disinfectant for efficiently killing mycobacterium tuberculosis
On the way.
4 to achieve the goals above, the invention provides the following technical scheme:
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 30% ~ 80%
The water of neopelex, weight percent 10% ~ 65% than 3% ~ 10%.
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 50%
The water of neopelex, weight percent 45% than 5%.PH value is set as 2 ~ 6.
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 80%
The water of neopelex, weight percent 12% than 8%.
A kind of efficient disinfectant for killing mycobacterium tuberculosis, further includes the coconut oleoyl amine third of weight percent 0.2% ~ 10%
The Tween 80 of base glycine betaine, weight percent 1% ~ 20%, pH value are set as 2 ~ 6.
A kind of efficient disinfectant for killing mycobacterium tuberculosis, further includes the hypromellose of weight percent 0.1% ~ 10%
Element, the glycerine of weight percent 1% ~ 20% or propylene glycol, pH value are set as 2 ~ 6.
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that and takes levulic acid
50g, neopelex 5g add water 45ml to make to dissolve, and obtain the disinfectant for efficiently killing mycobacterium tuberculosis.
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that
Step 1: taking the levulic acid of 30 ~ 80g, the neopelex of 3 ~ 10g, 0.2 ~ 10g cocamidopropyl propyl amide beet
Alkali, 1ml ~ 2ml Tween 80 add water 40ml ~ 60ml to make to dissolve；
Step 2: adding water and be diluted to 100ml, obtain disinfection solution.
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that
Step 1: taking the hydroxypropyl methylcellulose (HPMC) of 0.1~10g, with 5~10 mL glycerine or the wet dispersion of propylene glycol, gently
It stirs at homogeneous paste, adds 10~20mL purified water, stood 48 hours after stirring evenly, obtain system sufficiently swollen
It is swollen, and keep system fully transparent, without bubble, i.e. acquirement HPMC glue；Step 2: taking the levulic acid of 30 ~ 80g, 3 ~ 10g
Neopelex adds 20 ~ 65ml of water to make to be completely dissolved, obtains disinfection solution；
Step 3: disinfection solution full dose being added in HPMC glue, placement is fully transparent, and bubble-free is to get thimerosal.
A kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, is used for living body or non-live
Body is polluted by escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus, and especially mycobacterium tuberculosis pollutes.
A kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, is aqueous solution form, bubble
Foam agent and gel form, for killing big enteric bacterial pathogens, escherichia coli, mycobacterium tuberculosis and multi-drug resistant tuberculosis branch bar
Bacterium.
Another aspect of the present invention, the disinfectant of the efficient killing mycobacterium tuberculosis can be used for living body or non-live
Body disinfection.Preferably, the living body or non-living body be by one or more viruses, fungies and/or germ contamination, it is highly preferred that
Wherein the living body or non-living body be by big enteric bacterial pathogens, escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus,
Most preferably polluted by mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus.
Beneficial effect
1, in the present invention, the levulic acid is compounded with neopelex, is played in terms of sterilizing good
Synergistic effect, bactericidal effect have obtained the effect of " 1+1 > 2 ".The present invention efficiently kills the disinfectant pair of mycobacterium tuberculosis
Mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus have the characteristics that the killing time is short, killing effect is good.It is used alone simultaneously
Neopelex has irritation to human skin, and the application passes through Skin Irritation Test, the results showed that the present invention is high
Effect kills the disinfectant of mycobacterium tuberculosis to no skin irritation.
2, the present invention can be used for living body or non-living body by one or more viruses, fungi and germ contamination, wherein to big
Intestines cause a disease, escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus effect are preferable, pollute to mycobacterium tuberculosis
Effect is best.When action time is 1min, 40%LAV-4%SDBS sharply increases the killing rate presentation of three kinds of bacteriums.80%
LAV-8%SDBS is 99.99% or more to the killing rate of mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus effect 1min；Its
Middle 80%LAV-8%SDBS is 100.00% to the killing rate of mycobacterium tuberculosis effect 1min, efficiently kills mycobacterium tuberculosis,
It is short to kill the time；
3, the efficient disinfectant for killing mycobacterium tuberculosis provided by the invention is a kind of wide spectrum, kills that the time is short, kills and makees
It can be applied in cosmetics with stronger new disinfectant with good safety.The present invention has reached extension disinfection
The antibacterial time of agent, the effect for improving Disinfection Effect, while the present invention is non-stimulated to skin, the generation having no toxic side effect；Simultaneously
Levulic acid or neopelex are to eczema without effect, and the present invention is a kind of efficiently kills disappearing for mycobacterium tuberculosis
Toxic agent is to the efficient up to 60% of eczema.
Detailed description of the invention:
Attached drawing 1 is killing curve graph of the various concentration LAV+SDBS to escherichia coli
Attached drawing 2 is killing curve graph of the various concentration LAV+SDBS to mycobacterium tuberculosis
Attached drawing 3 is killing curve graph of the various concentration LAV+SDBS to substance of medicines-resistant branched tubercle bacillus
Attached drawing 4 be various concentration LAV-SDBS in 1min to the killing rate figure of escherichia coli
Attached drawing 5 be various concentration LAV-SDBS in 1min to the killing rate figure of mycobacterium tuberculosis
Specific embodiment:
Embodiment 1
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 30% ~ 80%
The water of neopelex, weight percent 10% ~ 65% than 3% ~ 10%.PH value is set as 2 ~ 6.
Preferably, the weight percent of levulic acid is 40% ~ 75%；The weight percent of neopelex is 4%
~8%；The weight percent of water is 10% ~ 56%, pH preferably 2 ~ 5, and optimal pH is 2.5.The disinfectant is aqueous solution form.
Embodiment 2
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent 5% including weight percent 50%
Neopelex, weight percent 45% water.
Or a kind of disinfectant for efficiently killing mycobacterium tuberculosis, levulic acid, weight hundred including weight percent 30%
Point than 5% neopelex, weight percent 65% water, pH 2.5.
Embodiment 3
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent 8% including weight percent 80%
Neopelex, weight percent 12% water.
Embodiment 4
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 30% ~ 80%
The water of neopelex, weight percent 10% ~ 65% than 3% ~ 10%；The coconut oleoyl amine of weight percent 0.2% ~ 10%
The Tween 80 of propyl betaine, weight percent 1% ~ 20%, pH value are set as 2 ~ 6.
Preferably, a kind of efficient disinfectant for killing mycobacterium tuberculosis, the levulinic including weight percent 40% ~ 80%
Acid, the neopelex of weight percent 4% ~ 10%, weight percent 0.2% ~ 10% Cocoamidopropyl betaine,
The water of the Tween 80 of weight percent 1% ~ 20%, weight percent 10% ~ 50%, pH value are set as 2 ~ 6.
It is optimal to be, a kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid including weight percent 50%,
5% neopelex, 5% Cocoamidopropyl betaine, the Tween 80 of weight percent 10%, 30% water, institute
The pH value for stating disinfectant is 2.5.The disinfectant of the efficient killing mycobacterium tuberculosis is foaming agent form.
Embodiment 5
A kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid, weight percent including weight percent 30% ~ 80%
The hydroxypropyl first of the water of neopelex, weight percent 10% ~ 65% than 3% ~ 10%, weight percent 0.1% ~ 10% is fine
Dimension element, the glycerine of weight percent 1% ~ 20% or propylene glycol, pH value are set as 2 ~ 6.
Preferably, a kind of efficient disinfectant for killing mycobacterium tuberculosis, the levulinic including weight percent 40% ~ 80%
Acid, the neopelex of weight percent 4% ~ 10%, the hydroxypropyl methylcellulose of weight percent 0.1% ~ 10%, weight hundred
Glycerine or propylene glycol point than 1% ~ 20%, 30% ~ 65% water, pH value is set as 2 ~ 6.
It is optimal to be, a kind of efficient disinfectant for killing mycobacterium tuberculosis, levulic acid including weight percent 80%,
The neopelex of weight percent 8%, the hydroxypropyl methylcellulose of weight percent 1%, weight percent 1% the third three
The water of alcohol, weight percent 10%, pH 2.5.The disinfectant is gel form.
Embodiment 6
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that the levulinic for taking 40 ~ 75g
The neopelex of acid, 4 ~ 8g, adds water 10ml ~ 45ml to make to dissolve, and obtains the disinfection for efficiently killing mycobacterium tuberculosis
Agent.
Or levulic acid 50g is taken, neopelex 5g adds water 45ml to make to dissolve, and obtains efficiently killing tuberculosis point
The disinfectant of branch bacillus.
Embodiment 7
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that
Step 1: taking the levulic acid of 30 ~ 80g, the neopelex of 3 ~ 10g, 0.2 ~ 10g cocamidopropyl propyl amide beet
Alkali, 1ml ~ 2ml Tween 80 add water 40ml ~ 60ml to make to dissolve；
Step 2: adding water and be diluted to 100ml, obtain the disinfectant for efficiently killing mycobacterium tuberculosis.
It is preferred that are as follows: a kind of preparation method for the disinfectant for efficiently killing mycobacterium tuberculosis comprises the following steps that
Step 1: taking the levulic acid of 50g, the neopelex of 5g, 10g Cocoamidopropyl betaine, 2ml tween
80, add water 40ml ~ 60ml to make to dissolve；
Step 2: adding water and be diluted to 100ml, obtain the disinfectant for efficiently killing mycobacterium tuberculosis.
Embodiment 8
A kind of preparation method of the efficient disinfectant for killing mycobacterium tuberculosis, comprises the following steps that
Step 1: taking the hydroxypropyl methylcellulose (HPMC) of 0.1~10g, with 5~10 mL glycerine or the wet dispersion of propylene glycol, gently
It stirs at homogeneous paste, adds 10~20mL purified water, stood 48 hours after stirring evenly, obtain system sufficiently swollen
It is swollen, and keep system fully transparent, without bubble, i.e. acquirement HPMC glue；Step 2: taking the levulic acid of 30 ~ 80g, 3 ~ 10g
Neopelex adds 20 ~ 65ml of water to make to be completely dissolved, obtains disinfection solution；
Step 3: disinfection solution full dose being added in HPMC glue, placement is fully transparent, and bubble-free kills tuberculosis to get efficient
The disinfectant of mycobacteria.
It is preferred that are as follows: a kind of preparation method for the disinfectant for efficiently killing mycobacterium tuberculosis comprises the following steps that
Step 1: taking the hydroxypropyl methylcellulose (HPMC) of 5g, with 8mL glycerine or the wet dispersion of propylene glycol, gently stir at uniformly paste
Shape adds 15mL purified water, through standing 48 hours after stirring evenly, expands system sufficiently, and keep system completely saturating
It is bright, without bubble, i.e. acquirement HPMC glue；Step 2: taking the levulic acid of 50g, the neopelex of 5g, add water
65ml makes to be completely dissolved, and obtains disinfection solution；
Step 3: disinfection solution full dose being added in HPMC glue, placement is fully transparent, and bubble-free kills tuberculosis to get efficient
The disinfectant of mycobacteria.
Embodiment 9
A kind of efficient disinfectant for killing mycobacterium tuberculosis, for living body or non-living body by escherichia coli, tuberculosis branch bar
Bacterium and substance of medicines-resistant branched tubercle bacillus pollution, especially mycobacterium tuberculosis pollute.
Embodiment 10
A kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, is aqueous solution form, foaming agent
And gel form, for killing big enteric bacterial pathogens, escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus.
Aqueous solution and foaming agent are used for air atomizing or spray application, and gel can be carried with tubule, and can be in trip
Way uses or uses in fluid distributor.
Embodiment 11
The present invention also provides a kind of methods of surface sterilization.Method includes the following steps: disinfectant of the invention is administered to
On surface or in atomizing air, the air borne of mycobacterium tuberculosis can be cut off, and disinfectant holding is made to be enough to kill
Viral, bacterium and/or fungi time.The application of above-mentioned disinfectant can by by above-mentioned disinfectant atomization, spraying and/or roller coating
To surface, or surface is immersed in disinfectant and is carried out.Surface to be sterilized can be living body surface, such as mammal is excellent
The skin for class of choosing, or be not have vital surface, in such as desk, medical apparatus or air or as described herein
Or any other surface known to user.
LAV Chinese meaning: levulic acid
LAV-SDBS Chinese meaning: levulic acid-neopelex
Disinfectant of the invention is prepared by commonsense method well known by persons skilled in the art, such as each component is total in any order
Mixed or mixing.The foaming agent and gelling agent used in above-mentioned preparation method are only to better understand this product example, the present invention
The foaming agent and gelling agent for including are not limited to the auxiliary material used in above-mentioned preparation method, should include other foaming commonly used in the art
Agent and gel auxiliary material.
Embodiment 12
Skin Irritation Test
The preparation of disinfectant: taking levulic acid 50g, neopelex 5g to add water 45ml, be stirred to dissolve, and mixing is equal
It is even, it stands, until the removal of foam is to get LAV-SDBS.Acquired solution is subjected to toxicology test.(LAV-SDBS Chinese contains
Justice: levulic acid-neopelex)
1, chmice acute Oral toxicity is tested
The disinfectant 0.5ml in above-described embodiment 1 is taken, intragastric administration on mice, LD are given0=900mg/kg, LD50=6280mg/kg, LD100
=2694mg/kg.Sentence by acute oral toxicity test result judgment criteria in Ministry of Public Health's " disinfection technology standard " (version in 2002)
Fixed, this product belongs to nontoxic rank.
2, Skin Irritation Test
The sample in above-described embodiment 1 is taken, carries out an intact skin irritant test with rabbit, the results showed that this product is to family's rabbit
Skin stimulus index is 0, and stimulus intensity is nonirritant.Concrete outcome is as follows:
Embodiment 13
The preparation of disinfectant: taking levulic acid 50g, neopelex 5g to add water 45ml, be stirred to dissolve to get
Resulting solution is used for following biological test by LAV-SDBS.
(LAV-SDBS Chinese meaning: levulic acid-neopelex)
1, bacteriostatic test is through extracorporeal bacteria inhibitor test, and formula provided by the invention is to escherichia coli, Candida albicans and tuberculosis point
Branch bacillus has good bacteriostasis, the most obvious to the effect of mycobacterium tuberculosis；LAV and SDBS is used in mixed way relatively individually
Using LAV to the antibacterial more preferable of escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus.
This product can effectively kill simultaneously Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and Candida albicans,
Mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus.
Bactericidal effect of the 50%LVA-5%SDBS to different bacterium
2, killing effect (LAV-SDBS Chinese meaning: levulic acid-dodecyl benzene sulfonic acid of the LAV-SDBS to different bacterium
Sodium)
Substance of medicines-resistant branched tubercle bacillus: while to rifampin and Isoniazid-resistant.
The LVA-SDBS of various concentration is respectively acting on escherichia coli, mycobacterium tuberculosis and multi-drug resistant tuberculosis branch bar
The bacterium different time, its killing effect is observed, as a result see the table below.The result shows that with the increasing addition of LAV-SDBS concentration
The extension of time obviously rises the killing rate of three kinds of bacteriums, and when action time is 1min, 40%LAV-4%SDBS is thin to three kinds
The killing rate presentation of bacterium sharply increases.80%LAV-8%SDBS acts on mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus
The killing rate of 1min is 99.99% or more；Wherein 80%LAV-8%SDBS is to the killing rate of mycobacterium tuberculosis effect 1min
100.00%, efficiently kill mycobacterium tuberculosis；
40%LAV-4%SDBS to mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus effect 1min killing rate be 97% with
On；Killing of the 20%LAV-2%SDBS to escherichia coli and mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus effect 30min
Rate can be only achieved 99% or more.It is with a wide range of applications.
Killing effect (n=6) of the various concentration LAV-SDBS to bacterium
3, therapeutic effect of the LAV-SDBS to eczema
Sample preparation: taking levulic acid 50g, neopelex 5g to add water 45ml, be stirred to dissolve, mix to get.
Levulic acid control group: taking levulic acid 50g, adds water 50ml, is stirred to dissolve, mix to get.
Neopelex control group: taking neopelex 5g, adds water 95ml, is stirred to dissolve, and mixes,
To obtain the final product.
Illustrate beneficial effects of the present invention below by way of the mode of test example.
(1) patient criteria
Patient 60, male 30, female 30.The course of disease -2 months 1 week.
(2) diagnostic criteria
Erythema, papule, blister or the erosion that primary symptom: cheek, forehead, glabella, scalp or body other position gatherings or is dispersed in, companion
Itch, skin lesion form of diverse, distribution are mostly symmetrical.
(3) standard is treated
Syndrome Scale (quantizating index) is light: only a small amount of light red patch, with a small amount of papule, phlysis and small rotten to the corn, infiltration
Liquid；Patient occasionally scratches skin, does not influence to sleep.In: papule, blister, erosion based on, partially with the scales of skin that peel off；It is more to scratch number,
Sleep is fine.Weight: erythema is bright-coloured, partially can be because of skin ulceration secondary infection based on rotten to the corn, sepage；Scratching is obvious, scratching time
Number is more, influences to sleep.
Cardinal symptom sign: observation skin color, erosive exudating area, patient scratch situation, by without, it is light, in, be again layered
Standard counts 0,2,4,6 point respectively.Total mark × 100% before disease integration rate=(total mark after pre-treatment score-treatment)/treatment.
Criterion of therapeutical effect is referring to " Clinical Researches of New Drugs guideline "
Clinical recovery: clinical symptoms, sign disappear or mostly disappear, disease integration rate >=95%；
Effective: clinical symptoms, sign are obviously improved, 70%≤disease integration rate≤95%；
Effective: clinical symptoms, sign take a favorable turn, 30%≤disease integration rate≤70%；
Invalid: clinical symptoms, sign are not improved, disease integration rate≤30%；
(4) treatment method
A: medication: after diagnosis is clear, treatment group patient is all made of drug smearing and treats；Morning, noon, night respectively apply
Smear primary, 1 day smearing 3 times.
B: no medication taboo.
(5) treatment results
Treatment time: continuous treatment 7 days
Efficacy determination: observing the improvement situation of patient clinical symptom and sign after treatment, whether there is or not recurrences to aggravate for the state of an illness in 1 week, has
It has no adverse reaction.
Concrete outcome see the table below.
Therapeutic effect of the different samples to eczema
| Group | Case load | Recovery from illness | Significantly | Effectively | In vain | It is efficient |
| Levulic acid group | 20 | 0 | 0 | 0 | 20 | 0% |
| Neopelex group | 20 | 0 | 0 | 0 | 20 | 0% |
| Levulic acid and neopelex joint group | 20 | 2 | 3 | 7 | 8 | 60% |
Above-mentioned test illustrate levulic acid group and neopelex group to eczema without effect, and levulic acid of the present invention and
Neopelex joint group is to the efficient up to 60% of eczema.
It should be understood by those ordinary skilled in the art that: the discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under thinking of the invention, the above implementation
It can also be combined between technical characteristic in example or different embodiments, step can be realized with random order, and be existed
Many other variations of different aspect present invention as described above, for simplicity, they are not provided in details.Therefore, all
Within the spirit and principles in the present invention, any omission, modification, equivalent replacement, improvement for being made etc., should be included in the present invention
Protection scope within.
Claims (10)
1. a kind of disinfectant for efficiently killing mycobacterium tuberculosis, which is characterized in that the acetyl including weight percent 30% ~ 80%
Propionic acid, the neopelex of weight percent 3% ~ 10%, weight percent 10% ~ 65% water.
2. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that including weight
Measure the water of the levulic acid of percentage 50%, the neopelex of weight percent 5%, weight percent 45%.
3. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that including weight
Measure the water of the levulic acid of percentage 80%, the neopelex of weight percent 8%, weight percent 12%.
4. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that further include
The Tween 80 of the Cocoamidopropyl betaine of weight percent 0.2% ~ 10%, weight percent 1% ~ 20%, pH value is set as 2 ~
6。
5. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that further include
The glycerine or propylene glycol of the hydroxypropyl methylcellulose of weight percent 0.1% ~ 10%, weight percent 1% ~ 20%, pH value are set as 2
~6。
6. a kind of preparation method for the disinfectant for efficiently killing mycobacterium tuberculosis, which is characterized in that comprise the following steps that and take second
Acyl propionic acid 50g, neopelex 5g add water 45ml to make to dissolve, and obtain the disinfectant for efficiently killing mycobacterium tuberculosis.
7. a kind of preparation method for the disinfectant for efficiently killing mycobacterium tuberculosis, which is characterized in that comprise the following steps that
Step 1: taking the levulic acid of 30 ~ 80g, the neopelex of 3 ~ 10g, 0.2 ~ 10g cocamidopropyl propyl amide beet
Alkali, 1ml ~ 2ml Tween 80 add water 40ml ~ 60ml to make to dissolve；
Step 2: adding water and be diluted to 100ml, obtain disinfection solution.
8. a kind of preparation method for the disinfectant for efficiently killing mycobacterium tuberculosis, which is characterized in that comprise the following steps that
Step 1: taking the hydroxypropyl methylcellulose (HPMC) of 0.1~10g, with 5~10 mL glycerine or the wet dispersion of propylene glycol, gently
It stirs at homogeneous paste, adds 10~20mL purified water, stood 48 hours after stirring evenly, obtain system sufficiently swollen
It is swollen, and keep system fully transparent, without bubble, i.e. acquirement HPMC glue；Step 2: taking the levulic acid of 30 ~ 80g, 3 ~ 10g
Neopelex adds 20 ~ 65ml of water to make to be completely dissolved, obtains disinfection solution；
Step 3: disinfection solution full dose being added in HPMC glue, placement is fully transparent, and bubble-free is to get thimerosal.
9. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that for living
Body or non-living body are polluted by escherichia coli, mycobacterium tuberculosis and substance of medicines-resistant branched tubercle bacillus, especially tuberculosis branch bar
Bacterium pollution.
10. a kind of a kind of disinfectant for efficiently killing mycobacterium tuberculosis as described in claim 1, which is characterized in that be water
Solution form, foaming agent and gel form, for killing big enteric bacterial pathogens, escherichia coli, mycobacterium tuberculosis and multi-drug resistant
Mycobacterium tuberculosis.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633882A (en) * | 2018-04-26 | 2018-10-12 | 首都医科大学附属北京胸科医院 | A kind of mycobacterium tuberculosis disinfectant |
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2019
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633882A (en) * | 2018-04-26 | 2018-10-12 | 首都医科大学附属北京胸科医院 | A kind of mycobacterium tuberculosis disinfectant |
Non-Patent Citations (4)
| Title |
|---|
| 吕霞丽等: "乙酰丙酸-十二烷基硫酸钠对大肠埃希菌、耻垢分枝杆菌、结核分枝杆菌的杀灭作用研究", 《北京医学》 * |
| 吕霞丽等: "乙酰丙酸－十二烷基硫酸钠对结核分枝杆菌的杀灭作用及其安全性毒理学评价", 《首都医科大学学报》 * |
| 吕霞丽等: "乙酰丙酸-十二烷基硫酸钠对结核分枝杆菌的杀灭作用及其杀菌机制的蛋白组学研究", 《中国热带医学》 * |
| 杨修军等: "含氯复方消毒剂杀灭结核杆菌效果的试验观察", 《中国消毒学杂质》 * |
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Application publication date: 20190517 |