CN109735461A - One lactobacillus plantarum and its application in reduction fish tea Content of Biogenic Amines - Google Patents
One lactobacillus plantarum and its application in reduction fish tea Content of Biogenic Amines Download PDFInfo
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Abstract
The invention discloses a lactobacillus plantarum (Lactobacillus plantarum) Yc-2, deposit number CGMCC NO.16614；The method for reducing fish tea Content of Biogenic Amines using the lactobacillus plantarum Yc-2, comprising steps of being 10 by concentration6~108The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is seeded in fermentation system by the 1%~4% of raw material fish weight, ferments as leavening.By inoculated plant lactobacillus Yc-2, the biogenic amine in fish tea system is inhibited well, and the degradation rate of Yu Chazhong biogenic amine total content especially can achieve 91% or more to the degradation rate of histamine up to 95% or more.
Description
Technical field
The present invention relates to microbe leaven technical fields, and in particular to one plant of lactobacillus plantarum for capableing of degradation biological amine
And its reducing the application in fish tea Content of Biogenic Amines.
Background technique
Fish tea, also referred to as fish acid are a kind of universal homemade flavor fermentation food of Li Miao clansman.Fish tea is usually by fresh fish
Class and ripe carbohydrate are made, and after microbial fermentation, the taste concentrated acid of formation, fish full of nutrition, stay in grade add
Work product.Micropopulation is complicated in natural fermentation process, and part of microbes catalysis free amine group acid decarboxylation generates
The biogenic amine being harmful to the human body brings security risk to this based food.A small amount of biogenic amine intake will not influence human health, but
When the biogenic amine of human body external source excess intake, it can cause to have a headache, blood pressure increases, nervous system and cardiovascular system injury, abdomen
The severe reactions such as rush down and vomit.Histamine is that biogenic amine Poisoning is maximum, and tyrasamine takes second place；Itself of putrescine and cadaverine toxicity compared with
It is small, but the toxicity of histamine and tyrasamine can be enhanced.U.S. FDA provides that the histamine content in aquatic products must not exceed 50mg/kg, and
It is recommended that tyramine content does not exceed 100mg/kg, biogenic amine total amount does not exceed 1000mg/kg.Biogenic amine thermal stability is good, and one
Denier generates, it is difficult to remove during the cooking process；Therefore the accumulation of control fermentation process biogenic amine is food-safe extremely important.
Due to the particularity of food production process, it is difficult the physical parameter by changing production process generally to realize to life
The control of object amine；And product is handled by physics or the method for adding chemical substance, then it may result in food
Nutrient loss, flavor change or even safety leaves a question open.Reasonably select leavening, especially have degradation biological amine ability,
There is no the microorganism of security risk, the biogenic amine accumulation in food can be effectively controlled.
Lactic acid bacteria is that one kind can make carbohydrate fermentation and generate the general designation of the bacterium of lactic acid, is important in food industry
Leavening.Lactic acid bacteria has the advantages that not damage food nutrition, promotes product special flavour；There is bacteriostasis simultaneously, can make
Environmental acidification, secretion antibacterial material, and then effectively inhibit some pathogens and spoilage organisms.In addition, most lactic acid bacterias are safe, strong
Health, nonhazardous effect.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for reducing biogenic amine in fish tea product using lactobacillus plantarum.
The present invention is achieved by the following technical solutions:
In order to achieve the above objectives, the present invention provides a lactobacillus plantarum (Lactobacillus plantarum) Yc-
2, deposit number CGMCC NO.16614, are preserved in that " China Committee for Culture Collection of Microorganisms is general on October 24th, 2018
Logical microorganism " center ", abbreviation CGMCC, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences microorganism is ground
Study carefully institute, postcode: 10010.Above-mentioned lactobacillus plantarum is isolated from fermented food, carries out 16S using bacterial universal primers 27F/1492R
RDNA Sequence Identification is lactobacillus plantarum.
Invention also provides a kind of methods for reducing fish tea Content of Biogenic Amines using the lactobacillus plantarum, including step
It is rapid: to be 10 by concentration6~108The lactobacillus plantarum bacterium solution of CFU/mL is as leavening, by 1%~4% inoculation of raw material fish weight
To fermentation system, fermentation is synchronized；Wherein, it is CGMCC that the lactobacillus plantarum, which is lactobacillus plantarum Yc-2 deposit number,
NO.16614。
Under preferred embodiment, the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum, comprising steps of
S1, pretreatment of raw material: taking the raw material flesh of fish, flitch, be placed in saturated salt solution, 4~15 DEG C of 1~3h of immersion,
It is drained and standby；
S2, production fish tea: by fish block, rice and 10 obtained by step S16~108CFU/mL lactobacillus plantarum Yc-2 bacterium solution is mixed
It closes uniformly, is placed in container, 20~25 DEG C of sealings are placed 32~37 days, and fish tea is obtained；The lactobacillus plantarum Yc-2 deposit number is
CGMCC NO.16614；
Wherein, the addition weight ratio of the fish block, rice and lactobacillus plantarum Yc-2 bacterium solution is 1:(1~4): (0.01~
0.04)。
Under preferred embodiment, fish block size described in step S1 are as follows: 2cm × 2cm × 2cm；The raw material fish be grass carp, carp or
Bighead.
It under preferred embodiment, is used after 121 DEG C of sterilizing 20min of container described in step S2, the rice is rice and water by weight
Amount is mixed than 1:1.5, obtained by 100 DEG C of heating 30min；The lactobacillus plantarum Yc-2 bacterium solution the preparation method comprises the following steps: by plant cream
Bacillus Yc-2 in the flat lining out of MRS agar medium, 37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded to MRS meat
In soup culture medium, 37 DEG C, oxygen consumption, stationary culture 12h；Thalline were collected by centrifugation, with the normal saline dilution of mass fraction 0.85%
To 106~108CFU/mL；
Wherein, the MRS agar medium ingredient are as follows: peptone 10.0g/L, powdered beef 8.0g/L, yeast powder 4.0g/L,
Glucose 20.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, sodium acetate 5.0g/L, magnesium sulfate 0.2g/L,
Manganese sulfate 0.04g/L, agar 14.0g/L, Tween 80 1.0g/L, 25 DEG C of pH value is 6.5 ± 0.2；
The MRS broth bouillon ingredient are as follows: peptone 10.0g/L, beef extract powder 8.0g/L, yeast extract 4.0g/L,
Glucose 20.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, sodium acetate 5g/L, magnesium sulfate 0.2g/L, sulphur
Sour manganese 0.04g/L, Tween 80 1.0g/L, 5 DEG C of pH value is 5.7 ± 0.2.
Under preferred embodiment, the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum, comprising steps of
S1, pretreatment of raw material:
Grass carp removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；Flesh of fish block
4 DEG C of immersion 2h are placed in saturated salt solution, it is drained and standby；
S2, production fish tea:
After cleaning container, 121 DEG C of high-temperature sterilization 20min；Take fish block and 0.6g obtained by 240g rice, 60g step S1
108The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 35 days is placed in 20 DEG C of sealings, is obtained
Fish tea；
Wherein, it is 1:1.5, rice obtained by 100 DEG C of heating 30min that the rice, which is the weight ratio of rice and water,；The plant
Object lactobacillus Yc-2 bacterium solution the preparation method comprises the following steps: by lactobacillus plantarum Yc-2 in the flat lining out of MRS agar medium, 37 DEG C,
For 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon for oxygen consumption, stationary culture, 37 DEG C, oxygen consumption, stationary culture 12h；
10000r/min centrifugation 10min after collect thallus, by thallus with mass fraction be 0.85% aqueous solution of sodium chloride dilution, be prepared into
108The bacteria suspension of CFU/mL.
The beneficial effects of the present invention are:
Lactobacillus plantarum Yc-2 provided by the invention can be used for reducing the biology in fermented fish products especially fish tea product
Amine content has very extensive application prospect.
Specific embodiment
Below by specific implementation example, the present invention will be further described.
The present invention provides a lactobacillus plantarum (Lactobacillus plantarum) Yc-2, deposit number CGMCC
NO.16614 is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on October 24th, 2018,
Abbreviation CGMCC, address are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode:
10010。
Above-mentioned lactobacillus plantarum is isolated from fermented food, carries out 16SrDNA sequence using bacterial universal primers 27F/1492R
Identification is lactobacillus plantarum.
The method for preserving of the lactobacillus plantarum: well-grown single colonie is selected from MRS agar medium plate and is connect
Kind to 3mL MRS broth bouillon, 37 DEG C, oxygen consumption, stationary culture for 24 hours, take 1mL culture solution to be added to containing 30% glycerol of 1mL
Cryopreservation tube in, in -80 DEG C of Storage in refrigerator.
The cultural method of the lactobacillus plantarum: by the lactobacillus plantarum of preservation in the flat lining out of MRS agar medium,
37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded in MRS broth bouillon, 37 DEG C, oxygen consumption, stationary culture 12h.
The MRS agar medium ingredient are as follows: peptone 10.0g/L, powdered beef 8.0g/L, yeast powder 4.0g/L, grape
Sugared 20.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, sodium acetate 5.0g/L, magnesium sulfate 0.2g/L, sulfuric acid
Manganese 0.04g/L, agar 14.0g/L, Tween 80 1.0g/L, 25 DEG C of pH value is 6.5 ± 0.2.
The MRS broth bouillon ingredient are as follows: peptone 10.0g/L, beef extract powder 8.0g/L, yeast extract 4.0g/L,
Glucose 20.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, sodium acetate 5g/L, magnesium sulfate 0.2g/L, sulphur
Sour manganese 0.04g/L, Tween 80 1.0g/L, 25 DEG C of pH value is 5.7 ± 0.2.
Embodiment 1:
The method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum Yc-2 (CGMCC No.16614), comprising steps of
S1, pretreatment of raw material:
Fresh grass carp removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；
Flesh of fish block is placed in saturated salt solution 4 DEG C of immersion 2h, drained and standby；
Rice is through over cleaning, and the weight ratio of the rice and water is 1:1.5, and 100 DEG C of heating 30min obtain rice, cool standby
With；
S2, production fish tea:
Cleaning container is clean, 121 DEG C of high-temperature sterilization 20min；Take fish block and 0.6g obtained by 240g rice, 60g step S1
108The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 35 days is placed in 20 DEG C of sealings, is obtained
Fish tea；
Wherein, the lactobacillus plantarum Yc-2 bacterium solution the preparation method comprises the following steps: by lactobacillus plantarum Yc-2 in MRS agar culture
The flat lining out of base, 37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon, 37 DEG C,
Oxygen consumption, stationary culture 12h；Thallus is collected after 10000r/min centrifugation 10min, by thallus sterile saline (sodium chloride
0.85% aqueous solution, w/v, g/mL) it is prepared into 108The bacteria suspension of CFU/mL.
Take fish tea obtained by the present embodiment, by dansyl Cl column front derivation-high performance liquid chromatography detect histamine in the flesh of fish,
The content of tyrasamine, putrescine, cadaverine, tryptamines and phenyl ethylamine, testing result are as shown in table 1.
Embodiment 2:
The method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum Yc-2 (CGMCC No.16614), comprising steps of
S1, pretreatment of raw material:
Fresh carp removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；
Flesh of fish block is placed in saturated salt solution 10 DEG C of immersion 3h, drained and standby；
Rice is through over cleaning, and the weight ratio of the rice and water is 1:1.5, and 100 DEG C of heating 30min obtain rice, cool standby
With；
S2, production fish tea:
Cleaning container is clean, 121 DEG C of high-temperature sterilization 20min；Take fish block and 2.4g obtained by 120g rice, 120g step S1
107The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 37 days is placed in 25 DEG C of sealings, is obtained
Fish tea；
Wherein, the lactobacillus plantarum Yc-2 bacterium solution the preparation method comprises the following steps: by lactobacillus plantarum Yc-2 in MRS agar culture
The flat lining out of base, 37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon, 37 DEG C,
Oxygen consumption, stationary culture 12h；Thallus is collected after 10000r/min centrifugation 10min, by thallus 2mL sterile saline (sodium chloride
0.85% aqueous solution, w/v, g/mL) it is prepared into 107The bacterium solution of CFU/mL.
Take fish tea obtained by the present embodiment, by dansyl Cl column front derivation-high performance liquid chromatography detect histamine in the flesh of fish,
The content of tyrasamine, putrescine, cadaverine, tryptamines and phenyl ethylamine, testing result are as shown in table 1.
Embodiment 3:
The method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum Yc-2 (CGMCC No.16614), comprising steps of
S1, pretreatment of raw material:
Fresh bighead removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；
Flesh of fish block is placed in saturated salt solution 15 DEG C of immersion 1h, drained and standby；
Rice is through over cleaning, and the weight ratio of the rice and water is 1:1.5, and 100 DEG C of heating 30min obtain rice, cool standby
With；
S2, production fish tea:
Cleaning container is clean, 121 DEG C of high-temperature sterilization 20min；Take fish block and 4g obtained by 200g rice, 100g step S1
106The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 32 days is placed in 22 DEG C of sealings, is obtained
Fish tea；
The lactobacillus plantarum Yc-2 bacterium solution the preparation method comprises the following steps: lactobacillus plantarum Yc-2 is put down in MRS agar medium
Lining out, 37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon, 37 DEG C, oxygen consumption,
Stationary culture 12h；Thallus is collected after 10000r/min centrifugation 10min, by thallus 2mL sterile saline (sodium chloride
0.85% aqueous solution, w/v, g/mL) it is prepared into 106The bacterium solution of CFU/mL.
Take fish tea obtained by the present embodiment, by dansyl Cl column front derivation-high performance liquid chromatography detect histamine in the flesh of fish,
The content of tyrasamine, putrescine, cadaverine, tryptamines and phenyl ethylamine, testing result are as shown in table 1.
Comparative example 1
Spontaneous fermentation prepares fish tea product, comprising steps of
S1, pretreatment of raw material:
Fresh grass carp removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；
Flesh of fish block is placed in saturated salt solution 4 DEG C of immersion 2h, drained and standby；
Rice is through over cleaning, and the weight ratio of the rice and water is 1:1.5, and 100 DEG C of heating 30min obtain rice, cool standby
With；
S2, production fish tea:
Cleaning container is clean, 121 DEG C of high-temperature sterilization 20min；Take fish block mixing obtained by 240g rice and 60g step S1 equal
It is even, it is placed in the container, fermentation 35 days is placed in 22 DEG C of sealings, obtains fish tea.
Take fish tea obtained by this comparative example, by dansyl Cl column front derivation-high performance liquid chromatography detect histamine in the flesh of fish,
The content of tyrasamine, putrescine, cadaverine, tryptamines and phenyl ethylamine, testing result are as shown in table 1.
Comparative example 2
The method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum PL-2 (CGMCC No.13919), comprising steps of
Wherein, the lactobacillus plantarum PL-2, is isolated from pickles, is purchased from " Chinese microorganism strain preservation conservator
Meeting common micro-organisms center ", deposit number are as follows: CGMCC 13919；
S1, pretreatment of raw material:
Fresh grass carp removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm fritter；
Flesh of fish block is placed in saturated salt solution 4 DEG C of immersion 2h, drained and standby；
Rice is through over cleaning, and the weight ratio of the rice and water is 1:1.5, and 100 DEG C of heating 30min obtain rice, cool standby
With；
S2, production fish tea:
Cleaning container is clean, 121 DEG C of high-temperature sterilization 20min；Take fish block and 0.6g obtained by 240g rice, 60g step S1
108The lactobacillus plantarum PL-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 35 days is placed in 20 DEG C of sealings, is obtained
Fish tea；
Wherein, the lactobacillus plantarum PL-2 bacterium solution the preparation method comprises the following steps: by lactobacillus plantarum PL-2 in MRS agar culture
The flat lining out of base, 37 DEG C, oxygen consumption, stationary culture for 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon, 37 DEG C,
Oxygen consumption, stationary culture 12h；Thallus is collected after 10000r/min centrifugation 10min, by thallus 2mL sterile saline (sodium chloride
0.85% aqueous solution, w/v, g/mL) it is prepared into 108The bacteria suspension of CFU/mL.
Take fish tea obtained by this comparative example, by dansyl Cl column front derivation-high performance liquid chromatography detect histamine in the flesh of fish,
The content of tyrasamine, putrescine, cadaverine, tryptamines and phenyl ethylamine, testing result are as shown in table 1.
Influence of the lactobacillus plantarum Yc-2 (CGMCC No.16614) to Content of Biogenic Amines in fish tea is as shown in table 1:
Influence of the 1 lactobacillus plantarum Yc-2 of table (CGMCC No.16614) to Content of Biogenic Amines in fish tea
The experimental results showed that the biology by inoculated plant lactobacillus Yc-2 (CGMCC No.16614), in fish tea system
Amine is inhibited well, in embodiment 1,2,3 degradation rate of six kinds of biogenic amine total contents be respectively 96.94%, 96.15%,
95.66%, especially reaching respectively to the degradation rate of histamine is 95.25%, 94.09%, 91.23%；Compared to comparative example 2, plant
Object lactobacillus Yc-2 (CGMCC No.16614) is more preferable to the degradation efficiency of biogenic amine, therefore the bacterial strain can be used as low biogenic amine fish
The leavening of tea product.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art within the technical scope of the present disclosure, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (9)
1. a lactobacillus plantarum (Lactobacillus plantarum) Yc-2, has been preserved in " Chinese microorganism strain preservation
Administration committee's common micro-organisms center ", abbreviation CGMCC, deposit number CGMCC NO.16614.
2. a kind of method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum, which is characterized in that comprising steps of being by concentration
106~108The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is seeded to fermentation as leavening, by the 1%~4% of raw material fish weight
In system, ferment；Wherein, the lactobacillus plantarum Yc-2 deposit number is CGMCC NO.16614.
3. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 2, which is characterized in that including
Step:
S1, pretreatment of raw material: taking the raw material flesh of fish, flitch, be placed in saturated salt solution, and 4~15 DEG C of 1~3h of immersion are drained
It is spare；
S2, production fish tea: by fish block, rice and 10 obtained by step S16~108The mixing of CFU/mL lactobacillus plantarum Yc-2 bacterium solution, sets
In in container, 20~25 DEG C of sealings are placed 32~37 days, obtain fish tea；The lactobacillus plantarum Yc-2 deposit number is CGMCC
NO.16614；
Wherein, the addition weight ratio of the fish block, rice and lactobacillus plantarum Yc-2 bacterium solution is 1:(1~4): (0.01~
0.04)。
4. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that step
Fish block size described in S1 are as follows: 2cm × 2cm × 2cm.
5. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that step
Raw material fish described in S1 is grass carp, carp or bighead.
6. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that step
It is used after 121 DEG C of sterilizing 20min of container described in S2.
7. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that step
Rice described in S2 is that rice is mixed with water by weight 1:1.5, obtained by 100 DEG C of heating 30min.
8. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that step
Lactobacillus plantarum Yc-2 bacterium solution described in S2 the preparation method comprises the following steps: by lactobacillus plantarum Yc-2 on MRS agar medium plate draw
Line, 37 DEG C, oxygen consumption, stationary culture for 24 hours；Picking single colonie is seeded in MRS broth bouillon, 37 DEG C, oxygen consumption, stationary culture
12h；Thalline were collected by centrifugation, with the normal saline dilution of mass fraction 0.85% to 106~108CFU/mL。
9. the method for reducing fish tea Content of Biogenic Amines using lactobacillus plantarum according to claim 3, which is characterized in that including
Step:
S1, pretreatment of raw material: taking grass carp, removes head, tail portion, fishbone and internal organ, the flesh of fish is cut into 2cm × 2cm × 2cm
Fritter；Fish block is placed in saturated salt solution 4 DEG C of immersion 2h, drained and standby；
S2, production fish tea: by cleaning container, 121 DEG C of high-temperature sterilization 20min；Take fish block obtained by 240g rice, 60g step S1 and
0.6g 108The lactobacillus plantarum Yc-2 bacterium solution of CFU/mL is uniformly mixed, and is placed in the container, and fermentation 35 is placed in 20 DEG C of sealings
It, obtains fish tea；
Wherein, it is 1:1.5, rice obtained by 100 DEG C of heating 30min that the rice, which is the weight ratio of rice and water,；The plant cream
Bacillus Yc-2 bacterium solution the preparation method comprises the following steps: by lactobacillus plantarum Yc-2 in the flat lining out of MRS agar medium, 37 DEG C, oxygen consumption,
For 24 hours, picking single colonie is seeded in 3ml MRS broth bouillon stationary culture, 37 DEG C, oxygen consumption, stationary culture 12h；10000r/
Thallus is collected after min centrifugation 10min, the sodium-chloride water solution of thallus mass fraction 0.85% is diluted, is prepared into 108CFU/
The bacteria suspension of mL.
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| CN111254101A (en) * | 2020-04-27 | 2020-06-09 | 中粮营养健康研究院有限公司 | Lactobacillus plantarum and microbial inoculum and application thereof in biogenic amine degradation and yellow wine production |
| CN111454858A (en) * | 2020-04-03 | 2020-07-28 | 大连工业大学 | Method for inhibiting biogenic amine accumulation of amine-producing bacteria by using hurdle technology |
| CN111789205A (en) * | 2020-07-29 | 2020-10-20 | 大连工业大学 | Preparation method of fermented agaric whey protein isolate composite beverage |
| CN112931796B (en) * | 2021-02-03 | 2023-09-01 | 大连工业大学 | Ester-producing lactic acid bacteria and application thereof in sour meat production |
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| CN110499271A (en) * | 2019-09-02 | 2019-11-26 | 千禾味业食品股份有限公司 | A kind of lactobacillus plantarum QR19 and its application |
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| CN111254101B (en) * | 2020-04-27 | 2020-07-28 | 中粮营养健康研究院有限公司 | Lactobacillus plantarum and microbial inoculum and application thereof in biogenic amine degradation and yellow wine production |
| CN111789205A (en) * | 2020-07-29 | 2020-10-20 | 大连工业大学 | Preparation method of fermented agaric whey protein isolate composite beverage |
| CN112931796B (en) * | 2021-02-03 | 2023-09-01 | 大连工业大学 | Ester-producing lactic acid bacteria and application thereof in sour meat production |
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