CN101701203B - Lactobacillus plantarum and application thereof - Google Patents
Lactobacillus plantarum and application thereof Download PDFInfo
- Publication number
- CN101701203B CN101701203B CN200910232713XA CN200910232713A CN101701203B CN 101701203 B CN101701203 B CN 101701203B CN 200910232713X A CN200910232713X A CN 200910232713XA CN 200910232713 A CN200910232713 A CN 200910232713A CN 101701203 B CN101701203 B CN 101701203B
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- bacterial strain
- biogenic amine
- amine
- starter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a lactobacillus plantarum bacterial strain with the collection number of CGMCC No.3167. The bacterial strain can be gathered in an improved MRS liquid culture medium, is white on the MRS agar culture medium, straight, short and Gram-negative, is free from flagellum, is in homofermentation, produces no gas when fermenting glucose, produces acetic acid and lactic acid when fermenting ribose and does not reduce nitrite. When being applied, the bacterial strain is activated and performed with culture expansion and thallus collection to form a leaven, which biogenic amine oxidase is generated in fermenting and producing meat products to inhibit amine producing bacteria from growing as well as oxidize and degrade biogenic amine. The lactobacillus plantarum has the advantages of strong growth capability and simple leaven preparation technology; the leaven can effectively inhibit enterobacter from growing and breeding when fermenting and producing meat products so as to control the generation of the biogenic amine; in addition, biogenic amine oxidase produced by the lactobacillus plantarum of the invention can oxidize and degrade cadaverine and putrescine; thus, when serving as a leaven to produce and ferment meat products, the lactobacillus plantarum can effectively reduce accumulation of biogenic amine.
Description
Technical field
Good milk-acid bacteria of the present invention's one strain and application thereof belong to biological technical field.
Background technology
Biogenic amine is one type of nitrogenous aliphatics, aromatic series or heterocyclic organic cpds.Mainly contain tyrasamine, histamine, putrescine, cadaverine, phenylethylamine, tryptamines, spermine and spermidine.Will produce murder by poisoning when in human body, running up to comparatively high amts, as: outside blood vessel expands and causes hypertension and headache, and the contraction of enteron aisle unstriated muscle causes abdominal cramps, diarrhoea and vomiting etc., the serious entail dangers to life of going back.
In the food in common 8 kinds of biogenic amines spermine and spermidine be to bring in the raw material, other biogenic amine produces by the amino acid decarboxylase catalytic amino acid decarboxylation of microorganisms, it mainly is present in the high leavened food of protein contnt.Biogenic amine content has received vast concern in the fermented meat prods, and the foreign scholar has done a large amount of investigation, thinks biogenic amine ubiquity in fermented meat prods, and particularly in fermented sausages, biogenic amine is considerably beyond the FDA specified standards in some products.For example, the traditional Chinese sausage belongs to the spontaneous fermentation sausage, and wherein mikrobe fungus strain and complicacy thereof are found in the investigation of this seminar, biogenic amine content in the traditional Chinese sausage, and high far away foreign scholar's report, biogenic amine content reaches 1651.84mg/kg.Health to the human consumer has material impact.The main body biogenic amine is generally tyrasamine, putrescine and cadaverine in the fermented meat prods.
Control to biogenic amine mainly can realize aspect two in traditional meat product fermentative prodn at present: the one, and the growth of amine bacterium is produced in control, adopts the biogenic amine oxydase of the microorganisms that can produce the thing amine oxidase on the other hand, reduces the biogenic amine accumulation.For example, product amine bacterium main in the traditional Chinese sausage is an enterobacteria, and it is in the logarithmic growth later stage that enterobacteria produces biogenic amine, and bacterium number just collaborative biogenic amine that produces when reaching finite concentration.So suppress the growth of enterobacteria, be controlled at minimum concentration to enterobacteria, be effectively to control the important measures that biogenic amine produces.
Therefore; Generation and accumulation for biogenic amine in the control traditional Chinese sausage; Strong with energy for growth, it is strong to suppress the enterobacteria ability, and producing the thing amine oxidase is purpose; From the low traditional Chinese sausage sample of biogenic amine content, separate a lactobacillus plantarum, can effectively control the generation and the accumulation of biogenic amine.
Summary of the invention
The objective of the invention is to: can produce biogenic amine to producing the amine bacterium in the meat product fermentative prodn; Screen and a kind ofly can in the meat product fermentative prodn, produce the biogenic amine oxydase; Suppress to produce the growth of amine bacterium, the lactobacterium plantarum strain and the application thereof of oxidation and degradation biological amine.
The objective of the invention is to realize like this: a lactobacillus plantarum (Lactobacillus plantarum) xlbf002, it is characterized in that: the preserving number of bacterial strain is CGMCC No.3167, bacterial strain is cultivated 36h for 37 ℃ on the MRS nutrient agar; Enrichment in MRS liquid culture medium, white colony, straight-bar becomes short chain; Gram-positive, atrichia, homofermentation; Glucose fermentation is aerogenesis not, and fermentation produces acetate and lactic acid during ribose, does not reduce nitrite; This bacterial strain is shaft-like under simple microscope, the Genbank number of landing of this bacterial strain 16S rDNA is GQ890358.
In the present invention: described MRS nutrient agar is: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, hydrogen citrate three ammonium 2g; Glucose 20g, tween 80 1mL, sodium acetate 5g; Potassium hydrogenphosphate 2g, sal epsom 0.5g, manganous sulfate 0.25g; Agar 18 grams, zero(ppm) water 1000mL, pH value 6.2～6.4; Described MRS liquid culture medium is: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, hydrogen citrate three ammonium 2g; Glucose 20g, tween 80 1mL, sodium acetate 5g, potassium hydrogenphosphate 2g; Sal epsom 0.5g, manganous sulfate 0.25g, lime carbonate 0.4g, cysteine hydrochloride; Milk extract powder 7.5g, beer 65ml, zero(ppm) water 1000mL, pH value 6.2～6.4.
The application of an above-mentioned lactobacillus plantarum is characterized in that: with the bacterial strain activation, spread cultivation, form starter behind the microorganism collection, in the meat product fermentative prodn, produce the biogenic amine oxydase, suppress to produce growth, oxidation and the degradation biological amine of amine bacterium.
In the application of a said lactobacillus plantarum: described bacterial strain activation is meant: be inoculated in the test tube that liquid nutrient medium is housed from the inclined-plane picking colony; 37 ℃ left standstill cultivation after 24 hours; Get bacterium liquid and be inoculated in the test tube that MRS liquid culture medium is housed 37 ℃ and leave standstill and cultivated 22 hours, the inoculum size in the test tube is 10
6Cfu/ml gets bacterium liquid inoculation activation three times at least repeatedly; Described spreading cultivation is meant: activatory bacterium liquid is inoculated in the triangular flask that MRS liquid culture medium is housed, and the inoculum size in the triangular flask is 10
6Cfu/ml, 37 ℃ leave standstill cultivation 22 hours, form the bacterium liquid of enrichment bacterial classification; Described microorganism collection is meant: the bacterium liquid of getting the enrichment bacterial classification is in aseptic centrifuge tube, and 10000 left the heart 10 minutes, and abandoning supernatant obtains thalline under aseptic condition, and the thalline of acquisition can directly be used as starter, also can process dry powder leaven.
In the application of a said lactobacillus plantarum: described starter is meant: the thalline of collecting is washed out directly as starter with sterile physiological, or in thalline, added the protective material postlyophilization, process dry powder leaven.
In the application of a said lactobacillus plantarum: described product amine bacterium is meant: faecium, enterococcus faecalis, enteroaerogen, Escherichia coli, enterobacter cloacae; The biogenic amine that said product amine bacterium produces is meant: tyrasamine, putrescine, cadaverine, phenylethylamine; The biogenic amine of the biogenic amine oxydase institute oxidative degradation that produces is putrescine and cadaverine.
In the application of a said lactobacillus plantarum: the add-on of starter is 10 in the prescription of meat product fermentative prodn
6-7Cfu/g need add 1.5% glucose simultaneously.
The invention has the advantages that: a lactobacillus plantarum (Lactobacillus plantarum) xlbf002 energy for growth is strong, after the enrichment culture in the substratum cell concentration reach 10
12Cfu/ml; Starter preparation technology is simple, and the thalline that obtains is placed protective material, can when the production dry powder leaven, reduce the inactivation rate of plant lactobacillus; Starter can effectively suppress the growth and breeding of enterobacteria in the meat product fermentative prodn; The generation of control biogenic amine, the biogenic amine oxydase of this bacterial strain generation can reduce the accumulation of biogenic amine in addition.
Description of drawings
Fig. 1 is the picture of plant lactobacillus (Lactobacillus plantarum) xlbf002 bacterial strain under simple microscope.
Embodiment
Embodiment 1
The screening of bacterial strain and preservation
This bacterial strain is from the traditional Chinese sausage, to separate the bacterial strain that does not produce biogenic amine, and it is centrifugal to be separated to strain cultured solution, gets supernatant, observes supernatant to five kinds of inhibition abilities of producing the amine bacterium.And its access contained in the substratum of biogenic amine, detect oxidation and reduce the biogenic amine ability.
This Pseudomonas gram-positive microorganism, white colony, straight-bar becomes short chain, atrichia, homofermentation, glucose fermentation is aerogenesis not, produces acetate and lactic acid during fermentation ribose, does not reduce nitrite, edwardsiella hoshinae, hydrogen sulfide produces negative, coagulase-negative; This bacterial strain is shaft-like under simple microscope.
This bacterial strain is according to Gen bank comparison result; Called after plant lactobacillus (Lactobacillus plantarum) xlbf002; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on July 6th, 2009, preserving number is CGMCC No.3167.Universal primer U968, the Genbank number of landing of this bacterial strain 16S rDNA of L1401 amplification is GQ890358.
Embodiment 2
Plant lactobacillus (Lactobacillus plantarum) xlbf002 uses the restraining effect experiment of producing the amine bacterium and produces the amine bacterium: faecium
Substratum: MRS nutrient agar (peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, hydrogen citrate three ammonium 2g, glucose 20g; Tween 80 1mL, sodium acetate 5g, potassium hydrogenphosphate 2g, sal epsom 0.5g; Manganous sulfate 0.25g, agar 18 grams, zero(ppm) water 1000mL, pH value 6.2-6.4.
Experimental technique
Advantage in the traditional Chinese sausage is produced the petridish that amine intestines bacteruria faecalis is coated MRS agar; With plant lactobacillus (Lactobacillus plantarum) the xlbf002 bacterium liquid of 37 ℃ of cultivations in 24 hours, 8000 left the heart 10 minutes, drew supernatant in aseptic centrifuge tube with aseptic rifle head.Get sterilized diameter and pick this supernatant, and be positioned on the MRS nutrient agar of above-mentioned inoculation enterobacteria, observe inhibition zone for 0.6cm filter paper.Inhibition zone is greater than 1.0cm, explains that xlbf002 has restraining effect to faecium.
Use the same method respectively to enterococcus faecalis, enteroaerogen, Escherichia coli and enterobacter cloacae, inhibition zone proves that all greater than 1.0cm plant lactobacillus xlbf002 has identical restraining effect to above-mentioned product amine bacterium.
Embodiment 3
The effect of plant lactobacillus (Lactobacillus plantarum) xlbf002 oxidation and degradation biological amine.
Biogenic amine: putrescine and cadaverine
Substratum: the training of modified MRS liquid (peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, hydrogen citrate three ammonium 2g; Glucose 20g, tween 80 1mL, sodium acetate 5g, potassium hydrogenphosphate 2g; Sal epsom 0.5g, manganous sulfate 0.25g, lime carbonate 0.4g, cysteine hydrochloride; Milk extract powder 7.5g, beer 65ml, zero(ppm) water 1000mL, pH value 6.2～6.4.
Experimental technique
Experiment is divided into three groups, corresponds respectively to putrescine, cadaverine and control group, the biogenic amine that in the MRS liquid culture medium of each group, adds 500ug/ml respectively with blank as contrast, 115 ℃ of sterilization 15min; Cooling back experimental group inserts plant lactobacillus (Lactobacillus plantarum) xlbf002, and inoculum size is 10
6Cfu/ml, 37 ℃, shaking table (85 rev/mins) was cultivated 72 hours, and HPLC surveys the variation of respectively organizing biogenic amine concentration, and wherein putrescine has reduced by 144.87 μ g/ml, and cadaverine has reduced by 146.42 μ g/ml.Explain that plant lactobacillus xlbf002 is obvious to the oxidation and the Degradation of biogenic amine.
Embodiment 4
The collection of thalline
Substratum: the training of modified MRS liquid (peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract paste 5g, hydrogen citrate three ammonium 2g; Glucose 20g, tween 80 1mL, sodium acetate 5g, potassium hydrogenphosphate 2g; Sal epsom 0.5g, manganous sulfate 0.25g, lime carbonate 0.4g, cysteine hydrochloride; Milk extract powder 7.5g, beer 65ml, zero(ppm) water 1000mL, pH value 6.2～6.4.
Take by weighing above-mentioned substratum, put in the 800ml zero(ppm) water, heat and stir to fully the dissolving after, moisturizing is to 1000ml.After stirring once more, be sub-packed in the test tube, and, sterilize, use in order to activation with behind the rubber sieve jam-pack.Ready-made substratum is sub-packed in the triangular flask, is used to after the sterilization spread cultivation.
1, activation
Be inoculated in the test tube that liquid nutrient medium is housed from the inclined-plane picking colony, 37 ℃ leave standstill and cultivate after 24 hours, get bacterium liquid and are inoculated in the test tube that MRS liquid culture medium is housed 37 ℃ and leave standstill and cultivated 22 hours, and the inoculum size in the test tube is 10
6Cfu/ml gets bacterium liquid inoculation activation three times at least repeatedly.
2, spread cultivation
Activatory bacterium liquid is inoculated in the triangular flask that MRS liquid culture medium is housed, and the inoculum size in the triangular flask is 10
6Cfu/ml, 37 ℃ leave standstill cultivation 22 hours, form the bacterium liquid of enrichment bacterial classification.
3, microorganism collection
The bacterium liquid of getting the enrichment bacterial classification is in aseptic centrifuge tube, and 10000 left the heart 10 minutes, abandoning supernatant under the aseptic condition.Promptly collect thalline.
Embodiment 5
The preparation of starter
1, the thalline of collecting is washed out with sterile physiological, formed starter.
2, the thalline of collecting is placed protective material, process dry powder leaven, wherein, protective material is skimming milk+glycerine 50mL/L+ sucrose 30g/L5+ maltodextrin 10g/L+ Sodium Glutamate 20g/L+ yeast lixiviate cream 20g/L of 15%.
Embodiment 6
Application in the meat product fermentative prodn (is example with the guang-type sausage)
Prescription: peeling pork, lean to fat ratio is 1: 3, contains nitre refined salt 2.5% (adding the 6g Sodium Nitrite in the per kilogram salt) glucose 1.5%, sucrose 2%, light soy sauce 1%, Fenyang wine 4%, spice is an amount of, the starter 10 of preparation among the embodiment 5
6Cfu/g.
Working method:
1. fat meat is cut into about 0.5cm
3, put into 100 ℃ of boiling water and soaked 1 hour, remove oil slick with cold water flush, drain, mix Fenyang wine, sucrose was pickled 8 hours for 4 ℃～6 ℃.
2. rub or be cut into by knife the fragment of soybean grain size to lean meat with mincer, add and contain nitre refined salt, spice, light soy sauce and glucose are mixed all, pickle 24 hours for 4 ℃～6 ℃.The back adds starter and mixes all.
3. fat and lean meat is put together and mix thoroughly, after the bowel lavage,, and prick the hole on intestines surfaces, be convenient to venting with pin with warm water flush away intestines surface oil slick.
4. behind the draining, Exposure to Sunlight or sootiness were dried in the shade ripe one month, i.e. edible after 2～3 days.
In the guang-type sausage sample with the present invention's production, the biogenic amine total amount is lower than more than 60% of producing the same period of guang-type sausage sample (calling control group in the following text) with starter.The sample maturation that the present invention produces detects less than enterobacteria when finishing, and control sample midgut amount of bacteria is 10
2Cfu/g.Milk-acid bacteria content was 10 when maturation finished in the sample that the present invention produces
8Cfu/g, lactic acid bacteria number is 10 in the control sample
5Cfu/g.It is thus clear that the rapid breeding of plant lactobacillus xlbf002 can effectively suppress enterobacteria, suppress biogenic amine and produce, reduce the biogenic amine accumulation.
More than each embodiment be not to concrete restriction of the present invention.
Claims (5)
1. a lactobacillus plantarum (Lactobacillus plantarum), it is characterized in that: the preserving number of bacterial strain is CGMCC No.3167, bacterial strain is cultivated 36h for 37 ℃ on the MRS nutrient agar; Enrichment in MRS liquid culture medium, white colony, straight-bar becomes short chain; Gram-positive, atrichia, homofermentation; Glucose fermentation is aerogenesis not, and fermentation produces acetate and lactic acid during ribose, does not reduce nitrite; This bacterial strain is shaft-like under simple microscope, the Genbank number of landing of this bacterial strain 16S rDNA is GQ890358.
2. application that preserving number is the lactobacillus plantarum of CGMCC No.3167; It is characterized in that: with the bacterial strain activation, spread cultivation, form starter behind the microorganism collection; In the meat product fermentative prodn, suppress to produce the growth of amine bacterium, produce the biogenic amine oxydase, the oxidative degradation biogenic amine;
Described product amine bacterium is meant: faecium, enterococcus faecalis, enteroaerogen, Escherichia coli, enterobacter cloacae; The biogenic amine of the biogenic amine oxydase oxidation-degradable that this bacterial strain produced is: putrescine and cadaverine.
3. preserving number according to claim 2 is the application of the lactobacillus plantarum of CGMCC No.3167; It is characterized in that: described bacterial strain activation is meant: be inoculated in the test tube that liquid nutrient medium is housed from the inclined-plane picking colony; 37 ℃ left standstill cultivation after 24 hours; Get bacterium liquid and be inoculated in the test tube that MRS liquid culture medium is housed 37 ℃ and leave standstill and cultivated 22 hours, the inoculum size in the test tube is 10
6Cfu/ml gets bacterium liquid inoculation activation three times at least repeatedly; Described spreading cultivation is meant: activatory bacterium liquid is inoculated in the triangular flask that MRS liquid culture medium is housed, and the inoculum size in the triangular flask is 10
6Cfu/ml, 37 ℃ leave standstill cultivation 22 hours, form the bacterium liquid of enrichment bacterial classification; Described microorganism collection is meant: the bacterium liquid of getting the enrichment bacterial classification is in aseptic centrifuge tube, and 10000 left the heart 10 minutes, and abandoning supernatant obtains thalline under aseptic condition; The thalline that obtains is directly used as starter, or processes dry powder leaven.
4. preserving number according to claim 3 is the application of the lactobacillus plantarum of CGMCC No.3167; It is characterized in that: the thalline of described acquisition is directly used as starter; Or process dry powder leaven and be meant: the thalline of collecting is washed out directly as starter with sterile physiological; Or in thalline, add the protective material postlyophilization, process dry powder leaven.
5. be the application of the lactobacillus plantarum of CGMCC No.3167 according to the described preserving number of one of claim 2～4, it is characterized in that: the add-on of starter is 10 in the prescription of meat product fermentative prodn
6-7Cfu/g need add 1.5% glucose simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910232713XA CN101701203B (en) | 2009-11-27 | 2009-11-27 | Lactobacillus plantarum and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910232713XA CN101701203B (en) | 2009-11-27 | 2009-11-27 | Lactobacillus plantarum and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101701203A CN101701203A (en) | 2010-05-05 |
| CN101701203B true CN101701203B (en) | 2012-06-06 |
Family
ID=42156130
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910232713XA Active CN101701203B (en) | 2009-11-27 | 2009-11-27 | Lactobacillus plantarum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101701203B (en) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732447B (en) * | 2012-04-18 | 2013-11-13 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum for inhibiting mould and yeasts in bread, and application method thereof |
| CN102703345B (en) * | 2012-05-10 | 2013-10-16 | 江南大学 | Lactobacillus coryniformis for inhibiting production of biogenic amine and applications of lactobacillus coryniformis |
| CN103005474A (en) * | 2012-08-20 | 2013-04-03 | 青岛农业大学 | Method for preparing meat fermented bean curd by moulds |
| CN103704741A (en) * | 2014-01-03 | 2014-04-09 | 扬州大学 | Culture method of leavening agent for high-density goose meat sausages |
| CN106244383B (en) * | 2016-10-13 | 2019-04-02 | 福建农林大学 | The integrated processing method of Content of Biogenic Amines in a kind of reduction yellow rice wine |
| CN107663509B (en) * | 2017-09-12 | 2020-12-08 | 北京工商大学 | Application of lactobacillus casei in preparation of sausage, method for preparing sausage and sausage |
| CN108300669B (en) * | 2018-03-26 | 2020-09-01 | 贵州大学 | Fermented bean curd ripening fermentation complex microbial inoculant and preparation method thereof |
| CN109321486B (en) * | 2018-09-18 | 2020-07-10 | 广东工业大学 | Lactic acid bacteria capable of degrading nitrite and biogenic amine and application thereof |
| CN109306332B (en) * | 2018-09-22 | 2021-06-01 | 南京农业大学 | Lactobacillus fermentum CD110 and application thereof in preparation of fermented sausages |
| CN109588656A (en) * | 2018-12-07 | 2019-04-09 | 东北农业大学 | A method of utilizing degradable biological amine lactobacillus plantarum fermented vegetable |
| CN110042069B (en) * | 2019-01-09 | 2022-10-04 | 贵州师范大学 | Pig-derived lactobacillus plantarum for reducing cholesterol and application thereof |
| CN109735461B (en) * | 2019-01-11 | 2022-11-11 | 大连工业大学 | Lactobacillus plantarum and application thereof in reducing biogenic amine content of fish tea |
| CN109971670B (en) * | 2019-02-01 | 2023-01-24 | 温州医科大学 | Enterococcus faecalis, culture medium thereof, and method for detecting antibiotic residues in milk and application thereof |
| CN110499271B (en) * | 2019-09-02 | 2021-11-23 | 千禾味业食品股份有限公司 | Lactobacillus plantarum QR19 and application thereof |
| CN110982863B (en) * | 2019-12-31 | 2023-02-03 | 广州舒国生物科技有限公司 | Microbial fermentation method for producing flavor nucleotide disodium |
| CN111454858A (en) * | 2020-04-03 | 2020-07-28 | 大连工业大学 | Method for inhibiting biogenic amine accumulation of amine-producing bacteria by using hurdle technology |
| CN112195119B (en) * | 2020-09-23 | 2022-08-05 | 西南大学 | Lactobacillus plantarum capable of degrading biogenic amine and resisting salt and application thereof |
| CN113142292A (en) * | 2020-12-29 | 2021-07-23 | 湖州展旺食品科技有限公司 | Method for prolonging preservation period of cold fresh meat by using antibacterial lactobacillus composite preservation solution |
| CN113068782B (en) * | 2021-04-01 | 2023-03-28 | 新疆农业科学院微生物应用研究所(中国新疆—亚美尼亚生物工程研究开发中心) | Brown active lactobacillus beverage and preparation method thereof |
| CN113817661A (en) * | 2021-09-04 | 2021-12-21 | 芜湖职业技术学院 | Culture method for improving antibacterial activity of lactobacillus plantarum |
| CN113684155A (en) * | 2021-09-13 | 2021-11-23 | 吉林大学 | Bacterial strain with biogenic amine reducing effect and application |
| CN114395512B (en) * | 2022-02-22 | 2024-01-16 | 宁夏大学 | Lactic acid bacteria capable of degrading biogenic amine and application thereof |
-
2009
- 2009-11-27 CN CN200910232713XA patent/CN101701203B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101701203A (en) | 2010-05-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101701203B (en) | Lactobacillus plantarum and application thereof | |
| CN101724591B (en) | Lactobacillus plantarum strain and application thereof | |
| CN105132308B (en) | A kind of Lactobacillus plantarum that can reduce biogenic amine in food content and its application | |
| CN102899262B (en) | Lactobacillus plantarum and method for fast degrading nitrite produced in fermentation by the lactobacillus plantarum | |
| CN105441349B (en) | One plant of novel plant lactobacillus and its application | |
| CN104531578A (en) | Lactobacillus plantarum strain having functions of effectively degrading nitrite and strongly producing acid and application of lactobacillus plantarum strain | |
| CN102018154A (en) | Method for inoculating lactobacillus to rapidly reduce nitrite content in pickles | |
| CN109735461B (en) | Lactobacillus plantarum and application thereof in reducing biogenic amine content of fish tea | |
| CN105105119B (en) | It is a kind of that the method that fermentation prepares fruit ferment is carried out based on inoculation strain | |
| CN106754472A (en) | One plant of fruits and vegetables fermentation Lactobacillus plantarum grx15 and its application | |
| CN105779332A (en) | Liquid composite lactic acid bacteria fermentation agent and preparation method and application thereof | |
| CN105249365A (en) | Method for inhibiting pickled vegetables from mildewing | |
| RU2005104426A (en) | MEGACPHAERA ELSDENII STRAIN AND ITS APPLICATION | |
| CN104605337B (en) | A kind of processing method of flavor fermentation pleurotus eryngii | |
| CN109593684A (en) | One lactobacillus plantarum Yc-5 and its application in reduction fish tea Content of Biogenic Amines | |
| CN108004177B (en) | Lactobacillus paracasei capable of degrading nitrite and characteristic research thereof | |
| CN110584032A (en) | Method for biologically removing fishy smell of shellfish meat through ozone combined with anaerobic microbial fermentation | |
| CN106244498B (en) | Preparation method of fruit and vegetable pesticide residue purification microbial inoculum | |
| CN110760456B (en) | Lactobacillus plantarum La1 for degrading cholesterol and application thereof | |
| CN104371957A (en) | Bacillus pumilus viable preparation and solid fermentation method and applications thereof | |
| Wafula | Effects of postharvest-processing technologies on the safety and quality of African indigenous leafy vegetables | |
| CN108265019B (en) | Leuconostoc mesenteroides subsp mesenteroides, preparation method and application | |
| CN114231464B (en) | Bacillus coagulans and application thereof | |
| CN109717413A (en) | A kind of preparation method of wet salting Spanish mackerel | |
| CN112715890B (en) | Immobilized pickle starter and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |