CN109721385A - A kind of microbial-bacterial fertilizer for fruit expanding period - Google Patents
A kind of microbial-bacterial fertilizer for fruit expanding period Download PDFInfo
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- CN109721385A CN109721385A CN201910223309.XA CN201910223309A CN109721385A CN 109721385 A CN109721385 A CN 109721385A CN 201910223309 A CN201910223309 A CN 201910223309A CN 109721385 A CN109721385 A CN 109721385A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The present invention provides a kind of microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: 70-80 parts of kitchen garbage, 5-15 parts of silicate bacteria fermentation liquid, 10-20 parts of saccharomycete bacterium powder, 4-8 parts of Trichoderma bacterium powder, 10-20 parts of aspergillus oryzae fermentation liquid.The microbial-bacterial fertilizer can decompose the organic nitrogen in kitchen garbage and soil, make in bacterial manure rich in the nitrogen, phosphorus, potassium element for being easy to plant absorption；A large amount of antibacterial material can also be provided, the harmful virus in soil is avoided to generate harm to fruit tree；It by using microbial-bacterial fertilizer provided by the invention, can turn waste into wealth, provide enough nitrogen, calcium, potassium and microelement for fruit expanding period, improve fruit in the surface degree of staining, the diameter of fruit and the weight of fruit of expanding stage.
Description
Technical field
The present invention relates to bio-bacterial manure technical field, specially a kind of microbial-bacterial fertilizer for fruit expanding period.
Background technique
In recent years, as the pursuit to fruit yield is higher and higher, a large amount of usage amounts of chemical fertilizer are increasing, cause soil
Hardened, soil pollution phenomena such as, sustainable development and the use of soil are affected, although rich in a large amount of organic in soil
Object, but can not be absorbed by fruit tree, cause waste material waste.Tree Fruit increases the demand of nutrition in expanding stage,
The demand that the cell quantity for meeting fruit increases and cell volume increases, in the prior art, to meet expanding stage demand, to
It is produced and continues to apply chemical fertilizer in the soil of soil pollution and although meeting current demand form the evil of soil
Property circulation, due to excessive chemical fertilizer can to the root of fruit tree generate calcination phenomena such as, cause the growth of fruit expanding period can not
Develop towards expected direction, wastes a large amount of human and material resources and financial resources.
As the improvement of people's living standards, kitchen garbage largely generates, living environment is drastically influenced, in kitchen garbage
It can turn waste into wealth after rationally utilizing it rich in vitamin, protein, microelement etc. substances, provide battalion for the growth of fruit tree
Substance is supported, grows and uses for fruit expanding period.
Summary of the invention
In consideration of it, the microbial-bacterial fertilizer can will eat the present invention provides a kind of microbial-bacterial fertilizer for fruit expanding period
Organic nitrogen in kitchen rubbish and soil is decomposed, and is made in bacterial manure rich in the nitrogen, phosphorus, potassium element for being easy to plant absorption；It can be with
A large amount of antibacterial material is provided, the harmful virus in soil is avoided to generate harm to fruit tree；By using provided by the invention micro-
Bio-bacterial manure can turn waste into wealth, and provide enough nitrogen, calcium, potassium and microelement for fruit expanding period, improve fruit and expanding
Surface degree of staining, the diameter of fruit and the weight of fruit of phase.
Technical scheme is as follows:
A kind of microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: kitchen garbage 70-80
Part, 5-15 parts of silicate bacteria fermentation liquid, 10-20 parts of saccharomycete bacterium powder, 4-8 parts of Trichoderma bacterium powder, aspergillus oryzae fermentation liquid 10-20
Part.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: 75 parts of kitchen garbage, silicon
13 parts of hydrochlorate ferment product, 15 parts of saccharomycete bacterium powder, 6 parts of Trichoderma bacterium powder, 15 parts of aspergillus oryzae fermentation liquid.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the bacteria concentration in the silicate bacteria fermentation liquid is 1,000,000,000/
ml。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the silicate bacteria fermentation liquid are LB liquid
Culture medium prepares seed, is inoculated in fermentation medium, under the conditions of 30 DEG C, cultivates 36-46h.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the fermentation medium are 30g maize cob meal, 20g kitchen rubbish
Rubbish, CaCO30.10g, Na2HPO40.20g, FeCl30.05g, MgSO40.50g, pH6-7 are settled to 1000mL, sterilizing.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the viable count of the saccharomycete bacterium powder are 10,000,000,000/g；The wood
The viable count of mould bacterium powder is 10,000,000,000/g.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the spore concentration in the aspergillus oryzae fermentation liquid is 10,000,000,000/
ml。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the aspergillus oryzae fermentation liquid are to be trained using seed
Feeding base prepares seed, then accesses fermentation medium and ferments, ferment 3d under the conditions of 30 DEG C.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the seed culture medium are as follows: maltose 15g, tryptone
10g、(NH4)2HPO420g, NaCl 10g, yeast extract 5g, pH7.0-7.5 are settled to 1L, shaking flask culture；The fermentation
Culture medium is starch 50g, yeast extract 3g, beancake powder 5g, KH2PO42g, MgSO4·7H2O 0.5g。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period the preparation method comprises the following steps:
(1) kitchen garbage is subjected to washing desalination, degreasing, crushed, cross 200 meshes, and high-temperature sterilization under the conditions of 120 DEG C
30-50min is let cool to room temperature, adjusts pH6-7, spare；
(2) saccharomycete bacterium powder, Trichoderma bacterium powder are put into respectively in step (1) smashed kitchen garbage, in 30 DEG C of temperature
It ferments under the conditions of degree, fermentation time 3-4d obtains karusen I, spare；
(3) silicate bacteria fermentation liquid and aspergillus oryzae fermentation liquid are put into karusen I, are carried out under the conditions of 30 DEG C of temperature
Fermentation adjusts pH6.6-7.4 until stopping fermentation when the viable count in karusen is 5,000,000,000/g, obtains microbial-bacterial fertilizer.
Kitchen garbage is the sanitary waste that people generate in personal consumption, mainly includes the food residues such as rice and flour,
Chemically divide on composition, there is starch, protein, cellulose and inorganic salts etc., these matter-poles are perishable and generate to environment dirty
Dye, and due to being the mixture of solid and liquid, existing Food waste treatment method is mostly by solid refuse and liquid
Rubbish is separated, and is respectively processed after separation, troublesome in poeration.The present invention by kitchen garbage by directly crushing, sterilizing
Mode utilizes solid portion and liquid portion simultaneously, reduces the processing step of kitchen garbage, while passing through sterilization process,
The bacterium in rubbish and viral transmission are avoided, the safety that kitchen garbage uses is improved.In addition, passing through meal after sterilization
Microorganism is added in the rubbish of kitchen, on the one hand the organic matter etc. in kitchen garbage is decomposed, on the other hand prevents kitchen garbage
Harmful virus and the bacterium for endangering fruit tree growth are inside generated again, improve safety, the feature of environmental protection that microbial-bacterial fertilizer uses.
The prior art is compared, the beneficial effects of the present invention are:
1, in the present invention, by using kitchen garbage, reduce environmental pressure, kitchen garbage is turned waste into wealth, save
The energy, while enough nutriments are provided for fruit expanding period growth.
2, by the present invention in that with silicate bacteria, saccharomycete, Trichoderma and aspergillus oryzae, not only can to kitchen garbage and
Organic matter in soil is decomposed, and provides nutrition for fruit tree growth, meanwhile, can recuperate to avoid microbial-bacterial fertilizer intussusception poison and
Harmful bacteria guarantees safety and the feature of environmental protection when fruit tree uses.
3, it is experimentally confirmed, using microbial-bacterial fertilizer provided by the invention, increases effectively the yield of fruit, improves
The coloring ratio of fruit surface, the diameter for improving single fruit.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, below in conjunction with of the invention real
Example is applied, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is only
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without making creative work, should fall within the scope of the present invention.
In 1-3 of the embodiment of the present invention, silicate bacteria is colloid bacillus cereus；Bacterium in colloid bacillus cereus fermentation liquid is dense
Degree is 1,000,000,000/ml；The viable count of saccharomycete bacterium powder is 10,000,000,000/g；The viable count of the Trichoderma bacterium powder is 10,000,000,000/g；Meter Qu
Spore concentration in mould fermentation liquid is 10,000,000,000/ml.
Embodiment 1
A kind of microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: 75 parts of kitchen garbage, glue
13 parts of matter fermentation of bacillus liquid, 15 parts of saccharomycete bacterium powder, 6 parts of Trichoderma bacterium powder, 15 parts of aspergillus oryzae fermentation liquid.
In the above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of colloid bacillus cereus fermentation liquid is LB liquid
Culture medium prepares seed, is inoculated in fermentation medium, under the conditions of 30 DEG C, cultivates 41h.
In the above-mentioned microbial-bacterial fertilizer for fruit expanding period, fermentation medium be 30g maize cob meal, 20g kitchen garbage,
CaCO30.10g, Na2HPO40.20g, FeCl30.05g, MgSO40.50g, pH6.5 are settled to 1000mL, sterilizing.
In the above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of aspergillus oryzae fermentation liquid is using seed culture
Base prepares seed, then accesses fermentation medium and ferments, ferment 3d under the conditions of 30 DEG C, wherein seed culture medium are as follows: wheat
Bud sugar 15g, tryptone 10g, (NH4)2HPO420g, NaCl 10g, yeast extract 5g, pH7.0-7.5, are settled to 1L, shake
Bottle culture；Fermentation medium is starch 50g, yeast extract 3g, beancake powder 5g, KH2PO42g, MgSO4·7H2O 0.5g。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period the preparation method comprises the following steps:
(1) kitchen garbage is subjected to washing desalination, degreasing, crushed, cross 200 meshes, and high-temperature sterilization under the conditions of 120 DEG C
40min is let cool to room temperature, adjusts pH6.5, spare；
(2) saccharomycete bacterium powder, Trichoderma bacterium powder are put into respectively in step (1) smashed kitchen garbage, in 30 DEG C of temperature
It ferments under the conditions of degree, fermentation time 3.5d obtains karusen I, spare；
(3) colloid bacillus cereus fermentation liquid and aspergillus oryzae fermentation liquid are put into karusen I, under the conditions of 30 DEG C of temperature into
Row fermentation adjusts pH7.0, the microbial-bacterial fertilizer of acquisition until stopping fermentation when the viable count in karusen is 5,000,000,000/g.
Embodiment 2
A kind of microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: 70 parts of kitchen garbage, glue
5 parts of matter fermentation of bacillus liquid, 10 parts of saccharomycete bacterium powder, 4 parts of Trichoderma bacterium powder, 10 parts of aspergillus oryzae fermentation liquid.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the colloid bacillus cereus fermentation liquid are LB liquid
Body culture medium prepares seed, is inoculated in fermentation medium, under the conditions of 30 DEG C, cultivates 36h.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the fermentation medium are 30g maize cob meal, 20g kitchen rubbish
Rubbish, CaCO30.10g, Na2HPO40.20g, FeCl30.05g, MgSO40.50g, pH6 are settled to 1000mL, sterilizing.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the aspergillus oryzae fermentation liquid are to be trained using seed
Feeding base prepares seed, then accesses fermentation medium and ferments, ferment 3d under the conditions of 30 DEG C；Wherein, seed culture medium are as follows:
Maltose 15g, tryptone 10g, (NH4)2HPO420g, NaCl 10g, yeast extract 5g, pH7.0, are settled to 1L, shaking flask
Culture；Fermentation medium is starch 50g, yeast extract 3g, beancake powder 5g, KH2PO42g, MgSO4·7H2O 0.5g。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period the preparation method comprises the following steps:
(1) kitchen garbage is subjected to washing desalination, degreasing, crushed, cross 200 meshes, and high-temperature sterilization under the conditions of 120 DEG C
30min is let cool to room temperature, and adjusting pH is 6, spare；
(2) saccharomycete bacterium powder, Trichoderma bacterium powder are put into respectively in step (1) smashed kitchen garbage, in 30 DEG C of temperature
It ferments under the conditions of degree, fermentation time 3d obtains karusen I, spare；
(3) colloid bacillus cereus fermentation liquid and aspergillus oryzae fermentation liquid are put into karusen I, under the conditions of 30 DEG C of temperature into
Row fermentation adjusts pH6.6, the microbial-bacterial fertilizer of acquisition until stopping fermentation when the viable count in karusen is 5,000,000,000/g.
Embodiment 3
A kind of microbial-bacterial fertilizer for fruit expanding period, the component including following portions by weight: 80 parts of kitchen garbage, glue
15 parts of matter fermentation of bacillus liquid, 20 parts of saccharomycete bacterium powder, 8 parts of Trichoderma bacterium powder, 20 parts of aspergillus oryzae fermentation liquid.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the colloid bacillus cereus fermentation liquid are LB liquid
Body culture medium prepares seed, is inoculated in fermentation medium, under the conditions of 30 DEG C, cultivates 46h；Wherein, fermentation medium is 30g beautiful
Rice core powder, 20g kitchen garbage, CaCO30.10g, Na2HPO40.20g, FeCl30.05g, MgSO40.50g, pH7 are settled to
1000mL, sterilizing.
The above-mentioned microbial-bacterial fertilizer for fruit expanding period, the preparation method of the aspergillus oryzae fermentation liquid are to be trained using seed
Feeding base prepares seed, then accesses fermentation medium and ferments, ferment 3d under the conditions of 30 DEG C, wherein seed culture medium are as follows:
Maltose 15g, tryptone 10g, (NH4)2HPO420g, NaCl 10g, yeast extract 5g, pH7.0-7.5, are settled to 1L,
Shaking flask culture；The fermentation medium is starch 50g, yeast extract 3g, beancake powder 5g, KH2PO42g, MgSO4·7H2O
0.5g。
The above-mentioned microbial-bacterial fertilizer for fruit expanding period the preparation method comprises the following steps:
(1) kitchen garbage is subjected to washing desalination, degreasing, crushed, cross 200 meshes, and high-temperature sterilization under the conditions of 120 DEG C
50min is let cool to room temperature, and adjusting pH is 7, spare；
(2) saccharomycete bacterium powder, Trichoderma bacterium powder are put into respectively in step (1) smashed kitchen garbage, in 30 DEG C of temperature
It ferments under the conditions of degree, fermentation time 4d obtains karusen I, spare；
(3) colloid bacillus cereus fermentation liquid and aspergillus oryzae fermentation liquid are put into karusen I, under the conditions of 30 DEG C of temperature into
Row fermentation adjusts pH7.4, the microbial-bacterial fertilizer of acquisition until stopping fermentation when the viable count in karusen is 5,000,000,000/g.
The microbial-bacterial fertilizer that embodiment 1-3 is obtained is applied to apple tree, and the use when apple enters expanding stage, chooses
The age of tree is identical, totally 40, the comparable fruit tree of fruit amount, is divided into four groups, and respectively group A, group B, group C and group D, group A is as control
Group does not use chemical fertilizer；Group B, group C and group D are experimental group, apply the embodiment of the present invention 1, embodiment 2 and embodiment 3 respectively and distinguish
The microbial-bacterial fertilizer of offer is on the same day picking fruit, and is recording the features such as the weight of fruit, appearance, see the table below:
1 fruit of table record
Note: [1] refers to the percentage of fruit number Zhan total fruit number of the fruit diameter more than or equal to 85cm；
[2] rate of growth (%)=(total output-control group total output of experimental group)/control group total output × 100.
As can be seen from the above table, the microbial-bacterial fertilizer present invention improved is used in fruit expanding period, can effectively improve big fruit
Quantity, and the appearance of fruit is obviously improved, and red overlay area reaches 90% or more, improve the quality and yield of fruit,
Rate of growth reaches 63% or more.
Claims (10)
1. a kind of microbial-bacterial fertilizer for fruit expanding period, which is characterized in that the component including following portions by weight: kitchen rubbish
70-80 parts of rubbish, 5-15 parts of silicate bacteria fermentation liquid, 10-20 parts of saccharomycete bacterium powder, 4-8 parts of Trichoderma bacterium powder, aspergillus oryzae fermentation
10-20 parts of liquid.
2. being used for the microbial-bacterial fertilizer of fruit expanding period as described in claim 1, which is characterized in that including following portions by weight
Component: 75 parts of kitchen garbage, 13 parts of silicate bacteria fermentation liquid, 15 parts of saccharomycete bacterium powder, 6 parts of Trichoderma bacterium powder, aspergillus oryzae
15 parts of fermentation liquid.
3. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 1 or 2, which is characterized in that the silicate is thin
Bacteria concentration in fermented liquid is 1,000,000,000/ml.
4. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 3, which is characterized in that the silicate bacteria hair
The preparation method of zymotic fluid is that LB liquid medium prepares seed, is inoculated in fermentation medium, under the conditions of 30 DEG C, cultivates 36-
46h。
5. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 4, which is characterized in that the fermentation medium is
30g maize cob meal, 20g kitchen garbage, CaCO30.10g, Na2HPO40.20g, FeCl30.05g, MgSO40.50g, pH6-
7, it is settled to 1000mL, is sterilized.
6. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 1 or 2, which is characterized in that the saccharomycete bacterium
The viable count of powder is 10,000,000,000/g；The viable count of the Trichoderma bacterium powder is 10,000,000,000/g.
7. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 1 or 2, which is characterized in that the aspergillus oryzae hair
Spore concentration in zymotic fluid is 10,000,000,000/ml.
8. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 7, which is characterized in that the aspergillus oryzae fermentation liquid
Preparation method be that seed prepared using seed culture medium, then access fermentation medium and ferment, issued in 30 DEG C of conditions
Ferment 3d.
9. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 8, which is characterized in that the seed culture medium
Are as follows: maltose 15g, tryptone 10g, (NH4)2HPO420g, NaCl 10g, yeast extract 5g, pH7.0-7.5, are settled to
1L, shaking flask culture；The fermentation medium is starch 50g, yeast extract 3g, beancake powder 5g, KH2PO42g, MgSO4·
7H2O 0.5g。
10. being used for the microbial-bacterial fertilizer of fruit expanding period as claimed in claim 1 or 2, which is characterized in that the microbial-bacterial fertilizer
The preparation method comprises the following steps:
(1) kitchen garbage is subjected to washing desalination, degreasing, crushed, cross 200 meshes, then high-temperature sterilization under the conditions of 120 DEG C
30-50min is let cool to room temperature, adjusts pH6-7, spare；
(2) saccharomycete bacterium powder, Trichoderma bacterium powder are put into respectively in step (1) smashed kitchen garbage, in 30 DEG C of temperature strips
It ferments under part, fermentation time 3-4d obtains karusen I, spare；
(3) silicate bacteria fermentation liquid and aspergillus oryzae fermentation liquid are put into karusen I, are sent out under the conditions of 30 DEG C of temperature
Ferment adjusts pH6.6-7.4 until stopping fermentation when the viable count in karusen is 5,000,000,000/g, obtains microbial-bacterial fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910223309.XA CN109721385A (en) | 2019-03-22 | 2019-03-22 | A kind of microbial-bacterial fertilizer for fruit expanding period |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910223309.XA CN109721385A (en) | 2019-03-22 | 2019-03-22 | A kind of microbial-bacterial fertilizer for fruit expanding period |
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| CN109721385A true CN109721385A (en) | 2019-05-07 |
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ID=66302704
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107759325A (en) * | 2017-11-03 | 2018-03-06 | 河北益微生物技术有限公司 | A kind of multifunctional biological fertilizer |
| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
| CN109089854A (en) * | 2018-08-03 | 2018-12-28 | 佛山市啊里喜果农业科技有限公司 | A kind of implantation methods of strawberry |
-
2019
- 2019-03-22 CN CN201910223309.XA patent/CN109721385A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107759325A (en) * | 2017-11-03 | 2018-03-06 | 河北益微生物技术有限公司 | A kind of multifunctional biological fertilizer |
| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
| CN109089854A (en) * | 2018-08-03 | 2018-12-28 | 佛山市啊里喜果农业科技有限公司 | A kind of implantation methods of strawberry |
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Application publication date: 20190507 |