CN109702001A - A method of utilizing soil nematodes rehabilitating soil fertility - Google Patents
A method of utilizing soil nematodes rehabilitating soil fertility Download PDFInfo
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- CN109702001A CN109702001A CN201811609968.9A CN201811609968A CN109702001A CN 109702001 A CN109702001 A CN 109702001A CN 201811609968 A CN201811609968 A CN 201811609968A CN 109702001 A CN109702001 A CN 109702001A
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Abstract
The invention belongs to soil fertility restorative procedure fields, and in particular to a method of utilize soil nematodes rehabilitating soil fertility.The following steps are included: several criss-cross deep trench and shallow trench are arranged in interval in area to be repaired；It is sprayed in shallow trench into soil nematodes renovation agent, soil nematodes renovation agent is sufficiently mixed by the component of following parts by weight and is made: 500-800 parts of soil nematodes suspension, 1-3 parts of humic acid, 1-3 parts of ammonium chloride；Apatite is sprinkled into area to be repaired；Dissipation operation is carried out with soil nematodes insecticide；Organic fertilizer is filled in deep trench.Method provided by the invention can effectively degrade organic matter and tenor in soil, can be effectively improved soil environment, rehabilitating soil fertility, application prospect is good, has very high economic benefit and ecological benefits.
Description
Technical field
The invention belongs to soil fertility restorative procedure fields, and in particular to a kind of using soil nematodes rehabilitating soil fertility
Method.
Background technique
Littoral zone be sea-land interaction as it is active, to human activities' reaction and the most sensitive region that globalizes, be
Study the interactive key object of land-sea.Beibu Bay, guangxi area is exactly one of sensitive area comprising the North Sea, is prevented at Qiezhou
Three, city port city.According in the distribution characteristics of (137) Cs and heavy metal " in Beibu Bay, guangxi soil~" research of Hu Jinjun
It has been shown that, has the heavy metal pollutions such as Cd, Cu, this pollution may be by Beibu Bay, guangxi in Beibu Bay, guangxi Soils In The Region
The influence of area's construction of nuclear power station.Long-term farmland is excessively applied fertilizer, marine organisms pollution etc. due to, Beibu Bay, guangxi area soil
There is also have the case where polycyclic aromatic hydrocarbon machine object pollution in earth.Although research shows that only some areas there are slight pollution,
Soil fertility may also be influenced, there are potential ecological risks.Still need to this area's soil carry out fertility reparation.
Currently, soil fertility restorative procedure is varied, Chinese patent CN103058720B, which is disclosed, a kind of utilizes albumen
Enzymatic gold straw degradative enhances the method for soil fertility, and Chinese patent CN105766120B is disclosed a kind of to be applied deeply using stalk
The method that returning to the field is increase soil fertility with crop yield.These above-mentioned methods are all based on the restoration of the ecosystem of bioanalysis, reduce
The use of chemical substance, effectively protects ecological environment.But above two method is essentially consisted in and is built up fertility, and lacks degradation
The method of organic matter in soil, metallic pollution, can not repair Beibu Bay, guangxi Soils In The Region fertility.
Soil nematodes is the total of the one kind such as the root-knot nematode being present in soil, pratylenchus, cyst nematode, Ditylenchus dipsaci
Claim, although a large amount of soil nematodes can harm the crops or vegetable crop, soil nematodes are also organic matter in soil, gold
The important consumption group belonged to.Organic matter in soil, metal Natural Degradation but be unable to do without soil nematodes.If soil can be utilized rationally
Relationship between earth nematode and soil organic matter, metal opens a kind of method using soil nematodes rehabilitating soil fertility, then can be just
Ground materials turn waste into wealth.
To sum up, problem of the existing technology is that lack can degrade PAHs in soil organic matter, metal of one kind dirty
The method of the rehabilitating soil fertility of dye.
Summary of the invention
In order to solve the above technical problem, the present invention provides a kind of method using soil nematodes rehabilitating soil fertility,
Can degrade PAHs in soil content of organics and tenor.
The present invention provides a kind of methods using soil nematodes rehabilitating soil fertility, comprising the following steps:
Step 1, several deep trench, zanjon groove depth 25-40cm, wide 10-25cm is arranged in interval in area to be repaired；It is adjacent
Soil between deep trench is known as level ridges, and edge opens up several shallow trench, shallow ridges perpendicular to deep trench direction interval in level ridges
The deep 10-20cm of slot, wide 10-20cm；
Step 2, it is sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent is by following parts by weight
Component be sufficiently mixed and be made: 500-800 parts of soil nematodes suspension, 1-3 parts of humic acid, 1-3 parts of ammonium chloride；
Naturally 5-14d is placed；
Step 3, it is sprinkled into apatite in area to be repaired, continues to place 5-14d；
Step 4, dissipation operation is carried out with soil nematodes insecticide；
Step 5, lower half storey is filled and led up filled with machine fertilizer, the soil of upper half storey area to be repaired in all deep trench.
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, soil nematodes in soil nematodes suspension
Concentration 10-15 item/mL；The amount of application of soil nematodes renovation agent is 5-10L per acre.
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, the soil nematodes suspension according to
The preparation of lower section method: the complete and full egg capsule of picking, merging fill in the sterilizes culture dish of appropriate amounts of sterilized water, put it into 28 ±
It is cultivated in 1 DEG C of insulating box, the second instar larvae J2 hatched is inoculated into plant root easily susceptible in greenhouse after 3-4d and is carried out
Expand numerous；The egg capsule for expanding numerous acquisition is hatched into second instar larvae J2, be added sterile water be prepared into root-knot nematode suspension 10-15 item/
mL。
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, the soil nematodes be root-knot nematode or
Person's heterorhabditis indica.
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, the amount of application of the apatite is per acre
5-10kg。
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, spacing 1-2m between adjacent deep trenches, phase
Spacing 3-5m between adjacent shallow trench.
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, the soil nematodes renovation agent is used for
Degradation PAHs in soil organic matter and metallic element.
Preferably, in the above-mentioned method using soil nematodes rehabilitating soil fertility, the polycyclic aromatic hydrocarbon organic matter is tricyclic
Luxuriant and rich with fragrance PHE or Fourth Ring pyrene PYR；The metallic element is copper.
Compared with prior art, a kind of method using soil nematodes rehabilitating soil fertility provided by the invention, have with
It is lower the utility model has the advantages that
Soil nematodes renovation agent provided by the invention can effectively degrade organic matter and tenor in soil, pass through test card
It is bright, the experimental group of soil nematodes renovation agent is added after the 14th day, degradable 22.1-26.9% of tricyclic phenanthrene PHE or so, Fourth Ring pyrene
PYR degradable 19.2%, degradable 15.2-18.0% of metallic copper or so.
The present invention is first with the organic matter and tenor in soil nematodes renovation agent degradation soil, pending soil nematodes
Dissipation prevents it from endangering plant growth, finally supplements organic fertilizer, increases soil fertility, and supplementing can be effectively sharp by plant in soil
The substances such as organic matter, amino acid.The use of apatite can make metal-cured in soil, prevent it with water loss, be convenient for
Degradation of the microorganism to metal.By opening up deep trench and shallow trench, by soil nematodes renovation agent and the separated place of organic fertilizer
Reason, nematode in desinsection Shi Kexian emphasis dissipation shallow trench is with strong points, criss-cross groove setting also can be changed water flow to,
Organic matter and metal are fixed in square, play degradation convenient for soil nematodes renovation agent.Organic fertilizer is buried be overlying on it is to be repaired
Under the soil in region, it can prevent nutriment in organic fertilizer from evaporateing into air.
The soil environment in Beibu Bay, guangxi area, rehabilitating soil fertility, application can be effectively improved using method of the invention
Prospect is good, has very high economic benefit and ecological benefits.
Detailed description of the invention
Fig. 1 is the working principle of the invention figure.
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but should not be construed as limitation of the invention.It is following
Experimental method in embodiment is unless otherwise specified conventional method, the materials, reagents and the like used in the following examples, such as
Without specified otherwise, it is commercially available.
The present invention provides a kind of methods using soil nematodes rehabilitating soil fertility, comprising the following steps:
Step 1, several deep trench of parallel interval setting in area to be repaired, zanjon groove depth 25-40cm, wide 10-25cm,
Spacing 1-2m between adjacent deep trenches；Soil between adjacent deep trenches is known as level ridges, and edge is perpendicular to the deep trench in level ridges
Direction is parallel and interval opens up several shallow trench, the deep 10-20cm of shallow trench, wide 10-20cm, spacing 3- between adjacent shallow trench
5m。
Step 2, it is sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent is by following parts by weight
Component be prepared by fully mixing: 500-800 parts of soil nematodes suspension, 1-3 parts of humic acid, 1-3 parts of ammonium chloride；
Naturally 5-7d is placed, with PAHs in soil organic matter of degrading.
Step 3, apatite is sprinkled into area to be repaired, make it is metal-cured prevent its passage in the soil, continue to place
5-7d utilizes metal in soil nematodes degradation soil.
Step 4, dissipation operation is carried out with soil nematodes insecticide, reaching soil nematodes content does not influence plant
Or the normal level of crop growth；
Step 5, lower half storey filled with machine fertilizer, fill and lead up, described by the soil of upper half storey area to be repaired in the deep trench
Organic fertilizer is animal wastes or straw decomposing object.The straw decomposing object is the stalk ripe through EM mycocriny.
The working principle of the invention is as shown in Figure 1.
It should be noted that the soil nematodes that the present invention uses is root-knot nematode or heterorhabditis indica.Following embodiments
In, root-knot nematode is Meloidogyne incognita (Meloidogyne incognita), the Meloidogyne incognita (Meloidogyne
It incognita) is that the serious Tomato Root System of idiopathy, the complete and full egg capsule of picking are acquired by inventor, merging fills suitable
In the sterilizes culture dish for measuring sterile water, puts it into 28 ± 1 DEG C of insulating box and cultivate, the second instar larvae that will be hatched after 3-4d
J2 be inoculated into tomato root easily susceptible in greenhouse expand it is numerous.The egg capsule for expanding numerous acquisition is hatched into two ages children using tray method
Worm J2 is added sterile water and is prepared into root-knot nematode suspension 10-15 item/mL.
In following embodiments, heterorhabditis indica (Heterorhabditis becherriana), the heterorhabditis indica be by
Inventor acquires the serious cucumber root of idiopathy, and different small bar line is made referring to the preparation method of above-mentioned root-knot nematode suspension
Worm suspension 10-15 item/mL.
As long as the embodiment of the present invention is it should be noted that the identified bacterial strain for belonging to soil nematodes can be used in order to grind
Study carefully conveniently, voluntarily draw materials, those skilled in the art also can purchase the bacterial strain of other soil nematodes for testing.
Preferably, the present invention includes following embodiment using the method for soil nematodes rehabilitating soil fertility.
Embodiment 1
A method of utilizing soil nematodes rehabilitating soil fertility, comprising the following steps:
Step 1, several deep trench, zanjon groove depth 25-30cm, wide 10- is arranged in parallel in area to be repaired and interval
15cm, spacing 1m between adjacent deep trenches；Soil between adjacent deep trenches is known as level ridges, and edge is perpendicular to the zanjon in level ridges
Slot direction is parallel and interval opens up several shallow trench, the deep 15-20cm of shallow trench, wide 15-20cm, spacing between adjacent shallow trench
5m。
Step 2, it is sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent is by following parts by weight
Component be sufficiently mixed and be made: 800 parts of soil nematodes suspension, 3 parts of humic acid, 3 parts of ammonium chloride；The soil nematodes is root knot
Nematode, 10/mL of soil nematodes concentration in soil nematodes suspension；The amount of application of soil nematodes renovation agent is 10L per acre.
Naturally 5d is placed, with PAHs in soil organic matter of degrading.
Step 3, be sprinkled into apatite in area to be repaired, amount of application is 5kg per acre, make it is metal-cured in the soil, prevent
Only it is passed, and continues to place 7d, utilizes metal in soil nematodes degradation soil.
Step 4, dissipation operation is carried out with soil nematodes insecticide, reaching soil nematodes content does not influence plant
Or the normal level of crop growth.Soil nematodes insecticide kills ground wire using strength pheretima board.
Step 5, lower half storey filled with machine fertilizer, fill and lead up, described by the soil of upper half storey area to be repaired in all deep trench
Organic fertilizer is animal wastes.
Embodiment 2
A method of utilizing soil nematodes rehabilitating soil fertility, comprising the following steps:
Step 1, several deep trench, zanjon groove depth 35-40cm, wide 20- is arranged in parallel in area to be repaired and interval
25cm, spacing 2m between adjacent deep trenches；Soil between adjacent deep trenches is known as level ridges, and edge is perpendicular to the zanjon in level ridges
Slot direction is parallel and interval opens up several shallow trench, the deep 10-15cm of shallow trench, wide 10-15cm, spacing between adjacent shallow trench
3m。
Step 2, it is sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent is by following parts by weight
Component be sufficiently mixed and be made: 500 parts of soil nematodes suspension, 1 part of humic acid, 1 part of ammonium chloride；The soil nematodes is root knot
Nematode, 15/mL of soil nematodes concentration in soil nematodes suspension；The amount of application of soil nematodes renovation agent is 5L per acre.
Naturally 7d is placed, with PAHs in soil organic matter of degrading.
Step 3, be sprinkled into apatite in area to be repaired, amount of application is 10kg per acre, make it is metal-cured in the soil, prevent
Only it is passed, and continues to place 5d, utilizes metal in soil nematodes degradation soil.
Step 4, dissipation operation is carried out with soil nematodes insecticide, reaching soil nematodes content does not influence plant
Or the normal level of crop growth.Soil nematodes insecticide is clear using the energy board nematode that puts forth energy.
Step 5, lower half storey filled with machine fertilizer, fill and lead up, described by the soil of upper half storey area to be repaired in all deep trench
Organic fertilizer is animal wastes.
Embodiment 3
A method of utilizing soil nematodes rehabilitating soil fertility, comprising the following steps:
Step 1, several deep trench, zanjon groove depth 30-35cm, wide 10- is arranged in parallel in area to be repaired and interval
15cm, spacing 1.5m between adjacent deep trenches；Soil between adjacent deep trenches is known as level ridges, and edge is perpendicular to the depth in level ridges
Groove direction is parallel and interval opens up several shallow trench, the deep 15-20cm of shallow trench, wide 15-20cm, between adjacent shallow trench between
Away from 4m.
Step 2, it is sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent is by following parts by weight
Component be sufficiently mixed and be made: 600 parts of soil nematodes suspension, 2 parts of humic acid, 2 parts of ammonium chloride；The soil nematodes is root knot
Nematode, 12/mL of soil nematodes concentration in soil nematodes suspension；The amount of application of soil nematodes renovation agent is 8L per acre.
Naturally 14d is placed, with PAHs in soil organic matter of degrading.
Step 3, be sprinkled into apatite in area to be repaired, amount of application is 7kg per acre, make it is metal-cured in the soil, prevent
Only it is passed, and continues to place 14d, utilizes metal in soil nematodes degradation soil.
Step 4, dissipation operation is carried out with soil nematodes insecticide, reaching soil nematodes content does not influence plant
Or the normal level of crop growth.Soil nematodes insecticide kills ground wire using strength pheretima board.
Step 5, lower half storey filled with machine fertilizer, fill and lead up, described by the soil of upper half storey area to be repaired in all deep trench
Organic fertilizer is straw decomposing object.The straw decomposing object is the stalk ripe through EM mycocriny.
Embodiment 4
A method of using soil nematodes rehabilitating soil fertility, identical as the operation of embodiment 1, difference is: soil
7/mL of soil nematodes concentration in nematode suspension；The amount of application of soil nematodes renovation agent is 11L per acre；Apatite, amount of application
For 12kg per acre.
In order to verify effect of the invention, We conducted organic matter degradation tests and metal degradation test.
Soil: Beibu Bay, guangxi Qinzhou Prefecture soil is tested, Guangxi Qinzhou section bay belongs to corrosion type in Beibu Bay, guangxi
Seashore, based on silt coast.Test soil is divided into A group, B group, C group, D group, control group.
Tricyclic phenanthrene PHE and copper chloride is manually added in A group, B group, C group, and makes the concentration 100mg/kg of tricyclic phenanthrene PHE
Soil, the concentration of copper ion are 50mg/kg soil；A group, B group, component C not Jia Ru soil-repairing agent in embodiment 1-3, every thousand
Gram be added 1mL.
Fourth Ring pyrene PYR and copper chloride is manually added in D group, and makes the concentration 100mg/kg soil of Fourth Ring pyrene PYR, copper ion
Concentration be 50mg/kg soil；Then soil-repairing agent in embodiment 1, every kilogram of addition 1mL is added.
Control group is placed naturally, without any processing.
Above-mentioned four groups are placed 14 days naturally, test organic matter and copper coin cellulose content in the 7th day and the 14th day respectively, as a result such as
Under:
A group tricyclic phenanthrene PHE containing flow control 7 days be 86.5mg/kg soil, be 73.1mg/kg soil within the 14th day, copper ion contains
Flow control 7 days be 43.5mg/kg soil, be 42.0mg/kg soil within the 14th day.
B group tricyclic phenanthrene PHE containing flow control 7 days be 85.1mg/kg soil, be 77.9mg/kg soil within the 14th day, copper ion contains
Flow control 7 days be 43.5mg/kg soil, be 41.1mg/kg soil within the 14th day.
C group tricyclic phenanthrene PHE containing flow control 7 days be 85.2mg/kg soil, be 74.1mg/kg soil within the 14th day, copper ion contains
Flow control 7 days be 43.7mg/kg soil, be 42.3mg/kg soil within the 14th day.
D group Fourth Ring pyrene PYR containing flow control 7 days be 88.5mg/kg soil, be 81.8mg/kg soil within the 14th day, copper ion contains
Flow control 7 days be 44.6mg/kg soil, be 41.0mg/kg soil within the 14th day.
Control group tricyclic phenanthrene PHE containing being within flow control 7 days 98.3mg/kg soil, be within the 14th day 90.4mg/kg soil, copper from
Son containing flow control 7 days be 48.6mg/kg soil, be 47.7mg/kg soil within the 14th day.
The above results show that can effectively degrade organic matter and metal in soil of soil nematodes renovation agent provided by the invention contains
Amount.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of method using soil nematodes rehabilitating soil fertility, which comprises the following steps:
Step 1, several deep trench, zanjon groove depth 25-40cm, wide 10-25cm is arranged in interval in area to be repaired；Adjacent zanjon
Soil between slot is known as level ridges, and edge opens up several shallow trench perpendicular to deep trench direction interval in level ridges, shallow trench
Deep 10-20cm, wide 10-20cm；
Step 2, sprayed in the shallow trench into soil nematodes renovation agent, the soil nematodes renovation agent by following parts by weight group
Divide to be sufficiently mixed and be made: 500-800 parts of soil nematodes suspension, 1-3 parts of humic acid, 1-3 parts of ammonium chloride；
Naturally 5-14d is placed；
Step 3, it is sprinkled into apatite in area to be repaired, continues to place 5-14d；
Step 4, dissipation operation is carried out with soil nematodes insecticide；
Step 5, lower half storey is filled and led up filled with machine fertilizer, the soil of upper half storey area to be repaired in all deep trench.
2. the method according to claim 1 using soil nematodes rehabilitating soil fertility, which is characterized in that soil nematodes is outstanding
Soil nematodes concentration 10-15 item/mL in supernatant liquid；The amount of application of soil nematodes renovation agent is 5-10L per acre.
3. the method according to claim 2 using soil nematodes rehabilitating soil fertility, which is characterized in that the soil line
Worm suspension is prepared in accordance with the following methods: the complete and full egg capsule of picking, and merging fills the sterilizes culture dish of appropriate amounts of sterilized water
In, put it into 28 ± 1 DEG C of insulating box and cultivate, after 3-4d by the second instar larvae J2 hatched be inoculated into greenhouse easily it is susceptible
Plant root expand it is numerous；The egg capsule for expanding numerous acquisition is hatched into second instar larvae J2, sterile water is added and is prepared into root-knot nematode
Suspension 10-15 item/mL.
4. the method according to claim 2 using soil nematodes rehabilitating soil fertility, which is characterized in that the soil line
Worm is root-knot nematode or heterorhabditis indica.
5. the method according to claim 1 using soil nematodes rehabilitating soil fertility, which is characterized in that the apatite
Amount of application be 5-10kg per acre.
6. the method according to claim 1 using soil nematodes rehabilitating soil fertility, which is characterized in that adjacent deep trenches
Between spacing 1-2m, spacing 3-5m between adjacent shallow trench.
7. the method according to claim 1 using soil nematodes rehabilitating soil fertility, which is characterized in that by the soil
Nematode renovation agent is for degrade PAHs in soil organic matter and metallic element.
8. the method according to claim 1 using soil nematodes rehabilitating soil fertility, which is characterized in that the polycyclic virtue
Hydrocarbon organic matter is tricyclic phenanthrene PHE or Fourth Ring pyrene PYR；The metallic element is copper.
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| CN201811609968.9A CN109702001B (en) | 2018-12-27 | 2018-12-27 | Method for restoring soil fertility by utilizing soil nematodes |
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| CN110012690A (en) * | 2019-05-20 | 2019-07-16 | 成都市农林科学院 | A kind of acidification soil improvement method suitable for tea place |
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| Publication number | Publication date |
|---|---|
| CN109702001B (en) | 2020-12-29 |
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