CN109601532A - The anti-target slow-release nano-pesticide formulation and the preparation method and application thereof to eliminate aphis - Google Patents
The anti-target slow-release nano-pesticide formulation and the preparation method and application thereof to eliminate aphis Download PDFInfo
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Abstract
The present invention provides a kind of anti-target slow-release nano-pesticide formulation and preparation method thereof to eliminate aphis, belongs to technical field of pesticide preparations.Using Tripolyglycerol monostearates, P (HB-HO), prevent eliminating aphis pesticide, PVA and aphid sex pheromone as raw material；It is successively dissolved in organic phase according to Tripolyglycerol monostearates, P (HB-HO) and the anti-pesticide that eliminates aphis；PVA is dissolved into certain density PVA aqueous solution, is mixed with organic, it is high-pressure homogeneous, lotion is formed, being sufficiently stirred makes organic phase volatilize, lotion high speed centrifugation, abandons supernatant, collects sediment；Aphid sex pheromone is dissolved in dehydrated alcohol, is mixed with the sediment of collection, dipping absorption, high speed centrifugation collects sediment, is freeze-dried, and is made.Invention formulation particle diameter distribution is uniform, good dispersion degree, with good sustained release performance, have the function of that active targeting is lured and killed to aphid, the drug effect of pesticide can be improved, reduce pesticide again to the pollution of environment and the harm of non-target organism, achieve the purpose that actively to lure target pest and is killed.
Description
Technical field
The invention belongs to technical field of pesticide preparations, and in particular to a kind of anti-target slow-release nano-pesticide formulation to eliminate aphis
And the preparation method and application thereof.
Background technique
Aphid is under the jurisdiction of Homoptera Aphidoidea, is that reproduction speed is most fast in the world and one of most destructive insect.Aphid
The harmfulness of worm is very strong, and extremely strong about 250 kinds destructive to agriculture, woods and horticulture.It is about 1100 kinds of aphid, main in China
Grain and industrial crops are endangered by feeding and transmitted virus, different degrees of break can be caused to the yield and quality of crops
It is bad.For a long time, people are dedicated to prevention and treatment to aphid with the methods of physical measure using spraying insecticide, wherein using chemical agriculture
It is one of most important means that medicine, which carries out prevention and treatment,.The use of pesticide is that the prevention and treatment of aphid mycoses and the stable and high yields of agricultural product mention
Strong guarantee has been supplied, has brought huge interests to the mankind.However, pesticide used at present is still with missible oil, wettable
Based on the conventional dosage forms such as pulvis, pulvis, suspending agent.These dosage forms are during the preparation process, largely living using organic solvent and surface
Property agent；Due to wind, the influence for the external environments such as drench with rain in use process, quite a few pesticide residue is in agricultural product or dispersion
In big gas and water and soil, a large amount of pesticides is caused to be lost, while to people and animals, insect etc. without selectivity, only small part
(20%-30%) can act on crop target site and action time is short.In order to achieve the desired results, it has to increase application
Number and dosage cause agriculture very serious residual and problem of environmental pollution, and directly threaten human health.This has become agriculture
Medicine and environmental area urgent problem to be solved.
It is to solve problem above that controlled release, slow-release, which especially have efficient, less toxic environment-friendly type targeting sustained and controlled release pesticide,
Important means.It is quickly grown in recent years, pesticide delays controlled-release technology, makes macromolecule chemical combination using means physically or chemically
Object carrier and pesticide interact, and continue pesticide according to required dosage, stable release, are most passed through with reaching
Ji safely, effectively controls harmful organism.Compared with conventional dosage forms, pesticide slow-releasing agent, which has a characteristic that, changes pesticide
Existing forms, reduce the influence of the environmental elements such as big gas and water, convenient for storage and use；The effective concentration that ensure that pesticide,
Being widely varied for drug concentration in target can be solved in a certain range, improve bioavailability；Long-acting, slow can discharge,
Using pesticide, reduce dosage and times for spraying, the probability of problem of environmental pollution generation can be reduced.Have and prevents the chemistry that eliminates aphis
Slow-releasing pesticide preparation related patents and document report (patent: CN102823601A pymetrozine slow-release granule and its preparation and
Using the micro- preparation method by capsule of CN105519562A imidacloprid；Document: the preparation of Zhu Yingfei gaultherolin sustained-release microspheres and
Its ecological functions evaluation [D] Shandong Agricultural University, 2015；) but the chemical pesticide sustained release that is prepared in current patent and paper
Preparation all lacks to the specific active targeting of aphid mostly, does not reduce the harm to non-target organism.Therefore, one kind is developed
It has important practical significance for the target slow-release nano-pesticide formulation of aphid.
Summary of the invention
The purpose of the present invention is being directed to the deficiency for lacking active targeting existing for above-mentioned existing chemical pesticide sustained release preparation,
A kind of anti-target slow-release nano-pesticide formulation and preparation method thereof to eliminate aphis is provided.
To achieve the above object, the technical scheme adopted by the invention is that: a kind of anti-target slow-release nanometer agriculture to eliminate aphis
Medicine preparation, in which: using the matrix of following parts by weight as raw material:
5~50 parts of Tripolyglycerol monostearates, 20~100 parts of P (HB-HO), anti-eliminate aphis 10~50 parts of pesticide, PVA1
~20 parts, 5~10 parts of aphid sex pheromone；
The method for preparing the anti-target slow-release nano-pesticide formulation to eliminate aphis, in which: the following steps are included:
The following steps are included:
(1) by the anti-agriculture that eliminates aphis of 5~50 parts of Tripolyglycerol monostearates, 20~100 parts of P (HB-HO) and 10~50 parts
Medicine is successively dissolved in organic phase, and the Tripolyglycerol monostearates, P (HB-HO) and anti-eliminates aphis pesticide in organic phase
Concentration be respectively 0.5~5%, 2~10% and 1~5%；
(2) 1~20 part of PVA is taken, the PVA aqueous solution that concentration is 0.1~2% is dissolved into, with organic phase made from step (1)
After being mixed, 10min is stirred under the revolving speed of 1000rpm, then high pressure is equal under conditions of 500-1000bar at room temperature
Matter 2-6 circulations, form lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) 5~10 parts of aphid sex pheromones is taken to be dissolved in dehydrated alcohol, after being mixed with the sediment that step (3) are collected, leaching
Stain adsorbs 30min, and 10min is centrifuged under the revolving speed of 15000rpm, abandons supernatant, collects sediment, and freeze-drying is targeted
P (HB-HO) slow release nanometer pesticidal preparations.
Further preferred scheme, the organic phase are chloroform or methylene chloride.
Further preferred scheme, the anti-pesticide that eliminates aphis is appointing in pymetrozine, imidacloprid, Acetamiprid or avermectin
It anticipates one kind.
Further preferred scheme, the aphid sex pheromone are the mixture of nepetalactone and nepetol, the two mass ratio
For 1~3:1~3.
Further preferred scheme, the P (HB-HO) are hydroxybutyric acid and Hydroxyoctanoic acid copolymer.
Further preferred scheme, the PVA are polyvinyl alcohol.
Target slow-release nano-pesticide formulation of the present invention is applied in targeting prevention and treatment aphid mycoses.
Compared with prior art, it has the advantage that
First, carrier of the present invention using P (HB-HO) as pesticide, material is easy to get, and cost is relatively low, degradable.
Second, the present invention is prepared for targeting P (HB-HO) slow release nanometer pesticidal preparations, the system using high-pressure homogeneous-absorption method
Agent particle diameter distribution is uniform, good dispersion degree, has good sustained release performance.
Third, the double action that target slow-release pesticidal preparations of the invention there is active targeting to lure and kill aphid,
The drug effect and pesticide that pesticide can be improved reach to the pollution of environment and the harm of non-target organism and actively lure target
Mark pest and the purpose killed.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture for targeting P (HB-HO) and carrying pymetrozine slow release nanometer formulation.
Fig. 2 is the grain size distribution for targeting P (HB-HO) and carrying pymetrozine slow release nanometer formulation.
Specific embodiment
Embodiment 1:
The target slow-release nano-pesticide formulation to eliminate aphis is prevented described in the present embodiment, in which: with the matrix of following parts by weight
For raw material:
5 parts of Tripolyglycerol monostearates, 20 parts of P (HB-HO), 10 parts of pymetrozine, PVA1 parts, aphid sex pheromone 10
Part；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and pymetrozine are successively dissolved in dichloromethane
In alkane, and Tripolyglycerol monostearates, P (HB-HO) and the concentration of pymetrozine in methylene chloride is respectively
0.5%, 2% and 1%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 0.1%, has with made from step (1)
After machine is mutually mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 6 under conditions of 500bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 1:3 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min under the revolving speed of 15000rpm,
Supernatant is abandoned, sediment is collected, freeze-drying obtains targeting P (HB-HO) and carries pymetrozine slow release nanometer formulation.
Pymetrozine slow release nanometer formulation is carried to above-mentioned targeting P (HB-HO) and is characterized (such as Fig. 1 transmission electron microscope picture, Fig. 2 grain
Diameter distribution map), the results showed that said preparation is spherical, good dispersion degree, and particle diameter distribution is in 150-260nm or so, drugloading rate and encapsulating
Rate is respectively 25.32%, 75.96%, and the lower 30 days cumulative release rates of laboratory condition are up to 94.21%.
It takes the arabidopsis of 20 plants of indoor cultures to be equally divided into 2 groups, targeting P (HB-HO) is carried into pymetrozine slow release nanometer formulation
Suspension is sprayed on first group wherein on the blade of 1 plant of arabidopsis, and it is mixed that non-targeted P (HB-HO) carries pymetrozine slow release nanometer formulation
Suspension (be free of aphid sex pheromone) is sprayed on second group wherein on the blade of 1 plant of arabidopsis, 100 aphids of every group of dispensing, 7d
After observed and calculate every group of aphid Mean Death quantity (every group of test is in triplicate).The result shows that: the 1st group and the 2nd group
Aphid Mean Death quantity be respectively 78 ± 5.1,25 ± 3, prepared targeting P (HB-HO) carries pymetrozine sustained release and receives
Metric system agent is lured with good active targeting aphid and toxic action.
Embodiment 2:
The target slow-release nano-pesticide formulation to eliminate aphis is prevented described in the present embodiment, in which: with the matrix of following parts by weight
For raw material:
45 parts of Tripolyglycerol monostearates, 100 parts of P (HB-HO), 50 parts of imidacloprid, PVA10 parts, aphid sex pheromone 7
Part；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and imidacloprid are successively dissolved in dichloromethane
In alkane, and Tripolyglycerol monostearates, P (HB-HO) and the concentration of imidacloprid in methylene chloride is respectively
4.5%, 10% and 5%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 1%, and organic made from step (1)
After mutually being mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 2 under conditions of 1000bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 3:1 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min at revolving speed 15000rpm, abandons
Supernatant, collects sediment, and freeze-drying obtains targeting P (HB-HO) and carries imidacloprid slow release nanometer formulation.
To above-mentioned targeting P (HB-HO) carry imidacloprid slow release nanometer formulation characterize, the results showed that said preparation be it is spherical,
Good dispersion degree, particle diameter distribution is in 250-340nm or so, and drugloading rate and encapsulation rate are respectively 29.01%, 87.03%, laboratory item
The lower 30 days cumulative release rates of part are up to 90.73%.
It takes the arabidopsis of 20 plants of indoor cultures to be equally divided into 2 groups, targeting P (HB-HO) is carried into imidacloprid slow release nanometer formulation
Suspension is sprayed on first group wherein on the blade of 1 plant of arabidopsis, and it is mixed that non-targeted P (HB-HO) carries imidacloprid slow release nanometer formulation
Suspension (be free of aphid sex pheromone) is sprayed on second group wherein on the blade of 1 plant of arabidopsis, 100 aphids of every group of dispensing, 7d
After observed and calculate aphid Mean Death quantity (every group of test is in triplicate).The result shows that: the 1st group and the 2nd group of aphid
Worm Mean Death quantity is respectively 89 ± 6.3,30 ± 5, and prepared targeting P (HB-HO) carries imidacloprid slow release nanometer system
Agent is lured with good active targeting aphid and toxic action.
Embodiment 3:
The target slow-release nano-pesticide formulation to eliminate aphis is prevented described in the present embodiment, in which: with the matrix of following parts by weight
For raw material:
30 parts of Tripolyglycerol monostearates, 30 parts of P (HB-HO), 15 parts of Acetamiprid, PVA20 parts, aphid sex pheromone 5
Part；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and Acetamiprid are successively dissolved in dichloromethane
In alkane, and Tripolyglycerol monostearates, P (HB-HO) and the concentration of Acetamiprid in methylene chloride be respectively 3%,
3% and 1.5%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 2%, and organic made from step (1)
After mutually being mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 5 under conditions of 600bar
Circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 1:1 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min under the revolving speed of 15000rpm,
Supernatant is abandoned, sediment is collected, freeze-drying obtains targeting P (HB-HO) and carries Acetamiprid slow release nanometer pesticidal preparations.
It carries Acetamiprid slow release nanometer pesticidal preparations to above-mentioned targeting P (HB-HO) to characterize, the results showed that said preparation is
Spherical, good dispersion degree, for particle diameter distribution in 270-390nm or so, drugloading rate and encapsulation rate are respectively 30.24%, 90.72%, real
30 days cumulative release rates are up to 90.55% under the conditions of testing room.
It takes the arabidopsis of 20 plants of indoor cultures to be equally divided into 2 groups, targeting P (HB-HO) is carried into Acetamiprid slow release nanometer formulation
Suspension is sprayed on first group wherein on the blade of 1 plant of arabidopsis, and it is mixed that non-targeted P (HB-HO) carries Acetamiprid slow release nanometer formulation
Suspension (be free of aphid sex pheromone) is sprayed on second group wherein on the blade of 1 plant of arabidopsis, 100 aphids of every group of dispensing, 7d
After observed and calculate aphid Mean Death quantity (every group of test is in triplicate).The result shows that: the 1st group and the 2nd group of aphid
Worm Mean Death quantity is respectively 86 ± 4.7,28 ± 3.9, and prepared targeting P (HB-HO) carries Acetamiprid slow release nanometer
Preparation is lured with good active targeting aphid and toxic action.
Embodiment 4:
The anti-target slow-release nano-pesticide formulation to eliminate aphis of the present embodiment, in which: with the matrix of following parts by weight be original
Material:
10 parts of Tripolyglycerol monostearates, 80 parts of P (HB-HO), 20 parts of avermectin, PVA15 parts, aphid sex pheromone
6 parts；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and avermectin are successively dissolved in trichlorine
In methane, and the Tripolyglycerol monostearates, the concentration of P (HB-HO) and avermectin in chloroform are respectively
1%, 8% and 2%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 1.5%, has with made from step (1)
After machine is mutually mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 4 under conditions of 700bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 1:2 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min under the revolving speed of 15000rpm,
Supernatant is abandoned, sediment is collected, freeze-drying obtains targeting P (HB-HO) and carries avermectin slow release nanometer pesticidal preparations.
It carries avermectin slow release nanometer pesticidal preparations to above-mentioned targeting P (HB-HO) to characterize, the results showed that said preparation
To be spherical, good dispersion degree, for particle diameter distribution in 150-260nm or so, drugloading rate and encapsulation rate are respectively 16.85%, 84.25%,
The lower 30 days cumulative release rates of laboratory condition are up to 92.45%.
It takes the arabidopsis of 20 plants of indoor cultures to be equally divided into 2 groups, targeting P (HB-HO) is carried into avermectin slow release nanometer system
Agent suspension is sprayed on first group wherein on the blade of 1 plant of arabidopsis, and non-targeted P (HB-HO) carries avermectin slow release nanometer system
Agent suspension (be free of aphid sex pheromone) is sprayed on second group wherein on the blade of 1 plant of arabidopsis, 100 aphids of every group of dispensing
Worm is observed after 7d and calculates aphid Mean Death quantity (every group of test is in triplicate).The result shows that: the 1st group and the 2nd group
Aphid Mean Death quantity be respectively 72 ± 8.2,22 ± 4, prepared targeting P (HB-HO) carries avermectin sustained release
Nanometer formulation is lured with good active targeting aphid and toxic action.
Embodiment 5:
The anti-target slow-release nano-pesticide formulation to eliminate aphis of the present embodiment, in which: with the matrix of following parts by weight be original
Material:
50 parts of Tripolyglycerol monostearates, 60 parts of P (HB-HO), 30 parts of imidacloprid, PVA8 parts, aphid sex pheromone 8
Part；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and imidacloprid are successively dissolved in three chloromethanes
In alkane, and the Tripolyglycerol monostearates, the concentration of P (HB-HO) and imidacloprid in chloroform be respectively 5%,
6% and 3%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 0.8%, has with made from step (1)
After machine is mutually mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 3 under conditions of 800bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 2:1 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min under the revolving speed of 15000rpm,
Supernatant is abandoned, sediment is collected, freeze-drying obtains targeting P (HB-HO) and carries imidacloprid slow release nanometer pesticidal preparations.
It carries imidacloprid slow release nanometer pesticidal preparations to above-mentioned targeting P (HB-HO) to characterize, the results showed that said preparation is
Spherical, good dispersion degree, for particle diameter distribution in 205-280nm or so, drugloading rate and encapsulation rate are respectively 27.46%, 82.38%, real
30 days cumulative release rates are up to 93.01% under the conditions of testing room.
Embodiment 6:
The anti-target slow-release nano-pesticide formulation to eliminate aphis of the present embodiment, in which: with the matrix of following parts by weight be original
Material:
20 parts of Tripolyglycerol monostearates, 40 parts of P (HB-HO), 40 parts of Acetamiprid, PVA18 parts, aphid sex pheromone 9
Part；
The P (HB-HO) is hydroxybutyric acid and Hydroxyoctanoic acid copolymer；The PVA is polyvinyl alcohol.
The specific preparation method of the above-mentioned anti-target slow-release nano-pesticide formulation to eliminate aphis, comprising the following steps:
(1) Tripolyglycerol monostearates of above-mentioned parts by weight, P (HB-HO) and Acetamiprid are successively dissolved in three chloromethanes
In alkane, and the Tripolyglycerol monostearates, the concentration of P (HB-HO) and Acetamiprid in chloroform be respectively 2%,
4% and 4%；
(2) PVA for taking above-mentioned parts by weight is dissolved into the PVA aqueous solution that concentration is 1.8%, has with made from step (1)
After machine is mutually mixed, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 3 under conditions of 900bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), so that organic phase is volatilized completely, makes lotion 15000rpm's
It is centrifuged 10min under revolving speed, abandons supernatant, collects sediment；
(4) aphid sex pheromone (nepetalactone is 1:1 with nepetol mass ratio) of above-mentioned parts by weight is taken to be dissolved in anhydrous
In ethyl alcohol, after mixing with the sediment that step (3) are collected, dipping absorption 30min is centrifuged 10min under the revolving speed of 15000rpm,
Supernatant is abandoned, sediment is collected, freeze-drying obtains targeting P (HB-HO) and carries Acetamiprid slow release nanometer pesticidal preparations.
It carries Acetamiprid slow release nanometer pesticidal preparations to above-mentioned targeting P (HB-HO) to characterize, the results showed that said preparation is
Spherical, good dispersion degree, for particle diameter distribution in 160-275nm or so, drugloading rate and encapsulation rate are respectively 39.38%, 78.76%, real
30 days cumulative release rates are up to 93.43% under the conditions of testing room.
Claims (10)
1. a kind of anti-target slow-release nano-pesticide formulation to eliminate aphis, it is characterised in that: with the matrix of following parts by weight be original
Material:
5~50 parts of Tripolyglycerol monostearates, 20~100 parts of P (HB-HO), anti-eliminate aphis 10~50 parts of pesticide, PVA1~20
Part, 5~10 parts of aphid sex pheromone.
2. a kind of method for preparing the target slow-release nano-pesticide formulation for preventing eliminating aphis described in claim 1, which is characterized in that packet
Include following steps:
(1) by 5~50 parts of Tripolyglycerol monostearates, 20~100 parts of P (HB-HO) and 10~50 parts it is anti-eliminate aphis pesticide according to
It is secondary to be dissolved in organic phase, and the Tripolyglycerol monostearates, P (HB-HO) and the anti-pesticide that eliminates aphis are dense in organic phase
Degree is respectively 0.5~5%, 2~10% and 1~5%；
(2) 1~20 part of PVA is taken, the PVA aqueous solution that concentration is 0.1~2% is dissolved into, is carried out with organic phase made from step (1)
After mixing, 10min is stirred under the revolving speed of 1000rpm, then at room temperature high-pressure homogeneous 2-6 under conditions of 500-1000bar
A circulation forms lotion；
(3) 8h is sufficiently stirred in lotion made from step (2), organic phase is made to volatilize completely, make lotion in the revolving speed of 15000rpm
Lower centrifugation 10min abandons supernatant, collects sediment；
(4) 5~10 parts of aphid sex pheromones are taken to be dissolved in dehydrated alcohol, after mixing with the sediment that step (3) are collected, dipping is inhaled
Attached 30min is centrifuged 10min under the revolving speed of 15000rpm, abandons supernatant, collects sediment, and freeze-drying obtains targeting P
(HB-HO) slow release nanometer pesticidal preparations.
3. preventing the preparation method of the target slow-release nano-pesticide formulation to eliminate aphis according to claim 2, it is characterised in that: institute
The organic phase stated is chloroform or methylene chloride.
4. preventing the target slow-release nano-pesticide formulation to eliminate aphis according to claim 1, it is characterised in that: the prevention and treatment aphid
Worm pesticide is any one in pymetrozine, imidacloprid, Acetamiprid or avermectin.
5. preventing the target slow-release nano-pesticide formulation to eliminate aphis according to claim 1, it is characterised in that: the aphid property
Pheromones are the mixture of nepetalactone and nepetol, and the two mass ratio is 1~3:1~3.
6. according to claim 1 or the 5 anti-target slow-release nano-pesticide formulations to eliminate aphis, it is characterised in that: the aphid
Worm sex pheromone is the mixture of nepetalactone and nepetol, and the two mass ratio is 1~3:1~3.
7. preventing the target slow-release nano-pesticide formulation to eliminate aphis according to claim 1, it is characterised in that: the P (HB-HO)
For hydroxybutyric acid and Hydroxyoctanoic acid copolymer.
8. preventing the target slow-release nano-pesticide formulation that eliminates aphis according to claim 1, it is characterised in that: the PVA is
Polyvinyl alcohol.
9. preventing the preparation method of the target slow-release nano-pesticide formulation to eliminate aphis according to claim 2, it is characterised in that: institute
The anti-pesticide that eliminates aphis stated is any one in pymetrozine, imidacloprid, Acetamiprid or avermectin.
10. according to claim 1, preventing the target slow-release nano-pesticide formulation to eliminate aphis, feature described in any one of 4,5,7,8
It is: application of the target slow-release nano-pesticide formulation in targeting prevention and treatment aphid mycoses.
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