CN109588376B - Silkworm egg preservation method - Google Patents
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a silkworm egg preservation method, and particularly relates to the technical field of insect breeding. The invention comprises the following steps: a. protection of silkworm eggs: placing silkworm eggs on different silkworm trays according to the overyear egg and the non-overyear egg, and protecting for 55-65 hours; b. disinfecting silkworm eggs: spraying or soaking with disinfectant at 12-20 deg.C; c. activating silkworm eggs to obtain activated eggs: soaking in activating solution at 6-12 deg.C for 12-28 min; d. after the activated eggs are protected, the eggs are placed in a cold storage for storage: spraying the protective solution at the temperature of 1-5 ℃, airing, and storing in a refrigeration house at the temperature of-6 to-12 ℃. The silkworm eggs have long preservation time, uniform incubation and high daily hatching rate. The method is simple to operate, flexible and controllable, and easy to industrialize.
Description
[ technical field ] A method for producing a semiconductor device
The invention relates to the technical field of insect breeding, in particular to a method for preserving silkworm eggs.
[ background of the invention ]
Silkworm is the larva of silkworm moth, the main source of silk raw material, and plays an important role in human economic life and cultural history. The silkworm breeding and silk taking are great inventions of ancient workers in China, the silkworm career is continuously developed nowadays, the area of a mulberry field is continuously enlarged, and the number of the silkworms bred is continuously increased.
In order to adapt to the change of natural environment, silkworms are differentiated into two constitutional characteristics of generating overyear eggs or diapause eggs in a severe environment and generating non-overyear eggs or diapause eggs in a mild environment after long evolution. The overyear eggs can not be stored for a long time after being laid out and need to be incubated in a short time, and the overyear eggs can be stored at a low temperature for nearly 1 year and incubated after being stimulated and activated under severe conditions.
At present, for the non-overyear eggs, in order to protect the silkworm seed resources, the continuous breeding and feeding can be continued only through continuous breeding for a long time, a large amount of manpower and material resources are consumed, and the hatching time can not be reasonably arranged according to a seed plan and a winter mulberry dormancy period. For silkworm eggs of overyear egg variety, different low-temperature refrigeration methods are adopted in advance according to the planned seed using time, so that the silkworm eggs can reach the hatchable level in the planned seed using time, and the preservation method is complicated and lack of flexibility. For the preservation of silkworm eggs, chinese patent publication No. CN106508827A entitled a method for reducing the number of breeding generations of non-diapause diversified silkworm germplasm resources and patent documents of application disclose a method for low-temperature refrigeration of non-overyear eggs, which can preserve silkworm eggs for 25-28 days, just avoid the resting period of mulberry trees, provide convenience for production and life, but the preservation time is short, the flexibility is poor, and the incubation rate is low if the preservation time is too long. In the patent document of Chinese patent No. CN1123289C entitled method for slightly freezing and preserving silkworm eggs, a staged low-temperature refrigeration and slight freezing method is adopted according to different silkworm breeding seed times, the requirement of using seeds at any time from the current year to the spring of the second year is solved, but the preservation temperature is continuously changed according to the seed using plan to realize the planned incubation of the silkworm eggs, and the slight freezing temperature is-0.5 to-5.5 ℃, the temperature is extremely low, part of the silkworm eggs are difficult to bear, so that the incubation rate is unstable when the silkworm eggs are used in spring, and the one-day incubation rate is low.
Therefore, it is necessary to provide a method for preserving silkworm eggs, which solves the problems of the method for preserving overyear eggs in and without overyear eggs, such as multiple steps, complicated operation, low flexibility, unstable hatching rate and low daily hatching rate.
[ summary of the invention ]
The invention aims to: aiming at the problems of multiple steps, complicated operation, low flexibility, unstable hatchability and low daily hatchability of the method for preserving overyear eggs, the method for preserving the silkworm eggs is provided. The silkworm eggs have long preservation time, uniform incubation and high daily hatching rate. The method is simple to operate, flexible and controllable, and easy to industrialize.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear egg and the non-overyear egg, and placing the silkworm eggs in a silkworm rearing room for development protection for 55-65 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 22-26 ℃ and the humidity of 70-74%, protecting for 46-52 hours, then adjusting the temperature of the silkworm rearing room to 14-18 ℃, and continuing to protect for 9-17 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing the overyear eggs at 20-25 ℃ and the humidity of 76% -82%, and protecting to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing ovum A and the developing ovum B with a disinfectant at the temperature of 12-20 ℃ to obtain disinfected ovum;
c. activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 6-12 ℃ and the humidity to 30% -40%, then taking out the sterilized eggs, soaking the sterilized eggs in an activating solution at 6-12 ℃ for 12-28 minutes, and then taking out the sterilized eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 80-140 parts of phosphate buffer solution, 5-15 parts of mulberry leaf, 6-12 parts of Turkey red oil, 6-10 parts of ethyl acetate, 4-10 parts of orange peel, 2-7 parts of bamboo leaf, 1-5 parts of watermelon peel, 802-4 parts of tween and 1-3 parts of trehalose; the pH value of the phosphate buffer solution is 5.0-7.0;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying the activated eggs with a protective solution at 1-5 ℃ against the activated eggs, spraying the protective solution for 1 time again after the protective solution on the surfaces of the activated eggs is dried in the air, drying the protective solution on the surfaces, and then storing the protective solution in a refrigeration house at-6 to-12 ℃; the protective solution comprises the following raw materials in parts by weight: 6-12 parts of sorbitol, 4-12 parts of glycerol, 3-9 parts of egg white, 4-8 parts of chitosan, 2-8 parts of cyclodextrin, 1-7 parts of trehalose and 0.4-0.8 part of ethyl acetate;
preferably, the preparation method of the activating solution in the step c comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution to soak for 50-70 minutes after crushing, then carrying out reflux extraction for 70-90 minutes at 50-60 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 8-16 minutes, setting the ultrasonic temperature to be 25-35 ℃, the ultrasonic power to be 150-170W, adding trehalose, and uniformly stirring to obtain an activation solution;
preferably, the preparation method of the protective solution in the step d comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; taking trehalose, adding ethyl acetate at 4-10 ℃, stirring and wetting, then continuously keeping the temperature at 4-10 ℃, adding cyclodextrin, uniformly mixing, then stirring at 40-50 ℃ until no ethyl acetate smell exists, then adding egg white, carrying out ultrasonic treatment at 20-30 ℃ for 5-10 minutes, then adding chitosan, continuously carrying out ultrasonic treatment at 20-30 ℃ for 15-25 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Preferably, the disinfectant in the step b comprises the following raw materials in parts by weight: 3-15 parts of folium artemisiae argyi, 3-11 parts of sweet wormwood herb, 2-8 parts of houttuynia cordata, 0.3-0.7 part of snake oil, 0.1-0.5 part of eucalyptus oil, 35-65 parts of acetone and 60-100 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 50-60%.
Still further preferably, the preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 62-72 ℃ for 20-40 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 35-45 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
Still further preferably, the use method of the disinfectant comprises the following steps: spraying the solution with volume of 3-7 ml/piece of silkworm egg.
Still further preferably, the use method of the disinfectant comprises the following steps: soaking silkworm egg and silkworm basket in disinfectant for 4-10 min.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the invention adopts a preservation method, simultaneously preserves the biennial eggs and the non-biennial eggs, and adopts different temperature and humidity conditions to protect the biennial eggs and the non-biennial eggs; then, stimulating the silkworm eggs by using the activating solution to provide a stable energy source for the development of the silkworm eggs; then the silkworm eggs are protected by the protective solution to prevent freezing and then stored at the low temperature of-6 to-12 ℃ to make the silkworm eggs which grow earlier slow or stop, and the eggs which grow slowly continue to grow at the low temperature, thus promoting the uniform hatching. The method of the invention has the advantages that the silkworm eggs are hatched uniformly, and the hatching rate is stabilized to more than 90% per day; the silkworm eggs have long storage time, wherein the hatching rate of the silkworm eggs in the non-overyear period is still more than 90% per day after the silkworm eggs are stored for 12 months, and the hatching rate of the silkworm eggs in the overyear period is still more than 92% per day after the silkworm eggs are stored for 18 months. The method is simple to operate, flexible and controllable, and easy to industrialize.
2. The invention firstly activates the silkworm eggs, then carries out anti-freezing protection and then carries out low-temperature preservation. The activation before low-temperature preservation can stimulate silkworm eggs, and the activity of various biological enzymes in the silkworm egg development process is regulated to orderly change according to the egg growth rule through superoxide dismutase, catalase and various nutritional ingredients extracted from mulberry leaves, orange peels, bamboo leaves and watermelons in the activation solution, so that the activity of the two key biological enzymes can be enhanced, and the growth and development of the silkworm eggs are promoted. Before low-temperature storage, the silkworm eggs are subjected to anti-freezing protection by adopting a protection solution, and the sorbitol, the glycerol and the trehalose with ultralow freezing points are adopted, so that the freezing temperature of water in the silkworm eggs can be reduced, silkworm egg cells are protected from being frozen at low temperature, and the three substances are used as energy sources in various organisms, have no toxic or harmful effect on the cells, and are natural and environment-friendly; when the protective liquid is used, a layer of protective liquid is sprayed on the silkworm eggs, and after the silkworm eggs are dried in the air, a layer of protective liquid is sprayed on the silkworm eggs, so that a protective film with moderate thickness can be formed on the surfaces of the silkworm eggs, and the silkworm eggs can be prevented from freezing and have good air permeability. The silkworm eggs are preserved at the low temperature of-6 to-12 ℃, so that the silkworm eggs which grow first can be slowed down or stopped, and meanwhile, the eggs which grow slowly can continue to grow and mature at the low temperature, so as to promote uniform hatching; because most of the nutrients in the preferentially developed silkworm eggs are consumed, the development speed is slowed down or stopped under the action of the activating solution, the nutrients in the silkworm eggs which are developed later are accumulated more, and the biological enzymes and various nutrients in the activating solution can still keep the metabolic function, so that the development process of the preferentially developed silkworm eggs is followed up, the synchronization of the development cycles of all the silkworm eggs is realized, the uniformity of hatching is improved, and the hatching rate is stabilized to be more than 90% per day.
The activating solution of the invention firstly adopts phosphate buffer saline solution to soak the mulberry leaves, the orange peels, the bamboo leaves and the watermelon peels, so that the cells of the materials fully absorb water, and then reflux extraction is carried out, thereby completely extracting superoxide dismutase, catalase, amino acid, vitamin and trace elements in the cells quickly; then adding Turkey red oil, Tween 80 and ethyl acetate for ultrasonic treatment, so that ethyl acetate and Tween 80 form a proper proportion, and the extracted enzyme is subjected to mild activation or inhibition; finally, trehalose is added to protect the space structure of the biological enzyme from being damaged by low temperature, so that enzyme substances and various nutrient components are ensured to participate in the growth and development of silkworm eggs. In the silkworm eggs which grow faster, the nutrient substances are less, and the function of inhibiting the enzyme activity by the ethyl acetate is enhanced, so that the egg growth is stopped; in the silkworm eggs which grow slowly, more nutrient substances are contained, and the effect of the Tween 80 on improving the enzyme activity is enhanced, so that the eggs continue to grow and mature; meanwhile, Turkey red oil is a surfactant, plays a role in wetting and penetrating the surface of hydrophobic silkworm eggs, helps components in the activating solution to enter the silkworm eggs, enables the components to directly participate in physiological activities for regulating and controlling the growth and development of the silkworm eggs, and finally enables all the silkworm eggs in storage to develop synchronously.
The protective solution takes sorbitol, glycerol and trehalose as an antifreezing protective agent, protects the silkworm egg cells from being frozen at low temperature, and simultaneously protects the structure of protein in the cells from being stable and not damaged even at the low temperature of-6 to-12 ℃, adopts the bonding effect among cyclodextrin, egg white and chitosan to form a protective net with uniform thickness and proper density on the surface of the silkworm egg, and plays the effects of antifreezing and air permeability. The trehalose is wrapped in a hydrophobic structure inside the cyclodextrin, so that the trehalose can form stable and independent molecular individuals in the protective solution, and the protective effect of the trehalose on protein and cell walls in silkworm eggs is improved; by utilizing the hydrophobic characteristic that the hollow structures of the ethyl acetate and the cyclodextrin are similar, the ethyl acetate is used as an inclusion wetting agent, and the low temperature is kept to prevent the ethyl acetate from volatilizing rapidly, so that the trehalose is quickly wrapped by the cyclodextrin, and the inclusion rate is increased; then, trehalose included by the cyclodextrin is uniformly dispersed in the egg white by ultrasonic treatment, chitosan is added, so that hydroxyl on the outer surface of the cyclodextrin, amino residues in the egg white and hydroxyl and amino in the chitosan interact to form a series of covalent bonds under the ultrasonic treatment, and the covalent bonds are connected in series on the surface of the silkworm egg to form a protective net with uniform thickness and proper density, thereby achieving the effects of freezing prevention and ventilation.
3. The disinfection solution is prepared by extracting flavonoids such as artemisinin and decanoyl acetaldehyde and volatile oil bacteriostatic components from folium artemisiae argyi, sweet wormwood and houttuynia cordata and adding snake oil and eucalyptus oil to enhance the bacteriostatic effect, and can effectively protect silkworm eggs and prevent silkworm eggs from suffering from germs and insect pests. The snake oil can exert the advantage of good affinity between the snake oil and the egg shell cells, is beneficial to the fusion of disinfectant components and the egg shells, and has the effects of long-acting sterilization and bacteriostasis. The sterilization method is characterized in that an ethanol water solution is used as a solvent, and the sterilization method adopts a spraying or dipping method for sterilization, so that the sterilization function of ethanol and the strong volatility of the ethanol are utilized, the phenomenon that the silkworm eggs are too wet to obstruct the gas exchange between the inside and the outside of the silkworm eggs can be avoided, and the ethanol can also enhance the permeability of the silkworm egg shells and help the permeation of an activating solution.
4. After the silkworm eggs are laid, different protection methods are respectively adopted for the non-overyear eggs and the overyear eggs, so that the method is beneficial to fertilization, division and development of two different silkworm eggs. The non-overyear egg is protected at 22-26 ℃ for 45-52 hours, so that the embryo in the silkworm egg develops to the humanoid stage, various biological enzymes in the cell are completely synthesized, the vigorous vitality stage is reached, the low-temperature stress resistance of the silkworm egg can be increased, the temperature is reduced to 14-18 ℃ for protection for 9-17 hours, the activity of the enzymes is slowly reduced, the silkworm egg development is delayed, the silkworm egg is maintained at the strong low-temperature stress resistance stage, and the subsequent cold storage is convenient to maintain; the overyear egg is protected at 20-25 deg.c for 55-65 hr to make the overyear egg enter deep diapause after its development to yolk division stage, so that the silkworm egg is in strong low temperature stress resisting stage and growth arresting stage for subsequent preservation in cold storage.
5. The invention adopts low temperature and low humidity conditions during disinfection and activation, can maintain the lower development speed of the silkworm eggs, does not create conditions for the growth and development of the silkworm eggs, and avoids the problems that the silkworm eggs which are developed firstly develop too fast at higher temperature, the growth difference between the silkworm eggs which are developed slowly is enlarged, and the synchronization of the development and the one-day hatching rate of the silkworm eggs are influenced.
[ detailed description ] embodiments
The present invention will be further described with reference to the following examples.
Example 1
A preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear eggs and the non-overyear eggs, and then placing the silkworm eggs in a silkworm rearing room for development protection for 55 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 22 ℃ and the humidity of 70%, protecting for 46 hours, then adjusting the temperature of the silkworm rearing room to 14 ℃, and continuing to protect for 9 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing overyear eggs at 20 ℃ and the humidity at 76% for protection to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing eggs A and the developing eggs B by using a disinfectant, wherein the temperature of the disinfectant is 12 ℃ to obtain disinfected eggs;
the disinfectant comprises the following raw materials in parts by weight: 3 parts of folium artemisiae argyi, 3 parts of sweet wormwood herb, 2 parts of houttuynia cordata, 0.3 part of snake oil, 0.1 part of eucalyptus oil, 35 parts of acetone and 60 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 50%.
The preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 62 ℃ for 20 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 35 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
The using method of the disinfectant comprises the following steps: spraying the solution with volume of 3 ml/piece of silkworm egg.
c. Activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 6 ℃ and the humidity to 30%, then taking out sterilized eggs, soaking the eggs in an activating solution at 6 ℃ for 12 minutes, and then taking out the eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 80 parts of phosphate buffer solution, 5 parts of mulberry leaf, 6 parts of turkey red oil, 6 parts of ethyl acetate, 4 parts of orange peel, 2 parts of bamboo leaf, 1 part of watermelon peel, 802 parts of tween and 1 part of trehalose; the pH value of the phosphate buffer solution is 5.0;
the preparation method of the activating solution comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution for soaking for 50 minutes after crushing, then refluxing and extracting for 70 minutes at 50 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 8 minutes, setting the ultrasonic temperature at 25 ℃ and the ultrasonic power at 150W, then adding trehalose, and stirring uniformly to obtain an activation solution;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying activated eggs with a protective solution at 1 ℃, spraying the protective solution on the surfaces of the activated eggs for 1 time after the protective solution on the surfaces of the activated eggs is dried, drying the protective solution on the surfaces, and storing in a refrigeration house at-12 ℃; the protective solution comprises the following raw materials in parts by weight: 6 parts of sorbitol, 4 parts of glycerol, 3 parts of egg white, 4 parts of chitosan, 2 parts of cyclodextrin, 1 part of trehalose and 0.4 part of ethyl acetate;
the preparation method of the protective solution comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; taking trehalose, adding ethyl acetate at 4 ℃, stirring and wetting, then continuously keeping the temperature at 4 ℃, adding cyclodextrin, uniformly mixing, then stirring at 40 ℃ until no ethyl acetate smell exists, then adding egg white, carrying out ultrasonic treatment at 20 ℃ for 5 minutes, then adding chitosan, continuously carrying out ultrasonic treatment at 20 ℃ for 15 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Example 2
A preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear eggs and the non-overyear eggs, and then placing the silkworm eggs in a silkworm rearing room for development protection for 65 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 26 ℃ and the humidity of 74%, protecting for 52 hours, then adjusting the temperature of the silkworm rearing room to 18 ℃, and continuing to protect for 13 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing overyear eggs at 25 ℃ and the humidity at 82 percent for protection to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing eggs A and the developing eggs B by using a disinfectant, wherein the temperature of the disinfectant is 20 ℃, and obtaining disinfected eggs;
the disinfectant comprises the following raw materials in parts by weight: 15 parts of folium artemisiae argyi, 11 parts of sweet wormwood herb, 8 parts of houttuynia cordata, 0.7 part of snake oil, 0.5 part of eucalyptus oil, 65 parts of acetone and 100 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 60%.
The preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 72 ℃ for 40 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 45 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
The using method of the disinfectant comprises the following steps: spraying the solution with volume of 7 ml/piece of silkworm egg.
c. Activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to be 12 ℃ and the humidity to be 40%, then taking out the disinfected eggs, soaking the disinfected eggs in an activating solution at the temperature of 12 ℃ for 28 minutes, and then taking out the disinfected eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 140 parts of phosphate buffer solution, 15 parts of mulberry leaf, 12 parts of turkey red oil, 10 parts of ethyl acetate, 10 parts of orange peel, 7 parts of bamboo leaf, 5 parts of watermelon peel, 804 parts of tween and 3 parts of trehalose; the pH value of the phosphate buffer solution is 7.0;
the preparation method of the activating solution comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, crushing, adding phosphate buffer solution to soak for 70 minutes, then refluxing and extracting for 90 minutes at 60 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 16 minutes, setting the ultrasonic temperature to be 35 ℃ and the ultrasonic power to be 170W, adding trehalose, and stirring uniformly to obtain an activation solution;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying activated eggs with a protective solution at 5 ℃, spraying the protective solution on the surfaces of the activated eggs for 1 time after the protective solution on the surfaces of the activated eggs is dried, drying the protective solution on the surfaces, and storing in a refrigeration house at-6 ℃; the protective solution comprises the following raw materials in parts by weight: 12 parts of sorbitol, 12 parts of glycerol, 9 parts of egg white, 8 parts of chitosan, 8 parts of cyclodextrin, 7 parts of trehalose and 0.8 part of ethyl acetate;
the preparation method of the protective solution comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; adding ethyl acetate into trehalose, stirring and wetting at 10 ℃, continuously keeping the temperature at 10 ℃, adding cyclodextrin, uniformly mixing, stirring at 50 ℃ until no ethyl acetate smell exists, then adding egg white, carrying out ultrasonic treatment at 30 ℃ for 10 minutes, then adding chitosan, continuously carrying out ultrasonic treatment at 30 ℃ for 25 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Example 3
A preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear egg and the non-overyear egg, and placing the silkworm eggs in a silkworm rearing room for development protection for 68 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 23 ℃ and the humidity at 71%, protecting for 51 hours, then adjusting the temperature of the silkworm rearing room to 15 ℃, and continuing to protect for 17 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing overyear eggs at 21 ℃ and the humidity at 77% for protection to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing eggs A and the developing eggs B by using a disinfectant, wherein the temperature of the disinfectant is 14 ℃, and obtaining disinfected eggs;
the disinfectant comprises the following raw materials in parts by weight: 6 parts of folium artemisiae argyi, 5 parts of sweet wormwood herb, 3.5 parts of houttuynia cordata, 0.4 part of snake oil, 0.2 part of eucalyptus oil, 42 parts of acetone and 70 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 57%.
The preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 65 ℃ for 25 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 37 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
The using method of the disinfectant comprises the following steps: soaking silkworm egg and silkworm basket in disinfectant for 4 min.
c. Activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 7 ℃ and the humidity to 33%, then taking out the disinfected eggs, soaking the disinfected eggs in an activating solution at 8 ℃ for 16 minutes, and then taking out the disinfected eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 95 parts of phosphate buffer solution, 8 parts of mulberry leaf, 7.5 parts of turkey red oil, 7 parts of ethyl acetate, 5.5 parts of orange peel, 3.5 parts of bamboo leaf, 2 parts of watermelon peel, 802.5 parts of tween and 1.5 parts of trehalose; the pH value of the phosphate buffer solution is 5.5;
the preparation method of the activating solution comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution to soak for 55 minutes after crushing, then refluxing and extracting for 75 minutes at 52 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 10 minutes, setting the ultrasonic temperature at 27 ℃ and the ultrasonic power at 155W, adding trehalose, and stirring uniformly to obtain an activation solution;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying activated eggs with a protective solution at 2 ℃, spraying the protective solution on the surfaces of the activated eggs for 1 time after the protective solution on the surfaces of the activated eggs is dried, drying the protective solution on the surfaces, and storing in a refrigeration house at-8 ℃; the protective solution comprises the following raw materials in parts by weight: sorbitol 7.5 parts, glycerol 6 parts, egg white 4.5 parts, chitosan 5 parts, cyclodextrin 3.5 parts, trehalose 2.5 parts and ethyl acetate 0.5 part;
the preparation method of the protective solution comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; adding ethyl acetate into trehalose, stirring and wetting at 5 ℃, continuously keeping the temperature at 5 ℃, adding cyclodextrin, uniformly mixing, stirring at 43 ℃ until no ethyl acetate smell exists, then adding egg white, carrying out ultrasonic treatment at 22 ℃ for 6 minutes, then adding chitosan, continuously carrying out ultrasonic treatment at 22 ℃ for 18 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Example 4
A preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear eggs and the non-overyear eggs, and then placing the silkworm eggs in a silkworm rearing room for development protection for 62 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 25 ℃ and the humidity at 73%, protecting for 48 hours, then adjusting the temperature of the silkworm rearing room to 17 ℃, and continuing to protect for 14 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing overyear eggs at 24 ℃ and the humidity at 81 percent for protection to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing eggs A and the developing eggs B by using a disinfectant, wherein the temperature of the disinfectant is 18 ℃ to obtain disinfected eggs;
the disinfectant comprises the following raw materials in parts by weight: 12 parts of folium artemisiae argyi, 9 parts of sweet wormwood herb, 6.5 parts of houttuynia cordata, 0.6 part of snake oil, 0.4 part of eucalyptus oil, 58 parts of acetone and 90 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 57%.
The preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 70 ℃ for 35 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 42 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
The using method of the disinfectant comprises the following steps: soaking silkworm egg and silkworm basket in disinfectant for 10 min.
c. Activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 10 ℃ and the humidity to 37%, then taking out sterilized eggs, soaking the eggs in an activating solution at 10 ℃ for 24 minutes, and then taking out the eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 125 parts of phosphate buffer solution, 12 parts of mulberry leaf, 10.5 parts of turkey red oil, 9 parts of ethyl acetate, 8.5 parts of orange peel, 6 parts of bamboo leaf, 4 parts of watermelon peel, 803.5 parts of tween and 2.5 parts of trehalose; the pH value of the phosphate buffer solution is 6.5;
the preparation method of the activating solution comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution for soaking for 65 minutes after crushing, then refluxing and extracting for 85 minutes at 57 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 14 minutes, setting the ultrasonic temperature at 33 ℃ and the ultrasonic power at 165W, then adding trehalose, and stirring uniformly to obtain an activation solution;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying activated eggs with a protective solution at 4 ℃, spraying the protective solution on the surfaces of the activated eggs for 1 time after the protective solution on the surfaces of the activated eggs is dried, drying the protective solution on the surfaces, and storing in a refrigeration house at-10 ℃; the protective solution comprises the following raw materials in parts by weight: 10.5 parts of sorbitol, 10 parts of glycerol, 7.5 parts of egg white, 7 parts of chitosan, 6.5 parts of cyclodextrin, 5.5 parts of trehalose and 0.7 part of ethyl acetate;
the preparation method of the protective solution comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; adding ethyl acetate into trehalose, stirring and wetting at 8 ℃, continuously keeping the temperature at 8 ℃, adding cyclodextrin, uniformly mixing, stirring at 47 ℃ until no ethyl acetate smell exists, adding egg white, carrying out ultrasonic treatment at 28 ℃ for 9 minutes, adding chitosan, continuously carrying out ultrasonic treatment at 28 ℃ for 22 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Example 5
A preservation method of silkworm eggs comprises the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear eggs and the non-overyear eggs, and then placing the silkworm eggs in a silkworm rearing room for development protection for 60 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 24 ℃ and the humidity at 72%, protecting for 50 hours, then adjusting the temperature of the silkworm rearing room to 16 ℃, and continuing to protect for 10 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing overyear eggs at 22 ℃ and the humidity at 79 percent for protection to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing eggs A and the developing eggs B by using a disinfectant, wherein the temperature of the disinfectant is 16 ℃ to obtain disinfected eggs;
the disinfectant comprises the following raw materials in parts by weight: 9 parts of folium artemisiae argyi, 7 parts of sweet wormwood herb, 5 parts of houttuynia cordata, 0.5 part of snake oil, 0.3 part of eucalyptus oil, 50 parts of acetone and 80 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 55%.
The preparation method of the disinfectant comprises the following steps: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 67 ℃ for 30 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 40 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
The using method of the disinfectant comprises the following steps: soaking silkworm egg and silkworm basket in disinfectant for 7 min.
c. Activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 9 ℃ and the humidity to 35%, then taking out sterilized eggs, soaking the eggs in an activating solution at 9 ℃ for 20 minutes, and then taking out the eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 110 parts of phosphate buffer solution, 10 parts of mulberry leaves, 9 parts of turkey red oil, 8 parts of ethyl acetate, 7 parts of orange peels, 5 parts of bamboo leaves, 3 parts of watermelon peels, 803 parts of tween and 2 parts of trehalose; the pH value of the phosphate buffer solution is 6.0;
the preparation method of the activating solution comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution for soaking for 60 minutes after crushing, then refluxing and extracting for 80 minutes at 55 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 12 minutes, setting the ultrasonic temperature at 30 ℃ and the ultrasonic power at 160W, then adding trehalose, and stirring uniformly to obtain an activation solution;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying activated eggs with a protective solution at 3 ℃, spraying the protective solution on the surfaces of the activated eggs for 1 time after the protective solution on the surfaces of the activated eggs is dried, drying the protective solution on the surfaces, and storing in a refrigeration house at-9 ℃; the protective solution comprises the following raw materials in parts by weight: 9 parts of sorbitol, 8 parts of glycerol, 6 parts of egg white, 6 parts of chitosan, 5 parts of cyclodextrin, 4 parts of trehalose and 0.6 part of ethyl acetate;
the preparation method of the protective solution comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; adding ethyl acetate into trehalose, stirring and wetting at 7 ℃, continuously keeping the temperature at 7 ℃, adding cyclodextrin, uniformly mixing, stirring at 45 ℃ until no ethyl acetate smell exists, adding egg white, carrying out ultrasonic treatment at 25 ℃ for 7 minutes, adding chitosan, continuously carrying out ultrasonic treatment at 25 ℃ for 20 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
Example 6
The procedure is as in example 5 except that the disinfectant in step b is prepared using water instead of the aqueous ethanol solution.
Example 7
The procedure of example 5 was followed except that no snake oil was added to the disinfectant liquid prepared in step b.
Example 8
The procedure is as in example 5 except that no Turkish red oil is added to the activation solution in step c.
Example 9
The same procedure as in example 5 was repeated, except that Tween 80 was not added to the activating solution in step c.
Example 10
The same procedure as in example 5 was repeated, except that ethyl acetate was not added to the activation solution in step c.
Example 11
The same procedure as in example 5 was repeated except that the activation of the eggs in step c was not carried out.
Example 12
The procedure is as in example 5 except that egg white is not added to the protecting solution in step d.
Example 13
The same procedure as in example 5 was repeated except that egg white and chitosan were not added to the protecting solution in step d.
Example 14
The procedure is as in example 5 except that trehalose is not added to the protecting solution in step d.
Example 15
The procedure is as in example 5 except that no ethyl acetate is added to wet the protecting solution in step d.
Example 16
The procedure is as in example 5 except that the activated eggs are not sprayed with the protective solution in step d.
Example 17
The procedure is as in example 5 except that the temperature of the cold store in step d is-4 ℃.
Example 18
The procedure is as in example 5 except that the cold store temperature in step d is-14 ℃.
Example 19 comparison of effects
1.1. Preparation of experimental samples: the same batch of overyear eggs and overyear eggs are selected and the eggs are preserved according to the method of the embodiment 1-18 by counting 1 silkworm egg, so as to obtain the experimental samples 1-18.
2. The evaluation method comprises the following steps: in the experimental samples 1 to 18, the non-overyear eggs in the refrigerator were taken out at the 6 th month and the 12 th month of the storage, the overyear eggs in the refrigerator were taken out at the 6 th month, the 12 th month and the 18 th month of the storage, and after the conventional incubation and incubation were respectively performed on the taken out non-overyear eggs and overyear eggs, the daily hatching rate and the average body weight of the silkworm were counted, and the detailed results are shown in tables 1 and 2.
3. Evaluation results
TABLE 1 evaluation results of eggs of Vaccinium
TABLE 2 evaluation results of overyear eggs
4. Results of the experiment
As seen from the results of tables 1 and 2, the evaluation results of the experimental samples 1 to 5 are superior to those of the experimental samples 6 to 18.
The water is used as the solvent in the disinfectant in the experimental sample 6, so that the surface water of the silkworm egg shell is excessive, the air permeability is poor, and the one-day hatching rate and the weight of the newly-hatched silkworms are affected.
The disinfectant of the experimental sample 7 is not added with the snake oil, so that the soaking effect of the disinfectant is poor, the antibacterial effect is reduced, and the hatching rate of silkworm eggs per day and the weight of the newly-hatched silkworms are reduced.
In the activating solution of the experimental sample 8, turkey red oil is not added, so that the wettability and the permeability of the activating solution are reduced, the uniformity of the growth and development of silkworm eggs is influenced, and the daily hatchability of the silkworm eggs and the weight of the silkworm larvae are reduced.
The activation solution of the experimental sample 9 is not added with tween 80, and the activation solution of the experimental sample 10 is not added with ethyl acetate, which both affect the activity of the enzyme in the silkworm eggs, so that the activity of the enzyme in the slowly-developing silkworm eggs is not effectively activated, and the activity of the enzyme in the quickly-developing silkworm eggs is not effectively inhibited, so that the silkworm eggs are not developed uniformly, and the daily hatchability of the silkworm eggs and the weight of the silkworm larvae are reduced.
The silkworm eggs of the experimental sample 11 omit the activation step, so that the growth and development speed of the silkworm eggs is slowed, the maturation time is later, the development is slow, and the daily hatchability and the weight of the silkworm larvae are affected.
Egg white is not added to the protective solution of the experimental sample 12, egg white and chitosan are not added to the protective solution of the experimental sample 13, and trehalose is not added to the protective solution of the experimental sample 14, so that an effective protective film cannot be formed on the surface of the silkworm eggs, the low temperature resistance of the silkworm eggs is reduced, and the daily hatchability of the silkworm eggs and the weight of the silkworm larvae are reduced.
The protective solution of the experimental sample 15 is not added with ethyl acetate for wetting during preparation, so that the protective function of trehalose on proteins and cell walls is reduced, the compactness of a protective film on the surface of the silkworm egg is reduced, the low temperature resistance of the silkworm egg is reduced, and the hatching rate of the silkworm egg per day and the weight of the silkworm are reduced.
In the experimental sample 16, the protective solution is not sprayed, so that the surface of the silkworm egg lacks a low-temperature resistant protective film, and the silkworm egg has no low-temperature resistance, so that the daily hatching rate of the silkworm egg and the weight of the silkworm are low.
The preservation temperature of the experimental sample 17 is-4 ℃, the preservation temperature of the experimental sample 18 is-14 ℃, and the temperature is too high, so that the silkworm eggs grow prematurely in the preservation process and die due to loss of low-temperature resistance, and the hatching rate of the silkworm eggs per day and the weight of the silkworm larvae are reduced; when the temperature is too low, the silkworm eggs can not resist the extremely low temperature and die, so that the daily hatching rate of the silkworm eggs and the weight of the silkworm larvae are reduced.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (7)
1. A method for preserving silkworm eggs is characterized by comprising the following steps:
a. protection of silkworm eggs: collecting newly laid silkworm eggs, respectively placing the silkworm eggs on different silkworm trays according to the overyear egg and the non-overyear egg, and placing the silkworm eggs in a silkworm rearing room for development protection for 55-65 hours; the specific method for development protection comprises the following steps: setting the temperature of a silkworm rearing room for storing the non-overyear eggs at 22-26 ℃ and the humidity of 70-74%, protecting for 46-52 hours, then adjusting the temperature of the silkworm rearing room to 14-18 ℃, and continuing to protect for 9-17 hours to obtain developed eggs A; setting the temperature of a silkworm rearing room for storing the overyear eggs at 20-25 ℃ and the humidity of 76% -82%, and protecting to obtain developed eggs B;
b. disinfecting silkworm eggs: disinfecting the developing ovum A and the developing ovum B with a disinfectant at the temperature of 12-20 ℃ to obtain disinfected ovum;
c. activating silkworm eggs to obtain activated eggs: adjusting the temperature of a silkworm rearing room to 6-12 ℃ and the humidity to 30% -40%, then taking out the sterilized eggs, soaking the sterilized eggs in an activating solution at 6-12 ℃ for 12-28 minutes, and then taking out the sterilized eggs to obtain activated eggs; the activating solution comprises the following raw materials in parts by weight: 80-140 parts of phosphate buffer solution, 5-15 parts of mulberry leaf, 6-12 parts of Turkey red oil, 6-10 parts of ethyl acetate, 4-10 parts of orange peel, 2-7 parts of bamboo leaf, 1-5 parts of watermelon peel, 802-4 parts of tween and 1-3 parts of trehalose; the pH value of the phosphate buffer solution is 5.0-7.0;
d. and (3) after the activated eggs are subjected to anti-freezing protection, placing the activated eggs in a cold storage for storage: spraying the activated eggs with a protective solution at 1-5 ℃ against the activated eggs, spraying the protective solution for 1 time again after the protective solution on the surfaces of the activated eggs is dried in the air, drying the protective solution on the surfaces, and then storing the protective solution in a refrigeration house at-6 to-12 ℃; the protective solution comprises the following raw materials in parts by weight: 6-12 parts of sorbitol, 4-12 parts of glycerol, 3-9 parts of egg white, 4-8 parts of chitosan, 2-8 parts of cyclodextrin, 1-7 parts of trehalose and 0.4-0.8 part of ethyl acetate.
2. The method for preserving silkworm eggs according to claim 1, wherein the preparation method of the activating solution in the step c comprises the following steps: taking mulberry leaves, orange peels, bamboo leaves and watermelon peels according to the weight part ratio, cleaning and airing, adding phosphate buffer solution to soak for 50-70 minutes after crushing, then refluxing and extracting for 70-90 minutes at 50-60 ℃, filtering, collecting filtrate, then adding Turkey red oil, Tween 80 and ethyl acetate, carrying out ultrasonic treatment for 8-16 minutes, setting the ultrasonic temperature at 25-35 ℃ and the ultrasonic power at 150-170W, adding trehalose, and uniformly stirring to obtain the activation solution.
3. The method for preserving silkworm eggs according to claim 1, wherein the preparation method of the protective solution in the step d comprises the following steps: putting sorbitol into glycerol according to the weight part ratio, and uniformly stirring to obtain a mixed solution for later use; taking trehalose, adding ethyl acetate at 4-10 ℃, stirring and wetting, then continuously keeping the temperature at 4-10 ℃, adding cyclodextrin, uniformly mixing, then stirring at 40-50 ℃ until no ethyl acetate smell exists, then adding egg white, carrying out ultrasonic treatment at 20-30 ℃ for 5-10 minutes, then adding chitosan, continuously carrying out ultrasonic treatment at 20-30 ℃ for 15-25 minutes, finally adding the mixed solution, and uniformly stirring to obtain the protective solution.
4. The method for preserving silkworm eggs according to claim 1, wherein the disinfectant in the step b comprises the following raw materials in parts by weight: 3-15 parts of folium artemisiae argyi, 3-11 parts of sweet wormwood herb, 2-8 parts of houttuynia cordata, 0.3-0.7 part of snake oil, 0.1-0.5 part of eucalyptus oil, 35-65 parts of acetone and 60-100 parts of ethanol water solution; the volume percentage concentration of the ethanol water solution is 50-60%.
5. The method for preserving silkworm eggs according to claim 4, wherein the disinfectant is prepared by: taking folium artemisiae argyi, sweet wormwood herb and houttuynia cordata, adding acetone, carrying out reflux extraction at 62-72 ℃ for 20-40 minutes, then filtering, collecting filter residues, adding an ethanol water solution into the filter residues, carrying out microwave extraction for 35-45 minutes, filtering, and collecting filtrate to obtain an extracting solution; and then adding snake oil and eucalyptus oil into the extracting solution, and uniformly mixing to obtain the disinfectant.
6. The method for preserving silkworm eggs according to claim 1, 4 or 5, wherein the disinfectant is used by the method comprising the following steps: spraying the solution with volume of 3-7 ml/piece of silkworm egg.
7. The method for preserving silkworm eggs according to claim 1, 4 or 5, wherein the disinfectant is used by the method comprising the following steps: soaking silkworm egg and silkworm basket in disinfectant for 4-10 min.
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| CN114391518B (en) * | 2021-12-29 | 2022-11-25 | 广东省农业科学院蚕业与农产品加工研究所 | Silkworm egg hatching method based on warm water immersion |
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