CN109580962A - A kind of modified form triglyceride determination kit - Google Patents
A kind of modified form triglyceride determination kit Download PDFInfo
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- CN109580962A CN109580962A CN201910088765.8A CN201910088765A CN109580962A CN 109580962 A CN109580962 A CN 109580962A CN 201910088765 A CN201910088765 A CN 201910088765A CN 109580962 A CN109580962 A CN 109580962A
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- triglyceride determination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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Abstract
The invention discloses a kind of modified form triglyceride determination kits, it is related to medical detection field, it is linearly not high to solve TG in triglyceride determination reagent currently on the market, the problem of being influenced by dissociative glycerin, is characterized in that: being threadedly attached on body including body, bottle cap, positive stop strip, sealing film and reagent, bottle cap, positive stop strip is located between body and bottle cap, sealing film is fixedly connected on the bottle mouth position of positive stop strip, and reagent is located in body, and reagent is modified form triglyceride determination reagent；The present invention can solve the problem of anti-dissociative glycerin of clinical detection TG measurement reagent interferes, and provide the data closer to truth to patient, production technology is easier to control, and clinical application is simpler, and does not increase any cost.
Description
Technical field
The present invention relates to medical detection field more particularly to a kind of modified form triglyceride determination kits.
Background technique
Triglyceride kit is used to measure the content of triglycerides in patients serum or blood plasma, and lipoprotein lipase LP makes serum
Middle triglyceride hydrolysis is at glycerol and fatty acid, the glycerol glycerokinase of generation, suitable for the semi-automatic, complete of open channel
Automatic biochemistry analyzer, triglyceride determination kit in wide clinical application,
Lipase digestive is generally used in triglyceride reagent, but there are two disadvantages for the reagent: being easy to receive the shadow of dissociative glycerin
Ring, if the numerical value of patient's body dissociative glycerin more reagents measurement will be very high, cause clinical value to occur with it is practical not
The case where symbol, finally occurs failing to report, misrepresents deliberately phenomenon, therefore, we have proposed a kind of modified form triglyceride determination reagent, to
It solves the problem of the prior art TG is not linearly high in triglyceride determination reagent currently on the market to be influenced by dissociative glycerin.
Summary of the invention
The technical problem to be solved by the present invention is in view of the foregoing drawbacks, provide a kind of modified form triglyceride determination reagent
Box is influenced with solving the problem of in triglyceride determination reagent currently on the market that TG is not linearly high by dissociative glycerin.
To achieve the above object, the invention provides the following technical scheme:
A kind of modified form triglyceride determination kit, including body, bottle cap, positive stop strip, sealing film and reagent, bottle cap pass through spiral shell
Line is connected on body, and positive stop strip is located between body and bottle cap, and sealing film is fixedly connected on the bottle mouth position of positive stop strip, and reagent is set
In body, reagent is modified form triglyceride determination reagent.
As a further solution of the present invention, striker is equipped in the bottle cap, striker is fixedly connected on the bottle mouth position of bottle cap,
Liquid droping port is additionally provided on bottle cap.
As a further solution of the present invention, the agent formulations are sodium hydroxide, 4- hydroxyethyl piperazineethanesulfonic acid, acetic acid
Magnesium, EDETATE SODIUM, sodium azide, l-Alanine, trinosin, P-hydroxybenzoic acid sodium, sodium taurocholate, 4-AAP,
TTAB, Triton X-100 and oxide enzyme.
As a further solution of the present invention, the oxide enzyme includes BSA, cholesterol esterase, POD, phosphoglycerol oxygen
Change enzyme and glycerokinase.
As a further solution of the present invention, the component content of the reagent are as follows: sodium hydroxide 0.25g/L, 4- ethoxy
Piperazine ethanesulfonic acid 3.025g/L, magnesium acetate 1.6g/L, EDETATE SODIUM 0.75g/L, sodium azide 0.1g/L, l-Alanine 1g/L,
Trinosin 0.363g/L, P-hydroxybenzoic acid sodium 0.1g/L, sodium taurocholate 5g/L, 4-AAP0.025g/L,
TTAB0.5g/L, Triton X-100 2.5g/L.
As a further solution of the present invention, the oxide enzyme content are as follows: BSA0.5g/L, cholesterol esterase 10KU/L,
POD0.5KU/L, phosphoglycerol oxidase 2.5KU/L, glycerokinase 0.5KU/L.
As a further solution of the present invention, pH value is 7.2 ± 0.05 to the reagent at 20 degrees celsius.
In conclusion compared with the prior art, the invention has the following beneficial effects:
The present invention can solve the problem of anti-dissociative glycerin of clinical detection TG measurement reagent interferes, and provide patient closer to true feelings
The data of condition, production technology are easier to control, and clinical application is simpler, and do not increase any cost.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of modified form triglyceride determination kit.
Appended drawing reference: 1- body, 2- bottle cap, 3- positive stop strip, 4- striker, 5- seal film, 6- reagent.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
A kind of modified form triglyceride determination reagent, including sodium hydroxide, 4- hydroxyethyl piperazineethanesulfonic acid, magnesium acetate, EDTA bis-
Sodium, sodium azide, l-Alanine, trinosin, P-hydroxybenzoic acid sodium, sodium taurocholate, 4-AAP, TTAB, poly- second two
Alcohol octyl phenyl ether and oxide enzyme, the oxide enzyme include BSA, cholesterol esterase, POD, phosphoglycerol oxidase and sweet
Oily kinases；
Each component content in the modified form triglyceride determination reagent are as follows: sodium hydroxide 0.25g/L, 4- hydroxyethyl piperazine second sulphur
Sour 3.025g/L, magnesium acetate 1.6g/L, EDETATE SODIUM 0.75g/L, sodium azide 0.1g/L, l-Alanine 1g/L, Adenosine triphosphate
Glycosides disodium 0.363g/L, P-hydroxybenzoic acid sodium 0.1g/L, sodium taurocholate 5g/L, 4-AAP0.025g/L, TTAB0.5g/L, poly- second
Glycol octyl phenyl ether 2.5g/L；
Content of the oxide enzyme in modified form triglyceride determination reagent are as follows: BSA0.5g/L, cholesterol esterase 10KU/
L, POD0.5KU/L, phosphoglycerol oxidase 2.5KU/L, glycerokinase 0.5KU/L；
Preferably, described 20 degrees Celsius of lower pH values of modified form triglyceride determination reagent be 7.2, PH adjust reagent be PIPES with
Sodium hydroxide.
Embodiment 2
As shown in Figure 1, a kind of modified form triglyceride determination kit, including body 1, bottle cap 2, positive stop strip 3, sealing 5 and of film
Reagent 6, the body 1 are prior art reagent bottle, and bottle cap 2 is threadedly attached on body 1, and positive stop strip 3 is located at 1 He of body
Between bottle cap 2, sealing film 5 is fixedly connected on the bottle mouth position of positive stop strip 3, and reagent 6 is located in body 1, is sealed by sealing film 5；
It is equipped with striker 4 in the bottle cap 2, striker 4 is fixedly connected on the bottle mouth position of bottle cap 2, for poking sealing film 5, makes reagent 6
It is flowed out out of body 1, is additionally provided with liquid droping port on bottle cap 2, for oozing for reagent 6；
The reagent 6 is modified form triglyceride determination reagent described in embodiment 1.
In conclusion the working principle of the invention is:
In use, positive stop strip 3 of tearing, bottle cap 2 is tightened, striker 4 pokes sealing film 5, squeezes body 1, the reagent 6 in body 1
Just it is extruded.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of modified form triglyceride determination kit, including body (1), bottle cap (2), positive stop strip (3), sealing film (5) and
Reagent (6), bottle cap (2) are threadedly attached on body (1), and positive stop strip (3) is located between body (1) and bottle cap (2), sealing
Film (5) is fixedly connected on the bottle mouth position of positive stop strip (3), and reagent (6) is located in body (1), which is characterized in that reagent (6) is to change
Good figure triglyceride determination reagent.
2. modified form triglyceride determination kit according to claim 1, which is characterized in that set in the bottle cap (2)
Have striker (4), striker (4) is fixedly connected on the bottle mouth position of bottle cap (2), and bottle cap is additionally provided with liquid droping port on (2).
3. modified form triglyceride determination kit according to claim 2, which is characterized in that reagent (6) ingredient
For sodium hydroxide, 4- hydroxyethyl piperazineethanesulfonic acid, magnesium acetate, EDETATE SODIUM, sodium azide, l-Alanine, atriphos two
Sodium, P-hydroxybenzoic acid sodium, sodium taurocholate, 4-AAP, TTAB, Triton X-100 and oxide enzyme.
4. modified form triglyceride determination kit according to claim 3, which is characterized in that the oxide enzyme includes
BSA, cholesterol esterase, POD, phosphoglycerol oxidase and glycerokinase.
5. modified form triglyceride determination kit according to claim 4, which is characterized in that the reagent (6) at
Point content are as follows: sodium hydroxide 0.25g/L, 4- hydroxyethyl piperazineethanesulfonic acid 3.025g/L, magnesium acetate 1.6g/L, EDETATE SODIUM
0.75g/L, sodium azide 0.1g/L, l-Alanine 1g/L, trinosin 0.363g/L, P-hydroxybenzoic acid sodium
0.1g/L, sodium taurocholate 5g/L, 4-AAP0.025g/L, TTAB0.5g/L, Triton X-100 2.5g/L.
6. modified form triglyceride determination kit according to claim 4, which is characterized in that the oxide enzyme content
Are as follows: BSA0.5g/L, cholesterol esterase 10KU/L, POD0.5KU/L, phosphoglycerol oxidase 2.5KU/L, glycerokinase 0.5KU/
L。
7. -5 any modified form triglyceride determination kit according to claim 1, which is characterized in that the reagent
(6) pH value is 7.2 ± 0.05 at 20 degrees celsius.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910088765.8A CN109580962A (en) | 2019-01-30 | 2019-01-30 | A kind of modified form triglyceride determination kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910088765.8A CN109580962A (en) | 2019-01-30 | 2019-01-30 | A kind of modified form triglyceride determination kit |
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| Publication Number | Publication Date |
|---|---|
| CN109580962A true CN109580962A (en) | 2019-04-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910088765.8A Pending CN109580962A (en) | 2019-01-30 | 2019-01-30 | A kind of modified form triglyceride determination kit |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060058507A1 (en) * | 2002-05-10 | 2006-03-16 | Genset S.A. | Krib polynucleotides and polypeptides and uses thereof in the treatment of metabolic disorders |
| CN103913581A (en) * | 2013-11-20 | 2014-07-09 | 天津市宝坻区人民医院 | Cyclophorase determination method for triglyceride in serum |
| CN106399460A (en) * | 2016-09-29 | 2017-02-15 | 四川迈克生物科技股份有限公司 | Kit and method for determining triglyceride |
| CN206248666U (en) * | 2016-12-08 | 2017-06-13 | 深圳华中医学检验所有限公司 | For the kit of in-vitro diagnosis |
| CN108467882A (en) * | 2018-03-30 | 2018-08-31 | 潍坊市康华生物技术有限公司 | A kind of triglyceride detection kit |
| CN108949903A (en) * | 2017-05-17 | 2018-12-07 | 广州市伊川生物科技有限公司 | A kind of triglyceride determination kit and its measuring method |
-
2019
- 2019-01-30 CN CN201910088765.8A patent/CN109580962A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060058507A1 (en) * | 2002-05-10 | 2006-03-16 | Genset S.A. | Krib polynucleotides and polypeptides and uses thereof in the treatment of metabolic disorders |
| CN103913581A (en) * | 2013-11-20 | 2014-07-09 | 天津市宝坻区人民医院 | Cyclophorase determination method for triglyceride in serum |
| CN106399460A (en) * | 2016-09-29 | 2017-02-15 | 四川迈克生物科技股份有限公司 | Kit and method for determining triglyceride |
| CN206248666U (en) * | 2016-12-08 | 2017-06-13 | 深圳华中医学检验所有限公司 | For the kit of in-vitro diagnosis |
| CN108949903A (en) * | 2017-05-17 | 2018-12-07 | 广州市伊川生物科技有限公司 | A kind of triglyceride determination kit and its measuring method |
| CN108467882A (en) * | 2018-03-30 | 2018-08-31 | 潍坊市康华生物技术有限公司 | A kind of triglyceride detection kit |
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Application publication date: 20190405 |
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