CN204714784U - A kind of vaginitis combined test kit - Google Patents
A kind of vaginitis combined test kit Download PDFInfo
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- CN204714784U CN204714784U CN201520282589.9U CN201520282589U CN204714784U CN 204714784 U CN204714784 U CN 204714784U CN 201520282589 U CN201520282589 U CN 201520282589U CN 204714784 U CN204714784 U CN 204714784U
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Abstract
The utility model provides a kind of vaginitis combined test kit, and the upper plastic plate of its dry chemistry reaction unit has pH value hole, leukocyte esterase hole, peroxidation hydrogen holes, neuraminidase hole, N-Acetylhexosaminidase hole, GRD beta-glucuronidase hole; Lower plastic plate is fluted; Reactive group is in long strip shape groove, reactive group has peroxidation hydrogen holes reaction substrate cured layer, leukocyte esterase hole reaction substrate cured layer, neuraminidase hole reaction substrate cured layer, pH value hole reaction substrate cured layer, N-Acetylhexosaminidase hole reaction substrate cured layer and GRD beta-glucuronidase hole reaction substrate cured layer.The utility model manufactures and uses and all can facilitate, and can assess the pH value of person under inspection's reproductive tract, bacterial vaginosis pathogenic bacterium situation, degree of cleaning, micro-ecological environment, monilial vaginitis, trichomonal vaginitis, aerobism vaginitis simultaneously; It is simple to operate, and detection time is short, without the need to special instrument, is applicable to the use of routine clinical particularly hospital outpatient.
Description
Technical field
The utility model relates to a kind of vaginitis combined test kit.
Background technology
Bacterial vaginosis (Bacterial Vaginosis is called for short BV) is one of gynaecology's three kinds of modal diseases.The pathogenic agent of BV can produce virulence factor and cause epithelial cell damage, liquid is oozed out and vagina squamous cell comes off, and produces the secretory product of typical BV.The bacterial adhesion such as Gartner bacterium form clues cell on the vaginal epithelial cell come off.When corresponding change occurs the biochemical component of vaginal secretions, pH value then raises.The lactobacillus that lactobacillus especially produces hydrogen peroxide is very crucial to the ecological environment maintaining normal vagina, but alkaline environment is unfavorable for sticking of Bacterium lacticum and grows, and is beneficial to the growth of the anerobes such as Gardnerella, thus causes bacterial vaginosis.Gravid woman's sickness rate is high, points out relevant to estrogen level.Its pathological characters NIP pathology and leukocyte infiltration.Enzyme and organic acid can destroy the defense mechanism of host, as dissolved Ovulation prediction, can promote that microorganism enters reproductive tract, causing upper urogenital tract infection.
The modal symptom of monilial infection is that leukorrhea is many, vulva and the scorching hot itch of vagina, exogen misnicturition, vulva ground pattern erythema (mycotic or candidal vulvovaginitis).Typical leukorrhea is curdy or is sheet bulk, and vaginal mucosa height is red and swollen, and visible white white mouth sample patch adheres to, and easily peels off, and its lower rotten to the corn substrate for impaired mucous membrane, or formation fester, severe patient can leave over ecchymosis.But leukorrhea not all there is above-mentioned characteristic feature, from water sample until curdled milk sample leukorrhea all can occur, if any be some thin serous exudate infused clearly completely, wherein often containing white plates.The Gestation period, the pruritis of colpitis mycotica was particularly serious, and even feel restless, misery is abnormal, also can have the symptoms such as frequent micturition, odynuria and sexual anhedonia.In addition, though still have the women of about 10% and 30% pregnant woman to be mould carrier, without any clinical manifestation.
Trichomonal vaginitis is caused by trichomonas, and the trichomonas of parasitic human body has Trichomonas vaginalis, Trichomonas hominis and buccalis, parasitizes urogenital system, enteron aisle and oral cavity respectively, and relevant with tetter is Trichomonas vaginalis, causes trichomonal vaginitis.Be a kind of parasitic disease propagated mainly through sexual intercourse, there is infectivity.Number case is asymptomatic, and women has uncomfortable sensation may continue 1 week or some months, then can be clearly better because of menstruation or pregnancy, vaginal mucosa inflammation, in bright red, above covers patch shape pseudomembrane, normal companion's foam sample secretory product, conscious itch in various degree, minority has burning sensation.Leucorrhea increasing turns yellow green.Occasionally can there is frequent micturition, urgent urination, odynuria, blood urine, or stomachache, diarrhoea, mucus just, or teeth groove pyorrhea, carious tooth.Often cause urethritis, urocystitis, bartholinitis can be caused.
Along with the development of laboratory medicine, detect the New Set of vaginal secretions: neuraminidase, hydrogen peroxide, leukocyte esterase, pH value, N-Acetylhexosaminidase, GRD beta-glucuronidase have started the even alternative old index of complementation and be applied to clinical.Neuraminidase is the specific enzymes that Prey irrigates the BV pathogenic bacterium secretions such as bacterium, bacterioide, bacterium protein of Gardnerella vaginalis, and the positive shows there is bacterial vaginosis; The concentration of hydrogen peroxide is the important indicator of reflection microecology in vaginas balance, produces H
2o
2lactobacillus be maintain microecology in vaginas balance dominant bacteria; Leukocyte esterase is the esterase of polymorphonuclear leukocyte release, and under state of health, content is low.When body is inflamed and reacts, due to the chemotaxis of polymorphonuclear leukocyte, assemble and discharge leukocyte esterase in a large number at inflammatory lesions, the content therefore detecting vagina LE can reflect the degree of colpitis; PH value is the important indicator of reflection microecology in vaginas balance.Research shows that Candida albicans and trichomonad have N-Acetylhexosaminidase specific enzymes, be not more than 4.6, and trichomonad leukorrhea pH is not less than 4.8, by the two being distinguished in conjunction with pH value by large quantity research candidiasis leukorrhea pH.Therefore trichomonad can be detected by detection N-Acetylhexosaminidase and pH value.GRD beta-glucuronidase is a kind of specific enzymes that intestinal bacteria produce.Research in recent years finds, in enterobacteriaceae, the intestinal bacteria of 94% ~ 97% can produce GRD beta-glucuronidase, therefore the existence detecting this enzyme can the qualitative or colibacillary existence of detection by quantitative.
Summary of the invention
Technical problem to be solved in the utility model is to provide a kind of vaginitis combined test kit, its manufacture and use all convenient.For this reason, the utility model is by the following technical solutions:
A kind of vaginitis combined test kit, comprise dry chemistry reaction unit, it is characterized in that described dry chemistry reaction unit is made up of upper and lower two pieces of plastic plates and reactive group, wherein go up plastic plate and have six through holes, be respectively pH value hole, leukocyte esterase hole, peroxidation hydrogen holes, neuraminidase hole, N-Acetylhexosaminidase hole, GRD beta-glucuronidase hole; Lower plastic plate has a correspondence to be in groove below plastic plate six through holes; Described reactive group is in described groove, described reactive group is made up of one piece of carrier layer and six deblocking reaction substrate cured layers, and described six pieces of anti-thing cured layers are respectively peroxidation hydrogen holes reaction substrate cured layer, leukocyte esterase hole reaction substrate cured layer, neuraminidase hole reaction substrate cured layer, pH value hole reaction substrate cured layer, N-Acetylhexosaminidase hole reaction substrate cured layer and GRD beta-glucuronidase hole reaction substrate cured layer; Described six deblocking reaction substrate cured layers to be fixed on carrier layer and to be in correspondingly respectively below one of them through hole of described six through holes.
On the basis adopting technique scheme, the utility model also can adopt following further technical scheme:
Described groove and carrier layer are long strip shape, and the corresponding position, position of described six through holes is above them.
Described six deblocking reaction substrate cured layers are by glass fiber filter paper, and cellulosic filter paper, chromatographic paper, polyester film or nylon membrane bag are dried cutting by substrate solution and formed.
Described carrier layer is by simultaneously forming with the one in sticking PET, PVC, PE or PS sheet material cutting.
Have spacing between adjacent reaction substrate cured layer, spacing is: 5mm-15mm.
Upper plastic plate is fixedly connected with the form of draw-in groove with lower plastic plate.
Owing to adopting the technical solution of the utility model, vaginitis combined test kit of the present invention is very convenient during fabrication, yield is high, avoid adding one by one cumbersome process and latent fault risk that reactive group causes, can Simultaneously test concentration of hydrogen peroxide, pH value, leukocyte esterase concentration, neuraminidase concentration, N-Acetylhexosaminidase, β-glucuronic acid enzyme, centralized displaying measurement result, the micro-ecological environment of assessment person under inspection reproductive tract, pH value, degree of cleaning, bacterial vaginosis pathogenic bacterium situation, monilial infection, trichomonacide and aerobic-type pathogenic infection, simple to operate, accuracy is high, the operating time is short, without the need to special instrument, is applicable to the use of routine clinical particularly hospital outpatient.
Accompanying drawing explanation
Fig. 1 is the front view of plastic plate on the utility model combined test kit for bacterial vagina disease dry reaction device.
Fig. 2 is the front view of plastic plate under the utility model combined test kit for bacterial vagina disease dry reaction device.
Fig. 3 is the front view of the utility model combined test kit for bacterial vagina disease dry reaction device reaction base.
Fig. 4 is the sectional view of the utility model combined test kit for bacterial vagina disease dry reaction device.
Embodiment
Illustrate the utility model further by the following examples, it should be understood that these example examples are only for the object of illustration, never limit scope of the present utility model.
With reference to accompanying drawing.A kind of vaginitis combined test kit of the utility model, comprise dry chemistry reaction unit, described dry chemistry reaction unit is made up of upper and lower two pieces of plastic plates 1,2 and long strip shape reactive group 3, wherein go up plastic plate 1 and have six through holes, be respectively pH value hole 11, leukocyte esterase hole 12, peroxidation hydrogen holes 13, neuraminidase hole 14, N-Acetylhexosaminidase hole 15, GRD beta-glucuronidase hole 16; A correspondence is had to be in long strip shape groove 20 below plastic plate six through holes in the middle of lower plastic plate 2; Long strip shape reactive group 3 is in long strip shape groove 20, long strip shape reactive group 3 is made up of the carrier layer 30 of a block length bar shaped and six deblocking reaction substrate cured layers, and described six pieces of anti-thing cured layers are respectively peroxidation hydrogen holes reaction substrate cured layer 33, leukocyte esterase hole reaction substrate cured layer 32, neuraminidase hole reaction substrate cured layer 34, pH value hole reaction substrate cured layer 31, N-Acetylhexosaminidase hole reaction substrate cured layer 35 and GRD beta-glucuronidase hole reaction substrate cured layer 36; Described six deblocking reaction substrate cured layers to be fixed on carrier layer and to be in correspondingly respectively below one of them through hole of described six through holes.
The reaction substrate cured layer 33 of peroxidation hydrogen holes is coated with 4-amino antipyrine, DBHS or tetramethyl benzidine, sucrose, horseradish peroxidase and phosphate buffered saline buffer.Each material concentration is as follows:
Amino antipyrine: the 250-800mg/m of 4-
2; DBHS: 2-11.5g/m
2or tetramethyl benzidine: 5-12.9g/m
2; Sucrose: 25-40mg/m
2; Horseradish peroxidase: 5000000-725000U/m
2; Phosphate buffered saline buffer: 40mol/m
2.
Described leukocyte esterase hole reaction substrate cured layer 32 is coated with the chloro-3-indolyl acetic acid ethyl ester of the bromo-4-of 5-, sucrose, dehydrated alcohol and phosphate buffered saline buffer.Each material concentration is as follows:
The chloro-3-indolyl acetic acid ethyl ester of the bromo-4-of 5-: 200-800mg/m
2; Sucrose: 25-40mg/m
2; Dehydrated alcohol 25-40ml/m
2; Phosphate buffered saline buffer: 40mol/m
2.
Described neuraminidase hole reaction substrate cured layer 34 is coated with the chloro-3-indoles of the bromo-4-of 5--α-D-N-n acetylneuraminic acid n sodium salt, sucrose, acetic acid-sodium acetate buffer solution.Each material concentration is as follows:
The chloro-3-indoles of the bromo-4-of 5--α-D-N-n acetylneuraminic acid n sodium salt: 100-325mg/m
2; Sucrose: 40-76mg/m
2; Acetic acid-sodium acetate buffer solution: 50mol/m
2;
Described pH value hole reaction substrate cured layer 31 is commercially available accurate pH test paper.
Described N-Acetylhexosaminidase hole reaction substrate cured layer 35 is coated with the chloro-3-indoles of the bromo-4-of 5--β-D-N-N-Acetylhexosaminidase, sucrose, dehydrated alcohol, phosphate buffered saline buffer.Each material concentration is as follows:
The chloro-3-indoles of the bromo-4-of 5--β-D-N-N-Acetylhexosaminidase: 400-650mg/m
2; Sucrose: 50-85 mg/m
2; Dehydrated alcohol: 30-55ml/m
2; Phosphate buffered saline buffer: 40mol/m
2.
Described GRD beta-glucuronidase hole reaction substrate cured layer 36 is coated with the chloro-3-indoles-beta-glucuronidase of the bromo-4-of 5-, sucrose, dimethyl sulfoxide (DMSO), phosphate buffered saline buffer.Each material concentration is as follows:
The chloro-3-indoles-beta-glucuronidase of the bromo-4-of 5-: 380-580 mg/m
2; Sucrose: 40-76mg/m
2; Dimethyl sulfoxide (DMSO): 25-45ml/m
2; Phosphate buffered saline buffer: 40mol/m
2.
Described carrier layer 30 is by simultaneously forming with the one in sticking PET, PVC, PE or PS sheet material cutting.Size is as follows:
Long: 40mm-100mm, preferably: 50mm-80mm;
Wide: 1mm-10mm, preferably: 4mm-8mm;
Thickness: 0.1mm-1mm, preferably: 0.2mm-0.5mm.
On it, six deblocking reaction substrate cured layer spacing of sticky note are: 5mm-15mm, preferred 8mm-12mm.
Upper plastic plate and lower plastic plate adopt the mode of draw-in groove to be fixedly connected with.
Also have diluent and nitrite ion in test kit, described diluent is the phosphate buffer soln of pH=6.00 ± 0.10 containing 0.9% sodium-chlor, and described nitrite ion is the sodium hydroxide solution containing 0.5%-1.5% NBT.
Test kit of the present utility model is used to carry out the use step of vaginitis simultaneous determination as follows:
(1) test kit balance is taken out to room temperature;
(2) tear aluminium foil packing, take out the utility model reaction unit and take aluminium foil strip of paper used for sealing gently off;
(3) the reaction substrate cured layer of sampling cotton swab first below pH value hole (being also pH value hole reaction substrate cured layer 31) point is printed, observations immediately, then add 400ul diluent in test tube, repeatedly extrude tube wall, object is fully disengaged;
(4) the reaction substrate cured layer below other five through holes of reaction unit respectively drips the sample that (35ul) processed;
(5) cured layer below neuraminidase hole drips a nitrite ion, then reaction unit is positioned over the 45 DEG C of dry bath of constant temperature upper 15 minutes, result of determination.
The explanation of assay
1) peroxidation hydrogen holes: the reaction substrate cured layer display redness below it or light red are negative, and instruction possibility vaginal microbial flora is normal, does not change color as the positive, indicate flora to lack of proper care
2) neuraminidase hole: the reaction substrate cured layer below it does not change color as feminine gender; Display look purplish grey, brown or black are positive, and express possibility bacterial infection vaginosis.
3) leukocyte esterase hole: the reaction substrate cured layer nondiscoloration below it is negative; Display blue-greenish colour, blueness are positive, show that patient's intravaginal has inflammatory reaction in various degree.
4) pH value hole: the reaction substrate cured layer display yellow below it or yellow-green colour are feminine gender; Show sample pH value≤4.5, show dark green, blue-greenish colour or blueness, be the positive, show sample pH value >4.5.
5) N-Acetylhexosaminidase hole: the reaction substrate cured layer display below it is colourless, is feminine gender; Aobvious yellow-green colour, green, if pH≤4.6, prompt for monilial vaginitis, if pH >=4.8, prompt for trichomonal vaginitis.
6) GRD beta-glucuronidase: the reaction substrate cured layer display below it is colourless is negative; Display blueness is positive, represents aerobism vaginitis.
The foregoing is only preferred embodiment of the present utility model, is only illustrative for the utility model, and nonrestrictive; Those of ordinary skill in the art understand, and can carry out many changes, amendment, but all fall into protection domain of the present utility model in the spirit and scope that the utility model claim limits to it.
Claims (6)
1. a vaginitis combined test kit, comprise dry chemistry reaction unit, it is characterized in that described dry chemistry reaction unit is made up of upper and lower two pieces of plastic plates and reactive group, wherein go up plastic plate and have six through holes, be respectively pH value hole, leukocyte esterase hole, peroxidation hydrogen holes, neuraminidase hole, N-Acetylhexosaminidase hole, GRD beta-glucuronidase hole; Lower plastic plate has a correspondence to be in groove below plastic plate six through holes; Described reactive group is in described groove, described reactive group is made up of one piece of carrier layer and six deblocking reaction substrate cured layers, and described six pieces of anti-thing cured layers are respectively peroxidation hydrogen holes reaction substrate cured layer, leukocyte esterase hole reaction substrate cured layer, neuraminidase hole reaction substrate cured layer, pH value hole reaction substrate cured layer, N-Acetylhexosaminidase hole reaction substrate cured layer and GRD beta-glucuronidase hole reaction substrate cured layer; Described six deblocking reaction substrate cured layers to be fixed on carrier layer and to be in correspondingly respectively below one of them through hole of described six through holes.
2. a kind of vaginitis combined test kit as claimed in claim 1, is characterized in that described groove and carrier layer are long strip shape, and the corresponding position, position of described six through holes is above them.
3. a kind of vaginitis combined test kit as claimed in claim 1, is characterized in that described six deblocking reaction substrate cured layers are by glass fiber filter paper, cellulosic filter paper, chromatographic paper, and polyester film or nylon membrane bag are dried cutting by substrate solution and formed.
4. a kind of vaginitis combined test kit as claimed in claim 1, is characterized in that described carrier layer is by simultaneously forming with the one in sticking PET, PVC, PE or PS sheet material cutting.
5. a kind of vaginitis combined test kit as claimed in claim 1, it is characterized in that there is spacing between adjacent reaction substrate cured layer, spacing is: 5mm-15mm.
6. a kind of vaginitis combined test kit as claimed in claim 1, is characterized in that plastic plate is fixedly connected with the form of draw-in groove with lower plastic plate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520282589.9U CN204714784U (en) | 2015-05-04 | 2015-05-04 | A kind of vaginitis combined test kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520282589.9U CN204714784U (en) | 2015-05-04 | 2015-05-04 | A kind of vaginitis combined test kit |
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| CN204714784U true CN204714784U (en) | 2015-10-21 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970218A (en) * | 2017-05-27 | 2017-07-21 | 深圳市瀚德标检生物工程有限公司 | A kind of vaginitis self-test device |
| CN107389940A (en) * | 2017-08-23 | 2017-11-24 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit and preparation method thereof |
| CN111690713A (en) * | 2020-06-01 | 2020-09-22 | 青岛华晶生物技术有限公司 | Eight joint inspection kit of vaginitis |
-
2015
- 2015-05-04 CN CN201520282589.9U patent/CN204714784U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970218A (en) * | 2017-05-27 | 2017-07-21 | 深圳市瀚德标检生物工程有限公司 | A kind of vaginitis self-test device |
| CN106970218B (en) * | 2017-05-27 | 2020-01-03 | 深圳市瀚德标检生物工程有限公司 | Vaginitis self-detection device |
| CN107389940A (en) * | 2017-08-23 | 2017-11-24 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit and preparation method thereof |
| CN111690713A (en) * | 2020-06-01 | 2020-09-22 | 青岛华晶生物技术有限公司 | Eight joint inspection kit of vaginitis |
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