CN109566324A

CN109566324A - A kind of seed selection cultivating technique of gold lotus

Info

Publication number: CN109566324A
Application number: CN201811406015.2A
Authority: CN
Inventors: 陈剑虹
Original assignee: Quanzhou Golden Grass Biotechnology Co Ltd
Current assignee: Quanzhou Golden Grass Biotechnology Co Ltd
Priority date: 2018-11-23
Filing date: 2018-11-23
Publication date: 2019-04-05
Anticipated expiration: 2038-11-23
Also published as: CN109566324B

Abstract

The present invention proposes a kind of seed selection cultivating technique of gold lotus, breaks through the cultivating technique form of traditional roxburgh anoectochilus terminal bud, obtains the embryo of the fruit of picking, and embryo is placed in culture in culture medium and obtains rooted plantlet；The selection of first stage color variety is carried out to multiple rooted plantlets, selects the plurality of rooted plantlet with yellow blade as the first rank target seedling；The selection of second stage color variety is carried out after carrying out culture a period of time to multiple first rank target seedlings, selects at least one most yellow first rank target seedling of wherein blade as second-order target seedling；Then second-order target seedling cultivate up to yielding positive results, and picked to fruit maturation, obtain the embryo of the fruit of picking, and then go out the gold lotus of multiple golden yellow blades by multiple Seed embryo cultures；Or cell culture is carried out to second-order target seedling, obtain the gold lotus of multiple golden yellow blades.Compared with prior art, the present invention can carry out efficient and stable gold lotus breed of variety.

Description

A kind of seed selection cultivating technique of gold lotus

Technical field

The present invention relates to plant cultivating method fields, and in particular to a kind of seed selection cultivating technique of gold lotus.

Background technique

The breeding method of roxburgh anoectochilus terminal bud has been known, and such as Chinese invention patent, it is wild that CN103782905B discloses a kind of roxburgh anoectochilus terminal bud Outer seed selection and breeding method, specific steps include: the selection of 1) explant

Between annual September-November, roxburgh anoectochilus terminal bud opens the time spent, we select it is wild there is merit to have bloomed, Healthy and strong roxburgh anoectochilus terminal bud after carrying out artificial pollination, forms fruit pod；Full, uncracked fruit pod is picked as explant；2) explant Sterile working the fruit pod of picking is cleaned up after, sterile working is carried out, to disappearing outside fruit pod in aseptic operating platform After poison, fruit pod is opened, the seed of the inside is inoculated on roxburgh anoectochilus terminal bud culture medium；3) incubation step places the explant after inoculation At 24-26 DEG C of constant temperature, dark culture 10-15 days, after seed starts growth, illumination is carried out, into routine culture；It is conventional The growing way of seed is observed in incubation in time, then is judged, the industrialized propagation stage is finally entered；The roxburgh anoectochilus terminal bud training Support based formulas are as follows: spend precious No.1 2500-3000mg/L, peptone 1500-2000mg/L, niacin 1-2mg/L, vitaminB10 .5- 1mg/L, vitamin B6 1-2mg/L, amion acetic acid 50-60mg/L, inositol 50-60mg/L, coconut milk 50000-60000mg/L, Apple 50000-60000mg/L, white sugar 20000-30000mg/L, agar 10000-20000mg/L, pH:5-6 add water to be configured to 1000mL solution.The disinfection specific steps are as follows: fruit pod is put into the bleaching liquid that mass fraction is 12-15% and sufficiently shakes 3- 5 minutes, then after standing 5-7 minutes, then shake 2-3 minutes, whole process 10-15 minutes, i.e., appearance fruit pod is sterilized and is completed.Institute State the fruit pod that fruit pod is 45-55 days after pollination.

Survival rate can be improved in the roxburgh anoectochilus terminal bud seedling that the invention is cultivated, but it is similar with other breeding methods in the market, all Gold lotus (roxburgh anoectochilus terminal bud of golden yellow blade) can not be cultivated, and in fact, gold lotus in medical value and in the market more It is easy to get approval.

In consideration of it, inventor furthers investigate the above problem, there is this case generation then.

Summary of the invention

The purpose of the present invention is to provide the choosings that one kind can carry out the gold lotus of efficient and stable gold lotus breed of variety Kind cultivating technique.

In order to achieve the above object, the present invention uses such technical solution:

A kind of seed selection cultivating technique of gold lotus, includes the following steps:

(1) the whole strain balled transplanting of field roxburgh anoectochilus terminal bud plant by multiple with yellow blade is into artificial greenhouse, in gold thread The lotus plant florescence carries out artificial pollination, is picked to fruit maturation；

(2) embryo is placed in culture in culture medium and obtains rooted plantlet by the embryo for obtaining the fruit of picking；

(3) selection of first stage color variety is carried out to multiple rooted plantlets, selected plurality of with yellow blade Rooted plantlet is as the first rank target seedling；

The selection of second stage color variety is carried out after carrying out culture a period of time to multiple first rank target seedlings, is selected wherein At least one most yellow first rank target seedling of blade is as second-order target seedling；

(4) then second-order target seedling cultivate until yielding positive results, and picked to fruit maturation, acquisition is adopted The embryo for the fruit plucked, and then go out by multiple Seed embryo cultures the gold lotus of multiple golden yellow blades；

Or cell culture is carried out to second-order target seedling, obtain the gold lotus of multiple golden yellow blades.

Between the step (3) and (4), carries out color variations and reject operation, to the second-order target seedling culture one Fix time, to yellow blade gradually tail off or blade in the gradually smaller second-order target seedling of yellow area area accounting pick Remove, only leave yellow blade do not tail off or blade in the second-order target seedling that does not become smaller of yellow area area accounting.

In the step (2), operation is followed the steps below,

Embryo sowing is cultivated in germination medium, obtains protocorm by S1, the embryo for obtaining the fruit picked；

S2, protocorm is transferred in differential medium and is cultivated, obtain sprout；

S3, sprout is transferred in the strong culture medium of increasing and is cultivated, obtain strong sprout；

S4, strong sprout is transferred in root media and is cultivated, obtain rooted plantlet；

S5, rooted plantlet is taken out one by one and is transplanted.

In the step S1, first dark at a temperature of humidity 60-65%, 24-25 DEG C of temperature to train 12-14 days, then in light Under the conditions of intensity 1000-1500Lux, culture 23-25 days is carried out with daily 12 hours light application times；

In the step S2, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 1500-2500Lux, Culture 23-25 days is carried out with daily 12 hours light application times；

In the step S3, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 2000-3000Lux, Culture 30 days is carried out with daily 12 hours light application times.

In the step (3), first stage color variety is carried out using seed selection breeding apparatus and is chosen and second stage face Chromaticity kind is chosen；

The seed selection breeding apparatus includes the first rank device for carrying out the selection of first stage color variety, carries out second stage The second-order device that color variety is chosen receives the terminal installation of second-order target seedling, and fills to the first rank device, second-order Set the controller controlled with terminal installation；

The first rank device includes multiple parallel the first rank culture unit rows；

The first rank culture unit row includes the first rank conveyer belt and multiple the first ranks along the arrangement of the first rank conveyer belt Cultivate unit group；Each first rank conveyer belt is parallel；

The first rank culture unit group includes the first conveying rotating disc for carrying multiple culture buckets, incudes the first conveying rotating disc Turned position and the first position sensor that position signal is transferred to the controller, first of the first conveying rotating disc of support Dagger, the first device of rotation driving that the first conveying rotating disc of driving is rotated using the first support column as shaft, by the first conveying Culture bucket on turntable is pushed to the first pushing meanss on the first rank conveyer belt, and detection rooted plantlet leaf color The first color detection means；

The edge of first conveying rotating disc is equipped with sliding between the first conveying rotating disc and the first rank conveyer belt for culture bucket The first dynamic elasticity transition side, first conveying rotating disc are formed with the first rotation hole being inserted on first support column；Institute Stating the first device of rotation driving includes the first driven gear being placed on first support column, driving the first driven gear rotation The first driving gear, driving the first driving gear rotation first motor；The upper end of first driven gear and described the The lower surface of one conveying rotating disc links together, and first driving gear matches with first driven gear and is engaged on one It rises；First support column, which is equipped with, to be resisted against the first of first driven gear lower end and supports block, first driven gear And first support between block be equipped with first bearing；First pushing meanss include being connected to first upper end of support column and court To the first fluid cylinder pressure of corresponding first rank conveyer belt, and set on the first push plate of first fluid cylinder pressure output end；Described first For the output end of fluid-pressure cylinder towards culture bucket, the first fluid cylinder pressure and the first rank conveyer belt are perpendicular；

The first rank conveyer belt includes first that first conveyer belt main body and driving first conveyer belt main body are gone up and down Lifting drive；The first conveyer belt main body includes the first driving pulley, the first driven pulley, is connected to the first main belt First feeder motor of first conveyer belt and driving the first driving pulley rotation between wheel and the first driven pulley；It is described First lifting drive includes the first lifting support column of support first driving pulley and the first driven pulley, and carrying is each First lifting support plate of the first lifting support column, and the first lifting fluid pressure that driving the first lifting support plate is gone up and down Cylinder；

First color detection means include being transferred to the controller above culture bucket and by image data First color image pickup apparatus supports the first gooseneck boom of the first color image pickup apparatus, supports the first column of the first gooseneck boom；Described One color image pickup apparatus includes recess the first vault cover directed downwardly and multiple the first colored camera shootings being distributed in the first vault cover recess Head；

The second-order device includes and each perpendicular second-order conveyer belt of the first rank conveyer belt and multiple edges The arrangement of second-order conveyer belt and second-order culture unit group corresponding with the end of each first rank conveyer belt；

The second-order culture unit group includes the second conveying rotating disc for carrying multiple culture buckets, incudes the second conveying rotating disc Turned position and the second position sensor that position signal is transferred to the controller, second of the second conveying rotating disc of support Dagger, the second device of rotation driving that the second conveying rotating disc of driving is rotated using the second support column as shaft, by the second conveying Culture bucket on turntable is pushed to the second pushing meanss on the second-order conveyer belt, will the corresponding first rank conveyer belt end The culture bucket at end dials to the first striking gear on the second conveying rotating disc, and the first rank target seedling leaf color of detection the Second colors detection device；

The edge of second conveying rotating disc is equipped with sliding between the second conveying rotating disc and second-order conveyer belt for culture bucket The second dynamic elasticity transition side, second conveying rotating disc are formed with the second rotation hole being inserted on second support column；Institute Stating the second device of rotation driving includes the second driven gear being placed on second support column, driving the second driven gear rotation The second driving gear, driving the second driving gear rotation the second motor；The upper end of second driven gear and described the The lower surface of two conveying rotating discs links together, and second driving gear matches with second driven gear and is engaged on one It rises；Second support column, which is equipped with, to be resisted against the second of second driven gear lower end and supports block, second driven gear And second support between block be equipped with second bearing；Second pushing meanss include being connected to second upper end of support column and court To the second fluid cylinder pressure of corresponding second-order conveyer belt, and set on the second push plate of second fluid cylinder pressure output end；Described second For the output end of fluid-pressure cylinder towards culture bucket, the second fluid cylinder pressure and the second-order conveyer belt are perpendicular；

The second-order conveyer belt includes second that the second belt body and the second belt body of driving are gone up and down Lifting drive；Second belt body includes the second driving pulley, the second driven pulley, is connected to the second main belt Second feeder motor of the second conveyer belt and driving the second driving pulley rotation between wheel and the second driven pulley；It is described Second lifting drive includes the second lifting support column of support second driving pulley and the second driven pulley, and carrying is each Second lifting support plate of the second lifting support column, and the second lifting fluid pressure that driving the second lifting support plate is gone up and down Cylinder；

First striking gear includes first stirring support column, is connected to first and stirs upper end of support column and with described the The first parallel main fluid cylinder pressure of single order conveyer belt, horizontal connection in the first main fluid cylinder pressure output end and with vertically phase The first secondary fluid cylinder pressure of the first rank conveyer belt is answered, and stirs push plate set on the first of the first secondary fluid cylinder pressure output end；

Second color detection means include being transferred to the controller above culture bucket and by image data Second color image pickup apparatus supports the second gooseneck boom of the second color image pickup apparatus, supports the second column of the second gooseneck boom；Described Two color image pickup apparatus include recess the second vault cover directed downwardly and multiple the second colored camera shootings being distributed in the second vault cover recess Head；

The terminal installation includes third rank culture unit group corresponding with the end of the second-order conveyer belt and connects Receive the terminal receiving platform for the second-order target seedling being removed；

The third rank culture unit group includes the third conveying rotating disc for carrying multiple culture buckets, incudes third conveying rotating disc Turned position and the third place sensor that position signal is transferred to the controller support the third branch of third conveying rotating disc Dagger, the third device of rotation driving that driving third conveying rotating disc is rotated using third support column as shaft, third is conveyed Culture bucket on turntable is pushed to the third pushing meanss on the terminal receiving platform, and detection second-order target seedling leaf The third color detection means of color；

The edge of the third conveying rotating disc is equipped with to be received to slide between platform for culture bucket in third conveying rotating disc and terminal Dynamic third elasticity transition side, the third conveying rotating disc are formed with the third rotation hole being inserted on the third support column；Institute Stating third device of rotation driving includes the third driven gear being placed on the third support column, driving third driven gear rotation Third driving gear, driving third driving gear rotation third motor；The upper end of the third driven gear and described the The lower surface of three conveying rotating discs links together, and the third driving gear matches with the third driven gear and is engaged on one It rises；The third support column supports block, the third driven gear equipped with the third for being resisted against third driven gear lower end And third is supported and is equipped with 3rd bearing between block；The third pushing meanss include being connected to the third upper end of support column and court To the third fluid-pressure cylinder of the terminal receiving platform, and set on the third push plate of third fluid-pressure cylinder output end；The third The output end direction culture bucket of fluid-pressure cylinder；

Second striking gear includes second stirring support column, is connected to second and stirs upper end of support column and with described the The second parallel main fluid cylinder pressure of second order conveyer belt, horizontal connection is in the second main fluid cylinder pressure output end and with vertically The second subsidiary stream body cylinder pressure of second order conveyer belt, and push plate is stirred set on the second of second subsidiary stream body cylinder pressure output end；

The third color detection means include being transferred to the controller above culture bucket and by image data Third color image pickup apparatus supports the third gooseneck boom of third color image pickup apparatus, supports the third column of third gooseneck boom；Described Three color image pickup apparatus include recess third vault cover directed downwardly and multiple third colour camera shootings being distributed in third vault cover recess Head；

During carrying out the selection of first stage color variety and second stage color variety is chosen, take root first by multiple Seedling is placed in the edge of each first conveying rotating disc after being contained respectively with culture bucket, and setting blade is yellow in the controller The shallow numerical value of color depth and yellow value region area accounting numerical value；First segment culture is carried out to rooted plantlet, after cultivating a period of time, It drives the first driving gear that the first driven gear is driven to rotate using controller control first motor, and then the first conveying is driven to turn Disk drives each culture bucket and rooted plantlet on the first conveying rotating disc to rotate around the first support column, and each bucket of cultivating passes through accordingly one by one The first color image pickup apparatus below, when cultivate bucket reach the first color image pickup apparatus lower section when, controller control first electricity Machine pause, corresponding culture bucket and rooted plantlet pause at the lower section of the first color image pickup apparatus, each first colour imagery shot pair Each blade of this rooted plantlet is shot, and image pickup signal is transferred to controller, by controller in blade and blade Yellow area identified, when yellow depth numerical value and yellow value region area accounting numerical value reach setting numerical requirements, This rooted plantlet records position by controller as the first rank target seedling, and then controller controls first motor after reforwarding Row, the first conveying rotating disc drive each culture bucket and rooted plantlet to remain in operation, where the first rank target seedling reaches the first push plate When place, this first rank target seedling is simultaneously pushed to the conveying of the first rank using the first push plate by controller control first fluid cylinder pressure starting It takes, by the first rank conveyer belt by the first rank target seedling downstream transport, completes first stage color variety and choose；When the first rank When target seedling reaches the first rank conveying end of tape, empty position and the first rank conveyer belt end of the second conveying rotating disc are controlled by controller End corresponds to, and controller, which controls the starting of the first secondary fluid cylinder pressure, makes first to stir the upstream that push plate is in the first rank target seedling, then First main fluid cylinder pressure starting drives the first secondary fluid cylinder pressure and first to stir push plate the first rank target seedling is pushed to second On the empty position of conveying rotating disc；Second segment culture, culture a period of time are carried out to the first rank target seedling on the second conveying rotating disc Afterwards, controlling second the second driving gear of motor driven using controller drives the second driven gear to rotate, and then driving second is defeated Feed turntable drives each culture bucket and the first rank target seedling on the second conveying rotating disc to rotate around the second support column, each to cultivate bucket one by one Below corresponding second color image pickup apparatus, when cultivating the lower section of bucket the second color image pickup apparatus of arrival, controller control Make the pause of the second motor, it is corresponding to cultivate bucket and the first rank target seedling pauses at the lower section of the second color image pickup apparatus, each second Colour imagery shot shoots each blade of this first rank target seedling, and image pickup signal is transferred to controller, by controller Yellow area in blade and blade is identified, when yellow depth numerical value and yellow value region area accounting numerical value reach When setting numerical requirements, this first rank target seedling records position by controller as second-order target seedling, then controller It controls the second motor to continue to run, the second conveying rotating disc drives each culture bucket and second-order target seedling to remain in operation, and works as second-order When target seedling is reached at the second push plate place, controller control second fluid cylinder pressure starts and utilizes the second push plate by this second-order Target seedling is pushed on second-order conveyer belt, by second-order conveyer belt by second-order target seedling downstream transport, completes second-order Section color variety is chosen；

When second-order target seedling reaches second-order conveying end of tape, by the empty position of controller control third conveying rotating disc Corresponding with second-order conveying end of tape, controller control second subsidiary stream body cylinder pressure, which starts, to be made second to stir push plate to be in second-order mesh The upstream of seedling is marked, then the starting of the second main fluid cylinder pressure is to drive second subsidiary stream body cylinder pressure and second to stir push plate for second-order mesh Mark seedling is pushed on the empty position of third conveying rotating disc；Third Duan Pei is carried out to second-order target seedling on third conveying rotating disc It supports, after cultivating a period of time, drives third driven gear to rotate using controller control third motor driven third driving gear, And then drive third conveying rotating disc that each culture bucket on third conveying rotating disc and second-order target seedling is driven to turn around third support column It is dynamic, it is each to cultivate bucket one by one by corresponding third color image pickup apparatus lower section, when culture bucket reaches third color image pickup apparatus When lower section, controller controls the pause of third motor, and corresponding culture bucket and second-order target seedling pause at third colour camera shooting dress The lower section set, each third colour imagery shot shoots each blade of this second-order target seedling, and image pickup signal is transferred to Controller identifies the yellow area in blade and blade by controller, when yellow depth numerical value and yellow value region When area accounting numerical value not up to sets numerical requirements, where this second-order target seedling is recorded as rejecting target seedling by controller Position, then controller control third motor continues to run, and third conveying rotating disc, which drives each culture bucket and rejects target seedling, to be continued Operating, when rejecting at target seedling arrival third push plate place, controller control third fluid-pressure cylinder is started and is pushed away using third This rejecting target seedling is pushed on terminal receiving platform by plate, is completed color variations and is rejected operation.

In the step (3), it is equipped in the controller on the software and analysis blade of identification blade and meets yellow The software of value region accounting, and carry out yellow depth numerical value and yellow numerical value set when second stage color variety is chosen Region area accounting is above first stage color variety and chooses set yellow depth numerical value and yellow value region area Accounting.

In the step (4), the multiple stem cells for obtaining second-order target seedling carry out cell culture respectively, until training Form more plants of gold lotus seedlings.

After adopting the above technical scheme, the seed selection cultivating technique of gold lotus of the invention, breaks through the cultivation of traditional roxburgh anoectochilus terminal bud Process form, by multiple whole strain balled transplantings of field roxburgh anoectochilus terminal bud plant with yellow blade into artificial greenhouse, in roxburgh anoectochilus terminal bud The plant florescence carry out artificial pollination, picked to fruit maturation, provided by artificial preliminary screening be possible to chloroaniline because Roxburgh anoectochilus terminal bud, be remarkably improved the later period screening efficiency；The embryo for obtaining the fruit of picking, embryo is placed in culture medium and is cultivated Obtain rooted plantlet；The selection of first stage color variety is carried out to multiple rooted plantlets, is selected plurality of with yellow blade Rooted plantlet as the first rank target seedling, this step can maximum probability select the roxburgh anoectochilus terminal bud with chloroaniline because of possibility；To more A first rank target seedling carries out carrying out the selection of second stage color variety after culture a period of time, select wherein blade most it is yellow extremely For a few first rank target seedling as second-order target seedling, it is only blade early period in yellow but later period that this step, which can be removed most of, The roxburgh anoectochilus terminal bud or maximum probability of blade greening have the roxburgh anoectochilus terminal bud of variation possibility, leave substantially purebred and stable gold lotus；So Afterwards second-order target seedling cultivate up to yielding positive results, and picked to fruit maturation, obtains the kind of the fruit of picking Embryo, and then go out by multiple Seed embryo cultures the gold lotus of multiple golden yellow blades；Or cell culture is carried out to second-order target seedling, Obtain the gold lotus of multiple golden yellow blades；This step can carry out big quantity breeding to the gold lotus distinct variety selected, and be convenient for A wide range of plantation and sale.Compared with prior art, the seed selection cultivating technique of gold lotus of the invention, can carry out efficient and steady Fixed gold lotus breed of variety.

Detailed description of the invention

Fig. 1 is the overlooking structure diagram of seed selection breeding apparatus；

Fig. 2 is the first angle broken section structural schematic diagram of seed selection breeding apparatus；

Fig. 3 is the second angle broken section structural schematic diagram of seed selection breeding apparatus；

Fig. 4 is the first partial structural schematic diagram of seed selection breeding apparatus；

Fig. 5 is the second partial structural diagram of seed selection breeding apparatus.

In figure:

1111- first conveyer belt main body 11111- the first driving pulley the first driven pulley of 11112- 11113- first is conveyed Band 11121- first goes up and down support column 11122- first and goes up and down the lifting fluid-pressure cylinder of support plate 11123- first

1121- the first conveying rotating disc 11211- the first elasticity transition the first support column of side 1122- 11221- first supports block 11222- first bearing 11231- the first driven gear 11232- the first driving gear 11233- first motor 1124- first is pushed Device 11241- first fluid cylinder pressure the first push plate of 11242-

1125- the first color detection means 112511- the first vault cover 11252- the first gooseneck boom the first column of 11253-

211- the second belt body 2111- the second driving pulley 2112- the second driven pulley the second conveyer belt of 2113- 2121- second goes up and down support column 2122- second and goes up and down the lifting fluid-pressure cylinder of support plate 2123- second

22- second-order culture unit group 221- the second conveying rotating disc 2211- the second elasticity transition the second support column of side 222- 2221- second supports block 2222- second bearing 2231- the second driven gear 2232- the second driving gear the second motor of 2233- It is main that 224- the second pushing meanss 2241- second fluid cylinder pressure the second push plate of 2242- 2251- first stirs support column 2252- first Fluid-pressure cylinder 2253- the first secondary fluid cylinder pressure 2254- first stirs push plate

226- the second color detection means 22611- the second vault cover 2262- the second gooseneck boom the second column of 2263-

311- third conveying rotating disc 3111- third elasticity transition side 312- third support column 3121- third supports block 3122- 3rd bearing 3131- third driven gear 3132- third driving gear 3133- third motor 314- third pushing meanss 3141- Third fluid-pressure cylinder 3142- third push plate 3151- second stirs support column 3152- the second main fluid cylinder pressure 3153- second subsidiary stream Body cylinder pressure 3154- second stirs push plate

316- third color detection means 31611- third vault cover 3162- third gooseneck boom 3163- third column

32- terminal receiving platform

10- cultivates bucket.

Specific embodiment

In order to further explain the technical solution of the present invention, being described in detail below by specific embodiment.

A kind of seed selection cultivating technique of gold lotus of the invention includes the following steps: as shown in Figs. 1-5

(1) the whole strain balled transplanting of field roxburgh anoectochilus terminal bud plant by multiple with yellow blade is into artificial greenhouse, in gold thread The lotus plant florescence carries out artificial pollination, is picked to fruit maturation, is provided and be possible to chloroaniline by artificial preliminary screening The roxburgh anoectochilus terminal bud of cause is remarkably improved the efficiency of later period screening, and can avoid extraneous uncertain factor influence, convenient for management；

(3) selection of first stage color variety is carried out to multiple rooted plantlets, selected plurality of with yellow blade Rooted plantlet as the first rank target seedling, this step can maximum probability select the roxburgh anoectochilus terminal bud with chloroaniline because of possibility；

The selection of second stage color variety is carried out after carrying out culture a period of time to multiple first rank target seedlings, is selected wherein For at least one most yellow first rank target seedling of blade as second-order target seedling, it is only blade early period that this step, which can be removed most of, There is the roxburgh anoectochilus terminal bud of variation possibility in the roxburgh anoectochilus terminal bud or maximum probability of yellow but anaphase blade greening, leave substantially purebred and steady Fixed gold lotus；

Or cell culture is carried out to second-order target seedling, obtain the gold lotus of multiple golden yellow blades；

This step can carry out big quantity breeding to the gold lotus distinct variety selected, convenient for a wide range of plantation and sale.

Preferably, between step (3) and (4), color variations is carried out and reject operation, it is certain to second-order target seedling culture Time, to yellow blade gradually tail off or blade in the gradually smaller second-order target seedling of yellow area area accounting pick Remove, only leave yellow blade do not tail off or blade in the second-order target seedling that does not become smaller of yellow area area accounting.It is carrying out greatly Before batch is bred, it is blade early period in yellow but the gold thread of anaphase blade greening that this step, which can further remove remaining only, Lotus or maximum probability have the roxburgh anoectochilus terminal bud of variation possibility, leave purebred and stable gold lotus, it is ensured that gold lotus quality.

Preferably, in step (2), operation is followed the steps below,

Embryo sowing is cultivated in germination medium, obtains protocorm by S1, the embryo for obtaining the fruit picked； Germination medium concretely MS, sucrose 33-34g/L, AC2g/L, and agar 7-8g/L is added, pH is adjusted to 5.7-5.9；

S2, protocorm is transferred in differential medium and is cultivated, obtain sprout；Differential medium concretely MS, Sucrose 33-34g/L, AC2g/L, potato 230-240g/L, and agar 7-8g/L is added, pH is adjusted to 5.7-5.9；

S3, sprout is transferred in the strong culture medium of increasing and is cultivated, obtain strong sprout；Increase strong culture medium concretely MS, sugarcane Sugared 33-34g/L, AC2g/L, bananas juice 124-128g/L, and agar 7-8g/L is added, pH is adjusted to 5.7-5.9；

S4, strong sprout is transferred in root media and is cultivated, obtain rooted plantlet；Root media concretely 1/ 2MS, sucrose 27-28g/L, NAA1mg/L, and the semisolids such as agar, active carbon or solid matter is added, pH is adjusted to 5.7- 5.9；

S5, rooted plantlet is taken out one by one and is transplanted, specifically transplanting is into culture bucket 10.

Preferably, in step sl, first dark at a temperature of humidity 60-65%, 24-25 DEG C of temperature to train 12-14 days, then exist Under the conditions of intensity of illumination 1000-1500Lux, culture 23-25 days is carried out with daily 12 hours light application times；Sprout into protocorm Probability reach 99% or more；

In step s 2, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 1500-2500Lux, with every Its 12 hours light application time carries out culture 23-25 days；Sprout growth is substantially good, and accidental bad sprout is essentially 0；

In step s3, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 2000-3000Lux, with every Its 12 hours light application time carries out culture 30 days.Strong sprout growth is substantially good, and accidental bad strong sprout is essentially 0.

Preferably, in step (3), the selection of first stage color variety and second stage are carried out using seed selection breeding apparatus Color variety is chosen；

Preferably, seed selection breeding apparatus includes the first rank device for carrying out the selection of first stage color variety, carries out second The second-order device that stage color variety is chosen receives the terminal installation of second-order target seedling, and to the first rank device, second The controller that rank device and terminal installation are controlled；

Preferably, the first rank device includes multiple parallel the first rank culture unit rows；

Preferably, the first rank culture unit row includes the first rank conveyer belt and multiple along the of the arrangement of the first rank conveyer belt Single order culture unit group；Each first rank conveyer belt is parallel；

Preferably, the first rank culture unit group includes the first conveying rotating discs 1121 for carrying multiple culture buckets 10, induction the One conveying rotating disc, 1121 turned position and the first position sensor that position signal is transferred to controller, the first conveying of support turn First support column 1122 of disk 1121 drives that the first conveying rotating disc 1121 is that shaft is rotated with the first support column 1122 One device of rotation driving, first that the culture bucket 10 on the first conveying rotating disc 1121 is pushed on the first rank conveyer belt, which pushes, to be filled Set 1124, and the first color detection means 1125 of detection rooted plantlet leaf color；

Preferably, the edge of the first conveying rotating disc 1121 is equipped with for culture bucket 10 in the first conveying rotating disc 1121 and the first rank The the first elasticity transition side 11211 slided between conveyer belt, the first conveying rotating disc 1121, which is formed with, is inserted in the first support column 1122 On the first rotation hole；First device of rotation driving includes the first driven gear 11231 being placed on the first support column 1122, is driven First driving gear 11232 of dynamic first driven gear 11231 rotation, the first electricity of driving the first driving gear 11232 rotation Machine 11233；The upper end of first driven gear 11231 and the lower surface of the first conveying rotating disc 1121 link together, and first actively Gear 11232 is matched with the first driven gear 11231 to be meshed together；First support column 1122 is driven equipped with being resisted against first The first of 11231 lower end of gear supports block 11221, and the first driven gear 11231 and first, which are supported, is equipped with first between block 11221 Bearing 11222；First pushing meanss 1124 include being connected to 1122 upper end of the first support column and towards corresponding first rank conveyer belt First fluid cylinder pressure 11241, and the first push plate 11242 set on 11241 output end of first fluid cylinder pressure；First fluid cylinder pressure For 11241 output end towards culture bucket 10, first fluid cylinder pressure 11241 and the first rank conveyer belt are perpendicular；

Preferably, the first rank conveyer belt includes that 11113 main body 1111 of first conveyer belt and driving first conveyer belt 11113 are main The first lifting drive that body 1111 is gone up and down；11113 main body 1111 of first conveyer belt includes the first driving pulley 11111, the first driven pulley 11112, be connected between the first driving pulley 11111 and the first driven pulley 11112 first Conveyer belt 11113 and the first feeder motor of driving the first driving pulley 11111 rotation；First lifting drive includes The the first lifting support column 11121 for supporting the first driving pulley 11111 and the first driven pulley 11112, carries each first lifting First lifting support plate 11122 of support column 11121, and driving first go up and down first liter that support plate 11122 is gone up and down Fluid-pressure cylinder 11123 drops；

Preferably, the first color detection means 1125 include that in 10 top of culture bucket and image data is transferred to control First color image pickup apparatus of device supports the first gooseneck boom 11252 of the first color image pickup apparatus, the first gooseneck boom 11252 of support First column 11253；First color image pickup apparatus includes recess the first vault cover 112511 directed downwardly and multiple is distributed in the first vault Cover the first colour imagery shot in 112511 recesses；

Preferably, second-order device includes perpendicular second-order conveyer belt and multiple edges with each first rank conveyer belt The arrangement of second-order conveyer belt and second-order culture unit group 22 corresponding with the end of each first rank conveyer belt；

Preferably, second-order culture unit group 22 includes the second conveying rotating discs 221 for carrying multiple culture buckets 10, induction the Two conveying rotating discs, 221 turned position and the second position sensor that position signal is transferred to controller, the second conveying of support turn Second support column 222 of disk 221 drives second turn that the second conveying rotating disc 221 is rotated with the second support column 222 for shaft Dynamic driving device, is pushed to the second pushing meanss on second-order conveyer belt for the culture bucket 10 on the second conveying rotating disc 221 224, the culture bucket 10 of corresponding first rank conveying end of tape is dialed into the striking gear on the second conveying rotating disc 221, Yi Jijian Survey the second color detection means of the first rank target seedling leaf color；

Preferably, the edge of the second conveying rotating disc 221 is equipped with defeated in the second conveying rotating disc 221 and second-order for culture bucket 10 Send the second elasticity transition side 2211 for sliding between band, the second conveying rotating disc 221 is formed with the be inserted on the second support column 222 Two rotation holes；Second device of rotation driving includes the second driven gear 2231 being placed on the second support column 222, driving second from The second driving gear 2232 that moving gear 2231 rotates, the second motor 2233 of driving the second driving gear 2232 rotation；Second The upper end of driven gear 2231 and the lower surface of the second conveying rotating disc 221 link together, the second driving gear 2232 and second Driven gear 2231, which matches, to be meshed together；Second support column 222, which is equipped with, is resisted against the of 2231 lower end of the second driven gear Two support block 2221, and the second driven gear 2231 and second, which are supported, is equipped with second bearing 2222 between block 2221；Second pushes dress 224 are set including being connected to 222 upper end of the second support column and towards the second fluid cylinder pressure 2241 of corresponding second-order conveyer belt, and is set In the second push plate 2242 of 2241 output end of second fluid cylinder pressure；The output end direction culture bucket 10 of second fluid cylinder pressure 2241, Second fluid cylinder pressure 2241 and second-order conveyer belt are perpendicular；

Preferably, second-order conveyer belt includes 2113 main body 211 of the second conveyer belt and driving 2113 main body of the second conveyer belt 211 the second lifting drives gone up and down；Second conveyer belt, 2113 main body 211 includes the second driving pulley 2111, second Driven pulley 2112, the second conveyer belt 2113 being connected between the second driving pulley 2111 and the second driven pulley 2112, with And the second feeder motor of driving the second driving pulley 2111 rotation；Second lifting drive includes the second driving pulley of support 2111 and second driven pulley 2112 second lifting support column 2121, carry it is each second lifting support column 2121 second lifting Support plate 2122, and the second lifting fluid-pressure cylinder 2123 that driving the second lifting support plate 2122 is gone up and down；

Preferably, the first striking gear stirs support column 2251 including first, is connected to first and stirs on support column 2251 It holds and the first main fluid cylinder pressure 2252 parallel with the first rank conveyer belt, horizontal connection is exported in the first main fluid cylinder pressure 2252 Hold and to the first secondary fluid cylinder pressure 2253 of vertically corresponding first rank conveyer belt, and be set to the first secondary fluid cylinder pressure 2253 The first of output end stirs push plate 2254；

Preferably, the second color detection means 226 include that in 10 top of culture bucket and image data is transferred to control Second color image pickup apparatus of device, supports the second gooseneck boom 2262 of the second color image pickup apparatus, and the of the second gooseneck boom 2262 of support Two columns 2263；Second color image pickup apparatus includes recess the second vault cover 22611 directed downwardly and multiple is distributed in the second vault cover The second colour imagery shot in 22611 recesses；

Preferably, terminal installation includes third rank culture unit group corresponding with the end of second-order conveyer belt and reception The terminal receiving platform 32 for the second-order target seedling being removed；

Preferably, third rank culture unit group includes the third conveying rotating disc 311 for carrying multiple culture buckets 10, incudes third 311 turned position of conveying rotating disc and the third place sensor that position signal is transferred to controller support third conveying rotating disc 311 third support column 312, the third rotation for driving third conveying rotating disc 311 to be rotated with third support column 312 for shaft Culture bucket 10 on third conveying rotating disc 311 is pushed to the third pushing meanss on terminal receiving platform 32 by driving device 314, and the third color detection means of detection second-order target seedling leaf color；

Preferably, the edge of third conveying rotating disc 311, which is equipped with, receives for culture bucket 10 in third conveying rotating disc 311 and terminal The third elasticity transition side 3111 slided between platform, third conveying rotating disc 311 are formed be inserted on third support column 312 Three rotation holes；Third device of rotation driving includes the third driven gear 3131 being placed on third support column 312, driving third from The third driving gear 3132 that moving gear 3131 rotates, the third motor 3133 that driving third driving gear 3132 rotates；Third The upper end of driven gear 3131 and the lower surface of third conveying rotating disc 311 link together, third driving gear 3132 and third Driven gear 3131, which matches, to be meshed together；Third support column 312, which is equipped with, is resisted against the of 3131 lower end of third driven gear Three support block 3121, and third driven gear 3131 and third, which are supported, is equipped with 3rd bearing 3122 between block 3121；Third pushes dress Setting 314 includes being connected to 312 upper end of third support column and the third fluid-pressure cylinder 3141 towards terminal receiving platform 32, and be set to The third push plate 3142 of 3141 output end of third fluid-pressure cylinder；The output end direction culture bucket 10 of third fluid-pressure cylinder 3141；

Preferably, the second striking gear stirs support column 3151 including second, is connected to second and stirs on support column 3151 It holds and the second main fluid cylinder pressure 3152 parallel with second-order conveyer belt, horizontal connection is exported in the second main fluid cylinder pressure 3152 End and the second subsidiary stream body cylinder pressure 3153 with vertically second-order conveyer belt, and be set to second subsidiary stream body cylinder pressure 3153 and export The second of end stirs push plate 3154；

Preferably, third color detection means 316 include that in 10 top of culture bucket and image data is transferred to control The third color image pickup apparatus of device, supports the third gooseneck boom 3162 of third color image pickup apparatus, and the of support third gooseneck boom 3162 Three columns 3163；Third color image pickup apparatus includes recess third vault cover 31611 directed downwardly and multiple is distributed in third vault cover Third colour imagery shot in 31611 recesses；

Preferably, during carrying out the selection of first stage color variety and second stage color variety is chosen, first will Multiple rooted plantlets are placed in the edge of each first conveying rotating disc 1121 after being contained respectively with culture bucket 10, and are controlling Blade yellow depth numerical value and yellow value region area accounting numerical value are set in device；First segment culture is carried out to rooted plantlet, After cultivating a period of time, the first driving gear 11232 is driven to drive the first driven tooth using controller control first motor 11233 11231 rotation of wheel, and then the first conveying rotating disc 1121 is driven to drive each culture bucket 10 on the first conveying rotating disc 1121 and take root Seedling rotates around the first support column 1122, each to cultivate bucket 10 one by one by corresponding first color image pickup apparatus lower section, works as culture When bucket 10 reaches the lower section of the first color image pickup apparatus, controller controls first motor 11233 and suspends, corresponding to cultivate 10 He of bucket Rooted plantlet pauses at the lower section of the first color image pickup apparatus, and each first colour imagery shot carries out each blade of this rooted plantlet Shooting, and image pickup signal is transferred to controller, the yellow area in blade and blade is identified by controller, works as yellow When depth numerical value and yellow value region area accounting numerical value reach setting numerical requirements, this rooted plantlet is as the first rank target Seedling records position by controller, and then controller control first motor 11233 continues to run, 1121 band of the first conveying rotating disc It moves each culture bucket 10 and rooted plantlet remains in operation, when where the first rank target seedling reaching the first push plate 11242, controller This first rank target seedling is simultaneously pushed to the conveying of the first rank using the first push plate 11242 by the starting of control first fluid cylinder pressure 11241 It takes, by the first rank conveyer belt by the first rank target seedling downstream transport, completes first stage color variety and choose；When the first rank When target seedling reaches the first rank conveying end of tape, the empty position of the second conveying rotating disc 221 is controlled by controller and the first rank conveys End of tape is corresponding, and controller, which controls the starting of the first secondary fluid cylinder pressure 2253 and makes first to stir push plate 2254, is in the first rank target seedling Upstream, then the first main fluid cylinder pressure 2252 starting come drive the first secondary fluid cylinder pressure 2253 and first stir push plate 2254 will First rank target seedling is pushed on the empty position of the second conveying rotating disc 221；To the first rank target seedling on the second conveying rotating disc 221 Second segment culture is carried out, after cultivating a period of time, the second motor 2233 is controlled using controller and drives the second driving gear 2232 It drives the second driven gear 2231 to rotate, and then the second conveying rotating disc 221 is driven to drive each culture on the second conveying rotating disc 221 Bucket 10 and the first rank target seedling are rotated around the second support column 222, each to cultivate bucket 10 one by one by the corresponding second colored camera shooting dress Lower section is set, when cultivating the lower section of the second color image pickup apparatus of arrival of bucket 10, controller controls the pause of the second motor 2233, accordingly Culture bucket 10 and the first rank target seedling pause at the lower section of the second color image pickup apparatus, each second colour imagery shot to this first Each blade of rank target seedling is shot, and image pickup signal is transferred to controller, by controller to the Huang in blade and blade Color region identified, when yellow depth numerical value and yellow value region area accounting numerical value reach setting numerical requirements, this First rank target seedling records position by controller as second-order target seedling, then controller control the second motor 2233 after Reforwarding row, the second conveying rotating disc 221 drive each culture bucket 10 and second-order target seedling to remain in operation, when second-order target seedling reaches When where the second push plate 2242, controller control second fluid cylinder pressure 2241 start and using the second push plate 2242 by this second Rank target seedling is pushed on second-order conveyer belt, by second-order conveyer belt by second-order target seedling downstream transport, completes second Stage color variety is chosen；

Preferably, when second-order target seedling reaches second-order conveying end of tape, third conveying rotating disc is controlled by controller 311 empty position is corresponding with second-order conveying end of tape, and the controller control starting of second subsidiary stream body cylinder pressure 3153 makes second to stir Push plate 3154 is in the upstream of second-order target seedling, and then the starting of the second main fluid cylinder pressure 3152 is to drive second subsidiary stream body cylinder pressure 3153 and second stir push plate 3154 second-order target seedling are pushed on the empty position of third conveying rotating disc 311；It is defeated in third Three stage cultivation is carried out to second-order target seedling in feed turntable 311, after cultivating a period of time, controls third motor using controller 3133 driving third driving gears 3132 drive third driven gear 3131 to rotate, and then third conveying rotating disc 311 is driven to drive Each culture bucket 10 and second-order target seedling on third conveying rotating disc 311 are rotated around third support column 312, each to cultivate bucket 10 one by one Below corresponding third color image pickup apparatus, when cultivating the lower section of the arrival third color image pickup apparatus of bucket 10, controller It controls third motor 3133 to suspend, corresponding culture bucket 10 and second-order target seedling pause under third color image pickup apparatus Side, each third colour imagery shot shoots each blade of this second-order target seedling, and image pickup signal is transferred to controller, The yellow area in blade and blade is identified by controller, when yellow depth numerical value and yellow value region area accounting When numerical value not up to sets numerical requirements, this second-order target seedling records position by controller as rejecting target seedling, so Controller control third motor 3133 continues to run afterwards, and third conveying rotating disc 311 drives each culture bucket 10 and rejects target Miao Ji Reforwarding turns, and when where rejecting target seedling and reach third push plate 3142, controller controls the starting of third fluid-pressure cylinder 3141 simultaneously This rejecting target seedling is pushed on terminal receiving platform 32 using third push plate 3142, color variations is completed and rejects operation.

Preferably, it in step (3), is equipped in controller on the software and analysis blade of identification blade and meets yellow number It is worth the software of region accounting, and carries out yellow depth numerical value and yellow numerical value area set when second stage color variety is chosen Domain area accounting is above the selection of first stage color variety set yellow depth numerical value and yellow value region area and accounts for Than.

Preferably, in step (4), the multiple stem cells for obtaining second-order target seedling carry out cell culture respectively, directly To being trained more plants of gold lotus seedlings.

In order to improve the stability of equilibrium of support and lifting, it is preferable that the balanced branch of multiple first lifting fluid-pressure cylinders 11123 It supports in the lower section of the first lifting support plate 11122, multiple second lifting 2123 uniform supports of fluid-pressure cylinder are in the second lifting support The lower section of plate 2122, multiple third lifting fluid-pressure cylinder uniform supports are in the lower section of third lifting support plate.

Preferably, multiple first colour imagery shots are uniformly distributed in the recess of the first vault cover 112511, and multiple second is color Color camera is uniformly distributed in the recess of the second vault cover 22611, and multiple third colour imagery shots are uniformly distributed in third vault cover In 31611 recess.This structure can Multi-angle omnibearing roxburgh anoectochilus terminal bud blade is shot, more comprehensively.And adjustable gold Line lotus extend into the depth in recess, and then can carry out stereoscopic shooting to roxburgh anoectochilus terminal bud, and data are more comprehensive.

Preferably, the first elasticity transition side 11211 includes multiple the first steel around the arrangement of 1121 edge of the first conveying rotating disc Plate, the second elasticity transition side 2211 include multiple the second steel plates around the arrangement of 221 edge of the second conveying rotating disc, third elasticity transition Side 3111 includes multiple third steel plates around the arrangement of 311 edge of third conveying rotating disc.The present invention is each defeated in practical work process Send with outer end in contact with each steel plate after rising, each steel plate can carry out flexible deformation slightly and be buffered, and with it is corresponding Conveyer belt fits closely together, and culture bucket 10 can slide up and down under the transition guiding role of corresponding steel plate, realizes position Set variation.When conveyer belt is contacted with corresponding elasticity transition side, corresponding conveying rotating disc shuts down, only when conveyer belt to Under move away elasticity transition side after, corresponding conveying rotating disc can just operate；And the operating of conveyer belt is also only corresponding Conveyer belt separated with elasticity transition side after can just operate；Conveyer belt and elasticity transition side is avoided to generate frictionally damage.

In order to ensure the stability of each fluid-pressure cylinder work, it is preferable that the upper end of the first support column 1122 is equipped with support the First supporting block of one fluid-pressure cylinder 11241, the upper end of the second support column 222 are equipped with the second of support second fluid cylinder pressure 2241 Supporting block, the upper end of third support column 312 are equipped with the third supporting block of support third fluid-pressure cylinder 3141；First stirs support column 2251 upper end, which is equipped with, supports the first of the first main fluid cylinder pressure 2252 to stir supporting block, and second stirs the upper end of support column 3151 Second equipped with the second main fluid cylinder pressure 3152 of support stirs supporting block.Each fluid-pressure cylinder concretely cylinder or oil cylinder.

Preferably, seed selection breeding apparatus further includes carrying the first support column 1122, the second support column 222, third support column 312, first stir that support column 2251, second to stir support column 3151, the first column 11253, the second column 2263 and third vertical The loading plate of column 3163.

Preferably, can each 10 position of culture bucket be numbered, controller is sentenced by the rotational angle of each motor Break it is each culture bucket 10 position.

Product form of the invention is not limited to this case diagram and embodiment, anyone carries out the appropriate of similar thinking to it Changes or modifications all should be regarded as not departing from patent category of the invention.

Claims

1. a kind of seed selection cultivating technique of gold lotus, which comprises the steps of:

(1) the whole strain balled transplanting of field roxburgh anoectochilus terminal bud plant by multiple with yellow blade is planted into artificial greenhouse in roxburgh anoectochilus terminal bud The strain florescence carries out artificial pollination, is picked to fruit maturation；

(3) selection of first stage color variety is carried out to multiple rooted plantlets, selects plurality of taking root with yellow blade Seedling is as the first rank target seedling；

The selection of second stage color variety is carried out after carrying out culture a period of time to multiple first rank target seedlings, selects wherein blade Most at least one yellow first rank target seedling is as second-order target seedling；

(4) then second-order target seedling cultivate up to yielding positive results, and picked to fruit maturation, obtain picking The embryo of fruit, and then go out by multiple Seed embryo cultures the gold lotus of multiple golden yellow blades；

2. a kind of seed selection cultivating technique of gold lotus according to claim 1, it is characterised in that: in the step (3) and (4) between, color variations is carried out and reject operation, to the second-order target seedling culture certain time, yellow blade is gradually become The gradually smaller second-order target seedling of yellow area area accounting is rejected less or in blade, is only left yellow blade and is not tailed off Or the second-order target seedling that yellow area area accounting does not become smaller in blade.

3. a kind of seed selection cultivating technique of gold lotus according to claim 2, it is characterised in that: in the step (2), Operation is followed the steps below,

S5, rooted plantlet is taken out one by one and is transplanted.

4. a kind of seed selection cultivating technique of gold lotus according to claim 3, it is characterised in that: in the step S1, It is first dark at a temperature of humidity 60-65%, 24-25 DEG C of temperature to train 12-14 days, then in intensity of illumination 1000-1500Lux condition Under, culture 23-25 days is carried out with daily 12 hours light application times；

In the step S2, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 1500-2500Lux, with every Its 12 hours light application time carries out culture 23-25 days；

In the step S3, under the conditions of humidity 60-65%, 24-25 DEG C of temperature, intensity of illumination 2000-3000Lux, with every Its 12 hours light application time carries out culture 30 days.

5. a kind of seed selection cultivating technique of gold lotus according to claim 4, it is characterised in that: in the step (3), First stage color variety is carried out using seed selection breeding apparatus to choose and the selection of second stage color variety；

The seed selection breeding apparatus includes the first rank device for carrying out the selection of first stage color variety, carries out second stage color Kind choose second-order device, receive second-order target seedling terminal installation, and to the first rank device, second-order device and The controller that terminal installation is controlled；

The first rank culture unit row includes the first rank conveyer belt and multiple the first rank cultures along the arrangement of the first rank conveyer belt Unit group；Each first rank conveyer belt is parallel；

The first rank culture unit group includes the first conveying rotating disc for carrying multiple culture buckets, induction the first conveying rotating disc rotation Position and the first position sensor that position signal is transferred to the controller, the first support of the first conveying rotating disc of support Column, the first device of rotation driving that the first conveying rotating disc of driving is rotated using the first support column as shaft turn the first conveying Culture bucket on disk is pushed to the first pushing meanss on the first rank conveyer belt, and detects rooted plantlet leaf color First color detection means；

The edge of first conveying rotating disc is equipped with to be slided between the first conveying rotating disc and the first rank conveyer belt for culture bucket First elasticity transition side, first conveying rotating disc are formed with the first rotation hole being inserted on first support column；Described One device of rotation driving includes the first driven gear being placed on first support column, and the of driving the first driven gear rotation One driving gear, the first motor of driving the first driving gear rotation；The upper end of first driven gear and described first defeated The lower surface of feed turntable links together, and first driving gear is matched with first driven gear to be meshed together； First support column, which is equipped with, to be resisted against the first of first driven gear lower end and supports block, first driven gear and the One supports between block equipped with first bearing；First pushing meanss include being connected to first upper end of support column and towards phase Answer the first fluid cylinder pressure of the first rank conveyer belt, and the first push plate set on first fluid cylinder pressure output end；The first fluid For the output end of cylinder pressure towards culture bucket, the first fluid cylinder pressure and the first rank conveyer belt are perpendicular；

The first rank conveyer belt includes the first lifting that first conveyer belt main body and driving first conveyer belt main body are gone up and down Driving device；The first conveyer belt main body include the first driving pulley, the first driven pulley, be connected to the first driving pulley and First feeder motor of first conveyer belt and driving the first driving pulley rotation between the first driven pulley；Described first Lifting drive includes the first lifting support column of support first driving pulley and the first driven pulley, carrying each first Go up and down the first lifting support plate of support column, and the first lifting fluid-pressure cylinder that driving the first lifting support plate is gone up and down；

First color detection means include being transferred to the first of the controller above culture bucket and by image data Color image pickup apparatus supports the first gooseneck boom of the first color image pickup apparatus, supports the first column of the first gooseneck boom；Described first is color Color photographic device includes recess the first vault cover directed downwardly and multiple the first colour imagery shots being distributed in the first vault cover recess；

The second-order device includes the second-order conveyer belt perpendicular with each first rank conveyer belt and multiple along second The arrangement of rank conveyer belt and second-order culture unit group corresponding with the end of each first rank conveyer belt；

The second-order culture unit group includes the second conveying rotating disc for carrying multiple culture buckets, induction the second conveying rotating disc rotation Position and the second position sensor that position signal is transferred to the controller, the second support of the second conveying rotating disc of support Column, the second device of rotation driving that the second conveying rotating disc of driving is rotated using the second support column as shaft turn the second conveying Culture bucket on disk is pushed to the second pushing meanss on the second-order conveyer belt, and corresponding first rank is conveyed end of tape Culture bucket dial to the first striking gear on the second conveying rotating disc, and the first rank target seedling leaf color of detection second Color detection means；

The edge of second conveying rotating disc is equipped with to be slided between the second conveying rotating disc and second-order conveyer belt for culture bucket Second elasticity transition side, second conveying rotating disc are formed with the second rotation hole being inserted on second support column；Described Two device of rotation driving include the second driven gear being placed on second support column, and the of driving the second driven gear rotation Two driving gears, the second motor of driving the second driving gear rotation；The upper end of second driven gear and described second defeated The lower surface of feed turntable links together, and second driving gear is matched with second driven gear to be meshed together； Second support column, which is equipped with, to be resisted against the second of second driven gear lower end and supports block, second driven gear and the Two support between block equipped with second bearing；Second pushing meanss include being connected to second upper end of support column and towards phase Answer the second fluid cylinder pressure of second-order conveyer belt, and the second push plate set on second fluid cylinder pressure output end；The second fluid For the output end of cylinder pressure towards culture bucket, the second fluid cylinder pressure and the second-order conveyer belt are perpendicular；

The second-order conveyer belt includes the second lifting that the second belt body and the second belt body of driving are gone up and down Driving device；Second belt body include the second driving pulley, the second driven pulley, be connected to the second driving pulley and Second feeder motor of the second conveyer belt and driving the second driving pulley rotation between the second driven pulley；Described second Lifting drive includes the second lifting support column of support second driving pulley and the second driven pulley, carrying each second Go up and down the second lifting support plate of support column, and the second lifting fluid-pressure cylinder that driving the second lifting support plate is gone up and down；

First striking gear includes first stirring support column, be connected to first stir upper end of support column and with first rank The first parallel main fluid cylinder pressure of conveyer belt, horizontal connection is in the first main fluid cylinder pressure output end and to vertically corresponding First secondary fluid cylinder pressure of single order conveyer belt, and push plate is stirred set on the first of the first secondary fluid cylinder pressure output end；

Second color detection means include being transferred to the second of the controller above culture bucket and by image data Color image pickup apparatus supports the second gooseneck boom of the second color image pickup apparatus, supports the second column of the second gooseneck boom；Described second is color Color photographic device includes recess the second vault cover directed downwardly and multiple the second colour imagery shots being distributed in the second vault cover recess；

The terminal installation includes third rank culture unit group corresponding with the end of the second-order conveyer belt and receives quilt The terminal receiving platform of the second-order target seedling of rejecting；

The third rank culture unit group includes the third conveying rotating disc for carrying multiple culture buckets, induction third conveying rotating disc rotation Position and the third place sensor that position signal is transferred to the controller, the third support of support third conveying rotating disc Column, the third device of rotation driving that driving third conveying rotating disc is rotated using third support column as shaft turn third conveying Culture bucket on disk is pushed to the third pushing meanss on the terminal receiving platform, and detection second-order target seedling leaf face The third color detection means of color；

The edge of the third conveying rotating disc is equipped with for culture bucket to be received to slide between platform in third conveying rotating disc and terminal Third elasticity transition side, the third conveying rotating disc are formed with the third rotation hole being inserted on the third support column；Described Three device of rotation driving include the third driven gear being placed on the third support column, and the of driving third driven gear rotation Three driving gears, the third motor of driving third driving gear rotation；The upper end of the third driven gear and the third are defeated The lower surface of feed turntable links together, and the third driving gear is matched with the third driven gear to be meshed together； The third support column, which is equipped with, to be resisted against the third of third driven gear lower end and supports block, the third driven gear and the Three support between block equipped with 3rd bearing；The third pushing meanss include being connected to the third upper end of support column and towards institute State the third fluid-pressure cylinder of terminal receiving platform, and the third push plate set on third fluid-pressure cylinder output end；The third fluid The output end direction culture bucket of cylinder pressure；

Second striking gear includes second stirring support column, be connected to second stir upper end of support column and with the second-order The second parallel main fluid cylinder pressure of conveyer belt, horizontal connection in the second main fluid cylinder pressure output end and with vertically second-order The second subsidiary stream body cylinder pressure of conveyer belt, and push plate is stirred set on the second of second subsidiary stream body cylinder pressure output end；

The third color detection means include being in above culture bucket and being transferred to image data the third of the controller Color image pickup apparatus supports the third gooseneck boom of third color image pickup apparatus, supports the third column of third gooseneck boom；The third is color Color photographic device includes recess third vault cover directed downwardly and multiple third colour imagery shots being distributed in third vault cover recess；

During carrying out the selection of first stage color variety and second stage color variety is chosen, first by multiple rooted plantlets The edge of each first conveying rotating disc is placed in after being contained respectively with culture bucket, and setting blade yellow is deep in the controller Shallow numerical value and yellow value region area accounting numerical value；First segment culture is carried out to rooted plantlet, after cultivating a period of time, is utilized Controller controls first motor and the first driving gear is driven to drive the rotation of the first driven gear, and then drives the first conveying rotating disc band Each culture bucket and rooted plantlet on dynamic first conveying rotating disc rotates around the first support column, and each culture bucket passes through corresponding the one by one Below one color image pickup apparatus, when cultivating the lower section of bucket the first color image pickup apparatus of arrival, it is temporary that controller controls first motor Stop, cultivates bucket accordingly and rooted plantlet pauses at the lower section of the first color image pickup apparatus, each first colour imagery shot is to this life Each blade of root seedling is shot, and image pickup signal is transferred to controller, by controller to the yellow in blade and blade Region identified, when yellow depth numerical value and yellow value region area accounting numerical value reach setting numerical requirements, this life Root seedling records position by controller as the first rank target seedling, and then controller control first motor continues to run, the One conveying rotating disc drives each culture bucket and rooted plantlet to remain in operation, when the first rank target seedling reaches at the first push plate place, Controller control first fluid cylinder pressure is started and this first rank target seedling is pushed on the first rank conveyer belt using the first push plate, By the first rank conveyer belt by the first rank target seedling downstream transport, completes first stage color variety and choose；When the first rank target When seedling reaches the first rank conveying end of tape, the empty position of the second conveying rotating disc is controlled by controller and the first rank conveys end of tape pair It answers, controller, which controls the starting of the first secondary fluid cylinder pressure, makes first to stir the upstream that push plate is in the first rank target seedling, and then first Main fluid cylinder pressure starting drives the first secondary fluid cylinder pressure and first to stir push plate the first rank target seedling is pushed to the second conveying On the empty position of turntable；Second segment culture is carried out to the first rank target seedling on the second conveying rotating disc, after cultivating a period of time, benefit Second the second driving gear of motor driven is controlled with controller and drives the rotation of the second driven gear, and then drives the second conveying rotating disc Each culture bucket and the first rank target seedling on the second conveying rotating disc is driven to rotate around the second support column, each bucket of cultivating passes through phase one by one Below the second color image pickup apparatus answered, when cultivating the lower section of bucket the second color image pickup apparatus of arrival, controller control second Motor pause, corresponding culture bucket and the first rank target seedling pause at the lower section of the second color image pickup apparatus, and each second colour is taken the photograph As each blade of the head to this first rank target seedling is shot, and image pickup signal is transferred to controller, by controller to blade And the yellow area in blade is identified, when yellow depth numerical value and yellow value region area accounting numerical value reach setting number When value requires, this first rank target seedling records position by controller as second-order target seedling, then controller control the Two motors continue to run, and the second conveying rotating disc drives each culture bucket and second-order target seedling to remain in operation, when second-order target seedling When reaching at the second push plate place, controller control second fluid cylinder pressure starts and utilizes the second push plate by this second-order target seedling It is pushed on second-order conveyer belt, by second-order conveyer belt by second-order target seedling downstream transport, completes second stage color Kind is chosen；

When second-order target seedling reaches second-order conveying end of tape, by the empty position and the of controller control third conveying rotating disc It is corresponding that second order conveys end of tape, and controller control second subsidiary stream body cylinder pressure starting makes second to stir push plate to be in second-order target seedling Upstream, then the second main fluid cylinder pressure starting to drive second subsidiary stream body cylinder pressure and second to stir push plate for second-order target seedling It is pushed on the empty position of third conveying rotating disc；Three stage cultivation, training are carried out to second-order target seedling on third conveying rotating disc After supporting a period of time, third driven gear is driven to rotate using controller control third motor driven third driving gear, in turn Drive third conveying rotating disc that each culture bucket on third conveying rotating disc and second-order target seedling is driven to rotate around third support column, respectively Bucket is cultivated one by one by corresponding third color image pickup apparatus lower section, when culture bucket reaches the lower section of third color image pickup apparatus When, controller controls the pause of third motor, and corresponding culture bucket and second-order target seedling pause at third color image pickup apparatus Lower section, each third colour imagery shot shoots each blade of this second-order target seedling, and image pickup signal is transferred to control Device identifies the yellow area in blade and blade by controller, when yellow depth numerical value and yellow value region area When accounting numerical value not up to sets numerical requirements, this second-order target seedling is in place by controller record institute as target seedling is rejected It sets, then controller control third motor continues to run, and third conveying rotating disc drives each culture bucket and rejects target Miao Ji reforwarding Turn, when rejecting at target seedling arrival third push plate place, controller control third fluid-pressure cylinder starts and utilizes third push plate This rejecting target seedling is pushed on terminal receiving platform, color variations is completed and rejects operation.

6. a kind of seed selection cultivating technique of gold lotus according to claim 5, it is characterised in that: in the step (3), It is equipped in the controller on the software and analysis blade of identification blade and meets the software of yellow value region accounting, and carried out Second stage color variety yellow depth numerical value and yellow value region area accounting set when choosing are above the first rank Section color variety chooses set yellow depth numerical value and yellow value region area accounting.

7. a kind of seed selection cultivating technique of gold lotus according to claim 6, it is characterised in that: in the step (4), The multiple stem cells for obtaining second-order target seedling carry out cell culture respectively, until being trained more plants of gold lotus seedlings.