CN1095543C - All wavelength surface plasma excimer resonance photochemical sensor - Google Patents
All wavelength surface plasma excimer resonance photochemical sensor Download PDFInfo
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- CN1095543C CN1095543C CN 98115735 CN98115735A CN1095543C CN 1095543 C CN1095543 C CN 1095543C CN 98115735 CN98115735 CN 98115735 CN 98115735 A CN98115735 A CN 98115735A CN 1095543 C CN1095543 C CN 1095543C
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Abstract
The present invention belongs to a photochemical sensor constructed by the excimer resonance phenomenon of surface plasma. A light source (1) of continuous wavelength is adopted to make incident light rays generate complete reflection on an interface of a prism (3) and a circulation pool (11) of injection samples; a detector (6) is used for detecting the intensity of reflecting light; a computer (20) supplies a spectrogram for finishing quantitative analysis on the samples. Consequently, a heavy rotary platform and an accurate angle measuring system are omitted. The prism (3) and the circulation pool (11) are sealed by a rubber washer. Consequently, a sensing element can be used repeatedly. The present invention has the characteristics of large researching substance range, accurate measurement, good stability, quick response, convenient operation, low cost, etc.
Description
The invention belongs to a kind of photochemistry sensor-based system, particularly utilize the photochemical sensor of surface plasmon resonance structure.
With surface plasmon resonance technical construction Optochemical sensor is the research focus of the sensor field nineties.This kind method for sensing can obtain important application aspect the research of interaction of biomacromolecules.Up to now, the prism coupling type surface plasmon resonance sensor of being reported all adopts fixedly incident wavelength, adjusts the measurement pattern that incident angle of light is made variable.Such as by " Biochemistry, Vol.33, No.46,1994,13707 " the disclosed experimental provision that is used for surface plasmon resonance research, promptly be an example.The section of structure of this device as shown in Figure 1.Light source 1 adopts the He/Ne laser instrument, is the light source of single wavelength therefore, incides angle on the prism 3 in order to adjust incident ray 2, needs most of member of whole device is installed on the universal stage 4, and reflection ray 5 is received by detector 6.Material to be analyzed is housed in the sample chamber 7,, produces total reflection, form evanescent wave but light wave still has part to enter into solution at the interface of high reflecting metal film 10 and sample chamber 7 solution when incident ray 2 at an angle during incident.Change incident angle, resonate up to evanescent wave and surface plasma excimer, this moment, intensity of reflected light sharply descended, because surface plasmon resonance is relevant with the specific inductive capacity of solution, and the latter is relevant with the composition of sample solution, so, can carry out quantitative test to sample by measuring the relation of reflective light intensity and incident angle.In the one type of prior art syringe, for the incident angle that changes incident ray 2 must be provided with a big universal stage 4, the accurate parts that take measurement of an angle (need be accurate to 0.01 °) must be arranged simultaneously, in addition, must seal with machine oil between prism 3 and the sample chamber 7, so each sensing element (prism) can only disposablely use, it is numerous and diverse to install huge heaviness, cost is very high, uses also inconvenient.
The present invention is the shortcoming that overcomes prior art, design and a kind ofly be fixed into incident angle of the entering ray and make variable with the incident ray wavelength, the device of detection of reflected light intensity and wavelength relationship is finished the qualitative and quantitative analysis to sample, it is easy to reach device, the purpose that be easy to test, cost is low.
All wavelength surface plasma excimer resonance photochemical sensor of the present invention, adopt the light source of continuous wavelength and use the fiber optic transmission mode, and data are handled with computing machine, thereby no longer need each member one prism, flow cell, detecting device etc. are placed on the universal stage of a heaviness, also needn't be again with the high parts that take measurement of an angle of precision, thus realize purpose of the present invention.
Concrete structure of the present invention is provided by Fig. 2.Wherein still include light source 1, prism 3, detecting device 6 etc.The bottom surface of prism 3 is coated with the metallic film 10 of high reflection, and the incident ray 2 that light source 1 sends is through prism 3 reflections, and reflection ray 5 is received by detecting device 6, and reflective light intensity is detected.The present invention is different from prior art constructions to be had, and light source 1 is the continuous wavelength light source; Behind the incident ray 2 process parallel polarization light pipes 12, incide in the prism 3 with parallel rays; The structure of flow cell 11 and effect are similar to sample chamber of the prior art 7, between it and the prism 3 by washer sealing, flow cell 11 has sample introduction capillary exit 14 and waste liquid capillary exit 13, and the sample to be analyzed in the sample cell 16 pumps into flow cell 11 by peristaltic pump 15 and sample introduction capillary exit 14; Reflection ray 5 focuses on an end of light transmitting fiber 18 through convergent lens 17, transfers to grating 19 and detecting device 6, and the data that detecting device 6 detects machine 20 are as calculated handled and obtained the spectrogram of intensity of reflected light with wavelength variations.
Light source 1 can be used the continuous light source lamp, wavelength coverage 200～800nm, and place on the multidimensional adjusting bracket, can adorn the disk of a band scale on the frame face, be used to show the angle of incident ray 2, the degree of accuracy of scale is less demanding.Said parallel polarization light pipe 12 is tubes of the both ends open printing opacity of interior dress polaroid 21 and two lens 22; Flow cell 11 can be contained on the multidimensional adjusting bracket, so that regulate light path, convergent lens 17 also can be contained on the multidimensional adjusting bracket, to regulate the reflection ray 5 that penetrates through prism 3, makes its coupling focus on an end of light transmitting fiber 18.The multidimensional adjusting bracket does not draw among Fig. 2.
For prism 3 can repeatedly be used, change machine oil encapsulating method of the prior art, and adopt washer sealing.Specific practice is, install rubber washer additional in go to the bottom reflecting surface and the contact position above the flow cell 11 of prism 3, on prism 3, respectively put a poly (methyl methacrylate) plate with flow cell 11 bottom surfaces, again two poly (methyl methacrylate) plates are connected and fixed clamping with adjusting screw(rod), make the prism 3 that is clipped in wherein closely contact sealing with 11 of flow cells.
Embodiment:
The thick silverskin of one deck 50nm is plated as high reflecting metal film 10 in prism 3 surfaces; With Fullwave software and mercury lamp, sodium vapor lamp calibration light source wavelength; Detecting device 6 adopts the ccd array detecting device.Flow cell 11 with prism 3 sealing back horizontal positioned, is at first inserted distilled water by packing ring in flow cell 11; The incident ray 2 that adjusting multidimensional adjusting bracket sends light source 1 is about 10 ° with the horizontal direction angle.Can select incident ray 2 and horizontal direction angle to debug between 0 °～20 ° angles during use, until resonance effect occurring and honeybee shape is best, fixed light source 1 is opened peristaltic pump 15 sample introductions, and computing machine 20 record intensities of reflected light are with the spectrogram of wavelength variations.With this measurement sensitivity of installing ethanol is 4.6 * 10
-4Refractive index unit (575.0nm), with the method for inspection contrast of national Specification, relative error is between+1.5%～-0.37%.
All wavelength surface plasma excimer resonance photochemical sensor of the present invention with prior art relatively have with Lower several remarkable advantages.
(1) because light source is the light source of continuous wavelength, detector is the ccd array detector, so the thing that can study The matter expanded range has the material of resonance response all can study in 200～800nm wave-length coverage.
(2) owing to take resonant wavelength as measured value, so need not adjust incident angle of light in the experimentation, only need Fix an incident angle according to the characteristic of material to be studied before experiment, all parts are not in the experimentation Need to resemble and rotate the prior art, thus can dispense a turntable, thereby cost is relatively low, and operation Easier.
(3) owing to do not do variable with incident angle of light, so do not need accurate angular surveying parts, also make into This reduction.
(4) after prism uses up and takes off, can also reinstall repeatedly and to use, and in the prior art sample room with With the machine oil sealing, each sensing element prism can only disposablely use between the sensing element prism.
(5) experimental implementation is simple, opens peristaltic pump, and sample circulates after the pond, can show resonance figure and resonance wave long value on the computer screen, need not do other and adjust, and prior art needs constantly to adjust the incident angle of incident ray.
(6) good stability, response is rapid, and the life-span is longer, measures accurately.
Description of drawings:
Fig. 1 is the one type of prior art syringe structure diagram.
Fig. 2 is apparatus of the present invention structure diagrams.
Claims (3)
1, a kind of all wavelength surface plasma excimer resonance photochemical sensor, its structure includes light source (1), prism (3), detecting device parts such as (6), the bottom surface of prism (3) is coated with high reflective metal film (10), the incident ray (2) that light source (1) sends reflects through prism (3), reflection ray (5) is received and is detected by detecting device (6), it is characterized in that light source (1) is the continuous wavelength light source; Incident ray (2) incides in the prism (3) with parallel rays behind parallel polarization light pipe (12); By washer sealing, flow cell (11) has sample introduction capillary exit (14) and waste liquid capillary exit (13) above the flow cell (11) and between prism (3) bottom surface; Sample to be analyzed in the sample cell (16) pumps into flow cell (11) by peristaltic pump (15) and sample introduction capillary exit (14); Reflection ray (5) focuses on an end of light transmitting fiber (18) through convergent lens (17), transfers to grating (19) and detecting device (6); The data that detecting device (6) detects machine (20) are as calculated handled, and obtain the spectrogram of intensity of reflected light with wavelength variations.
According to the described all wavelength surface plasma excimer resonance photochemical sensor of claim 1, it is characterized in that 2, light source (1) is a continuous light source, and place on the multidimensional adjusting bracket; Said parallel polarization light pipe (12) is the tube of the both ends open printing opacity of interior dress polaroid (21) and two lens (22); Flow cell (11) is contained on the multidimensional adjusting bracket; Convergent lens (17) is contained on the multidimensional adjusting bracket.
3, according to claim 1 or 2 described all wavelength surface plasma excimer resonance photochemical sensors, it is characterized in that, washer sealing between said flow cell (11) and the prism (3), be prism (3) go to the bottom reflecting surface and above the flow cell (11) contact position install rubber washer additional, on prism (3) and flow cell (11) bottom surface respectively put a poly (methyl methacrylate) plate, be connected and fixed clamping with adjusting screw(rod) again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98115735 CN1095543C (en) | 1998-07-01 | 1998-07-01 | All wavelength surface plasma excimer resonance photochemical sensor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98115735 CN1095543C (en) | 1998-07-01 | 1998-07-01 | All wavelength surface plasma excimer resonance photochemical sensor |
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| Publication Number | Publication Date |
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| CN1203382A CN1203382A (en) | 1998-12-30 |
| CN1095543C true CN1095543C (en) | 2002-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 98115735 Expired - Fee Related CN1095543C (en) | 1998-07-01 | 1998-07-01 | All wavelength surface plasma excimer resonance photochemical sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100359318C (en) * | 2006-02-15 | 2008-01-02 | 厦门大学 | Ultraviolet-ray visible absorbing/fluorescent dual-purpose flow cell |
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| CN100343654C (en) * | 2004-11-30 | 2007-10-17 | 国家海洋环境监测中心 | Surface plasma resonance detection sample cell with repeatable response |
| CN100567955C (en) * | 2005-07-21 | 2009-12-09 | 中国科学院光电技术研究所 | A kind of biochemical molecular detecting method |
| CN100575927C (en) * | 2006-05-18 | 2009-12-30 | 中国科学院化学研究所 | Resonance imager of light-exciting color surface piasma |
| CN100545631C (en) * | 2006-05-18 | 2009-09-30 | 中国科学院化学研究所 | Multifunctional light absorption, scattering and emission spectrometer based on surface plasma-wave |
| CN101398378B (en) * | 2008-01-28 | 2011-06-15 | 国家纳米科学中心 | Phase measurement method of surface plasma resonance and measuring system thereof |
| CN101451952B (en) * | 2008-12-01 | 2011-01-12 | 华东师范大学 | Method implementing wideband optical spectrum surface plasma resonance |
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| CN105548093A (en) * | 2016-01-23 | 2016-05-04 | 宜春学院 | High-sensitivity oscillating field optical waveguide sensor |
| CN109444078A (en) * | 2019-01-14 | 2019-03-08 | 兰州理工大学 | Length scanning index sensor based on unsymmetrical metal cladding Medium Wave Guide |
| CN113406017A (en) * | 2021-06-17 | 2021-09-17 | 桂林电子科技大学 | High-integration surface plasma resonance sensor system |
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1998
- 1998-07-01 CN CN 98115735 patent/CN1095543C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100359318C (en) * | 2006-02-15 | 2008-01-02 | 厦门大学 | Ultraviolet-ray visible absorbing/fluorescent dual-purpose flow cell |
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| CN1203382A (en) | 1998-12-30 |
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