CN109536366A - 一种基于改性毛细管的核酸检测微流控芯片及核酸检测*** - Google Patents
一种基于改性毛细管的核酸检测微流控芯片及核酸检测*** Download PDFInfo
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- CN109536366A CN109536366A CN201811443896.5A CN201811443896A CN109536366A CN 109536366 A CN109536366 A CN 109536366A CN 201811443896 A CN201811443896 A CN 201811443896A CN 109536366 A CN109536366 A CN 109536366A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B—PERFORMING OPERATIONS; TRANSPORTING
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Cited By (23)
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---|---|---|---|---|
CN109991346A (zh) * | 2019-04-18 | 2019-07-09 | 南京大学 | 一种用于有机氮分析的微流控紫外氧化装置 |
CN110257240A (zh) * | 2019-07-16 | 2019-09-20 | 东莞博识生物科技有限公司 | 核酸提取、扩增及检测一体试剂卡 |
CN110452803A (zh) * | 2019-08-27 | 2019-11-15 | 东南大学 | 一种核酸快速扩增检测方法及装置 |
CN111004719A (zh) * | 2019-12-03 | 2020-04-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | 核酸检测模块、检测单元及检测*** |
CN111073811A (zh) * | 2019-12-24 | 2020-04-28 | 深圳市华迈生物医疗科技有限公司 | 一种用于实时荧光核酸扩增检测的微流控芯片及检测方法 |
CN111197003A (zh) * | 2019-12-26 | 2020-05-26 | 中国科学院合肥物质科学研究院 | 一种基于智能手机的集成核酸提取扩增检测的分析装置及方法 |
CN111378573A (zh) * | 2020-03-20 | 2020-07-07 | 中国科学院长春光学精密机械与物理研究所 | 微管道核酸扩增*** |
CN111575152A (zh) * | 2020-05-27 | 2020-08-25 | 合肥中科易康达生物医学有限公司 | 一种集成核酸提取扩增检测功能于一体的封闭式卡盒及检测方法 |
CN111760601A (zh) * | 2020-07-03 | 2020-10-13 | 中国科学院合肥物质科学研究院 | 一种集成液路切换阀的微流控芯片及核酸检测方法 |
CN111948409A (zh) * | 2019-05-15 | 2020-11-17 | 深圳市理邦精密仪器股份有限公司 | 一种微流控测试卡、测试卡组件、设备及控制方法 |
CN113150979A (zh) * | 2020-12-30 | 2021-07-23 | 北京航空航天大学 | 基于微孔阵列芯片的便携式核酸检测仪 |
CN113234571A (zh) * | 2021-04-29 | 2021-08-10 | 杭州霆科生物科技有限公司 | 一种高通量酶筛选芯片 |
CN113244970A (zh) * | 2021-01-07 | 2021-08-13 | 中国检验检疫科学研究院 | 一种用于提取核酸和pcr检测的微流控芯片及其应用 |
CN113444624A (zh) * | 2021-06-21 | 2021-09-28 | 清华大学深圳国际研究生院 | 一种依靠拉伸力驱动的核酸检测芯片及核酸检测设备 |
WO2021212276A1 (zh) * | 2020-04-20 | 2021-10-28 | 深圳华大智造科技股份有限公司 | 铺液装置、铺液方法、铺液***及组合装置与过液装置 |
CN113736643A (zh) * | 2021-09-23 | 2021-12-03 | 吉特吉生物技术(苏州)有限公司 | 一种核酸扩增检测微流控芯片 |
CN113832022A (zh) * | 2021-09-23 | 2021-12-24 | 吉特吉生物技术(苏州)有限公司 | 一种一体式核酸扩增检测设备 |
WO2022061521A1 (zh) * | 2020-09-22 | 2022-03-31 | 京东方科技集团股份有限公司 | 核酸提取微流控芯片、核酸提取装置及提取方法 |
CN115155677A (zh) * | 2022-05-23 | 2022-10-11 | 上海仕欧炆基因科技有限公司 | 一种用于多种呼吸道病原体核酸检测的微流控***及其检测方法 |
WO2023020220A1 (zh) * | 2021-08-17 | 2023-02-23 | 京东方科技集团股份有限公司 | 微流控芯片、其核酸提取方法及核酸提取装置 |
CN115747047A (zh) * | 2022-12-29 | 2023-03-07 | 天津大学 | 一种基于特高频声流体核酸光电检测设备 |
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CN117607420A (zh) * | 2023-12-08 | 2024-02-27 | 翊新诊断技术(苏州)有限公司 | 一种免疫分析*** |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060110725A1 (en) * | 2004-11-25 | 2006-05-25 | Jeong-Gun Lee | Apparatus for and method of purifying nucleic acids by different laser absorption of beads |
WO2009108260A2 (en) * | 2008-01-22 | 2009-09-03 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
CN104001562A (zh) * | 2014-06-09 | 2014-08-27 | 南京大学 | 一种用于铬形态快速分离的圆盘离心式微流控芯片及装置 |
CN104549590A (zh) * | 2015-01-26 | 2015-04-29 | 北京工业大学 | 一种金属微流控芯片微通道内壁改性工艺 |
CN105349401A (zh) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | 一种多功能的集成化微流控核酸分析芯片及制备和分析方法 |
CN107649190A (zh) * | 2016-07-26 | 2018-02-02 | 宋岳 | 被测样品液驱动用构件易于卸除的艾滋病诊断用装置 |
-
2018
- 2018-11-29 CN CN201811443896.5A patent/CN109536366B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060110725A1 (en) * | 2004-11-25 | 2006-05-25 | Jeong-Gun Lee | Apparatus for and method of purifying nucleic acids by different laser absorption of beads |
WO2009108260A2 (en) * | 2008-01-22 | 2009-09-03 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
CN104001562A (zh) * | 2014-06-09 | 2014-08-27 | 南京大学 | 一种用于铬形态快速分离的圆盘离心式微流控芯片及装置 |
CN104549590A (zh) * | 2015-01-26 | 2015-04-29 | 北京工业大学 | 一种金属微流控芯片微通道内壁改性工艺 |
CN105349401A (zh) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | 一种多功能的集成化微流控核酸分析芯片及制备和分析方法 |
CN107649190A (zh) * | 2016-07-26 | 2018-02-02 | 宋岳 | 被测样品液驱动用构件易于卸除的艾滋病诊断用装置 |
Non-Patent Citations (2)
Title |
---|
JUNHOU HUI等: "《Multiplex sample-to-answer detection of bacteria using a pipette-actuated capillary array comb with integrated DNA extraction, isothermal amplification, and smartphone detection》", 《LAB ON A CHIP》 * |
WEIHUA HU等: "《A portable flow-through fluorescent immunoassay lab-on-a-chip device using ZnO nanorod-decorated glass capillaries》", 《LAB ON A CHIP》 * |
Cited By (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109991346A (zh) * | 2019-04-18 | 2019-07-09 | 南京大学 | 一种用于有机氮分析的微流控紫外氧化装置 |
CN111948409A (zh) * | 2019-05-15 | 2020-11-17 | 深圳市理邦精密仪器股份有限公司 | 一种微流控测试卡、测试卡组件、设备及控制方法 |
CN111948409B (zh) * | 2019-05-15 | 2024-05-14 | 深圳市理邦精密仪器股份有限公司 | 一种微流控测试卡、测试卡组件、设备及控制方法 |
CN110257240B (zh) * | 2019-07-16 | 2024-04-09 | 深圳博识诊断技术有限公司 | 核酸提取、扩增及检测一体试剂卡 |
CN110257240A (zh) * | 2019-07-16 | 2019-09-20 | 东莞博识生物科技有限公司 | 核酸提取、扩增及检测一体试剂卡 |
CN110452803A (zh) * | 2019-08-27 | 2019-11-15 | 东南大学 | 一种核酸快速扩增检测方法及装置 |
CN111004719A (zh) * | 2019-12-03 | 2020-04-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | 核酸检测模块、检测单元及检测*** |
CN111073811A (zh) * | 2019-12-24 | 2020-04-28 | 深圳市华迈生物医疗科技有限公司 | 一种用于实时荧光核酸扩增检测的微流控芯片及检测方法 |
CN111197003A (zh) * | 2019-12-26 | 2020-05-26 | 中国科学院合肥物质科学研究院 | 一种基于智能手机的集成核酸提取扩增检测的分析装置及方法 |
CN111197003B (zh) * | 2019-12-26 | 2023-08-25 | 中国科学院合肥物质科学研究院 | 一种基于智能手机的集成核酸提取扩增检测的分析装置及方法 |
CN111378573A (zh) * | 2020-03-20 | 2020-07-07 | 中国科学院长春光学精密机械与物理研究所 | 微管道核酸扩增*** |
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