CN109536296B

CN109536296B - Antibacterial biological detergent for infants and preparation method thereof

Info

Publication number: CN109536296B
Application number: CN201811641083.7A
Authority: CN
Inventors: 陈欣欣
Original assignee: Guangdong Jumei Daily Necessities Co Ltd
Current assignee: Guangdong Jumei daily necessities Co., Ltd
Priority date: 2018-12-29
Filing date: 2018-12-29
Publication date: 2021-02-02
Anticipated expiration: 2038-12-29
Also published as: CN109536296A

Abstract

The invention discloses an infant antibacterial biological detergent which is prepared from the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water. The infant antibacterial biological detergent disclosed by the invention is simple to prepare, has no toxicity or extremely low toxicity on the used components, has no stimulation to skin, has low residue, does not threaten the health of infants, is rich and fine in foam, strong in detergency, strong in hard water resistance and good in solubility, and has good killing and inhibiting effects on various bacteria and moulds.

Description

Antibacterial biological detergent for infants and preparation method thereof

Technical Field

The invention relates to the technical field of microorganisms, in particular to an infant antibacterial biological detergent and a preparation method thereof.

Background

Detergents and disinfectants are indispensable commodities in human social life and bear important duties of keeping environment clean and sanitary and ensuring human health and safety. With the gradual increase of the health consciousness of people, new requirements on detergents and disinfectants are also put forward. At present, the detergent and the disinfectant mainly have the problems that the dual requirements of washing and disinfection cannot be met simultaneously, the sterilizing effect of synthetic and plant-source bactericides is poor, unreasonable use causes harm to human health and the like.

The liquid detergent has the advantages of good solubility, no dust generation, less residue, convenient use and the like, and the demand in production, life and use is continuously increased. In order to meet the performance requirements of detergency, solubility, easy rinsing and the like, the existing liquid detergent is added with too many synthetic compounds, and some of the compounds are difficult to degrade and some of the compounds have toxic effects and are harmful to the environment and human health. The use of large amounts of synthetic surfactants in existing detergents has led to the annual expansion of contaminated waters. Although the surfactants with poor biodegradability are basically eliminated at present, the widely used surfactants are biodegradable. However, even if it isSome surfactants with low concentration can also generate a large amount of foam, and the large amount of foam which is not easy to disappear forms an isolation layer on the water surface, so that the gas exchange between the water body and the atmosphere is weakened, the water body smells, the aesthetic feeling of the natural water body landscape is influenced, and the biotoxicity of the surfactant also directly threatens the survival of aquatic animals and plants. The waste water containing the detergent is discharged into the water environment in large quantity, and the components thereof can be biodegraded to cause the exhaustion of dissolved oxygen and mineral substances in the water, so that the dissolved oxygen in the water is obviously reduced, and the concentration exceeds 8 mg.L^-1It also can poison aquatic organisms and fish.

Most of stains, sweat, milk, oil and the like of baby products are protein, and most of the existing detergents adopt a mode of directly adding protease, and the protease is easy to inactivate or react with other detergents due to environmental pollution in the processes of storage, transportation and use, so that the stain removal effect is difficult to exert.

The detergent added with live probiotics and biological enzyme develops rapidly in recent years and obtains better effect, but the safety problem of the live probiotics also arouses attention of people, the risk of increasing pathogenic bacteria exists in the mutation and transfer of thalli, and the risk is higher especially for patients with low immunity. In addition, the proper conditions required by the activity of the live bacteria and the biological enzyme greatly limit the production, transportation and use range, and the cost is correspondingly improved.

Most of infant laundry detergents in the market at present mainly emphasize the decontamination function of detergent and neglects mildness and nontoxicity, and in addition, because the mouth is a tool for infants to explore the world, such as a piece of saliva towel, clothes, toys and the like, the infants are licked by mouth, and the residual irritative and toxic components on the infant laundry detergents after being washed pose threats to the health of the infants.

Disclosure of Invention

Aiming at the defects of the existing detergent, the invention provides the infant antibacterial biological detergent which has the advantages of sterilization and odor removal, good decontamination effect, easy biodegradation, no toxic or side effect and low cost.

The purpose of the invention is realized by the following technical scheme:

an antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water.

Further, the preparation method of the tea saponin aqueous extraction fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth.

Further, the preparation method of the soapberry aqueous extract fermentation liquor comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain the soapberry water extract fermentation liquor.

Further, the glycolipid biosurfactant is one or more of rhamnolipid, sophorolipid, trehalose glycolipid, cellobiolipid and mannosylerythritol lipid.

Further, the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio of the inactivated probiotics to the bifidobacteria is 2:2: 1.

Further, theThe preparation method of the microcapsule for encapsulating protease comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl₂And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.

A preparation method of an infant antibacterial biological detergent comprises the following steps:

step one, taking raw materials according to the weight part ratio;

dissolving the nano degraded chitosan in deionized water, and stirring until the nano degraded chitosan is dissolved to obtain a nano degraded chitosan solution;

and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH value, and continuously uniformly stirring to obtain the product.

The tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor used in the invention do not relate to an organic solvent in the whole preparation process, the fermentation liquor contains natural nonionic surfactant tea saponin and soapberry saponin, the toxicity and the irritation to the skin are very low, the surface tension of water can be reduced, the foam is rich and fine, the detergency is strong, the hard water resistance is strong, the dissolubility is good, and the degreasing property is moderate. Can also remove metal poisons on the surface of the human body, and has a high elution rate of lead, mercury, manganese, arsenic, chromium and other heavy metals up to 90 percent.

The glycolipid biosurfactant used in the invention can obviously reduce surface tension and has multiple functions of decontamination, emulsification, washing, dispersion, wetting, permeation, diffusion, foaming, antioxidation, viscosity regulation, sterilization, antistatic property and the like. The glycolipid biosurfactant has good thermal and chemical stability, and in addition, the trehalose glycolipid is resistant to strong acid and strong alkali, and the rhamnolipid has strong anti-mildew activity. The coco glucoside has excellent detergency, foaming, foam stabilizing, emulsifying, dispersing, solubilizing, wetting and penetrating abilities, acid resistance, alkali resistance and insensitivity to electrolyte. Can reduce irritation of other surfactants, and has good compatibility with skin and lower irritation.

The nano degradable chitosan used in the invention has the particle size of 100-500 nm, has good solubility in water, can rapidly enter the interior of the fabric, and is combined with the fabric through the protonated amino group to form a self-assembled film on the surface of the fabric, so that the fabric can be prevented from hardening, the skin-friendly property of the fabric is improved, and the nano degradable chitosan can play a role in moisturizing and skin care when contacting with the skin. The polyaspartic acid used in the invention can play a role in softening water, can cooperate with chitosan to soften fabrics, has certain moisturizing, sterilizing and softening effects on skin, has low irritation and toxicity, and has good biocompatibility with eyes and skin.

The encapsulated protease microcapsule is prepared by adopting chitosan-sodium alginate as a wall material and alkaline protease as a core material through a one-step emulsification method, has a controllable structure and good balling property, has an embedding rate as high as 65 percent, achieves a good encapsulating effect and a release function, and solves the problem that the activity of the alkaline protease is reduced due to interference of other washing components and influence of a washing environment in the storage and application processes of a detergent.

The tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor used in the invention greatly improve the purity of excellent natural nonionic surfactants tea saponin and soapberry saponin in the fermentation liquor through fermentation, the two surfactants have strong decontamination capability, stable property, good foamability and long foam fading time, have good removal effect on hydrophobic organic compounds, sebum, oil stain and the like, have low cost, safety and rapid fermentation and decomposition, do not cause any pollution to the environment, and do not bring negative problems of fluorescent agent, surfactant, environmental hormones, river eutrophication and the like. In addition, the tea saponin water extraction fermentation liquor contains tea saponin, various active ingredients with strong inhibition effect on escherichia coli, staphylococcus aureus and aspergillus niger, and the soapberry water extraction fermentation liquor contains a large amount of rhamnolipid besides soapberry saponin, and the rhamnolipid and the soapberry saponin are cooperated to enhance the decontamination effect.

The inactivated probiotics is added into the infant detergent, and in the washing process, the probiotic cells can be combined with the fabric and the chitosan self-assembly layer on the surface of the fabric and can be adhered to the surface of the skin when contacting the skin, so that other harmful bacteria, heavy metals and the like are prevented from being combined with the skin, and the incidence rate of infant eczema and dermatitis is reduced.

Compared with the prior art, the invention has the following beneficial effects:

(1) the components of the infant antibacterial biological detergent provided by the invention are nontoxic or extremely low in toxicity, have no stimulation to skin, are safe to use, have low residue, do not threaten the health of infants, and have light tea oil fragrance, the used nano degraded chitosan and polyaspartic acid can soften and make fabrics skin-friendly, and when the nano degraded chitosan and polyaspartic acid are used as a clothes detergent, the nano degraded chitosan and inactivated probiotics can prevent clothes of infants from hardening and rubbing the skin after being washed, and can protect the skin, so that the incidence rate of eczema and dermatitis of infants is reduced;

(2) the infant antibacterial biological detergent provided by the invention can reduce the surface tension of water, has rich and fine foam, strong detergency, strong hard water resistance and good solubility, has good killing and inhibiting effects on various bacteria and moulds, can ensure the activity of protease in the storage and application processes of the detergent, has a good removing effect on most common milk stains, sweat stains and the like on infant products, can be applied to cleaning of infant clothes, and can also be applied to cleaning of infant tableware, toys and other products;

(3) according to the infant antibacterial biological detergent provided by the invention, the tea saponin water-extraction fermentation liquor and the soapberry water-extraction fermentation liquor have high purity of the tea saponin and the soapberry saponin, and also contain the glycolipid surfactant and the antibacterial active substance, so that the detergent has good detergency and antibacterial activity, is low in cost and safe, can be rapidly fermented and decomposed, and cannot cause any pollution to the environment;

(4) the preparation method of the infant antibacterial biological detergent is simple to operate, clearly limits the feeding sequence, ensures the uniformity and stability of the detergent, and avoids the layering phenomenon.

Detailed Description

To further illustrate the technical measures taken by the present invention and the effects thereof, the following detailed description is given with reference to preferred embodiments of the present invention.

Example 1

An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 2 parts of tea saponin water extraction fermentation liquor, 3 parts of soapberry water extraction fermentation liquor, 5 parts of glycolipid biosurfactant, 5 parts of nano degraded chitosan, 0.05 part of polyaspartic acid, 0.57 part of arginine, 0.3 part of coco glucoside, 5 parts of encapsulated protease microcapsule, 2 parts of inactivated probiotics and 45 parts of deionized water.

Further, the glycolipid biosurfactant is rhamnolipid or sophorolipid.

Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl₂And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.

step one, taking raw materials according to the weight part ratio;

Example 2

An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 5 parts of tea saponin water extraction fermentation liquor, 6 parts of soapberry water extraction fermentation liquor, 8 parts of glycolipid biosurfactant, 8 parts of nano degraded chitosan, 0.08 part of polyaspartic acid, 1.68 parts of arginine, 2.5 parts of coco glucoside, 8 parts of encapsulated protease microcapsule, 3 parts of inactivated probiotics and 52 parts of deionized water.

Further, the glycolipid biosurfactant is a trehalose glycolipid.

Further, the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying sodium alginate solution in liquid paraffin containing span80Stirring at a warm temperature to form emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl₂And (3) dropping the mixture into the sodium alginate emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after the dropping is finished, demulsifying and layering the mixture by using isopropanol, filtering, and washing the mixture for multiple times by using distilled water to obtain the microcapsule.

step one, taking raw materials according to the weight part ratio;

Example 3

An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 5 parts of tea saponin water extraction fermentation liquor, 6 parts of soapberry water extraction fermentation liquor, 8 parts of glycolipid biosurfactant, 8 parts of nano degraded chitosan, 0.08 part of polyaspartic acid, 2.49 parts of arginine, 3.4 parts of coco glucoside, 8 parts of encapsulated protease microcapsule, 3 parts of inactivated probiotics and 52 parts of deionized water.

Further, the glycolipid biosurfactant is a trehalose glycolipid.

step one, taking raw materials according to the weight part ratio;

Example 4

An antibacterial biological detergent for infants is prepared from the following raw materials in parts by weight: 7 parts of tea saponin water extraction fermentation liquor, 8 parts of soapberry water extraction fermentation liquor, 10 parts of glycolipid biosurfactant, 10 parts of nano degraded chitosan, 0.1 part of polyaspartic acid, 3.02 parts of arginine, 7 parts of coco glucoside, 10 parts of encapsulated protease microcapsule, 4 parts of inactivated probiotics and 65 parts of deionized water.

Further, the glycolipid biosurfactant is rhamnolipid, cellobiolipid and mannosylerythritol lipid.

step one, taking raw materials according to the weight part ratio;

Stability test

The detergents prepared in examples 1 to 4 were subjected to stability tests according to national standard GB9985-200, and the experimental results are shown in Table 1.

TABLE 1 stability test results

Temperature of	Example 1	Example 2	Example 3	Example 4
					-5℃	Without delamination	Without delamination	Without delamination	Without delamination
25℃	Without delamination	Without delamination	Without delamination	Without delamination
					40℃	Without delamination	Without delamination	Without delamination	Without delamination

As can be seen from Table 1, the antibacterial biological detergents for infants prepared in examples 1 to 4 have no delamination at-5 ℃, 25 ℃ and 40 ℃, which indicates that the detergents provided by the invention have stable states at various temperatures.

Routine index detection

The results of the tests conducted with reference to the national standards for the requirements of hand dishwashing detergents are shown in Table 2.

TABLE 2 results of conventional index detection

As can be seen from Table 2, the infant antibacterial bio-detergent prepared in the examples 1-4 does not contain fluorescent agent, methanol, formaldehyde and other harmful substances, and meets the national standard and middle index requirements of tableware detergent.

Comparative example 1

The procedure is as described in example 3, except that the glycolipid biosurfactant is not included.

Comparative example 2

The procedure is as in example 3 except that polyaspartic acid is not contained.

Comparative example 3

The procedure is as in example 3 except that the probiotic is not inactivated.

Performance testing

The following performance tests were performed on the detergents obtained in examples 1 to 4 and comparative examples 1 to 3, respectively, and the test methods and results were as follows:

1 foam Property measurement

The measurement was carried out according to the method for measuring foaming power of detergent in GB/T13173-2008 (Ross-Mile method). Hard water formulations are described in GB/T6367-1997. The foaming ability and foam stability of the test specimens were determined by measuring the foam height (mm) at 0, 5 and 10 min. The test conditions were w (detergent) 0.2% in aqueous solution, water hardness 250 and 150mg/L, respectively, and temperature (40 ± 0.5) ° c. The measurement results are shown in Table 3.

TABLE 3 foam Performance test results

As can be seen from Table 3, the water hardness is 250 and 150mg/L respectively, the temperature is (40 +/-0.5) DEG C, the foam height of the examples 1-4 is greater than 110mm at 0min, and the foam height is still more than 90mm at 10min, which shows that the detergent provided by the invention has good foamability, the foam fading is slow, and the foam performance is hardly influenced by the change of the water hardness. Comparative example 1 contains no glycolipid biosurfactant, and compared with example 3, the foam height is greatly reduced and the foam fading is faster, compared with example 3, which shows that the glycolipid biosurfactant has synergistic effect with tea saponin water extraction fermentation liquor and soapberry water extraction fermentation liquor in terms of foamability and foam fading rate. Comparative example 2 contains no poiyamenine, and compared to example 3, the foam height and fade were not significantly different from example 3, indicating that poiyamenine had no effect on the foam of the system. Comparative example 3 contained no inactivated probiotic bacteria, and compared to example 3, the foam height was slightly reduced and the foam regressed considerably, indicating that the inactivated probiotic bacteria had some effect on the system foam system.

2 detergency test

The determination is carried out according to the determination method of the detergency and the cycle washing performance of the washing agent for the clothing in GB/T13174-2008. Hard water formulations are described in GB/T6367-1997. The detergency of the samples was determined by measuring the washing conditions of the samples with respect to carbon black soiled cloth, protein soiled cloth and sebum soiled cloth. The test conditions were w (detergent) 0.2% in aqueous solution, water hardness 250 and 150mg/L, respectively, and temperature (40 ± 0.5) ° c. The measurement results are shown in Table 4.

TABLE 4 detergency test results

As can be seen from Table 4, the detergents obtained in examples 1 to 3 have good detergency on carbon black soiled cloth, protein soiled cloth and sebum soiled cloth, and the hardness of water has no influence on the detergency. Comparative example 1 does not contain glycolipid biosurfactant, and the detergency of the detergent is reduced compared with that of example 3, which shows that the glycolipid biosurfactant has a synergistic effect with tea saponin water extraction fermentation liquor and soapberry saponin water extraction fermentation liquor; comparative example 2 contains no polyaspartic acid, and the detergency is slightly reduced, indicating that polyaspartic acid has a promoting effect on the detergency efficacy of the surfactant in addition to the polysuppleness efficacy; comparative example 3, which contained no inactivated probiotic bacteria, had a slight decrease in detergency, probably due to the presence of detersive active ingredients in the metabolites contained in the live probiotic bacteria.

3 bacteriostatic test

According to QB/T2738-. Respectively adopting stock solution and solution with the mass fraction of 1%, acting for 20min, respectively determining the bacteriostatic rate of the detergent on staphylococcus aureus and escherichia coli by a suspension quantitative method, repeating the experiment for 3 times, and taking the average value as the final result, wherein the result is shown in table 5.

TABLE 5 results of the bacteriostatic test

As can be seen from Table 5, the detergents obtained in examples 1 to 3 have good bacteriostatic effects on Staphylococcus aureus and Escherichia coli, and have almost no influence on bacteriostatic activity after being diluted by 200 times. Comparative example 1 in addition to the absence of glycolipid biosurfactant, the bacteriostatic activity of the detergent was reduced compared to example 3, indicating that the glycolipid biosurfactant is closely related to the bacteriostatic activity of the detergent; comparative example 2 contains no polyaspartic acid, and the bacteriostatic activity is slightly reduced compared with example 3, which shows that polyaspartic acid plays an important role in the aspect of good bacteriostatic activity of the detergent; comparative example 3 contains no inactivated probiotic bacteria and the bacteriostatic activity is also reduced, probably because the bacteriostatic metabolic products contained in the inactivated probiotic bacteria play a key role in the bacteriostatic activity of the detergent.

In conclusion, the components of the baby antibacterial biological detergent provided by the invention have synergistic effect, the foamability is good, the foam retention time is long, the effect of uniformly and well removing various stains is achieved, the antibacterial biological detergent is strong in antibacterial activity, and the baby antibacterial biological detergent can be directly used as a baby product detergent or applied to baby tableware, toys and clothes detergent products.

The above description is only for the specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and other modifications or equivalent substitutions made by the technical solution of the present invention by the ordinary skilled in the art should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. An antibacterial biological detergent for infants is characterized by comprising the following raw materials in parts by weight: 2-7 parts of tea saponin water extraction fermentation liquor, 3-8 parts of soapberry water extraction fermentation liquor, 5-10 parts of glycolipid biosurfactant, 5-10 parts of nano degraded chitosan, 0.05-0.1 part of polyaspartic acid, 0.2-5 parts of arginine, 0.3-7 parts of coco glucoside, 5-10 parts of encapsulated protease microcapsule, 2-4 parts of inactivated probiotics and 45-65 parts of deionized water;

the glycolipid biosurfactant is one or more of rhamnolipid, sophorolipid, trehalose glycolipid, cellobiose and mannose erythritol lipid; the particle size of the nano degradable chitosan is 100-500 nm; the inactivated probiotics are heat inactivated lactobacillus casei, lactobacillus paracasei and bifidobacteria, and the weight part ratio is 2:2: 1;

the preparation method of the tea saponin aqueous extract fermentation liquor comprises the following steps: (1) crushing the oil-tea camellia cake into particles, and sieving the particles with a 80-mesh sieve for later use; (2) placing the sieved oil-tea camellia cake in an extraction container, adding deionized water at a solid-to-liquid ratio of 8:1, and heating and extracting in a water bath to obtain an extracting solution; (3) centrifuging the obtained extracting solution, adding a 1% alum solution, stirring uniformly, standing for 1.5-2 h, centrifuging, taking supernatant, and concentrating the supernatant under reduced pressure to obtain tea saponin water extract; (4) inoculating lactobacillus into tea saponin water extractive solution according to 1.5% inoculation amount, sealing, standing at 30 deg.C for fermentation for 4 days, removing thallus, and decolorizing with powdered activated carbon at 25 deg.C in 120r/min shaking table to obtain tea saponin water extractive fermentation broth;

the preparation method of the soapberry water extraction fermentation liquid comprises the following steps: s1, crushing the dried soapberry peel into particles, and sieving the particles with a 60-mesh sieve for later use; s2, mixing the soapberry particles and distilled water uniformly according to the material-liquid ratio of 1:2, extracting for 5h at 40 ℃, continuously extracting for 3 times, combining the filtrates, and concentrating to obtain a soapberry water extract; s3, inoculating lactobacillus into the soapberry water extract according to the inoculation amount of 1.5%, sealing, standing and fermenting at 30 ℃, removing thalli after fermenting for 4 days, and decoloring by adopting powdered activated carbon in a shaking table at 60 ℃ for 200r/min to obtain soapberry water extract fermentation liquor;

the preparation method of the protease-encapsulated microcapsule comprises the following steps: (1) preparing alkaline protease and sodium alginate into solutions by adding distilled water according to a proportion respectively; (2) emulsifying a sodium alginate solution in liquid paraffin containing span80, and stirring at constant temperature to form an emulsion; (3) dissolving chitosan in acetic acid solution, adding CaCl₂And (3) dripping the mixture into the emulsion prepared in the step (2) under stirring, continuing stirring for half an hour after dripping, demulsifying and layering by using isopropanol, performing suction filtration, and washing by using distilled water for multiple times to obtain the microcapsule.

2. A method of preparing an infant antimicrobial biobased detergent according to claim 1, comprising the steps of:

step one, taking raw materials according to the weight part ratio;

and step three, adding the tea saponin water extraction fermentation liquor and the soapberry water extraction fermentation liquor into a reaction kettle, adding the nano degraded chitosan solution, uniformly stirring, adding the polyaspartic acid, the glycolipid biosurfactant and the coco-glucoside, uniformly stirring, finally adding the encapsulated protease microcapsule and the inactivated probiotics, stirring, adding the arginine to adjust the pH, and continuously uniformly stirring to obtain the product.