CN109423506A - The preparation method of radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals - Google Patents

The preparation method of radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals Download PDF

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Publication number
CN109423506A
CN109423506A CN201710745934.1A CN201710745934A CN109423506A CN 109423506 A CN109423506 A CN 109423506A CN 201710745934 A CN201710745934 A CN 201710745934A CN 109423506 A CN109423506 A CN 109423506A
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CN
China
Prior art keywords
radix cynanchi
cynanchi bungei
fruits
pesticide
vegetables
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Pending
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CN201710745934.1A
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Chinese (zh)
Inventor
程志红
叶凯霞
陈洪生
赵庆新
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JIANGSU BOYI BIOLOGICAL TECHNOLOGY Co Ltd
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JIANGSU BOYI BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201710745934.1A priority Critical patent/CN109423506A/en
Publication of CN109423506A publication Critical patent/CN109423506A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/22—Organic compounds
    • C11D7/40—Products in which the composition is not well defined
    • C11D7/44—Vegetable products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/23—Lactobacillus acidophilus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Abstract

The invention discloses a kind of methods of cleaning agent that fruits and vegetables pesticide is prepared with radix cynanchi bungei waste material.Preparation method of the invention: (1) culture medium is prepared, 1500g radix cynanchi bungei root tuber waste material, 500g radix cynanchi bungei straw powder, 10g brown sugar, 500mL water, is mixed, sterilize 15min under 150 DEG C, 1.5 atmospheric pressure；(2) it ferments, successively accesses bacillus pumilus, saccharomyces cerevisiae and lactobacillus acidophilus, be left to ferment 15d at 28 DEG C；(3) it separates, it is radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals that filter centrifugation, which obtains supernatant, and low temperature drying obtains pulvis, 4 DEG C of preservations after packing.Product of the invention is safety micro-organism enzyme preparation, under 10mg/L concentration, the Multiple Pesticides such as degradable 15ppm methyl sulfur bacterium phosphorus and lambda-cyhalothrin in 10min.

Description

The preparation method of radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals
Specification
Technical field
The present invention relates to a kind of fruits and vegetables cleaning agent of degrading pesticide, technical field belongs to microbial engineering field, should Fruits and vegetables cleaning agent produces based on microbial fermentation, and for fermentation raw material based on radix cynanchi bungei waste material, suitable application area is fruits and vegetables cleaning.
Background technique
In the producing, processing and selling of fruits and vegetables and using in link, is sold and lagged due to fruits and vegetables resource seasonality, generate money Source property wasting phenomenon, in addition, there are the utilization demands of fruits and vegetables waste material resource in fruit and vegetable processing industry, it would be highly desirable to utilization technology.
The fermentation technique of fruits and vegetables resource and fruits and vegetables waste material is mainly used fruits and vegetables as base-material, is handled using microbial fermentation, obtained It is mainly the type products such as fermented type vegetables, microbial bacterial agent or fruit wine to product.The base-material of traditional ferment production used Mainly there are raw materials, the bacterial strains used such as corn flour, bean powder, the wooden bran, rice chaff different due to the application purpose of ferment.Base of the invention Material mainly uses radix cynanchi bungei waste material, and the bacterial strain used is fruits and vegetables endophyte and saccharomyces cerevisiae (Saccharomyces Cerevisiae lactobacillus acidophilus (Lactobacillus acidophilus), bacillus pumilus (Bacillus Subtilis Wb800), it is therefore an objective to the specific diversity for most seeking enzyme achievees the purpose that Multiple Pesticides of degrading.
Fruits and vegetables detergent for washing out agricultural chemicals has important application value in food security aspect, has a vast market foreground, by In current fruits and vegetables cultivation, due to generally using pesticide, cause garden stuff pesticide residue phenomenon than more serious, although in the market There is fruits and vegetables detergent for washing out agricultural chemicals, but fruits and vegetables detergent for washing out agricultural chemicals product and existing patent currently on the market, the removal fruits and vegetables residual being related to Pesticide technology has two classes, and composition characteristics are shown, one kind is using molecules surfactant aliphatic ester, conch meal and plant The raw materials such as extract are prepared, second is that the use of microbial cells being main component；Its technical principle feature is that first kind fruits and vegetables pesticide is clear The cleaning function of lotion is mainly by conventional surfactant, and based on the similar technology that mixes, the second class is used based on microbial cells Ingredient is wanted, thallus absorption is relied primarily on.The defect of the first kind is also needed using chemical products, and the defect of the second class is, by bacterium Body directly removes pesticide, and effect is unobvious, and the processing time is long.
Summary of the invention
The purpose of the invention is to provide a kind of enzyme agent of degrading pesticide, feature has three aspects, first is that being its master Wanting ingredient is the compound enzyme system using the micro-organism enzyme preparation with foodsafety, and containing multiple-microorganism generation, energy Degradation Multiple Pesticides, second is that its technical principle is the direct deteriorating pesticide residue of biological enzyme, third is that pure biological agent.
It is another object of the present invention to the preparation methods in order to provide the enzyme agent of this degrading pesticide
The main component of the enzyme agent of degrading pesticide is methyl sulfur bacterium phosphorus degradation enzyme system and lambda-cyhalothrin degradation enzyme system etc. Pesticide degrading enzyme.
The enzyme agent, the work of the pesticides degrading enzyme such as methyl sulfur bacterium phosphorus degradation enzyme system and lambda-cyhalothrin degradation enzyme system Property standard be the remains of pesticide in 1 liter of enzyme agent 10min in degradable 5kg fruits and vegetables sample, the methyl sulfur bacterium phosphorus of fruits and vegetables sample and Lambda-cyhalothrin content is 15ppm, which is that national standard obtains 300 times or so.
The bacillus is bacillus subtilis (the Bacillus subtilis for removing extracellular protease gene WB800), the yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae), and the Lactobacillus lactis is acidophilus cream Bacillus (Lactobacillus acidophilus).
The preparation method of the enzyme agent of the degrading pesticide, specific steps are as follows: by bacillus, yeast and Bacillus acidi lactici It is inoculated into common LB liquid medium, middle culture respectively, logarithmic growth phase mid-term processed is inoculated into fruits and vegetables culture medium, is sent out Ferment, 28 DEG C, ferment 15d, filters, and centrifugation obtains supernatant, as enzyme agent.
The fruits and vegetables culture medium is 200g apple, 200g pear, 200g banana, 200g Chinese cabbage, 200g green vegetables, 200g Huang Melon, 100g grape and 200g wax gourd, 10g brown sugar is unsterilised, retains fruits and vegetables endophyte.
Specific embodiment:
The preparation of the enzyme agent of 1 degrading pesticide
Bacillus subtilis seed culture uses LB culture medium: yeast extract 5g, peptone 10g, NaCl 10g add distilled water To 1000ml, pH 7.0；Condition of culture is 37 DEG C, 200rpm, incubation time 8h；Saccharomyces cerevisiae seed culture uses YPD yeast Complete medium: yeast extract 10g, peptone 20g, glucose 20g are settled to 1L, pH 7.0；Condition of culture is 30 DEG C, 200rpm, incubation time 12h；Lactobacillus lactis seed culture is prepared using MRS culture medium: peptone 10g, beef extract 10g, yeast Bacterium powder 5g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, ammonium citrate 2g, magnesium sulfate 0.2g, manganese sulfate 0.2g, agar 20g, tween 801ml, glucose 20g (because glucose and peptone react therefore need to individually prepare), dissolve respectively, add distilled water extremely 1000ml, pH value are adjusted to 6.2-6.6 with glacial acetic acid, and condition is 37 DEG C, 200rpm, incubation time 10h.
Radix cynanchi bungei waste material culture medium is prepared, and radix cynanchi bungei waste material culture medium is prepared, 2000g radix cynanchi bungei waste material, 10g brown sugar, 500ml water, waste material and radix cynanchi bungei straw powder 3:1 in radix cynanchi bungei waste material after radix cynanchi bungei root tuber extracting active material.By gemma bar Bacterium, yeast and Bacillus acidi lactici are inoculated into culture medium respectively, are inoculated with according to 10% amount, and 28 DEG C, ferment 15d；After fermentation, fermentation Object filtering, filtrate are centrifuged under the conditions of 10000rpm at 4 DEG C, obtain supernatant, as enzyme agent.
It is prepared by radix cynanchi bungei root tuber waste material and radix cynanchi bungei straw powder after 2 extracting active materials
After water extracts, the radix cynanchi bungei root tuber waste material after extracting active material can be used directly.Radix cynanchi bungei straw powder system Make to crush using physical method first and washes down hydroxide with Natural Water with 2% naoh treatment 2h for the club of 5mm long Sodium dries.
3 enzyme agents degradation fruit-vegetable pesticide residues
Preparation of samples: sample 1, the vegetables cultivated using conventional method obtain the vegetable sample of non agricultural chemical residuum；Sample 2, Pesticide-treated is carried out to the vegetable sample of non agricultural chemical residuum, obtains the methyl sulfur bacterium phosphorus and lambda-cyhalothrin residual of 15ppm or so Vegetable sample；Sample 3, with enzyme agent to the remaining vegetable sample of methyl sulfur bacterium phosphorus and lambda-cyhalothrin of 15ppm or so, into Row processing, processing scheme are the methyl sulfur bacterium phosphorus and the remaining vegetable sample of lambda-cyhalothrin that 1L enzyme agent handles 15ppm or so, 25 DEG C, stewing process 10min, finally obtain the vegetable sample successively handled by pesticide-treated and enzyme agent.
Detecting Pesticide: Detecting Pesticide is carried out respectively to three kinds of samples, as the result is shown without pesticide sample and ferment Pesticide residue is 0 in agent treated sample, pesticide-treated and without the pesticide residues in vegetables concentration that enzyme agent is handled is 15ppm or so.Test the pesticide residues such as the methyl sulfur bacterium phosphorus containing 15ppm or so and lambda-cyhalothrin in fruits and vegetables sample, degradation Pesticide concentration be 300 times or so of the national trace standard of pesticide (0.05ppm).

Claims (5)

1. a kind of method for the cleaning agent for preparing fruits and vegetables pesticide with radix cynanchi bungei waste material, which is characterized in that radix cynanchi bungei root tuber is utilized Waste material and radix cynanchi bungei straw powder after extracting active material.
2. as described in claim 1, preparation method of the invention: (1) radix cynanchi bungei waste material culture medium is prepared, and 2000g radix cynanchi bungei is useless Material, 10g brown sugar, 500ml water mix；Sterilizing, 150 DEG C, 1.5 atmospheric pressure, 15min；(2) it ferments, successively access has food The bacillus pumilus (Bacillus pumulis) of safety, saccharomyces cerevisiae (Saccharomyces cerevisiae) and thermophilic Lactobacillus lactis (Lactobacillus acidophilus), is left to ferment 15d by 28 DEG C；(3) it separating, filter centrifugation obtains supernatant, Frozen drying obtains pulvis, is fruits and vegetables detergent for washing out agricultural chemicals, 4 DEG C of preservations after packing.
3. as claimed in claim 2, the waste material and radix cynanchi bungei stalk in radix cynanchi bungei waste material after radix cynanchi bungei root tuber extracting active material Powder 3:1.
4. as claimed in claim 2, access strain have foodsafety bacillus pumilus (Bacillus pumulis), Saccharomyces cerevisiae (Saccharomyces cerevisiae) and lactobacillus acidophilus (Lactobacillus acidophilus).
5. as claimed in claim 2, radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals such as contains methyl to desinsection class pesticide and bactericidal pesticide The bacterium such as sulphur bacterium phosphorus, lambda-cyhalothrin have apparent degradation effect, and 10mg vegetable detergent for washing out agricultural chemicals is dissolved in 1L tap water, 10min Remains of pesticide in interior degradable 1kg fruits and vegetables sample tests methyl sulfur bacterium phosphorus and chlorine fluorine containing 15ppm or so in fruits and vegetables sample The pesticide residues such as Cyano chrysanthemate, the pesticide concentration of degradation are 300 times or so of the national trace standard of pesticide (0.05ppm).
CN201710745934.1A 2017-08-28 2017-08-28 The preparation method of radix cynanchi bungei fruits and vegetables detergent for washing out agricultural chemicals Pending CN109423506A (en)

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