CN1094214A - Kelp spore suspension culture and immobilization are grown seedlings - Google Patents
Kelp spore suspension culture and immobilization are grown seedlings Download PDFInfo
- Publication number
- CN1094214A CN1094214A CN 93112423 CN93112423A CN1094214A CN 1094214 A CN1094214 A CN 1094214A CN 93112423 CN93112423 CN 93112423 CN 93112423 A CN93112423 A CN 93112423A CN 1094214 A CN1094214 A CN 1094214A
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- seedling
- spore
- gametophyte
- rope
- cultivation
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Abstract
The present invention relates to the seedling-cultivating method of sea-tangle, mainly solve kelp seedling cultivation and aestivate attached to low temperature on the fixation matrix because of spore and need a large amount of cooling waters, expend the very big problem of water power.Feature of the present invention is the spore that kind of band diffuses to be grown by it be gametophyte, with the inflation suspension method spore and gametophyte High Density Cultivation low temperature are aestivated, heat up to cultivate to 9-October and allow gametophyte grow to be sporophyte, when sporophyte cell is 10 left and right sides, to carry out immobilization and collect seedling through the ovulation after fertilization.The present invention only needs a small amount of chilled water, its water power expend only expend for the required water power of traditional seedling-cultivating method 1-10%, be suitable for kelp seedling cultivation and use.
Description
The present invention relates to the seedling-cultivating method of sea-tangle.
In the prior art seedling-cultivating method of a main laminaria be allow in 6-7 month kind of a band diffuse spore attached to fixation matrix on, for example attached on the rope, make spore development be gametophyte again low temperature aestivate, cultivate the formation juvenile sporophyte up to heating up the 10-11 month, be seedling, treat to put into after seedling grows to about 1 centimetre mariculture and carry out the breed of branch seedling again to 10-20 centimetre.This seedling-cultivating method since spore to go up the aestivate area that takies seedling rearing room of low temperature attached to rope big, need prepare a large amount of cooling waters every day and aestivate to guarantee gametophyte low temperature, and the low temperature time need continue 3-5 month, so water power expend huge.
The seedling-cultivating method that the purpose of this invention is to provide a main laminaria, this method do not allow spore anchor on the matrix, spore is aestivated at less solid space aestivate and need the problem of a large amount of cooling waters to overcome spore or gametophyte low temperature.
The present invention is that the spore that kind of band is diffused and the gametophyte of growth thereof aestivate spore and gametophyte High Density Cultivation low temperature with the inflation suspension method, and promptly making spore and gametophyte suspension, spore and gametophytic density with inflation is 10
4-10
8Spore/milliliter, the temperature of cultivating is 2-7 ℃, light intensity 300-1000 meter-candle, and periodic replacement fresh seawater, transferring PH is about 8, add nitrogen and phosphorous nutrient, to the cultivation that heats up in 9-10 month, cultivation temperature is 15-18 ℃, allow gametophyte grow and be sporophyte through the ovulation after fertilization, carrying out immobilization when sporophyte cell is seedling about 10 collects seedling, promptly adopt to drip and spill or immersion process is 50-200/centimetre by density seedling is adsorbed on the rope, drying in the shade with being added with sodium chloride proportion slightly is that 1.010 distilled water preparation 2-5% sodium alginate solution is evenly smeared or soaked rope with seedling again, or seedling and sodium alginate solution smeared after mixed or soak rope, then the seedling rope that is stained with sodium alginate being put into the seawater that is added with 0.1M calcium chloride stirs, forming glue-line until sodium alginate solidifies, again the seedling rope that solidifies is put into the fresh seawater rinsing and wash away unnecessary calcium chloride, support temporarily and put into mariculture or directly put into mariculture in 12-20 days.
Because the present invention adopts gametophyte suspension High Density Cultivation low temperature to aestivate, changed traditional growing seedlings spore has been gone up the method that low temperature is aestivated attached to rope, thereby only need a small amount of chilled water, its water power only expends and to be the prior art 1-10% that water power expends that grows seedlings, and the seedling of Pei Yanging guarantees that by the solid phase specific energy seedling density reaches production requirement simultaneously.
Realize that best way of the present invention is that the density of spore and gametophyte suspension culture is controlled at 10
4-10
6/ ml, temperature are 2-4 ℃, and seedling is 100/centimetre attached to the density on the rope.Attached to the seedling on the rope also can be the seedling of somatic cell seedling and the female reproduction of gametophyte arc.
Claims (1)
1, kelp spore suspension culture and immobilization are grown seedlings, it is characterized in that adopting the spore that kind of band diffuses and the gametophyte of growth thereof, with the inflation suspension method spore and gametophyte High Density Cultivation low temperature are aestivated, promptly making spore and gametophyte suspension, spore and gametophytic density with inflation is 10
4-10
8Individual/milliliter, the temperature of cultivating is 2-7 ℃, light intensity 300-1000 meter-candle, and periodic replacement fresh seawater, transferring PH is about 8, add nitrogen and phosphorous nutrient, to the cultivation that heats up in 9-10 month, cultivation temperature is 15-18 ℃, allow gametophyte grow and be sporophyte through the ovulation after fertilization, carrying out immobilization when sporophyte cell is seedling about 10 collects seedling, promptly adopt to drip and spill or immersion process is 50-200/centimetre by density seedling is adsorbed on the rope, drying in the shade with being added with sodium chloride proportion slightly is that 1.010 distilled water preparation 2-5% sodium alginate solution is evenly smeared or soaked rope with seedling again, or the seedling rope of seedling and sodium alginate is put into the seawater that is added with 0.1M calcium chloride stir, forming glue-line until sodium alginate solidifies, again the seedling rope that solidifies is put into the fresh seawater rinsing and wash away unnecessary calcium chloride, support temporarily and put into mariculture or directly put into mariculture in 12-20 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93112423 CN1094214A (en) | 1993-04-30 | 1993-04-30 | Kelp spore suspension culture and immobilization are grown seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93112423 CN1094214A (en) | 1993-04-30 | 1993-04-30 | Kelp spore suspension culture and immobilization are grown seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1094214A true CN1094214A (en) | 1994-11-02 |
Family
ID=4990037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93112423 Pending CN1094214A (en) | 1993-04-30 | 1993-04-30 | Kelp spore suspension culture and immobilization are grown seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1094214A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302704C (en) * | 2004-06-24 | 2007-03-07 | 山东东方海洋科技股份有限公司 | Improved sea weeds seedling cultivation process |
| CN100334204C (en) * | 2005-11-17 | 2007-08-29 | 山东东方海洋科技股份有限公司 | Process for solid phase cultivating preserving of Laminaria gamobium |
| CN100382676C (en) * | 2004-08-17 | 2008-04-23 | 中国科学院海洋研究所 | Method for comprehensively controlling kelp seedling takeoff or seedling decay by utilizing oligosaccharide solution |
| CN100387707C (en) * | 2006-01-20 | 2008-05-14 | 山东东方海洋科技股份有限公司 | Clone seedling breeding method for sea-tangle gametocyte |
| CN100494356C (en) * | 2005-05-20 | 2009-06-03 | 中国科学院海洋研究所 | Method for breeding energy alga-kelp sporophyte using gametophyte clone method |
| CN103548678A (en) * | 2013-10-24 | 2014-02-05 | 山东省海水养殖研究所 | Kelp gametophyte cloning device and culture method of kelp gametophyte |
| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
| CN108271680A (en) * | 2018-01-12 | 2018-07-13 | 中国水产科学研究院南海水产研究所 | A kind of Gloiopeltis algae holdfast freezen protective seedling method |
| CN109329143A (en) * | 2018-10-25 | 2019-02-15 | 青岛农业大学 | A kind of mussel attachment base kelp seedling normal-temperature breeding method |
| CN110291983A (en) * | 2019-06-26 | 2019-10-01 | 山东省海洋生物研究院 | A kind of Inoculation Method of sargassum kjellmanianum Yendo algal reef |
-
1993
- 1993-04-30 CN CN 93112423 patent/CN1094214A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302704C (en) * | 2004-06-24 | 2007-03-07 | 山东东方海洋科技股份有限公司 | Improved sea weeds seedling cultivation process |
| CN100382676C (en) * | 2004-08-17 | 2008-04-23 | 中国科学院海洋研究所 | Method for comprehensively controlling kelp seedling takeoff or seedling decay by utilizing oligosaccharide solution |
| CN100494356C (en) * | 2005-05-20 | 2009-06-03 | 中国科学院海洋研究所 | Method for breeding energy alga-kelp sporophyte using gametophyte clone method |
| CN100334204C (en) * | 2005-11-17 | 2007-08-29 | 山东东方海洋科技股份有限公司 | Process for solid phase cultivating preserving of Laminaria gamobium |
| CN100387707C (en) * | 2006-01-20 | 2008-05-14 | 山东东方海洋科技股份有限公司 | Clone seedling breeding method for sea-tangle gametocyte |
| CN103548678B (en) * | 2013-10-24 | 2015-04-15 | 山东省海水养殖研究所 | Kelp gametophyte cloning device and culture method of kelp gametophyte |
| CN103548678A (en) * | 2013-10-24 | 2014-02-05 | 山东省海水养殖研究所 | Kelp gametophyte cloning device and culture method of kelp gametophyte |
| CN103651095A (en) * | 2013-12-26 | 2014-03-26 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
| CN103651095B (en) * | 2013-12-26 | 2015-03-11 | 安徽理工大学 | Large-scale seaweed seedling culturing and transplanting method |
| CN108271680A (en) * | 2018-01-12 | 2018-07-13 | 中国水产科学研究院南海水产研究所 | A kind of Gloiopeltis algae holdfast freezen protective seedling method |
| CN109329143A (en) * | 2018-10-25 | 2019-02-15 | 青岛农业大学 | A kind of mussel attachment base kelp seedling normal-temperature breeding method |
| CN110291983A (en) * | 2019-06-26 | 2019-10-01 | 山东省海洋生物研究院 | A kind of Inoculation Method of sargassum kjellmanianum Yendo algal reef |
| CN110291983B (en) * | 2019-06-26 | 2021-09-14 | 山东省海洋生物研究院 | Artificial inoculation method for sargassum muticum reef |
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| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |