CN100387707C - Clone seedling breeding method for sea-tangle gametocyte - Google Patents
Clone seedling breeding method for sea-tangle gametocyte Download PDFInfo
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- CN100387707C CN100387707C CNB2006100422649A CN200610042264A CN100387707C CN 100387707 C CN100387707 C CN 100387707C CN B2006100422649 A CNB2006100422649 A CN B2006100422649A CN 200610042264 A CN200610042264 A CN 200610042264A CN 100387707 C CN100387707 C CN 100387707C
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Abstract
The present invention discloses a seedling culture method for sea-tangle gametocyte clones. The method takes gametocyte clones as seed sources, and solves the problems that the seedling culture time affected by the water temperature of a sea area is long, and the seed sea-tangle seedling culture causes the confusion of sea-tangle varieties, and the occurrence of different diseases in the process of seeding culture caused by the difference between the seed sea-tangle growth degree and the decline situation. The problems are caused by using ripe seed sea-tangle as a seed source in the past. Because the seedling collection of the sea-tangle is completed through two times and a double-layer curtain is adopted, the seeding culture production capacity of the sea-tangle is increased.
Description
One, technical field
The invention belongs to marine organisms seeding raising technology field, especially relate to a kind of clone seedling breeding method for sea-tangle gametocyte.
Two, background technology
The main laminaria method for culturing seedlings that kelp seedling cultivation still follows conventional lines now, this method is before the natural waters water temperature is raised to 23 ℃ (Zhifu gulf mid-July), take the ripe main laminaria of choosing in advance indoor, wash the back and diffuse the trip spore with the low temperature seawater stimulation, add attaching substratum after reaching certain density, after the trip spore passes through moving about of certain hour, initiatively attached on the matrix, under suitable conditions such as water temperature light, can begin to grow, through trimestral cultivation, the sea-tangle seedling grows into 2 centimetres, and the sea area natural temperature is stabilized in (Zhifu gulf mid-October) below 19 ℃ and the sea-tangle seedling is gone out the pond moves on to and marinely support temporarily.The problem that this method exists: the one, seedling raise period is influenced by the sea area water temperature, and seedling raise period is long; The 2nd, main laminaria is grown seedlings and is caused the sea-tangle variet complexity; The 3rd, the development degree of main laminaria and the mashed situation difference that declines cause the generation of different diseases in the seedling raising process.
The laminaria gametophytic clone seedling method has solved above-mentioned problem, and seeding raising technology is mature on the whole, but this new technology is difficult to promote.Cause the reason of this situation: the one, because the kelp seedling cultivation technology that this method is had relatively high expectations, the 2nd, can only use the individual layer curtain in this method seedling raising process, and ordinary method can have 30～40% double-deck curtain, causes clone seedling breeding output than the ordinary method underproduction more than 30%.
Three, summary of the invention
The objective of the invention is to improve the deficiency of prior art and provide a kind of utilize gametophyte clone as provenance, shorten that seedling raise period 30%, sea-tangle seedling output do not reduce, the method for culturing seedlings simple possible, can reach the clone seedling breeding method for sea-tangle gametocyte of the purpose that reduces cost, increases benefit.
The object of the present invention is achieved like this, a kind of clone seedling breeding method for sea-tangle gametocyte, be characterized in this method be with gametophyte clone as provenance, through following steps:
A, clone collect seedling and carried out mid-July to August 20, occupy the individual layer seedling medium closely before collecting seedling in nursery pond, and temperature of fry nursing pool is reduced to 7.0 ℃, stop refrigeration and recirculated water;
B, to collect seedling be with the mixed by 2: 1 of cultured female and male gametophytes clone in advance, is sprayed onto equably on the water surface of nursery pond, and gametophyte clone is attached on the seedling medium under the effect of gravity gradually;
C, 36 hours rear clones adhere to end, and beginning recirculated water is to keep water temperature at 7～7.5 ℃.The sprinkling amount of megagametophyte is 5 gram/curtains;
D, through 10-20 days cultivations, gametophyte is converted into sporophyte, the part sporophyte stable attached on the seedling medium time, wash away seedling medium with water pump, unnecessary sporophyte washed away and collect;
E, the seedling medium that washes away end increase by 50% double-deck curtain on the basis that was spread in a single layer originally, on the position of vacating, occupy the individual layer seedling medium again, stop recirculated water;
F, evenly be sprayed onto seedling medium on by the amount of 15 gram/curtains collected sporophyte, begin recirculated water after 36 hours, after collecting seedling ten days, secondary the double-deck curtain of collecting seedling for the first time can be evenly spread on all seedling curtains of collecting seedling for twice, thereby make the ratio of cloning the double-deck curtain of collecting seedling reach 33%, finish the gametophyte clone work of collecting seedling of growing seedlings, enter the conventional seedbed system production management stage.
Compared with the prior art the present invention has following distinguishing feature and positively effect: the present invention adopts gametophyte clone as provenance, having changed the seedling raise period that in the past utilizes ripe main laminaria to exist as provenance is subjected to that the sea area water temperature influences, seedling raise period long, main laminaria is grown seedlings and causes the development degree of sea-tangle variet complexity, main laminaria and the mashed situation difference that declines, cause the generation problem of different diseases in the seedling raising process, sea-tangle is collected seedling and carries out at twice and adopt double-deck curtain, has improved kelp seedling cultivation output.
Four, specific embodiments
Embodiment, a kind of clone seedling breeding method for sea-tangle gametocyte, with northern Shandong Bay areas is example, August 9, the beginning circulating water cooling, and will wash good neat being placed in the nursery pond of sea-tangle seedling curtain of pre-treatment, second day, the female and male gametophytes clone 30kg that stimulates is in advance smashed with high-speed tissue mashing machine, be twisted into little cell section by hand with 400 mesh sieve thin,tough silk then, be sprayed onto uniformly on 4000 sea-tangle seedling curtains with atomizer, with topped up with water in the nursery pond, stop circulation before the sprinkling clone, morning August 12, the clone adhered to substantially, beginning recirculated water provides suitable illumination, water temperature, water quality, nutrition promotes clone's growth; August 15, gametophyte germinated into sporophyte, and August 25, sporophyte was grown to 4 row cells, and the part sporophyte is adhere firmly, washes away the seedling curtain with water pump, and collected because of adhering to the insecure sporophyte that comes off; To wash away the seedling curtain of end and put double-deck curtain, and on the position of vacating, put again and wash and 1330 on the good sea-tangle seedling curtain of pre-treatment according to 50% ratio.The sporophyte the collected weight according to 15 gram/curtains evenly is sprayed onto with watering can on the sea-tangle seedling curtain of newly putting, stops recirculated water before spraying the clone, began recirculated water on August 27; September 6 was assigned to whole workshop uniformly with all double-deck curtains, began normal seedling management.To October 10 the sea-tangle seedling grow into 1.5～2.0cm, reach the outbound specification.
The present invention need be different according to various places natural condition, select the suitable time to grow seedlings.
Claims (1)
1. clone seedling breeding method for sea-tangle gametocyte, it is characterized in that this method be with gametophyte clone as provenance, through following steps:
A, clone collect seedling and carried out mid-July to August 20, closely occupy the individual layer seedling medium before collecting seedling in nursery pond, and temperature of fry nursing pool is reduced to 7.0 ℃, stop refrigeration and recirculated water;
B, to collect seedling be with the mixed by 2: 1 of cultured female and male gametophytes clone in advance, is sprayed onto equably on the water surface of nursery pond, and the sprinkling amount of megagametophyte is 5 gram/curtains, and gametophyte clone is attached on the seedling medium under the effect of gravity gradually;
C, 36 hours rear clones adhere to end, and beginning recirculated water is to keep water temperature at 7～7.5 ℃;
D, through 10-20 days cultivations, gametophyte is converted into sporophyte, the part sporophyte stably attached on the seedling medium time, washes away seedling medium with water pump, unnecessary sporophyte is washed away and collects;
E, the seedling medium that washes away end increase by 50% double-deck curtain on the basis that was spread in a single layer originally, on the position of vacating, occupy the individual layer seedling medium again, stop recirculated water;
F, evenly be sprayed onto seedling medium on by the amount of 15 gram/curtains collected sporophyte, begin recirculated water after 36 hours, after secondary was collected seedling ten days the double-deck curtain of collecting seedling is for the first time evenly spread on all seedling curtains of collecting seedling for twice, thereby make the ratio of cloning the double-deck curtain of collecting seedling reach 33%, finish the gametophyte clone work of collecting seedling of growing seedlings, enter the conventional seedbed system production management stage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100422649A CN100387707C (en) | 2006-01-20 | 2006-01-20 | Clone seedling breeding method for sea-tangle gametocyte |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100422649A CN100387707C (en) | 2006-01-20 | 2006-01-20 | Clone seedling breeding method for sea-tangle gametocyte |
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| Publication Number | Publication Date |
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| CN1807598A CN1807598A (en) | 2006-07-26 |
| CN100387707C true CN100387707C (en) | 2008-05-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2006100422649A Expired - Fee Related CN100387707C (en) | 2006-01-20 | 2006-01-20 | Clone seedling breeding method for sea-tangle gametocyte |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548678B (en) * | 2013-10-24 | 2015-04-15 | 山东省海水养殖研究所 | Kelp gametophyte cloning device and culture method of kelp gametophyte |
| CN103766214B (en) * | 2014-01-23 | 2016-09-07 | 山东东方海洋科技股份有限公司 | One main laminaria heavy ion radiation method for mutation breeding |
| CN105210845B (en) * | 2015-10-30 | 2017-12-05 | 中国科学院海洋研究所 | A kind of method for detecting variet complexity in sea-tangle summer sporelings (of laminaria) refrigeration hydraulic art |
| CN105706893A (en) * | 2016-02-03 | 2016-06-29 | 大连海宝渔业有限公司 | Cloned undaria pinnatifida seedling attachment method |
| CN106035052B (en) * | 2016-07-27 | 2018-12-25 | 山东大学（威海） | One main laminaria regrowth hair seedling method for culturing seedlings |
| CN108552046A (en) * | 2018-04-23 | 2018-09-21 | 山东东方海洋科技股份有限公司 | A kind of seedling cultivation method for sea-tangle for reducing production cost |
| CN110463598B (en) * | 2019-08-30 | 2021-06-25 | 中国水产科学研究院黄海水产研究所 | Method for cultivating Laminaria japonica seedlings by utilizing gametophyte clone line |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094214A (en) * | 1993-04-30 | 1994-11-02 | 上海水产大学 | Kelp spore suspension culture and immobilization are grown seedlings |
| CN1342401A (en) * | 2000-09-08 | 2002-04-03 | 青岛海洋大学 | Gametophyte cloning method of breeding heterotic kelp |
| JP2004121162A (en) * | 2002-10-07 | 2004-04-22 | Takeo Yamaguchi | Fish bank for tangle cultivation |
-
2006
- 2006-01-20 CN CNB2006100422649A patent/CN100387707C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094214A (en) * | 1993-04-30 | 1994-11-02 | 上海水产大学 | Kelp spore suspension culture and immobilization are grown seedlings |
| CN1342401A (en) * | 2000-09-08 | 2002-04-03 | 青岛海洋大学 | Gametophyte cloning method of breeding heterotic kelp |
| JP2004121162A (en) * | 2002-10-07 | 2004-04-22 | Takeo Yamaguchi | Fish bank for tangle cultivation |
Non-Patent Citations (2)
| Title |
|---|
| 海带育种育苗技术研究进展. 冯蕾等.科学技术与工程，第5卷第8期. 2005 |
| 海带育种育苗技术研究进展. 冯蕾等.科学技术与工程，第5卷第8期. 2005 * |
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| CN1807598A (en) | 2006-07-26 |
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