CN109414399B

CN109414399B - Cosmetic composition for skin irritation reduction and skin inflammation reduction comprising hydrangea fermentation product

Info

Publication number: CN109414399B
Application number: CN201780040265.6A
Authority: CN
Inventors: 李敬银; 柳正镇; 姜承贤; 金延俊
Original assignee: Cosmax Inc
Current assignee: Cosmax Inc
Priority date: 2016-06-30
Filing date: 2017-06-30
Publication date: 2020-10-30
Anticipated expiration: 2037-06-30
Also published as: KR20180003664A; WO2018004293A2; CN109414399A; WO2018004293A3; KR101867308B1

Abstract

The present invention relates to a cosmetic composition for skin irritation reduction and skin inflammation reduction comprising fermented product of hydrangea macrophylla as an active ingredient. According to the present invention, the cosmetic composition comprising the fermented product of hydrangea macrophylla of the present invention as an active ingredient relieves skin irritation and skin inflammation by inhibiting the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1 β), and is thus finally effectively applicable to the use for preventing and improving skin allergy, improving redness, preventing and improving dermatitis, and the like.

Description

Cosmetic composition for skin irritation reduction and skin inflammation reduction comprising hydrangea fermentation product

Technical Field

The present invention relates to a cosmetic composition for skin inflammation relief, and more particularly, to a cosmetic composition for skin irritation relief and skin inflammation relief comprising hydrangea fermentation product.

Background

One of the most important functions of the skin is a protective function as a barrier. Skin, which is directly exposed to the external environment, is the first line of defense of primary importance in preventing loss of body fluids and protecting the body from harmful environments. And, performs a barrier function against toxic substances or microorganisms, physical stimulus, or ultraviolet rays. However, when such skin barrier function is subjected to various external and internal skin irritations, the skin barrier is damaged, the skin becomes dry, or the skin is subjected to an inflammatory reaction. Meanwhile, if the initial inflammatory reaction occurring at this time cannot be effectively controlled, it may be worsened to severe dermatitis.

However, as the population grows, urbanization, industrialization, and automobiles increase, air pollution becomes serious, and the types of pollutants become various. Particularly, due to the location factor in korea, it is faced with the damage of yellow sand, fine dust, and the like caused by the movement of air pollutants discharged from china, and the severity thereof also becomes high. The fine dust (particulate matter) is classified into fine dust (PM10) having a diameter of 10 μm or less and ultrafine dust (PM2.5) having a diameter of 2.5 μm or less according to the particle size. The korean ministry of environment limits PM10 to air pollutants. It is known that such atmospheric pollutants as fine dust induce respiratory diseases and heart diseases, and at the same time, the influence on the skin occupying a wide area in the body is receiving increasing attention. In particular, it is reported that fine dust in air pollutants deteriorates the epidermal barrier function and worsens allergic dermatitis. Also, sensitive skin reacts more sensitively to external stimuli or environmental changes than general skin, thereby exhibiting irritation or dermatitis symptoms. It has been found that people with such sensitive skin are more sensitive to the extent of mote-induced irritation than normal people, and can experience more than three times the irritation. Therefore, the possibility that the contaminant such as the fine dust causes a skin problem particularly to a person having sensitive skin is high. It also affects skin aging, and according to recent epidemiological studies, the appearance of increased wrinkles and spots in skin exposed to fine dust is directly associated with aging. Active oxygen is produced by the dust particles to inhibit collagen synthesis and activate decomposition, and Polycyclic Aromatic Hydrocarbons (PAH) adsorbed on the dust particles activate organism foreign body metabolism through AhR to increase collagen decomposition, thereby causing skin aging. Thus, a method of reducing direct contact between fine dust and the skin and a new therapeutic method through studies related to molecular mechanisms should be searched for.

Inflammatory response (inflammation response) in the skin is responsible for the role of defense against physical stimuli or chemical substances, bacteria, etc., when skin is damaged, and various immune cells and inflammation-inducing cytokines are involved therein. Interleukin-6 (IL-6, interleukin-6), interleukin-8 (IL-8, interleukin-8), interleukin-1 beta (IL-1 beta, interleukin-beta) and the like are representative inflammation-inducing cytokines. IL-1 β is a cytokine that is typically formed by activated mononuclear phagocytes, epithelial cells, and vascular endothelial cells to mediate inflammatory responses, and IL-6 is a cytokine that is secreted by T cells and macrophages to stimulate immune responses and regulate inflammatory responses. Also, not only the inflammation-inducing cytokine, but also activated macrophages (macrophages) excessively generate Nitric Oxide (NO) or prostaglandin (prostagladin) E2(PGE2), thereby further activating the inflammatory process. It has been reported that the accelerated and anti-inflammatory mote particles increase the expression of such inflammation-inducing cytokines in human keratinocytes.

In the past, attempts have been made in various ways to develop cosmetics that can impart an anti-inflammatory function to the skin using natural extracts, and typical examples thereof are as follows: cosmetic compositions comprising camellia extract are proposed in korean laid-open patent No. 2007-. Furthermore, Korean laid-open patent No. 2015-0078948 proposes a cosmetic composition for skin inflammation improvement or skin irritation alleviation comprising a mixed extract of mistletoe, pine bark and crocus sativus as an active ingredient, and Korean laid-open patent No. 2009-0091547 proposes a cosmetic composition for anti-inflammation and skin irritation alleviation comprising a mixed extract of Ecliptae herba and cnidium monnieri. However, none of the above cosmetic compositions describes the skin irritation-reducing and skin inflammation-reducing effects of the fermented extract of hydrangea of the present invention.

In view of the above, the present inventors have made extensive studies to overcome the problems of the prior art described above, and finally have identified that inflammatory cytokines increased by various stimuli, particularly, by fine dust stimuli, are associated with skin irritation and skin inflammation, and have screened and compared plant extracts for inhibiting the expression of inflammatory cytokines in order to discover substances that improve such problems, and have confirmed that fermented products of fermented hydrangea macrophylla extracts have the efficacy of alleviating skin irritation and skin inflammation by inhibiting the expression of IL-6, IL-8, and IL-1 β, which are inflammation-inducing cytokines, thereby completing the present invention.

Disclosure of Invention

Technical problem

Accordingly, a primary object of the present invention is to provide a cosmetic composition for relieving skin inflammation, comprising fermented product of hydrangea macrophylla having efficacy of relieving skin irritation and skin inflammation by suppressing the expression of IL-6, IL-8 and IL-1 β, which are inflammation-inducing cytokines, increased by various stimuli, particularly by fine dust stimulation.

Another object of the present invention is to provide a cosmetic composition in which the fermented product of hydrangea macrophylla of the present invention is applied as a base formulation of a functional cosmetic composition to impart skin irritation alleviation and skin inflammation alleviation effects, thereby achieving skin allergy prevention and improvement, redness improvement, and dermatitis prevention and improvement.

Technical scheme

According to one embodiment of the present invention, there is provided a cosmetic composition for skin irritation reduction and skin inflammation reduction comprising fermented product of hydrangea macrophylla as an active ingredient.

Hydrangea (Hydrangea macrophylla) is used as deciduous shrub of Saxifragaceae of Rosales, blooms in 6-7 months, and initially is light purple, later becomes sky blue, and finally becomes light red. If the soil is neutral, the flower is white, if the soil is acidic, the flower is blue, and if the soil is alkaline, the flower is pink. As deciduous shrubs, the egg-shaped leaves are opposite, the edges of the shrubs are provided with sawteeth, the length of the shrubs is 10-15 cm, and the tips of the shrubs suddenly become sharp. The flower is commonly called garden tree because of being opened in rich summer at first summer, but the flower also has the well-known efficacy as a traditional Chinese medicine for treating heart diseases and an antipyretic. The whole grass containing roots is mainly used. Further, whitening effects (korean laid-open patent No. 2014-. However, the improvement effect of skin inflammation to external irritation of fermented extract of hydrangea macrophylla as fermented by pear flower according to the present invention is not mentioned either.

In view of the above, the present inventors have confirmed that skin irritation and skin inflammation can be alleviated by inhibiting the expression of inflammation-inducing cytokines by hydrangea fermentation products, and have completed the present invention.

The invention is characterized in that the hydrangea fermented product is obtained by fermenting hydrangea extract by utilizing pear flower yeast.

The term "fermentation using pear flower koji" refers to mixing pear flower koji with water for culture, and specifically refers to mixing pear flower koji 10%, hydrangea extract 1%, and water 89% at 25 deg.C, and performing anaerobic culture for 4 days.

The invention is characterized in that the above pear flower koji is rice koji, preferably prepared by inoculating a koji culture and a yeast liquid culture in a rice cake.

The Koji culture is a solid culture (Koji) prepared by sterilizing rice adjusted to 38-42% water at 121 deg.C for 30 min, inoculating Aspergillus oryzae (Aspergillus oryzae), culturing, and drying. The yeast liquid culture (Saccharomyces cerevisiae) is a culture obtained by culturing yeast in a potato dextrose (potato dextrose) liquid medium.

The present invention is characterized in that the above hydrangea extract can be extracted using any solvent conventionally used, and preferably, is extracted using water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof.

As the extraction method using the above extraction solvent, a general extraction method of plants, for example, a hot water extraction, a reflux cooling extraction, an ultrasonic extraction, a supercritical extraction, etc. can be used, specifically, hydrangea macrophylla is crushed to prepare a powder, and immersed in the above extraction solvent, and extracted at room temperature for 24 to 72 hours.

The present invention is characterized by comprising 0.001 to 30 weight percent of the above fermented product of hydrangea macrophylla relative to the total weight of the cosmetic composition. When the content of the fermented product of hydrangea macrophylla is less than 0.001 wt%, the effect of alleviating the skin inflammation may be weak, and thus it is not preferable, and on the contrary, when the content of the fermented product of hydrangea macrophylla is more than 30 wt%, it is not preferable in an economical aspect.

The present invention is characterized in that the above fermented product of hydrangea macrophylla is used to relieve skin irritation and skin inflammation by inhibiting inflammatory cytokines.

According to the experimental examples of the present invention, it was confirmed that the suppression effect of inflammatory cytokines increased by dust was observed, and as a result, it was confirmed that the fermented product of hydrangea of the present invention exhibited excellent suppression effect, and in particular, it was confirmed that the skin inflammation suppression effect was more excellent than that of the unfermented extract of hydrangea. From this result, it can be seen that the fermented product of hydrangea of the present invention can relieve not only skin inflammation by inhibiting inflammation-inducing cytokines but also skin irritation such as fine dust.

Further, as a result of confirming the effect of alleviating the epidermal layer irritation due to the fine dust, it was found that the epidermal layer and epidermal cells damaged by the irritation were recovered in the fermented product of hydrangea macrophylla of the present invention. From this result, it can be seen that the hydrangea fermentation can relieve the damage of the epidermis layer caused by the stimulation due to the fine dusts.

The present invention is characterized in that the cosmetic composition is in the form of one or more formulations selected from the group consisting of softening lotion, nourishing essence, nourishing oil, moisturizing oil, nourishing cream, pressed powder, pack, foundation, makeup base, cleansing cream, shampoo, milky lotion, and ointment.

The ingredients contained in the cosmetic composition of the present invention may contain, as active ingredients, various ingredients commonly used in cosmetic compositions, for example, conventional auxiliary agents and carriers such as stabilizers, solubilizers, vitamins, pigments and perfumes, in addition to the above-mentioned fermented product.

ADVANTAGEOUS EFFECTS OF INVENTION

As described above, the fermented product of hydrangea macrophylla according to the present invention can effectively inhibit the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1. beta.) overexpressed by various stimuli, particularly, by fine dusts, to alleviate skin irritation and skin inflammation.

Drawings

FIG. 1 is a graph showing the results of Experimental example 1 in which the degree of inhibition of IL-6 gene expression is exhibited in an inflammation-inducing cytokine.

FIG. 2 is a graph showing the results of Experimental example 1 in which the degree of inhibition of IL-8 gene expression is exhibited in an inflammation-inducing cytokine.

FIG. 3 is a graph showing the results of Experimental example 1 in which the degree of suppression of the expression of IL-1. beta. gene in inflammation-inducing cytokine is exhibited.

FIG. 4 is a photograph showing the results of measurement of recovery of fermented extract of hydrangea macrophylla by treating the cuticular layer damaged by fine dust.

Detailed Description

The present invention will be described in more detail below with reference to examples. These examples are merely illustrative of the present invention, and thus the scope of the present invention is not construed as being limited to these examples.

Example 1: preparation method of hydrangea macrophylla extract

After powdering hydrangea, each 100g of the extract was put into a separate container, immersed in 1L of 70 weight percent aqueous ethanol as an extraction solvent, and left to stand at room temperature for 24 hours to obtain an extract. The obtained extract was filtered with filter paper, and then the filtrate was concentrated under reduced pressure with a rotary evaporation dryer to obtain an extract.

Example 2: preparation of fermented product of hydrangea macrophylla

The hydrangea fermented product is prepared by fermenting the hydrangea extract obtained in the above example 1 using pear flower koji. The present invention is characterized in that the pear flower koji uses two kinds of species of bacteria, i.e., a koji culture and a yeast liquid culture.

The Koji culture is a solid culture (Koji) prepared by sterilizing rice adjusted to 38-42% water at 121 ℃ for 30 minutes, inoculating Aspergillus oryzae (Aspergillus oryzae), culturing, and drying. A yeast liquid culture (Saccharomyces cerevisiae) refers to a culture in which yeast is cultured in potato dextrose (potato dextrose) liquid medium.

The pear flower yeast is prepared by the following method, firstly, drying 100 parts by weight of cleaned rice for 20-24 hours at the temperature of 60 ℃, and then crushing. Recovering the powder which passes through a sieve of about 40 meshes, adding water in a manner that the moisture content reaches 40-45%, and then making the powder into a round shape for molding. After reforming the round rice into a hole-shaped rice cake, it was cooked at 100 ℃ for 1 hour. The two koji cultures and the yeast liquid culture which were previously cultured were inoculated to 1% of each weight and mixed. Aerobic culture was carried out at 25 ℃ for 4 days to complete the pear blossom koji.

The fermented product of hydrangea macrophylla is prepared by mixing 10% of a pear flower koji, 1% of an hydrangea macrophylla extract and 89% of water and anaerobically culturing at 25 ℃ for 4 days, and is used in experimental example 1 below.

Experimental example 1: evaluation of anti-inflammatory efficacy

In order to evaluate the anti-inflammatory effect of the fermented extract, the expression level of IL-1. beta. IL-6, IL-8, which are inflammation-inducing cytokines, was measured in the treated dust (PM 10). More specifically, HaCaT cells, which were keratinocytes, were treated with fine dust, and the final concentrations of the respective raw materials prepared in examples 1 and 2 were adjusted to 10. mu.g/ml, followed by culturing for 4 hours. Subsequently, mRNA was extracted from the cells, synthesized as cDNA, and real-time Polymerase Chain Reaction (PCR) was performed using a target template (primer), thereby finally evaluating the degree of gene expression of inflammatory cytokines. The results are shown in FIGS. 1 to 3.

As a result, as shown in fig. 1 to 3, when each cytokine is induced by fine dust, the inhibition degree of the hydrangea extract and the hydrangea fermented extract can be evaluated, and the pear flower fermented extract exhibits the highest and significant induction inhibition effect on all cytokines. Therefore, it was confirmed that the fermented extract of the present invention is a very useful raw material for a cosmetic composition having a higher anti-inflammatory effect than that before fermentation.

Experimental example 2: evaluation of epidermal irritation-relieving Effect

In order to evaluate the skin epidermal irritation relieving effect, the skin epidermal layer irritated by dust particles and damaged by the irritation relieving effect was evaluated by using three-dimensionally cultured artificial skin tissues. The hydrangea extracts and the hydrangea fermented extracts obtained in example 1 and example 2 were treated in a culture medium at a concentration of 10 μ g/ml by applying 50 μ g/ml of dust and fine dusts (PM10) to the surface of the artificial skin. After 24 hours of additional culture, the cells were fixed in formalin, and then paraffin blocks (paraffin blocks) were prepared for H & E staining. Thus, the morphology of the horny layer and keratinocytes in the epidermal layer was measured. The measurement results are shown in fig. 4.

As a result, as shown in fig. 4, it was confirmed that the stratum corneum of the artificial skin tissue damaged by the treatment with fine dust was relieved. Also, it was confirmed that the density and shape of the keratinocytes of the basal layer were restored. Therefore, it was confirmed that the fermented extract of the present invention is a very useful raw material for a cosmetic composition having a stimulation-relieving effect on the epidermis damaged by stimulation.

Dosage form example 1: preparation of astringent

A cosmetic lotion containing the fermented extract obtained in the above example 2 was prepared by a conventional method according to the composition components and composition ratios shown in the following table 1.

TABLE 1

Dosage form example 2: preparation of nutrient essence

A moisturizing cream containing the fermented extract obtained in example 2 was prepared by a conventional method according to the composition components and composition ratios shown in the following table 2.

TABLE 2

Dosage form example 3: moisturizing cream

A moisturizing cream comprising the fermented extract obtained in example 2 was prepared by a conventional method according to the composition components and composition ratios shown in the following table 3.

TABLE 3

Industrial applicability

As described above, according to the present invention, the cosmetic composition comprising the fermented product of hydrangea of the present invention as an active ingredient relieves skin irritation and skin inflammation relief by inhibiting the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1 β), and thus can be finally effectively applied to the use for preventing and improving skin allergy, improving flushing, preventing and improving dermatitis, and the like.

Reference to the literature

(patent document 0001) KR 10-2007-

(patent document 0002) KR 10-2010-0086698A

(patent document 0003) KR 10-2013-0016930A

(patent document 0004) KR 10-2015-

(patent document 0005) KR 10-2009-0091547A

Claims

1. A cosmetic composition for alleviating skin irritation and inflammation, comprising as an active ingredient a fermented product of hydrangea macrophylla obtained by fermenting a fermented product of hydrangea macrophylla with a fermented product of hydrangea macrophylla 10%, a fermented product of hydrangea macrophylla 1.0% and water 89% at 25 ℃ and anaerobically culturing for 4 days, wherein the fermented product of hydrangea macrophylla is prepared by inoculating a yeast culture and a yeast liquid culture in a rice cake made of rice.

2. The cosmetic composition for skin irritation reduction and skin inflammation reduction according to claim 1, wherein the hydrangea macrophylla extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

3. The cosmetic composition for skin irritation reduction and skin inflammation reduction according to claim 1, wherein the hydrangea macrophylla extract is extracted with water, butylene glycol, propylene glycol, glycerin, or a mixed solvent thereof.

4. The cosmetic composition for skin irritation alleviation and skin inflammation alleviation as claimed in claim 1, wherein the fermented product of hydrangea macrophylla is used for alleviating skin irritation and skin inflammation by inhibiting the expression of IL-6, IL-8, IL-1 β of inflammation-inducing cytokines.

5. The cosmetic composition for skin irritation alleviation and skin inflammation alleviation as claimed in claim 1, wherein the fermented product of hydrangea macrophylla is used for alleviating skin irritation and skin inflammation by inhibiting inflammatory cytokines.

6. The cosmetic composition for skin irritation alleviation and skin inflammation alleviation according to claim 1, wherein the cosmetic composition for skin irritation alleviation and skin inflammation alleviation is selected from the group consisting of softening lotions, nourishing essences, nourishing oils, moisturizing oils, nourishing creams, pressed powders, masks, foundations, makeup creams, facial cleansers, shampoos, lotions, creams, and ointments.